gas8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gas8
Entrez Gene ID	323260
Full Name	growth arrest-specific 8
Synonyms	wu:fb93e12,zgc:66271
General protein information	Preferred Names growth arrest-specific 8 Names growth arrest-specific protein 8
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199634.1	NP_955928.1	dynein regulatory complex subunit 4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	gas8	NP_955928.1
Drosophila melanogaster (fruit fly)	Gas8	NP_570065.2
Homo sapiens (human)	GAS8	NP_001472.1
Canis lupus familiaris (dog)	GAS8	XP_005620585.1
Mus musculus (house mouse)	Gas8	NP_061343.2
Gallus gallus (chicken)	GAS8	NP_001264546.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005850	XP_001237728.2
Pan troglodytes (chimpanzee)	GAS8	XP_511182.2
Xenopus tropicalis (tropical clawed frog)	gas8	NP_001106441.1
	GAS8	XP_001093935.1
Bos taurus (cattle)	GAS8	NP_001091038.1
Rattus norvegicus (Norway rat)	Gas8	NP_001034119.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
Thomas HR, Percival SM, Yoder BK, Parant JM
PloS one9(12)e114632(2014)

Characterization of Oncorhynchus mykiss 5-hydroxyisourate hydrolase/transthyretin superfamily: evolutionary and functional analyses.
Kasai K, Nishiyama N, Yamauchi K
Gene531(2)326-36(2013 Dec)

Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling.
Evron T, Philipp M, Lu J, Meloni AR, Burkhalter M, Chen W, Caron MG
The Journal of biological chemistry286(31)27676-86(2011 Aug)

The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear.
Colantonio JR, Vermot J, Wu D, Langenbacher AD, Fraser S, Chen JN, Hill KL
Nature457(7226)205-9(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate a requirement for the dynein regulatory complex in vertebrates and show that cilia-driven flow is a key epigenetic factor in controlling otolith biomineralization
Title: The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear.

The following gas8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gas8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34917	NM_199634.1 Latest version!	Danio rerio growth arrest-specific 8 (gas8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gas8 ( NM_199634.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955928.1 Danio rerio growth arrest-specific 8 (gas8), mRNA.

Price & Availability↑

CloneID	ODa34917
Clone ID Related Accession (Same CDS sequence)	NM_199634.1
Accession Version	NM_199634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-07
Organism	Danio rerio(zebrafish)
Product	dynein regulatory complex subunit 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057501.1. ##Evidence-Data-START## Transcript exon combination :: BC057501.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCACCAA AAAGAAAATC GTCGCCGAAG GGCAAAACTC CAACAGTAGT GGACGGCTTG 
TCCACAGAGG AGATGTCAAA AGAGCAGTTG GAGGAGCACA TAATGCGTCT CCGAGAGGAG 
CTGGACAGGG AGAGAGAAGA ACGCAACTAT TTCCAGCTAG AGAGGGACAA AATTCACACT 
TTCTGGGAAA TTACACGACG ACATCTCGAA GAGAATAAGT GTGAGCTGAG AAACAGAGAG 
CGTGAATTGG AGGAAGTAGA GGAACGCCAC CAAGTGGAAA TTAAGGTTTA TAAACAGAAG 
GTGAAGCACT TGCTGTACGA ACAGCAGAAC ATGCTGTCTG AGCTAAAGGC TGAAAGCATC 
ATCAGCACCA AACTGCTGCA GGATGAGCAT GCTGATTTGG AGAAGGAGCA GTGGAAGGAT 
ATGCGCAGTT TGAAACTTGA GTTGAAACAA CAAGAGCTGT CCAGTGAAAA TGTGTTAAAG 
AGAATTCATC TAAAACATGA TGAGGAAACT ACTGATTTGA GAAATGATTT TGCCAGACAA 
GTACAAGAAA TTGGGTCCAA ATATGAGAAA AGGATGCAGA AACTGCGTCA GGAGGAAGAA 
CTGAGACGCA AGACAGAGAT TCATGAGATT GAGGAGCGGA AGAACACCCA CATTAACATG 
CTGATGAAGA ACCATGAAAA AGCTTACAGA GACATCAGAA ACTACTTTAG TGACATAGTC 
TACAAGAATC TGGACCTTAT CACTTCACTC AAGGAAGAGT TAAAGGAAAT GAAAAAGAAC 
GAGGAGAAGA GAAATAAAGA GATGGCAGAA GTACTTGAGC AGAATAAGGA GCTTAAAGAG 
TCTTCACAGA AGGCAAAAGA ACAAGTTGCT GAGCTTCAGA AACAGCTTGC CAATTATGAG 
AAGGACAAGA GCATTTTAAC TCGGTCAAGA GCTCGTCTCA AAATGTCTGA CAGAGAAATG 
AAAGAGCTTA AGTGGGAGCT TGAAGTGTTG GAGCAGAGAT TTTCTAAGGT TCAACTGGAG 
CGGGACGAAT TGTACATGAA GTTCACTAAA GCCATTCTAG AGGTTCAACA GAAGAGTGGT 
TTTAAAAACT TGCTGCTTGA GTGCAAATTG AACACACTCA ATGACACGCT TGAGAAGAAG 
GAAGCCCAGC TCAGTGAGGT GCTGTCTGCT TCCAACCTGG ACCCCACCAC CCTCAGTGTG 
GTCACCCATA AATTGGAGGA AGTTTTGGAG TCCAAAAACC ATACTATTAA AGACTTACAG 
TATGAAGTTG CACGAGTTTG CAAGGCCCAT AATGACTTGC TAAAGACTTC TGAAGCAAAG 
CTGCGAGCAT TTGGTATTCC TGTGGAAGAG CTCTGCTTCG AGCCACTTAA AAGCAATGGA 
CAGAGTGTTG GCCAGGGCCC TGCTATGTTT GTTTCTTCTT CAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955928.1
CDS	93..1520
Translation	MPPKRKSSPKGKTPTVVDGLSTEEMSKEQLEEHIMRLREELDREREERNYFQLERDKIHTFWEITRRHLEENKCELRNRERELEEVEERHQVEIKVYKQKVKHLLYEQQNMLSELKAESIISTKLLQDEHADLEKEQWKDMRSLKLELKQQELSSENVLKRIHLKHDEETTDLRNDFARQVQEIGSKYEKRMQKLRQEEELRRKTEIHEIEERKNTHINMLMKNHEKAYRDIRNYFSDIVYKNLDLITSLKEELKEMKKNEEKRNKEMAEVLEQNKELKESSQKAKEQVAELQKQLANYEKDKSILTRSRARLKMSDREMKELKWELEVLEQRFSKVQLERDELYMKFTKAILEVQQKSGFKNLLLECKLNTLNDTLEKKEAQLSEVLSASNLDPTTLSVVTHKLEEVLESKNHTIKDLQYEVARVCKAHNDLLKTSEAKLRAFGIPVEELCFEPLKSNGQSVGQGPAMFVSSSN

Target ORF information:

RefSeq Version	NM_199634.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio growth arrest-specific 8 (gas8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199634.1

ORF Insert Sequence:

ATGCCACCAA AAAGAAAATC GTCGCCGAAG GGCAAAACTC CAACAGTAGT GGACGGCTTG 
TCCACAGAGG AGATGTCAAA AGAGCAGTTG GAGGAGCACA TAATGCGTCT CCGAGAGGAG 
CTGGACAGGG AGAGAGAAGA ACGCAACTAT TTCCAGCTAG AGAGGGACAA AATTCACACT 
TTCTGGGAAA TTACACGACG ACATCTCGAA GAGAATAAGT GTGAGCTGAG AAACAGAGAG 
CGTGAATTGG AGGAAGTAGA GGAACGCCAC CAAGTGGAAA TTAAGGTTTA TAAACAGAAG 
GTGAAGCACT TGCTGTACGA ACAGCAGAAC ATGCTGTCTG AGCTAAAGGC TGAAAGCATC 
ATCAGCACCA AACTGCTGCA GGATGAGCAT GCTGATTTGG AGAAGGAGCA GTGGAAGGAT 
ATGCGCAGTT TGAAACTTGA GTTGAAACAA CAAGAGCTGT CCAGTGAAAA TGTGTTAAAG 
AGAATTCATC TAAAACATGA TGAGGAAACT ACTGATTTGA GAAATGATTT TGCCAGACAA 
GTACAAGAAA TTGGGTCCAA ATATGAGAAA AGGATGCAGA AACTGCGTCA GGAGGAAGAA 
CTGAGACGCA AGACAGAGAT TCATGAGATT GAGGAGCGGA AGAACACCCA CATTAACATG 
CTGATGAAGA ACCATGAAAA AGCTTACAGA GACATCAGAA ACTACTTTAG TGACATAGTC 
TACAAGAATC TGGACCTTAT CACTTCACTC AAGGAAGAGT TAAAGGAAAT GAAAAAGAAC 
GAGGAGAAGA GAAATAAAGA GATGGCAGAA GTACTTGAGC AGAATAAGGA GCTTAAAGAG 
TCTTCACAGA AGGCAAAAGA ACAAGTTGCT GAGCTTCAGA AACAGCTTGC CAATTATGAG 
AAGGACAAGA GCATTTTAAC TCGGTCAAGA GCTCGTCTCA AAATGTCTGA CAGAGAAATG 
AAAGAGCTTA AGTGGGAGCT TGAAGTGTTG GAGCAGAGAT TTTCTAAGGT TCAACTGGAG 
CGGGACGAAT TGTACATGAA GTTCACTAAA GCCATTCTAG AGGTTCAACA GAAGAGTGGT 
TTTAAAAACT TGCTGCTTGA GTGCAAATTG AACACACTCA ATGACACGCT TGAGAAGAAG 
GAAGCCCAGC TCAGTGAGGT GCTGTCTGCT TCCAACCTGG ACCCCACCAC CCTCAGTGTG 
GTCACCCATA AATTGGAGGA AGTTTTGGAG TCCAAAAACC ATACTATTAA AGACTTACAG 
TATGAAGTTG CACGAGTTTG CAAGGCCCAT AATGACTTGC TAAAGACTTC TGAAGCAAAG 
CTGCGAGCAT TTGGTATTCC TGTGGAAGAG CTCTGCTTCG AGCCACTTAA AAGCAATGGA 
CAGAGTGTTG GCCAGGGCCC TGCTATGTTT GTTTCTTCTT CAAATTAG