lnx2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lnx2a
Entrez Gene ID	553331
Full Name	ligand of numb-protein X 2a
Synonyms	lnx2
General protein information	Preferred Names ligand of numb-protein X 2a Names ligand of Numb protein X 2 ligand of numb-protein X 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470169.1	XP_021325844.1	ligand of Numb protein X 2 isoform X1
XM_021470171.1	XP_021325846.1	ligand of Numb protein X 2 isoform X3
XM_021470170.1	XP_021325845.1	ligand of Numb protein X 2 isoform X2
NM_001113225.2	NP_001106696.2	ligand of Numb protein X 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LNX2	XP_522642.1
Canis lupus familiaris (dog)	LNX2	XP_005635567.1
Bos taurus (cattle)	LNX2	NP_001069352.1
Mus musculus (house mouse)	Lnx2	NP_542985.4
	LNX2	XP_001096316.1
Gallus gallus (chicken)	LNX2	XP_417122.2
Danio rerio (zebrafish)	lnx2a	NP_001106696.2
Xenopus tropicalis (tropical clawed frog)	lnx2	NP_001072606.1
Rattus norvegicus (Norway rat)	Lnx2	NP_001101799.1
Homo sapiens (human)	LNX2	NP_699202.1

Gene Ontology
Bibliography

Related articles in PubMed

PCR artifact in testing for homologous recombination in genomic editing in zebrafish.
Won M, Dawid IB
PloS one12(3)e0172802(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas.
Won M, Ro H, Dawid IB
Proceedings of the National Academy of Sciences of the United States of America112(40)12426-31(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the phenotype characterized by inhibition of exocrine cell differentiation requires inactivation of both ubiquitin ligases Lnx2a and Lnx2b.
Title: Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas.

The following lnx2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lnx2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00399	NM_001113225.2 Latest version!	Danio rerio ligand of numb-protein X 2a (lnx2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa151691	XM_021470169.1 Latest version!	Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa151692	XM_021470170.1 Latest version!	Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa151693	XM_021470171.1 Latest version!	Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lnx2a ( NM_001113225.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001106696.2 Danio rerio ligand of numb-protein X 2a (lnx2a), mRNA.

Price & Availability↑

CloneID	ODa00399
Clone ID Related Accession (Same CDS sequence)	NM_001113225.2
Accession Version	NM_001113225.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-27
Organism	Danio rerio(zebrafish)
Product	ligand of Numb protein X 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FJ156085.1, EB950577.1, EH583415.1, BX571696.7, EB957408.1, DT867995.1 and BG305543.1. On May 1, 2009 this sequence version replaced NM_001113225.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FJ156085.1, GDQH01027022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476820 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGTC TGGGAGAGGG AGTGGACCCG GGCATGGTGA GCGAAGCTCT TCTGGAGGCC 
CTTTGCTCTG AATGTGGACA GATTCACCGC ACCTGGGAGA ACCACCTTTA TAACTATCGT 
TTGGAAGTGG ATGATGATTT GGTGTGCCAC ATCTGCCTTC AGCCGTTAGT TCAGCCCCTG 
GATACACCAT GTGGCCACAC CTTCTGTGCC CGCTGTCTGC GCAGCTTCCT GCAGGAACGG 
GACTTCTGTC CACTGGACCG TGCGCATCTG CAGCTGCAGG TCTGCCGGCG CTCCAGCATT 
CTGGTGCACA AGCTGCTGGA CAAGTTGTCG GTCACCTGTC CGCTCACGCC CTCCTGCTCG 
CTCAGCATGC CTCGCTGCGA CCTGGAGGCA CATCTCAAAC ACAGGTGTCC TGGAACACAG 
AGTCAGCGAA CAAAGCTAGA GCGGACGCAG ATGGAGGGAA GTGAGGACAG GGTCACAGCG 
ACCAATCCCC CCAAATCCCC CCAGACTGAG ATAAAGGAAG ACACGCCATC ACCACCTGCT 
GGATCCAATG CATGCAACAA TGTTCCTACA TGGACAGAAG AGCCTGGTAT AGACAACCCT 
GCCTTTGAAG AAAGCACAGA AGAAGACAGT GTTCATGGTC TTGAGTGTGC TCTTCCACGT 
GTGAAGCGTC CTCTCAGCAA CCCATGCATT CATCTTCTTC GCACTGGCAG TTCGGCTTCT 
TCAGGATGGG ACTTTGTTGA AACTCTTCCT CTTTCTGCTG AAGAAGGTTG TGTAAAACTT 
CCTTCTCTAC CTGAGGGAGA AATCACTACT ATTGAGGTTC ACCGTACAAA CCCCTACTCG 
GAGATGGGCA TAAGTATTGT GGGTGGAAAT GAGACTCCCC TGATAAATGT GGTAATACAG 
GAGGTGTACC GTGACGGTGT TATAGCCCGA GACGGAAGGC TGCTGGCCGG AGACCAAATA 
CTTCAGGTGA ACAATGTAGA CATCAGTAAC GTGCCCCATA ACTTTGCTCG CTCTACACTG 
GCCCGTCCCT GTGCTACCCT TCAGCTGACG GTTTTAAGAG AGCGCCGCTG CTCTGCCAGG 
CCACCTGCAG CAACTGCATC ACCCAAGGGC AGCCCTGCCA GCATACGCAT CACCCTGCAC 
AAGAGGGAAT CATCAGAGCA GCTGGGCATC AAGCTTGTGC GGCGTACAGA TGAAGCCGGC 
GTTTTTATTC TGGATCTGCT GGAGGGAGGC TTAGCTGCGA AAGATGGGCG ACTCTGCAGC 
AACGACAGGG TGCTGGCTGT CAATGAGCAT GACCTGAGAC ATGGCACGCC AGAGCTGGCA 
GCTCAGATTA TACAGGCTAG CGGAGAAAGA GTCAATCTGC TGATTAGCCG GTCCAGCAAG 
CAGACTATGG CGGTGCACAC AGGCTCCACC TTGACCAGGG ACATTTGGAG CCATGATCAC 
ATCCCGCCAC TTCCCAGTAC TGCGACCCCA AGTCCCGTCC CCAGTCTGCA CCTTGCCAGG 
TCTTCCACCC AGAGAGATCT CTCCCAGTGT GTGAACTGTA AAGAGAAACA CATCACTGTG 
AAGAAGGAGC CTCATGAGTC TCTGGGAATG ACTGTGGCAG GAGGACGGGG CAGCAAAAGT 
GGGGAACTGC CCATATTTGT GACGAGCGTC CAGCCCCATG GCTGCCTGTC ACGTGACGGA 
CGAATCAAAC GTGGGGATGT ACTGTTAAGT ATTAACGGTC AGGATTTGAC CTACCTGAGC 
CACAGCGAAG CAGTTGGCAC CCTGAAGTCC AGCGCCACGT CCTGCTCTGT TCAGCTGAAA 
GCACTAGAAG TCACCATGGT GGAAGAGCCA GGCCTGGACG AAGAGCTTCT CCCTCCACAC 
GAAAATGACT ACGATGCAAG CTGGTCGCCA TCATGGGTCT TGTGGCTTGG GCTTCCCAGC 
TACCTGCATA GCAGTCATGA GATCGTCCTC CGCAGGAGTC ACCCTGGCAG CTGGGGCTTC 
AGCATTGTTG GAGGTTATGA GGAGAACCAC AGCAACCAGG CATTCTTTAT CAAGACTATC 
GTCCTTGGAA CTCCCGCATA CTACGATGGT CGTCTAAAGT GTGGTGACAT GATAGTTGCT 
GTAAACGGTC TGTCCACAGC TGGCATGAGT CATTCCGCGC TTGTGCCCAT GCTTAAGGAG 
CAGCGGAGCA GGGTGGCGCT TACGGTTGTG TCCTGGCCAG GAAGTCTGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106696.2
CDS	244..2457
Translation	MGSLGEGVDPGMVSEALLEALCSECGQIHRTWENHLYNYRLEVDDDLVCHICLQPLVQPLDTPCGHTFCARCLRSFLQERDFCPLDRAHLQLQVCRRSSILVHKLLDKLSVTCPLTPSCSLSMPRCDLEAHLKHRCPGTQSQRTKLERTQMEGSEDRVTATNPPKSPQTEIKEDTPSPPAGSNACNNVPTWTEEPGIDNPAFEESTEEDSVHGLECALPRVKRPLSNPCIHLLRTGSSASSGWDFVETLPLSAEEGCVKLPSLPEGEITTIEVHRTNPYSEMGISIVGGNETPLINVVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNVPHNFARSTLARPCATLQLTVLRERRCSARPPAATASPKGSPASIRITLHKRESSEQLGIKLVRRTDEAGVFILDLLEGGLAAKDGRLCSNDRVLAVNEHDLRHGTPELAAQIIQASGERVNLLISRSSKQTMAVHTGSTLTRDIWSHDHIPPLPSTATPSPVPSLHLARSSTQRDLSQCVNCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINGQDLTYLSHSEAVGTLKSSATSCSVQLKALEVTMVEEPGLDEELLPPHENDYDASWSPSWVLWLGLPSYLHSSHEIVLRRSHPGSWGFSIVGGYEENHSNQAFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRVALTVVSWPGSLI

Target ORF information:

RefSeq Version	NM_001113225.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ligand of numb-protein X 2a (lnx2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113225.2

ORF Insert Sequence:

ATGGGCAGTC TGGGAGAGGG AGTGGACCCG GGCATGGTGA GCGAAGCTCT TCTGGAGGCC 
CTTTGCTCTG AATGTGGACA GATTCACCGC ACCTGGGAGA ACCACCTTTA TAACTATCGT 
TTGGAAGTGG ATGATGATTT GGTGTGCCAC ATCTGCCTTC AGCCGTTAGT TCAGCCCCTG 
GATACACCAT GTGGCCACAC CTTCTGTGCC CGCTGTCTGC GCAGCTTCCT GCAGGAACGG 
GACTTCTGTC CACTGGACCG TGCGCATCTG CAGCTGCAGG TCTGCCGGCG CTCCAGCATT 
CTGGTGCACA AGCTGCTGGA CAAGTTGTCG GTCACCTGTC CGCTCACGCC CTCCTGCTCG 
CTCAGCATGC CTCGCTGCGA CCTGGAGGCA CATCTCAAAC ACAGGTGTCC TGGAACACAG 
AGTCAGCGAA CAAAGCTAGA GCGGACGCAG ATGGAGGGAA GTGAGGACAG GGTCACAGCG 
ACCAATCCCC CCAAATCCCC CCAGACTGAG ATAAAGGAAG ACACGCCATC ACCACCTGCT 
GGATCCAATG CATGCAACAA TGTTCCTACA TGGACAGAAG AGCCTGGTAT AGACAACCCT 
GCCTTTGAAG AAAGCACAGA AGAAGACAGT GTTCATGGTC TTGAGTGTGC TCTTCCACGT 
GTGAAGCGTC CTCTCAGCAA CCCATGCATT CATCTTCTTC GCACTGGCAG TTCGGCTTCT 
TCAGGATGGG ACTTTGTTGA AACTCTTCCT CTTTCTGCTG AAGAAGGTTG TGTAAAACTT 
CCTTCTCTAC CTGAGGGAGA AATCACTACT ATTGAGGTTC ACCGTACAAA CCCCTACTCG 
GAGATGGGCA TAAGTATTGT GGGTGGAAAT GAGACTCCCC TGATAAATGT GGTAATACAG 
GAGGTGTACC GTGACGGTGT TATAGCCCGA GACGGAAGGC TGCTGGCCGG AGACCAAATA 
CTTCAGGTGA ACAATGTAGA CATCAGTAAC GTGCCCCATA ACTTTGCTCG CTCTACACTG 
GCCCGTCCCT GTGCTACCCT TCAGCTGACG GTTTTAAGAG AGCGCCGCTG CTCTGCCAGG 
CCACCTGCAG CAACTGCATC ACCCAAGGGC AGCCCTGCCA GCATACGCAT CACCCTGCAC 
AAGAGGGAAT CATCAGAGCA GCTGGGCATC AAGCTTGTGC GGCGTACAGA TGAAGCCGGC 
GTTTTTATTC TGGATCTGCT GGAGGGAGGC TTAGCTGCGA AAGATGGGCG ACTCTGCAGC 
AACGACAGGG TGCTGGCTGT CAATGAGCAT GACCTGAGAC ATGGCACGCC AGAGCTGGCA 
GCTCAGATTA TACAGGCTAG CGGAGAAAGA GTCAATCTGC TGATTAGCCG GTCCAGCAAG 
CAGACTATGG CGGTGCACAC AGGCTCCACC TTGACCAGGG ACATTTGGAG CCATGATCAC 
ATCCCGCCAC TTCCCAGTAC TGCGACCCCA AGTCCCGTCC CCAGTCTGCA CCTTGCCAGG 
TCTTCCACCC AGAGAGATCT CTCCCAGTGT GTGAACTGTA AAGAGAAACA CATCACTGTG 
AAGAAGGAGC CTCATGAGTC TCTGGGAATG ACTGTGGCAG GAGGACGGGG CAGCAAAAGT 
GGGGAACTGC CCATATTTGT GACGAGCGTC CAGCCCCATG GCTGCCTGTC ACGTGACGGA 
CGAATCAAAC GTGGGGATGT ACTGTTAAGT ATTAACGGTC AGGATTTGAC CTACCTGAGC 
CACAGCGAAG CAGTTGGCAC CCTGAAGTCC AGCGCCACGT CCTGCTCTGT TCAGCTGAAA 
GCACTAGAAG TCACCATGGT GGAAGAGCCA GGCCTGGACG AAGAGCTTCT CCCTCCACAC 
GAAAATGACT ACGATGCAAG CTGGTCGCCA TCATGGGTCT TGTGGCTTGG GCTTCCCAGC 
TACCTGCATA GCAGTCATGA GATCGTCCTC CGCAGGAGTC ACCCTGGCAG CTGGGGCTTC 
AGCATTGTTG GAGGTTATGA GGAGAACCAC AGCAACCAGG CATTCTTTAT CAAGACTATC 
GTCCTTGGAA CTCCCGCATA CTACGATGGT CGTCTAAAGT GTGGTGACAT GATAGTTGCT 
GTAAACGGTC TGTCCACAGC TGGCATGAGT CATTCCGCGC TTGTGCCCAT GCTTAAGGAG 
CAGCGGAGCA GGGTGGCGCT TACGGTTGTG TCCTGGCCAG GAAGTCTGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lnx2a ( XM_021470169.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325844.1 Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151691
Clone ID Related Accession (Same CDS sequence)	XM_021470169.1
Accession Version	XM_021470169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ligand of Numb protein X 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGAA ACTTCAACTC AAGAAACCTT TTCAACCACT TCTTCTGTGA AACTGCCAAG 
GGTAACCCAG AGCTATGGTG TCCTGGAACA CAGAGTCAGC GAACAAAGCT AGAGCGGACG 
CAGATGGAGG GAAGTGAGGA CAGGGTCACA GCGACCAATC CCCCCAAATC CCCCCAGACT 
GAGATAAAGG AAGACACGCC ATCACCACCT GCTGGATCCA ATGCATGCAA CAATGTTCCT 
ACATGGACAG AAGAGCCTGG TATAGACAAC CCTGCCTTTG AAGAAAGCAC AGAAGAAGAC 
AGTGTTCATG GTCTTGAGTG TGCTCTTCCA CGTGTGAAGC GTCCTCTCAG CAACCCATGC 
ATTCATCTTC TTCGCACTGG CAGTTCGGCT TCTTCAGGAT GGGACTTTGT TGAAACTCTT 
CCTCTTTCTG CTGAAGAAGG TTGTGTAAAA CTTCCTTCTC TACCTGAGGG AGAAATCACT 
ACTATTGAGG TTCACCGTAC AAACCCCTAC TCGGAGATGG GCATAAGTAT TGTGGGTGGA 
AATGAGACTC CCCTGATAAA TGTGGTAATA CAGGAGGTGT ACCGTGACGG TGTTATAGCC 
CGAGACGGAA GGCTGCTGGC CGGAGACCAA ATACTTCAGG TGAACAATGT AGACATCAGT 
AACGTGCCCC ATAACTTTGC TCGCTCTACA CTGGCCCGTC CCTGTGCTAC CCTTCAGCTG 
ACGGTTTTAA GAGAGCGCCG CTGCTCTGCC AGGCCACCTG CAGCAACTGC ATCACCCAAG 
GGCAGCCCTG CCAGCATACG CATCACCCTG CACAAGAGGG AATCATCAGA GCAGCTGGGC 
ATCAAGCTTG TGCGGCGTAC AGATGAAGCC GGCGTTTTTA TTCTGGATCT GCTGGAGGGA 
GGCTTAGCTG CGAAAGATGG GCGACTCTGC AGCAACGACA GGGTGCTGGC TGTCAATGAG 
CATGACCTGA GACATGGCAC GCCAGAGCTG GCAGCTCAGA TTATACAGGC TAGCGGAGAA 
AGAGTCAATC TGCTGATTAG CCGGTCCAGC AAGCAGACTA TGGCGGTGCA CACAGGCTCC 
ACCTTGACCA GGGACATTTG GAGCCATGAT CACATCCCGC CACTTCCCAG TACTGCGACC 
CCAAGTCCCG TCCCCAGTCT GCACCTTGCC AGGTCTTCCA CCCAGAGAGA TCTCTCCCAG 
TGTGTGAACT GTAAAGAGAA ACACATCACT GTGAAGAAGG AGCCTCATGA GTCTCTGGGA 
ATGACTGTGG CAGGAGGACG GGGCAGCAAA AGTGGGGAAC TGCCCATATT TGTGACGAGC 
GTCCAGCCCC ATGGCTGCCT GTCACGTGAC GGACGAATCA AACGTGGGGA TGTACTGTTA 
AGTATTAACG GTCAGGATTT GACCTACCTG AGCCACAGCG AAGCAGTTGG CACCCTGAAG 
TCCAGCGCCA CGTCCTGCTC TGTTCAGCTG AAAGCACTAG AAGTCACCAT GGTGGAAGAG 
CCAGGCCTGG ACGAAGAGCT TCTCCCTCCA CACGAAAATG ACTACGATGC AAGCTGGTCG 
CCATCATGGG TCTTGTGGCT TGGGCTTCCC AGCTACCTGC ATAGCAGTCA TGAGATCGTC 
CTCCGCAGGA GTCACCCTGG CAGCTGGGGC TTCAGCATTG TTGGAGGTTA TGAGGAGAAC 
CACAGCAACC AGGCATTCTT TATCAAGACT ATCGTCCTTG GAACTCCCGC ATACTACGAT 
GGTCGTCTAA AGTGTGGTGA CATGATAGTT GCTGTAAACG GTCTGTCCAC AGCTGGCATG 
AGTCATTCCG CGCTTGTGCC CATGCTTAAG GAGCAGCGGA GCAGGGTGGC GCTTACGGTT 
GTGTCCTGGC CAGGAAGTCT GATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325844.1
CDS	61..1947
Translation	MLGNFNSRNLFNHFFCETAKGNPELWCPGTQSQRTKLERTQMEGSEDRVTATNPPKSPQTEIKEDTPSPPAGSNACNNVPTWTEEPGIDNPAFEESTEEDSVHGLECALPRVKRPLSNPCIHLLRTGSSASSGWDFVETLPLSAEEGCVKLPSLPEGEITTIEVHRTNPYSEMGISIVGGNETPLINVVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNVPHNFARSTLARPCATLQLTVLRERRCSARPPAATASPKGSPASIRITLHKRESSEQLGIKLVRRTDEAGVFILDLLEGGLAAKDGRLCSNDRVLAVNEHDLRHGTPELAAQIIQASGERVNLLISRSSKQTMAVHTGSTLTRDIWSHDHIPPLPSTATPSPVPSLHLARSSTQRDLSQCVNCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINGQDLTYLSHSEAVGTLKSSATSCSVQLKALEVTMVEEPGLDEELLPPHENDYDASWSPSWVLWLGLPSYLHSSHEIVLRRSHPGSWGFSIVGGYEENHSNQAFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRVALTVVSWPGSLI

Target ORF information:

RefSeq Version	XM_021470169.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470169.1

ORF Insert Sequence:

ATGCTGGGAA ACTTCAACTC AAGAAACCTT TTCAACCACT TCTTCTGTGA AACTGCCAAG 
GGTAACCCAG AGCTATGGTG TCCTGGAACA CAGAGTCAGC GAACAAAGCT AGAGCGGACG 
CAGATGGAGG GAAGTGAGGA CAGGGTCACA GCGACCAATC CCCCCAAATC CCCCCAGACT 
GAGATAAAGG AAGACACGCC ATCACCACCT GCTGGATCCA ATGCATGCAA CAATGTTCCT 
ACATGGACAG AAGAGCCTGG TATAGACAAC CCTGCCTTTG AAGAAAGCAC AGAAGAAGAC 
AGTGTTCATG GTCTTGAGTG TGCTCTTCCA CGTGTGAAGC GTCCTCTCAG CAACCCATGC 
ATTCATCTTC TTCGCACTGG CAGTTCGGCT TCTTCAGGAT GGGACTTTGT TGAAACTCTT 
CCTCTTTCTG CTGAAGAAGG TTGTGTAAAA CTTCCTTCTC TACCTGAGGG AGAAATCACT 
ACTATTGAGG TTCACCGTAC AAACCCCTAC TCGGAGATGG GCATAAGTAT TGTGGGTGGA 
AATGAGACTC CCCTGATAAA TGTGGTAATA CAGGAGGTGT ACCGTGACGG TGTTATAGCC 
CGAGACGGAA GGCTGCTGGC CGGAGACCAA ATACTTCAGG TGAACAATGT AGACATCAGT 
AACGTGCCCC ATAACTTTGC TCGCTCTACA CTGGCCCGTC CCTGTGCTAC CCTTCAGCTG 
ACGGTTTTAA GAGAGCGCCG CTGCTCTGCC AGGCCACCTG CAGCAACTGC ATCACCCAAG 
GGCAGCCCTG CCAGCATACG CATCACCCTG CACAAGAGGG AATCATCAGA GCAGCTGGGC 
ATCAAGCTTG TGCGGCGTAC AGATGAAGCC GGCGTTTTTA TTCTGGATCT GCTGGAGGGA 
GGCTTAGCTG CGAAAGATGG GCGACTCTGC AGCAACGACA GGGTGCTGGC TGTCAATGAG 
CATGACCTGA GACATGGCAC GCCAGAGCTG GCAGCTCAGA TTATACAGGC TAGCGGAGAA 
AGAGTCAATC TGCTGATTAG CCGGTCCAGC AAGCAGACTA TGGCGGTGCA CACAGGCTCC 
ACCTTGACCA GGGACATTTG GAGCCATGAT CACATCCCGC CACTTCCCAG TACTGCGACC 
CCAAGTCCCG TCCCCAGTCT GCACCTTGCC AGGTCTTCCA CCCAGAGAGA TCTCTCCCAG 
TGTGTGAACT GTAAAGAGAA ACACATCACT GTGAAGAAGG AGCCTCATGA GTCTCTGGGA 
ATGACTGTGG CAGGAGGACG GGGCAGCAAA AGTGGGGAAC TGCCCATATT TGTGACGAGC 
GTCCAGCCCC ATGGCTGCCT GTCACGTGAC GGACGAATCA AACGTGGGGA TGTACTGTTA 
AGTATTAACG GTCAGGATTT GACCTACCTG AGCCACAGCG AAGCAGTTGG CACCCTGAAG 
TCCAGCGCCA CGTCCTGCTC TGTTCAGCTG AAAGCACTAG AAGTCACCAT GGTGGAAGAG 
CCAGGCCTGG ACGAAGAGCT TCTCCCTCCA CACGAAAATG ACTACGATGC AAGCTGGTCG 
CCATCATGGG TCTTGTGGCT TGGGCTTCCC AGCTACCTGC ATAGCAGTCA TGAGATCGTC 
CTCCGCAGGA GTCACCCTGG CAGCTGGGGC TTCAGCATTG TTGGAGGTTA TGAGGAGAAC 
CACAGCAACC AGGCATTCTT TATCAAGACT ATCGTCCTTG GAACTCCCGC ATACTACGAT 
GGTCGTCTAA AGTGTGGTGA CATGATAGTT GCTGTAAACG GTCTGTCCAC AGCTGGCATG 
AGTCATTCCG CGCTTGTGCC CATGCTTAAG GAGCAGCGGA GCAGGGTGGC GCTTACGGTT 
GTGTCCTGGC CAGGAAGTCT GATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lnx2a ( XM_021470170.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325845.1 Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa151692
Clone ID Related Accession (Same CDS sequence)	XM_021470170.1
Accession Version	XM_021470170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ligand of Numb protein X 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA GTGAGGACAG GGTCACAGCG ACCAATCCCC CCAAATCCCC CCAGACTGAG 
ATAAAGGAAG ACACGCCATC ACCACCTGCT GGATCCAATG CATGCAACAA TGTTCCTACA 
TGGACAGAAG AGCCTGGTAT AGACAACCCT GCCTTTGAAG AAAGCACAGA AGAAGACAGT 
GTTCATGGTC TTGAGTGTGC TCTTCCACGT GTGAAGCGTC CTCTCAGCAA CCCATGCATT 
CATCTTCTTC GCACTGGCAG TTCGGCTTCT TCAGGATGGG ACTTTGTTGA AACTCTTCCT 
CTTTCTGCTG AAGAAGGTTG TGTAAAACTT CCTTCTCTAC CTGAGGGAGA AATCACTACT 
ATTGAGGTTC ACCGTACAAA CCCCTACTCG GAGATGGGCA TAAGTATTGT GGGTGGAAAT 
GAGACTCCCC TGATAAATGT GGTAATACAG GAGGTGTACC GTGACGGTGT TATAGCCCGA 
GACGGAAGGC TGCTGGCCGG AGACCAAATA CTTCAGGTGA ACAATGTAGA CATCAGTAAC 
GTGCCCCATA ACTTTGCTCG CTCTACACTG GCCCGTCCCT GTGCTACCCT TCAGCTGACG 
GTTTTAAGAG AGCGCCGCTG CTCTGCCAGG CCACCTGCAG CAACTGCATC ACCCAAGGGC 
AGCCCTGCCA GCATACGCAT CACCCTGCAC AAGAGGGAAT CATCAGAGCA GCTGGGCATC 
AAGCTTGTGC GGCGTACAGA TGAAGCCGGC GTTTTTATTC TGGATCTGCT GGAGGGAGGC 
TTAGCTGCGA AAGATGGGCG ACTCTGCAGC AACGACAGGG TGCTGGCTGT CAATGAGCAT 
GACCTGAGAC ATGGCACGCC AGAGCTGGCA GCTCAGATTA TACAGGCTAG CGGAGAAAGA 
GTCAATCTGC TGATTAGCCG GTCCAGCAAG CAGACTATGG CGGTGCACAC AGGCTCCACC 
TTGACCAGGG ACATTTGGAG CCATGATCAC ATCCCGCCAC TTCCCAGTAC TGCGACCCCA 
AGTCCCGTCC CCAGTCTGCA CCTTGCCAGG TCTTCCACCC AGAGAGATCT CTCCCAGTGT 
GTGAACTGTA AAGAGAAACA CATCACTGTG AAGAAGGAGC CTCATGAGTC TCTGGGAATG 
ACTGTGGCAG GAGGACGGGG CAGCAAAAGT GGGGAACTGC CCATATTTGT GACGAGCGTC 
CAGCCCCATG GCTGCCTGTC ACGTGACGGA CGAATCAAAC GTGGGGATGT ACTGTTAAGT 
ATTAACGGTC AGGATTTGAC CTACCTGAGC CACAGCGAAG CAGTTGGCAC CCTGAAGTCC 
AGCGCCACGT CCTGCTCTGT TCAGCTGAAA GCACTAGAAG TCACCATGGT GGAAGAGCCA 
GGCCTGGACG AAGAGCTTCT CCCTCCACAC GAAAATGACT ACGATGCAAG CTGGTCGCCA 
TCATGGGTCT TGTGGCTTGG GCTTCCCAGC TACCTGCATA GCAGTCATGA GATCGTCCTC 
CGCAGGAGTC ACCCTGGCAG CTGGGGCTTC AGCATTGTTG GAGGTTATGA GGAGAACCAC 
AGCAACCAGG CATTCTTTAT CAAGACTATC GTCCTTGGAA CTCCCGCATA CTACGATGGT 
CGTCTAAAGT GTGGTGACAT GATAGTTGCT GTAAACGGTC TGTCCACAGC TGGCATGAGT 
CATTCCGCGC TTGTGCCCAT GCTTAAGGAG CAGCGGAGCA GGGTGGCGCT TACGGTTGTG 
TCCTGGCCAG GAAGTCTGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325845.1
CDS	92..1855
Translation	MEGSEDRVTATNPPKSPQTEIKEDTPSPPAGSNACNNVPTWTEEPGIDNPAFEESTEEDSVHGLECALPRVKRPLSNPCIHLLRTGSSASSGWDFVETLPLSAEEGCVKLPSLPEGEITTIEVHRTNPYSEMGISIVGGNETPLINVVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNVPHNFARSTLARPCATLQLTVLRERRCSARPPAATASPKGSPASIRITLHKRESSEQLGIKLVRRTDEAGVFILDLLEGGLAAKDGRLCSNDRVLAVNEHDLRHGTPELAAQIIQASGERVNLLISRSSKQTMAVHTGSTLTRDIWSHDHIPPLPSTATPSPVPSLHLARSSTQRDLSQCVNCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINGQDLTYLSHSEAVGTLKSSATSCSVQLKALEVTMVEEPGLDEELLPPHENDYDASWSPSWVLWLGLPSYLHSSHEIVLRRSHPGSWGFSIVGGYEENHSNQAFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRVALTVVSWPGSLI

Target ORF information:

RefSeq Version	XM_021470170.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470170.1

ORF Insert Sequence:

ATGGAGGGAA GTGAGGACAG GGTCACAGCG ACCAATCCCC CCAAATCCCC CCAGACTGAG 
ATAAAGGAAG ACACGCCATC ACCACCTGCT GGATCCAATG CATGCAACAA TGTTCCTACA 
TGGACAGAAG AGCCTGGTAT AGACAACCCT GCCTTTGAAG AAAGCACAGA AGAAGACAGT 
GTTCATGGTC TTGAGTGTGC TCTTCCACGT GTGAAGCGTC CTCTCAGCAA CCCATGCATT 
CATCTTCTTC GCACTGGCAG TTCGGCTTCT TCAGGATGGG ACTTTGTTGA AACTCTTCCT 
CTTTCTGCTG AAGAAGGTTG TGTAAAACTT CCTTCTCTAC CTGAGGGAGA AATCACTACT 
ATTGAGGTTC ACCGTACAAA CCCCTACTCG GAGATGGGCA TAAGTATTGT GGGTGGAAAT 
GAGACTCCCC TGATAAATGT GGTAATACAG GAGGTGTACC GTGACGGTGT TATAGCCCGA 
GACGGAAGGC TGCTGGCCGG AGACCAAATA CTTCAGGTGA ACAATGTAGA CATCAGTAAC 
GTGCCCCATA ACTTTGCTCG CTCTACACTG GCCCGTCCCT GTGCTACCCT TCAGCTGACG 
GTTTTAAGAG AGCGCCGCTG CTCTGCCAGG CCACCTGCAG CAACTGCATC ACCCAAGGGC 
AGCCCTGCCA GCATACGCAT CACCCTGCAC AAGAGGGAAT CATCAGAGCA GCTGGGCATC 
AAGCTTGTGC GGCGTACAGA TGAAGCCGGC GTTTTTATTC TGGATCTGCT GGAGGGAGGC 
TTAGCTGCGA AAGATGGGCG ACTCTGCAGC AACGACAGGG TGCTGGCTGT CAATGAGCAT 
GACCTGAGAC ATGGCACGCC AGAGCTGGCA GCTCAGATTA TACAGGCTAG CGGAGAAAGA 
GTCAATCTGC TGATTAGCCG GTCCAGCAAG CAGACTATGG CGGTGCACAC AGGCTCCACC 
TTGACCAGGG ACATTTGGAG CCATGATCAC ATCCCGCCAC TTCCCAGTAC TGCGACCCCA 
AGTCCCGTCC CCAGTCTGCA CCTTGCCAGG TCTTCCACCC AGAGAGATCT CTCCCAGTGT 
GTGAACTGTA AAGAGAAACA CATCACTGTG AAGAAGGAGC CTCATGAGTC TCTGGGAATG 
ACTGTGGCAG GAGGACGGGG CAGCAAAAGT GGGGAACTGC CCATATTTGT GACGAGCGTC 
CAGCCCCATG GCTGCCTGTC ACGTGACGGA CGAATCAAAC GTGGGGATGT ACTGTTAAGT 
ATTAACGGTC AGGATTTGAC CTACCTGAGC CACAGCGAAG CAGTTGGCAC CCTGAAGTCC 
AGCGCCACGT CCTGCTCTGT TCAGCTGAAA GCACTAGAAG TCACCATGGT GGAAGAGCCA 
GGCCTGGACG AAGAGCTTCT CCCTCCACAC GAAAATGACT ACGATGCAAG CTGGTCGCCA 
TCATGGGTCT TGTGGCTTGG GCTTCCCAGC TACCTGCATA GCAGTCATGA GATCGTCCTC 
CGCAGGAGTC ACCCTGGCAG CTGGGGCTTC AGCATTGTTG GAGGTTATGA GGAGAACCAC 
AGCAACCAGG CATTCTTTAT CAAGACTATC GTCCTTGGAA CTCCCGCATA CTACGATGGT 
CGTCTAAAGT GTGGTGACAT GATAGTTGCT GTAAACGGTC TGTCCACAGC TGGCATGAGT 
CATTCCGCGC TTGTGCCCAT GCTTAAGGAG CAGCGGAGCA GGGTGGCGCT TACGGTTGTG 
TCCTGGCCAG GAAGTCTGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lnx2a ( XM_021470171.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325846.1 Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X3, mRNA.

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CloneID	ODa151693
Clone ID Related Accession (Same CDS sequence)	XM_021470171.1
Accession Version	XM_021470171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ligand of Numb protein X 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGTC TGGGAGAGGG AGTGGACCCG GGCATGGTGA GCGAAGCTCT TCTGGAGGCC 
CTTTGCTCTG AATGTGGACA GATTCACCGC ACCTGGGAGA ACCACCTTTA TAACTATCGT 
TTGGAAGTGG ATGATGATTT GGTGTGCCAC ATCTGCCTTC AGCCGTTAGT TCAGCCCCTG 
GATACACCAT GTGGCCACAC CTTCTGTGCC CGCTGTCTGC GCAGCTTCCT GCAGGAACGG 
GACTTCTGTC CACTGGACCG TGCGCATCTG CAGCTGCAGG TCTGCCGGCG CTCCAGCATT 
CTGGTGCACA AGCTGCTGGA CAAGTTGTCG GTCACCTGTC CGCTCACGCC CTCCTGCTCG 
CTCAGCATGC CTCGCTGCGA CCTGGAGGCA CATCTCAAAC ACAGGTGTCC TGGAACACAG 
AGTCAGCGAA CAAAGCTAGA GCGGACGCAG ATGGAGGGAA GTGAGGACAG GGTCACAGCG 
ACCAATCCCC CCAAATCCCC CCAGACTGAG ATAAAGGAAG ACACGCCATC ACCACCTGCT 
GGATCCAATG CATGCAACAA TGTTCCTACA TGGACAGAAG AGCCTGGTAT AGACAACCCT 
GCCTTTGAAG AAAGCACAGA AGAAGACAGT GTTCATGGTC TTGAGTGTGC TCTTCCACGT 
GTGAAGCGTC CTCTCAGCAA CCCATGCATT CATCTTCTTC GCACTGGCAG TTCGGCTTCT 
TCAGGATGGG ACTTTGTTGA AACTCTTCCT CTTTCTGCTG AAGAAGGTTG TGTAAAACTT 
CCTTCTCTAC CTGAGGGAGA AATCACTACT ATTGAGGTTC ACCGTACAAA CCCCTACTCG 
GAGATGGGCA TAAGTATTGT GGGTGGAAAT GAGACTCCCC TGATAAATGT GGTAATACAG 
GAGGTGTACC GTGACGGTGT TATAGCCCGA GACGGAAGGC TGCTGGCCGG AGACCAAATA 
CTTCAGGTGA ACAATGTAGA CATCAGTAAC GTGCCCCATA ACTTTGCTCG CTCTACACTG 
GCCCGTCCCT GTGCTACCCT TCAGCTGACG GTTTTAAGAG AGCGCCGCTG CTCTGCCAGG 
CCACCTGCAG CAACTGCATC ACCCAAGGGC AGCCCTGCCA GCATACGCAT CACCCTGCAC 
AAGAGGGAAT CATCAGAGCA GCTGGGCATC AAGCTTGTGC GGCGTACAGA TGAAGCCGGC 
GTTTTTATTC TGGATCTGCT GGAGGGAGGC TTAGCTGCGA AAGATGGGCG ACTCTGCAGC 
AACGACAGGG TGCTGGCTGT CAATGAGCAT GACCTGAGAC ATGGCACGCC AGAGCTGGCA 
GCTCAGATTA TACAGGCTAG CGGAGAAAGA GTCAATCTGC TGATTAGCCG GTCCAGCAAG 
CAGACTATGG CGGTGCACAC AGGCTCCACC TTGACCAGGG ACATTTGGAG CCATGATCAC 
ATCCCGCCAC TTCCCAGTAC TGCGACCCCA AGTCCCGTCC CCAGTCTGCA CCTTGCCAGG 
TCTTCCACCC AGAGAGATCT CTCCCAGTGT GTGAACTGTA AAGAGAAACA CATCACTGTG 
AAGAAGGAGC CTCATGAGTC TCTGGGAATG ACTGTGGCAG GAGGACGGGG CAGCAAAAGT 
GGGGAACTGC CCATATTTGT GACGAGCGTC CAGCCCCATG GCTGCCTGTC ACGTGACGGA 
CGAATCAAAC GTGGAGGACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325846.1
CDS	247..1950
Translation	MGSLGEGVDPGMVSEALLEALCSECGQIHRTWENHLYNYRLEVDDDLVCHICLQPLVQPLDTPCGHTFCARCLRSFLQERDFCPLDRAHLQLQVCRRSSILVHKLLDKLSVTCPLTPSCSLSMPRCDLEAHLKHRCPGTQSQRTKLERTQMEGSEDRVTATNPPKSPQTEIKEDTPSPPAGSNACNNVPTWTEEPGIDNPAFEESTEEDSVHGLECALPRVKRPLSNPCIHLLRTGSSASSGWDFVETLPLSAEEGCVKLPSLPEGEITTIEVHRTNPYSEMGISIVGGNETPLINVVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNVPHNFARSTLARPCATLQLTVLRERRCSARPPAATASPKGSPASIRITLHKRESSEQLGIKLVRRTDEAGVFILDLLEGGLAAKDGRLCSNDRVLAVNEHDLRHGTPELAAQIIQASGERVNLLISRSSKQTMAVHTGSTLTRDIWSHDHIPPLPSTATPSPVPSLHLARSSTQRDLSQCVNCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGGL

Target ORF information:

RefSeq Version	XM_021470171.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ligand of numb-protein X 2a (lnx2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470171.1

ORF Insert Sequence:

ATGGGCAGTC TGGGAGAGGG AGTGGACCCG GGCATGGTGA GCGAAGCTCT TCTGGAGGCC 
CTTTGCTCTG AATGTGGACA GATTCACCGC ACCTGGGAGA ACCACCTTTA TAACTATCGT 
TTGGAAGTGG ATGATGATTT GGTGTGCCAC ATCTGCCTTC AGCCGTTAGT TCAGCCCCTG 
GATACACCAT GTGGCCACAC CTTCTGTGCC CGCTGTCTGC GCAGCTTCCT GCAGGAACGG 
GACTTCTGTC CACTGGACCG TGCGCATCTG CAGCTGCAGG TCTGCCGGCG CTCCAGCATT 
CTGGTGCACA AGCTGCTGGA CAAGTTGTCG GTCACCTGTC CGCTCACGCC CTCCTGCTCG 
CTCAGCATGC CTCGCTGCGA CCTGGAGGCA CATCTCAAAC ACAGGTGTCC TGGAACACAG 
AGTCAGCGAA CAAAGCTAGA GCGGACGCAG ATGGAGGGAA GTGAGGACAG GGTCACAGCG 
ACCAATCCCC CCAAATCCCC CCAGACTGAG ATAAAGGAAG ACACGCCATC ACCACCTGCT 
GGATCCAATG CATGCAACAA TGTTCCTACA TGGACAGAAG AGCCTGGTAT AGACAACCCT 
GCCTTTGAAG AAAGCACAGA AGAAGACAGT GTTCATGGTC TTGAGTGTGC TCTTCCACGT 
GTGAAGCGTC CTCTCAGCAA CCCATGCATT CATCTTCTTC GCACTGGCAG TTCGGCTTCT 
TCAGGATGGG ACTTTGTTGA AACTCTTCCT CTTTCTGCTG AAGAAGGTTG TGTAAAACTT 
CCTTCTCTAC CTGAGGGAGA AATCACTACT ATTGAGGTTC ACCGTACAAA CCCCTACTCG 
GAGATGGGCA TAAGTATTGT GGGTGGAAAT GAGACTCCCC TGATAAATGT GGTAATACAG 
GAGGTGTACC GTGACGGTGT TATAGCCCGA GACGGAAGGC TGCTGGCCGG AGACCAAATA 
CTTCAGGTGA ACAATGTAGA CATCAGTAAC GTGCCCCATA ACTTTGCTCG CTCTACACTG 
GCCCGTCCCT GTGCTACCCT TCAGCTGACG GTTTTAAGAG AGCGCCGCTG CTCTGCCAGG 
CCACCTGCAG CAACTGCATC ACCCAAGGGC AGCCCTGCCA GCATACGCAT CACCCTGCAC 
AAGAGGGAAT CATCAGAGCA GCTGGGCATC AAGCTTGTGC GGCGTACAGA TGAAGCCGGC 
GTTTTTATTC TGGATCTGCT GGAGGGAGGC TTAGCTGCGA AAGATGGGCG ACTCTGCAGC 
AACGACAGGG TGCTGGCTGT CAATGAGCAT GACCTGAGAC ATGGCACGCC AGAGCTGGCA 
GCTCAGATTA TACAGGCTAG CGGAGAAAGA GTCAATCTGC TGATTAGCCG GTCCAGCAAG 
CAGACTATGG CGGTGCACAC AGGCTCCACC TTGACCAGGG ACATTTGGAG CCATGATCAC 
ATCCCGCCAC TTCCCAGTAC TGCGACCCCA AGTCCCGTCC CCAGTCTGCA CCTTGCCAGG 
TCTTCCACCC AGAGAGATCT CTCCCAGTGT GTGAACTGTA AAGAGAAACA CATCACTGTG 
AAGAAGGAGC CTCATGAGTC TCTGGGAATG ACTGTGGCAG GAGGACGGGG CAGCAAAAGT 
GGGGAACTGC CCATATTTGT GACGAGCGTC CAGCCCCATG GCTGCCTGTC ACGTGACGGA 
CGAATCAAAC GTGGAGGACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PCR artifact in testing for homologous recombination in genomic editing in zebrafish.
PloS one12(3)e0172802(2017)
Won M,Dawid IB

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas.
Proceedings of the National Academy of Sciences of the United States of America112(40)12426-31(2015 Oct)
Won M,Ro H,Dawid IB

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lnx2a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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