smarcal1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smarcal1
Entrez Gene ID	560412
Full Name	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
Synonyms	im:7145331,si:ch211-239d11.1
General protein information	Preferred Names SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 Names SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 hepA-related protein sucrose nonfermenting protein 2-like 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009304989.2	XP_009303264.1	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 isoform X2
XM_021478691.1	XP_021334366.1	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 isoform X1
XM_009304988.2	XP_009303263.1	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 isoform X1
NM_001127466.1	NP_001120938.1	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

Pathways from BioSystems

Homologies

	SMARCAL1	XP_001086594.1
Xenopus tropicalis (tropical clawed frog)	smarcal1	NP_001072923.1
Pan troglodytes (chimpanzee)	SMARCAL1	XP_001153124.1
Bos taurus (cattle)	SMARCAL1	NP_788839.1
Rattus norvegicus (Norway rat)	Smarcal1	NP_001101692.1
Gallus gallus (chicken)	SMARCAL1	XP_422070.3
Mus musculus (house mouse)	Smarcal1	NP_061287.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008426	XP_317019.4
Homo sapiens (human)	SMARCAL1	NP_001120679.1
Canis lupus familiaris (dog)	SMARCAL1	XP_005640678.1
Caenorhabditis elegans (roundworm)	C16A3.1	NP_741192.1
Danio rerio (zebrafish)	smarcal1	NP_001120938.1
Drosophila melanogaster (fruit fly)	Marcal1	NP_608883.1
Arabidopsis thaliana (thale cress)	CHR18	NP_175265.3
Oryza sativa (rice)	Os07g0636200	NP_001060393.1

Gene Ontology
Bibliography

Related articles in PubMed

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Deficiency of smarcal1 causes cell cycle arrest and developmental abnormalities in zebrafish.
Huang C, Gu S, Yu P, Yu F, Feng C, Gao N, Du J
Developmental biology339(1)89-100(2010 Mar)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

found that SMARCAL1 was transcriptionally inhibited by E2F6, an important cell cycle regulator. Over-expression of E2F6 in zebrafish embryos reduced the expression of smarcal1 mRNA and induced developmental defects similar to those in smarcal1 morphants
Title: Deficiency of smarcal1 causes cell cycle arrest and developmental abnormalities in zebrafish.

The following smarcal1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smarcal1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38766	NM_001127466.1 Latest version!	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa43286	XM_009304988.2 Latest version!	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa43287	XM_009304989.2 Latest version!	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa43287	XM_021478691.1 Latest version!	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smarcal1 ( NM_001127466.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001120938.1 Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), mRNA.

Price & Availability↑

CloneID	ODa38766
Clone ID Related Accession (Same CDS sequence)	NM_001127466.1
Accession Version	NM_001127466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU655703.1. On Jul 31, 2008 this sequence version replaced XM_001919669.1. ##Evidence-Data-START## Transcript exon combination :: EU655703.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGTGA GTCTGACTCC AGAACAGCAG CGGAGGATTG AAGAGAACAG AAAGAAAGCA 
TTAGCCAGAA GAGCTGAAAG ACAGGCTCAG ATCGCAGATC CTGGACCTGC TCAAAACAAA 
CACACACAGT CCGGGACAAC AGGAGGCCCA GCCAAAAACA ACCAGCATTT TGCGTCTGAT 
CGGGGACAGT CAGGGGCACC TACAAGACAG ACTCAGCTCA TAGACATTAA TGCAGCGTGT 
ACAAAAACAG CACCTCCGAC TTCCATTACT GCTGCCTCTA CAGCTTCTAG TTTTTATGGA 
CAGGCAGGAA AACCAAGCAC TGGAGAAAAG AGACCACCTA AACCCCCTGA GCCCGCGGCA 
AATATCCCTG CAAAGAAACC AGCGGTGTAC TTGAGGGGAA GATGTGTTTC ACACTCAGAA 
AACCGCTTCC GAGTGGAGGT GGGCTACCAT GCAGACCTCA TCCTAGTGTT CAAAAGCATT 
CCCTCCAAAA ACTACGATCC TGCTACGAAG ATGTGGAACT TCAGCCTTGA GGACTACCAG 
ATGCTCATGG AGCAGGTGGC ACATTTACCC TCCATCTCTC TCAAACCTCT GGAAGGGATG 
GAAGGGCTGA ATATTTCTGC ATCCACATGT CGGCCCAAAG ACGCTGCAGC AATGGCGGCT 
TTGATGCGCC TCTGTCAGGG TTGGCAGAAA CCGGGTGCTA CAATTAAAGG AAAGTGCGTT 
TTAGTGTCTC GCTCTCGACT GGAGGTGGAT ATTGGATACC AGGCTGATGT TATTGGGATT 
TTTAAGCAAA TGCCATCCAA AAGTTATGAC ATGAAGACCA GAAAGTGGAC CTTTCTCCTT 
GAGGACTATG GGAAACTCAT GGCGGATCTG AATGAACTGC CTACAGTGGA GACTGAGCCT 
TTACCTCACG CCGTCCTCCA GTCCTTCTCC AGTCAGTTTG AAAAAACCCA ATCCCAAGCT 
CCAGTCCCTC CTGAAGCAGA TCTCTCACAC ATCGATCCAC AACTCACACG CAGCCTCATG 
CCTTTCCAGA GAGACGGAGT CAATTTTGCA GTGTCCAGAG AGGGTCGTTT GCTCCTGGCT 
GATGATATGG GCCTCGGGAA GACGGTTCAG GCCATCTGCA TTGCTGCCTA TTACAGGAGT 
GAGTGGCCTC TGCTGGTGGT CGCCCCGTCC TCAGTGCGCT TCACATGGGC TGAGGCTTTC 
CGCCGATGGC TGCCTTCAGT GAAGCCAGAC AGCATTAATG TGGTGGTTAA AGGAAAAGAC 
AGCCTGCGCT CCGGCCTCAT CAACATCATC AGCTATGACC TGCTCAACAA GATGGACAAA 
CAGCCGCCGT CCAGCCCATT CAACGTCATC ATCATGGATG AGTCTCACTT CCTGAAGAAC 
ATGAAAACGG CTCGCTGTCG GGCGGCTCTT CCTCTGCTCA AGACAGCTAA GAGAGTGATC 
CTGCTGTCAG GAACGCCAGC CATGTCCAGA CCCGCTGAAC TCTACACTCA GATTCAGGCT 
GTCCGGCCCG CACTGTTTCC ACGCTTTCAT GACTTCGGCA CACGATACTG CGATGCCAAA 
CAGCTGCCGT GGGGTTGGGA TTACTCCAGC TCCTCTAATC TGACTGAACT CAAGCTGCTG 
CTAGAGGAGT CTCTGATGCT CCGACGCCTC AAATCAGAGG TGCTTTCACA GCTGCCTGCC 
AAACAGAGGA AAGTGGTCAC TGTAACCACA GACGGCATCA ACTCCAGGAC CAAAGCAGCC 
CTCAATGCCG CGGCCAGAGA GCTCGCCAAA GGATACCACA ACAAATCCCA GGAGAAAGAA 
GCACTCCTGG TTTTCTTCAA CCACACAGCA GAGGCCAAGA TCAGAGCTAT CATGGAGTAT 
ATCTCAGACA TGTTGGAGTG CGGACGAGAG AAGTTTCTGG TGTTTGCTCA TCATAAACTG 
GTGCTGGACA GTATAACCAA AGAACTTGGG GAGAAGAGCA TCAGCTTCAT CCGTATCGAC 
GGCTCGACAC CGTCAGCGGA GCGTCAGCTG CTGTGTGAGC GTTTCCAGGC GTCCCAGCAG 
AGCTGTGTGG CCGTTCTGTC CATCACCGCC GCTAACATGG GGCTCACACT GCACTCTGCA 
GCACTCGTGG TGTTCGCGGA GCTCTTTTGG AACCCTGGAG TCTTGATTCA GGCTGAGGAT 
CGGGTTCACA GAATCGGTCA GACCAGTAAT GTGGACATTC ATTACCTGGT GGCCAAAGGC 
ACAGCGGACG ATTACCTGTG GCCGATGATA CAAGCCAAAA TGAATGTTCT GGAACAGGTC 
GGCTTGTCCG AATCCAACAT TTCAGAAAAT GCAGAATCTG CCAGCTTCCA CAGCAGGGAT 
CGTCAGCAGC TCACCATCAC TGAGATGTTC CAGAGGTCTT TCGATGAGGA CGAGATGCTC 
GCTTTGATGG ATCAGGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120938.1
CDS	550..2973
Translation	MSVSLTPEQQRRIEENRKKALARRAERQAQIADPGPAQNKHTQSGTTGGPAKNNQHFASDRGQSGAPTRQTQLIDINAACTKTAPPTSITAASTASSFYGQAGKPSTGEKRPPKPPEPAANIPAKKPAVYLRGRCVSHSENRFRVEVGYHADLILVFKSIPSKNYDPATKMWNFSLEDYQMLMEQVAHLPSISLKPLEGMEGLNISASTCRPKDAAAMAALMRLCQGWQKPGATIKGKCVLVSRSRLEVDIGYQADVIGIFKQMPSKSYDMKTRKWTFLLEDYGKLMADLNELPTVETEPLPHAVLQSFSSQFEKTQSQAPVPPEADLSHIDPQLTRSLMPFQRDGVNFAVSREGRLLLADDMGLGKTVQAICIAAYYRSEWPLLVVAPSSVRFTWAEAFRRWLPSVKPDSINVVVKGKDSLRSGLINIISYDLLNKMDKQPPSSPFNVIIMDESHFLKNMKTARCRAALPLLKTAKRVILLSGTPAMSRPAELYTQIQAVRPALFPRFHDFGTRYCDAKQLPWGWDYSSSSNLTELKLLLEESLMLRRLKSEVLSQLPAKQRKVVTVTTDGINSRTKAALNAAARELAKGYHNKSQEKEALLVFFNHTAEAKIRAIMEYISDMLECGREKFLVFAHHKLVLDSITKELGEKSISFIRIDGSTPSAERQLLCERFQASQQSCVAVLSITAANMGLTLHSAALVVFAELFWNPGVLIQAEDRVHRIGQTSNVDIHYLVAKGTADDYLWPMIQAKMNVLEQVGLSESNISENAESASFHSRDRQQLTITEMFQRSFDEDEMLALMDQDP

Target ORF information:

RefSeq Version	NM_001127466.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127466.1

ORF Insert Sequence:

ATGTCTGTGA GTCTGACTCC AGAACAGCAG CGGAGGATTG AAGAGAACAG AAAGAAAGCA 
TTAGCCAGAA GAGCTGAAAG ACAGGCTCAG ATCGCAGATC CTGGACCTGC TCAAAACAAA 
CACACACAGT CCGGGACAAC AGGAGGCCCA GCCAAAAACA ACCAGCATTT TGCGTCTGAT 
CGGGGACAGT CAGGGGCACC TACAAGACAG ACTCAGCTCA TAGACATTAA TGCAGCGTGT 
ACAAAAACAG CACCTCCGAC TTCCATTACT GCTGCCTCTA CAGCTTCTAG TTTTTATGGA 
CAGGCAGGAA AACCAAGCAC TGGAGAAAAG AGACCACCTA AACCCCCTGA GCCCGCGGCA 
AATATCCCTG CAAAGAAACC AGCGGTGTAC TTGAGGGGAA GATGTGTTTC ACACTCAGAA 
AACCGCTTCC GAGTGGAGGT GGGCTACCAT GCAGACCTCA TCCTAGTGTT CAAAAGCATT 
CCCTCCAAAA ACTACGATCC TGCTACGAAG ATGTGGAACT TCAGCCTTGA GGACTACCAG 
ATGCTCATGG AGCAGGTGGC ACATTTACCC TCCATCTCTC TCAAACCTCT GGAAGGGATG 
GAAGGGCTGA ATATTTCTGC ATCCACATGT CGGCCCAAAG ACGCTGCAGC AATGGCGGCT 
TTGATGCGCC TCTGTCAGGG TTGGCAGAAA CCGGGTGCTA CAATTAAAGG AAAGTGCGTT 
TTAGTGTCTC GCTCTCGACT GGAGGTGGAT ATTGGATACC AGGCTGATGT TATTGGGATT 
TTTAAGCAAA TGCCATCCAA AAGTTATGAC ATGAAGACCA GAAAGTGGAC CTTTCTCCTT 
GAGGACTATG GGAAACTCAT GGCGGATCTG AATGAACTGC CTACAGTGGA GACTGAGCCT 
TTACCTCACG CCGTCCTCCA GTCCTTCTCC AGTCAGTTTG AAAAAACCCA ATCCCAAGCT 
CCAGTCCCTC CTGAAGCAGA TCTCTCACAC ATCGATCCAC AACTCACACG CAGCCTCATG 
CCTTTCCAGA GAGACGGAGT CAATTTTGCA GTGTCCAGAG AGGGTCGTTT GCTCCTGGCT 
GATGATATGG GCCTCGGGAA GACGGTTCAG GCCATCTGCA TTGCTGCCTA TTACAGGAGT 
GAGTGGCCTC TGCTGGTGGT CGCCCCGTCC TCAGTGCGCT TCACATGGGC TGAGGCTTTC 
CGCCGATGGC TGCCTTCAGT GAAGCCAGAC AGCATTAATG TGGTGGTTAA AGGAAAAGAC 
AGCCTGCGCT CCGGCCTCAT CAACATCATC AGCTATGACC TGCTCAACAA GATGGACAAA 
CAGCCGCCGT CCAGCCCATT CAACGTCATC ATCATGGATG AGTCTCACTT CCTGAAGAAC 
ATGAAAACGG CTCGCTGTCG GGCGGCTCTT CCTCTGCTCA AGACAGCTAA GAGAGTGATC 
CTGCTGTCAG GAACGCCAGC CATGTCCAGA CCCGCTGAAC TCTACACTCA GATTCAGGCT 
GTCCGGCCCG CACTGTTTCC ACGCTTTCAT GACTTCGGCA CACGATACTG CGATGCCAAA 
CAGCTGCCGT GGGGTTGGGA TTACTCCAGC TCCTCTAATC TGACTGAACT CAAGCTGCTG 
CTAGAGGAGT CTCTGATGCT CCGACGCCTC AAATCAGAGG TGCTTTCACA GCTGCCTGCC 
AAACAGAGGA AAGTGGTCAC TGTAACCACA GACGGCATCA ACTCCAGGAC CAAAGCAGCC 
CTCAATGCCG CGGCCAGAGA GCTCGCCAAA GGATACCACA ACAAATCCCA GGAGAAAGAA 
GCACTCCTGG TTTTCTTCAA CCACACAGCA GAGGCCAAGA TCAGAGCTAT CATGGAGTAT 
ATCTCAGACA TGTTGGAGTG CGGACGAGAG AAGTTTCTGG TGTTTGCTCA TCATAAACTG 
GTGCTGGACA GTATAACCAA AGAACTTGGG GAGAAGAGCA TCAGCTTCAT CCGTATCGAC 
GGCTCGACAC CGTCAGCGGA GCGTCAGCTG CTGTGTGAGC GTTTCCAGGC GTCCCAGCAG 
AGCTGTGTGG CCGTTCTGTC CATCACCGCC GCTAACATGG GGCTCACACT GCACTCTGCA 
GCACTCGTGG TGTTCGCGGA GCTCTTTTGG AACCCTGGAG TCTTGATTCA GGCTGAGGAT 
CGGGTTCACA GAATCGGTCA GACCAGTAAT GTGGACATTC ATTACCTGGT GGCCAAAGGC 
ACAGCGGACG ATTACCTGTG GCCGATGATA CAAGCCAAAA TGAATGTTCT GGAACAGGTC 
GGCTTGTCCG AATCCAACAT TTCAGAAAAT GCAGAATCTG CCAGCTTCCA CAGCAGGGAT 
CGTCAGCAGC TCACCATCAC TGAGATGTTC CAGAGGTCTT TCGATGAGGA CGAGATGCTC 
GCTTTGATGG ATCAGGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smarcal1 ( XM_009304988.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303263.1 Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43286
Clone ID Related Accession (Same CDS sequence)	XM_009304988.2
Accession Version	XM_009304988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGA GTCTGACTCC AGAACAGCAG CGGAGGATTG AAGAGAACAG AAAGAGAGCA 
TTAGCCAGAA GAGCTGAAAG ACAGGCTCAG ATCGCAGATC CTGGACCTGC TCAAAACAAA 
CACACACAGT CCGGGACAAC AGGAGGCCCA GCCAAAAACA ACCAGCATTT TGCGTCTGAT 
CGGGGACAGT CAGGGGCACC TACAAGACAG ACTCAGCTCA TAGACATTAA TGCAGCGTGT 
ACAAAAACAG CACCTCCGAC TTCCATTACT GCTGCCTCTA CAGCTTCTAG TTTTTATGGA 
CAGGCAGGAA AACCAAGCAC TGGAGAAAAG AGACCACCTA AACCCCCTGA GCCCGCGGCA 
AATATCCCTG CAAAGAAACC AGCGGTGTAC TTGAGGGGAA GATGTGTTTC ACACTCAGAA 
AACCGCTTCC GAGTGGAGGT GGGCTACCAT GCAGACCTCA TCCTAGTGTT CAAAAGCATT 
CCCTCCAAAA ACTACGATCC TGCTACGAAG ATGTGGAACT TCAGCCTTGA GGACTACCAG 
ATGCTCATGG AGCAGGTGGC ACATTTACCC TCCATCTCTC TCAAACCTCT GGAAGGGATG 
GAAGGGCTGA ATATTTCTGC ATCCACATGT CGGCCCAAAG ACGCTGCAGC AATGGCGGCT 
TTGATGCGCC TCTGTCAGGG TTGGCAGAAA CCGGGTGCTA CAATTAAAGG AAAGTGCGTT 
TTAGTGTCTC GCTCTCGACT GGAGGTGGAT ATTGGATACC AGGCTGATGT TATTGGGATT 
TTTAAGCAAA TGCCATCCAA AAGTTATGAC ATGAAGACCA GAAAGTGGAC CTTTCTCCTT 
GAGGACTATG GGAAACTCAT GGCGGATCTG AATGAACTGC CTACAGTGGA GACTGAGCCT 
TTACCTCACG CCGTCCTCCA GTCCTTCTCC AGTCAGTTTG AAAAAACCGA ATCCCGAGCT 
CCAGTCCCTC CTGAAGCAGA TCTCTCACAC ATCGATCCAC AACTCACACG CAGCCTCATG 
CCTTTCCAGA GAGACGGAGT CAATTTTGCA GTGTCCAGAG AGGGTCGTTT GCTCCTGGCT 
GATGATATGG GCCTCGGGAA GACGGTTCAG GCCATCTGCA TTGCTGCCTA TTACAGAAGT 
GAGTGGCCTC TGCTGGTGGT CGCCCCGTCC TCAGTGCGCT TCACATGGGC TGAGGCTTTC 
CGCCGATGGC TGCCTTCAGT GAAGCCAGAC AGCATTAATG TGGTGGTTAA AGGAAAAGAC 
AACCTACGCT CCGGCCTCAT CAACATCATC AGCTATGACC TGCTCAACAA GATGGACAAA 
CAGCCGCCGT CCAGCCCGTT CAACGTCATC ATCATGGATG AGTCTCACTT CCTGAAGAAC 
ATGAAAACGG CTCGCTGTCG GGCGGCTCTT CCTCTGCTCA AGACAGCTAA GAGAGTGATC 
CTGCTGTCAG GAACGCCAGC CATGTCCAGA CCCGCTGAAC TCTACACTCA GATTCAGGCT 
GTCCGGCCCG CACTGTTTCC ACGCTTTCAT GACTTCGGCA CACGATACTG CGATGCCAAA 
CAGCTGCCGT GGGGTTGGGA TTACTCCAGC TCCTCTAATC TGACTGAACT CAAGCTGCTG 
CTGGAGGAGT CTCTGATGCT CCGACGCCTC AAATCAGAGG TGCTTTCACA GCTGCCTGCC 
AAACAGAGGA AAGTGGTCAC TGTAACCACA GACGGCATCA ACTCCAGGAC CAAAGCAGCC 
CTCAATGCCG CGGCCAGAGA GCTCGCCAAA GGATACCACA ACAAATCCCA GGAGAAAGAA 
GCACTCCTGG TTTTCTTCAA CCACACAGCA GAGGCCAAGA TCAGAGCTAT CATGGAGTAT 
ATCTCAGACA TGTTGGAGTG CGGACGAGAG AAGTTTCTGG TGTTTGCTCA TCATAAACTG 
GTGCTGGACA GTATAACCAA AGAACTTGGG GAGAAGAGCA TCAGCTTCAT CCGTATCGAC 
GGCTCGACAC CGTCAGCGGA GCGTCAGCTG CTGTGTGAGC GTTTCCAGGC GTCCCAGCAG 
AGCTGTGTGG CCGTTCTGTC CATCACCGCC GCTAACATGG GGCTCACACT GCACTCTGCA 
GCACTCGTGG TGTTCGCGGA GCTCTTTTGG AACCCTGGAG TCTTGATTCA GGCTGAGGAT 
CGGGTTCACA GAATCGGTCA GACCAGTAAT GTGGACATTC ATTACCTGGT GGCCAAAGGC 
ACAGCGGACG ATTACCTGTG GCCGATGATA CAAGCCAAAA TGAATGTTCT GGAACAGGTC 
GGCTTGTCCG AATCCAACAT TTCAGAAAAT GCAGAATCTG CCAGCTTCCA CAGCAGGGAT 
CGTCAGCAGC TCACCATCAC TGAGATGTTC CAGAGGTCTT TCGATGAGGA CGAGATGCTC 
GCTTTGATGG ATCAGGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303263.1
CDS	563..2986
Translation	MSVSLTPEQQRRIEENRKRALARRAERQAQIADPGPAQNKHTQSGTTGGPAKNNQHFASDRGQSGAPTRQTQLIDINAACTKTAPPTSITAASTASSFYGQAGKPSTGEKRPPKPPEPAANIPAKKPAVYLRGRCVSHSENRFRVEVGYHADLILVFKSIPSKNYDPATKMWNFSLEDYQMLMEQVAHLPSISLKPLEGMEGLNISASTCRPKDAAAMAALMRLCQGWQKPGATIKGKCVLVSRSRLEVDIGYQADVIGIFKQMPSKSYDMKTRKWTFLLEDYGKLMADLNELPTVETEPLPHAVLQSFSSQFEKTESRAPVPPEADLSHIDPQLTRSLMPFQRDGVNFAVSREGRLLLADDMGLGKTVQAICIAAYYRSEWPLLVVAPSSVRFTWAEAFRRWLPSVKPDSINVVVKGKDNLRSGLINIISYDLLNKMDKQPPSSPFNVIIMDESHFLKNMKTARCRAALPLLKTAKRVILLSGTPAMSRPAELYTQIQAVRPALFPRFHDFGTRYCDAKQLPWGWDYSSSSNLTELKLLLEESLMLRRLKSEVLSQLPAKQRKVVTVTTDGINSRTKAALNAAARELAKGYHNKSQEKEALLVFFNHTAEAKIRAIMEYISDMLECGREKFLVFAHHKLVLDSITKELGEKSISFIRIDGSTPSAERQLLCERFQASQQSCVAVLSITAANMGLTLHSAALVVFAELFWNPGVLIQAEDRVHRIGQTSNVDIHYLVAKGTADDYLWPMIQAKMNVLEQVGLSESNISENAESASFHSRDRQQLTITEMFQRSFDEDEMLALMDQDP

Target ORF information:

RefSeq Version	XM_009304988.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304988.2

ORF Insert Sequence:

ATGTCTGTGA GTCTGACTCC AGAACAGCAG CGGAGGATTG AAGAGAACAG AAAGAGAGCA 
TTAGCCAGAA GAGCTGAAAG ACAGGCTCAG ATCGCAGATC CTGGACCTGC TCAAAACAAA 
CACACACAGT CCGGGACAAC AGGAGGCCCA GCCAAAAACA ACCAGCATTT TGCGTCTGAT 
CGGGGACAGT CAGGGGCACC TACAAGACAG ACTCAGCTCA TAGACATTAA TGCAGCGTGT 
ACAAAAACAG CACCTCCGAC TTCCATTACT GCTGCCTCTA CAGCTTCTAG TTTTTATGGA 
CAGGCAGGAA AACCAAGCAC TGGAGAAAAG AGACCACCTA AACCCCCTGA GCCCGCGGCA 
AATATCCCTG CAAAGAAACC AGCGGTGTAC TTGAGGGGAA GATGTGTTTC ACACTCAGAA 
AACCGCTTCC GAGTGGAGGT GGGCTACCAT GCAGACCTCA TCCTAGTGTT CAAAAGCATT 
CCCTCCAAAA ACTACGATCC TGCTACGAAG ATGTGGAACT TCAGCCTTGA GGACTACCAG 
ATGCTCATGG AGCAGGTGGC ACATTTACCC TCCATCTCTC TCAAACCTCT GGAAGGGATG 
GAAGGGCTGA ATATTTCTGC ATCCACATGT CGGCCCAAAG ACGCTGCAGC AATGGCGGCT 
TTGATGCGCC TCTGTCAGGG TTGGCAGAAA CCGGGTGCTA CAATTAAAGG AAAGTGCGTT 
TTAGTGTCTC GCTCTCGACT GGAGGTGGAT ATTGGATACC AGGCTGATGT TATTGGGATT 
TTTAAGCAAA TGCCATCCAA AAGTTATGAC ATGAAGACCA GAAAGTGGAC CTTTCTCCTT 
GAGGACTATG GGAAACTCAT GGCGGATCTG AATGAACTGC CTACAGTGGA GACTGAGCCT 
TTACCTCACG CCGTCCTCCA GTCCTTCTCC AGTCAGTTTG AAAAAACCGA ATCCCGAGCT 
CCAGTCCCTC CTGAAGCAGA TCTCTCACAC ATCGATCCAC AACTCACACG CAGCCTCATG 
CCTTTCCAGA GAGACGGAGT CAATTTTGCA GTGTCCAGAG AGGGTCGTTT GCTCCTGGCT 
GATGATATGG GCCTCGGGAA GACGGTTCAG GCCATCTGCA TTGCTGCCTA TTACAGAAGT 
GAGTGGCCTC TGCTGGTGGT CGCCCCGTCC TCAGTGCGCT TCACATGGGC TGAGGCTTTC 
CGCCGATGGC TGCCTTCAGT GAAGCCAGAC AGCATTAATG TGGTGGTTAA AGGAAAAGAC 
AACCTACGCT CCGGCCTCAT CAACATCATC AGCTATGACC TGCTCAACAA GATGGACAAA 
CAGCCGCCGT CCAGCCCGTT CAACGTCATC ATCATGGATG AGTCTCACTT CCTGAAGAAC 
ATGAAAACGG CTCGCTGTCG GGCGGCTCTT CCTCTGCTCA AGACAGCTAA GAGAGTGATC 
CTGCTGTCAG GAACGCCAGC CATGTCCAGA CCCGCTGAAC TCTACACTCA GATTCAGGCT 
GTCCGGCCCG CACTGTTTCC ACGCTTTCAT GACTTCGGCA CACGATACTG CGATGCCAAA 
CAGCTGCCGT GGGGTTGGGA TTACTCCAGC TCCTCTAATC TGACTGAACT CAAGCTGCTG 
CTGGAGGAGT CTCTGATGCT CCGACGCCTC AAATCAGAGG TGCTTTCACA GCTGCCTGCC 
AAACAGAGGA AAGTGGTCAC TGTAACCACA GACGGCATCA ACTCCAGGAC CAAAGCAGCC 
CTCAATGCCG CGGCCAGAGA GCTCGCCAAA GGATACCACA ACAAATCCCA GGAGAAAGAA 
GCACTCCTGG TTTTCTTCAA CCACACAGCA GAGGCCAAGA TCAGAGCTAT CATGGAGTAT 
ATCTCAGACA TGTTGGAGTG CGGACGAGAG AAGTTTCTGG TGTTTGCTCA TCATAAACTG 
GTGCTGGACA GTATAACCAA AGAACTTGGG GAGAAGAGCA TCAGCTTCAT CCGTATCGAC 
GGCTCGACAC CGTCAGCGGA GCGTCAGCTG CTGTGTGAGC GTTTCCAGGC GTCCCAGCAG 
AGCTGTGTGG CCGTTCTGTC CATCACCGCC GCTAACATGG GGCTCACACT GCACTCTGCA 
GCACTCGTGG TGTTCGCGGA GCTCTTTTGG AACCCTGGAG TCTTGATTCA GGCTGAGGAT 
CGGGTTCACA GAATCGGTCA GACCAGTAAT GTGGACATTC ATTACCTGGT GGCCAAAGGC 
ACAGCGGACG ATTACCTGTG GCCGATGATA CAAGCCAAAA TGAATGTTCT GGAACAGGTC 
GGCTTGTCCG AATCCAACAT TTCAGAAAAT GCAGAATCTG CCAGCTTCCA CAGCAGGGAT 
CGTCAGCAGC TCACCATCAC TGAGATGTTC CAGAGGTCTT TCGATGAGGA CGAGATGCTC 
GCTTTGATGG ATCAGGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smarcal1 ( XM_009304989.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303264.1 Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43287
Clone ID Related Accession (Same CDS sequence)	XM_009304989.2 , XM_021478691.1
Accession Version	XM_009304989.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304989.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGA GTCTGACTCC AGAACAGCAG CGGAGGATTG AAGAGAACAG AAAGAGAGCA 
TTAGCCAGAA GAGCTGAAAG ACAGGCTCAG ATCGCAGATC CTGGACCTGC TCAAAACAAA 
CACACACAGT CCGGGACAAC AGGAGGCCCA GCCAAAAACA ACCAGCATTT TGCGTCTGAT 
CGGGGACAGT CAGGGGCACC TACAAGACAG ACTCAGCTCA TAGACATTAA TGCAGCGTGT 
ACAAAAACAG CACCTCCGAC TTCCATTACT GCTGCCTCTA CAGCTTCTAG TTTTTATGGA 
CAGGCAGGAA AACCAAGCAC TGGAGAAAAG AGACCACCTA AACCCCCTGA GCCCGCGGCA 
AATATCCCTG CAAAGAAACC AGCGGTGTAC TTGAGGGGAA GATGTGTTTC ACACTCAGAA 
AACCGCTTCC GAGTGGAGGT GGGCTACCAT GCAGACCTCA TCCTAGTGTT CAAAAGCATT 
CCCTCCAAAA ACTACGATCC TGCTACGAAG ATGTGGAACT TCAGCCTTGA GGACTACCAG 
ATGCTCATGG AGCAGGTGGC ACATTTACCC TCCATCTCTC TCAAACCTCT GGAAGGGATG 
GAAGGGCTGA ATATTTCTGC ATCCACATGT CGGCCCAAAG ACGCTGCAGC AATGGCGGCT 
TTGATGCGCC TCTGTCAGGG TTGGCAGAAA CCGGGTGCTA CAATTAAAGG AAAGTGCGTT 
TTAGTGTCTC GCTCTCGACT GGAGGTGGAT ATTGGATACC AGGCTGATGT TATTGGGATT 
TTTAAGCAAA TGCCATCCAA AAGTTATGAC ATGAAGACCA GAAAGTGGAC CTTTCTCCTT 
GAGGACTATG GGAAACTCAT GGCGGATCTG AATGAACTGC CTACAGTGGA GACTGAGCCT 
TTACCTCACG CCGTCCTCCA GTCCTTCTCC AGTCAGTTTG AAAAAACCGA ATCCCGAGCT 
CCAGTCCCTC CTGAAGCAGA TCTCTCACAC ATCGATCCAC AACTCACACG CAGCCTCATG 
CCTTTCCAGA GAGACGGAGT CAATTTTGCA GTGTCCAGAG AGGGTCGTTT GCTCCTGGCT 
GATGATATGG GCCTCGGGAA GACGGTTCAG GCCATCTGCA TTGCTGCCTA TTACAGAAGT 
GAGTGGCCTC TGCTGGTGGT CGCCCCGTCC TCAGTGCGCT TCACATGGGC TGAGGCTTTC 
CGCCGATGGC TGCCTTCAGT GAAGCCAGAC AGCATTAATG TGGTGGTTAA AGGAAAAGAC 
AACCTACGCT CCGGCCTCAT CAACATCATC AGCTATGACC TGCTCAACAA GATGGACAAA 
CAGCCGCCGT CCAGCCCGTT CAACGTCATC ATCATGGATG AGTCTCACTT CCTGAAGAAC 
ATGAAAACGG CTCGCTGTCG GGCGGCTCTT CCTCTGCTCA AGACAGCTAA GAGAGTGATC 
CTGCTGTCAG GAACGCCAGC CATGTCCAGA CCCGCTGAAC TCTACACTCA GATTCAGGCT 
GTCCGGCCCG CACTGTTTCC ACGCTTTCAT GACTTCGGCA CACGATACTG CGATGCCAAA 
CAGCTGCCGT GGGGTTGGGA TTACTCCAGC TCCTCTAATC TGACTGAACT CAAGCTGCTG 
CTGGAGGAGT CTCTGATGCT CCGACGCCTC AAATCAGAGG TGCTTTCACA GCTGCCTGCC 
AAACAGAGGA AAGTGGTCAC TGTAACCACA GACGGCATCA ACTCCAGGAC CAAAGCAGCC 
CTCAATGCCG CGGCCAGAGA GCTCGCCAAA GGATACCACA ACAAATCCCA GGAGAAAGAA 
GCACTCCTGG TTTTCTTCAA CCACACAGCA GAGGCCAAGA TCAGAGCTAT CATGGAGTAT 
ATCTCAGACA TGTTGGAGTG CGGACGAGAG AAGTTTCTGG TGTTTGCTCA TCATAAACTG 
GTGCTGGACA GTATAACCAA AGAACTTGGG GAGAAGAGCA TCAGCTTCAT CCGTATCGAC 
GGCTCGACAC CGTCAGCGGA GCGTCAGCTG CTGTGTGAGC GTTTCCAGGC GTCCCAGCAG 
AGCTGTGTGG CCGTTCTGTC CATCACCGCC GCTAACATGG GGCTCACACT GCACTCTGCA 
GCACTCGTGG TGTTCGCGGA GCTCTTTTGG AACCCTGGAG TCTTGATTCA GGCTGAGGAT 
CGGGTTCACA GAATCGGTCA GACCAGTAAT GTGGACATTC ATTACCTGGT GGCCAAAGGC 
ACAGCGGACG ATTACCTGTG GCCGATGATA CAAGCCAAAA TGAATGTTCT GGAACAGGTC 
GGCTTGTCCG AATCCAACAT TTCAGAAAAT GCAGAATCTG CCAGCTTCCA CAGCAGGGAT 
CGTCAGCAGC TCACCATCAC TGAGATGTTC CAGAGGTCTT TCGATGAGGA CGAGATGCTC 
GCTTTGATGG ATCAGGACCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303264.1
CDS	566..2989
Translation	MSVSLTPEQQRRIEENRKRALARRAERQAQIADPGPAQNKHTQSGTTGGPAKNNQHFASDRGQSGAPTRQTQLIDINAACTKTAPPTSITAASTASSFYGQAGKPSTGEKRPPKPPEPAANIPAKKPAVYLRGRCVSHSENRFRVEVGYHADLILVFKSIPSKNYDPATKMWNFSLEDYQMLMEQVAHLPSISLKPLEGMEGLNISASTCRPKDAAAMAALMRLCQGWQKPGATIKGKCVLVSRSRLEVDIGYQADVIGIFKQMPSKSYDMKTRKWTFLLEDYGKLMADLNELPTVETEPLPHAVLQSFSSQFEKTESRAPVPPEADLSHIDPQLTRSLMPFQRDGVNFAVSREGRLLLADDMGLGKTVQAICIAAYYRSEWPLLVVAPSSVRFTWAEAFRRWLPSVKPDSINVVVKGKDNLRSGLINIISYDLLNKMDKQPPSSPFNVIIMDESHFLKNMKTARCRAALPLLKTAKRVILLSGTPAMSRPAELYTQIQAVRPALFPRFHDFGTRYCDAKQLPWGWDYSSSSNLTELKLLLEESLMLRRLKSEVLSQLPAKQRKVVTVTTDGINSRTKAALNAAARELAKGYHNKSQEKEALLVFFNHTAEAKIRAIMEYISDMLECGREKFLVFAHHKLVLDSITKELGEKSISFIRIDGSTPSAERQLLCERFQASQQSCVAVLSITAANMGLTLHSAALVVFAELFWNPGVLIQAEDRVHRIGQTSNVDIHYLVAKGTADDYLWPMIQAKMNVLEQVGLSESNISENAESASFHSRDRQQLTITEMFQRSFDEDEMLALMDQDP

Target ORF information:

RefSeq Version	XM_009304989.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304989.2

ORF Insert Sequence:

ATGTCTGTGA GTCTGACTCC AGAACAGCAG CGGAGGATTG AAGAGAACAG AAAGAGAGCA 
TTAGCCAGAA GAGCTGAAAG ACAGGCTCAG ATCGCAGATC CTGGACCTGC TCAAAACAAA 
CACACACAGT CCGGGACAAC AGGAGGCCCA GCCAAAAACA ACCAGCATTT TGCGTCTGAT 
CGGGGACAGT CAGGGGCACC TACAAGACAG ACTCAGCTCA TAGACATTAA TGCAGCGTGT 
ACAAAAACAG CACCTCCGAC TTCCATTACT GCTGCCTCTA CAGCTTCTAG TTTTTATGGA 
CAGGCAGGAA AACCAAGCAC TGGAGAAAAG AGACCACCTA AACCCCCTGA GCCCGCGGCA 
AATATCCCTG CAAAGAAACC AGCGGTGTAC TTGAGGGGAA GATGTGTTTC ACACTCAGAA 
AACCGCTTCC GAGTGGAGGT GGGCTACCAT GCAGACCTCA TCCTAGTGTT CAAAAGCATT 
CCCTCCAAAA ACTACGATCC TGCTACGAAG ATGTGGAACT TCAGCCTTGA GGACTACCAG 
ATGCTCATGG AGCAGGTGGC ACATTTACCC TCCATCTCTC TCAAACCTCT GGAAGGGATG 
GAAGGGCTGA ATATTTCTGC ATCCACATGT CGGCCCAAAG ACGCTGCAGC AATGGCGGCT 
TTGATGCGCC TCTGTCAGGG TTGGCAGAAA CCGGGTGCTA CAATTAAAGG AAAGTGCGTT 
TTAGTGTCTC GCTCTCGACT GGAGGTGGAT ATTGGATACC AGGCTGATGT TATTGGGATT 
TTTAAGCAAA TGCCATCCAA AAGTTATGAC ATGAAGACCA GAAAGTGGAC CTTTCTCCTT 
GAGGACTATG GGAAACTCAT GGCGGATCTG AATGAACTGC CTACAGTGGA GACTGAGCCT 
TTACCTCACG CCGTCCTCCA GTCCTTCTCC AGTCAGTTTG AAAAAACCGA ATCCCGAGCT 
CCAGTCCCTC CTGAAGCAGA TCTCTCACAC ATCGATCCAC AACTCACACG CAGCCTCATG 
CCTTTCCAGA GAGACGGAGT CAATTTTGCA GTGTCCAGAG AGGGTCGTTT GCTCCTGGCT 
GATGATATGG GCCTCGGGAA GACGGTTCAG GCCATCTGCA TTGCTGCCTA TTACAGAAGT 
GAGTGGCCTC TGCTGGTGGT CGCCCCGTCC TCAGTGCGCT TCACATGGGC TGAGGCTTTC 
CGCCGATGGC TGCCTTCAGT GAAGCCAGAC AGCATTAATG TGGTGGTTAA AGGAAAAGAC 
AACCTACGCT CCGGCCTCAT CAACATCATC AGCTATGACC TGCTCAACAA GATGGACAAA 
CAGCCGCCGT CCAGCCCGTT CAACGTCATC ATCATGGATG AGTCTCACTT CCTGAAGAAC 
ATGAAAACGG CTCGCTGTCG GGCGGCTCTT CCTCTGCTCA AGACAGCTAA GAGAGTGATC 
CTGCTGTCAG GAACGCCAGC CATGTCCAGA CCCGCTGAAC TCTACACTCA GATTCAGGCT 
GTCCGGCCCG CACTGTTTCC ACGCTTTCAT GACTTCGGCA CACGATACTG CGATGCCAAA 
CAGCTGCCGT GGGGTTGGGA TTACTCCAGC TCCTCTAATC TGACTGAACT CAAGCTGCTG 
CTGGAGGAGT CTCTGATGCT CCGACGCCTC AAATCAGAGG TGCTTTCACA GCTGCCTGCC 
AAACAGAGGA AAGTGGTCAC TGTAACCACA GACGGCATCA ACTCCAGGAC CAAAGCAGCC 
CTCAATGCCG CGGCCAGAGA GCTCGCCAAA GGATACCACA ACAAATCCCA GGAGAAAGAA 
GCACTCCTGG TTTTCTTCAA CCACACAGCA GAGGCCAAGA TCAGAGCTAT CATGGAGTAT 
ATCTCAGACA TGTTGGAGTG CGGACGAGAG AAGTTTCTGG TGTTTGCTCA TCATAAACTG 
GTGCTGGACA GTATAACCAA AGAACTTGGG GAGAAGAGCA TCAGCTTCAT CCGTATCGAC 
GGCTCGACAC CGTCAGCGGA GCGTCAGCTG CTGTGTGAGC GTTTCCAGGC GTCCCAGCAG 
AGCTGTGTGG CCGTTCTGTC CATCACCGCC GCTAACATGG GGCTCACACT GCACTCTGCA 
GCACTCGTGG TGTTCGCGGA GCTCTTTTGG AACCCTGGAG TCTTGATTCA GGCTGAGGAT 
CGGGTTCACA GAATCGGTCA GACCAGTAAT GTGGACATTC ATTACCTGGT GGCCAAAGGC 
ACAGCGGACG ATTACCTGTG GCCGATGATA CAAGCCAAAA TGAATGTTCT GGAACAGGTC 
GGCTTGTCCG AATCCAACAT TTCAGAAAAT GCAGAATCTG CCAGCTTCCA CAGCAGGGAT 
CGTCAGCAGC TCACCATCAC TGAGATGTTC CAGAGGTCTT TCGATGAGGA CGAGATGCTC 
GCTTTGATGG ATCAGGACCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smarcal1 ( XM_021478691.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334366.1 Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43287
Clone ID Related Accession (Same CDS sequence)	XM_009304989.2 , XM_021478691.1
Accession Version	XM_021478691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGA GTCTGACTCC AGAACAGCAG CGGAGGATTG AAGAGAACAG AAAGAGAGCA 
TTAGCCAGAA GAGCTGAAAG ACAGGCTCAG ATCGCAGATC CTGGACCTGC TCAAAACAAA 
CACACACAGT CCGGGACAAC AGGAGGCCCA GCCAAAAACA ACCAGCATTT TGCGTCTGAT 
CGGGGACAGT CAGGGGCACC TACAAGACAG ACTCAGCTCA TAGACATTAA TGCAGCGTGT 
ACAAAAACAG CACCTCCGAC TTCCATTACT GCTGCCTCTA CAGCTTCTAG TTTTTATGGA 
CAGGCAGGAA AACCAAGCAC TGGAGAAAAG AGACCACCTA AACCCCCTGA GCCCGCGGCA 
AATATCCCTG CAAAGAAACC AGCGGTGTAC TTGAGGGGAA GATGTGTTTC ACACTCAGAA 
AACCGCTTCC GAGTGGAGGT GGGCTACCAT GCAGACCTCA TCCTAGTGTT CAAAAGCATT 
CCCTCCAAAA ACTACGATCC TGCTACGAAG ATGTGGAACT TCAGCCTTGA GGACTACCAG 
ATGCTCATGG AGCAGGTGGC ACATTTACCC TCCATCTCTC TCAAACCTCT GGAAGGGATG 
GAAGGGCTGA ATATTTCTGC ATCCACATGT CGGCCCAAAG ACGCTGCAGC AATGGCGGCT 
TTGATGCGCC TCTGTCAGGG TTGGCAGAAA CCGGGTGCTA CAATTAAAGG AAAGTGCGTT 
TTAGTGTCTC GCTCTCGACT GGAGGTGGAT ATTGGATACC AGGCTGATGT TATTGGGATT 
TTTAAGCAAA TGCCATCCAA AAGTTATGAC ATGAAGACCA GAAAGTGGAC CTTTCTCCTT 
GAGGACTATG GGAAACTCAT GGCGGATCTG AATGAACTGC CTACAGTGGA GACTGAGCCT 
TTACCTCACG CCGTCCTCCA GTCCTTCTCC AGTCAGTTTG AAAAAACCGA ATCCCGAGCT 
CCAGTCCCTC CTGAAGCAGA TCTCTCACAC ATCGATCCAC AACTCACACG CAGCCTCATG 
CCTTTCCAGA GAGACGGAGT CAATTTTGCA GTGTCCAGAG AGGGTCGTTT GCTCCTGGCT 
GATGATATGG GCCTCGGGAA GACGGTTCAG GCCATCTGCA TTGCTGCCTA TTACAGAAGT 
GAGTGGCCTC TGCTGGTGGT CGCCCCGTCC TCAGTGCGCT TCACATGGGC TGAGGCTTTC 
CGCCGATGGC TGCCTTCAGT GAAGCCAGAC AGCATTAATG TGGTGGTTAA AGGAAAAGAC 
AACCTACGCT CCGGCCTCAT CAACATCATC AGCTATGACC TGCTCAACAA GATGGACAAA 
CAGCCGCCGT CCAGCCCGTT CAACGTCATC ATCATGGATG AGTCTCACTT CCTGAAGAAC 
ATGAAAACGG CTCGCTGTCG GGCGGCTCTT CCTCTGCTCA AGACAGCTAA GAGAGTGATC 
CTGCTGTCAG GAACGCCAGC CATGTCCAGA CCCGCTGAAC TCTACACTCA GATTCAGGCT 
GTCCGGCCCG CACTGTTTCC ACGCTTTCAT GACTTCGGCA CACGATACTG CGATGCCAAA 
CAGCTGCCGT GGGGTTGGGA TTACTCCAGC TCCTCTAATC TGACTGAACT CAAGCTGCTG 
CTGGAGGAGT CTCTGATGCT CCGACGCCTC AAATCAGAGG TGCTTTCACA GCTGCCTGCC 
AAACAGAGGA AAGTGGTCAC TGTAACCACA GACGGCATCA ACTCCAGGAC CAAAGCAGCC 
CTCAATGCCG CGGCCAGAGA GCTCGCCAAA GGATACCACA ACAAATCCCA GGAGAAAGAA 
GCACTCCTGG TTTTCTTCAA CCACACAGCA GAGGCCAAGA TCAGAGCTAT CATGGAGTAT 
ATCTCAGACA TGTTGGAGTG CGGACGAGAG AAGTTTCTGG TGTTTGCTCA TCATAAACTG 
GTGCTGGACA GTATAACCAA AGAACTTGGG GAGAAGAGCA TCAGCTTCAT CCGTATCGAC 
GGCTCGACAC CGTCAGCGGA GCGTCAGCTG CTGTGTGAGC GTTTCCAGGC GTCCCAGCAG 
AGCTGTGTGG CCGTTCTGTC CATCACCGCC GCTAACATGG GGCTCACACT GCACTCTGCA 
GCACTCGTGG TGTTCGCGGA GCTCTTTTGG AACCCTGGAG TCTTGATTCA GGCTGAGGAT 
CGGGTTCACA GAATCGGTCA GACCAGTAAT GTGGACATTC ATTACCTGGT GGCCAAAGGC 
ACAGCGGACG ATTACCTGTG GCCGATGATA CAAGCCAAAA TGAATGTTCT GGAACAGGTC 
GGCTTGTCCG AATCCAACAT TTCAGAAAAT GCAGAATCTG CCAGCTTCCA CAGCAGGGAT 
CGTCAGCAGC TCACCATCAC TGAGATGTTC CAGAGGTCTT TCGATGAGGA CGAGATGCTC 
GCTTTGATGG ATCAGGACCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334366.1
CDS	565..2988
Translation	MSVSLTPEQQRRIEENRKRALARRAERQAQIADPGPAQNKHTQSGTTGGPAKNNQHFASDRGQSGAPTRQTQLIDINAACTKTAPPTSITAASTASSFYGQAGKPSTGEKRPPKPPEPAANIPAKKPAVYLRGRCVSHSENRFRVEVGYHADLILVFKSIPSKNYDPATKMWNFSLEDYQMLMEQVAHLPSISLKPLEGMEGLNISASTCRPKDAAAMAALMRLCQGWQKPGATIKGKCVLVSRSRLEVDIGYQADVIGIFKQMPSKSYDMKTRKWTFLLEDYGKLMADLNELPTVETEPLPHAVLQSFSSQFEKTESRAPVPPEADLSHIDPQLTRSLMPFQRDGVNFAVSREGRLLLADDMGLGKTVQAICIAAYYRSEWPLLVVAPSSVRFTWAEAFRRWLPSVKPDSINVVVKGKDNLRSGLINIISYDLLNKMDKQPPSSPFNVIIMDESHFLKNMKTARCRAALPLLKTAKRVILLSGTPAMSRPAELYTQIQAVRPALFPRFHDFGTRYCDAKQLPWGWDYSSSSNLTELKLLLEESLMLRRLKSEVLSQLPAKQRKVVTVTTDGINSRTKAALNAAARELAKGYHNKSQEKEALLVFFNHTAEAKIRAIMEYISDMLECGREKFLVFAHHKLVLDSITKELGEKSISFIRIDGSTPSAERQLLCERFQASQQSCVAVLSITAANMGLTLHSAALVVFAELFWNPGVLIQAEDRVHRIGQTSNVDIHYLVAKGTADDYLWPMIQAKMNVLEQVGLSESNISENAESASFHSRDRQQLTITEMFQRSFDEDEMLALMDQDP

Target ORF information:

RefSeq Version	XM_021478691.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (smarcal1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478691.1

ORF Insert Sequence:

ATGTCTGTGA GTCTGACTCC AGAACAGCAG CGGAGGATTG AAGAGAACAG AAAGAGAGCA 
TTAGCCAGAA GAGCTGAAAG ACAGGCTCAG ATCGCAGATC CTGGACCTGC TCAAAACAAA 
CACACACAGT CCGGGACAAC AGGAGGCCCA GCCAAAAACA ACCAGCATTT TGCGTCTGAT 
CGGGGACAGT CAGGGGCACC TACAAGACAG ACTCAGCTCA TAGACATTAA TGCAGCGTGT 
ACAAAAACAG CACCTCCGAC TTCCATTACT GCTGCCTCTA CAGCTTCTAG TTTTTATGGA 
CAGGCAGGAA AACCAAGCAC TGGAGAAAAG AGACCACCTA AACCCCCTGA GCCCGCGGCA 
AATATCCCTG CAAAGAAACC AGCGGTGTAC TTGAGGGGAA GATGTGTTTC ACACTCAGAA 
AACCGCTTCC GAGTGGAGGT GGGCTACCAT GCAGACCTCA TCCTAGTGTT CAAAAGCATT 
CCCTCCAAAA ACTACGATCC TGCTACGAAG ATGTGGAACT TCAGCCTTGA GGACTACCAG 
ATGCTCATGG AGCAGGTGGC ACATTTACCC TCCATCTCTC TCAAACCTCT GGAAGGGATG 
GAAGGGCTGA ATATTTCTGC ATCCACATGT CGGCCCAAAG ACGCTGCAGC AATGGCGGCT 
TTGATGCGCC TCTGTCAGGG TTGGCAGAAA CCGGGTGCTA CAATTAAAGG AAAGTGCGTT 
TTAGTGTCTC GCTCTCGACT GGAGGTGGAT ATTGGATACC AGGCTGATGT TATTGGGATT 
TTTAAGCAAA TGCCATCCAA AAGTTATGAC ATGAAGACCA GAAAGTGGAC CTTTCTCCTT 
GAGGACTATG GGAAACTCAT GGCGGATCTG AATGAACTGC CTACAGTGGA GACTGAGCCT 
TTACCTCACG CCGTCCTCCA GTCCTTCTCC AGTCAGTTTG AAAAAACCGA ATCCCGAGCT 
CCAGTCCCTC CTGAAGCAGA TCTCTCACAC ATCGATCCAC AACTCACACG CAGCCTCATG 
CCTTTCCAGA GAGACGGAGT CAATTTTGCA GTGTCCAGAG AGGGTCGTTT GCTCCTGGCT 
GATGATATGG GCCTCGGGAA GACGGTTCAG GCCATCTGCA TTGCTGCCTA TTACAGAAGT 
GAGTGGCCTC TGCTGGTGGT CGCCCCGTCC TCAGTGCGCT TCACATGGGC TGAGGCTTTC 
CGCCGATGGC TGCCTTCAGT GAAGCCAGAC AGCATTAATG TGGTGGTTAA AGGAAAAGAC 
AACCTACGCT CCGGCCTCAT CAACATCATC AGCTATGACC TGCTCAACAA GATGGACAAA 
CAGCCGCCGT CCAGCCCGTT CAACGTCATC ATCATGGATG AGTCTCACTT CCTGAAGAAC 
ATGAAAACGG CTCGCTGTCG GGCGGCTCTT CCTCTGCTCA AGACAGCTAA GAGAGTGATC 
CTGCTGTCAG GAACGCCAGC CATGTCCAGA CCCGCTGAAC TCTACACTCA GATTCAGGCT 
GTCCGGCCCG CACTGTTTCC ACGCTTTCAT GACTTCGGCA CACGATACTG CGATGCCAAA 
CAGCTGCCGT GGGGTTGGGA TTACTCCAGC TCCTCTAATC TGACTGAACT CAAGCTGCTG 
CTGGAGGAGT CTCTGATGCT CCGACGCCTC AAATCAGAGG TGCTTTCACA GCTGCCTGCC 
AAACAGAGGA AAGTGGTCAC TGTAACCACA GACGGCATCA ACTCCAGGAC CAAAGCAGCC 
CTCAATGCCG CGGCCAGAGA GCTCGCCAAA GGATACCACA ACAAATCCCA GGAGAAAGAA 
GCACTCCTGG TTTTCTTCAA CCACACAGCA GAGGCCAAGA TCAGAGCTAT CATGGAGTAT 
ATCTCAGACA TGTTGGAGTG CGGACGAGAG AAGTTTCTGG TGTTTGCTCA TCATAAACTG 
GTGCTGGACA GTATAACCAA AGAACTTGGG GAGAAGAGCA TCAGCTTCAT CCGTATCGAC 
GGCTCGACAC CGTCAGCGGA GCGTCAGCTG CTGTGTGAGC GTTTCCAGGC GTCCCAGCAG 
AGCTGTGTGG CCGTTCTGTC CATCACCGCC GCTAACATGG GGCTCACACT GCACTCTGCA 
GCACTCGTGG TGTTCGCGGA GCTCTTTTGG AACCCTGGAG TCTTGATTCA GGCTGAGGAT 
CGGGTTCACA GAATCGGTCA GACCAGTAAT GTGGACATTC ATTACCTGGT GGCCAAAGGC 
ACAGCGGACG ATTACCTGTG GCCGATGATA CAAGCCAAAA TGAATGTTCT GGAACAGGTC 
GGCTTGTCCG AATCCAACAT TTCAGAAAAT GCAGAATCTG CCAGCTTCCA CAGCAGGGAT 
CGTCAGCAGC TCACCATCAC TGAGATGTTC CAGAGGTCTT TCGATGAGGA CGAGATGCTC 
GCTTTGATGG ATCAGGACCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Deficiency of smarcal1 causes cell cycle arrest and developmental abnormalities in zebrafish.
Developmental biology339(1)89-100(2010 Mar)
Huang C,Gu S,Yu P,Yu F,Feng C,Gao N,Du J

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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smarcal1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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