taf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	taf1
Entrez Gene ID	555452
Full Name	TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor
Synonyms	wu:fi04f03,wu:fy39f07
General protein information	Preferred Names TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor Names transcription initiation factor TFIID subunit 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021476170.1	XP_021331845.1	transcription initiation factor TFIID subunit 1 isoform X2
XM_005165316.4	XP_005165373.1	transcription initiation factor TFIID subunit 1 isoform X4
NM_001044785.1	NP_001038250.1	transcription initiation factor TFIID subunit 1
XM_017356092.1	XP_017211581.1	transcription initiation factor TFIID subunit 1 isoform X2
XM_021476169.1	XP_021331844.1	transcription initiation factor TFIID subunit 1 isoform X1
XM_005165316.3	XP_005165373.1	transcription initiation factor TFIID subunit 1 isoform X1
XM_021476171.1	XP_021331846.1	transcription initiation factor TFIID subunit 1 isoform X3

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003882	XP_308108.5
Xenopus tropicalis (tropical clawed frog)	taf1	XP_002934954.2
Canis lupus familiaris (dog)	TAF1	XP_005641531.1
Mus musculus (house mouse)	Taf1	NP_001074477.1
Danio rerio (zebrafish)	taf1	NP_001038250.1
Drosophila melanogaster (fruit fly)	Taf1	NP_996159.1
Caenorhabditis elegans (roundworm)	taf-1	NP_493426.2
Homo sapiens (human)	TAF1	NP_004597.2
Rattus norvegicus (Norway rat)	Taf1	NP_001178652.1
Bos taurus (cattle)	TAF1	XP_005228102.1
Gallus gallus (chicken)	TAF1	XP_420198.4
	TAF1	XP_002808585.1

Gene Ontology
Bibliography

Related articles in PubMed

ZNHIT3 is defective in PEHO syndrome, a severe encephalopathy with cerebellar granule neuron loss.
Anttonen AK, Laari A, Kousi M, Yang YJ, J??skel?inen T, Somer M, Siintola E, Jakkula E, Muona M, Tegelberg S, L?nnqvist T, Pihko H, Valanne L, Paetau A, Lun MP, H?stbacka J, Kopra O, Joensuu T, Katsanis N, Lehtinen MK, Palvimo JJ, Lehesjoki AE
Brain : a journal of neurology140(5)1267-1279(2017 05)

TAF1 Variants Are Associated with Dysmorphic Features, Intellectual Disability, and Neurological Manifestations.
O'Rawe JA, Wu Y, D?rfel MJ, Rope AF, Au PY, Parboosingh JS, Moon S, Kousi M, Kosma K, Smith CS, Tzetis M, Schuette JL, Hufnagel RB, Prada CE, Martinez F, Orellana C, Crain J, Caro-Llopis A, Oltra S, Monfort S, Jim?nez-Barr?n LT, Swensen J, Ellingwood S, Smith R, Fang H, Ospina S, Stegmann S, Den Hollander N, Mittelman D, Highnam G, Robison R, Yang E, Faivre L, Roubertie A, Rivi?re JB, Monaghan KG, Wang K, Davis EE, Katsanis N, Kalscheuer VM, Wang EH, Metcalfe K, Kleefstra T, Innes AM, Kitsiou-Tzeli S, Rosello M, Keegan CE, Lyon GJ
American journal of human genetics97(6)922-32(2015 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following taf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the taf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142635	XM_021476169.1 Latest version!	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142636	XM_021476170.1 Latest version!	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142637	XM_021476171.1 Latest version!	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59083	XM_017356092.1 Latest version!	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa36572	NM_001044785.1 Latest version!	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10706	XM_005165316.4 Latest version!	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa10706	XM_005165316.3	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

taf1 ( XM_021476169.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331844.1 Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142635
Clone ID Related Accession (Same CDS sequence)	XM_021476169.1
Accession Version	XM_021476169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transcription initiation factor TFIID subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGC CTGATGTGTG TGAGAACAGT 
GTGTCTGTGA GTTTGCAGGG AGACTCTAGT CTGTTAGCCG AGGCTACGCT AATCCCTCCC 
ACCCCTGAGA AAAGAGGGGG GCAGGGTCGA CACAGAGGCA GATTGGGTGA AGAGGAGTCT 
GATGTGGACA TAGAAGGATT TGAGGAAGAT GATGACGGCA AACCGAAAAC ACCTGCTCCG 
CACTTTCAGG TTGAAGATGG AGATCTGGAT GATGAGGATG ACGATGATGA AGATGATGAG 
CTCCTTATGC GGCCACCGAG GCGTATGCAC ATTGATGAAG ATGACGACGA TGATGATGAG 
GGCTCCAGTC GACCAGCACA GGCCAGTGTG CTTTACCAGG ATCTGCTGAT GTCAGATGCT 
GAAGATGATG CCAGTGAGGA GGAAGGCGAT AATCCATTCT CCTCTATCCA ACTGTCTGAG 
AGCGGCAGTG ATAGTGATGC TGACCTGGGA CATCAAGAGA GTACACGGAT TGGACTTGAA 
CAGGAGGAGA GTATGATGTC ATATGAAGGG GAGGGACCTG ACGGCTTAAC GCATATGGAG 
GACAGCAATG TCAGCTTTGG CAGCTATGAT GATGGAGACA GTCAGATGCA GAGGCAGGTG 
TCCAGCCCCA GGACGGGAGA GCGAGATGTA GATGAGGTCT ACGGGATCAG TGAGGAGGAA 
GAGGATGAGG AGGAAGAGGA TGAGCGCAGG AGAGGCCCGA GTGTCCTCAC TCAGGTACAG 
CTCAGTGACG ATGAGGAGGA CAGTGAAGAA TTCAGATCTG TCGGAGGAGA CAGCGACATG 
GACTCTGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331844.1
CDS	372..6326
Translation	MSDSDSDEDQDRPFHLTGFLFGNINENGQLEDDSVLDTESKKHLAGLGTLGLGSLITEITASEEETAEQEQDQSSTDAEGWVRSTEDAVDYSDISEVAEDETRKYRQAMGNLQPSRRTDDEDDYDADCEDVDSKLMPPPPPPTQPITAKKDDTPTQNSSVTDEGDGIILPSIIAPSSVGDKVDFSSSSDSESESDRPSQGSGTGKKAGCLTLPLAGIMQKDAAKALPGVTELFPEFRPGKVLRFLRLFGPGKNMPSVWRSARRKRKRKQKEPQSDMAAAGDIESLPSEPGAKKKSGWDYEYAPPPPPEQCLSDDEITMMAPVESKFMQVSGEGDKVSEMRPKVAEWRYGPAQLWYDMLGVPEDGSGFHYGFKLKDDKQEDNTDAVTKMATPPASEPVPEQEPPQESQDKADEDAPMQDELFLMVTQLQWEEDIIWNGEDVKHKGTKTQRASLAGWLPTSMTRNANAYNAQQGLSRSNSQLVPPTPPPLTKTPSITGSKRDKHNHDHQVSHEDDTPWFSIFPIDNEELVYGRWEDNIIWDDQNMDCIPSPPVLTLNPNDENIILEIPDEKEERTSHSPSKENKKETALKKSRILLGKTGVIKDEPQQNMSQPEIKDPWNLSNDEFYYPKQQGLRGSFGGNIIQHSIPTVELRQPFFPTHMGPMKLRQFHRPSLKKYSFGALSQPGPHAVQPLLKHIKKKAKMREQERQASGGGDMFFMRTAQDLTGKDGDLVLAEYSEEYPPLHMQVGMASKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYFIRELVDIFVVGQECPLYEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKPDFRLPTEEEIRAMVSPEQCCAYYSMLVAEQRLKDAGYGEKSFFAPEEENEEEFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKVPNKPTQQKDDREPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLESTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLMGEDNERERGRKERRKGSSALSTSSHKDDDASSVTSLNSSATGRRLKIYRTFCDEDGKEYVRCETVRKPAVIDAYLRIRTTKDDEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKDRFKGPPEKKTKKAKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNVRRPHKSIHRRRTDPMVTLSSVLESIINDMRDLPNTYPFHTPVNGKVVKDYYKIITRPMDLQTLRENVRKRMYPSREEFRESVELIFKNSATYNGAKHPLTVVAQAMLSLCVEKIKEKEERLVRLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIINPMDLDTLRKNISKHKYQNREVFLSDVGLIHTNSVKYNGPDSPYTKTALEIVNVCKQTLAEYDEHLTQLEKDILTAKEAALDAADLESLDPLTPGPYTPQPDVCENSVSVSLQGDSSLLAEATLIPPTPEKRGGQGRHRGRLGEEESDVDIEGFEEDDDGKPKTPAPHFQVEDGDLDDEDDDDEDDELLMRPPRRMHIDEDDDDDDEGSSRPAQASVLYQDLLMSDAEDDASEEEGDNPFSSIQLSESGSDSDADLGHQESTRIGLEQEESMMSYEGEGPDGLTHMEDSNVSFGSYDDGDSQMQRQVSSPRTGERDVDEVYGISEEEEDEEEEDERRRGPSVLTQVQLSDDEEDSEEFRSVGGDSDMDSDN

Target ORF information:

RefSeq Version	XM_021476169.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476169.1

ORF Insert Sequence:

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGC CTGATGTGTG TGAGAACAGT 
GTGTCTGTGA GTTTGCAGGG AGACTCTAGT CTGTTAGCCG AGGCTACGCT AATCCCTCCC 
ACCCCTGAGA AAAGAGGGGG GCAGGGTCGA CACAGAGGCA GATTGGGTGA AGAGGAGTCT 
GATGTGGACA TAGAAGGATT TGAGGAAGAT GATGACGGCA AACCGAAAAC ACCTGCTCCG 
CACTTTCAGG TTGAAGATGG AGATCTGGAT GATGAGGATG ACGATGATGA AGATGATGAG 
CTCCTTATGC GGCCACCGAG GCGTATGCAC ATTGATGAAG ATGACGACGA TGATGATGAG 
GGCTCCAGTC GACCAGCACA GGCCAGTGTG CTTTACCAGG ATCTGCTGAT GTCAGATGCT 
GAAGATGATG CCAGTGAGGA GGAAGGCGAT AATCCATTCT CCTCTATCCA ACTGTCTGAG 
AGCGGCAGTG ATAGTGATGC TGACCTGGGA CATCAAGAGA GTACACGGAT TGGACTTGAA 
CAGGAGGAGA GTATGATGTC ATATGAAGGG GAGGGACCTG ACGGCTTAAC GCATATGGAG 
GACAGCAATG TCAGCTTTGG CAGCTATGAT GATGGAGACA GTCAGATGCA GAGGCAGGTG 
TCCAGCCCCA GGACGGGAGA GCGAGATGTA GATGAGGTCT ACGGGATCAG TGAGGAGGAA 
GAGGATGAGG AGGAAGAGGA TGAGCGCAGG AGAGGCCCGA GTGTCCTCAC TCAGGTACAG 
CTCAGTGACG ATGAGGAGGA CAGTGAAGAA TTCAGATCTG TCGGAGGAGA CAGCGACATG 
GACTCTGACA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

taf1 ( XM_021476170.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331845.1 Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X2, mRNA.

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CloneID	ODa142636
Clone ID Related Accession (Same CDS sequence)	XM_021476170.1
Accession Version	XM_021476170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transcription initiation factor TFIID subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATCGGCCTCA TAAGTCTATT 
CACCGCAGGA GGACAGACCC CATGGTCACG CTTTCTTCGG TCCTGGAGAG CATCATTAAT 
GACATGAGGG ATCTTCCAAA TACGTACCCG TTTCACACTC CTGTCAATGG CAAAGTGGTC 
AAGGATTATT ACAAGATCAT CACACGGCCG ATGGATTTGC AGACGTTACG TGAAAACGTT 
CGCAAGCGCA TGTACCCTTC ACGAGAGGAG TTCAGAGAAA GCGTTGAGCT CATCTTCAAA 
AACAGTGCTA CTTATAATGG TGCCAAGCAT CCTTTAACTG TTGTTGCTCA AGCAATGCTG 
AGTCTGTGTG TTGAAAAGAT CAAAGAGAAA GAGGAGCGTC TTGTTCGATT GGAGAAAGCC 
ATTAATCCTC TTCTGGATGA CGATGATCAA GTTGCTTTCT CTTTCATCCT CGACAACATT 
GTCACACAGA AGATGATGGC CGTACCTGAC TCTTGGCCTT TCCATCATCC AGTTAACAAG 
AAATTTGTTC CTGACTATTA TAAAGTCATC ATCAACCCCA TGGATCTGGA CACACTCCGT 
AAGAACATCT CCAAGCACAA GTATCAGAAT CGAGAGGTGT TTCTTTCAGA TGTGGGTCTC 
ATTCACACCA ACAGTGTCAA GTATAATGGT CCTGATAGTC CTTATACAAA AACTGCTCTG 
GAAATTGTGA ACGTTTGCAA GCAGACTTTA GCGGAGTATG ATGAACACCT GACTCAGCTG 
GAGAAAGACA TCCTTACTGC TAAAGAAGCT GCTCTGGACG CTGCAGACCT TGAGAGTTTA 
GATCCATTAA CTCCAGGGCC GTATACACCG CAGCCTGATG TGTGTGAGAA CAGTGTGTCT 
GTGAGTTTGC AGGGAGACTC TAGTCTGTTA GCCGAGGCTA CGCTAATCCC TCCCACCCCT 
GAGAAAAGAG GGGGGCAGGG TCGACACAGA GGCAGATTGG GTGAAGAGGA GTCTGATGTG 
GACATAGAAG GATTTGAGGA AGATGATGAC GGCAAACCGA AAACACCTGC TCCGCACTTT 
CAGGTTGAAG ATGGAGATCT GGATGATGAG GATGACGATG ATGAAGATGA TGAGCTCCTT 
ATGCGGCCAC CGAGGCGTAT GCACATTGAT GAAGATGACG ACGATGATGA TGAGGGCTCC 
AGTCGACCAG CACAGGCCAG TGTGCTTTAC CAGGATCTGC TGATGTCAGA TGCTGAAGAT 
GATGCCAGTG AGGAGGAAGG CGATAATCCA TTCTCCTCTA TCCAACTGTC TGAGAGCGGC 
AGTGATAGTG ATGCTGACCT GGGACATCAA GAGAGTACAC GGATTGGACT TGAACAGGAG 
GAGAGTATGA TGTCATATGA AGGGGAGGGA CCTGACGGCT TAACGCATAT GGAGGACAGC 
AATGTCAGCT TTGGCAGCTA TGATGATGGA GACAGTCAGA TGCAGAGGCA GGTGTCCAGC 
CCCAGGACGG GAGAGCGAGA TGTAGATGAG GTCTACGGGA TCAGTGAGGA GGAAGAGGAT 
GAGGAGGAAG AGGATGAGCG CAGGAGAGGC CCGAGTGTCC TCACTCAGGT ACAGCTCAGT 
GACGATGAGG AGGACAGTGA AGAATTCAGA TCTGTCGGAG GAGACAGCGA CATGGACTCT 
GACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331845.1
CDS	372..6320
Translation	MSDSDSDEDQDRPFHLTGFLFGNINENGQLEDDSVLDTESKKHLAGLGTLGLGSLITEITASEEETAEQEQDQSSTDAEGWVRSTEDAVDYSDISEVAEDETRKYRQAMGNLQPSRRTDDEDDYDADCEDVDSKLMPPPPPPTQPITAKKDDTPTQNSSVTDEGDGIILPSIIAPSSVGDKVDFSSSSDSESESDRPSQGSGTGKKAGCLTLPLAGIMQKDAAKALPGVTELFPEFRPGKVLRFLRLFGPGKNMPSVWRSARRKRKRKQKEPQSDMAAAGDIESLPSEPGAKKKSGWDYEYAPPPPPEQCLSDDEITMMAPVESKFMQVSGEGDKVSEMRPKVAEWRYGPAQLWYDMLGVPEDGSGFHYGFKLKDDKQEDNTDAVTKMATPPASEPVPEQEPPQESQDKADEDAPMQDELFLMVTQLQWEEDIIWNGEDVKHKGTKTQRASLAGWLPTSMTRNANAYNAQQGLSRSNSQLVPPTPPPLTKTPSITGSKRDKHNHDHQVSHEDDTPWFSIFPIDNEELVYGRWEDNIIWDDQNMDCIPSPPVLTLNPNDENIILEIPDEKEERTSHSPSKENKKETALKKSRILLGKTGVIKDEPQQNMSQPEIKDPWNLSNDEFYYPKQQGLRGSFGGNIIQHSIPTVELRQPFFPTHMGPMKLRQFHRPSLKKYSFGALSQPGPHAVQPLLKHIKKKAKMREQERQASGGGDMFFMRTAQDLTGKDGDLVLAEYSEEYPPLHMQVGMASKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYFIRELVDIFVVGQECPLYEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKPDFRLPTEEEIRAMVSPEQCCAYYSMLVAEQRLKDAGYGEKSFFAPEEENEEEFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKVPNKPTQQKDDREPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLESTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLMGEDNERERGRKERRKGSSALSTSSHKDDDASSVTSLNSSATGRRLKIYRTFCDEDGKEYVRCETVRKPAVIDAYLRIRTTKDDEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKDRFKGPPEKKTKKAKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSVLESIINDMRDLPNTYPFHTPVNGKVVKDYYKIITRPMDLQTLRENVRKRMYPSREEFRESVELIFKNSATYNGAKHPLTVVAQAMLSLCVEKIKEKEERLVRLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIINPMDLDTLRKNISKHKYQNREVFLSDVGLIHTNSVKYNGPDSPYTKTALEIVNVCKQTLAEYDEHLTQLEKDILTAKEAALDAADLESLDPLTPGPYTPQPDVCENSVSVSLQGDSSLLAEATLIPPTPEKRGGQGRHRGRLGEEESDVDIEGFEEDDDGKPKTPAPHFQVEDGDLDDEDDDDEDDELLMRPPRRMHIDEDDDDDDEGSSRPAQASVLYQDLLMSDAEDDASEEEGDNPFSSIQLSESGSDSDADLGHQESTRIGLEQEESMMSYEGEGPDGLTHMEDSNVSFGSYDDGDSQMQRQVSSPRTGERDVDEVYGISEEEEDEEEEDERRRGPSVLTQVQLSDDEEDSEEFRSVGGDSDMDSDN

Target ORF information:

RefSeq Version	XM_021476170.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476170.1

ORF Insert Sequence:

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATCGGCCTCA TAAGTCTATT 
CACCGCAGGA GGACAGACCC CATGGTCACG CTTTCTTCGG TCCTGGAGAG CATCATTAAT 
GACATGAGGG ATCTTCCAAA TACGTACCCG TTTCACACTC CTGTCAATGG CAAAGTGGTC 
AAGGATTATT ACAAGATCAT CACACGGCCG ATGGATTTGC AGACGTTACG TGAAAACGTT 
CGCAAGCGCA TGTACCCTTC ACGAGAGGAG TTCAGAGAAA GCGTTGAGCT CATCTTCAAA 
AACAGTGCTA CTTATAATGG TGCCAAGCAT CCTTTAACTG TTGTTGCTCA AGCAATGCTG 
AGTCTGTGTG TTGAAAAGAT CAAAGAGAAA GAGGAGCGTC TTGTTCGATT GGAGAAAGCC 
ATTAATCCTC TTCTGGATGA CGATGATCAA GTTGCTTTCT CTTTCATCCT CGACAACATT 
GTCACACAGA AGATGATGGC CGTACCTGAC TCTTGGCCTT TCCATCATCC AGTTAACAAG 
AAATTTGTTC CTGACTATTA TAAAGTCATC ATCAACCCCA TGGATCTGGA CACACTCCGT 
AAGAACATCT CCAAGCACAA GTATCAGAAT CGAGAGGTGT TTCTTTCAGA TGTGGGTCTC 
ATTCACACCA ACAGTGTCAA GTATAATGGT CCTGATAGTC CTTATACAAA AACTGCTCTG 
GAAATTGTGA ACGTTTGCAA GCAGACTTTA GCGGAGTATG ATGAACACCT GACTCAGCTG 
GAGAAAGACA TCCTTACTGC TAAAGAAGCT GCTCTGGACG CTGCAGACCT TGAGAGTTTA 
GATCCATTAA CTCCAGGGCC GTATACACCG CAGCCTGATG TGTGTGAGAA CAGTGTGTCT 
GTGAGTTTGC AGGGAGACTC TAGTCTGTTA GCCGAGGCTA CGCTAATCCC TCCCACCCCT 
GAGAAAAGAG GGGGGCAGGG TCGACACAGA GGCAGATTGG GTGAAGAGGA GTCTGATGTG 
GACATAGAAG GATTTGAGGA AGATGATGAC GGCAAACCGA AAACACCTGC TCCGCACTTT 
CAGGTTGAAG ATGGAGATCT GGATGATGAG GATGACGATG ATGAAGATGA TGAGCTCCTT 
ATGCGGCCAC CGAGGCGTAT GCACATTGAT GAAGATGACG ACGATGATGA TGAGGGCTCC 
AGTCGACCAG CACAGGCCAG TGTGCTTTAC CAGGATCTGC TGATGTCAGA TGCTGAAGAT 
GATGCCAGTG AGGAGGAAGG CGATAATCCA TTCTCCTCTA TCCAACTGTC TGAGAGCGGC 
AGTGATAGTG ATGCTGACCT GGGACATCAA GAGAGTACAC GGATTGGACT TGAACAGGAG 
GAGAGTATGA TGTCATATGA AGGGGAGGGA CCTGACGGCT TAACGCATAT GGAGGACAGC 
AATGTCAGCT TTGGCAGCTA TGATGATGGA GACAGTCAGA TGCAGAGGCA GGTGTCCAGC 
CCCAGGACGG GAGAGCGAGA TGTAGATGAG GTCTACGGGA TCAGTGAGGA GGAAGAGGAT 
GAGGAGGAAG AGGATGAGCG CAGGAGAGGC CCGAGTGTCC TCACTCAGGT ACAGCTCAGT 
GACGATGAGG AGGACAGTGA AGAATTCAGA TCTGTCGGAG GAGACAGCGA CATGGACTCT 
GACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

taf1 ( XM_021476171.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331846.1 Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X3, mRNA.

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CloneID	ODa142637
Clone ID Related Accession (Same CDS sequence)	XM_021476171.1
Accession Version	XM_021476171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transcription initiation factor TFIID subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGG GAGACTCTAG TCTGTTAGCC 
GAGGCTACGC TAATCCCTCC CACCCCTGAG AAAAGAGGGG GGCAGGGTCG ACACAGAGGC 
AGATTGGGTG AAGAGGAGTC TGATGTGGAC ATAGAAGGAT TTGAGGAAGA TGATGACGGC 
AAACCGAAAA CACCTGCTCC GCACTTTCAG GTTGAAGATG GAGATCTGGA TGATGAGGAT 
GACGATGATG AAGATGATGA GCTCCTTATG CGGCCACCGA GGCGTATGCA CATTGATGAA 
GATGACGACG ATGATGATGA GGGCTCCAGT CGACCAGCAC AGGCCAGTGT GCTTTACCAG 
GATCTGCTGA TGTCAGATGC TGAAGATGAT GCCAGTGAGG AGGAAGGCGA TAATCCATTC 
TCCTCTATCC AACTGTCTGA GAGCGGCAGT GATAGTGATG CTGACCTGGG ACATCAAGAG 
AGTACACGGA TTGGACTTGA ACAGGAGGAG AGTATGATGT CATATGAAGG GGAGGGACCT 
GACGGCTTAA CGCATATGGA GGACAGCAAT GTCAGCTTTG GCAGCTATGA TGATGGAGAC 
AGTCAGATGC AGAGGCAGGT GTCCAGCCCC AGGACGGGAG AGCGAGATGT AGATGAGGTC 
TACGGGATCA GTGAGGAGGA AGAGGATGAG GAGGAAGAGG ATGAGCGCAG GAGAGGCCCG 
AGTGTCCTCA CTCAGGTACA GCTCAGTGAC GATGAGGAGG ACAGTGAAGA ATTCAGATCT 
GTCGGAGGAG ACAGCGACAT GGACTCTGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331846.1
CDS	372..6287
Translation	MSDSDSDEDQDRPFHLTGFLFGNINENGQLEDDSVLDTESKKHLAGLGTLGLGSLITEITASEEETAEQEQDQSSTDAEGWVRSTEDAVDYSDISEVAEDETRKYRQAMGNLQPSRRTDDEDDYDADCEDVDSKLMPPPPPPTQPITAKKDDTPTQNSSVTDEGDGIILPSIIAPSSVGDKVDFSSSSDSESESDRPSQGSGTGKKAGCLTLPLAGIMQKDAAKALPGVTELFPEFRPGKVLRFLRLFGPGKNMPSVWRSARRKRKRKQKEPQSDMAAAGDIESLPSEPGAKKKSGWDYEYAPPPPPEQCLSDDEITMMAPVESKFMQVSGEGDKVSEMRPKVAEWRYGPAQLWYDMLGVPEDGSGFHYGFKLKDDKQEDNTDAVTKMATPPASEPVPEQEPPQESQDKADEDAPMQDELFLMVTQLQWEEDIIWNGEDVKHKGTKTQRASLAGWLPTSMTRNANAYNAQQGLSRSNSQLVPPTPPPLTKTPSITGSKRDKHNHDHQVSHEDDTPWFSIFPIDNEELVYGRWEDNIIWDDQNMDCIPSPPVLTLNPNDENIILEIPDEKEERTSHSPSKENKKETALKKSRILLGKTGVIKDEPQQNMSQPEIKDPWNLSNDEFYYPKQQGLRGSFGGNIIQHSIPTVELRQPFFPTHMGPMKLRQFHRPSLKKYSFGALSQPGPHAVQPLLKHIKKKAKMREQERQASGGGDMFFMRTAQDLTGKDGDLVLAEYSEEYPPLHMQVGMASKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYFIRELVDIFVVGQECPLYEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKPDFRLPTEEEIRAMVSPEQCCAYYSMLVAEQRLKDAGYGEKSFFAPEEENEEEFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKVPNKPTQQKDDREPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLESTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLMGEDNERERGRKERRKGSSALSTSSHKDDDASSVTSLNSSATGRRLKIYRTFCDEDGKEYVRCETVRKPAVIDAYLRIRTTKDDEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKDRFKGPPEKKTKKAKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNVRRPHKSIHRRRTDPMVTLSSVLESIINDMRDLPNTYPFHTPVNGKVVKDYYKIITRPMDLQTLRENVRKRMYPSREEFRESVELIFKNSATYNGAKHPLTVVAQAMLSLCVEKIKEKEERLVRLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIINPMDLDTLRKNISKHKYQNREVFLSDVGLIHTNSVKYNGPDSPYTKTALEIVNVCKQTLAEYDEHLTQLEKDILTAKEAALDAADLESLDPLTPGPYTPQGDSSLLAEATLIPPTPEKRGGQGRHRGRLGEEESDVDIEGFEEDDDGKPKTPAPHFQVEDGDLDDEDDDDEDDELLMRPPRRMHIDEDDDDDDEGSSRPAQASVLYQDLLMSDAEDDASEEEGDNPFSSIQLSESGSDSDADLGHQESTRIGLEQEESMMSYEGEGPDGLTHMEDSNVSFGSYDDGDSQMQRQVSSPRTGERDVDEVYGISEEEEDEEEEDERRRGPSVLTQVQLSDDEEDSEEFRSVGGDSDMDSDN

Target ORF information:

RefSeq Version	XM_021476171.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476171.1

ORF Insert Sequence:

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGG GAGACTCTAG TCTGTTAGCC 
GAGGCTACGC TAATCCCTCC CACCCCTGAG AAAAGAGGGG GGCAGGGTCG ACACAGAGGC 
AGATTGGGTG AAGAGGAGTC TGATGTGGAC ATAGAAGGAT TTGAGGAAGA TGATGACGGC 
AAACCGAAAA CACCTGCTCC GCACTTTCAG GTTGAAGATG GAGATCTGGA TGATGAGGAT 
GACGATGATG AAGATGATGA GCTCCTTATG CGGCCACCGA GGCGTATGCA CATTGATGAA 
GATGACGACG ATGATGATGA GGGCTCCAGT CGACCAGCAC AGGCCAGTGT GCTTTACCAG 
GATCTGCTGA TGTCAGATGC TGAAGATGAT GCCAGTGAGG AGGAAGGCGA TAATCCATTC 
TCCTCTATCC AACTGTCTGA GAGCGGCAGT GATAGTGATG CTGACCTGGG ACATCAAGAG 
AGTACACGGA TTGGACTTGA ACAGGAGGAG AGTATGATGT CATATGAAGG GGAGGGACCT 
GACGGCTTAA CGCATATGGA GGACAGCAAT GTCAGCTTTG GCAGCTATGA TGATGGAGAC 
AGTCAGATGC AGAGGCAGGT GTCCAGCCCC AGGACGGGAG AGCGAGATGT AGATGAGGTC 
TACGGGATCA GTGAGGAGGA AGAGGATGAG GAGGAAGAGG ATGAGCGCAG GAGAGGCCCG 
AGTGTCCTCA CTCAGGTACA GCTCAGTGAC GATGAGGAGG ACAGTGAAGA ATTCAGATCT 
GTCGGAGGAG ACAGCGACAT GGACTCTGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

taf1 ( XM_017356092.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211581.1 Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X2, mRNA.

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CloneID	ODa59083
Clone ID Related Accession (Same CDS sequence)	XM_017356092.1
Accession Version	XM_017356092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transcription initiation factor TFIID subunit 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGG GAGACTCTAG TCTGTTAGCC 
GAGGCTACGC TAATCCCTCC CACCCCTGAG AAAAGAGGGG GGCAGGTATT CCTCTCTCCC 
TCTCCTCATC CATCTGTCCT TCTCTCCATC CATCTCTTCA TTCGTTTTAG TGGCTGGCTT 
GCTTTAGCTC TTTCAATATG TGGTCGCCCC TGTGCTGTGA TGGTTTCTGA TGCCTTCAAA 
CACATCTTCA TTACAGGATT AAACTATGTT CAGGATGTAC TACTCTTTAT TGAGCTTAAA 
CCAAAATCTT TTATAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211581.1
CDS	372..5792
Translation	MSDSDSDEDQDRPFHLTGFLFGNINENGQLEDDSVLDTESKKHLAGLGTLGLGSLITEITASEEETAEQEQDQSSTDAEGWVRSTEDAVDYSDISEVAEDETRKYRQAMGNLQPSRRTDDEDDYDADCEDVDSKLMPPPPPPTQPITAKKDDTPTQNSSVTDEGDGIILPSIIAPSSVGDKVDFSSSSDSESESDRPSQGSGTGKKAGCLTLPLAGIMQKDAAKALPGVTELFPEFRPGKVLRFLRLFGPGKNMPSVWRSARRKRKRKQKEPQSDMAAAGDIESLPSEPGAKKKSGWDYEYAPPPPPEQCLSDDEITMMAPVESKFMQVSGEGDKVSEMRPKVAEWRYGPAQLWYDMLGVPEDGSGFHYGFKLKDDKQEDNTDAVTKMATPPASEPVPEQEPPQESQDKADEDAPMQDELFLMVTQLQWEEDIIWNGEDVKHKGTKTQRASLAGWLPTSMTRNANAYNAQQGLSRSNSQLVPPTPPPLTKTPSITGSKRDKHNHDHQVSHEDDTPWFSIFPIDNEELVYGRWEDNIIWDDQNMDCIPSPPVLTLNPNDENIILEIPDEKEERTSHSPSKENKKETALKKSRILLGKTGVIKDEPQQNMSQPEIKDPWNLSNDEFYYPKQQGLRGSFGGNIIQHSIPTVELRQPFFPTHMGPMKLRQFHRPSLKKYSFGALSQPGPHAVQPLLKHIKKKAKMREQERQASGGGDMFFMRTAQDLTGKDGDLVLAEYSEEYPPLHMQVGMASKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYFIRELVDIFVVGQECPLYEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKPDFRLPTEEEIRAMVSPEQCCAYYSMLVAEQRLKDAGYGEKSFFAPEEENEEEFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKVPNKPTQQKDDREPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLESTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLMGEDNERERGRKERRKGSSALSTSSHKDDDASSVTSLNSSATGRRLKIYRTFCDEDGKEYVRCETVRKPAVIDAYLRIRTTKDDEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKDRFKGPPEKKTKKAKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNVRRPHKSIHRRRTDPMVTLSSVLESIINDMRDLPNTYPFHTPVNGKVVKDYYKIITRPMDLQTLRENVRKRMYPSREEFRESVELIFKNSATYNGAKHPLTVVAQAMLSLCVEKIKEKEERLVRLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIINPMDLDTLRKNISKHKYQNREVFLSDVGLIHTNSVKYNGPDSPYTKTALEIVNVCKQTLAEYDEHLTQLEKDILTAKEAALDAADLESLDPLTPGPYTPQGDSSLLAEATLIPPTPEKRGGQVFLSPSPHPSVLLSIHLFIRFSGWLALALSICGRPCAVMVSDAFKHIFITGLNYVQDVLLFIELKPKSFIK

Target ORF information:

RefSeq Version	XM_017356092.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356092.1

ORF Insert Sequence:

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGG GAGACTCTAG TCTGTTAGCC 
GAGGCTACGC TAATCCCTCC CACCCCTGAG AAAAGAGGGG GGCAGGTATT CCTCTCTCCC 
TCTCCTCATC CATCTGTCCT TCTCTCCATC CATCTCTTCA TTCGTTTTAG TGGCTGGCTT 
GCTTTAGCTC TTTCAATATG TGGTCGCCCC TGTGCTGTGA TGGTTTCTGA TGCCTTCAAA 
CACATCTTCA TTACAGGATT AAACTATGTT CAGGATGTAC TACTCTTTAT TGAGCTTAAA 
CCAAAATCTT TTATAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

taf1 ( NM_001044785.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038250.1 Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), mRNA.

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CloneID	ODa36572
Clone ID Related Accession (Same CDS sequence)	NM_001044785.1
Accession Version	NM_001044785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	transcription initiation factor TFIID subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX005407.5. On Sep 1, 2006 this sequence version replaced XM_677952.1. ##Evidence-Data-START## Transcript exon combination :: GDQH01017237.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATCGGCCTCA TAAGTCTATT 
CACCGCAGGA GGACAGACCC CATGGTCACG CTTTCTTCGG TCCTGGAGAG CATCATTAAT 
GACATGAGGG ATCTTCCAAA TACGTACCCG TTTCACACTC CTGTCAATGG CAAAGTGGTC 
AAGGATTATT ACAAGATCAT CACACGGCCG ATGGATTTGC AGACGTTACG TGAAAACGTT 
CGCAAGCGCA TGTACCCTTC ACGAGAGGAG TTCAGAGAAA GCGTTGAGCT CATCTTCAAA 
AACAGTGCTA CTTATAATGG TGCCAAGCAT CCTTTAACTG TTGTTGCTCA AGCAATGCTG 
AGTCTGTGTG TTGAAAAGAT CAAAGAGAAA GAGGAGCGTC TTGTTCGATT GGAGAAAGCC 
ATTAATCCTC TTCTGGATGA CGATGATCAA GTTGCTTTCT CTTTCATCCT CGACAACATT 
GTCACACAGA AGATGATGGC CGTACCTGAC TCTTGGCCTT TCCATCATCC AGTTAACAAG 
AAATTTGTTC CTGACTATTA TAAAGTCATC ATCAACCCCA TGGATCTGGA CACACTCCGT 
AAGAACATCT CCAAGCACAA GTATCAGAAT CGAGAGGTGT TTCTTTCAGA TGTGGGTCTC 
ATTCACACCA ACAGTGTCAA GTATAATGGT CCTGATAGTC CTTATACAAA AACTGCTCTG 
GAAATTGTGA ACGTTTGCAA GCAGACTTTA GCGGAGTATG ATGAACACCT GACTCAGCTG 
GAGAAAGACA TCCTTACTGC TAAAGAAGCT GCTCTGGACG CTGCAGACCT TGAGAGTTTA 
GATCCATTAA CTCCAGGGCC GTATACACCG CAGGGTCGAC ACAGAGGCAG ATTGGGTGAA 
GAGGAGTCTG ATGTGGACAT AGAAGGATTT GAGGAAGATG ATGACGGCAA ACCGAAAACA 
CCTGCTCCGC ACTTTCAGGT TGAAGATGGA GATCTGGATG ATGAGGATGA CGATGATGAA 
GATGATGAGC TCCTTATGCG GCCACCGAGG CGTATGCACA TTGATGAAGA TGACGACGAT 
GATGATGAGG GCTCCAGTCG ACCAGCACAG GCCAGTGTGC TTTACCAGGA TCTGCTGATG 
TCAGATGCTG AAGATGATGC CAGTGAGGAG GAAGGCGATA ATCCATTCTC CTCTATCCAA 
CTGTCTGAGA GCGGCAGTGA TAGTGATGCT GACCTGGGAC ATCAAGAGAG TACACGGATT 
GGACTTGAAC AGGAGGAGAG TATGATGTCA TATGAAGGGG AGGGACCTGA CGGCTTAACG 
CATATGGAGG ACAGCAATGT CAGCTTTGGC AGCTATGATG ATGGAGACAG TCAGATGCAG 
AGGCAGGTGT CCAGCCCCAG GACGGGAGAG CGAGATGTAG ATGAGGTCTA CGGGATCAGT 
GAGGAGGAAG AGGATGAGGA GGAAGAGGAT GAGCGCAGGA GAGGCCCGAG TGTCCTCACT 
CAGGTACAGC TCAGTGACGA TGAGGAGGAC AGTGAAGAAT TCAGATCTGT CGGAGGAGAC 
AGCGACATGG ACTCTGACAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038250.1
CDS	377..6220
Translation	MSDSDSDEDQDRPFHLTGFLFGNINENGQLEDDSVLDTESKKHLAGLGTLGLGSLITEITASEEETAEQEQDQSSTDAEGWVRSTEDAVDYSDISEVAEDETRKYRQAMGNLQPSRRTDDEDDYDADCEDVDSKLMPPPPPPTQPITAKKDDTPTQNSSVTDEGDGIILPSIIAPSSVGDKVDFSSSSDSESESDRPSQGSGTGKKAGCLTLPLAGIMQKDAAKALPGVTELFPEFRPGKVLRFLRLFGPGKNMPSVWRSARRKRKRKQKEPQSDMAAAGDIESLPSEPGAKKKSGWDYEYAPPPPPEQCLSDDEITMMAPVESKFMQVSGEGDKVSEMRPKVAEWRYGPAQLWYDMLGVPEDGSGFHYGFKLKDDKQEDNTDAVTKMATPPASEPVPEQEPPQESQDKADEDAPMQDELFLMVTQLQWEEDIIWNGEDVKHKGTKTQRASLAGWLPTSMTRNANAYNAQQGLSRSNSQLVPPTPPPLTKTPSITGSKRDKHNHDHQVSHEDDTPWFSIFPIDNEELVYGRWEDNIIWDDQNMDCIPSPPVLTLNPNDENIILEIPDEKEERTSHSPSKENKKETALKKSRILLGKTGVIKDEPQQNMSQPEIKDPWNLSNDEFYYPKQQGLRGSFGGNIIQHSIPTVELRQPFFPTHMGPMKLRQFHRPSLKKYSFGALSQPGPHAVQPLLKHIKKKAKMREQERQASGGGDMFFMRTAQDLTGKDGDLVLAEYSEEYPPLHMQVGMASKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYFIRELVDIFVVGQECPLYEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKPDFRLPTEEEIRAMVSPEQCCAYYSMLVAEQRLKDAGYGEKSFFAPEEENEEEFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKVPNKPTQQKDDREPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLESTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLMGEDNERERGRKERRKGSSALSTSSHKDDDASSVTSLNSSATGRRLKIYRTFCDEDGKEYVRCETVRKPAVIDAYLRIRTTKDDEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKDRFKGPPEKKTKKAKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSVLESIINDMRDLPNTYPFHTPVNGKVVKDYYKIITRPMDLQTLRENVRKRMYPSREEFRESVELIFKNSATYNGAKHPLTVVAQAMLSLCVEKIKEKEERLVRLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIINPMDLDTLRKNISKHKYQNREVFLSDVGLIHTNSVKYNGPDSPYTKTALEIVNVCKQTLAEYDEHLTQLEKDILTAKEAALDAADLESLDPLTPGPYTPQGRHRGRLGEEESDVDIEGFEEDDDGKPKTPAPHFQVEDGDLDDEDDDDEDDELLMRPPRRMHIDEDDDDDDEGSSRPAQASVLYQDLLMSDAEDDASEEEGDNPFSSIQLSESGSDSDADLGHQESTRIGLEQEESMMSYEGEGPDGLTHMEDSNVSFGSYDDGDSQMQRQVSSPRTGERDVDEVYGISEEEEDEEEEDERRRGPSVLTQVQLSDDEEDSEEFRSVGGDSDMDSDN

Target ORF information:

RefSeq Version	NM_001044785.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044785.1

ORF Insert Sequence:

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATCGGCCTCA TAAGTCTATT 
CACCGCAGGA GGACAGACCC CATGGTCACG CTTTCTTCGG TCCTGGAGAG CATCATTAAT 
GACATGAGGG ATCTTCCAAA TACGTACCCG TTTCACACTC CTGTCAATGG CAAAGTGGTC 
AAGGATTATT ACAAGATCAT CACACGGCCG ATGGATTTGC AGACGTTACG TGAAAACGTT 
CGCAAGCGCA TGTACCCTTC ACGAGAGGAG TTCAGAGAAA GCGTTGAGCT CATCTTCAAA 
AACAGTGCTA CTTATAATGG TGCCAAGCAT CCTTTAACTG TTGTTGCTCA AGCAATGCTG 
AGTCTGTGTG TTGAAAAGAT CAAAGAGAAA GAGGAGCGTC TTGTTCGATT GGAGAAAGCC 
ATTAATCCTC TTCTGGATGA CGATGATCAA GTTGCTTTCT CTTTCATCCT CGACAACATT 
GTCACACAGA AGATGATGGC CGTACCTGAC TCTTGGCCTT TCCATCATCC AGTTAACAAG 
AAATTTGTTC CTGACTATTA TAAAGTCATC ATCAACCCCA TGGATCTGGA CACACTCCGT 
AAGAACATCT CCAAGCACAA GTATCAGAAT CGAGAGGTGT TTCTTTCAGA TGTGGGTCTC 
ATTCACACCA ACAGTGTCAA GTATAATGGT CCTGATAGTC CTTATACAAA AACTGCTCTG 
GAAATTGTGA ACGTTTGCAA GCAGACTTTA GCGGAGTATG ATGAACACCT GACTCAGCTG 
GAGAAAGACA TCCTTACTGC TAAAGAAGCT GCTCTGGACG CTGCAGACCT TGAGAGTTTA 
GATCCATTAA CTCCAGGGCC GTATACACCG CAGGGTCGAC ACAGAGGCAG ATTGGGTGAA 
GAGGAGTCTG ATGTGGACAT AGAAGGATTT GAGGAAGATG ATGACGGCAA ACCGAAAACA 
CCTGCTCCGC ACTTTCAGGT TGAAGATGGA GATCTGGATG ATGAGGATGA CGATGATGAA 
GATGATGAGC TCCTTATGCG GCCACCGAGG CGTATGCACA TTGATGAAGA TGACGACGAT 
GATGATGAGG GCTCCAGTCG ACCAGCACAG GCCAGTGTGC TTTACCAGGA TCTGCTGATG 
TCAGATGCTG AAGATGATGC CAGTGAGGAG GAAGGCGATA ATCCATTCTC CTCTATCCAA 
CTGTCTGAGA GCGGCAGTGA TAGTGATGCT GACCTGGGAC ATCAAGAGAG TACACGGATT 
GGACTTGAAC AGGAGGAGAG TATGATGTCA TATGAAGGGG AGGGACCTGA CGGCTTAACG 
CATATGGAGG ACAGCAATGT CAGCTTTGGC AGCTATGATG ATGGAGACAG TCAGATGCAG 
AGGCAGGTGT CCAGCCCCAG GACGGGAGAG CGAGATGTAG ATGAGGTCTA CGGGATCAGT 
GAGGAGGAAG AGGATGAGGA GGAAGAGGAT GAGCGCAGGA GAGGCCCGAG TGTCCTCACT 
CAGGTACAGC TCAGTGACGA TGAGGAGGAC AGTGAAGAAT TCAGATCTGT CGGAGGAGAC 
AGCGACATGG ACTCTGACAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

taf1 ( XM_005165316.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165373.1 Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X4, mRNA.

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CloneID	ODa10706
Clone ID Related Accession (Same CDS sequence)	XM_005165316.4 , XM_005165316.3
Accession Version	XM_005165316.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transcription initiation factor TFIID subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165316.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGG GTCGACACAG AGGCAGATTG 
GGTGAAGAGG AGTCTGATGT GGACATAGAA GGATTTGAGG AAGATGATGA CGGCAAACCG 
AAAACACCTG CTCCGCACTT TCAGGTTGAA GATGGAGATC TGGATGATGA GGATGACGAT 
GATGAAGATG ATGAGCTCCT TATGCGGCCA CCGAGGCGTA TGCACATTGA TGAAGATGAC 
GACGATGATG ATGAGGGCTC CAGTCGACCA GCACAGGCCA GTGTGCTTTA CCAGGATCTG 
CTGATGTCAG ATGCTGAAGA TGATGCCAGT GAGGAGGAAG GCGATAATCC ATTCTCCTCT 
ATCCAACTGT CTGAGAGCGG CAGTGATAGT GATGCTGACC TGGGACATCA AGAGAGTACA 
CGGATTGGAC TTGAACAGGA GGAGAGTATG ATGTCATATG AAGGGGAGGG ACCTGACGGC 
TTAACGCATA TGGAGGACAG CAATGTCAGC TTTGGCAGCT ATGATGATGG AGACAGTCAG 
ATGCAGAGGC AGGTGTCCAG CCCCAGGACG GGAGAGCGAG ATGTAGATGA GGTCTACGGG 
ATCAGTGAGG AGGAAGAGGA TGAGGAGGAA GAGGATGAGC GCAGGAGAGG CCCGAGTGTC 
CTCACTCAGG TACAGCTCAG TGACGATGAG GAGGACAGTG AAGAATTCAG ATCTGTCGGA 
GGAGACAGCG ACATGGACTC TGACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165373.1
CDS	372..6221
Translation	MSDSDSDEDQDRPFHLTGFLFGNINENGQLEDDSVLDTESKKHLAGLGTLGLGSLITEITASEEETAEQEQDQSSTDAEGWVRSTEDAVDYSDISEVAEDETRKYRQAMGNLQPSRRTDDEDDYDADCEDVDSKLMPPPPPPTQPITAKKDDTPTQNSSVTDEGDGIILPSIIAPSSVGDKVDFSSSSDSESESDRPSQGSGTGKKAGCLTLPLAGIMQKDAAKALPGVTELFPEFRPGKVLRFLRLFGPGKNMPSVWRSARRKRKRKQKEPQSDMAAAGDIESLPSEPGAKKKSGWDYEYAPPPPPEQCLSDDEITMMAPVESKFMQVSGEGDKVSEMRPKVAEWRYGPAQLWYDMLGVPEDGSGFHYGFKLKDDKQEDNTDAVTKMATPPASEPVPEQEPPQESQDKADEDAPMQDELFLMVTQLQWEEDIIWNGEDVKHKGTKTQRASLAGWLPTSMTRNANAYNAQQGLSRSNSQLVPPTPPPLTKTPSITGSKRDKHNHDHQVSHEDDTPWFSIFPIDNEELVYGRWEDNIIWDDQNMDCIPSPPVLTLNPNDENIILEIPDEKEERTSHSPSKENKKETALKKSRILLGKTGVIKDEPQQNMSQPEIKDPWNLSNDEFYYPKQQGLRGSFGGNIIQHSIPTVELRQPFFPTHMGPMKLRQFHRPSLKKYSFGALSQPGPHAVQPLLKHIKKKAKMREQERQASGGGDMFFMRTAQDLTGKDGDLVLAEYSEEYPPLHMQVGMASKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYFIRELVDIFVVGQECPLYEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKPDFRLPTEEEIRAMVSPEQCCAYYSMLVAEQRLKDAGYGEKSFFAPEEENEEEFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKVPNKPTQQKDDREPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLESTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLMGEDNERERGRKERRKGSSALSTSSHKDDDASSVTSLNSSATGRRLKIYRTFCDEDGKEYVRCETVRKPAVIDAYLRIRTTKDDEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKDRFKGPPEKKTKKAKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNVRRPHKSIHRRRTDPMVTLSSVLESIINDMRDLPNTYPFHTPVNGKVVKDYYKIITRPMDLQTLRENVRKRMYPSREEFRESVELIFKNSATYNGAKHPLTVVAQAMLSLCVEKIKEKEERLVRLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIINPMDLDTLRKNISKHKYQNREVFLSDVGLIHTNSVKYNGPDSPYTKTALEIVNVCKQTLAEYDEHLTQLEKDILTAKEAALDAADLESLDPLTPGPYTPQGRHRGRLGEEESDVDIEGFEEDDDGKPKTPAPHFQVEDGDLDDEDDDDEDDELLMRPPRRMHIDEDDDDDDEGSSRPAQASVLYQDLLMSDAEDDASEEEGDNPFSSIQLSESGSDSDADLGHQESTRIGLEQEESMMSYEGEGPDGLTHMEDSNVSFGSYDDGDSQMQRQVSSPRTGERDVDEVYGISEEEEDEEEEDERRRGPSVLTQVQLSDDEEDSEEFRSVGGDSDMDSDN

Target ORF information:

RefSeq Version	XM_005165316.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165316.4

ORF Insert Sequence:

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGG GTCGACACAG AGGCAGATTG 
GGTGAAGAGG AGTCTGATGT GGACATAGAA GGATTTGAGG AAGATGATGA CGGCAAACCG 
AAAACACCTG CTCCGCACTT TCAGGTTGAA GATGGAGATC TGGATGATGA GGATGACGAT 
GATGAAGATG ATGAGCTCCT TATGCGGCCA CCGAGGCGTA TGCACATTGA TGAAGATGAC 
GACGATGATG ATGAGGGCTC CAGTCGACCA GCACAGGCCA GTGTGCTTTA CCAGGATCTG 
CTGATGTCAG ATGCTGAAGA TGATGCCAGT GAGGAGGAAG GCGATAATCC ATTCTCCTCT 
ATCCAACTGT CTGAGAGCGG CAGTGATAGT GATGCTGACC TGGGACATCA AGAGAGTACA 
CGGATTGGAC TTGAACAGGA GGAGAGTATG ATGTCATATG AAGGGGAGGG ACCTGACGGC 
TTAACGCATA TGGAGGACAG CAATGTCAGC TTTGGCAGCT ATGATGATGG AGACAGTCAG 
ATGCAGAGGC AGGTGTCCAG CCCCAGGACG GGAGAGCGAG ATGTAGATGA GGTCTACGGG 
ATCAGTGAGG AGGAAGAGGA TGAGGAGGAA GAGGATGAGC GCAGGAGAGG CCCGAGTGTC 
CTCACTCAGG TACAGCTCAG TGACGATGAG GAGGACAGTG AAGAATTCAG ATCTGTCGGA 
GGAGACAGCG ACATGGACTC TGACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

taf1 ( XM_005165316.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165373.1 Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X1, mRNA.

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CloneID	ODa10706
Clone ID Related Accession (Same CDS sequence)	XM_005165316.4 , XM_005165316.3
Accession Version	XM_005165316.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transcription initiation factor TFIID subunit 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGG GTCGACACAG AGGCAGATTG 
GGTGAAGAGG AGTCTGATGT GGACATAGAA GGATTTGAGG AAGATGATGA CGGCAAACCG 
AAAACACCTG CTCCGCACTT TCAGGTTGAA GATGGAGATC TGGATGATGA GGATGACGAT 
GATGAAGATG ATGAGCTCCT TATGCGGCCA CCGAGGCGTA TGCACATTGA TGAAGATGAC 
GACGATGATG ATGAGGGCTC CAGTCGACCA GCACAGGCCA GTGTGCTTTA CCAGGATCTG 
CTGATGTCAG ATGCTGAAGA TGATGCCAGT GAGGAGGAAG GCGATAATCC ATTCTCCTCT 
ATCCAACTGT CTGAGAGCGG CAGTGATAGT GATGCTGACC TGGGACATCA AGAGAGTACA 
CGGATTGGAC TTGAACAGGA GGAGAGTATG ATGTCATATG AAGGGGAGGG ACCTGACGGC 
TTAACGCATA TGGAGGACAG CAATGTCAGC TTTGGCAGCT ATGATGATGG AGACAGTCAG 
ATGCAGAGGC AGGTGTCCAG CCCCAGGACG GGAGAGCGAG ATGTAGATGA GGTCTACGGG 
ATCAGTGAGG AGGAAGAGGA TGAGGAGGAA GAGGATGAGC GCAGGAGAGG CCCGAGTGTC 
CTCACTCAGG TACAGCTCAG TGACGATGAG GAGGACAGTG AAGAATTCAG ATCTGTCGGA 
GGAGACAGCG ACATGGACTC TGACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165373.1
CDS	372..6221
Translation	MSDSDSDEDQDRPFHLTGFLFGNINENGQLEDDSVLDTESKKHLAGLGTLGLGSLITEITASEEETAEQEQDQSSTDAEGWVRSTEDAVDYSDISEVAEDETRKYRQAMGNLQPSRRTDDEDDYDADCEDVDSKLMPPPPPPTQPITAKKDDTPTQNSSVTDEGDGIILPSIIAPSSVGDKVDFSSSSDSESESDRPSQGSGTGKKAGCLTLPLAGIMQKDAAKALPGVTELFPEFRPGKVLRFLRLFGPGKNMPSVWRSARRKRKRKQKEPQSDMAAAGDIESLPSEPGAKKKSGWDYEYAPPPPPEQCLSDDEITMMAPVESKFMQVSGEGDKVSEMRPKVAEWRYGPAQLWYDMLGVPEDGSGFHYGFKLKDDKQEDNTDAVTKMATPPASEPVPEQEPPQESQDKADEDAPMQDELFLMVTQLQWEEDIIWNGEDVKHKGTKTQRASLAGWLPTSMTRNANAYNAQQGLSRSNSQLVPPTPPPLTKTPSITGSKRDKHNHDHQVSHEDDTPWFSIFPIDNEELVYGRWEDNIIWDDQNMDCIPSPPVLTLNPNDENIILEIPDEKEERTSHSPSKENKKETALKKSRILLGKTGVIKDEPQQNMSQPEIKDPWNLSNDEFYYPKQQGLRGSFGGNIIQHSIPTVELRQPFFPTHMGPMKLRQFHRPSLKKYSFGALSQPGPHAVQPLLKHIKKKAKMREQERQASGGGDMFFMRTAQDLTGKDGDLVLAEYSEEYPPLHMQVGMASKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYFIRELVDIFVVGQECPLYEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKPDFRLPTEEEIRAMVSPEQCCAYYSMLVAEQRLKDAGYGEKSFFAPEEENEEEFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKVPNKPTQQKDDREPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLESTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLMGEDNERERGRKERRKGSSALSTSSHKDDDASSVTSLNSSATGRRLKIYRTFCDEDGKEYVRCETVRKPAVIDAYLRIRTTKDDEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKDRFKGPPEKKTKKAKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNVRRPHKSIHRRRTDPMVTLSSVLESIINDMRDLPNTYPFHTPVNGKVVKDYYKIITRPMDLQTLRENVRKRMYPSREEFRESVELIFKNSATYNGAKHPLTVVAQAMLSLCVEKIKEKEERLVRLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIINPMDLDTLRKNISKHKYQNREVFLSDVGLIHTNSVKYNGPDSPYTKTALEIVNVCKQTLAEYDEHLTQLEKDILTAKEAALDAADLESLDPLTPGPYTPQGRHRGRLGEEESDVDIEGFEEDDDGKPKTPAPHFQVEDGDLDDEDDDDEDDELLMRPPRRMHIDEDDDDDDEGSSRPAQASVLYQDLLMSDAEDDASEEEGDNPFSSIQLSESGSDSDADLGHQESTRIGLEQEESMMSYEGEGPDGLTHMEDSNVSFGSYDDGDSQMQRQVSSPRTGERDVDEVYGISEEEEDEEEEDERRRGPSVLTQVQLSDDEEDSEEFRSVGGDSDMDSDN

Target ORF information:

RefSeq Version	XM_005165316.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (taf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165316.3

ORF Insert Sequence:

ATGTCAGACT CGGACAGTGA TGAGGATCAG GATCGGCCCT TCCACTTGAC AGGGTTTCTT 
TTTGGCAACA TTAACGAGAA TGGACAGCTG GAAGATGACA GTGTGCTGGA TACGGAGTCA 
AAGAAGCATT TGGCTGGTCT GGGAACGCTG GGTTTGGGTT CACTCATTAC AGAGATCACA 
GCCAGCGAGG AGGAAACAGC CGAGCAAGAG CAAGATCAGA GCAGCACTGA CGCAGAAGGA 
TGGGTGAGGA GCACTGAAGA TGCAGTTGAT TACTCTGATA TCAGTGAAGT TGCCGAGGAT 
GAAACTCGCA AATACAGACA GGCCATGGGC AACCTGCAGC CCTCGCGGAG AACAGATGAC 
GAGGATGACT ATGATGCTGA TTGTGAGGAT GTTGATTCCA AACTCATGCC ACCTCCCCCT 
CCCCCTACTC AACCAATCAC GGCGAAGAAA GATGACACGC CCACTCAGAA CAGCAGTGTG 
ACTGATGAGG GAGATGGTAT CATCCTGCCT TCCATTATTG CTCCGTCATC TGTGGGTGAT 
AAGGTTGATT TCAGCAGTTC ATCGGACAGC GAGAGTGAGA GCGATCGACC TTCTCAGGGT 
TCAGGGACAG GGAAAAAGGC AGGGTGTCTC ACCCTCCCTC TCGCAGGGAT TATGCAAAAA 
GATGCTGCCA AAGCCCTGCC TGGAGTCACT GAGCTCTTTC CTGAGTTCAG ACCTGGAAAG 
GTGTTGCGGT TCTTGCGGTT GTTTGGTCCT GGAAAGAACA TGCCATCTGT ATGGCGAAGT 
GCGCGCAGGA AGCGAAAACG AAAGCAGAAA GAGCCACAGT CTGACATGGC AGCTGCGGGT 
GACATTGAAT CACTGCCATC TGAACCAGGG GCTAAGAAAA AATCAGGGTG GGACTATGAG 
TATGCACCGC CTCCACCCCC AGAGCAGTGT CTGTCTGATG ATGAGATCAC CATGATGGCT 
CCGGTGGAGT CTAAGTTCAT GCAGGTGTCT GGTGAAGGTG ATAAAGTGTC TGAGATGAGG 
CCAAAGGTGG CTGAGTGGCG TTATGGTCCA GCACAGCTTT GGTATGACAT GCTTGGTGTC 
CCGGAGGATG GCAGCGGCTT TCATTATGGA TTTAAGCTGA AAGATGACAA ACAGGAAGAC 
AACACTGATG CAGTGACAAA AATGGCAACT CCTCCAGCTT CTGAACCAGT GCCTGAGCAG 
GAACCACCAC AAGAATCACA AGATAAGGCG GATGAGGATG CTCCAATGCA GGATGAGCTG 
TTTTTAATGG TGACCCAACT GCAGTGGGAG GAAGACATTA TCTGGAATGG AGAGGATGTA 
AAACACAAGG GCACTAAAAC TCAGCGTGCT AGTCTGGCTG GGTGGCTGCC TACTAGCATG 
ACCCGAAACG CCAATGCTTA CAACGCTCAG CAGGGTCTAA GCCGCAGTAA TTCTCAGTTG 
GTGCCCCCAA CCCCTCCTCC TTTGACCAAA ACTCCATCCA TAACAGGATC CAAGAGAGAC 
AAACATAATC ATGATCATCA GGTTTCTCAT GAGGATGACA CCCCATGGTT CTCAATATTC 
CCAATTGATA ACGAGGAGCT TGTTTATGGA CGTTGGGAAG ACAACATCAT TTGGGATGAT 
CAGAACATGG ACTGTATCCC TTCACCTCCT GTTCTCACCC TTAACCCCAA TGATGAGAAC 
ATCATACTAG AGATCCCAGA TGAAAAAGAG GAGAGAACCT CACATTCACC CTCCAAAGAA 
AATAAAAAAG AGACAGCATT GAAAAAAAGT CGCATTCTGC TGGGAAAGAC TGGCGTCATC 
AAAGATGAAC CCCAACAGAA TATGTCCCAA CCGGAGATCA AAGATCCCTG GAATCTGTCC 
AATGATGAGT TCTACTACCC CAAACAGCAG GGCCTCAGGG GCTCATTTGG CGGAAACATC 
ATTCAGCACT CCATTCCTAC GGTTGAGTTG AGGCAGCCGT TCTTCCCCAC TCACATGGGA 
CCCATGAAAC TACGACAGTT CCATCGACCT TCTCTGAAGA AGTACTCATT TGGAGCACTG 
TCCCAGCCTG GTCCGCACGC AGTCCAACCA CTTCTCAAAC ACATCAAGAA AAAGGCTAAG 
ATGCGAGAAC AGGAGCGGCA GGCATCAGGT GGAGGGGACA TGTTCTTTAT GCGCACTGCT 
CAGGATCTGA CAGGCAAAGA CGGAGACCTG GTTTTGGCTG AATACAGTGA GGAGTATCCT 
CCTCTTCACA TGCAAGTTGG CATGGCATCT AAGATTAAGA ACTATTACAA AAGAAAACCA 
GGTAAAGACC CAGGCGCACC TGACTGTAAA TATGGAGAGA CCGTTTACTG CCACACTTCA 
CCATTCCTTG GATCATTGCA CCCTGGACAG CTGCTACAGG CATTTGAAAA TAACTTGTTT 
AGAGCTCCCA TATACCTTCA TAAGATGCCT GAGACAGATT TCCTCATTAT CCGGACGAGG 
CAGGGTTACT TTATCCGGGA GCTTGTGGAC ATATTTGTTG TGGGTCAGGA GTGCCCTCTT 
TATGAGGTGC CAGGGCCCAA CTCTAAACGA GCCAACACAC ACATTCGAGA TTTTCTTCAG 
GTTTTCATCT ATCGTCTGTT CTGGAAGAGT AAGGATCGTC CTCGCCGCAT TCGTATGGAA 
GACATAAAGA AAGCGTTTCC ATCTCACTCT GAAAGCAGCA TCCGCAAACG CCTCAAACTC 
TGTGCTGACT TTAAACGCAC AGGCATGGAC TCTAACTGGT GGGTGCTGAA ACCTGATTTC 
CGGCTTCCGA CTGAAGAGGA GATCAGAGCG ATGGTCTCCC CAGAGCAGTG TTGTGCATAC 
TACAGTATGC TGGTGGCAGA GCAGAGGCTG AAGGATGCTG GTTATGGTGA GAAGTCTTTC 
TTTGCACCCG AGGAAGAGAA TGAGGAGGAA TTCCAGATGA AGATTGACGA TGAGGTACGC 
ACTGCACCAT GGAACACAAC TCGGGCATTT ATTGCAGCCA TGAAGGGCAA GTGTCTCCTG 
GAGGTCACAG GGGTTGCTGA CCCTACCGGG TGTGGAGAGG GATTCTCATA TGTTAAAGTC 
CCTAACAAAC CTACGCAGCA GAAGGATGAT CGAGAGCCTC AGCCAGTAAA AAAGACAGTA 
ACTGGGACTG ATGCTGATCT GAGACGCCTA TCACTCAAAA ATGCCAAGCA GTTGCTTCGC 
AAGTTTGGGG TTCCTGAAGA AGAGATAAAG AAACTGTCCC GTTGGGAGGT TATCGATGTC 
GTGAGGACCA TGTCTACTGA GCAGGCTCGT TCAGGGGAAG GACCAATGAG TAAATTTGCT 
CGTGGGTCTC GATTCTCTGT AGCCGAACAT CAAGAACGTT ACAAAGAGGA GTGCCAGAGG 
ATCTTTGACC TGCAGAACAA AGTACTAGAG TCCACAGAGG TGTTATCGAC AGACACAGAC 
AGCAGCTCAG CGGAGGACAG TGATTTTGAG GAGATGGGCA AGAACATCGA GAACATGCTG 
CAAAATAAGA AGACCAGCTC TCAGCTGAGC AGAGAGAGAG AAGAGCAGGA GAGGAAAGAG 
CTGCAGCGAA TGCTGATGGG AGAGGACAAC GAGAGGGAGA GGGGCCGCAA GGAGAGACGC 
AAAGGCTCGA GTGCCCTCTC CACCAGCTCT CACAAAGATG ATGACGCTTC CTCCGTCACT 
AGTCTGAACT CCTCAGCAAC AGGGCGAAGG CTGAAAATCT ATCGCACCTT TTGTGATGAG 
GATGGCAAGG AGTATGTGCG CTGCGAGACT GTACGCAAAC CGGCTGTTAT TGACGCTTAT 
CTGCGTATAC GCACTACTAA GGATGATGAG TTCATTCGCA AGTTTGCTTT GTTTGATGAG 
CAGCACAGGG AGGAGATGAG AAAGGAACGG CGCAGAATTC AGGAGCAGCT CCGACGCCTC 
AAACGAAACC AAGAGAAAGA CCGATTTAAA GGACCACCTG AGAAAAAGAC CAAGAAGGCT 
AAAGAACGAC CAGACCTGAA GCTGAAATGT GGAGCTTGTG GTGCAATTGG CCATATGAGA 
ACCAACAAGT TTTGCCCGCT GTACTACCAA ACCAATGCTC CCCCCTCCAA TCCGGTGGCT 
ATGACAGAGG AACAGGAAGA GGAGCTTGAG AAAACTGTAA TCCACAATGA TAATGAAGAA 
CTCATTAAAG TAGAAGGAAC CAAGATTGTG CTTGGAAAAC AACTCATTGA GAGTGCTGAT 
GAAGTGCGGA GGAAATCTCT GGTTCTGAAG TTCCCCAAGC AGCAGCTTCC TCCTAAAAAG 
AAGAGGCGTG TGGGGACAAC GGTGCACTGT GACTACCTCA ATGTAAGACG GCCTCATAAG 
TCTATTCACC GCAGGAGGAC AGACCCCATG GTCACGCTTT CTTCGGTCCT GGAGAGCATC 
ATTAATGACA TGAGGGATCT TCCAAATACG TACCCGTTTC ACACTCCTGT CAATGGCAAA 
GTGGTCAAGG ATTATTACAA GATCATCACA CGGCCGATGG ATTTGCAGAC GTTACGTGAA 
AACGTTCGCA AGCGCATGTA CCCTTCACGA GAGGAGTTCA GAGAAAGCGT TGAGCTCATC 
TTCAAAAACA GTGCTACTTA TAATGGTGCC AAGCATCCTT TAACTGTTGT TGCTCAAGCA 
ATGCTGAGTC TGTGTGTTGA AAAGATCAAA GAGAAAGAGG AGCGTCTTGT TCGATTGGAG 
AAAGCCATTA ATCCTCTTCT GGATGACGAT GATCAAGTTG CTTTCTCTTT CATCCTCGAC 
AACATTGTCA CACAGAAGAT GATGGCCGTA CCTGACTCTT GGCCTTTCCA TCATCCAGTT 
AACAAGAAAT TTGTTCCTGA CTATTATAAA GTCATCATCA ACCCCATGGA TCTGGACACA 
CTCCGTAAGA ACATCTCCAA GCACAAGTAT CAGAATCGAG AGGTGTTTCT TTCAGATGTG 
GGTCTCATTC ACACCAACAG TGTCAAGTAT AATGGTCCTG ATAGTCCTTA TACAAAAACT 
GCTCTGGAAA TTGTGAACGT TTGCAAGCAG ACTTTAGCGG AGTATGATGA ACACCTGACT 
CAGCTGGAGA AAGACATCCT TACTGCTAAA GAAGCTGCTC TGGACGCTGC AGACCTTGAG 
AGTTTAGATC CATTAACTCC AGGGCCGTAT ACACCGCAGG GTCGACACAG AGGCAGATTG 
GGTGAAGAGG AGTCTGATGT GGACATAGAA GGATTTGAGG AAGATGATGA CGGCAAACCG 
AAAACACCTG CTCCGCACTT TCAGGTTGAA GATGGAGATC TGGATGATGA GGATGACGAT 
GATGAAGATG ATGAGCTCCT TATGCGGCCA CCGAGGCGTA TGCACATTGA TGAAGATGAC 
GACGATGATG ATGAGGGCTC CAGTCGACCA GCACAGGCCA GTGTGCTTTA CCAGGATCTG 
CTGATGTCAG ATGCTGAAGA TGATGCCAGT GAGGAGGAAG GCGATAATCC ATTCTCCTCT 
ATCCAACTGT CTGAGAGCGG CAGTGATAGT GATGCTGACC TGGGACATCA AGAGAGTACA 
CGGATTGGAC TTGAACAGGA GGAGAGTATG ATGTCATATG AAGGGGAGGG ACCTGACGGC 
TTAACGCATA TGGAGGACAG CAATGTCAGC TTTGGCAGCT ATGATGATGG AGACAGTCAG 
ATGCAGAGGC AGGTGTCCAG CCCCAGGACG GGAGAGCGAG ATGTAGATGA GGTCTACGGG 
ATCAGTGAGG AGGAAGAGGA TGAGGAGGAA GAGGATGAGC GCAGGAGAGG CCCGAGTGTC 
CTCACTCAGG TACAGCTCAG TGACGATGAG GAGGACAGTG AAGAATTCAG ATCTGTCGGA 
GGAGACAGCG ACATGGACTC TGACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZNHIT3 is defective in PEHO syndrome, a severe encephalopathy with cerebellar granule neuron loss.
Brain : a journal of neurology140(5)1267-1279(2017 05)
Anttonen AK,Laari A,Kousi M,Yang YJ,J??skel?inen T,Somer M,Siintola E,Jakkula E,Muona M,Tegelberg S,L?nnqvist T,Pihko H,Valanne L,Paetau A,Lun MP,H?stbacka J,Kopra O,Joensuu T,Katsanis N,Lehtinen MK,Palvimo JJ,Lehesjoki AE

TAF1 Variants Are Associated with Dysmorphic Features, Intellectual Disability, and Neurological Manifestations.
American journal of human genetics97(6)922-32(2015 Dec)
O'Rawe JA,Wu Y,D?rfel MJ,Rope AF,Au PY,Parboosingh JS,Moon S,Kousi M,Kosma K,Smith CS,Tzetis M,Schuette JL,Hufnagel RB,Prada CE,Martinez F,Orellana C,Crain J,Caro-Llopis A,Oltra S,Monfort S,Jim?nez-Barr?n LT,Swensen J,Ellingwood S,Smith R,Fang H,Ospina S,Stegmann S,Den Hollander N,Mittelman D,Highnam G,Robison R,Yang E,Faivre L,Roubertie A,Rivi?re JB,Monaghan KG,Wang K,Davis EE,Katsanis N,Kalscheuer VM,Wang EH,Metcalfe K,Kleefstra T,Innes AM,Kitsiou-Tzeli S,Rosello M,Keegan CE,Lyon GJ

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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taf1 cDNA ORF clone, Danio rerio(zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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