mcl1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mcl1a
Entrez Gene ID	58122
Full Name	MCL1, BCL2 family apoptosis regulator a
Synonyms	fb72f02,wu:fb70a08,wu:fb72f02,zfMLP1,zfMcl-1a,zgc:109835
General protein information	Preferred Names MCL1, BCL2 family apoptosis regulator a Names MCL1, BCL2 family apoptosis regulator a fb70a08 myeloid cell leukemia 1a myeloid cell leukemia sequence 1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131599.1	NP_571674.1	MCL1 apoptosis regulator, BCL2 family member a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MCL1	NP_001003016.1
Mus musculus (house mouse)	Mcl1	NP_032588.1
Rattus norvegicus (Norway rat)	Mcl1	NP_068618.2
Gallus gallus (chicken)	MCL1	NP_001244212.1
Danio rerio (zebrafish)	mcl1b	NP_919375.2
	MCL1	XP_001102283.1
Danio rerio (zebrafish)	mcl1a	NP_571674.1
Pan troglodytes (chimpanzee)	MCL1	XP_001169313.2
Bos taurus (cattle)	MCL1	NP_001092676.1
Homo sapiens (human)	MCL1	NP_068779.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs.
Hui SP, Sheng DZ, Sugimoto K, Gonzalez-Rajal A, Nakagawa S, Hesselson D, Kikuchi K
Developmental cell43(6)659-672.e5(2017 Dec)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Predominant Activation of JAK/STAT3 Pathway by Interleukin-6 Is Implicated in Hepatocarcinogenesis.
Jung IH, Choi JH, Chung YY, Lim GL, Park YN, Park SW
Neoplasia (New York, N.Y.)17(7)586-97(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

transgenic overexpression of bcl2-like Bcl-xL-like protein 1 or zfMcl-1a in zebrafish larvae interrupts regulation of the homeostatic balance between cell proliferation and programmed cell death during hepatogenesis and leads to liver hyperplasia
Title: Imbalance in liver homeostasis leading to hyperplasia by overexpressing either one of the Bcl-2-related genes, zfBLP1 and zfMcl-1a.

The following mcl1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcl1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02654	NM_131599.1 Latest version!	Danio rerio MCL1 apoptosis regulator, BCL2 family member a (mcl1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mcl1a ( NM_131599.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571674.1 Danio rerio MCL1 apoptosis regulator, BCL2 family member a (mcl1a), mRNA.

Price & Availability↑

CloneID	ODa02654
Clone ID Related Accession (Same CDS sequence)	NM_131599.1
Accession Version	NM_131599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-12
Organism	Danio rerio(zebrafish)
Product	MCL1 apoptosis regulator, BCL2 family member a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF302805.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF302805.1, BC092707.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTGA GTTTGGATTT TAGGCGAACG GCCACGATGA GCTTCTTCGC GCAGGGCGTT 
CAAACTCCGA CGCTAAAGAC GTGCGTCGAA GACGAACTGG ACGGATACAC TGAGGAGGAG 
GAGGCGCCTC TGAAGCGGCT TAGACCGGGT ACAAACGGCC TGAAGGGGCT GCAGCTGGAC 
GGTCGATTTG TTTCTGCGAC AGACGGATCT CTACCGACCA CCCCAGATCC GGAGGAGCTC 
GACTACGCCG AACTGGAACG CGATACTCGG CAGCTCTTAT TGGATTTTTA CCGCACACAC 
ACGGGAATGT GTCCGGTAGA CCGTAAACTC CATCACGCCA TACCGACGAT GAAGCGAGTG 
GTCGACAATA TTCTCGTGAA GCACCAGATC GCATACAAAG GAATGATCCA GCGTCTTCAG 
CTGGACTCTC AGCCGGCCTC TCTGGACTTC ATCAGATGTA TAGCAAGCAC CATGTTTAAA 
GATGGCGTCA CTAACTGGGG CCGAATCGCG AGTCTGGTGG CGTTCGGGGC CGTGGTTTGC 
TCTCGATTGA AGGAGCTCCA GCAGGATCAG TGTGTGGAGA GGGTGGCTGA GCAGATCTCC 
TCATATCTGA CCTCAGAACA GCAGGACTGG ATCCTCAAAA ACAAGAGCTG GCATGGGTTT 
GTGGAGTTTT TCCACCAGGA GGATGTAGAG TCTGTTGTCC GTCATGGTCT TTTGGCGCTG 
GTCGGATGTG CCGGTATCGG TGCCGGTCTG GCCTTCCTCA TCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571674.1
CDS	9..776
Translation	MALSLDFRRTATMSFFAQGVQTPTLKTCVEDELDGYTEEEEAPLKRLRPGTNGLKGLQLDGRFVSATDGSLPTTPDPEELDYAELERDTRQLLLDFYRTHTGMCPVDRKLHHAIPTMKRVVDNILVKHQIAYKGMIQRLQLDSQPASLDFIRCIASTMFKDGVTNWGRIASLVAFGAVVCSRLKELQQDQCVERVAEQISSYLTSEQQDWILKNKSWHGFVEFFHQEDVESVVRHGLLALVGCAGIGAGLAFLIR

Target ORF information:

RefSeq Version	NM_131599.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MCL1 apoptosis regulator, BCL2 family member a (mcl1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131599.1

ORF Insert Sequence:

ATGGCTTTGA GTTTGGATTT TAGGCGAACG GCCACGATGA GCTTCTTCGC GCAGGGCGTT 
CAAACTCCGA CGCTAAAGAC GTGCGTCGAA GACGAACTGG ACGGATACAC TGAGGAGGAG 
GAGGCGCCTC TGAAGCGGCT TAGACCGGGT ACAAACGGCC TGAAGGGGCT GCAGCTGGAC 
GGTCGATTTG TTTCTGCGAC AGACGGATCT CTACCGACCA CCCCAGATCC GGAGGAGCTC 
GACTACGCCG AACTGGAACG CGATACTCGG CAGCTCTTAT TGGATTTTTA CCGCACACAC 
ACGGGAATGT GTCCGGTAGA CCGTAAACTC CATCACGCCA TACCGACGAT GAAGCGAGTG 
GTCGACAATA TTCTCGTGAA GCACCAGATC GCATACAAAG GAATGATCCA GCGTCTTCAG 
CTGGACTCTC AGCCGGCCTC TCTGGACTTC ATCAGATGTA TAGCAAGCAC CATGTTTAAA 
GATGGCGTCA CTAACTGGGG CCGAATCGCG AGTCTGGTGG CGTTCGGGGC CGTGGTTTGC 
TCTCGATTGA AGGAGCTCCA GCAGGATCAG TGTGTGGAGA GGGTGGCTGA GCAGATCTCC 
TCATATCTGA CCTCAGAACA GCAGGACTGG ATCCTCAAAA ACAAGAGCTG GCATGGGTTT 
GTGGAGTTTT TCCACCAGGA GGATGTAGAG TCTGTTGTCC GTCATGGTCT TTTGGCGCTG 
GTCGGATGTG CCGGTATCGG TGCCGGTCTG GCCTTCCTCA TCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs.
Developmental cell43(6)659-672.e5(2017 Dec)
Hui SP,Sheng DZ,Sugimoto K,Gonzalez-Rajal A,Nakagawa S,Hesselson D,Kikuchi K

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Predominant Activation of JAK/STAT3 Pathway by Interleukin-6 Is Implicated in Hepatocarcinogenesis.
Neoplasia (New York, N.Y.)17(7)586-97(2015 Jul)
Jung IH,Choi JH,Chung YY,Lim GL,Park YN,Park SW