pou4f1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pou4f1
Entrez Gene ID	58057
Full Name	POU class 4 homeobox 1
Synonyms	brn3.0,brn3a
General protein information	Preferred Names POU class 4 homeobox 1 Names POU domain, class 4, transcription factor 1 brain-specific homeobox/POU domain protein 3a brn3b etID22526.1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001312866.1	NP_001299795.1	POU domain, class 4, transcription factor 1
NM_001312866.2	NP_001299795.1	POU domain, class 4, transcription factor 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pou4f1	NP_958442.1
Mus musculus (house mouse)	Pou4f1	NP_035273.3
Homo sapiens (human)	POU4F1	NP_006228.3
Pan troglodytes (chimpanzee)	POU4F1	XP_003314220.1
Rattus norvegicus (Norway rat)	Pou4f1	XP_006252496.1
Xenopus tropicalis (tropical clawed frog)	LOC100488759	XP_002931972.1

Gene Ontology
Bibliography

Related articles in PubMed

Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart.
Maskell LJ, Qamar K, Babakr AA, Hawkins TA, Heads RJ, Budhram-Mahadeo VS
Cell death & disease8(6)e2861(2017 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Habenular Neurogenesis in Zebrafish Is Regulated by a Hedgehog, Pax6 Proneural Gene Cascade.
Halluin C, Madelaine R, Naye F, Peers B, Roussign? M, Blader P
PloS one11(7)e0158210(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pou4f1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pou4f1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa39360	NM_001312866.2 Latest version!	Danio rerio POU class 4 homeobox 1 (pou4f1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
ODa39360	NM_001312866.1	Danio rerio POU class 4 homeobox 1 (pou4f1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pou4f1 ( NM_001312866.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001299795.1 Danio rerio POU class 4 homeobox 1 (pou4f1), mRNA.

Price & Availability↑

CloneID	ODa39360
Clone ID Related Accession (Same CDS sequence)	NM_001312866.1 , NM_001312866.2
Accession Version	NM_001312866.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-26
Organism	Danio rerio(Zebrafish)
Product	POU domain, class 4, transcription factor 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01046894.1, CK141066.1, BC056326.1 and BC065954.1. On Aug 19, 2015 this sequence version replaced XM_005165689.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065954.1, BC056326.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTCCA TGAACAGCAA ACAGCCTCAT TTCGCCATGC ACCCCAGCTT ACCTGAGCAC 
AAGTACACAA CTCTGCACTC GAGCTCGGAG GCGATCAGGA GAGCCTGTCT ACAAACTCCA 
CAGCTGCAGA GCAACATCTT CGCCAGTCTG GATGAGACGC TCCTGGCCCG CGCCGAAGCT 
CTGGCGGCCG TGGACATCGC GGTGTCCCAG GGTAAGAGTC ACCCGTTCAA GCCCGACGCC 
ACGTACCACA CGATGAACAC CGTGCCATGC TCGTCCACAT CCACCGTGCC ACTCGCCCAC 
CACCACCACC ACCATCATCA CCACCACCAC CAGAACCTGG AGCCGGCCGA CCTCATGGAG 
CACATCACCT CGCCGTCGCT CGCGCTCATG CCCAGCGCGC ACGAAGGGGC CGGCGGAGGT 
GGCGGCGGGG GCGGCGGCGG GGGCTTGATC TCCACGTCGG CCCATCCGCA CTCGCACATG 
CACGGGCTCA CCCACCTGTC CCACCAGGCT ATGAACATGA ACTCGCCGCT CTCTCACCAT 
GGGCTCTTGC CGGGCCATCA CGGCGGTGCG CACCAAGGTG CGCCGGGACT CGTCAACAAC 
GGACTGCCCT CAATTAACGA CTCGGACACG GACCCGAGGG AGCTGGAGGC GTTCGCGGAG 
CGCTTCAAAC AGCGGAGGAT CAAACTCGGG GTGACTCAGG CGGACGTGGG GGGCGCGCTG 
GCCAACCTGA AGATCCCAGG CGTGGGCTCG CTGAGCCAAA GTACCATTTG TCGCTTCGAG 
TCGCTAACTC TGTCGCACAA CAACATGATC GCCCTCAAAC CCATCCTCCA GGCGTGGCTG 
GAGGAGGCCG AGGGCGCCCA GCGCGAGAAA ATGAGCAAAC CCGACATTTT CAACGGCAGC 
GAGAAGAAGC GCAAGCGGAC CTCCATAGCG GCCCCGGAGA AACGATCTCT GGAGGCGTAT 
TTCGCGGTGC AGCCTCGGCC GTCCTCGGAG AAAATCGCGG CTATAGCTGA GAAATTGGAC 
CTCAAAAAGA ACGTGGTGCG AGTATGGTTT TGCAACCAAA GACAAAAACA GAAGAGGTTG 
AAATTTTCCG CGGCTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299795.1
CDS	360..1460
Translation	MMSMNSKQPHFAMHPSLPEHKYTTLHSSSEAIRRACLQTPQLQSNIFASLDETLLARAEALAAVDIAVSQGKSHPFKPDATYHTMNTVPCSSTSTVPLAHHHHHHHHHHHQNLEPADLMEHITSPSLALMPSAHEGAGGGGGGGGGGGLISTSAHPHSHMHGLTHLSHQAMNMNSPLSHHGLLPGHHGGAHQGAPGLVNNGLPSINDSDTDPRELEAFAERFKQRRIKLGVTQADVGGALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMSKPDIFNGSEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRLKFSAAH

Target ORF information:

RefSeq Version	NM_001312866.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio POU class 4 homeobox 1 (pou4f1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312866.1

ORF Insert Sequence:

ATGATGTCCA TGAACAGCAA ACAGCCTCAT TTCGCCATGC ACCCCAGCTT ACCTGAGCAC 
AAGTACACAA CTCTGCACTC GAGCTCGGAG GCGATCAGGA GAGCCTGTCT ACAAACTCCA 
CAGCTGCAGA GCAACATCTT CGCCAGTCTG GATGAGACGC TCCTGGCCCG CGCCGAAGCT 
CTGGCGGCCG TGGACATCGC GGTGTCCCAG GGTAAGAGTC ACCCGTTCAA GCCCGACGCC 
ACGTACCACA CGATGAACAC CGTGCCATGC TCGTCCACAT CCACCGTGCC ACTCGCCCAC 
CACCACCACC ACCATCATCA CCACCACCAC CAGAACCTGG AGCCGGCCGA CCTCATGGAG 
CACATCACCT CGCCGTCGCT CGCGCTCATG CCCAGCGCGC ACGAAGGGGC CGGCGGAGGT 
GGCGGCGGGG GCGGCGGCGG GGGCTTGATC TCCACGTCGG CCCATCCGCA CTCGCACATG 
CACGGGCTCA CCCACCTGTC CCACCAGGCT ATGAACATGA ACTCGCCGCT CTCTCACCAT 
GGGCTCTTGC CGGGCCATCA CGGCGGTGCG CACCAAGGTG CGCCGGGACT CGTCAACAAC 
GGACTGCCCT CAATTAACGA CTCGGACACG GACCCGAGGG AGCTGGAGGC GTTCGCGGAG 
CGCTTCAAAC AGCGGAGGAT CAAACTCGGG GTGACTCAGG CGGACGTGGG GGGCGCGCTG 
GCCAACCTGA AGATCCCAGG CGTGGGCTCG CTGAGCCAAA GTACCATTTG TCGCTTCGAG 
TCGCTAACTC TGTCGCACAA CAACATGATC GCCCTCAAAC CCATCCTCCA GGCGTGGCTG 
GAGGAGGCCG AGGGCGCCCA GCGCGAGAAA ATGAGCAAAC CCGACATTTT CAACGGCAGC 
GAGAAGAAGC GCAAGCGGAC CTCCATAGCG GCCCCGGAGA AACGATCTCT GGAGGCGTAT 
TTCGCGGTGC AGCCTCGGCC GTCCTCGGAG AAAATCGCGG CTATAGCTGA GAAATTGGAC 
CTCAAAAAGA ACGTGGTGCG AGTATGGTTT TGCAACCAAA GACAAAAACA GAAGAGGTTG 
AAATTTTCCG CGGCTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pou4f1 ( NM_001312866.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001299795.1 Danio rerio POU class 4 homeobox 1 (pou4f1), mRNA.

Price & Availability↑

CloneID	ODa39360
Clone ID Related Accession (Same CDS sequence)	NM_001312866.1 , NM_001312866.2
Accession Version	NM_001312866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-12
Organism	Danio rerio(zebrafish)
Product	POU domain, class 4, transcription factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01046894.1 and EH590787.1. On Jul 25, 2017 this sequence version replaced NM_001312866.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB188114.1, GFIL01030990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTCCA TGAACAGCAA ACAGCCTCAT TTCGCCATGC ACCCCAGCTT ACCTGAGCAC 
AAGTACACAA CTCTGCACTC GAGCTCGGAG GCGATCAGGA GAGCCTGTCT ACAAACTCCA 
CAGCTGCAGA GCAACATCTT CGCCAGTCTG GATGAGACGC TCCTGGCCCG CGCCGAAGCT 
CTGGCGGCCG TGGACATCGC GGTGTCCCAG GGTAAGAGTC ACCCGTTCAA GCCCGACGCC 
ACGTACCACA CGATGAACAC CGTGCCATGC TCGTCCACAT CCACCGTGCC ACTCGCCCAC 
CACCACCACC ACCATCATCA CCACCACCAC CAGAACCTGG AGCCGGCCGA CCTCATGGAG 
CACATCACCT CGCCGTCGCT CGCGCTCATG CCCAGCGCGC ACGAAGGGGC CGGCGGAGGT 
GGCGGCGGGG GCGGCGGCGG GGGCTTGATC TCCACGTCGG CCCATCCGCA CTCGCACATG 
CACGGGCTCA CCCACCTGTC CCACCAGGCT ATGAACATGA ACTCGCCGCT CTCTCACCAT 
GGGCTCTTGC CGGGCCATCA CGGCGGTGCG CACCAAGGTG CGCCGGGACT CGTCAACAAC 
GGACTGCCCT CAATTAACGA CTCGGACACG GACCCGAGGG AGCTGGAGGC GTTCGCGGAG 
CGCTTCAAAC AGCGGAGGAT CAAACTCGGG GTGACTCAGG CGGACGTGGG GGGCGCGCTG 
GCCAACCTGA AGATCCCAGG CGTGGGCTCG CTGAGCCAAA GTACCATTTG TCGCTTCGAG 
TCGCTAACTC TGTCGCACAA CAACATGATC GCCCTCAAAC CCATCCTCCA GGCGTGGCTG 
GAGGAGGCCG AGGGCGCCCA GCGCGAGAAA ATGAGCAAAC CCGACATTTT CAACGGCAGC 
GAGAAGAAGC GCAAGCGGAC CTCCATAGCG GCCCCGGAGA AACGATCTCT GGAGGCGTAT 
TTCGCGGTGC AGCCTCGGCC GTCCTCGGAG AAAATCGCGG CTATAGCTGA GAAATTGGAC 
CTCAAAAAGA ACGTGGTGCG AGTATGGTTT TGCAACCAAA GACAAAAACA GAAGAGGTTG 
AAATTTTCCG CGGCTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299795.1
CDS	360..1460
Translation	MMSMNSKQPHFAMHPSLPEHKYTTLHSSSEAIRRACLQTPQLQSNIFASLDETLLARAEALAAVDIAVSQGKSHPFKPDATYHTMNTVPCSSTSTVPLAHHHHHHHHHHHQNLEPADLMEHITSPSLALMPSAHEGAGGGGGGGGGGGLISTSAHPHSHMHGLTHLSHQAMNMNSPLSHHGLLPGHHGGAHQGAPGLVNNGLPSINDSDTDPRELEAFAERFKQRRIKLGVTQADVGGALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMSKPDIFNGSEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRLKFSAAH

Target ORF information:

RefSeq Version	NM_001312866.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio POU class 4 homeobox 1 (pou4f1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312866.2

ORF Insert Sequence:

ATGATGTCCA TGAACAGCAA ACAGCCTCAT TTCGCCATGC ACCCCAGCTT ACCTGAGCAC 
AAGTACACAA CTCTGCACTC GAGCTCGGAG GCGATCAGGA GAGCCTGTCT ACAAACTCCA 
CAGCTGCAGA GCAACATCTT CGCCAGTCTG GATGAGACGC TCCTGGCCCG CGCCGAAGCT 
CTGGCGGCCG TGGACATCGC GGTGTCCCAG GGTAAGAGTC ACCCGTTCAA GCCCGACGCC 
ACGTACCACA CGATGAACAC CGTGCCATGC TCGTCCACAT CCACCGTGCC ACTCGCCCAC 
CACCACCACC ACCATCATCA CCACCACCAC CAGAACCTGG AGCCGGCCGA CCTCATGGAG 
CACATCACCT CGCCGTCGCT CGCGCTCATG CCCAGCGCGC ACGAAGGGGC CGGCGGAGGT 
GGCGGCGGGG GCGGCGGCGG GGGCTTGATC TCCACGTCGG CCCATCCGCA CTCGCACATG 
CACGGGCTCA CCCACCTGTC CCACCAGGCT ATGAACATGA ACTCGCCGCT CTCTCACCAT 
GGGCTCTTGC CGGGCCATCA CGGCGGTGCG CACCAAGGTG CGCCGGGACT CGTCAACAAC 
GGACTGCCCT CAATTAACGA CTCGGACACG GACCCGAGGG AGCTGGAGGC GTTCGCGGAG 
CGCTTCAAAC AGCGGAGGAT CAAACTCGGG GTGACTCAGG CGGACGTGGG GGGCGCGCTG 
GCCAACCTGA AGATCCCAGG CGTGGGCTCG CTGAGCCAAA GTACCATTTG TCGCTTCGAG 
TCGCTAACTC TGTCGCACAA CAACATGATC GCCCTCAAAC CCATCCTCCA GGCGTGGCTG 
GAGGAGGCCG AGGGCGCCCA GCGCGAGAAA ATGAGCAAAC CCGACATTTT CAACGGCAGC 
GAGAAGAAGC GCAAGCGGAC CTCCATAGCG GCCCCGGAGA AACGATCTCT GGAGGCGTAT 
TTCGCGGTGC AGCCTCGGCC GTCCTCGGAG AAAATCGCGG CTATAGCTGA GAAATTGGAC 
CTCAAAAAGA ACGTGGTGCG AGTATGGTTT TGCAACCAAA GACAAAAACA GAAGAGGTTG 
AAATTTTCCG CGGCTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart.
Cell death & disease8(6)e2861(2017 Jun)
Maskell LJ,Qamar K,Babakr AA,Hawkins TA,Heads RJ,Budhram-Mahadeo VS

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Habenular Neurogenesis in Zebrafish Is Regulated by a Hedgehog, Pax6 Proneural Gene Cascade.
PloS one11(7)e0158210(2016)
Halluin C,Madelaine R,Naye F,Peers B,Roussign? M,Blader P