alox5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	alox5a
Entrez Gene ID	567204
Full Name	arachidonate 5-lipoxygenase a
Synonyms	alox5,si:dkey-194n13.2
General protein information	Preferred Names arachidonate 5-lipoxygenase a Names arachidonate 5-lipoxygenase ALOX2 LOX2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001303262.1	NP_001290191.1	arachidonate 5-lipoxygenase

Pathways from BioSystems

Homologies

Homo sapiens (human)	ALOX5	NP_000689.1
Oryza sativa (rice)	Os03g0738600	NP_001051212.1
Canis lupus familiaris (dog)	ALOX5	NP_001192042.1
Bos taurus (cattle)	ALOX5	NP_001179721.1
Arabidopsis thaliana (thale cress)	LOX5	NP_188879.2
Pan troglodytes (chimpanzee)	ALOX5	XP_507763.3
	ALOX5	XP_001102354.1
Mus musculus (house mouse)	Alox5	NP_033792.1
Rattus norvegicus (Norway rat)	Alox5	NP_036954.1
Gallus gallus (chicken)	ALOX5	XP_003641560.1
Xenopus tropicalis (tropical clawed frog)	alox5	XP_004915982.1
Oryza sativa (rice)	Os03g0700400	NP_001050993.1
Danio rerio (zebrafish)	alox5a	NP_001243676.1
Arabidopsis thaliana (thale cress)	LOX1	NP_175900.1
Oryza sativa (rice)	Os03g0699700	NP_001050990.1
Oryza sativa (rice)	Os03g0700700	NP_001050995.2

Gene Ontology
Bibliography

Related articles in PubMed

Glucocorticoid-Induced Attenuation of the Inflammatory Response in Zebrafish.
Chatzopoulou A, Heijmans JP, Burgerhout E, Oskam N, Spaink HP, Meijer AH, Schaaf MJ
Endocrinology157(7)2772-84(2016 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Exploration of Stereoselectivity in Embryo-Larvae (Danio rerio) Induced by Chiral PCB149 at the Bioconcentration and Gene Expression Levels.
Chai T, Cui F, Mu X, Yang Y, Wang C, Qiu J
PloS one11(5)e0155263(2016)

Enantio-alteration of gene transcription associated with bioconcentration in adult zebrafish (Danio rerio) exposed to chiral PCB149.
Chai T, Cui F, Mu P, Yang Y, Xu N, Yin Z, Jia Q, Yang S, Qiu J, Wang C
Scientific reports619478(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alox5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alox5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa18597	NM_001303262.1 Latest version!	Danio rerio arachidonate 5-lipoxygenase a (alox5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alox5a ( NM_001303262.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001290191.1 Danio rerio arachidonate 5-lipoxygenase a (alox5a), mRNA.

Price & Availability↑

CloneID	ODa18597
Clone ID Related Accession (Same CDS sequence)	NM_001303262.1
Accession Version	NM_001303262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Danio rerio(zebrafish)
Product	arachidonate 5-lipoxygenase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX927098.24 and CU012047.7. On Dec 24, 2014 this sequence version replaced XM_005156644.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GDQH01004857.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGCCCAGCT ACACGGTGAC TGTTGCCACT GGCACTCAGT GGTTTGCTGG CACCGATGAT 
TATATTTATC TGACTCTGGT GGGCTCTGAG GGCTGCAGTG ACAGGACCCT GCTGGATAAA 
CCCCTCTACA ACGACTTCGA GAGAGGAGCG GTGGACTCAT ATGACATCAG CGTAGGAGAG 
GATCTTGGTG AAATTCAGCT GGTTAAAATT GAAAAGAAGA AATACTGGAT GCACGACGAC 
TGGTACTGTA AATACATCAC GGTGAAGACA CCCTATGGAG ATTATGTGGA GTTCCCCTGC 
TTCCGCTGGT TGGTTGATGA CAAAGAGGTG ATCCTCAGAG ATGGCAGGGC TCGATTGCCA 
CAGCACGACA AGACCGGAGT GGCGAAACAG CACAGGCGCA AAGAACTGGA ACAGAGACAG 
AAGACATACA GATGGAAAGA ATGGCATCCT GGCTTCCCGA TGAGTATCGA CTGCAAAGCC 
CATAAAGAGC TTCCACGGGA CATTCAGTTT GACAGCGAGA AAGGAGTGGA CTTTGTGCTT 
AACTACAGCA AAGCGATCGA GAATCTGTAT GTGAACCAGT TCATGCACAT GTTCCAGTCC 
TCATGGGGAG ATTTCGCAGA CTTTGAGCGG ATATTTGTGA GAATTAAGAA CACAATATCA 
GAATATGTGA TGGAGCACTG GAAAGAAGAC TTCATGTTTG GATATCAATA CTTAAACGGC 
TGTAATCCAG TGGTCATCAA AAAATGCACT GAAATTCCTG ACAAGTTTCC AGTCACACAT 
AACATTGTGG AAGACAGTCT GGAGAGGGGT CTCACACTGG AAGAGGAGCT CAAGGAAGGA 
AACATCTTTA TTGCTGACTA TGAGATAATG GATGGTGTCA CCGCCAATTC TACAGACCCT 
TGCACCCTTC AGTACTTGGC GGCACCCATG TGTCTGCTAT ATAAGAACAG TCAGAACAAA 
ATAATGCCGA TTGCCATCCA GCTGCATCAG AAACCAGGAG AGGACAATCC CATCTTCCTT 
CCCAGTGACG ATGAGTATGA CTGGTTGCTG GCCAAAATCT GGGTGAGATC TTCAGACTTT 
CACGTCCATC AGACAGTCAC TCATCTCCTC AGGACGCATC TGATTTCTGA GGTCTTTGGC 
ATAGCCATGT TCAGACAGCT GCCTGCAGTT CATCCTGTTT TTAAGCTGCT GTTACCACAC 
ATCCGTTTCA CCATTGCCAT TAACACCAAG GCTCGTGAGC AGCTCATCTG TGAGTGTGGA 
CTCTTTGACA AGGCTAATGG GACAGGTGGA GGTGGACACA TAGAACTTGT CCAGAGGTCC 
ATGAAGGTCT TCACGTACAA GTCCTTGTGT TTCCCAGAGA ATATAAAAGC ACGAGGCATG 
GACAGCCAGG AGGAACTGCC ATACTACTTC TACAGAGATG ATGGCTGCGC GGTCTGGGAG 
ACTGTGAAAA GTTTTGTGTC CGATGTTGTT GACATCTACT ATGACAAAGA TGAAACGGTG 
CAGGAGGATG AAGAGATCCA GGCGTTTGTC AAAGATGTTT GCAGCTTTGG AATGCAGGAT 
TTGGACCATT GTGAATTTCC CAAGAGTTTG CAAACCAAAG AGGAACTGGT GGAGTATCTA 
ACTGTCGTCA TCTTTACTGC CTCTGCACAA CACGCTGCAG TAAACTTTGG CCAGTATGAC 
TGGTGTTCAT GGATCCCAAA CTCCCCACCA ACCATGCGGA AACCTCCTCC TATGAAGAAA 
GGCGAGGTGG ACTTACAGTA TATAGTGGAG AGTCTGCCTG ACCGAGGACG CTCCTGTTGG 
CATCTGGGAG CTGTTTGGGC CCTCAGTCAG TTTCAAGAAA ATGAGTTGTT CCTAGGCATG 
TATCCAGATG AACACTTTAT TGAGAAACCG GTGAAAGAGG TCATGGTGAA ATTTGGCAAG 
AAACTTGCAG AAGTGAGCAA AACCATCAAG AATCGAAATG AGGGAAAGAA GCTGCCTTAC 
TACTATCTGT CTCCAGACAG AATCCCAAAC AGTGTGGCTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290191.1
CDS	15..2039
Translation	MPSYTVTVATGTQWFAGTDDYIYLTLVGSEGCSDRTLLDKPLYNDFERGAVDSYDISVGEDLGEIQLVKIEKKKYWMHDDWYCKYITVKTPYGDYVEFPCFRWLVDDKEVILRDGRARLPQHDKTGVAKQHRRKELEQRQKTYRWKEWHPGFPMSIDCKAHKELPRDIQFDSEKGVDFVLNYSKAIENLYVNQFMHMFQSSWGDFADFERIFVRIKNTISEYVMEHWKEDFMFGYQYLNGCNPVVIKKCTEIPDKFPVTHNIVEDSLERGLTLEEELKEGNIFIADYEIMDGVTANSTDPCTLQYLAAPMCLLYKNSQNKIMPIAIQLHQKPGEDNPIFLPSDDEYDWLLAKIWVRSSDFHVHQTVTHLLRTHLISEVFGIAMFRQLPAVHPVFKLLLPHIRFTIAINTKAREQLICECGLFDKANGTGGGGHIELVQRSMKVFTYKSLCFPENIKARGMDSQEELPYYFYRDDGCAVWETVKSFVSDVVDIYYDKDETVQEDEEIQAFVKDVCSFGMQDLDHCEFPKSLQTKEELVEYLTVVIFTASAQHAAVNFGQYDWCSWIPNSPPTMRKPPPMKKGEVDLQYIVESLPDRGRSCWHLGAVWALSQFQENELFLGMYPDEHFIEKPVKEVMVKFGKKLAEVSKTIKNRNEGKKLPYYYLSPDRIPNSVAV

Target ORF information:

RefSeq Version	NM_001303262.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio arachidonate 5-lipoxygenase a (alox5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303262.1

ORF Insert Sequence:

ATGCCCAGCT ACACGGTGAC TGTTGCCACT GGCACTCAGT GGTTTGCTGG CACCGATGAT 
TATATTTATC TGACTCTGGT GGGCTCTGAG GGCTGCAGTG ACAGGACCCT GCTGGATAAA 
CCCCTCTACA ACGACTTCGA GAGAGGAGCG GTGGACTCAT ATGACATCAG CGTAGGAGAG 
GATCTTGGTG AAATTCAGCT GGTTAAAATT GAAAAGAAGA AATACTGGAT GCACGACGAC 
TGGTACTGTA AATACATCAC GGTGAAGACA CCCTATGGAG ATTATGTGGA GTTCCCCTGC 
TTCCGCTGGT TGGTTGATGA CAAAGAGGTG ATCCTCAGAG ATGGCAGGGC TCGATTGCCA 
CAGCACGACA AGACCGGAGT GGCGAAACAG CACAGGCGCA AAGAACTGGA ACAGAGACAG 
AAGACATACA GATGGAAAGA ATGGCATCCT GGCTTCCCGA TGAGTATCGA CTGCAAAGCC 
CATAAAGAGC TTCCACGGGA CATTCAGTTT GACAGCGAGA AAGGAGTGGA CTTTGTGCTT 
AACTACAGCA AAGCGATCGA GAATCTGTAT GTGAACCAGT TCATGCACAT GTTCCAGTCC 
TCATGGGGAG ATTTCGCAGA CTTTGAGCGG ATATTTGTGA GAATTAAGAA CACAATATCA 
GAATATGTGA TGGAGCACTG GAAAGAAGAC TTCATGTTTG GATATCAATA CTTAAACGGC 
TGTAATCCAG TGGTCATCAA AAAATGCACT GAAATTCCTG ACAAGTTTCC AGTCACACAT 
AACATTGTGG AAGACAGTCT GGAGAGGGGT CTCACACTGG AAGAGGAGCT CAAGGAAGGA 
AACATCTTTA TTGCTGACTA TGAGATAATG GATGGTGTCA CCGCCAATTC TACAGACCCT 
TGCACCCTTC AGTACTTGGC GGCACCCATG TGTCTGCTAT ATAAGAACAG TCAGAACAAA 
ATAATGCCGA TTGCCATCCA GCTGCATCAG AAACCAGGAG AGGACAATCC CATCTTCCTT 
CCCAGTGACG ATGAGTATGA CTGGTTGCTG GCCAAAATCT GGGTGAGATC TTCAGACTTT 
CACGTCCATC AGACAGTCAC TCATCTCCTC AGGACGCATC TGATTTCTGA GGTCTTTGGC 
ATAGCCATGT TCAGACAGCT GCCTGCAGTT CATCCTGTTT TTAAGCTGCT GTTACCACAC 
ATCCGTTTCA CCATTGCCAT TAACACCAAG GCTCGTGAGC AGCTCATCTG TGAGTGTGGA 
CTCTTTGACA AGGCTAATGG GACAGGTGGA GGTGGACACA TAGAACTTGT CCAGAGGTCC 
ATGAAGGTCT TCACGTACAA GTCCTTGTGT TTCCCAGAGA ATATAAAAGC ACGAGGCATG 
GACAGCCAGG AGGAACTGCC ATACTACTTC TACAGAGATG ATGGCTGCGC GGTCTGGGAG 
ACTGTGAAAA GTTTTGTGTC CGATGTTGTT GACATCTACT ATGACAAAGA TGAAACGGTG 
CAGGAGGATG AAGAGATCCA GGCGTTTGTC AAAGATGTTT GCAGCTTTGG AATGCAGGAT 
TTGGACCATT GTGAATTTCC CAAGAGTTTG CAAACCAAAG AGGAACTGGT GGAGTATCTA 
ACTGTCGTCA TCTTTACTGC CTCTGCACAA CACGCTGCAG TAAACTTTGG CCAGTATGAC 
TGGTGTTCAT GGATCCCAAA CTCCCCACCA ACCATGCGGA AACCTCCTCC TATGAAGAAA 
GGCGAGGTGG ACTTACAGTA TATAGTGGAG AGTCTGCCTG ACCGAGGACG CTCCTGTTGG 
CATCTGGGAG CTGTTTGGGC CCTCAGTCAG TTTCAAGAAA ATGAGTTGTT CCTAGGCATG 
TATCCAGATG AACACTTTAT TGAGAAACCG GTGAAAGAGG TCATGGTGAA ATTTGGCAAG 
AAACTTGCAG AAGTGAGCAA AACCATCAAG AATCGAAATG AGGGAAAGAA GCTGCCTTAC 
TACTATCTGT CTCCAGACAG AATCCCAAAC AGTGTGGCTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glucocorticoid-Induced Attenuation of the Inflammatory Response in Zebrafish.
Endocrinology157(7)2772-84(2016 Jul)
Chatzopoulou A,Heijmans JP,Burgerhout E,Oskam N,Spaink HP,Meijer AH,Schaaf MJ

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Exploration of Stereoselectivity in Embryo-Larvae (Danio rerio) Induced by Chiral PCB149 at the Bioconcentration and Gene Expression Levels.
PloS one11(5)e0155263(2016)
Chai T,Cui F,Mu X,Yang Y,Wang C,Qiu J

Enantio-alteration of gene transcription associated with bioconcentration in adult zebrafish (Danio rerio) exposed to chiral PCB149.
Scientific reports619478(2016 Jan)
Chai T,Cui F,Mu P,Yang Y,Xu N,Yin Z,Jia Q,Yang S,Qiu J,Wang C