sppl3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sppl3
Entrez Gene ID	548609
Full Name	signal peptide peptidase 3
Synonyms	fb94d08,wu:fb94d08,zgc:114093
General protein information	Preferred Names signal peptide peptidase 3 Names signal peptide peptidase-like 3 signal peptide peptidase-like protein 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001015068.1	NP_001015068.1	signal peptide peptidase-like 3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPPL3	XP_002694571.1
Rattus norvegicus (Norway rat)	Sppl3	XP_003751248.1
Drosophila melanogaster (fruit fly)	SppL	NP_651437.1
Oryza sativa (rice)	Os10g0393100	NP_001064518.1
Xenopus tropicalis (tropical clawed frog)	sppl3	NP_988942.1
Canis lupus familiaris (dog)	SPPL3	XP_543427.2
Mus musculus (house mouse)	Sppl3	NP_083288.2
Pan troglodytes (chimpanzee)	SPPL3	NP_001233544.1
Danio rerio (zebrafish)	sppl3	NP_001015068.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003207	XP_312914.4
Arabidopsis thaliana (thale cress)	SPPL1	NP_567918.1
Homo sapiens (human)	SPPL3	NP_620584.2
	SPPL3	XP_001089218.1
Gallus gallus (chicken)	SPPL3	XP_004934435.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets.
Kal?n M, Wallgard E, Asker N, Nasevicius A, Athley E, Billgren E, Larson JD, Wadman SA, Norseng E, Clark KJ, He L, Karlsson-Lindahl L, H?ger AK, Weber H, Augustin H, Samuelsson T, Kemmet CK, Utesch CM, Essner JJ, Hackett PB, Hellstr?m M
Chemistry & biology16(4)432-41(2009 Apr)

Cellular functions of gamma-secretase-related proteins.
Haffner C, Haass C
Neuro-degenerative diseases3(4-5)284-9(2006)

Differential localization and identification of a critical aspartate suggest non-redundant proteolytic functions of the presenilin homologues SPPL2b and SPPL3.
Krawitz P, Haffner C, Fluhrer R, Steiner H, Schmid B, Haass C
The Journal of biological chemistry280(47)39515-23(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPPL2b is targeted through the secretory pathway to endosomes/lysosomes, whereas SPP and SPPL3 are restricted to the ER.
Title: Differential localization and identification of a critical aspartate suggest non-redundant proteolytic functions of the presenilin homologues SPPL2b and SPPL3.

The following sppl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sppl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32700	NM_001015068.1 Latest version!	Danio rerio signal peptide peptidase 3 (sppl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sppl3 ( NM_001015068.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001015068.1 Danio rerio signal peptide peptidase 3 (sppl3), mRNA.

Price & Availability↑

CloneID	ODa32700
Clone ID Related Accession (Same CDS sequence)	NM_001015068.1
Accession Version	NM_001015068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	signal peptide peptidase-like 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY926459.1. ##Evidence-Data-START## Transcript exon combination :: AY926459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC AGAGCTACTC CTGGGCGTAC TCCCTTGTCG ACTCCAGCCA GGTGTCAACC 
TTCCTCATCT CCATCCTCCT CATCGTCTAT GGCAGCTTCA GGTCATTAAA CATGGACTGC 
GAGAACCAGG AGAAAGACAA GGATGGAAAC CCCACCGCCA GTGGCTCCTT CAACAATGCC 
AACTCTAACA ACAGTATTCA GACCATAGAC TCCACACAGG CGCTGTTCCT GCCAATCGGA 
GCCTCCGTGT CTCTGCTCGT CATGTTTTTC TTCTTTGACT CGGTTCAGGT GGTCTTCACC 
ATATGCACTG CAGTTCTAGC AACCATTGCA TTTGCGTTCC TTCTGCTGCC GATGTGCCAG 
TATCTGACCA GACCCTGCTC CCCACAAAAC AAGATCTCAT TCGGCTGCTG TGGCCGCTTC 
ACGTTGGCGG AGCTGCTGTC GTTTTCGCTC TCTGTCATGC TGGTTCTCAT CTGGGTGCTG 
ACGGGCCACT GGCTGCTCAT GGACGCTCTT GCCATGGGTC TCTGCGTGGC TATGATTGCG 
TTTGTGAGGC TGCCGAGTCT GAAGGTGTCG TGTTTACTGC TGTCAGGCCT GCTCATCTAT 
GATGTTTTCT GGGTCTTCTT CTCCGCCTAC ATCTTCAACA GCAACGTGAT GGTGAAAGTG 
GCCACACAGC CTGCAGACAA CCCACTGGAC GTGCTGTCAC GCAAACTTCA CCTGGGGCCT 
GGCATGGGTC GGGACGTTCC CCGTCTCTCG CTGCCCGGCA AGTTGGTGTT TCCTAGCTCC 
ACCGGCAGCC ACTTCTCCAT GCTGGGCATC GGCGATATCG TCATGCCAGG CCTGCTGCTG 
TGTTTCGTGC TGCGATATGA CAACTATAAA AAACAGGCCA CCGGCGAAGT GCCCGGCCCT 
GCCAACATGT CAGGCCGCAT GCAGAGGGTT TCCTACTTCC ACTGCACCCT CATCGGTTAC 
TTTGTTGGTC TGTTGACTGC AACCGTGGCC TCTCGTATTC ACCGCGCGGC CCAGCCTGCT 
CTCCTGTACC TGGTGCCCTT CACCCTGCTG CCTCTGCTCA CTATGGCCTA CCTGAAGGGG 
GACCTGCGGC GCATGTGGTC CGAGCCCTTT CACGCCAAAT CCAGCAGCTC CCGCTTCCTG 
GAGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015068.1
CDS	38..1186
Translation	MAEQSYSWAYSLVDSSQVSTFLISILLIVYGSFRSLNMDCENQEKDKDGNPTASGSFNNANSNNSIQTIDSTQALFLPIGASVSLLVMFFFFDSVQVVFTICTAVLATIAFAFLLLPMCQYLTRPCSPQNKISFGCCGRFTLAELLSFSLSVMLVLIWVLTGHWLLMDALAMGLCVAMIAFVRLPSLKVSCLLLSGLLIYDVFWVFFSAYIFNSNVMVKVATQPADNPLDVLSRKLHLGPGMGRDVPRLSLPGKLVFPSSTGSHFSMLGIGDIVMPGLLLCFVLRYDNYKKQATGEVPGPANMSGRMQRVSYFHCTLIGYFVGLLTATVASRIHRAAQPALLYLVPFTLLPLLTMAYLKGDLRRMWSEPFHAKSSSSRFLEV

Target ORF information:

RefSeq Version	NM_001015068.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio signal peptide peptidase 3 (sppl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015068.1

ORF Insert Sequence:

ATGGCGGAGC AGAGCTACTC CTGGGCGTAC TCCCTTGTCG ACTCCAGCCA GGTGTCAACC 
TTCCTCATCT CCATCCTCCT CATCGTCTAT GGCAGCTTCA GGTCATTAAA CATGGACTGC 
GAGAACCAGG AGAAAGACAA GGATGGAAAC CCCACCGCCA GTGGCTCCTT CAACAATGCC 
AACTCTAACA ACAGTATTCA GACCATAGAC TCCACACAGG CGCTGTTCCT GCCAATCGGA 
GCCTCCGTGT CTCTGCTCGT CATGTTTTTC TTCTTTGACT CGGTTCAGGT GGTCTTCACC 
ATATGCACTG CAGTTCTAGC AACCATTGCA TTTGCGTTCC TTCTGCTGCC GATGTGCCAG 
TATCTGACCA GACCCTGCTC CCCACAAAAC AAGATCTCAT TCGGCTGCTG TGGCCGCTTC 
ACGTTGGCGG AGCTGCTGTC GTTTTCGCTC TCTGTCATGC TGGTTCTCAT CTGGGTGCTG 
ACGGGCCACT GGCTGCTCAT GGACGCTCTT GCCATGGGTC TCTGCGTGGC TATGATTGCG 
TTTGTGAGGC TGCCGAGTCT GAAGGTGTCG TGTTTACTGC TGTCAGGCCT GCTCATCTAT 
GATGTTTTCT GGGTCTTCTT CTCCGCCTAC ATCTTCAACA GCAACGTGAT GGTGAAAGTG 
GCCACACAGC CTGCAGACAA CCCACTGGAC GTGCTGTCAC GCAAACTTCA CCTGGGGCCT 
GGCATGGGTC GGGACGTTCC CCGTCTCTCG CTGCCCGGCA AGTTGGTGTT TCCTAGCTCC 
ACCGGCAGCC ACTTCTCCAT GCTGGGCATC GGCGATATCG TCATGCCAGG CCTGCTGCTG 
TGTTTCGTGC TGCGATATGA CAACTATAAA AAACAGGCCA CCGGCGAAGT GCCCGGCCCT 
GCCAACATGT CAGGCCGCAT GCAGAGGGTT TCCTACTTCC ACTGCACCCT CATCGGTTAC 
TTTGTTGGTC TGTTGACTGC AACCGTGGCC TCTCGTATTC ACCGCGCGGC CCAGCCTGCT 
CTCCTGTACC TGGTGCCCTT CACCCTGCTG CCTCTGCTCA CTATGGCCTA CCTGAAGGGG 
GACCTGCGGC GCATGTGGTC CGAGCCCTTT CACGCCAAAT CCAGCAGCTC CCGCTTCCTG 
GAGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets.
Chemistry & biology16(4)432-41(2009 Apr)
Kal?n M,Wallgard E,Asker N,Nasevicius A,Athley E,Billgren E,Larson JD,Wadman SA,Norseng E,Clark KJ,He L,Karlsson-Lindahl L,H?ger AK,Weber H,Augustin H,Samuelsson T,Kemmet CK,Utesch CM,Essner JJ,Hackett PB,Hellstr?m M

Cellular functions of gamma-secretase-related proteins.
Neuro-degenerative diseases3(4-5)284-9(2006)
Haffner C,Haass C

Differential localization and identification of a critical aspartate suggest non-redundant proteolytic functions of the presenilin homologues SPPL2b and SPPL3.
The Journal of biological chemistry280(47)39515-23(2005 Nov)
Krawitz P,Haffner C,Fluhrer R,Steiner H,Schmid B,Haass C