bco2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bco2b
Entrez Gene ID	678554
Full Name	beta-carotene oxygenase 2b
Synonyms	bcdo2,wu:fk75b02,zgc:136769
General protein information	Preferred Names beta-carotene oxygenase 2b Names beta-carotene oxygenase 2b bcdo2a beta-carotene 15, 15-dioxygenase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040312.1	NP_001035402.1	beta-carotene oxygenase 2b

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ninaB	NP_650307.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008143	XP_317319.4
	BCO2	XP_001100991.2
Homo sapiens (human)	BCO2	NP_114144.4
Mus musculus (house mouse)	Bco2	NP_573480.1
Danio rerio (zebrafish)	LOC100330576	XP_002662060.3
Danio rerio (zebrafish)	bco2a	NP_001035402.1
Xenopus tropicalis (tropical clawed frog)	LOC100487224	XP_002942801.2
Pan troglodytes (chimpanzee)	BCO2	XP_001147647.1
Gallus gallus (chicken)	BCO2	XP_417929.2
Canis lupus familiaris (dog)	BCO2	XP_536572.3
Bos taurus (cattle)	BCO2	NP_001095457.2
Rattus norvegicus (Norway rat)	Bco2	NP_001121184.1
Danio rerio (zebrafish)	bco2l	NP_571874.1
Oryza sativa (rice)	Os01g0566500	NP_001043363.1
Xenopus tropicalis (tropical clawed frog)	bco2	NP_001006739.1

Gene Ontology
Bibliography

Related articles in PubMed

The evolution and functional divergence of the beta-carotene oxygenase gene family in teleost fish--exemplified by Atlantic salmon.
Helgeland H, Sandve SR, Torgersen JS, Halle MK, Sundvold H, Omholt S, V?ge DI
Gene543(2)268-74(2014 Jun)

BCDO2 acts as a carotenoid scavenger and gatekeeper for the mitochondrial apoptotic pathway.
Lobo GP, Isken A, Hoff S, Babino D, von Lintig J
Development (Cambridge, England)139(16)2966-77(2012 Aug)

The 13q and 11q B-cell chronic lymphocytic leukaemia-associated regions derive from a common ancestral region in the zebrafish.
Auer RL, Riaz S, Cotter FE
British journal of haematology137(5)443-53(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

erythropoiesis was not impaired but erythrocytes underwent apoptosis in BCDO2-deficient larvae
Title: BCDO2 acts as a carotenoid scavenger and gatekeeper for the mitochondrial apoptotic pathway.

The following bco2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bco2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33223	NM_001040312.1 Latest version!	Danio rerio beta-carotene oxygenase 2b (bco2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bco2b ( NM_001040312.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001035402.1 Danio rerio beta-carotene oxygenase 2b (bco2b), mRNA.

Price & Availability↑

CloneID	ODa33223
Clone ID Related Accession (Same CDS sequence)	NM_001040312.1
Accession Version	NM_001040312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Danio rerio(zebrafish)
Product	beta-carotene oxygenase 2b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC115259.1. ##Evidence-Data-START## Transcript exon combination :: BC115259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTTCTA TGAACCGCCT GAACAGCAGC AAATCTGGCT GGAAGAAAAC CAGGCCTAAG 
AATAAGGATC AATACTTTAC AGATGTTCAC GGTCTGCCAA GCATTGAGAA GCTGATATGT 
TCTGCAGACG AAACCCCAGA GCCCATTACG ACCACTATTA CAGGTAACGT CCCTAGCTGG 
ATCAAGGGCA ATTTCCTCAG GAACGGACCT GGGAAGTTTG AGATCGGAAG AAGCAGTTTC 
AACCACTGGT TTGATGGCAT GGCGCTTTTG CACCAGTTCC ACATTGAGGA TGGAAAAGTG 
ACCTACATGA GCCGATTCCT CAACAGCGAT AGCTATAAGG AAAACCTAGA GCATAACCGC 
ATAATAGTGT CTGAATTTGG CACAGTGGCA ATGCCAGATC CGTGCAAGAA CTTCTTCCAG 
CGCTTCCTCT CACGCTTTGA GCTGCCAAAA CTAAGTGACA ATGCAAATGT GAGCTTTGTT 
CAGTATAAAG GAGACTACTA TGTCAGCACA GAGACCAACT TCATGCACAA AATAGATCCA 
GAAACACTTG AAACAAAGGA AAAGGTGGAC TGGAGTAAAT TTATTGCGGT TAATGGGGCT 
ACAGCTCACC CTCATGTGGA TTCTGATGGA ACGACATACA ACATGGGCAA CTCTTACACT 
GTTAAAGGTG CTTTTTACAA CATAATAATG GTGCCCCCAA ACAAGGAAAA CCCAAATGAT 
ACATTGGAAG GAGCGTCAGT TGTGTGTTCA ATCCCATCAG AGGACAAGTC CAAACCGTCC 
TACTATCACA GCTTTGCCAT GTCAGAGAAC TATGTGGTAT TCATCGAGCA GCCAATCAAA 
ATGGACCTTC TGAAGATTGT GACAGGGAAA TTGCGTGGAA AAGGAATTAA TGAAGGTGTT 
TACTGGGACC CCAAACGAAA CACAGTTTTC CATGTGATCA ATAAGCGCAC AGGAAAGCTG 
AGCCTTGTCA AATATTACAC AAAGCCACTG TCAACCTTCC ACCAGATAAA CTGCTATGAA 
GACAATGGTT TCTTGATTAT GGACATGTGC AGTTCTGATG ATGGCCAAGC AATAAACAAC 
TATGTAATTC AGAATCTTAA AAAGTCTGGG AGTGCTTTAG ATGAGGTATA TAACACATTG 
TGCAGAGTGT TTCCTCGCCG TTTTGTCCTG CCATTAAACG TGGACAGTGA CACACCCCAT 
GGGGAAAATC TCATCACATG CTTCAACAGC ACTGCAACAG CAATCAAGAC AGACAATAAC 
AAGGTGTTTT GCACATATGA AGATCTCCAT GGTGAGGATC TCCATGAATA CGGTGGACTG 
GAATTTCCAC ATATCAACTA TTCAATGTGC AATTCACACC CCTACCGTTA CTTCTACGGA 
TGTGGCTTCA GACACCTGGT TGGAGACTCT CTCATTAAAA TGGACCTTCA AGGCAAACAA 
ATAAAGGTGT GGAAGCATGC AGGCATGTTT CCTTCAGAAC CGGTCTTTGT ATCTTCACCG 
GGTGCTGTAG AAGAGGATGA TGGAGTCATT CTGTCTGTGG TCATCACCCC TAATCAGGAC 
AAGAGCACCT TCCTTCTAGT GCTGGATGCG AAGACATTTG AAGAATTGGG CCGCGCTGAA 
GTACCAAAAA ACATTCCTTA TGGATTCCAC GGGACCTTCC AATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035402.1
CDS	48..1715
Translation	MSSMNRLNSSKSGWKKTRPKNKDQYFTDVHGLPSIEKLICSADETPEPITTTITGNVPSWIKGNFLRNGPGKFEIGRSSFNHWFDGMALLHQFHIEDGKVTYMSRFLNSDSYKENLEHNRIIVSEFGTVAMPDPCKNFFQRFLSRFELPKLSDNANVSFVQYKGDYYVSTETNFMHKIDPETLETKEKVDWSKFIAVNGATAHPHVDSDGTTYNMGNSYTVKGAFYNIIMVPPNKENPNDTLEGASVVCSIPSEDKSKPSYYHSFAMSENYVVFIEQPIKMDLLKIVTGKLRGKGINEGVYWDPKRNTVFHVINKRTGKLSLVKYYTKPLSTFHQINCYEDNGFLIMDMCSSDDGQAINNYVIQNLKKSGSALDEVYNTLCRVFPRRFVLPLNVDSDTPHGENLITCFNSTATAIKTDNNKVFCTYEDLHGEDLHEYGGLEFPHINYSMCNSHPYRYFYGCGFRHLVGDSLIKMDLQGKQIKVWKHAGMFPSEPVFVSSPGAVEEDDGVILSVVITPNQDKSTFLLVLDAKTFEELGRAEVPKNIPYGFHGTFQS

Target ORF information:

RefSeq Version	NM_001040312.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio beta-carotene oxygenase 2b (bco2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040312.1

ORF Insert Sequence:

ATGTCTTCTA TGAACCGCCT GAACAGCAGC AAATCTGGCT GGAAGAAAAC CAGGCCTAAG 
AATAAGGATC AATACTTTAC AGATGTTCAC GGTCTGCCAA GCATTGAGAA GCTGATATGT 
TCTGCAGACG AAACCCCAGA GCCCATTACG ACCACTATTA CAGGTAACGT CCCTAGCTGG 
ATCAAGGGCA ATTTCCTCAG GAACGGACCT GGGAAGTTTG AGATCGGAAG AAGCAGTTTC 
AACCACTGGT TTGATGGCAT GGCGCTTTTG CACCAGTTCC ACATTGAGGA TGGAAAAGTG 
ACCTACATGA GCCGATTCCT CAACAGCGAT AGCTATAAGG AAAACCTAGA GCATAACCGC 
ATAATAGTGT CTGAATTTGG CACAGTGGCA ATGCCAGATC CGTGCAAGAA CTTCTTCCAG 
CGCTTCCTCT CACGCTTTGA GCTGCCAAAA CTAAGTGACA ATGCAAATGT GAGCTTTGTT 
CAGTATAAAG GAGACTACTA TGTCAGCACA GAGACCAACT TCATGCACAA AATAGATCCA 
GAAACACTTG AAACAAAGGA AAAGGTGGAC TGGAGTAAAT TTATTGCGGT TAATGGGGCT 
ACAGCTCACC CTCATGTGGA TTCTGATGGA ACGACATACA ACATGGGCAA CTCTTACACT 
GTTAAAGGTG CTTTTTACAA CATAATAATG GTGCCCCCAA ACAAGGAAAA CCCAAATGAT 
ACATTGGAAG GAGCGTCAGT TGTGTGTTCA ATCCCATCAG AGGACAAGTC CAAACCGTCC 
TACTATCACA GCTTTGCCAT GTCAGAGAAC TATGTGGTAT TCATCGAGCA GCCAATCAAA 
ATGGACCTTC TGAAGATTGT GACAGGGAAA TTGCGTGGAA AAGGAATTAA TGAAGGTGTT 
TACTGGGACC CCAAACGAAA CACAGTTTTC CATGTGATCA ATAAGCGCAC AGGAAAGCTG 
AGCCTTGTCA AATATTACAC AAAGCCACTG TCAACCTTCC ACCAGATAAA CTGCTATGAA 
GACAATGGTT TCTTGATTAT GGACATGTGC AGTTCTGATG ATGGCCAAGC AATAAACAAC 
TATGTAATTC AGAATCTTAA AAAGTCTGGG AGTGCTTTAG ATGAGGTATA TAACACATTG 
TGCAGAGTGT TTCCTCGCCG TTTTGTCCTG CCATTAAACG TGGACAGTGA CACACCCCAT 
GGGGAAAATC TCATCACATG CTTCAACAGC ACTGCAACAG CAATCAAGAC AGACAATAAC 
AAGGTGTTTT GCACATATGA AGATCTCCAT GGTGAGGATC TCCATGAATA CGGTGGACTG 
GAATTTCCAC ATATCAACTA TTCAATGTGC AATTCACACC CCTACCGTTA CTTCTACGGA 
TGTGGCTTCA GACACCTGGT TGGAGACTCT CTCATTAAAA TGGACCTTCA AGGCAAACAA 
ATAAAGGTGT GGAAGCATGC AGGCATGTTT CCTTCAGAAC CGGTCTTTGT ATCTTCACCG 
GGTGCTGTAG AAGAGGATGA TGGAGTCATT CTGTCTGTGG TCATCACCCC TAATCAGGAC 
AAGAGCACCT TCCTTCTAGT GCTGGATGCG AAGACATTTG AAGAATTGGG CCGCGCTGAA 
GTACCAAAAA ACATTCCTTA TGGATTCCAC GGGACCTTCC AATCATAG