slc12a4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a4
Entrez Gene ID	562826
Full Name	solute carrier family 12 (potassium/chloride transporter), member 4
Synonyms	im:7162646
General protein information	Preferred Names solute carrier family 12 (potassium/chloride transporter), member 4 Names solute carrier family 12 member 4 solute carrier family 12 (potassium/chloride transporters), member 4 solute carrier family 12, member 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_686199.6	XP_691291.2	solute carrier family 12 member 4
XM_686199.7	XP_691291.2	solute carrier family 12 member 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC12A4	NP_005063.1
Gallus gallus (chicken)	SLC12A4	XP_003641964.1
Danio rerio (zebrafish)	slc12a4	XP_691291.2
Drosophila melanogaster (fruit fly)	kcc	NP_726377.1
Canis lupus familiaris (dog)	SLC12A4	NP_001010952.1
Rattus norvegicus (Norway rat)	Slc12a4	NP_062102.1
Caenorhabditis elegans (roundworm)	kcc-2	NP_001255280.1
Pan troglodytes (chimpanzee)	SLC12A4	XP_003315222.2
	SLC12A4	XP_001096326.2
Bos taurus (cattle)	SLC12A4	NP_001167562.1
Mus musculus (house mouse)	Slc12a4	NP_001240733.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011498	XP_317803.3
Xenopus tropicalis (tropical clawed frog)	LOC100493471	XP_002942716.1

Gene Ontology

The following slc12a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13248	XM_686199.7 Latest version!	Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa13248	XM_686199.6	Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a4 ( XM_686199.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_691291.2 Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

Price & Availability↑

CloneID	ODa13248
Clone ID Related Accession (Same CDS sequence)	XM_686199.6 , XM_686199.7
Accession Version	XM_686199.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	solute carrier family 12 member 4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_686199.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TCACTGTGGT ACCAGTGCAG GATAACTCAC CGTCCAACTA TGATAGCCTG 
GAAGGAATAA ACTGGGTTGA TTACCGGGAC ACAGGACAGG GAGAATACCC TGGACATGGA 
GACACAGTTA GCTCAGATGG TCATGGTAAT CATAAAGAAG ACAGTCCCTT CCTCAGCAGT 
TCAGACTCAG CATCAAAGAG GAATGACTTC TACGACAGAA ACCTGGCCCT GTTTGAGGAA 
GAGCTGGACA TCCGGCCGAA GGTCTCGTCT CTTCTCAGCC GCCTGGTCAA TTACACAAAT 
GTTACTCAGG GTGCCAAAGA ACATGAAGAG GCAGAAAGTG CAGATGCATC CAGACGACAA 
GCACCCAAGT CTCCAAATAT GGGCACGCTA ATGGGAGTTT ACCTGCCGTG TCTGCAGAAC 
ATCTTTGGTG TGATTCTGTT TCTGCGTCTC ACCTGGATAG TAGGAACAGC AGGTGTCGTC 
CAGTCCTTCC TCATCGTTCT CATGTGCTGT TCCTGCACAA TGTTGACAGC AATCTCGATG 
AGTGCCATAG CCACCAATGG TGTAGTTCCA GCTGGTGGGG CTTATTTCAT GATATCTCGC 
TCTCTGGGCC CTGAGTTCGG TGGTGCTGTT GGTCTCTGTT TTTATTTAGG TACAACCTTC 
GCTGCTGCCA TGTACATATT GGGAGCAATT GAAATATTTC TGAAATATCT GATTCCTCAG 
GCTGCCATAT TCCATCCAGC AGATATTCAC GCAGGAAACG GTGCAATGCT GAATAACATG 
CGGGTTTATG GCACAATATG TTTAAGCCTC ATGGCTGTGG TTGTTTTTGT GGGGGTCAAA 
TATGTCAACA AATTAGCCTC GCTGTTCCTA GCCTGCGTCA TAATCTCCAT TGTTTCTATC 
TATGCTGGAG CGGTCAAGTC CATCTTCCAC CCTCCTGAGT TTCCGATCTG CATGTTAGGA 
AACCGGACAT TGGTTCGTGA TTTGTTTGAT GTTTGTGGGA AGACGGTAGT TGTCGGTAAT 
GAAACGGTCC CCAGCAAGTT GTGGAAGAAC TTCTGCACCT CTGATAACAT CAGCAGTGCT 
CACTGTGATG AGTACTTCTT CCACAACAAT GTTACAGAGA TCAAGGGCAT TCCTGGGCTG 
GCCAGTGGCA TCATTAAAGA AAACATGTGG GGGGATTACA TGGAGAAAGG ACAGATTCTA 
GAGAAGGCAA AACTGCCCTC TGTTGATGTC CATGGTTCCA TGGAGACATT TGGCTTCTAC 
GTGTCTGCTG ACATCGCTAC ATCCTTCACC CTTTTAGTGG GGATATTTTT TCCTTCTGCC 
ACAGGAATTA TGGCAGGATC AAACAGATCT GGAGATCTAA GAGACGCACA GAAGTCTATA 
CCAATTGGGA CGATTCTGGC CATAACAACG ACCTCTCTTG TCTATTTTAG CTCTGTGGTT 
TTGTTCGGAG CCTGTATTGA GGGTGTGGTG CTCAGAGATA AATTTGGAGA TGCAGTGAGT 
AAAAACCTAG TAGTTGGCAC ACTGTCCTGG CCCTCACCCT GGGTCATTGT GATTGGCTCC 
TTCTTCTCCA CCGTGGGTGC AGGTCTTCAG TCCCTGACCG GAGCTCCTCG TCTCCTGCAA 
GCCATCGCTA AAGATAACAT CATTCCTTTC CTCAGAGTCT TTGGACACGG TAAAGCTAAT 
GGAGAACCCA CATGGGCCCT GCTGCTGACA GGACTCATAG CAGAGCTTGG CATCCTCATT 
GCATCACTGG ATATGGTCGC TCCTATTCTC TCCATGTTTT TCCTGATGTG TTATCTATTT 
GTAAACTTGG CATGTGCAGT ACAAACACTA TTAAGGACAC CAAACTGGAG ACCGCGGTTC 
AGATATTACC ATTGGGCTCT GTCTTTTCTG GGCATGAGTA TGTGTTTAGC CCTTATGTTC 
ATCTCCTCTT GGTACTATGC CATTGTAGCG ATGGGCATTG CCGGAATGAT CTACAAGTAC 
ATTGAGTATC AGGGAGCAGA GAAGGAATGG GGTGATGGGA TCCGTGGTTT GTCTCTGAGC 
GCCGCCCGCT ACTCTCTGCT TCGCCTGGAG GCTGGACCAC CCCACACCAA AAACTGGAGG 
CCTCAGCTGC TGGTTCTGTT GAAGTTGGAT GAGGATCTGC ATGTAAAGTA TCCCAGATTG 
CTTACCTTTG CCTCACAGCT GAAGGCAGGG AAAGGCCTCA CCATTGTAGG ATCTGTGATT 
CAGGGAAACT TCCTGGAATG CTATGGGGAA ACACAAGCCT CAGAACAGGC GATCAAGAAC 
ATGATGGAGA TAGAGCGAGT GAAGGGCTTC TGTCAGGTGG TGGTCGCCTC TAAAGTCAGA 
GAAGGGATTG CGCACCTCAT CCAGTCCTGT GGTCTGGGAG GCATGAAGCA CAACACTGTT 
GTTATGGGCT GGCCATATGG CTGGAGACAG AGTGAAGACC CACGGGCTTG GAAAACCTTT 
ATTAATACAG TCCGCTGTAC CACAGCTGCA CACCTAGCTT TGATGGTGCC AAAGAATGTG 
TCATTCTATC CCAGCAATCA TGAGCGTTTT ACTGATGGAT ATATTGATGT CTGGTGGATT 
GTGCATGACG GTGGAATGCT CATGCTCCTG CCTTTCCTTC TCAAACAACA CAAGGTGTGG 
CGTAAATGTA AGATGCGAAT CTTCACTGTG GCTCAAATGG ATGATAACAG TATTCAGATG 
AAGAAGGATC TGGCAACCTT CCTATACCAG CTCAGATTAG AAGCAGAGGT GGAAGTGGTC 
GAGATGCACG ACAGCGACAT CTCAGCATAT ACGTATGAGC GGACACTGAT GATGGAGCAG 
AGGTCACAGA TGCTGAAACA AATGAGGCTC TCCAGTGCAG AAAGAGACAG AGAGGCCCAG 
CTAGTGAAAG ACAGACACTC ACTGATAAGG ATGGGCAGTT TATACTCGGA TGAAGAAGAG 
GAAACCATTG AGATATTACC AGAGAAAAAC CAGATGACCT GGACTAGTGA AAAGATAGAA 
GCAGAGAGAC GTAACCGAAA CAACGCACCA GAAAACTTCA GAGAGCTAAT AAGCATTAAA 
CCTGACCAGT CTAATGTCCG GCGGATGCAC ACAGCTGTGA AGCTGAATGA GGTTATTGTC 
AACAAATCCC ATGATGCCAG ACTTGTGCTG CTAAATATGC CAGGACCTCC ACGCAATCAA 
GAGGGAGATG AAAACTATAT GGAGTTTCTC GAGGTATTAA CTGAGGGGCT AGAACGAGTG 
CTGCTTGTAA GAGGTGGAGG TCGTGAGGTC ATCACCATCT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_691291.2
CDS	276..3563
Translation	MPHFTVVPVQDNSPSNYDSLEGINWVDYRDTGQGEYPGHGDTVSSDGHGNHKEDSPFLSSSDSASKRNDFYDRNLALFEEELDIRPKVSSLLSRLVNYTNVTQGAKEHEEAESADASRRQAPKSPNMGTLMGVYLPCLQNIFGVILFLRLTWIVGTAGVVQSFLIVLMCCSCTMLTAISMSAIATNGVVPAGGAYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLKYLIPQAAIFHPADIHAGNGAMLNNMRVYGTICLSLMAVVVFVGVKYVNKLASLFLACVIISIVSIYAGAVKSIFHPPEFPICMLGNRTLVRDLFDVCGKTVVVGNETVPSKLWKNFCTSDNISSAHCDEYFFHNNVTEIKGIPGLASGIIKENMWGDYMEKGQILEKAKLPSVDVHGSMETFGFYVSADIATSFTLLVGIFFPSATGIMAGSNRSGDLRDAQKSIPIGTILAITTTSLVYFSSVVLFGACIEGVVLRDKFGDAVSKNLVVGTLSWPSPWVIVIGSFFSTVGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTGLIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFRYYHWALSFLGMSMCLALMFISSWYYAIVAMGIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYSLLRLEAGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGNFLECYGETQASEQAIKNMMEIERVKGFCQVVVASKVREGIAHLIQSCGLGGMKHNTVVMGWPYGWRQSEDPRAWKTFINTVRCTTAAHLALMVPKNVSFYPSNHERFTDGYIDVWWIVHDGGMLMLLPFLLKQHKVWRKCKMRIFTVAQMDDNSIQMKKDLATFLYQLRLEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLKQMRLSSAERDREAQLVKDRHSLIRMGSLYSDEEEETIEILPEKNQMTWTSEKIEAERRNRNNAPENFRELISIKPDQSNVRRMHTAVKLNEVIVNKSHDARLVLLNMPGPPRNQEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_686199.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_686199.6

ORF Insert Sequence:

ATGCCTCATT TCACTGTGGT ACCAGTGCAG GATAACTCAC CGTCCAACTA TGATAGCCTG 
GAAGGAATAA ACTGGGTTGA TTACCGGGAC ACAGGACAGG GAGAATACCC TGGACATGGA 
GACACAGTTA GCTCAGATGG TCATGGTAAT CATAAAGAAG ACAGTCCCTT CCTCAGCAGT 
TCAGACTCAG CATCAAAGAG GAATGACTTC TACGACAGAA ACCTGGCCCT GTTTGAGGAA 
GAGCTGGACA TCCGGCCGAA GGTCTCGTCT CTTCTCAGCC GCCTGGTCAA TTACACAAAT 
GTTACTCAGG GTGCCAAAGA ACATGAAGAG GCAGAAAGTG CAGATGCATC CAGACGACAA 
GCACCCAAGT CTCCAAATAT GGGCACGCTA ATGGGAGTTT ACCTGCCGTG TCTGCAGAAC 
ATCTTTGGTG TGATTCTGTT TCTGCGTCTC ACCTGGATAG TAGGAACAGC AGGTGTCGTC 
CAGTCCTTCC TCATCGTTCT CATGTGCTGT TCCTGCACAA TGTTGACAGC AATCTCGATG 
AGTGCCATAG CCACCAATGG TGTAGTTCCA GCTGGTGGGG CTTATTTCAT GATATCTCGC 
TCTCTGGGCC CTGAGTTCGG TGGTGCTGTT GGTCTCTGTT TTTATTTAGG TACAACCTTC 
GCTGCTGCCA TGTACATATT GGGAGCAATT GAAATATTTC TGAAATATCT GATTCCTCAG 
GCTGCCATAT TCCATCCAGC AGATATTCAC GCAGGAAACG GTGCAATGCT GAATAACATG 
CGGGTTTATG GCACAATATG TTTAAGCCTC ATGGCTGTGG TTGTTTTTGT GGGGGTCAAA 
TATGTCAACA AATTAGCCTC GCTGTTCCTA GCCTGCGTCA TAATCTCCAT TGTTTCTATC 
TATGCTGGAG CGGTCAAGTC CATCTTCCAC CCTCCTGAGT TTCCGATCTG CATGTTAGGA 
AACCGGACAT TGGTTCGTGA TTTGTTTGAT GTTTGTGGGA AGACGGTAGT TGTCGGTAAT 
GAAACGGTCC CCAGCAAGTT GTGGAAGAAC TTCTGCACCT CTGATAACAT CAGCAGTGCT 
CACTGTGATG AGTACTTCTT CCACAACAAT GTTACAGAGA TCAAGGGCAT TCCTGGGCTG 
GCCAGTGGCA TCATTAAAGA AAACATGTGG GGGGATTACA TGGAGAAAGG ACAGATTCTA 
GAGAAGGCAA AACTGCCCTC TGTTGATGTC CATGGTTCCA TGGAGACATT TGGCTTCTAC 
GTGTCTGCTG ACATCGCTAC ATCCTTCACC CTTTTAGTGG GGATATTTTT TCCTTCTGCC 
ACAGGAATTA TGGCAGGATC AAACAGATCT GGAGATCTAA GAGACGCACA GAAGTCTATA 
CCAATTGGGA CGATTCTGGC CATAACAACG ACCTCTCTTG TCTATTTTAG CTCTGTGGTT 
TTGTTCGGAG CCTGTATTGA GGGTGTGGTG CTCAGAGATA AATTTGGAGA TGCAGTGAGT 
AAAAACCTAG TAGTTGGCAC ACTGTCCTGG CCCTCACCCT GGGTCATTGT GATTGGCTCC 
TTCTTCTCCA CCGTGGGTGC AGGTCTTCAG TCCCTGACCG GAGCTCCTCG TCTCCTGCAA 
GCCATCGCTA AAGATAACAT CATTCCTTTC CTCAGAGTCT TTGGACACGG TAAAGCTAAT 
GGAGAACCCA CATGGGCCCT GCTGCTGACA GGACTCATAG CAGAGCTTGG CATCCTCATT 
GCATCACTGG ATATGGTCGC TCCTATTCTC TCCATGTTTT TCCTGATGTG TTATCTATTT 
GTAAACTTGG CATGTGCAGT ACAAACACTA TTAAGGACAC CAAACTGGAG ACCGCGGTTC 
AGATATTACC ATTGGGCTCT GTCTTTTCTG GGCATGAGTA TGTGTTTAGC CCTTATGTTC 
ATCTCCTCTT GGTACTATGC CATTGTAGCG ATGGGCATTG CCGGAATGAT CTACAAGTAC 
ATTGAGTATC AGGGAGCAGA GAAGGAATGG GGTGATGGGA TCCGTGGTTT GTCTCTGAGC 
GCCGCCCGCT ACTCTCTGCT TCGCCTGGAG GCTGGACCAC CCCACACCAA AAACTGGAGG 
CCTCAGCTGC TGGTTCTGTT GAAGTTGGAT GAGGATCTGC ATGTAAAGTA TCCCAGATTG 
CTTACCTTTG CCTCACAGCT GAAGGCAGGG AAAGGCCTCA CCATTGTAGG ATCTGTGATT 
CAGGGAAACT TCCTGGAATG CTATGGGGAA ACACAAGCCT CAGAACAGGC GATCAAGAAC 
ATGATGGAGA TAGAGCGAGT GAAGGGCTTC TGTCAGGTGG TGGTCGCCTC TAAAGTCAGA 
GAAGGGATTG CGCACCTCAT CCAGTCCTGT GGTCTGGGAG GCATGAAGCA CAACACTGTT 
GTTATGGGCT GGCCATATGG CTGGAGACAG AGTGAAGACC CACGGGCTTG GAAAACCTTT 
ATTAATACAG TCCGCTGTAC CACAGCTGCA CACCTAGCTT TGATGGTGCC AAAGAATGTG 
TCATTCTATC CCAGCAATCA TGAGCGTTTT ACTGATGGAT ATATTGATGT CTGGTGGATT 
GTGCATGACG GTGGAATGCT CATGCTCCTG CCTTTCCTTC TCAAACAACA CAAGGTGTGG 
CGTAAATGTA AGATGCGAAT CTTCACTGTG GCTCAAATGG ATGATAACAG TATTCAGATG 
AAGAAGGATC TGGCAACCTT CCTATACCAG CTCAGATTAG AAGCAGAGGT GGAAGTGGTC 
GAGATGCACG ACAGCGACAT CTCAGCATAT ACGTATGAGC GGACACTGAT GATGGAGCAG 
AGGTCACAGA TGCTGAAACA AATGAGGCTC TCCAGTGCAG AAAGAGACAG AGAGGCCCAG 
CTAGTGAAAG ACAGACACTC ACTGATAAGG ATGGGCAGTT TATACTCGGA TGAAGAAGAG 
GAAACCATTG AGATATTACC AGAGAAAAAC CAGATGACCT GGACTAGTGA AAAGATAGAA 
GCAGAGAGAC GTAACCGAAA CAACGCACCA GAAAACTTCA GAGAGCTAAT AAGCATTAAA 
CCTGACCAGT CTAATGTCCG GCGGATGCAC ACAGCTGTGA AGCTGAATGA GGTTATTGTC 
AACAAATCCC ATGATGCCAG ACTTGTGCTG CTAAATATGC CAGGACCTCC ACGCAATCAA 
GAGGGAGATG AAAACTATAT GGAGTTTCTC GAGGTATTAA CTGAGGGGCT AGAACGAGTG 
CTGCTTGTAA GAGGTGGAGG TCGTGAGGTC ATCACCATCT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a4 ( XM_686199.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_691291.2 Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

Price & Availability↑

CloneID	ODa13248
Clone ID Related Accession (Same CDS sequence)	XM_686199.6 , XM_686199.7
Accession Version	XM_686199.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_686199.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TCACTGTGGT ACCAGTGCAG GATAACTCAC CGTCCAACTA TGATAGCCTG 
GAAGGAATAA ACTGGGTTGA TTACCGGGAC ACAGGACAGG GAGAATACCC TGGACATGGA 
GACACAGTTA GCTCAGATGG TCATGGTAAT CATAAAGAAG ACAGTCCCTT CCTCAGCAGT 
TCAGACTCAG CATCAAAGAG GAATGACTTC TACGACAGAA ACCTGGCCCT GTTTGAGGAA 
GAGCTGGACA TCCGGCCGAA GGTCTCGTCT CTTCTCAGCC GCCTGGTCAA TTACACAAAT 
GTTACTCAGG GTGCCAAAGA ACATGAAGAG GCAGAAAGTG CAGATGCATC CAGACGACAA 
GCACCCAAGT CTCCAAATAT GGGCACGCTA ATGGGAGTTT ACCTGCCGTG TCTGCAGAAC 
ATCTTTGGTG TGATTCTGTT TCTGCGTCTC ACCTGGATAG TAGGAACAGC AGGTGTCGTC 
CAGTCCTTCC TCATCGTTCT CATGTGCTGT TCCTGCACAA TGTTGACAGC AATCTCGATG 
AGTGCCATAG CCACCAATGG TGTAGTTCCA GCTGGTGGGG CTTATTTCAT GATATCTCGC 
TCTCTGGGCC CTGAGTTCGG TGGTGCTGTT GGTCTCTGTT TTTATTTAGG TACAACCTTC 
GCTGCTGCCA TGTACATATT GGGAGCAATT GAAATATTTC TGAAATATCT GATTCCTCAG 
GCTGCCATAT TCCATCCAGC AGATATTCAC GCAGGAAACG GTGCAATGCT GAATAACATG 
CGGGTTTATG GCACAATATG TTTAAGCCTC ATGGCTGTGG TTGTTTTTGT GGGGGTCAAA 
TATGTCAACA AATTAGCCTC GCTGTTCCTA GCCTGCGTCA TAATCTCCAT TGTTTCTATC 
TATGCTGGAG CGGTCAAGTC CATCTTCCAC CCTCCTGAGT TTCCGATCTG CATGTTAGGA 
AACCGGACAT TGGTTCGTGA TTTGTTTGAT GTTTGTGGGA AGACGGTAGT TGTCGGTAAT 
GAAACGGTCC CCAGCAAGTT GTGGAAGAAC TTCTGCACCT CTGATAACAT CAGCAGTGCT 
CACTGTGATG AGTACTTCTT CCACAACAAT GTTACAGAGA TCAAGGGCAT TCCTGGGCTG 
GCCAGTGGCA TCATTAAAGA AAACATGTGG GGGGATTACA TGGAGAAAGG ACAGATTCTA 
GAGAAGGCAA AACTGCCCTC TGTTGATGTC CATGGTTCCA TGGAGACATT TGGCTTCTAC 
GTGTCTGCTG ACATCGCTAC ATCCTTCACC CTTTTAGTGG GGATATTTTT TCCTTCTGCC 
ACAGGAATTA TGGCAGGATC AAACAGATCT GGAGATCTAA GAGACGCACA GAAGTCTATA 
CCAATTGGGA CGATTCTGGC CATAACAACG ACCTCTCTTG TCTATTTTAG CTCTGTGGTT 
TTGTTCGGAG CCTGTATTGA GGGTGTGGTG CTCAGAGATA AATTTGGAGA TGCAGTGAGT 
AAAAACCTAG TAGTTGGCAC ACTGTCCTGG CCCTCACCCT GGGTCATTGT GATTGGCTCC 
TTCTTCTCCA CCGTGGGTGC AGGTCTTCAG TCCCTGACCG GAGCTCCTCG TCTCCTGCAA 
GCCATCGCTA AAGATAACAT CATTCCTTTC CTCAGAGTCT TTGGACACGG TAAAGCTAAT 
GGAGAACCCA CATGGGCCCT GCTGCTGACA GGACTCATAG CAGAGCTTGG CATCCTCATT 
GCATCACTGG ATATGGTCGC TCCTATTCTC TCCATGTTTT TCCTGATGTG TTATCTATTT 
GTAAACTTGG CATGTGCAGT ACAAACACTA TTAAGGACAC CAAACTGGAG ACCGCGGTTC 
AGATATTACC ATTGGGCTCT GTCTTTTCTG GGCATGAGTA TGTGTTTAGC CCTTATGTTC 
ATCTCCTCTT GGTACTATGC CATTGTAGCG ATGGGCATTG CCGGAATGAT CTACAAGTAC 
ATTGAGTATC AGGGAGCAGA GAAGGAATGG GGTGATGGGA TCCGTGGTTT GTCTCTGAGC 
GCCGCCCGCT ACTCTCTGCT TCGCCTGGAG GCTGGACCAC CCCACACCAA AAACTGGAGG 
CCTCAGCTGC TGGTTCTGTT GAAGTTGGAT GAGGATCTGC ATGTAAAGTA TCCCAGATTG 
CTTACCTTTG CCTCACAGCT GAAGGCAGGG AAAGGCCTCA CCATTGTAGG ATCTGTGATT 
CAGGGAAACT TCCTGGAATG CTATGGGGAA ACACAAGCCT CAGAACAGGC GATCAAGAAC 
ATGATGGAGA TAGAGCGAGT GAAGGGCTTC TGTCAGGTGG TGGTCGCCTC TAAAGTCAGA 
GAAGGGATTG CGCACCTCAT CCAGTCCTGT GGTCTGGGAG GCATGAAGCA CAACACTGTT 
GTTATGGGCT GGCCATATGG CTGGAGACAG AGTGAAGACC CACGGGCTTG GAAAACCTTT 
ATTAATACAG TCCGCTGTAC CACAGCTGCA CACCTAGCTT TGATGGTGCC AAAGAATGTG 
TCATTCTATC CCAGCAATCA TGAGCGTTTT ACTGATGGAT ATATTGATGT CTGGTGGATT 
GTGCATGACG GTGGAATGCT CATGCTCCTG CCTTTCCTTC TCAAACAACA CAAGGTGTGG 
CGTAAATGTA AGATGCGAAT CTTCACTGTG GCTCAAATGG ATGATAACAG TATTCAGATG 
AAGAAGGATC TGGCAACCTT CCTATACCAG CTCAGATTAG AAGCAGAGGT GGAAGTGGTC 
GAGATGCACG ACAGCGACAT CTCAGCATAT ACGTATGAGC GGACACTGAT GATGGAGCAG 
AGGTCACAGA TGCTGAAACA AATGAGGCTC TCCAGTGCAG AAAGAGACAG AGAGGCCCAG 
CTAGTGAAAG ACAGACACTC ACTGATAAGG ATGGGCAGTT TATACTCGGA TGAAGAAGAG 
GAAACCATTG AGATATTACC AGAGAAAAAC CAGATGACCT GGACTAGTGA AAAGATAGAA 
GCAGAGAGAC GTAACCGAAA CAACGCACCA GAAAACTTCA GAGAGCTAAT AAGCATTAAA 
CCTGACCAGT CTAATGTCCG GCGGATGCAC ACAGCTGTGA AGCTGAATGA GGTTATTGTC 
AACAAATCCC ATGATGCCAG ACTTGTGCTG CTAAATATGC CAGGACCTCC ACGCAATCAA 
GAGGGAGATG AAAACTATAT GGAGTTTCTC GAGGTATTAA CTGAGGGGCT AGAACGAGTG 
CTGCTTGTAA GAGGTGGAGG TCGTGAGGTC ATCACCATCT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_691291.2
CDS	262..3549
Translation	MPHFTVVPVQDNSPSNYDSLEGINWVDYRDTGQGEYPGHGDTVSSDGHGNHKEDSPFLSSSDSASKRNDFYDRNLALFEEELDIRPKVSSLLSRLVNYTNVTQGAKEHEEAESADASRRQAPKSPNMGTLMGVYLPCLQNIFGVILFLRLTWIVGTAGVVQSFLIVLMCCSCTMLTAISMSAIATNGVVPAGGAYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLKYLIPQAAIFHPADIHAGNGAMLNNMRVYGTICLSLMAVVVFVGVKYVNKLASLFLACVIISIVSIYAGAVKSIFHPPEFPICMLGNRTLVRDLFDVCGKTVVVGNETVPSKLWKNFCTSDNISSAHCDEYFFHNNVTEIKGIPGLASGIIKENMWGDYMEKGQILEKAKLPSVDVHGSMETFGFYVSADIATSFTLLVGIFFPSATGIMAGSNRSGDLRDAQKSIPIGTILAITTTSLVYFSSVVLFGACIEGVVLRDKFGDAVSKNLVVGTLSWPSPWVIVIGSFFSTVGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTGLIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFRYYHWALSFLGMSMCLALMFISSWYYAIVAMGIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYSLLRLEAGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGNFLECYGETQASEQAIKNMMEIERVKGFCQVVVASKVREGIAHLIQSCGLGGMKHNTVVMGWPYGWRQSEDPRAWKTFINTVRCTTAAHLALMVPKNVSFYPSNHERFTDGYIDVWWIVHDGGMLMLLPFLLKQHKVWRKCKMRIFTVAQMDDNSIQMKKDLATFLYQLRLEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLKQMRLSSAERDREAQLVKDRHSLIRMGSLYSDEEEETIEILPEKNQMTWTSEKIEAERRNRNNAPENFRELISIKPDQSNVRRMHTAVKLNEVIVNKSHDARLVLLNMPGPPRNQEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_686199.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_686199.7

ORF Insert Sequence:

ATGCCTCATT TCACTGTGGT ACCAGTGCAG GATAACTCAC CGTCCAACTA TGATAGCCTG 
GAAGGAATAA ACTGGGTTGA TTACCGGGAC ACAGGACAGG GAGAATACCC TGGACATGGA 
GACACAGTTA GCTCAGATGG TCATGGTAAT CATAAAGAAG ACAGTCCCTT CCTCAGCAGT 
TCAGACTCAG CATCAAAGAG GAATGACTTC TACGACAGAA ACCTGGCCCT GTTTGAGGAA 
GAGCTGGACA TCCGGCCGAA GGTCTCGTCT CTTCTCAGCC GCCTGGTCAA TTACACAAAT 
GTTACTCAGG GTGCCAAAGA ACATGAAGAG GCAGAAAGTG CAGATGCATC CAGACGACAA 
GCACCCAAGT CTCCAAATAT GGGCACGCTA ATGGGAGTTT ACCTGCCGTG TCTGCAGAAC 
ATCTTTGGTG TGATTCTGTT TCTGCGTCTC ACCTGGATAG TAGGAACAGC AGGTGTCGTC 
CAGTCCTTCC TCATCGTTCT CATGTGCTGT TCCTGCACAA TGTTGACAGC AATCTCGATG 
AGTGCCATAG CCACCAATGG TGTAGTTCCA GCTGGTGGGG CTTATTTCAT GATATCTCGC 
TCTCTGGGCC CTGAGTTCGG TGGTGCTGTT GGTCTCTGTT TTTATTTAGG TACAACCTTC 
GCTGCTGCCA TGTACATATT GGGAGCAATT GAAATATTTC TGAAATATCT GATTCCTCAG 
GCTGCCATAT TCCATCCAGC AGATATTCAC GCAGGAAACG GTGCAATGCT GAATAACATG 
CGGGTTTATG GCACAATATG TTTAAGCCTC ATGGCTGTGG TTGTTTTTGT GGGGGTCAAA 
TATGTCAACA AATTAGCCTC GCTGTTCCTA GCCTGCGTCA TAATCTCCAT TGTTTCTATC 
TATGCTGGAG CGGTCAAGTC CATCTTCCAC CCTCCTGAGT TTCCGATCTG CATGTTAGGA 
AACCGGACAT TGGTTCGTGA TTTGTTTGAT GTTTGTGGGA AGACGGTAGT TGTCGGTAAT 
GAAACGGTCC CCAGCAAGTT GTGGAAGAAC TTCTGCACCT CTGATAACAT CAGCAGTGCT 
CACTGTGATG AGTACTTCTT CCACAACAAT GTTACAGAGA TCAAGGGCAT TCCTGGGCTG 
GCCAGTGGCA TCATTAAAGA AAACATGTGG GGGGATTACA TGGAGAAAGG ACAGATTCTA 
GAGAAGGCAA AACTGCCCTC TGTTGATGTC CATGGTTCCA TGGAGACATT TGGCTTCTAC 
GTGTCTGCTG ACATCGCTAC ATCCTTCACC CTTTTAGTGG GGATATTTTT TCCTTCTGCC 
ACAGGAATTA TGGCAGGATC AAACAGATCT GGAGATCTAA GAGACGCACA GAAGTCTATA 
CCAATTGGGA CGATTCTGGC CATAACAACG ACCTCTCTTG TCTATTTTAG CTCTGTGGTT 
TTGTTCGGAG CCTGTATTGA GGGTGTGGTG CTCAGAGATA AATTTGGAGA TGCAGTGAGT 
AAAAACCTAG TAGTTGGCAC ACTGTCCTGG CCCTCACCCT GGGTCATTGT GATTGGCTCC 
TTCTTCTCCA CCGTGGGTGC AGGTCTTCAG TCCCTGACCG GAGCTCCTCG TCTCCTGCAA 
GCCATCGCTA AAGATAACAT CATTCCTTTC CTCAGAGTCT TTGGACACGG TAAAGCTAAT 
GGAGAACCCA CATGGGCCCT GCTGCTGACA GGACTCATAG CAGAGCTTGG CATCCTCATT 
GCATCACTGG ATATGGTCGC TCCTATTCTC TCCATGTTTT TCCTGATGTG TTATCTATTT 
GTAAACTTGG CATGTGCAGT ACAAACACTA TTAAGGACAC CAAACTGGAG ACCGCGGTTC 
AGATATTACC ATTGGGCTCT GTCTTTTCTG GGCATGAGTA TGTGTTTAGC CCTTATGTTC 
ATCTCCTCTT GGTACTATGC CATTGTAGCG ATGGGCATTG CCGGAATGAT CTACAAGTAC 
ATTGAGTATC AGGGAGCAGA GAAGGAATGG GGTGATGGGA TCCGTGGTTT GTCTCTGAGC 
GCCGCCCGCT ACTCTCTGCT TCGCCTGGAG GCTGGACCAC CCCACACCAA AAACTGGAGG 
CCTCAGCTGC TGGTTCTGTT GAAGTTGGAT GAGGATCTGC ATGTAAAGTA TCCCAGATTG 
CTTACCTTTG CCTCACAGCT GAAGGCAGGG AAAGGCCTCA CCATTGTAGG ATCTGTGATT 
CAGGGAAACT TCCTGGAATG CTATGGGGAA ACACAAGCCT CAGAACAGGC GATCAAGAAC 
ATGATGGAGA TAGAGCGAGT GAAGGGCTTC TGTCAGGTGG TGGTCGCCTC TAAAGTCAGA 
GAAGGGATTG CGCACCTCAT CCAGTCCTGT GGTCTGGGAG GCATGAAGCA CAACACTGTT 
GTTATGGGCT GGCCATATGG CTGGAGACAG AGTGAAGACC CACGGGCTTG GAAAACCTTT 
ATTAATACAG TCCGCTGTAC CACAGCTGCA CACCTAGCTT TGATGGTGCC AAAGAATGTG 
TCATTCTATC CCAGCAATCA TGAGCGTTTT ACTGATGGAT ATATTGATGT CTGGTGGATT 
GTGCATGACG GTGGAATGCT CATGCTCCTG CCTTTCCTTC TCAAACAACA CAAGGTGTGG 
CGTAAATGTA AGATGCGAAT CTTCACTGTG GCTCAAATGG ATGATAACAG TATTCAGATG 
AAGAAGGATC TGGCAACCTT CCTATACCAG CTCAGATTAG AAGCAGAGGT GGAAGTGGTC 
GAGATGCACG ACAGCGACAT CTCAGCATAT ACGTATGAGC GGACACTGAT GATGGAGCAG 
AGGTCACAGA TGCTGAAACA AATGAGGCTC TCCAGTGCAG AAAGAGACAG AGAGGCCCAG 
CTAGTGAAAG ACAGACACTC ACTGATAAGG ATGGGCAGTT TATACTCGGA TGAAGAAGAG 
GAAACCATTG AGATATTACC AGAGAAAAAC CAGATGACCT GGACTAGTGA AAAGATAGAA 
GCAGAGAGAC GTAACCGAAA CAACGCACCA GAAAACTTCA GAGAGCTAAT AAGCATTAAA 
CCTGACCAGT CTAATGTCCG GCGGATGCAC ACAGCTGTGA AGCTGAATGA GGTTATTGTC 
AACAAATCCC ATGATGCCAG ACTTGTGCTG CTAAATATGC CAGGACCTCC ACGCAATCAA 
GAGGGAGATG AAAACTATAT GGAGTTTCTC GAGGTATTAA CTGAGGGGCT AGAACGAGTG 
CTGCTTGTAA GAGGTGGAGG TCGTGAGGTC ATCACCATCT ACTCTTAA