MEGF10 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MEGF10
Entrez Gene ID	564571
Full Name	multiple EGF-like-domains 10
Synonyms	fc33e05,wu:fc33e05
General protein information	Preferred Names multiple EGF-like-domains 10 Names multiple epidermal growth factor-like domains protein 10
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001922489.6	XP_001922524.2	multiple epidermal growth factor-like domains protein 10
XM_001922489.5	XP_001922524.2	multiple epidermal growth factor-like domains protein 10

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Megf10	NP_001001979.1
Danio rerio (zebrafish)	megf10	XP_001922524.2
Xenopus tropicalis (tropical clawed frog)	megf10	NP_001072726.1
Pan troglodytes (chimpanzee)	MEGF10	XP_517907.2
Bos taurus (cattle)	MEGF10	NP_001039916.2
	MEGF10	XP_001097941.1
Canis lupus familiaris (dog)	MEGF10	XP_005626533.1
Homo sapiens (human)	MEGF10	NP_115822.1
Rattus norvegicus (Norway rat)	Megf10	NP_001094127.1
Gallus gallus (chicken)	MEGF10	XP_424719.3

Gene Ontology

The following MEGF10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEGF10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15925	XM_001922489.6 Latest version!	Danio rerio multiple EGF-like-domains 10 (megf10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa15925	XM_001922489.5	Danio rerio multiple EGF-like-domains 10 (megf10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEGF10 ( XM_001922489.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001922524.2 Danio rerio multiple EGF-like-domains 10 (megf10), mRNA.

Price & Availability↑

CloneID	ODa15925
Clone ID Related Accession (Same CDS sequence)	XM_001922489.6 , XM_001922489.5
Accession Version	XM_001922489.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	multiple epidermal growth factor-like domains protein 10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922489.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCAT CCTGTGGTCC TCTTCTATTG GCTGTGTCAT GCTGTTTGGT TGCTCTGACA 
TCATCACTCA ACCTGGATGA CCCTAATGTC TGCAGTCACT GGGAAAGTTA CTCAGTGACC 
GTGCAGGAGA CGTACGCTCA TCCGTTTGAT CAAATCTACT ACACCAGCTG CACGGACATC 
CTCAACTGGT TCAAATGCAC TCAGCACAGA GTAAGCTACA GAACGGCCTA TCGCAGGGGT 
GAGAAGACAA TGCATCGACG CAAGTCTCAG TGCTGCCCAG GTTTCTATGA GAGTGGAGAT 
ATATGCGTTC CCCACTGTGC TGAGAAATGT GTGCATGGCC GCTGCGTGGC CCCCAACACC 
TGTCAGTGTG AGCCGGGCTG GGGAGGGGCT GACTGCTCCA GCGCGTGTGA CCGTGATCAC 
TGGGGCCCAC ACTGCAGTAG CCGGTGTCAG TGTAAGAACG AGGCGCTGTG TAACCCCATC 
ACTGGAGCGT GCATATGTGC ACCCGGGTAC CATGGCTGGC GATGTGAAGA CCTCTGTGAC 
CATTCCACTT ATGGCAACAA TTGCCAACAG AAATGTCTGT GCCAGAATAA TGCTACATGC 
CACCACATCA CAGGCGAATG CGTGTGCAGT CCAGGATACA CAGGAGCATT CTGTGAGGAC 
TTGTGCCCTC CGGGAAAACA TGGACAGCAA TGTGAGGAGC GTTGTCCCTG TCAGAACGGT 
GGTGTGTGTC ATCATGTGAC TGGAGAATGT TCCTGTCCTG CTGGTTGGAT GGGAATGGTG 
TGTGGACAAC CCTGTCCGAC GGGACGCTTT GGTAAGAACT GCTCACAGGA GTGTCAGTGT 
CATAATGGAG GAATCTGTTC GCCATCCACA GGACAGTGTG TGTGCAGTTC AGGATACACA 
GGAGAGAGGT GCCAAGATCA GTGCCAGGTT GGCACATATG GTATCGGCTG CAGCCAGGCA 
TGTCGCTGTG TAAATGGCGC TCAGTGCTAT CATGTGAGTG GAGCATGTTT GTGCGAGCAG 
GGATACACCG GAGAAAGCTG TGAGGAGCGC ATTTGTCCGG ATGGTCAATA TGGCCTTAAG 
TGTGACAGGA AGTGCCCATG TAACACCAAC AACACACGCA GTTGTCACCC AATGTCAGGT 
GAGTGTTCCT GCCAGTCGGG CTGGTCGGGG TTGTACTGTA ATGAGACATG TGCTCCTGGT 
TTCTATGGGG AGGCCTGTCA GGAGGTCTGT CGCTGCCAGA ATGGAGCCGA CTGCCACAGT 
GTGAGCGGGG AATGTATCTG TGCACCGGGA TATAAGGGCT CAGACTGTGC CATAGCCTGC 
CCTCCGGGTA CATATGGCAT CAACTGTACT TCTCTGTGTT CTTGTAAAAA TGGAGCCATT 
TGTTCACCCA TCGATGGATC ATGTTCTTGT CAAGCAGGGT GGCATGGGGT GGACTGCTCA 
ATTAACTGCC CCAGTGGAAC GTGGGGTTTG GGCTGTAACC TGTCATGTGT GTGTGGTAAT 
GGAGGCGCAT GCAATGCTCT TGATGGCAAA TGCACATGTA CTCCAGGCTG GAGAGGAGAC 
AGATGTGATC AACATTGTCA GGACGGGACA TATGGATTGG ATTGCCGTGA GCGTTGTGAC 
TGCAGTCATG CAGATGGGTG TCACCCCTCA ACTGGACACT GCCGCTGTCT AGCAGGCTGG 
ACAGGCATTC ATTGTGACAG TGTGTGTGCT GAGGGCCGCT GGGGGCCAAA CTGCTCTCTG 
TCCTGTAACT GTAAAAACAG TGCGTCTTGC TCACCTGATG AAGGTGCATG CGAGTGTGCA 
CCTGGATTCA GAGGAACCAC CTGCCAACAC ATCTGTTCTC CGGGTGTCTT TGGCCACCGC 
TGTAGCCAGG CATGTCCACA CTGTGTCCAC AGCAATGGCC CCTGTCACCA TGTGACCGGC 
CAGTGTGAAT GTCTACCTGG TTTTAAAGGG GCACTCTGCA ACGAAGTGTG TCCCAGTGGC 
AAGTTTGGCA AGAACTGTGG AGGGAGCTGC ACCTGCACTA ATAATGGCAC TTGTAGCCCC 
ATGGACGGCT CATGTCAGTG CTATCCCGGC TGGATTGGCA GTGACTGCTC ACAGCCATGT 
CCTCCGGGTC AGTGGGGTCC CAACTGCATT CACACTTGCA ACTGTCACAA TGGAGCATTC 
TGCAGTGCTT ATGACGGCGA GTGTAAATGT ACGGCCGGCT GGACAGGTCT CTACTGCACA 
CAAAGATGTC CATTGGGTTT CTATGGTAAG GATTGTGTGC AGGCATGTCA ATGTGAGAAT 
GGTGCAGACT GCAATCACAT CTCGGGTCAG TGCACATGCC GTACAGGCTT CATGGGACGT 
CACTGTGAAA CGAAATGTCC TGCGGGTTCA TATGGATATG GCTGCCGGCA AGTTTGTGAT 
TGTCTCAATA ACTCCACCTG TGATCACATG ACTGGCACAT GTTACTGCAA CCCGGGCTGG 
AAAGGCACAC GATGTGATCA GGCTGGAGGG AATATTGCAG AAAGTCCCAA TAGTTTGACA 
AGCGCAGCTC TGCCTATGGA TTCATATCAA ATTGGCGCAA TCACAGGAAT CATCATCCTT 
GTACTTCTTG TGCTCATCCT CCTCCTTCTG TTCATCATTT ACCGCAAAAA ACAGAAAGGC 
AAAGAATCCT CGATGCCTTC GGTCACGTAT ACACCTACTA TGAGGGCCAA CACTGATTAC 
GCCATTGCAG AGTCACTCCC ACAAACAGAG GTCCTTCCCA ACAGTAACTA TTTCTCCAAC 
CCCAGCTACC ACACGCTGAC CCAATGCAGC AGCCCACCTC ACATCAATAA CATACCCTAT 
GGAAAGATGA ACAACAACCA GTTGTTTGTG AACCTGAAGA ACACTGAGCC GAGGAAGCGG 
CTTTCCCTGC TGGATCACAC AGGAACTCTA CCAGCTGACT GGAAGCAAGG CGGATCCTTT 
AGTGAACTCG GAGCTTATGG AGTTGACAGG CGCTACATGG GAAAGTCGCT TCGAGATCTT 
GTGAAGAGTT TGCCCTATCA TGCCAGTTCC TGCTCTCTGA ATAGTTCAGA GAACCCATAC 
GCCACCATCA AAGACCCTCC CCTGCTGTTG ACCAAGAGCA CTGAATGTGG TTATGTGGAA 
ATGAAGTCTC CTGCTCACAG AGATTCACCA TATGCTGAGA TACATTCCTC CAGCCCTGCC 
AACAAAAATG TCTATGAAGT TGAGCCAACC ATCAGTTCTG TCCAGGCACT TACAAACAAC 
AACTGCAACG GGCCATTCTG TCAAGATCCA TATGACTTAC CAAAGAATAG TCACATTCCA 
TGTCATTATG ACCTACTGCC TACAAGAGAC AGCAGCCCAT CACCCACAGA GGACAGCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922524.2
CDS	162..3524
Translation	MMSSCGPLLLAVSCCLVALTSSLNLDDPNVCSHWESYSVTVQETYAHPFDQIYYTSCTDILNWFKCTQHRVSYRTAYRRGEKTMHRRKSQCCPGFYESGDICVPHCAEKCVHGRCVAPNTCQCEPGWGGADCSSACDRDHWGPHCSSRCQCKNEALCNPITGACICAPGYHGWRCEDLCDHSTYGNNCQQKCLCQNNATCHHITGECVCSPGYTGAFCEDLCPPGKHGQQCEERCPCQNGGVCHHVTGECSCPAGWMGMVCGQPCPTGRFGKNCSQECQCHNGGICSPSTGQCVCSSGYTGERCQDQCQVGTYGIGCSQACRCVNGAQCYHVSGACLCEQGYTGESCEERICPDGQYGLKCDRKCPCNTNNTRSCHPMSGECSCQSGWSGLYCNETCAPGFYGEACQEVCRCQNGADCHSVSGECICAPGYKGSDCAIACPPGTYGINCTSLCSCKNGAICSPIDGSCSCQAGWHGVDCSINCPSGTWGLGCNLSCVCGNGGACNALDGKCTCTPGWRGDRCDQHCQDGTYGLDCRERCDCSHADGCHPSTGHCRCLAGWTGIHCDSVCAEGRWGPNCSLSCNCKNSASCSPDEGACECAPGFRGTTCQHICSPGVFGHRCSQACPHCVHSNGPCHHVTGQCECLPGFKGALCNEVCPSGKFGKNCGGSCTCTNNGTCSPMDGSCQCYPGWIGSDCSQPCPPGQWGPNCIHTCNCHNGAFCSAYDGECKCTAGWTGLYCTQRCPLGFYGKDCVQACQCENGADCNHISGQCTCRTGFMGRHCETKCPAGSYGYGCRQVCDCLNNSTCDHMTGTCYCNPGWKGTRCDQAGGNIAESPNSLTSAALPMDSYQIGAITGIIILVLLVLILLLLFIIYRKKQKGKESSMPSVTYTPTMRANTDYAIAESLPQTEVLPNSNYFSNPSYHTLTQCSSPPHINNIPYGKMNNNQLFVNLKNTEPRKRLSLLDHTGTLPADWKQGGSFSELGAYGVDRRYMGKSLRDLVKSLPYHASSCSLNSSENPYATIKDPPLLLTKSTECGYVEMKSPAHRDSPYAEIHSSSPANKNVYEVEPTISSVQALTNNNCNGPFCQDPYDLPKNSHIPCHYDLLPTRDSSPSPTEDSK

Target ORF information:

RefSeq Version	XM_001922489.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio multiple EGF-like-domains 10 (megf10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922489.6

ORF Insert Sequence:

ATGATGTCAT CCTGTGGTCC TCTTCTATTG GCTGTGTCAT GCTGTTTGGT TGCTCTGACA 
TCATCACTCA ACCTGGATGA CCCTAATGTC TGCAGTCACT GGGAAAGTTA CTCAGTGACC 
GTGCAGGAGA CGTACGCTCA TCCGTTTGAT CAAATCTACT ACACCAGCTG CACGGACATC 
CTCAACTGGT TCAAATGCAC TCAGCACAGA GTAAGCTACA GAACGGCCTA TCGCAGGGGT 
GAGAAGACAA TGCATCGACG CAAGTCTCAG TGCTGCCCAG GTTTCTATGA GAGTGGAGAT 
ATATGCGTTC CCCACTGTGC TGAGAAATGT GTGCATGGCC GCTGCGTGGC CCCCAACACC 
TGTCAGTGTG AGCCGGGCTG GGGAGGGGCT GACTGCTCCA GCGCGTGTGA CCGTGATCAC 
TGGGGCCCAC ACTGCAGTAG CCGGTGTCAG TGTAAGAACG AGGCGCTGTG TAACCCCATC 
ACTGGAGCGT GCATATGTGC ACCCGGGTAC CATGGCTGGC GATGTGAAGA CCTCTGTGAC 
CATTCCACTT ATGGCAACAA TTGCCAACAG AAATGTCTGT GCCAGAATAA TGCTACATGC 
CACCACATCA CAGGCGAATG CGTGTGCAGT CCAGGATACA CAGGAGCATT CTGTGAGGAC 
TTGTGCCCTC CGGGAAAACA TGGACAGCAA TGTGAGGAGC GTTGTCCCTG TCAGAACGGT 
GGTGTGTGTC ATCATGTGAC TGGAGAATGT TCCTGTCCTG CTGGTTGGAT GGGAATGGTG 
TGTGGACAAC CCTGTCCGAC GGGACGCTTT GGTAAGAACT GCTCACAGGA GTGTCAGTGT 
CATAATGGAG GAATCTGTTC GCCATCCACA GGACAGTGTG TGTGCAGTTC AGGATACACA 
GGAGAGAGGT GCCAAGATCA GTGCCAGGTT GGCACATATG GTATCGGCTG CAGCCAGGCA 
TGTCGCTGTG TAAATGGCGC TCAGTGCTAT CATGTGAGTG GAGCATGTTT GTGCGAGCAG 
GGATACACCG GAGAAAGCTG TGAGGAGCGC ATTTGTCCGG ATGGTCAATA TGGCCTTAAG 
TGTGACAGGA AGTGCCCATG TAACACCAAC AACACACGCA GTTGTCACCC AATGTCAGGT 
GAGTGTTCCT GCCAGTCGGG CTGGTCGGGG TTGTACTGTA ATGAGACATG TGCTCCTGGT 
TTCTATGGGG AGGCCTGTCA GGAGGTCTGT CGCTGCCAGA ATGGAGCCGA CTGCCACAGT 
GTGAGCGGGG AATGTATCTG TGCACCGGGA TATAAGGGCT CAGACTGTGC CATAGCCTGC 
CCTCCGGGTA CATATGGCAT CAACTGTACT TCTCTGTGTT CTTGTAAAAA TGGAGCCATT 
TGTTCACCCA TCGATGGATC ATGTTCTTGT CAAGCAGGGT GGCATGGGGT GGACTGCTCA 
ATTAACTGCC CCAGTGGAAC GTGGGGTTTG GGCTGTAACC TGTCATGTGT GTGTGGTAAT 
GGAGGCGCAT GCAATGCTCT TGATGGCAAA TGCACATGTA CTCCAGGCTG GAGAGGAGAC 
AGATGTGATC AACATTGTCA GGACGGGACA TATGGATTGG ATTGCCGTGA GCGTTGTGAC 
TGCAGTCATG CAGATGGGTG TCACCCCTCA ACTGGACACT GCCGCTGTCT AGCAGGCTGG 
ACAGGCATTC ATTGTGACAG TGTGTGTGCT GAGGGCCGCT GGGGGCCAAA CTGCTCTCTG 
TCCTGTAACT GTAAAAACAG TGCGTCTTGC TCACCTGATG AAGGTGCATG CGAGTGTGCA 
CCTGGATTCA GAGGAACCAC CTGCCAACAC ATCTGTTCTC CGGGTGTCTT TGGCCACCGC 
TGTAGCCAGG CATGTCCACA CTGTGTCCAC AGCAATGGCC CCTGTCACCA TGTGACCGGC 
CAGTGTGAAT GTCTACCTGG TTTTAAAGGG GCACTCTGCA ACGAAGTGTG TCCCAGTGGC 
AAGTTTGGCA AGAACTGTGG AGGGAGCTGC ACCTGCACTA ATAATGGCAC TTGTAGCCCC 
ATGGACGGCT CATGTCAGTG CTATCCCGGC TGGATTGGCA GTGACTGCTC ACAGCCATGT 
CCTCCGGGTC AGTGGGGTCC CAACTGCATT CACACTTGCA ACTGTCACAA TGGAGCATTC 
TGCAGTGCTT ATGACGGCGA GTGTAAATGT ACGGCCGGCT GGACAGGTCT CTACTGCACA 
CAAAGATGTC CATTGGGTTT CTATGGTAAG GATTGTGTGC AGGCATGTCA ATGTGAGAAT 
GGTGCAGACT GCAATCACAT CTCGGGTCAG TGCACATGCC GTACAGGCTT CATGGGACGT 
CACTGTGAAA CGAAATGTCC TGCGGGTTCA TATGGATATG GCTGCCGGCA AGTTTGTGAT 
TGTCTCAATA ACTCCACCTG TGATCACATG ACTGGCACAT GTTACTGCAA CCCGGGCTGG 
AAAGGCACAC GATGTGATCA GGCTGGAGGG AATATTGCAG AAAGTCCCAA TAGTTTGACA 
AGCGCAGCTC TGCCTATGGA TTCATATCAA ATTGGCGCAA TCACAGGAAT CATCATCCTT 
GTACTTCTTG TGCTCATCCT CCTCCTTCTG TTCATCATTT ACCGCAAAAA ACAGAAAGGC 
AAAGAATCCT CGATGCCTTC GGTCACGTAT ACACCTACTA TGAGGGCCAA CACTGATTAC 
GCCATTGCAG AGTCACTCCC ACAAACAGAG GTCCTTCCCA ACAGTAACTA TTTCTCCAAC 
CCCAGCTACC ACACGCTGAC CCAATGCAGC AGCCCACCTC ACATCAATAA CATACCCTAT 
GGAAAGATGA ACAACAACCA GTTGTTTGTG AACCTGAAGA ACACTGAGCC GAGGAAGCGG 
CTTTCCCTGC TGGATCACAC AGGAACTCTA CCAGCTGACT GGAAGCAAGG CGGATCCTTT 
AGTGAACTCG GAGCTTATGG AGTTGACAGG CGCTACATGG GAAAGTCGCT TCGAGATCTT 
GTGAAGAGTT TGCCCTATCA TGCCAGTTCC TGCTCTCTGA ATAGTTCAGA GAACCCATAC 
GCCACCATCA AAGACCCTCC CCTGCTGTTG ACCAAGAGCA CTGAATGTGG TTATGTGGAA 
ATGAAGTCTC CTGCTCACAG AGATTCACCA TATGCTGAGA TACATTCCTC CAGCCCTGCC 
AACAAAAATG TCTATGAAGT TGAGCCAACC ATCAGTTCTG TCCAGGCACT TACAAACAAC 
AACTGCAACG GGCCATTCTG TCAAGATCCA TATGACTTAC CAAAGAATAG TCACATTCCA 
TGTCATTATG ACCTACTGCC TACAAGAGAC AGCAGCCCAT CACCCACAGA GGACAGCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( XM_001922489.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001922524.2 Danio rerio multiple EGF-like-domains 10 (megf10), mRNA.

Price & Availability↑

CloneID	ODa15925
Clone ID Related Accession (Same CDS sequence)	XM_001922489.6 , XM_001922489.5
Accession Version	XM_001922489.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	multiple epidermal growth factor-like domains protein 10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922489.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCAT CCTGTGGTCC TCTTCTATTG GCTGTGTCAT GCTGTTTGGT TGCTCTGACA 
TCATCACTCA ACCTGGATGA CCCTAATGTC TGCAGTCACT GGGAAAGTTA CTCAGTGACC 
GTGCAGGAGA CGTACGCTCA TCCGTTTGAT CAAATCTACT ACACCAGCTG CACGGACATC 
CTCAACTGGT TCAAATGCAC TCAGCACAGA GTAAGCTACA GAACGGCCTA TCGCAGGGGT 
GAGAAGACAA TGCATCGACG CAAGTCTCAG TGCTGCCCAG GTTTCTATGA GAGTGGAGAT 
ATATGCGTTC CCCACTGTGC TGAGAAATGT GTGCATGGCC GCTGCGTGGC CCCCAACACC 
TGTCAGTGTG AGCCGGGCTG GGGAGGGGCT GACTGCTCCA GCGCGTGTGA CCGTGATCAC 
TGGGGCCCAC ACTGCAGTAG CCGGTGTCAG TGTAAGAACG AGGCGCTGTG TAACCCCATC 
ACTGGAGCGT GCATATGTGC ACCCGGGTAC CATGGCTGGC GATGTGAAGA CCTCTGTGAC 
CATTCCACTT ATGGCAACAA TTGCCAACAG AAATGTCTGT GCCAGAATAA TGCTACATGC 
CACCACATCA CAGGCGAATG CGTGTGCAGT CCAGGATACA CAGGAGCATT CTGTGAGGAC 
TTGTGCCCTC CGGGAAAACA TGGACAGCAA TGTGAGGAGC GTTGTCCCTG TCAGAACGGT 
GGTGTGTGTC ATCATGTGAC TGGAGAATGT TCCTGTCCTG CTGGTTGGAT GGGAATGGTG 
TGTGGACAAC CCTGTCCGAC GGGACGCTTT GGTAAGAACT GCTCACAGGA GTGTCAGTGT 
CATAATGGAG GAATCTGTTC GCCATCCACA GGACAGTGTG TGTGCAGTTC AGGATACACA 
GGAGAGAGGT GCCAAGATCA GTGCCAGGTT GGCACATATG GTATCGGCTG CAGCCAGGCA 
TGTCGCTGTG TAAATGGCGC TCAGTGCTAT CATGTGAGTG GAGCATGTTT GTGCGAGCAG 
GGATACACCG GAGAAAGCTG TGAGGAGCGC ATTTGTCCGG ATGGTCAATA TGGCCTTAAG 
TGTGACAGGA AGTGCCCATG TAACACCAAC AACACACGCA GTTGTCACCC AATGTCAGGT 
GAGTGTTCCT GCCAGTCGGG CTGGTCGGGG TTGTACTGTA ATGAGACATG TGCTCCTGGT 
TTCTATGGGG AGGCCTGTCA GGAGGTCTGT CGCTGCCAGA ATGGAGCCGA CTGCCACAGT 
GTGAGCGGGG AATGTATCTG TGCACCGGGA TATAAGGGCT CAGACTGTGC CATAGCCTGC 
CCTCCGGGTA CATATGGCAT CAACTGTACT TCTCTGTGTT CTTGTAAAAA TGGAGCCATT 
TGTTCACCCA TCGATGGATC ATGTTCTTGT CAAGCAGGGT GGCATGGGGT GGACTGCTCA 
ATTAACTGCC CCAGTGGAAC GTGGGGTTTG GGCTGTAACC TGTCATGTGT GTGTGGTAAT 
GGAGGCGCAT GCAATGCTCT TGATGGCAAA TGCACATGTA CTCCAGGCTG GAGAGGAGAC 
AGATGTGATC AACATTGTCA GGACGGGACA TATGGATTGG ATTGCCGTGA GCGTTGTGAC 
TGCAGTCATG CAGATGGGTG TCACCCCTCA ACTGGACACT GCCGCTGTCT AGCAGGCTGG 
ACAGGCATTC ATTGTGACAG TGTGTGTGCT GAGGGCCGCT GGGGGCCAAA CTGCTCTCTG 
TCCTGTAACT GTAAAAACAG TGCGTCTTGC TCACCTGATG AAGGTGCATG CGAGTGTGCA 
CCTGGATTCA GAGGAACCAC CTGCCAACAC ATCTGTTCTC CGGGTGTCTT TGGCCACCGC 
TGTAGCCAGG CATGTCCACA CTGTGTCCAC AGCAATGGCC CCTGTCACCA TGTGACCGGC 
CAGTGTGAAT GTCTACCTGG TTTTAAAGGG GCACTCTGCA ACGAAGTGTG TCCCAGTGGC 
AAGTTTGGCA AGAACTGTGG AGGGAGCTGC ACCTGCACTA ATAATGGCAC TTGTAGCCCC 
ATGGACGGCT CATGTCAGTG CTATCCCGGC TGGATTGGCA GTGACTGCTC ACAGCCATGT 
CCTCCGGGTC AGTGGGGTCC CAACTGCATT CACACTTGCA ACTGTCACAA TGGAGCATTC 
TGCAGTGCTT ATGACGGCGA GTGTAAATGT ACGGCCGGCT GGACAGGTCT CTACTGCACA 
CAAAGATGTC CATTGGGTTT CTATGGTAAG GATTGTGTGC AGGCATGTCA ATGTGAGAAT 
GGTGCAGACT GCAATCACAT CTCGGGTCAG TGCACATGCC GTACAGGCTT CATGGGACGT 
CACTGTGAAA CGAAATGTCC TGCGGGTTCA TATGGATATG GCTGCCGGCA AGTTTGTGAT 
TGTCTCAATA ACTCCACCTG TGATCACATG ACTGGCACAT GTTACTGCAA CCCGGGCTGG 
AAAGGCACAC GATGTGATCA GGCTGGAGGG AATATTGCAG AAAGTCCCAA TAGTTTGACA 
AGCGCAGCTC TGCCTATGGA TTCATATCAA ATTGGCGCAA TCACAGGAAT CATCATCCTT 
GTACTTCTTG TGCTCATCCT CCTCCTTCTG TTCATCATTT ACCGCAAAAA ACAGAAAGGC 
AAAGAATCCT CGATGCCTTC GGTCACGTAT ACACCTACTA TGAGGGCCAA CACTGATTAC 
GCCATTGCAG AGTCACTCCC ACAAACAGAG GTCCTTCCCA ACAGTAACTA TTTCTCCAAC 
CCCAGCTACC ACACGCTGAC CCAATGCAGC AGCCCACCTC ACATCAATAA CATACCCTAT 
GGAAAGATGA ACAACAACCA GTTGTTTGTG AACCTGAAGA ACACTGAGCC GAGGAAGCGG 
CTTTCCCTGC TGGATCACAC AGGAACTCTA CCAGCTGACT GGAAGCAAGG CGGATCCTTT 
AGTGAACTCG GAGCTTATGG AGTTGACAGG CGCTACATGG GAAAGTCGCT TCGAGATCTT 
GTGAAGAGTT TGCCCTATCA TGCCAGTTCC TGCTCTCTGA ATAGTTCAGA GAACCCATAC 
GCCACCATCA AAGACCCTCC CCTGCTGTTG ACCAAGAGCA CTGAATGTGG TTATGTGGAA 
ATGAAGTCTC CTGCTCACAG AGATTCACCA TATGCTGAGA TACATTCCTC CAGCCCTGCC 
AACAAAAATG TCTATGAAGT TGAGCCAACC ATCAGTTCTG TCCAGGCACT TACAAACAAC 
AACTGCAACG GGCCATTCTG TCAAGATCCA TATGACTTAC CAAAGAATAG TCACATTCCA 
TGTCATTATG ACCTACTGCC TACAAGAGAC AGCAGCCCAT CACCCACAGA GGACAGCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922524.2
CDS	148..3510
Translation	MMSSCGPLLLAVSCCLVALTSSLNLDDPNVCSHWESYSVTVQETYAHPFDQIYYTSCTDILNWFKCTQHRVSYRTAYRRGEKTMHRRKSQCCPGFYESGDICVPHCAEKCVHGRCVAPNTCQCEPGWGGADCSSACDRDHWGPHCSSRCQCKNEALCNPITGACICAPGYHGWRCEDLCDHSTYGNNCQQKCLCQNNATCHHITGECVCSPGYTGAFCEDLCPPGKHGQQCEERCPCQNGGVCHHVTGECSCPAGWMGMVCGQPCPTGRFGKNCSQECQCHNGGICSPSTGQCVCSSGYTGERCQDQCQVGTYGIGCSQACRCVNGAQCYHVSGACLCEQGYTGESCEERICPDGQYGLKCDRKCPCNTNNTRSCHPMSGECSCQSGWSGLYCNETCAPGFYGEACQEVCRCQNGADCHSVSGECICAPGYKGSDCAIACPPGTYGINCTSLCSCKNGAICSPIDGSCSCQAGWHGVDCSINCPSGTWGLGCNLSCVCGNGGACNALDGKCTCTPGWRGDRCDQHCQDGTYGLDCRERCDCSHADGCHPSTGHCRCLAGWTGIHCDSVCAEGRWGPNCSLSCNCKNSASCSPDEGACECAPGFRGTTCQHICSPGVFGHRCSQACPHCVHSNGPCHHVTGQCECLPGFKGALCNEVCPSGKFGKNCGGSCTCTNNGTCSPMDGSCQCYPGWIGSDCSQPCPPGQWGPNCIHTCNCHNGAFCSAYDGECKCTAGWTGLYCTQRCPLGFYGKDCVQACQCENGADCNHISGQCTCRTGFMGRHCETKCPAGSYGYGCRQVCDCLNNSTCDHMTGTCYCNPGWKGTRCDQAGGNIAESPNSLTSAALPMDSYQIGAITGIIILVLLVLILLLLFIIYRKKQKGKESSMPSVTYTPTMRANTDYAIAESLPQTEVLPNSNYFSNPSYHTLTQCSSPPHINNIPYGKMNNNQLFVNLKNTEPRKRLSLLDHTGTLPADWKQGGSFSELGAYGVDRRYMGKSLRDLVKSLPYHASSCSLNSSENPYATIKDPPLLLTKSTECGYVEMKSPAHRDSPYAEIHSSSPANKNVYEVEPTISSVQALTNNNCNGPFCQDPYDLPKNSHIPCHYDLLPTRDSSPSPTEDSK

Target ORF information:

RefSeq Version	XM_001922489.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio multiple EGF-like-domains 10 (megf10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922489.5

ORF Insert Sequence:

ATGATGTCAT CCTGTGGTCC TCTTCTATTG GCTGTGTCAT GCTGTTTGGT TGCTCTGACA 
TCATCACTCA ACCTGGATGA CCCTAATGTC TGCAGTCACT GGGAAAGTTA CTCAGTGACC 
GTGCAGGAGA CGTACGCTCA TCCGTTTGAT CAAATCTACT ACACCAGCTG CACGGACATC 
CTCAACTGGT TCAAATGCAC TCAGCACAGA GTAAGCTACA GAACGGCCTA TCGCAGGGGT 
GAGAAGACAA TGCATCGACG CAAGTCTCAG TGCTGCCCAG GTTTCTATGA GAGTGGAGAT 
ATATGCGTTC CCCACTGTGC TGAGAAATGT GTGCATGGCC GCTGCGTGGC CCCCAACACC 
TGTCAGTGTG AGCCGGGCTG GGGAGGGGCT GACTGCTCCA GCGCGTGTGA CCGTGATCAC 
TGGGGCCCAC ACTGCAGTAG CCGGTGTCAG TGTAAGAACG AGGCGCTGTG TAACCCCATC 
ACTGGAGCGT GCATATGTGC ACCCGGGTAC CATGGCTGGC GATGTGAAGA CCTCTGTGAC 
CATTCCACTT ATGGCAACAA TTGCCAACAG AAATGTCTGT GCCAGAATAA TGCTACATGC 
CACCACATCA CAGGCGAATG CGTGTGCAGT CCAGGATACA CAGGAGCATT CTGTGAGGAC 
TTGTGCCCTC CGGGAAAACA TGGACAGCAA TGTGAGGAGC GTTGTCCCTG TCAGAACGGT 
GGTGTGTGTC ATCATGTGAC TGGAGAATGT TCCTGTCCTG CTGGTTGGAT GGGAATGGTG 
TGTGGACAAC CCTGTCCGAC GGGACGCTTT GGTAAGAACT GCTCACAGGA GTGTCAGTGT 
CATAATGGAG GAATCTGTTC GCCATCCACA GGACAGTGTG TGTGCAGTTC AGGATACACA 
GGAGAGAGGT GCCAAGATCA GTGCCAGGTT GGCACATATG GTATCGGCTG CAGCCAGGCA 
TGTCGCTGTG TAAATGGCGC TCAGTGCTAT CATGTGAGTG GAGCATGTTT GTGCGAGCAG 
GGATACACCG GAGAAAGCTG TGAGGAGCGC ATTTGTCCGG ATGGTCAATA TGGCCTTAAG 
TGTGACAGGA AGTGCCCATG TAACACCAAC AACACACGCA GTTGTCACCC AATGTCAGGT 
GAGTGTTCCT GCCAGTCGGG CTGGTCGGGG TTGTACTGTA ATGAGACATG TGCTCCTGGT 
TTCTATGGGG AGGCCTGTCA GGAGGTCTGT CGCTGCCAGA ATGGAGCCGA CTGCCACAGT 
GTGAGCGGGG AATGTATCTG TGCACCGGGA TATAAGGGCT CAGACTGTGC CATAGCCTGC 
CCTCCGGGTA CATATGGCAT CAACTGTACT TCTCTGTGTT CTTGTAAAAA TGGAGCCATT 
TGTTCACCCA TCGATGGATC ATGTTCTTGT CAAGCAGGGT GGCATGGGGT GGACTGCTCA 
ATTAACTGCC CCAGTGGAAC GTGGGGTTTG GGCTGTAACC TGTCATGTGT GTGTGGTAAT 
GGAGGCGCAT GCAATGCTCT TGATGGCAAA TGCACATGTA CTCCAGGCTG GAGAGGAGAC 
AGATGTGATC AACATTGTCA GGACGGGACA TATGGATTGG ATTGCCGTGA GCGTTGTGAC 
TGCAGTCATG CAGATGGGTG TCACCCCTCA ACTGGACACT GCCGCTGTCT AGCAGGCTGG 
ACAGGCATTC ATTGTGACAG TGTGTGTGCT GAGGGCCGCT GGGGGCCAAA CTGCTCTCTG 
TCCTGTAACT GTAAAAACAG TGCGTCTTGC TCACCTGATG AAGGTGCATG CGAGTGTGCA 
CCTGGATTCA GAGGAACCAC CTGCCAACAC ATCTGTTCTC CGGGTGTCTT TGGCCACCGC 
TGTAGCCAGG CATGTCCACA CTGTGTCCAC AGCAATGGCC CCTGTCACCA TGTGACCGGC 
CAGTGTGAAT GTCTACCTGG TTTTAAAGGG GCACTCTGCA ACGAAGTGTG TCCCAGTGGC 
AAGTTTGGCA AGAACTGTGG AGGGAGCTGC ACCTGCACTA ATAATGGCAC TTGTAGCCCC 
ATGGACGGCT CATGTCAGTG CTATCCCGGC TGGATTGGCA GTGACTGCTC ACAGCCATGT 
CCTCCGGGTC AGTGGGGTCC CAACTGCATT CACACTTGCA ACTGTCACAA TGGAGCATTC 
TGCAGTGCTT ATGACGGCGA GTGTAAATGT ACGGCCGGCT GGACAGGTCT CTACTGCACA 
CAAAGATGTC CATTGGGTTT CTATGGTAAG GATTGTGTGC AGGCATGTCA ATGTGAGAAT 
GGTGCAGACT GCAATCACAT CTCGGGTCAG TGCACATGCC GTACAGGCTT CATGGGACGT 
CACTGTGAAA CGAAATGTCC TGCGGGTTCA TATGGATATG GCTGCCGGCA AGTTTGTGAT 
TGTCTCAATA ACTCCACCTG TGATCACATG ACTGGCACAT GTTACTGCAA CCCGGGCTGG 
AAAGGCACAC GATGTGATCA GGCTGGAGGG AATATTGCAG AAAGTCCCAA TAGTTTGACA 
AGCGCAGCTC TGCCTATGGA TTCATATCAA ATTGGCGCAA TCACAGGAAT CATCATCCTT 
GTACTTCTTG TGCTCATCCT CCTCCTTCTG TTCATCATTT ACCGCAAAAA ACAGAAAGGC 
AAAGAATCCT CGATGCCTTC GGTCACGTAT ACACCTACTA TGAGGGCCAA CACTGATTAC 
GCCATTGCAG AGTCACTCCC ACAAACAGAG GTCCTTCCCA ACAGTAACTA TTTCTCCAAC 
CCCAGCTACC ACACGCTGAC CCAATGCAGC AGCCCACCTC ACATCAATAA CATACCCTAT 
GGAAAGATGA ACAACAACCA GTTGTTTGTG AACCTGAAGA ACACTGAGCC GAGGAAGCGG 
CTTTCCCTGC TGGATCACAC AGGAACTCTA CCAGCTGACT GGAAGCAAGG CGGATCCTTT 
AGTGAACTCG GAGCTTATGG AGTTGACAGG CGCTACATGG GAAAGTCGCT TCGAGATCTT 
GTGAAGAGTT TGCCCTATCA TGCCAGTTCC TGCTCTCTGA ATAGTTCAGA GAACCCATAC 
GCCACCATCA AAGACCCTCC CCTGCTGTTG ACCAAGAGCA CTGAATGTGG TTATGTGGAA 
ATGAAGTCTC CTGCTCACAG AGATTCACCA TATGCTGAGA TACATTCCTC CAGCCCTGCC 
AACAAAAATG TCTATGAAGT TGAGCCAACC ATCAGTTCTG TCCAGGCACT TACAAACAAC 
AACTGCAACG GGCCATTCTG TCAAGATCCA TATGACTTAC CAAAGAATAG TCACATTCCA 
TGTCATTATG ACCTACTGCC TACAAGAGAC AGCAGCCCAT CACCCACAGA GGACAGCAAA 
TAA