crybb2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crybb2
Entrez Gene ID	553182
Full Name	crystallin, beta B2
General protein information	Preferred Names crystallin, beta B2 Names beta-crystallin B2 beta B2-crystallin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018128.1	NP_001018138.1	beta-crystallin B2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CRYBB2	NP_777232.1
Danio rerio (zebrafish)	crybb2	NP_001018138.1
Homo sapiens (human)	CRYBB2	NP_000487.1
Canis lupus familiaris (dog)	CRYBB2	NP_001041578.1
Mus musculus (house mouse)	Crybb2	NP_031799.1
Rattus norvegicus (Norway rat)	Crybb2	NP_037069.1
Gallus gallus (chicken)	CRYBB2	NP_990506.2
Pan troglodytes (chimpanzee)	CRYBB2	XP_003317196.1
	CRYBB2	NP_001116366.1
Xenopus tropicalis (tropical clawed frog)	crybb2	NP_001238879.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of proteomic profiles in the zebrafish retina during experimental degeneration and regeneration.
Eastlake K, Heywood WE, Tracey-White D, Aquino E, Bliss E, Vasta GR, Mills K, Khaw PT, Moosajee M, Limb GA
Scientific reports744601(2017 Mar)

Lens defects in Astyanax mexicanus Cavefish: evolution of crystallins and a role for alphaA-crystallin.
Hinaux H, Blin M, Fumey J, Legendre L, Heuz? A, Casane D, R?taux S
Developmental neurobiology75(5)505-21(2015 May)

Changes in zebrafish (Danio rerio) lens crystallin content during development.
Wages P, Horwitz J, Ding L, Corbin RW, Posner M
Molecular vision19408-17(2013)

The zebrafish lens proteome during development and aging.
Greiling TM, Houck SA, Clark JI
Molecular vision152313-25(2009 Nov)

A proteome map of the zebrafish (Danio rerio) lens reveals similarities between zebrafish and mammalian crystallin expression.
Posner M, Hawke M, Lacava C, Prince CJ, Bellanco NR, Corbin RW
Molecular vision14806-14(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crybb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crybb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35491	NM_001018128.1 Latest version!	Danio rerio crystallin, beta B2 (crybb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crybb2 ( NM_001018128.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018138.1 Danio rerio crystallin, beta B2 (crybb2), mRNA.

Price & Availability↑

CloneID	ODa35491
Clone ID Related Accession (Same CDS sequence)	NM_001018128.1
Accession Version	NM_001018128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Danio rerio(zebrafish)
Product	beta-crystallin B2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154625.1. On Sep 9, 2008 this sequence version replaced XM_693235.3. ##Evidence-Data-START## Transcript exon combination :: BC154625.1, EH456887.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505373, SAMEA3505374 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACTG ATCACCAGAA TCCTGCAACC AAGCAAAAAC AACCAGTTGC CAGTGCCTTC 
AAGTTGGTGA TCTACGAACA GGAAAACTTT CAAGGCCGCT GTCATGAGCT GACTGGGCCC 
TGTAACAACC TCCAGGAGGC GGGCGTGGAG AAAGTGGGCT CAGTACTGGT GCAGTGTGGA 
CCGTGGGTGG GATTTGAGCA GCCCGGCTGT AAGGGGGAAC AGTATGTGTT TGAGAAGGGT 
GAGTATCCTC GCTGGGACTC CTGGACCAAC AGCAGACGCA GTGACTGCAT CGTCGCCTTC 
CGCCCCATCA AAGTGGACAG CCAGGAACAC AAGATCGTCC TCTACGAGAA CCCAAGCTTC 
ACTGGGAAGA AGATTGAGAT CATAGACGAT GACGTTCCCA GCTTCCACGC TCATGGATAC 
CATGAGAAAG TCTCTTCAGT TCGTGTGCAG AGTGGCACCT GGGTGGGCTA TCAATACCCA 
GGATACAGAG GCTTCCAGTA TTTGTTTGAG AAAGGAGAGT TTAAGGAGTG TTCTGAGTTC 
GGGGCCGCTC TGCCTCAGAT CCAATCGGTG AGACGCATCC GGGACATGCA GTATCACCCT 
CGTGGAGCCT TTCAGGCCAC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018138.1
CDS	189..815
Translation	MATDHQNPATKQKQPVASAFKLVIYEQENFQGRCHELTGPCNNLQEAGVEKVGSVLVQCGPWVGFEQPGCKGEQYVFEKGEYPRWDSWTNSRRSDCIVAFRPIKVDSQEHKIVLYENPSFTGKKIEIIDDDVPSFHAHGYHEKVSSVRVQSGTWVGYQYPGYRGFQYLFEKGEFKECSEFGAALPQIQSVRRIRDMQYHPRGAFQATS

Target ORF information:

RefSeq Version	NM_001018128.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio crystallin, beta B2 (crybb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018128.1

ORF Insert Sequence:

ATGGCAACTG ATCACCAGAA TCCTGCAACC AAGCAAAAAC AACCAGTTGC CAGTGCCTTC 
AAGTTGGTGA TCTACGAACA GGAAAACTTT CAAGGCCGCT GTCATGAGCT GACTGGGCCC 
TGTAACAACC TCCAGGAGGC GGGCGTGGAG AAAGTGGGCT CAGTACTGGT GCAGTGTGGA 
CCGTGGGTGG GATTTGAGCA GCCCGGCTGT AAGGGGGAAC AGTATGTGTT TGAGAAGGGT 
GAGTATCCTC GCTGGGACTC CTGGACCAAC AGCAGACGCA GTGACTGCAT CGTCGCCTTC 
CGCCCCATCA AAGTGGACAG CCAGGAACAC AAGATCGTCC TCTACGAGAA CCCAAGCTTC 
ACTGGGAAGA AGATTGAGAT CATAGACGAT GACGTTCCCA GCTTCCACGC TCATGGATAC 
CATGAGAAAG TCTCTTCAGT TCGTGTGCAG AGTGGCACCT GGGTGGGCTA TCAATACCCA 
GGATACAGAG GCTTCCAGTA TTTGTTTGAG AAAGGAGAGT TTAAGGAGTG TTCTGAGTTC 
GGGGCCGCTC TGCCTCAGAT CCAATCGGTG AGACGCATCC GGGACATGCA GTATCACCCT 
CGTGGAGCCT TTCAGGCCAC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparison of proteomic profiles in the zebrafish retina during experimental degeneration and regeneration.
Scientific reports744601(2017 Mar)
Eastlake K,Heywood WE,Tracey-White D,Aquino E,Bliss E,Vasta GR,Mills K,Khaw PT,Moosajee M,Limb GA

Lens defects in Astyanax mexicanus Cavefish: evolution of crystallins and a role for alphaA-crystallin.
Developmental neurobiology75(5)505-21(2015 May)
Hinaux H,Blin M,Fumey J,Legendre L,Heuz? A,Casane D,R?taux S

Changes in zebrafish (Danio rerio) lens crystallin content during development.
Molecular vision19408-17(2013)
Wages P,Horwitz J,Ding L,Corbin RW,Posner M

The zebrafish lens proteome during development and aging.
Molecular vision152313-25(2009 Nov)
Greiling TM,Houck SA,Clark JI

A proteome map of the zebrafish (Danio rerio) lens reveals similarities between zebrafish and mammalian crystallin expression.
Molecular vision14806-14(2008 Apr)
Posner M,Hawke M,Lacava C,Prince CJ,Bellanco NR,Corbin RW