vps11 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps11
Entrez Gene ID	565852
Full Name	vacuolar protein sorting 11
Synonyms	zgc:112329
General protein information	Preferred Names vacuolar protein sorting 11 Names vacuolar protein sorting-associated protein 11 homolog unm_w66
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037708.2	NP_001032797.1	vacuolar protein sorting-associated protein 11 homolog

Pathways from BioSystems

Homologies

	VPS11	XP_001101032.1
Canis lupus familiaris (dog)	VPS11	XP_546492.2
Mus musculus (house mouse)	Vps11	NP_082165.1
Homo sapiens (human)	VPS11	NP_068375.3
Xenopus tropicalis (tropical clawed frog)	vps11	NP_001120879.1
Magnaporthe oryzae (rice blast fungus)	MGG_03283	XP_003716678.1
Arabidopsis thaliana (thale cress)	VPS11	NP_027676.1
Pan troglodytes (chimpanzee)	VPS11	XP_508805.2
Neurospora crassa	NCU05422	XP_963245.2
Oryza sativa (rice)	Os04g0382700	NP_001052609.1
Bos taurus (cattle)	VPS11	NP_001069684.1
Rattus norvegicus (Norway rat)	Vps11	NP_001101608.1
Gallus gallus (chicken)	VPS11	XP_417847.2
Danio rerio (zebrafish)	vps11	NP_001032797.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002665	XP_312259.5

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

A Founder Mutation in VPS11 Causes an Autosomal Recessive Leukoencephalopathy Linked to Autophagic Defects.
Zhang J, Lachance V, Schaffner A, Li X, Fedick A, Kaye LE, Liao J, Rosenfeld J, Yachelevich N, Chu ML, Mitchell WG, Boles RG, Moran E, Tokita M, Gorman E, Bagley K, Zhang W, Xia F, Leduc M, Yang Y, Eng C, Wong LJ, Schiffmann R, Diaz GA, Kornreich R, Thummel R, Wasserstein M, Yue Z, Edelmann L
PLoS genetics12(4)e1005848(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Maintenance of melanophore morphology and survival is cathepsin and vps11 dependent in zebrafish.
Clancey LF, Beirl AJ, Linbo TH, Cooper CD
PloS one8(5)e65096(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these data suggest that vps11 promotes normal melanophore morphology and survival by inhibiting cathepsin release and/or activity.
Title: Maintenance of melanophore morphology and survival is cathepsin and vps11 dependent in zebrafish.

Data show that Vps11 is not necessary for normal retinal development or initiation of melanin biosynthesis, but is essential for melanosome maturation and healthy maintenance of the RPE and photoreceptors.
Title: The loss of vacuolar protein sorting 11 (vps11) causes retinal pathogenesis in a vertebrate model of syndromic albinism.

The following vps11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03969	NM_001037708.2 Latest version!	Danio rerio VPS11 core subunit of CORVET and HOPS complexes (vps11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps11 ( NM_001037708.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032797.1 Danio rerio VPS11 core subunit of CORVET and HOPS complexes (vps11), mRNA.

Price & Availability↑

CloneID	ODa03969
Clone ID Related Accession (Same CDS sequence)	NM_001037708.2
Accession Version	NM_001037708.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 11 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098618.1. On Aug 31, 2012 this sequence version replaced NM_001037708.1. ##Evidence-Data-START## Transcript exon combination :: BC098618.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCGT TTTCACAGTG GAGACGATTT GTGTTTTTCG ATAAAGAAAC TGTGAAGGAT 
CCGAGCGAAA ATGGGAAAAA CTTCCAGCTA CCGATGGGCA TATCGGCCTG TGATTCAGGC 
CGAGGACACA TTGTGCTCGG AGATATGGAT GGTCAGATCT GGTTTTTGAC CCGCTCTCTT 
CAGCTATCAA GCTTCCAGGC ATATAAGCTG CGGGTGACGC ACCTGTACCA GCTCAAACAA 
CACAACATCC TGGTGTCAGT GGGGCAGGAT GAACCTGGCA TCAATCCTCT GGTGAAGGTG 
TGGAATCTTG ATAAGAGGGA CAGTGGCAGT CCTCTGTGTA CCCGAATATT CCCTGCCATT 
CCTGGAAACA AACCCACTGA AGTGTCTTGT CTCAGCGTAC ATGAAAACCT TAATTTCATG 
GCTATAGGTT TTACAGATGG CAGTGTTGTG CTGACGAAGG GTGACATCAC CAGAGACCGA 
CACAGCAAAA CGCTCAGCCT CCATGAGGGC AACTGTCCAA TCACAGGCCT GGCTTTCAGA 
CAGGCTGGGA AAGTCACACA CCTGTTTGTT GCCACCTTAG AGAAAGTGCA GTGTTACACG 
TTGTCAGTGA AAGAGTATCC TCGTGTAGAA TTGGACACAC ACGGCTGTGC TCTCCGCTGT 
TCTGCGCTCA CTGACCCGTC ACAGGACTCG CAGTTTATAG TGGCTGGAGA TGATTGTGTG 
TATCTGTACC AGCCTGATGA GAGAGGACCC TGCTTCGCTT TTGATGGGCA TAAGCTTCTG 
ACACACTGGC ACAGAGGATA CCTGCTGCTG CTCACACACA GTAACAAGTC GCCCAGCAAA 
TCTGATTATG GCAGCAGACA GACGTCCCCT ACAGAAAAGC ACATCCTGAC CATTTACGAT 
TTAGACAACA AGTTCATCGC TTACAGTGCG GTATTTGATG ATGTCATTGA CGTTCTGGCT 
GAATGGGGGT CTTTCTACGT TCTAACGAGT GACAGGACGG TGTATATGCT GCAGGAGAAA 
GACACACAGA CCAAGCTGGA GATGCTGTTT AAGAAGAATC TGTTTGTGAT GGCCATTAAC 
TTGGCCAAAA GTCAACACCT GGACAATGAT GGTCTGTCTG AGATATTCAG GCAATATGGA 
GACCATCTTT ACGTCAAAGG AGATCATGAT GGAGCTATTC AACAATATAT CCGCACCATT 
GGGAAGCTCG AACCATCCTA TGTTATCCGC AAGTTTTTGG ATGCACAGAG AATACACAAC 
CTAACCGCAT ACCTGCAGGC CCTTCACAGG CAGTCACTGG CCAACGCTGA TCACACTACA 
CTACTGCTCA ACTGCTACAC CAAACTGAAG GACAGCTCCA AGCTGGAGGA GTTCATAAAG 
AGCAATGAGA GCGAGGTGCA TTTTGATGTT GAAATTGCCA TCAAAGTGTT ACGCCAGGCT 
GGTTATCACA GTCACGCCGT GTTTCTGGCA GAGAGACACA TGCACCATGA ATGGTATCTG 
AAGATCCAGC TAGAGGATTT GAAGAATTAT CAAGAGGCTT TGCGCTACAT CGGTAGGTTG 
CCATTTGATC AGGCTGAGAG TAACATGAAG CGTTATGGTA AAACTCTTAT GCACCATGTT 
CCCGAGAGCT CCACCATCCT CTTAAAGCGC CTCTGCACCG ACTATCACCC CAGCAAAGAC 
TCCACCGACA GGGACAGTCT GGACAGGCCT GTGGAAAACA AGGCCAATCC AGAGGAGTTC 
ATCCCAGTGT TTGCCAATAA CCCGCGAGAG CTGCGATTGT TTCTGGAGCA CATGATCGAG 
GTGGACCCGT TCTCTCCTGA AGGAGTTTAT GACACTCTGC TAGAGCTCAG ACTGCAGGAC 
TGGGCACATG AGCAGGACCC AGGGAAGAAA AAAGTCCTGC AGGAGGCAGC ACTCTCCCTT 
CTGAGGAGCG ACAACACTGT GTTTGACAAG GCCCTCGTGC TCTGCCAGAT GCACAACTTT 
AAAGAAGGAG TGCTGTATTT GTATGAAAAG GGCAAACTGT ATCAGCAGAT CATGCATTAC 
CACATGCAGA ATGAGGAGTA TGGGAAAGTG GTGGAGGCCT GTAAGCGCTA TGGGGACAGC 
GAAGTGTGCT TGTGGGAGCA GGCACTGGGC TATTTCGCAC GCAAAGAAGA AAACTGCAAG 
TCCTACATTA GTGAAGTCCT GAAGCACATC GACGACAATA ACCTGATGCC TCCGCTTCTG 
GTGGTGCAGA CTTTGGCCCA TAATTCCACA GCATCTTTAT CGGTCATCAA AGACTACCTG 
ATTAATAAAC TGGAGAGGGA GACCCAGCAG ATTGAGGAAG ACGAAAGGAA GATTCGCCAG 
TATAGAGAAG AGACGGCTCA TTTACGGTCT GAGATACAAG AACTTAAAAC TTGTGCAAAG 
ATCTTTCAGA AGACCAAGTG CAGCATGTGC AACAGCCCGC TCGAGCTGCC GTCTGTGCAC 
TTCCTGTGTG GCCACTCTTT CCATCAACAC TGCTTAGAGA GCTTCGCTGA GAGTGAGGCA 
GAGTGTCCCA CCTGCACCCC TGAGAACAGG AAGGTCATGG ACATGTTGCG TGCGCAGGAT 
CAGAAACGAG ATCTGCACGA CCATTTTCAC AGACAGTTGA GATGCTCTAA CGACGGCTTC 
TCAGTCATCG CTGACTACTT TGGCCGTGGA GTGTTCAACA AACTCACTCT GATAACGGAT 
CCTCCCGGAG GAAAGGGTGG AGCTGGGAAT CTGGAGGCTG AATTCAACAG AGATCTGCTC 
ATTAACACCA AGAGAAACGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032797.1
CDS	16..2799
Translation	MAAFSQWRRFVFFDKETVKDPSENGKNFQLPMGISACDSGRGHIVLGDMDGQIWFLTRSLQLSSFQAYKLRVTHLYQLKQHNILVSVGQDEPGINPLVKVWNLDKRDSGSPLCTRIFPAIPGNKPTEVSCLSVHENLNFMAIGFTDGSVVLTKGDITRDRHSKTLSLHEGNCPITGLAFRQAGKVTHLFVATLEKVQCYTLSVKEYPRVELDTHGCALRCSALTDPSQDSQFIVAGDDCVYLYQPDERGPCFAFDGHKLLTHWHRGYLLLLTHSNKSPSKSDYGSRQTSPTEKHILTIYDLDNKFIAYSAVFDDVIDVLAEWGSFYVLTSDRTVYMLQEKDTQTKLEMLFKKNLFVMAINLAKSQHLDNDGLSEIFRQYGDHLYVKGDHDGAIQQYIRTIGKLEPSYVIRKFLDAQRIHNLTAYLQALHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKSNESEVHFDVEIAIKVLRQAGYHSHAVFLAERHMHHEWYLKIQLEDLKNYQEALRYIGRLPFDQAESNMKRYGKTLMHHVPESSTILLKRLCTDYHPSKDSTDRDSLDRPVENKANPEEFIPVFANNPRELRLFLEHMIEVDPFSPEGVYDTLLELRLQDWAHEQDPGKKKVLQEAALSLLRSDNTVFDKALVLCQMHNFKEGVLYLYEKGKLYQQIMHYHMQNEEYGKVVEACKRYGDSEVCLWEQALGYFARKEENCKSYISEVLKHIDDNNLMPPLLVVQTLAHNSTASLSVIKDYLINKLERETQQIEEDERKIRQYREETAHLRSEIQELKTCAKIFQKTKCSMCNSPLELPSVHFLCGHSFHQHCLESFAESEAECPTCTPENRKVMDMLRAQDQKRDLHDHFHRQLRCSNDGFSVIADYFGRGVFNKLTLITDPPGGKGGAGNLEAEFNRDLLINTKRNV

Target ORF information:

RefSeq Version	NM_001037708.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio VPS11 core subunit of CORVET and HOPS complexes (vps11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037708.2

ORF Insert Sequence:

ATGGCAGCGT TTTCACAGTG GAGACGATTT GTGTTTTTCG ATAAAGAAAC TGTGAAGGAT 
CCGAGCGAAA ATGGGAAAAA CTTCCAGCTA CCGATGGGCA TATCGGCCTG TGATTCAGGC 
CGAGGACACA TTGTGCTCGG AGATATGGAT GGTCAGATCT GGTTTTTGAC CCGCTCTCTT 
CAGCTATCAA GCTTCCAGGC ATATAAGCTG CGGGTGACGC ACCTGTACCA GCTCAAACAA 
CACAACATCC TGGTGTCAGT GGGGCAGGAT GAACCTGGCA TCAATCCTCT GGTGAAGGTG 
TGGAATCTTG ATAAGAGGGA CAGTGGCAGT CCTCTGTGTA CCCGAATATT CCCTGCCATT 
CCTGGAAACA AACCCACTGA AGTGTCTTGT CTCAGCGTAC ATGAAAACCT TAATTTCATG 
GCTATAGGTT TTACAGATGG CAGTGTTGTG CTGACGAAGG GTGACATCAC CAGAGACCGA 
CACAGCAAAA CGCTCAGCCT CCATGAGGGC AACTGTCCAA TCACAGGCCT GGCTTTCAGA 
CAGGCTGGGA AAGTCACACA CCTGTTTGTT GCCACCTTAG AGAAAGTGCA GTGTTACACG 
TTGTCAGTGA AAGAGTATCC TCGTGTAGAA TTGGACACAC ACGGCTGTGC TCTCCGCTGT 
TCTGCGCTCA CTGACCCGTC ACAGGACTCG CAGTTTATAG TGGCTGGAGA TGATTGTGTG 
TATCTGTACC AGCCTGATGA GAGAGGACCC TGCTTCGCTT TTGATGGGCA TAAGCTTCTG 
ACACACTGGC ACAGAGGATA CCTGCTGCTG CTCACACACA GTAACAAGTC GCCCAGCAAA 
TCTGATTATG GCAGCAGACA GACGTCCCCT ACAGAAAAGC ACATCCTGAC CATTTACGAT 
TTAGACAACA AGTTCATCGC TTACAGTGCG GTATTTGATG ATGTCATTGA CGTTCTGGCT 
GAATGGGGGT CTTTCTACGT TCTAACGAGT GACAGGACGG TGTATATGCT GCAGGAGAAA 
GACACACAGA CCAAGCTGGA GATGCTGTTT AAGAAGAATC TGTTTGTGAT GGCCATTAAC 
TTGGCCAAAA GTCAACACCT GGACAATGAT GGTCTGTCTG AGATATTCAG GCAATATGGA 
GACCATCTTT ACGTCAAAGG AGATCATGAT GGAGCTATTC AACAATATAT CCGCACCATT 
GGGAAGCTCG AACCATCCTA TGTTATCCGC AAGTTTTTGG ATGCACAGAG AATACACAAC 
CTAACCGCAT ACCTGCAGGC CCTTCACAGG CAGTCACTGG CCAACGCTGA TCACACTACA 
CTACTGCTCA ACTGCTACAC CAAACTGAAG GACAGCTCCA AGCTGGAGGA GTTCATAAAG 
AGCAATGAGA GCGAGGTGCA TTTTGATGTT GAAATTGCCA TCAAAGTGTT ACGCCAGGCT 
GGTTATCACA GTCACGCCGT GTTTCTGGCA GAGAGACACA TGCACCATGA ATGGTATCTG 
AAGATCCAGC TAGAGGATTT GAAGAATTAT CAAGAGGCTT TGCGCTACAT CGGTAGGTTG 
CCATTTGATC AGGCTGAGAG TAACATGAAG CGTTATGGTA AAACTCTTAT GCACCATGTT 
CCCGAGAGCT CCACCATCCT CTTAAAGCGC CTCTGCACCG ACTATCACCC CAGCAAAGAC 
TCCACCGACA GGGACAGTCT GGACAGGCCT GTGGAAAACA AGGCCAATCC AGAGGAGTTC 
ATCCCAGTGT TTGCCAATAA CCCGCGAGAG CTGCGATTGT TTCTGGAGCA CATGATCGAG 
GTGGACCCGT TCTCTCCTGA AGGAGTTTAT GACACTCTGC TAGAGCTCAG ACTGCAGGAC 
TGGGCACATG AGCAGGACCC AGGGAAGAAA AAAGTCCTGC AGGAGGCAGC ACTCTCCCTT 
CTGAGGAGCG ACAACACTGT GTTTGACAAG GCCCTCGTGC TCTGCCAGAT GCACAACTTT 
AAAGAAGGAG TGCTGTATTT GTATGAAAAG GGCAAACTGT ATCAGCAGAT CATGCATTAC 
CACATGCAGA ATGAGGAGTA TGGGAAAGTG GTGGAGGCCT GTAAGCGCTA TGGGGACAGC 
GAAGTGTGCT TGTGGGAGCA GGCACTGGGC TATTTCGCAC GCAAAGAAGA AAACTGCAAG 
TCCTACATTA GTGAAGTCCT GAAGCACATC GACGACAATA ACCTGATGCC TCCGCTTCTG 
GTGGTGCAGA CTTTGGCCCA TAATTCCACA GCATCTTTAT CGGTCATCAA AGACTACCTG 
ATTAATAAAC TGGAGAGGGA GACCCAGCAG ATTGAGGAAG ACGAAAGGAA GATTCGCCAG 
TATAGAGAAG AGACGGCTCA TTTACGGTCT GAGATACAAG AACTTAAAAC TTGTGCAAAG 
ATCTTTCAGA AGACCAAGTG CAGCATGTGC AACAGCCCGC TCGAGCTGCC GTCTGTGCAC 
TTCCTGTGTG GCCACTCTTT CCATCAACAC TGCTTAGAGA GCTTCGCTGA GAGTGAGGCA 
GAGTGTCCCA CCTGCACCCC TGAGAACAGG AAGGTCATGG ACATGTTGCG TGCGCAGGAT 
CAGAAACGAG ATCTGCACGA CCATTTTCAC AGACAGTTGA GATGCTCTAA CGACGGCTTC 
TCAGTCATCG CTGACTACTT TGGCCGTGGA GTGTTCAACA AACTCACTCT GATAACGGAT 
CCTCCCGGAG GAAAGGGTGG AGCTGGGAAT CTGGAGGCTG AATTCAACAG AGATCTGCTC 
ATTAACACCA AGAGAAACGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

A Founder Mutation in VPS11 Causes an Autosomal Recessive Leukoencephalopathy Linked to Autophagic Defects.
PLoS genetics12(4)e1005848(2016 Apr)
Zhang J,Lachance V,Schaffner A,Li X,Fedick A,Kaye LE,Liao J,Rosenfeld J,Yachelevich N,Chu ML,Mitchell WG,Boles RG,Moran E,Tokita M,Gorman E,Bagley K,Zhang W,Xia F,Leduc M,Yang Y,Eng C,Wong LJ,Schiffmann R,Diaz GA,Kornreich R,Thummel R,Wasserstein M,Yue Z,Edelmann L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Maintenance of melanophore morphology and survival is cathepsin and vps11 dependent in zebrafish.
PloS one8(5)e65096(2013)
Clancey LF,Beirl AJ,Linbo TH,Cooper CD