MAPK14A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAPK14A
Entrez Gene ID	65237
Full Name	mitogen-activated protein kinase 14a
Synonyms	MAPK14,fk28c03,hm:zeh1243,p38,p38a,wu:fk28c03,zp38a
General protein information	Preferred Names mitogen-activated protein kinase 14a Names mitogen-activated protein kinase 14A MAP kinase 14A MAP kinase p38a MAPK 14A etID18993.8 mitogen-activated protein kinase p38a zeh1243
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131722.1	NP_571797.1	mitogen-activated protein kinase 14A
XM_005167061.4	XP_005167118.1	mitogen-activated protein kinase 14A isoform X1
XM_005167061.3	XP_005167118.1	mitogen-activated protein kinase 14A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAPK14	NP_001306.1
Saccharomyces cerevisiae (baker's yeast)	HOG1	NP_013214.1
Neurospora crassa	NCU07024	XP_962163.2
Canis lupus familiaris (dog)	MAPK14	NP_001003206.1
Rattus norvegicus (Norway rat)	Mapk14	NP_112282.2
Kluyveromyces lactis	KLLA0F20053g	XP_455981.1
Eremothecium gossypii	AGOS_AGR048C	NP_986713.1
Bos taurus (cattle)	MAPK14	NP_001095644.1
Drosophila melanogaster (fruit fly)	Mpk2	NP_001163711.1
Caenorhabditis elegans (roundworm)	pmk-1	NP_501365.1
Magnaporthe oryzae (rice blast fungus)	MGG_01822	XP_003714838.1
Oryza sativa (rice)	Os03g0285800	NP_001049770.1
Xenopus tropicalis (tropical clawed frog)	mapk14	NP_001005824.1
	LOC718976	XP_001112423.2
Mus musculus (house mouse)	Mapk14	NP_001161980.1
Gallus gallus (chicken)	MAPK14	XP_001232616.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012148	XP_320380.4
Schizosaccharomyces pombe (fission yeast)	sty1	NP_592843.1
Pan troglodytes (chimpanzee)	MAPK14	NP_001009065.1
Danio rerio (zebrafish)	mapk14a	NP_571797.1
Drosophila melanogaster (fruit fly)	p38b	NP_477361.1
Arabidopsis thaliana (thale cress)	MPK3	NP_190150.1

Gene Ontology
Bibliography

Related articles in PubMed

Irf8 regulates the progression of myeloproliferative neoplasm-like syndrome via Mertk signaling in zebrafish.
Zhao F, Shi Y, Huang Y, Zhan Y, Zhou L, Li Y, Wan Y, Li H, Huang H, Ruan H, Luo L, Li L
Leukemia32(1)149-158(2018 Jan)

Sensitive biomarkers identification for differentiating Cd and Pb induced toxicity on zebrafish embryos.
Yin J, Wang AP, Li WF, Shi R, Jin HT, Wei JF
Environmental toxicology and pharmacology56340-349(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

P38 and JNK have opposing effects on persistence of in vivo leukocyte migration in zebrafish.
Title: P38 and JNK have opposing effects on persistence of in vivo leukocyte migration in zebrafish.

Adult zebrafish cardiomyocytes express active p38alpha MAPK, which is switched off upon entry into mitosis.
Title: p38? MAPK regulates myocardial regeneration in zebrafish.

Dkk3r regulates p38a phosphorylation to maintain Smad4 stability, in turn enabling the Smad2.Smad3a.Smad4 complex to form and activate the myf5 promoter.
Title: dickkopf-3-related gene regulates the expression of zebrafish myf5 gene through phosphorylated p38a-dependent Smad4 activity.

The following MAPK14A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPK14A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01907	NM_131722.1 Latest version!	Danio rerio mitogen-activated protein kinase 14a (mapk14a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa14240	XM_005167061.4 Latest version!	Danio rerio mitogen-activated protein kinase 14a (mapk14a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa14240	XM_005167061.3	Danio rerio mitogen-activated protein kinase 14a (mapk14a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAPK14A ( NM_131722.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571797.1 Danio rerio mitogen-activated protein kinase 14a (mapk14a), mRNA.

Price & Availability↑

CloneID	ODa01907
Clone ID Related Accession (Same CDS sequence)	NM_131722.1
Accession Version	NM_131722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase 14A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB030897.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB030897.1, BC059417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCAGA AAGAAAGACC CACTTTCTAT CGACAGGAGG TCAACAAGAC GATTTGGGAG 
GTCCCCGTGC AGTATCAGAA CTTGTCTCCC GTGGGCTCCG GTGCATACGG ATCTGTTTGC 
TCTGCGTTTG ATGCAAAGAC AGGATTTAAA GTTGCTGTGA AGAAGCTCTC GAGGCCATTT 
CAGTCCATCA TCCATGCCAA GCGGACCTAC AGGGAGCTGA GGCTACTCAA GCACATGAGA 
CATGAGAATG TGATCGGCTT GCTGGATGTT TTTACACCTG CCACAAGTCT GAAGGAGTTT 
AATGATGTGT ACCTGGTGAC TCACCTTATG GGCGCTGACC TGAATAACAT CGTAAAGTGT 
CAGAAACTGA CCGACGATCA CGTCCAGTTC CTCATTTACC AGATACTGAG AGGACTAAAG 
TACATCCATT CAGCTGACAT AATTCACAGG GATTTGAAAC CCAGTAATCT GGCAGTAAAT 
GAAGACTGTG AACTCAAGAT TCTTGACTTT GGCTTGGCTC GACACACAGA TGATGAGATG 
ACTGGGTATG TGGCCACAAG GTGGTATCGT GCCCCTGAGA TCATGCTCAA CTGGATGCAC 
TACAACGTGA CGGTGGACAT TTGGTCAGTG GGCTGCATCA TGGCTGAGCT GCTGACGGGC 
AGGACTCTGT TCCCTGGCAC CGATCATATA AACCAGCTTC AGCAGATAAT GCGACTTACA 
GGAACTCCTC CTTCCTCTCT AATAAGCAGG ATGCCTAGCC ATGAGGCTCG TACTTACATC 
AGCTCACTTC CTCAGATGCC CAAGAGGAAT TTTGCAGATG TGTTTATCGG GGCAAATCCA 
CAAGCTGTGG ACCTCTTGGA GAAAATGCTG GTTTTGGACA CTGATAAGCG GATTACAGCA 
GCAGAGGCTC TGGCACATCC ATACTTTGCC CAATATCATG ACCCGGATGA TGAACCCGAA 
GCAGAGCCTT TTGACCAGAG CTTTGAGAGC CGAGAGCTGG ATATTGAGGA GTGGAAACGT 
CAGACATACG AGGAGATGAT CAGTTTTGAG CCTCCAGTCT TTGATGTGGA TGAAATGGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571797.1
CDS	241..1326
Translation	MSQKERPTFYRQEVNKTIWEVPVQYQNLSPVGSGAYGSVCSAFDAKTGFKVAVKKLSRPFQSIIHAKRTYRELRLLKHMRHENVIGLLDVFTPATSLKEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNVTVDIWSVGCIMAELLTGRTLFPGTDHINQLQQIMRLTGTPPSSLISRMPSHEARTYISSLPQMPKRNFADVFIGANPQAVDLLEKMLVLDTDKRITAAEALAHPYFAQYHDPDDEPEAEPFDQSFESRELDIEEWKRQTYEEMISFEPPVFDVDEMES

Target ORF information:

RefSeq Version	NM_131722.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase 14a (mapk14a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131722.1

ORF Insert Sequence:

ATGTCGCAGA AAGAAAGACC CACTTTCTAT CGACAGGAGG TCAACAAGAC GATTTGGGAG 
GTCCCCGTGC AGTATCAGAA CTTGTCTCCC GTGGGCTCCG GTGCATACGG ATCTGTTTGC 
TCTGCGTTTG ATGCAAAGAC AGGATTTAAA GTTGCTGTGA AGAAGCTCTC GAGGCCATTT 
CAGTCCATCA TCCATGCCAA GCGGACCTAC AGGGAGCTGA GGCTACTCAA GCACATGAGA 
CATGAGAATG TGATCGGCTT GCTGGATGTT TTTACACCTG CCACAAGTCT GAAGGAGTTT 
AATGATGTGT ACCTGGTGAC TCACCTTATG GGCGCTGACC TGAATAACAT CGTAAAGTGT 
CAGAAACTGA CCGACGATCA CGTCCAGTTC CTCATTTACC AGATACTGAG AGGACTAAAG 
TACATCCATT CAGCTGACAT AATTCACAGG GATTTGAAAC CCAGTAATCT GGCAGTAAAT 
GAAGACTGTG AACTCAAGAT TCTTGACTTT GGCTTGGCTC GACACACAGA TGATGAGATG 
ACTGGGTATG TGGCCACAAG GTGGTATCGT GCCCCTGAGA TCATGCTCAA CTGGATGCAC 
TACAACGTGA CGGTGGACAT TTGGTCAGTG GGCTGCATCA TGGCTGAGCT GCTGACGGGC 
AGGACTCTGT TCCCTGGCAC CGATCATATA AACCAGCTTC AGCAGATAAT GCGACTTACA 
GGAACTCCTC CTTCCTCTCT AATAAGCAGG ATGCCTAGCC ATGAGGCTCG TACTTACATC 
AGCTCACTTC CTCAGATGCC CAAGAGGAAT TTTGCAGATG TGTTTATCGG GGCAAATCCA 
CAAGCTGTGG ACCTCTTGGA GAAAATGCTG GTTTTGGACA CTGATAAGCG GATTACAGCA 
GCAGAGGCTC TGGCACATCC ATACTTTGCC CAATATCATG ACCCGGATGA TGAACCCGAA 
GCAGAGCCTT TTGACCAGAG CTTTGAGAGC CGAGAGCTGG ATATTGAGGA GTGGAAACGT 
CAGACATACG AGGAGATGAT CAGTTTTGAG CCTCCAGTCT TTGATGTGGA TGAAATGGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPK14A ( XM_005167061.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167118.1 Danio rerio mitogen-activated protein kinase 14a (mapk14a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14240
Clone ID Related Accession (Same CDS sequence)	XM_005167061.4 , XM_005167061.3
Accession Version	XM_005167061.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase 14A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167061.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCAGA AAGAAAGACC CACTTTCTAT CGACAGGAGG TCAACAAGAC GATTTGGGAG 
GTCCCCGTGC AGTATCAGAA CTTGTCTCCC GTGGGCTCCG GTGCATACGG ATCTGTTTGC 
TCTGCGTTTG ATGCAAAGAC AGGATTTAAA GTTGCTGTGA AGAAGCTCTC GAGGCCATTT 
CAGTCCATCA TCCATGCCAA GCGGACCTAC AGGGAGCTGA GGCTACTCAA GCACATGAGA 
CATGAGAATG TGATCGGCTT GCTGGATGTT TTTACACCTG CCACAAGTCT GAAGGAGTTT 
AATGATGTGT ACCTGGTGAC TCACCTTATG GGCGCTGACC TGAATAACAT CGTAAAGTGT 
CAGAAACTGA CCGACGATCA CGTCCAGTTC CTCATTTACC AGATACTGAG AGGACTAAAG 
TACATCCATT CAGCTGACAT AATTCACAGG GATTTGAAAC CCAGTAATCT GGCAGTAAAT 
GAAGACTGTG AACTCAAGAT TCTTGACTTT GGCTTGGCTC GACACACAGA TGATGAGATG 
ACTGGGTATG TGGCCACAAG GTGGTATCGT GCCCCTGAGA TCATGCTCAA CTGGATGCAC 
TACAACGTGA CGGTGGACAT TTGGTCAGTG GGCTGCATCA TGGCTGAGCT GCTGACGGGC 
AGGACTCTGT TCCCTGGCAC CGATCACATC GATCAGTTGA AGCTCATTAT GCTTTTGGTT 
GGGACTCCAG GACCCGGGCT GCTAATGAAA ATATCATCAG AGTCTGCTCG TACTTACATC 
AGCTCACTTC CTCAGATGCC CAAGAGGAAT TTTGCAGATG TGTTTATCGG GGCAAATCCA 
CAAGCTGTGG ACCTCTTGGA GAAAATGCTG GTTTTGGACA CTGATAAGCG GATTACAGCA 
GCAGAGGCTC TGGCACATCC ATACTTTGCC CAATATCATG ACCCGGATGA TGAACCCGAA 
GCAGAGCCTT TTGACCAGAG CTTTGAGAGC CGAGAGCTGG ATATTGAGGA GTGGAAACGT 
CAGACATACG AGGAGATGAT CAGTTTTGAG CCTCCAGTCT TTGATGTGGA TGAAATGGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167118.1
CDS	372..1457
Translation	MSQKERPTFYRQEVNKTIWEVPVQYQNLSPVGSGAYGSVCSAFDAKTGFKVAVKKLSRPFQSIIHAKRTYRELRLLKHMRHENVIGLLDVFTPATSLKEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNVTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLIMLLVGTPGPGLLMKISSESARTYISSLPQMPKRNFADVFIGANPQAVDLLEKMLVLDTDKRITAAEALAHPYFAQYHDPDDEPEAEPFDQSFESRELDIEEWKRQTYEEMISFEPPVFDVDEMES

Target ORF information:

RefSeq Version	XM_005167061.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase 14a (mapk14a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167061.4

ORF Insert Sequence:

ATGTCGCAGA AAGAAAGACC CACTTTCTAT CGACAGGAGG TCAACAAGAC GATTTGGGAG 
GTCCCCGTGC AGTATCAGAA CTTGTCTCCC GTGGGCTCCG GTGCATACGG ATCTGTTTGC 
TCTGCGTTTG ATGCAAAGAC AGGATTTAAA GTTGCTGTGA AGAAGCTCTC GAGGCCATTT 
CAGTCCATCA TCCATGCCAA GCGGACCTAC AGGGAGCTGA GGCTACTCAA GCACATGAGA 
CATGAGAATG TGATCGGCTT GCTGGATGTT TTTACACCTG CCACAAGTCT GAAGGAGTTT 
AATGATGTGT ACCTGGTGAC TCACCTTATG GGCGCTGACC TGAATAACAT CGTAAAGTGT 
CAGAAACTGA CCGACGATCA CGTCCAGTTC CTCATTTACC AGATACTGAG AGGACTAAAG 
TACATCCATT CAGCTGACAT AATTCACAGG GATTTGAAAC CCAGTAATCT GGCAGTAAAT 
GAAGACTGTG AACTCAAGAT TCTTGACTTT GGCTTGGCTC GACACACAGA TGATGAGATG 
ACTGGGTATG TGGCCACAAG GTGGTATCGT GCCCCTGAGA TCATGCTCAA CTGGATGCAC 
TACAACGTGA CGGTGGACAT TTGGTCAGTG GGCTGCATCA TGGCTGAGCT GCTGACGGGC 
AGGACTCTGT TCCCTGGCAC CGATCACATC GATCAGTTGA AGCTCATTAT GCTTTTGGTT 
GGGACTCCAG GACCCGGGCT GCTAATGAAA ATATCATCAG AGTCTGCTCG TACTTACATC 
AGCTCACTTC CTCAGATGCC CAAGAGGAAT TTTGCAGATG TGTTTATCGG GGCAAATCCA 
CAAGCTGTGG ACCTCTTGGA GAAAATGCTG GTTTTGGACA CTGATAAGCG GATTACAGCA 
GCAGAGGCTC TGGCACATCC ATACTTTGCC CAATATCATG ACCCGGATGA TGAACCCGAA 
GCAGAGCCTT TTGACCAGAG CTTTGAGAGC CGAGAGCTGG ATATTGAGGA GTGGAAACGT 
CAGACATACG AGGAGATGAT CAGTTTTGAG CCTCCAGTCT TTGATGTGGA TGAAATGGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPK14A ( XM_005167061.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167118.1 Danio rerio mitogen-activated protein kinase 14a (mapk14a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14240
Clone ID Related Accession (Same CDS sequence)	XM_005167061.4 , XM_005167061.3
Accession Version	XM_005167061.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase 14A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCAGA AAGAAAGACC CACTTTCTAT CGACAGGAGG TCAACAAGAC GATTTGGGAG 
GTCCCCGTGC AGTATCAGAA CTTGTCTCCC GTGGGCTCCG GTGCATACGG ATCTGTTTGC 
TCTGCGTTTG ATGCAAAGAC AGGATTTAAA GTTGCTGTGA AGAAGCTCTC GAGGCCATTT 
CAGTCCATCA TCCATGCCAA GCGGACCTAC AGGGAGCTGA GGCTACTCAA GCACATGAGA 
CATGAGAATG TGATCGGCTT GCTGGATGTT TTTACACCTG CCACAAGTCT GAAGGAGTTT 
AATGATGTGT ACCTGGTGAC TCACCTTATG GGCGCTGACC TGAATAACAT CGTAAAGTGT 
CAGAAACTGA CCGACGATCA CGTCCAGTTC CTCATTTACC AGATACTGAG AGGACTAAAG 
TACATCCATT CAGCTGACAT AATTCACAGG GATTTGAAAC CCAGTAATCT GGCAGTAAAT 
GAAGACTGTG AACTCAAGAT TCTTGACTTT GGCTTGGCTC GACACACAGA TGATGAGATG 
ACTGGGTATG TGGCCACAAG GTGGTATCGT GCCCCTGAGA TCATGCTCAA CTGGATGCAC 
TACAACGTGA CGGTGGACAT TTGGTCAGTG GGCTGCATCA TGGCTGAGCT GCTGACGGGC 
AGGACTCTGT TCCCTGGCAC CGATCACATC GATCAGTTGA AGCTCATTAT GCTTTTGGTT 
GGGACTCCAG GACCCGGGCT GCTAATGAAA ATATCATCAG AGTCTGCTCG TACTTACATC 
AGCTCACTTC CTCAGATGCC CAAGAGGAAT TTTGCAGATG TGTTTATCGG GGCAAATCCA 
CAAGCTGTGG ACCTCTTGGA GAAAATGCTG GTTTTGGACA CTGATAAGCG GATTACAGCA 
GCAGAGGCTC TGGCACATCC ATACTTTGCC CAATATCATG ACCCGGATGA TGAACCCGAA 
GCAGAGCCTT TTGACCAGAG CTTTGAGAGC CGAGAGCTGG ATATTGAGGA GTGGAAACGT 
CAGACATACG AGGAGATGAT CAGTTTTGAG CCTCCAGTCT TTGATGTGGA TGAAATGGAG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167118.1
CDS	385..1470
Translation	MSQKERPTFYRQEVNKTIWEVPVQYQNLSPVGSGAYGSVCSAFDAKTGFKVAVKKLSRPFQSIIHAKRTYRELRLLKHMRHENVIGLLDVFTPATSLKEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNVTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLIMLLVGTPGPGLLMKISSESARTYISSLPQMPKRNFADVFIGANPQAVDLLEKMLVLDTDKRITAAEALAHPYFAQYHDPDDEPEAEPFDQSFESRELDIEEWKRQTYEEMISFEPPVFDVDEMES

Target ORF information:

RefSeq Version	XM_005167061.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase 14a (mapk14a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167061.3

ORF Insert Sequence:

ATGTCGCAGA AAGAAAGACC CACTTTCTAT CGACAGGAGG TCAACAAGAC GATTTGGGAG 
GTCCCCGTGC AGTATCAGAA CTTGTCTCCC GTGGGCTCCG GTGCATACGG ATCTGTTTGC 
TCTGCGTTTG ATGCAAAGAC AGGATTTAAA GTTGCTGTGA AGAAGCTCTC GAGGCCATTT 
CAGTCCATCA TCCATGCCAA GCGGACCTAC AGGGAGCTGA GGCTACTCAA GCACATGAGA 
CATGAGAATG TGATCGGCTT GCTGGATGTT TTTACACCTG CCACAAGTCT GAAGGAGTTT 
AATGATGTGT ACCTGGTGAC TCACCTTATG GGCGCTGACC TGAATAACAT CGTAAAGTGT 
CAGAAACTGA CCGACGATCA CGTCCAGTTC CTCATTTACC AGATACTGAG AGGACTAAAG 
TACATCCATT CAGCTGACAT AATTCACAGG GATTTGAAAC CCAGTAATCT GGCAGTAAAT 
GAAGACTGTG AACTCAAGAT TCTTGACTTT GGCTTGGCTC GACACACAGA TGATGAGATG 
ACTGGGTATG TGGCCACAAG GTGGTATCGT GCCCCTGAGA TCATGCTCAA CTGGATGCAC 
TACAACGTGA CGGTGGACAT TTGGTCAGTG GGCTGCATCA TGGCTGAGCT GCTGACGGGC 
AGGACTCTGT TCCCTGGCAC CGATCACATC GATCAGTTGA AGCTCATTAT GCTTTTGGTT 
GGGACTCCAG GACCCGGGCT GCTAATGAAA ATATCATCAG AGTCTGCTCG TACTTACATC 
AGCTCACTTC CTCAGATGCC CAAGAGGAAT TTTGCAGATG TGTTTATCGG GGCAAATCCA 
CAAGCTGTGG ACCTCTTGGA GAAAATGCTG GTTTTGGACA CTGATAAGCG GATTACAGCA 
GCAGAGGCTC TGGCACATCC ATACTTTGCC CAATATCATG ACCCGGATGA TGAACCCGAA 
GCAGAGCCTT TTGACCAGAG CTTTGAGAGC CGAGAGCTGG ATATTGAGGA GTGGAAACGT 
CAGACATACG AGGAGATGAT CAGTTTTGAG CCTCCAGTCT TTGATGTGGA TGAAATGGAG 
TCTTGA