psme1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	psme1
Entrez Gene ID	30648
Full Name	proteasome activator subunit 1
Synonyms	fk89d05,wu:fk89d05
General protein information	Preferred Names proteasome activator subunit 1 Names proteasome activator complex subunit 1 pA23-a proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131375.2	NP_571450.1	proteasome activator complex subunit 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSME1	NP_006254.1
	PSME1	XP_001104073.1
Canis lupus familiaris (dog)	PSME1	XP_537380.1
Bos taurus (cattle)	PSME1	NP_001019680.2
Pan troglodytes (chimpanzee)	PSME1	XP_509864.3
Danio rerio (zebrafish)	psme1	NP_571450.1
Mus musculus (house mouse)	Psme1	NP_035319.1
Rattus norvegicus (Norway rat)	Psme1	NP_058960.2
Xenopus tropicalis (tropical clawed frog)	psme1	NP_001011302.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss of DNA methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling.
Chernyavskaya Y, Mudbhary R, Zhang C, Tokarz D, Jacob V, Gopinath S, Sun X, Wang S, Magnani E, Madakashira BP, Yoder JA, Hoshida Y, Sadler KC
Development (Cambridge, England)144(16)2925-2939(2017 08)

Transcriptome Analysis Reveals Increases in Visceral Lipogenesis and Storage and Activation of the Antigen Processing and Presentation Pathway during the Mouth-Opening Stage in Zebrafish Larvae.
Xu H, Liu E, Li Y, Li X, Ding C
International journal of molecular sciences18(8)(2017 Jul)

Unexpected role of interferon-? in regulating neuronal connectivity and social behaviour.
Filiano AJ, Xu Y, Tustison NJ, Marsh RL, Baker W, Smirnov I, Overall CC, Gadani SP, Turner SD, Weng Z, Peerzade SN, Chen H, Lee KS, Scott MM, Beenhakker MP, Litvak V, Kipnis J
Nature535(7612)425-9(2016 07)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.
Yang D, Liu Q, Yang M, Wu H, Wang Q, Xiao J, Zhang Y
BMC genomics13319(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following psme1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the psme1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03638	NM_131375.2 Latest version!	Danio rerio proteasome activator subunit 1 (psme1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

psme1 ( NM_131375.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571450.1 Danio rerio proteasome activator subunit 1 (psme1), mRNA.

Price & Availability↑

CloneID	ODa03638
Clone ID Related Accession (Same CDS sequence)	NM_131375.2
Accession Version	NM_131375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	proteasome activator complex subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF195052.1. On Aug 31, 2012 this sequence version replaced NM_131375.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF195052.1, BC075864.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACTTCCT TAGACATGAG CCCCGCGTCC AAGAAACAGG TGGATGGATT CTCTCAGAAA 
ATCACCAAGG AGGCTGAACA GTTGATCTCG AAGATTTTCC CTGAGAAGAT TGCAGAGATG 
GACAATGTGT TGCAGGGTTC CTGTAGTCTG AAGGATCTTT CTGTGATCAA GGCTCCTCTG 
GACATCCCAA TTCCAGACCC GGTTAAGGAG GAGCTCAAGA GGAAGAAAAA AGAGGAGAAA 
GAGGCCAAAG AGGGAAAGAA AGATAAAGAA GATGAGGATG CAGGTCCTCC ATGCGGTCCT 
ATCGCCTGCA ACGAGACAGT AGAGAAACTC ATCAAACAGA TCAAGCCTGA GATTCAAACT 
TTGAAAGAGT GTCTTAACAC GGTGTCAATG TGGATACAGC TACAGGTACC CAGAATTGAA 
GATGGGAACA ACTTTGGTGT TGCTGTACAG GAAAAGGTTT TTGAACTGAT GACCAACACC 
CGCACCAAGA TCGAAGGATT CCAGACACAG ATATCCAAGT ACTACAGTGA GAGAGGAGAC 
GCTGTAGCCA AGGCTTCCAA ACAGCCACAT GTGGGAGATT TCCGACAGCT TGTTCATGAA 
CTGGATCAGC ACCAGTATTG TGAGTTACGC ATCATAGTTC TGGAGATTCG CAACACTTAC 
GCCATGCTGT ATGATGTCAT CACCAAGAAC TTTGACAAGA TTAAGAAGCC CAGGGGAGAC 
TTATCTTCAA AAGCACTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571450.1
CDS	151..897
Translation	MTSLDMSPASKKQVDGFSQKITKEAEQLISKIFPEKIAEMDNVLQGSCSLKDLSVIKAPLDIPIPDPVKEELKRKKKEEKEAKEGKKDKEDEDAGPPCGPIACNETVEKLIKQIKPEIQTLKECLNTVSMWIQLQVPRIEDGNNFGVAVQEKVFELMTNTRTKIEGFQTQISKYYSERGDAVAKASKQPHVGDFRQLVHELDQHQYCELRIIVLEIRNTYAMLYDVITKNFDKIKKPRGDLSSKALIY

Target ORF information:

RefSeq Version	NM_131375.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proteasome activator subunit 1 (psme1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131375.2

ORF Insert Sequence:

ATGACTTCCT TAGACATGAG CCCCGCGTCC AAGAAACAGG TGGATGGATT CTCTCAGAAA 
ATCACCAAGG AGGCTGAACA GTTGATCTCG AAGATTTTCC CTGAGAAGAT TGCAGAGATG 
GACAATGTGT TGCAGGGTTC CTGTAGTCTG AAGGATCTTT CTGTGATCAA GGCTCCTCTG 
GACATCCCAA TTCCAGACCC GGTTAAGGAG GAGCTCAAGA GGAAGAAAAA AGAGGAGAAA 
GAGGCCAAAG AGGGAAAGAA AGATAAAGAA GATGAGGATG CAGGTCCTCC ATGCGGTCCT 
ATCGCCTGCA ACGAGACAGT AGAGAAACTC ATCAAACAGA TCAAGCCTGA GATTCAAACT 
TTGAAAGAGT GTCTTAACAC GGTGTCAATG TGGATACAGC TACAGGTACC CAGAATTGAA 
GATGGGAACA ACTTTGGTGT TGCTGTACAG GAAAAGGTTT TTGAACTGAT GACCAACACC 
CGCACCAAGA TCGAAGGATT CCAGACACAG ATATCCAAGT ACTACAGTGA GAGAGGAGAC 
GCTGTAGCCA AGGCTTCCAA ACAGCCACAT GTGGGAGATT TCCGACAGCT TGTTCATGAA 
CTGGATCAGC ACCAGTATTG TGAGTTACGC ATCATAGTTC TGGAGATTCG CAACACTTAC 
GCCATGCTGT ATGATGTCAT CACCAAGAAC TTTGACAAGA TTAAGAAGCC CAGGGGAGAC 
TTATCTTCAA AAGCACTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of DNA methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling.
Development (Cambridge, England)144(16)2925-2939(2017 08)
Chernyavskaya Y,Mudbhary R,Zhang C,Tokarz D,Jacob V,Gopinath S,Sun X,Wang S,Magnani E,Madakashira BP,Yoder JA,Hoshida Y,Sadler KC

Transcriptome Analysis Reveals Increases in Visceral Lipogenesis and Storage and Activation of the Antigen Processing and Presentation Pathway during the Mouth-Opening Stage in Zebrafish Larvae.
International journal of molecular sciences18(8)(2017 Jul)
Xu H,Liu E,Li Y,Li X,Ding C

Unexpected role of interferon-? in regulating neuronal connectivity and social behaviour.
Nature535(7612)425-9(2016 07)
Filiano AJ,Xu Y,Tustison NJ,Marsh RL,Baker W,Smirnov I,Overall CC,Gadani SP,Turner SD,Weng Z,Peerzade SN,Chen H,Lee KS,Scott MM,Beenhakker MP,Litvak V,Kipnis J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.
BMC genomics13319(2012 Jul)
Yang D,Liu Q,Yang M,Wu H,Wang Q,Xiao J,Zhang Y