porcnl cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	porcnl
Entrez Gene ID	333999
Full Name	porcupine O-acyltransferase like
Synonyms	PORCN,wu:fi26a04,zgc:55392
General protein information	Preferred Names porcupine O-acyltransferase like Names porcupine O-acyltransferase like porcn2 porcupine homolog like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199849.1	NP_956143.1	porcupine O-acyltransferase like

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PORCN	XP_005641373.1
Mus musculus (house mouse)	Porcn	NP_665914.1
Rattus norvegicus (Norway rat)	Porcn	NP_001166826.1
Xenopus tropicalis (tropical clawed frog)	porcn	NP_989365.1
Bos taurus (cattle)	PORCN	NP_001094678.1
Danio rerio (zebrafish)	porcnl	NP_956143.1
Anopheles gambiae (African malaria mosquito)	PORCN_ANOGA	XP_317509.1
Homo sapiens (human)	PORCN	NP_982300.1
Pan troglodytes (chimpanzee)	PORCN	XP_521046.2
Danio rerio (zebrafish)	LOC100148644	XP_001923912.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Loss of Porcupine impairs convergent extension during gastrulation in zebrafish.
Chen Q, Takada R, Takada S
Journal of cell science125(Pt 9)2224-34(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of Porcupine impairs convergent extension during gastrulation in zebrafish.
Title: Loss of Porcupine impairs convergent extension during gastrulation in zebrafish.

The following porcnl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the porcnl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33999	NM_199849.1 Latest version!	Danio rerio porcupine O-acyltransferase like (porcnl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

porcnl ( NM_199849.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956143.1 Danio rerio porcupine O-acyltransferase like (porcnl), mRNA.

Price & Availability↑

CloneID	ODa33999
Clone ID Related Accession (Same CDS sequence)	NM_199849.1
Accession Version	NM_199849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-12
Organism	Danio rerio(zebrafish)
Product	porcupine O-acyltransferase like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044156.1. ##Evidence-Data-START## Transcript exon combination :: BC044156.1, BC154563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATTCCT ATGGGCGTAT GGAGTTCCTG CAGGCTTTAG TGGAGGGCTG TGTGTTGCCC 
ACTGCCCTGC AGGGTCTTGA GCAGGTCTGG CAGCTTCTGC TGGTCTGCCT GCTGTCTCGG 
GTCATCTGTA GACTAGGTGT CTCGTGTCAT GTGAAGCATC TGAGCTCGCT GGCAGGCGGT 
CTTTATGTGC TCTACGTGTT TTTCGAGATG CACACGATGT GGGTGATCAT CCTGAGCCTC 
CTCTGCTACC TCATTCTGCT CCTCAGCATC AACTCCAGCA GCCGAGGGGT TTTCCTCTCC 
CTCGTCATCC TCATTTACAT GCTGATGGGG GAACTACACA TGATAGATGC AGCGACCTGG 
CATAAGATGA GAGGGTCCCA GATGGTGGTT GCCATGAAGG CCATCTCTCT AGCCTTTGAC 
CTGGATCGAG GCGCTGTGTC CTCCTTTCCC ACCCCGCTGG AATTCATGGG ATACCTCTTC 
TTTGCAGGAA CAGTCATCTT TGGCCCATGG ATTAGTTTTG CCCGCTACAT AGATGCAATT 
AATGGCCGTA AAATGAGCAC TGCCTGGTTG GGAAAGATTT CTCAGAGCTT TCTGAGTGGT 
CAGATCTGCC TGGTTATTTC CACCTGTATT GCTCCTTATC TCTTTCCTTA CTTTATTCCC 
ATCTACGGAG ACGGACATCC TCGAAGGTGG CTTCACGCTT ATGAAAATGC CTTGTCTTTT 
CACTTTAGCA ATTATTTTGT CAGTTACTTA AGTGAAACAA CAACTGTGAT GGCAGGAGCG 
GGATTCTCTG AACACAAAGA CCACCTCAAA TGGGATATTA CAGTAGCCAA ACCTATGAAT 
GTGGAATTGC CAAGGTCTAT GGTGGAAGTC GTCACGTCCT GGAATCTTCC AATGTCTAAG 
TGGCTCAATG CATATGTTTT TAAAAGCGCA CTCAAACTCG GCACTTTCCC TGCCATCTTG 
GTGACCTACA CAGCCAGTGC GTTGTTGCAT GGTCTCAGCT TTCATCTGGG GGCTGTACTG 
TTGTCTTTGG GATTCATCAC ATATGTTGAA CACGTTCTGA GAAAGCGCTT GGCTGCTATT 
TTCAGTGCTT GTATACTGTC AAAACGCTGC CCAAGTGACT GCCATCACCA AAACAAGAAG 
ACTGTGTGGG TTTATGTTGT AAACCTGTTC TTCAGTGCTC TGTCAATATT CCATTTGACT 
TACTTGGGAT CATTATTTGA CTCTGAAATG GATGAAATGC ACGATGAGGA GGGCTACATG 
GCCAGTCACA CTATTCAGAA GTGGTCGGAG CTAAACTGGG CGAGCCATTG GGTGATGTTA 
GGCTGTTTTG TGTTTTATTG GCTCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956143.1
CDS	64..1413
Translation	MDSYGRMEFLQALVEGCVLPTALQGLEQVWQLLLVCLLSRVICRLGVSCHVKHLSSLAGGLYVLYVFFEMHTMWVIILSLLCYLILLLSINSSSRGVFLSLVILIYMLMGELHMIDAATWHKMRGSQMVVAMKAISLAFDLDRGAVSSFPTPLEFMGYLFFAGTVIFGPWISFARYIDAINGRKMSTAWLGKISQSFLSGQICLVISTCIAPYLFPYFIPIYGDGHPRRWLHAYENALSFHFSNYFVSYLSETTTVMAGAGFSEHKDHLKWDITVAKPMNVELPRSMVEVVTSWNLPMSKWLNAYVFKSALKLGTFPAILVTYTASALLHGLSFHLGAVLLSLGFITYVEHVLRKRLAAIFSACILSKRCPSDCHHQNKKTVWVYVVNLFFSALSIFHLTYLGSLFDSEMDEMHDEEGYMASHTIQKWSELNWASHWVMLGCFVFYWLI

Target ORF information:

RefSeq Version	NM_199849.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio porcupine O-acyltransferase like (porcnl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199849.1

ORF Insert Sequence:

ATGGATTCCT ATGGGCGTAT GGAGTTCCTG CAGGCTTTAG TGGAGGGCTG TGTGTTGCCC 
ACTGCCCTGC AGGGTCTTGA GCAGGTCTGG CAGCTTCTGC TGGTCTGCCT GCTGTCTCGG 
GTCATCTGTA GACTAGGTGT CTCGTGTCAT GTGAAGCATC TGAGCTCGCT GGCAGGCGGT 
CTTTATGTGC TCTACGTGTT TTTCGAGATG CACACGATGT GGGTGATCAT CCTGAGCCTC 
CTCTGCTACC TCATTCTGCT CCTCAGCATC AACTCCAGCA GCCGAGGGGT TTTCCTCTCC 
CTCGTCATCC TCATTTACAT GCTGATGGGG GAACTACACA TGATAGATGC AGCGACCTGG 
CATAAGATGA GAGGGTCCCA GATGGTGGTT GCCATGAAGG CCATCTCTCT AGCCTTTGAC 
CTGGATCGAG GCGCTGTGTC CTCCTTTCCC ACCCCGCTGG AATTCATGGG ATACCTCTTC 
TTTGCAGGAA CAGTCATCTT TGGCCCATGG ATTAGTTTTG CCCGCTACAT AGATGCAATT 
AATGGCCGTA AAATGAGCAC TGCCTGGTTG GGAAAGATTT CTCAGAGCTT TCTGAGTGGT 
CAGATCTGCC TGGTTATTTC CACCTGTATT GCTCCTTATC TCTTTCCTTA CTTTATTCCC 
ATCTACGGAG ACGGACATCC TCGAAGGTGG CTTCACGCTT ATGAAAATGC CTTGTCTTTT 
CACTTTAGCA ATTATTTTGT CAGTTACTTA AGTGAAACAA CAACTGTGAT GGCAGGAGCG 
GGATTCTCTG AACACAAAGA CCACCTCAAA TGGGATATTA CAGTAGCCAA ACCTATGAAT 
GTGGAATTGC CAAGGTCTAT GGTGGAAGTC GTCACGTCCT GGAATCTTCC AATGTCTAAG 
TGGCTCAATG CATATGTTTT TAAAAGCGCA CTCAAACTCG GCACTTTCCC TGCCATCTTG 
GTGACCTACA CAGCCAGTGC GTTGTTGCAT GGTCTCAGCT TTCATCTGGG GGCTGTACTG 
TTGTCTTTGG GATTCATCAC ATATGTTGAA CACGTTCTGA GAAAGCGCTT GGCTGCTATT 
TTCAGTGCTT GTATACTGTC AAAACGCTGC CCAAGTGACT GCCATCACCA AAACAAGAAG 
ACTGTGTGGG TTTATGTTGT AAACCTGTTC TTCAGTGCTC TGTCAATATT CCATTTGACT 
TACTTGGGAT CATTATTTGA CTCTGAAATG GATGAAATGC ACGATGAGGA GGGCTACATG 
GCCAGTCACA CTATTCAGAA GTGGTCGGAG CTAAACTGGG CGAGCCATTG GGTGATGTTA 
GGCTGTTTTG TGTTTTATTG GCTCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Loss of Porcupine impairs convergent extension during gastrulation in zebrafish.
Journal of cell science125(Pt 9)2224-34(2012 May)
Chen Q,Takada R,Takada S