rbx1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rbx1
Entrez Gene ID	449846
Full Name	ring-box 1, E3 ubiquitin protein ligase
Synonyms	im:7137515,zgc:136444
General protein information	Preferred Names ring-box 1, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase RBX1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083544.1	NP_001077013.1	E3 ubiquitin-protein ligase RBX1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RBX1	NP_055063.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005202	XP_001688524.1
Arabidopsis thaliana (thale cress)	RBX1	NP_001154725.1
Xenopus tropicalis (tropical clawed frog)	rbx1	XP_002934772.2
Pan troglodytes (chimpanzee)	RBX1	XP_515153.2
Canis lupus familiaris (dog)	RBX1	XP_531722.2
Mus musculus (house mouse)	Gm9840	XP_006525295.1
Danio rerio (zebrafish)	rbx1	NP_001077013.1
Bos taurus (cattle)	RBX1	NP_001039706.1
Drosophila melanogaster (fruit fly)	Roc1a	NP_569852.1
Magnaporthe oryzae (rice blast fungus)	MGG_08844	XP_003713864.1
Gallus gallus (chicken)	RBX1	NP_001186179.1
Schizosaccharomyces pombe (fission yeast)	rbx1	NP_593388.1
	LOC710252	XP_001098854.2
Caenorhabditis elegans (roundworm)	rbx-1	NP_505496.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive, individual zebrafish embryos.
Rauwerda H, Pagano JF, de Leeuw WC, Ensink W, Nehrdich U, de Jong M, Jonker M, Spaink HP, Breit TM
BMC genomics18(1)287(2017 04)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Transcriptional events co-regulated by hypoxia and cold stresses in Zebrafish larvae.
Long Y, Yan J, Song G, Li X, Li X, Li Q, Cui Z
BMC genomics16385(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rbx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rbx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35574	NM_001083544.1 Latest version!	Danio rerio ring-box 1, E3 ubiquitin protein ligase (rbx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rbx1 ( NM_001083544.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077013.1 Danio rerio ring-box 1, E3 ubiquitin protein ligase (rbx1), mRNA.

Price & Availability↑

CloneID	ODa35574
Clone ID Related Accession (Same CDS sequence)	NM_001083544.1
Accession Version	NM_001083544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase RBX1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX470254.7. On Jan 22, 2011 this sequence version replaced XM_688824.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: EH545080.1, EH566212.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505373, SAMEA3505378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CGATGGATGT GGACACCCCA AGTGGAGCTA ACAGCGGAGC CAGTAAAAAG 
CGATTTGAAG TGAAAAAGTG GAACGCTGTG GCTCTGTGGG CATGGGACAT TGTAGTGGAC 
AACTGTGCCA TCTGCAGAAA TCACATTATG GACCTCTGTA TTGAGTGTCA GGCCAATCAG 
GCGTCTGCCA CCTCAGAGGA GTGTACGGTG GCCTGGGGAG TCTGCAATCA CGCTTTTCAT 
TTCCACTGCA TCTCCCGCTG GCTGAAGACC AGACAAGTGT GTCCACTGGA CAACAGAGAG 
TGGGAGTTTC AGAAGTATGG TCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077013.1
CDS	36..362
Translation	MAAAMDVDTPSGANSGASKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH

Target ORF information:

RefSeq Version	NM_001083544.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ring-box 1, E3 ubiquitin protein ligase (rbx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083544.1

ORF Insert Sequence:

ATGGCGGCCG CGATGGATGT GGACACCCCA AGTGGAGCTA ACAGCGGAGC CAGTAAAAAG 
CGATTTGAAG TGAAAAAGTG GAACGCTGTG GCTCTGTGGG CATGGGACAT TGTAGTGGAC 
AACTGTGCCA TCTGCAGAAA TCACATTATG GACCTCTGTA TTGAGTGTCA GGCCAATCAG 
GCGTCTGCCA CCTCAGAGGA GTGTACGGTG GCCTGGGGAG TCTGCAATCA CGCTTTTCAT 
TTCCACTGCA TCTCCCGCTG GCTGAAGACC AGACAAGTGT GTCCACTGGA CAACAGAGAG 
TGGGAGTTTC AGAAGTATGG TCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive, individual zebrafish embryos.
BMC genomics18(1)287(2017 04)
Rauwerda H,Pagano JF,de Leeuw WC,Ensink W,Nehrdich U,de Jong M,Jonker M,Spaink HP,Breit TM

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Transcriptional events co-regulated by hypoxia and cold stresses in Zebrafish larvae.
BMC genomics16385(2015 May)
Long Y,Yan J,Song G,Li X,Li X,Li Q,Cui Z