tspan7 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tspan7
Entrez Gene ID	406802
Full Name	tetraspanin 7
Synonyms	tm4sf2,wu:fa11g01,wu:fd18e11,wu:fl39g07,zgc:63823
General protein information	Preferred Names tetraspanin 7 Names tetraspanin-6 tm4sf2a transmembrane 4 superfamily member 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214774.2	NP_999939.1	tetraspanin-6

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tspan7	NP_999939.1
Homo sapiens (human)	TSPAN6	NP_003261.1
Rattus norvegicus (Norway rat)	Tspan6	NP_001094142.1
Gallus gallus (chicken)	TSPAN6	NP_001264532.1
	TSPAN6	XP_001091467.2
Drosophila melanogaster (fruit fly)	Tsp47F	NP_725044.1
Canis lupus familiaris (dog)	TSPAN6	XP_538102.1
Mus musculus (house mouse)	Tspan6	NP_062630.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005233	XP_314136.4
Pan troglodytes (chimpanzee)	TSPAN6	XP_529071.3
Bos taurus (cattle)	TSPAN6	NP_001033198.1
Xenopus tropicalis (tropical clawed frog)	tspan6	NP_001039117.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Tetraspanin 3c requirement for pigment cell interactions and boundary formation in zebrafish adult pigment stripes.
Inoue S, Kondo S, Parichy DM, Watanabe M
Pigment cell & melanoma research27(2)190-200(2014 Mar)

Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets.
Kal?n M, Wallgard E, Asker N, Nasevicius A, Athley E, Billgren E, Larson JD, Wadman SA, Norseng E, Clark KJ, He L, Karlsson-Lindahl L, H?ger AK, Weber H, Augustin H, Samuelsson T, Kemmet CK, Utesch CM, Essner JJ, Hackett PB, Hellstr?m M
Chemistry & biology16(4)432-41(2009 Apr)

The medaka novel immune-type receptor (NITR) gene clusters reveal an extraordinary degree of divergence in variable domains.
Desai S, Heffelfinger AK, Orcutt TM, Litman GW, Yoder JA
BMC evolutionary biology8177(2008 Jun)

Hepatic gene expression profiling using GeneChips in zebrafish exposed to 17alpha-methyldihydrotestosterone.
Hoffmann JL, Thomason RG, Lee DM, Brill JL, Price BB, Carr GJ, Versteeg DJ
Aquatic toxicology (Amsterdam, Netherlands)87(2)69-80(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tspan7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tspan7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98354	NM_214774.2 Latest version!	Danio rerio tetraspanin 7 (tspan7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tspan7 ( NM_214774.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999939.1 Danio rerio tetraspanin 7 (tspan7), mRNA.

Price & Availability↑

CloneID	ODa98354
Clone ID Related Accession (Same CDS sequence)	NM_214774.2
Accession Version	NM_214774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	tetraspanin-6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU459160.5 and BQ262702.1. On Sep 8, 2016 this sequence version replaced NM_214774.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC055255.1, GFIL01042165.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCGCCTC CGTCAGCACG GCTGCAAACC AAACCCGTGA TAACGTGTCT GAAGACGTTT 
CTCATCTCGT ACAGTTTGAT TTTCTGGTTC ACAGGGGTGA TCCTGTTGGC TGTGGGAGTC 
TGGGGAAAGG TTAATTTGGA GTTTGACCTC CTCGTGAATT CAGAACATGG CACCAATGCT 
CCATATGTCC TCATCGGGAC TGGAGCTGTC ATCATCATCT TCGGGCTGTT CGGATGCTTC 
GCCACCTGCC GTGGAAGTCC ATGGATGCTG AAACTGTATG CCATGTTCCT GGTGCTTGTG 
TTCTTAGCTG AACTGGTGGC AGGTATATCC GGATTCATTT TCAGACATGA GATTAAAGCA 
GTGCTTAAGG GTGCCTACCA AAAAGCTGAA TCAAACTACA TGGGTAAAGA TGACGAAGAT 
CTGGACCGGA TCCAGAGAAC ACTGCAATGT TGTGGAGAGG AGAACTACAC AAGCTGGGCG 
AATACAACAT ACTTTGAAAA AGAGGGCATC CCCAAAAGCT GCTGTAACGT GACTGCTAGT 
GTAAACTGCA CTTCTGCAGA ACTCAAGGAT CTCAAAAAAG CTGATTCTGT GGTTTATCAT 
CAGGGCTGTT TCTCTCTGAT GTCCACAACC ATGGAGGCCA ATCTTGGAAT TATTGCTGGA 
ATTTCTTTTG GAATTGCATT CTTCCAGGTC ATTGGGATAT TCTTGGCGTG CTGCCTGTCT 
CGCTATATCA CCAACAACCA ATATGAAATG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999939.1
CDS	163..918
Translation	MSPPSARLQTKPVITCLKTFLISYSLIFWFTGVILLAVGVWGKVNLEFDLLVNSEHGTNAPYVLIGTGAVIIIFGLFGCFATCRGSPWMLKLYAMFLVLVFLAELVAGISGFIFRHEIKAVLKGAYQKAESNYMGKDDEDLDRIQRTLQCCGEENYTSWANTTYFEKEGIPKSCCNVTASVNCTSAELKDLKKADSVVYHQGCFSLMSTTMEANLGIIAGISFGIAFFQVIGIFLACCLSRYITNNQYEMV

Target ORF information:

RefSeq Version	NM_214774.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tetraspanin 7 (tspan7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214774.2

ORF Insert Sequence:

ATGTCGCCTC CGTCAGCACG GCTGCAAACC AAACCCGTGA TAACGTGTCT GAAGACGTTT 
CTCATCTCGT ACAGTTTGAT TTTCTGGTTC ACAGGGGTGA TCCTGTTGGC TGTGGGAGTC 
TGGGGAAAGG TTAATTTGGA GTTTGACCTC CTCGTGAATT CAGAACATGG CACCAATGCT 
CCATATGTCC TCATCGGGAC TGGAGCTGTC ATCATCATCT TCGGGCTGTT CGGATGCTTC 
GCCACCTGCC GTGGAAGTCC ATGGATGCTG AAACTGTATG CCATGTTCCT GGTGCTTGTG 
TTCTTAGCTG AACTGGTGGC AGGTATATCC GGATTCATTT TCAGACATGA GATTAAAGCA 
GTGCTTAAGG GTGCCTACCA AAAAGCTGAA TCAAACTACA TGGGTAAAGA TGACGAAGAT 
CTGGACCGGA TCCAGAGAAC ACTGCAATGT TGTGGAGAGG AGAACTACAC AAGCTGGGCG 
AATACAACAT ACTTTGAAAA AGAGGGCATC CCCAAAAGCT GCTGTAACGT GACTGCTAGT 
GTAAACTGCA CTTCTGCAGA ACTCAAGGAT CTCAAAAAAG CTGATTCTGT GGTTTATCAT 
CAGGGCTGTT TCTCTCTGAT GTCCACAACC ATGGAGGCCA ATCTTGGAAT TATTGCTGGA 
ATTTCTTTTG GAATTGCATT CTTCCAGGTC ATTGGGATAT TCTTGGCGTG CTGCCTGTCT 
CGCTATATCA CCAACAACCA ATATGAAATG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Tetraspanin 3c requirement for pigment cell interactions and boundary formation in zebrafish adult pigment stripes.
Pigment cell & melanoma research27(2)190-200(2014 Mar)
Inoue S,Kondo S,Parichy DM,Watanabe M

Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets.
Chemistry & biology16(4)432-41(2009 Apr)
Kal?n M,Wallgard E,Asker N,Nasevicius A,Athley E,Billgren E,Larson JD,Wadman SA,Norseng E,Clark KJ,He L,Karlsson-Lindahl L,H?ger AK,Weber H,Augustin H,Samuelsson T,Kemmet CK,Utesch CM,Essner JJ,Hackett PB,Hellstr?m M

The medaka novel immune-type receptor (NITR) gene clusters reveal an extraordinary degree of divergence in variable domains.
BMC evolutionary biology8177(2008 Jun)
Desai S,Heffelfinger AK,Orcutt TM,Litman GW,Yoder JA

Hepatic gene expression profiling using GeneChips in zebrafish exposed to 17alpha-methyldihydrotestosterone.
Aquatic toxicology (Amsterdam, Netherlands)87(2)69-80(2008 Apr)
Hoffmann JL,Thomason RG,Lee DM,Brill JL,Price BB,Carr GJ,Versteeg DJ