hoxd3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hoxd3a
Entrez Gene ID	30349
Full Name	homeobox D3a
Synonyms	Z-56,hoxd3,im:7144208
General protein information	Preferred Names homeobox D3a Names homeobox protein Hox-D3a homeo box D3a homeobox gene D-3 hox-D3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131125.1	NP_571200.1	homeobox protein Hox-D3a
XM_021478602.1	XP_021334277.1	homeobox protein Hox-D3a isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HOXD3	NP_008829.3
Gallus gallus (chicken)	HOXD3	XP_428765.2
Danio rerio (zebrafish)	hoxd3a	NP_571200.1
Mus musculus (house mouse)	Hoxd3	NP_034598.2
Xenopus tropicalis (tropical clawed frog)	hoxd3	XP_004917786.1
Canis lupus familiaris (dog)	HOXD3	XP_545539.2
Bos taurus (cattle)	HOXD3	XP_005202439.1
Rattus norvegicus (Norway rat)	Hoxd3	NP_001257967.1
Pan troglodytes (chimpanzee)	HOXD3	XP_001153177.1

Gene Ontology
Bibliography

Related articles in PubMed

Variant Histone H2afv reprograms DNA methylation during early zebrafish development.
Madakashira B, Corbett L, Zhang C, Paoli P, Casement JW, Mann J, Sadler KC, Mann DA
Epigenetics12(9)811-824(2017 Sep)

Tbx16 regulates hox gene activation in mesodermal progenitor cells.
Payumo AY, McQuade LE, Walker WJ, Yamazoe S, Chen JK
Nature chemical biology12(9)694-701(2016 09)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Time point-based integrative analyses of deep-transcriptome identify four signal pathways in blastemal regeneration of zebrafish lower jaw.
Zhang H, Wang X, Lyu K, Gao S, Wang G, Fan C, Zhang XA, Yan J
Stem cells (Dayton, Ohio)33(3)806-18(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Oculomotor circuits in hindbrain rhombomeres 5-6 develop and function independently of hox3 genes.
Title: Development of oculomotor circuitry independent of hox3 genes.

hoxd3 gene is mainly expressed in nervous system of wide-type zebrafish embryos.
Title: [Expression pattern of hoxd3 gene during early development of wild-type zebrafish embryos].

The following hoxd3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hoxd3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02885	NM_131125.1 Latest version!	Danio rerio homeobox D3a (hoxd3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa02885	XM_021478602.1 Latest version!	Danio rerio homeobox D3a (hoxd3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hoxd3a ( NM_131125.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571200.1 Danio rerio homeobox D3a (hoxd3a), mRNA.

Price & Availability↑

CloneID	ODa02885
Clone ID Related Accession (Same CDS sequence)	NM_131125.1 , XM_021478602.1
Accession Version	NM_131125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	homeobox protein Hox-D3a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX322661.17. On Mar 22, 2009 this sequence version replaced XM_001922716.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EL647436.1, DQ060564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAAAG CCACTTATTA TGACAATGCT GGACTTTTCG GAGGATATTC GTACCCTAAA 
TCTGACTCCT ACACGTACGG TCCAACACAC CAGGGCTTCT CATCCTCCAG TATAGAGAAT 
GACTATCAAA GCCCAATTTG TCCAATTCAA ACTACATCAG TCCGGCAAGC AACCCATAAA 
AACGGAGACA TCAATGGCAG TTGTATGCGA CCAAGTGCCA GTCAGGGCAA CAGTCAGCCA 
GAGAGTATCA GTGAGCAGCA GCAGGCTGCT CCATTAGCGG CCAGCTCTCC CAGCCCCAGC 
ACTAACTCCA CACAAAAAAA GAAATCCCCC AGCTCTAATG GATCCAGCAC TGCCACCCCA 
GTCATCTCCA AGCAAATTTT CCCCTGGATG AAAGAGACCC GCCAAAATGC AAAGCAGAAA 
AGCACCAACT GCCCAGCAGC AGGAGAAACC TGCGATGACA AGAGTCCGCC TGGCCCTGCG 
TCGAAACGAG TGCGCACTGC CTACACCAGC GCCCAGCTAG TCGAGCTGGA GAAGGAGTTT 
CATTTCAACC GCTACCTGTG TCGCCCCAGA AGAGTGGAGA TGGCCAATCT GCTCAACCTC 
ACCGAGCGCC AAATAAAGAT CTGGTTTCAA AACAGAAGGA TGAAGTACAA AAAAGATCAG 
AAATCTAAAG GCATAATGCA CTCTCCCTTA GGACACTCTC CGGACAGGAG CCCGCCGTTA 
AGTGGCTCGA ATCACATTGG ATATTCTGGT CAGCTTCCGA ACGTAAACAG CCTCAGCTAC 
GACGCGCCGT CGCCTCCGTC ATTCGCCAAG CCGCAGCAGA ATATATACGG CTTGGCGGCA 
TACACGGCGC CATTGGGCGG CTGCATACCG CAGCAGAAAA GGTACCCGGG AACAGAGTAC 
GACCACCACA GCATGCAGGG GAACGGTAGC TTTGCTAATG CTAATTTACA AAGTAGCCCC 
GTGTACGTCG GGGGAAACTT CGTTGATTCA ATGCCAGCCT CCGGCCCCAT GTTCAACCTC 
GGTCATCTTC CCCATCCCTC ATCCGCTAGT GTGGACTACA GCTGTGCCGC TCAGATTCCG 
GGTAACCACC ACCACGGACC GTGTGACCCC CACCCCACAT ACACAGACCT CAGCTCTCAT 
CATGCGTCTC AGGGAAGGCT GCAGGATGCG CCCAAACTCA CGCACCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571200.1
CDS	1..1191
Translation	MQKATYYDNAGLFGGYSYPKSDSYTYGPTHQGFSSSSIENDYQSPICPIQTTSVRQATHKNGDINGSCMRPSASQGNSQPESISEQQQAAPLAASSPSPSTNSTQKKKSPSSNGSSTATPVISKQIFPWMKETRQNAKQKSTNCPAAGETCDDKSPPGPASKRVRTAYTSAQLVELEKEFHFNRYLCRPRRVEMANLLNLTERQIKIWFQNRRMKYKKDQKSKGIMHSPLGHSPDRSPPLSGSNHIGYSGQLPNVNSLSYDAPSPPSFAKPQQNIYGLAAYTAPLGGCIPQQKRYPGTEYDHHSMQGNGSFANANLQSSPVYVGGNFVDSMPASGPMFNLGHLPHPSSASVDYSCAAQIPGNHHHGPCDPHPTYTDLSSHHASQGRLQDAPKLTHL

Target ORF information:

RefSeq Version	NM_131125.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio homeobox D3a (hoxd3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131125.1

ORF Insert Sequence:

ATGCAGAAAG CCACTTATTA TGACAATGCT GGACTTTTCG GAGGATATTC GTACCCTAAA 
TCTGACTCCT ACACGTACGG TCCAACACAC CAGGGCTTCT CATCCTCCAG TATAGAGAAT 
GACTATCAAA GCCCAATTTG TCCAATTCAA ACTACATCAG TCCGGCAAGC AACCCATAAA 
AACGGAGACA TCAATGGCAG TTGTATGCGA CCAAGTGCCA GTCAGGGCAA CAGTCAGCCA 
GAGAGTATCA GTGAGCAGCA GCAGGCTGCT CCATTAGCGG CCAGCTCTCC CAGCCCCAGC 
ACTAACTCCA CACAAAAAAA GAAATCCCCC AGCTCTAATG GATCCAGCAC TGCCACCCCA 
GTCATCTCCA AGCAAATTTT CCCCTGGATG AAAGAGACCC GCCAAAATGC AAAGCAGAAA 
AGCACCAACT GCCCAGCAGC AGGAGAAACC TGCGATGACA AGAGTCCGCC TGGCCCTGCG 
TCGAAACGAG TGCGCACTGC CTACACCAGC GCCCAGCTAG TCGAGCTGGA GAAGGAGTTT 
CATTTCAACC GCTACCTGTG TCGCCCCAGA AGAGTGGAGA TGGCCAATCT GCTCAACCTC 
ACCGAGCGCC AAATAAAGAT CTGGTTTCAA AACAGAAGGA TGAAGTACAA AAAAGATCAG 
AAATCTAAAG GCATAATGCA CTCTCCCTTA GGACACTCTC CGGACAGGAG CCCGCCGTTA 
AGTGGCTCGA ATCACATTGG ATATTCTGGT CAGCTTCCGA ACGTAAACAG CCTCAGCTAC 
GACGCGCCGT CGCCTCCGTC ATTCGCCAAG CCGCAGCAGA ATATATACGG CTTGGCGGCA 
TACACGGCGC CATTGGGCGG CTGCATACCG CAGCAGAAAA GGTACCCGGG AACAGAGTAC 
GACCACCACA GCATGCAGGG GAACGGTAGC TTTGCTAATG CTAATTTACA AAGTAGCCCC 
GTGTACGTCG GGGGAAACTT CGTTGATTCA ATGCCAGCCT CCGGCCCCAT GTTCAACCTC 
GGTCATCTTC CCCATCCCTC ATCCGCTAGT GTGGACTACA GCTGTGCCGC TCAGATTCCG 
GGTAACCACC ACCACGGACC GTGTGACCCC CACCCCACAT ACACAGACCT CAGCTCTCAT 
CATGCGTCTC AGGGAAGGCT GCAGGATGCG CCCAAACTCA CGCACCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hoxd3a ( XM_021478602.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334277.1 Danio rerio homeobox D3a (hoxd3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa02885
Clone ID Related Accession (Same CDS sequence)	NM_131125.1 , XM_021478602.1
Accession Version	XM_021478602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	homeobox protein Hox-D3a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCACTTATTA TGACAATGCT GGACTTTTCG GAGGATATTC GTACCCTAAA 
TCTGACTCCT ACACGTACGG TCCAACACAC CAGGGCTTCT CATCCTCCAG TATAGAGAAT 
GACTATCAAA GCCCAATTTG TCCAATTCAA ACTACATCAG TCCGGCAAGC AACCCATAAA 
AACGGAGACA TCAATGGCAG TTGTATGCGA CCAAGTGCCA GTCAGGGCAA CAGTCAGCCA 
GAGAGTATCA GTGAGCAGCA GCAGGCTGCT CCATTAGCGG CCAGCTCTCC CAGCCCCAGC 
ACTAACTCCA CACAAAAAAA GAAATCCCCC AGCTCTAATG GATCCAGCAC TGCCACCCCA 
GTCATCTCCA AGCAAATTTT CCCCTGGATG AAAGAGACCC GCCAAAATGC AAAGCAGAAA 
AGCACCAACT GCCCAGCAGC AGGAGAAACC TGCGATGACA AGAGTCCGCC TGGCCCTGCG 
TCGAAACGAG TGCGCACTGC CTACACCAGC GCCCAGCTAG TCGAGCTGGA GAAGGAGTTT 
CATTTCAACC GCTACCTGTG TCGCCCCAGA AGAGTGGAGA TGGCCAATCT GCTCAACCTC 
ACCGAGCGCC AAATAAAGAT CTGGTTTCAA AACAGAAGGA TGAAGTACAA AAAAGATCAG 
AAATCTAAAG GCATAATGCA CTCTCCCTTA GGACACTCTC CGGACAGGAG CCCGCCGTTA 
AGTGGCTCGA ATCACATTGG ATATTCTGGT CAGCTTCCGA ACGTAAACAG CCTCAGCTAC 
GACGCGCCGT CGCCTCCGTC ATTCGCCAAG CCGCAGCAGA ATATATACGG CTTGGCGGCA 
TACACGGCGC CATTGGGCGG CTGCATACCG CAGCAGAAAA GGTACCCGGG AACAGAGTAC 
GACCACCACA GCATGCAGGG GAACGGTAGC TTTGCTAATG CTAATTTACA AAGTAGCCCC 
GTGTACGTCG GGGGAAACTT CGTTGATTCA ATGCCAGCCT CCGGCCCCAT GTTCAACCTC 
GGTCATCTTC CCCATCCCTC ATCCGCTAGT GTGGACTACA GCTGTGCCGC TCAGATTCCG 
GGTAACCACC ACCACGGACC GTGTGACCCC CACCCCACAT ACACAGACCT CAGCTCTCAT 
CATGCGTCTC AGGGAAGGCT GCAGGATGCG CCCAAACTCA CGCACCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334277.1
CDS	627..1817
Translation	MQKATYYDNAGLFGGYSYPKSDSYTYGPTHQGFSSSSIENDYQSPICPIQTTSVRQATHKNGDINGSCMRPSASQGNSQPESISEQQQAAPLAASSPSPSTNSTQKKKSPSSNGSSTATPVISKQIFPWMKETRQNAKQKSTNCPAAGETCDDKSPPGPASKRVRTAYTSAQLVELEKEFHFNRYLCRPRRVEMANLLNLTERQIKIWFQNRRMKYKKDQKSKGIMHSPLGHSPDRSPPLSGSNHIGYSGQLPNVNSLSYDAPSPPSFAKPQQNIYGLAAYTAPLGGCIPQQKRYPGTEYDHHSMQGNGSFANANLQSSPVYVGGNFVDSMPASGPMFNLGHLPHPSSASVDYSCAAQIPGNHHHGPCDPHPTYTDLSSHHASQGRLQDAPKLTHL

Target ORF information:

RefSeq Version	XM_021478602.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio homeobox D3a (hoxd3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478602.1

ORF Insert Sequence:

ATGCAGAAAG CCACTTATTA TGACAATGCT GGACTTTTCG GAGGATATTC GTACCCTAAA 
TCTGACTCCT ACACGTACGG TCCAACACAC CAGGGCTTCT CATCCTCCAG TATAGAGAAT 
GACTATCAAA GCCCAATTTG TCCAATTCAA ACTACATCAG TCCGGCAAGC AACCCATAAA 
AACGGAGACA TCAATGGCAG TTGTATGCGA CCAAGTGCCA GTCAGGGCAA CAGTCAGCCA 
GAGAGTATCA GTGAGCAGCA GCAGGCTGCT CCATTAGCGG CCAGCTCTCC CAGCCCCAGC 
ACTAACTCCA CACAAAAAAA GAAATCCCCC AGCTCTAATG GATCCAGCAC TGCCACCCCA 
GTCATCTCCA AGCAAATTTT CCCCTGGATG AAAGAGACCC GCCAAAATGC AAAGCAGAAA 
AGCACCAACT GCCCAGCAGC AGGAGAAACC TGCGATGACA AGAGTCCGCC TGGCCCTGCG 
TCGAAACGAG TGCGCACTGC CTACACCAGC GCCCAGCTAG TCGAGCTGGA GAAGGAGTTT 
CATTTCAACC GCTACCTGTG TCGCCCCAGA AGAGTGGAGA TGGCCAATCT GCTCAACCTC 
ACCGAGCGCC AAATAAAGAT CTGGTTTCAA AACAGAAGGA TGAAGTACAA AAAAGATCAG 
AAATCTAAAG GCATAATGCA CTCTCCCTTA GGACACTCTC CGGACAGGAG CCCGCCGTTA 
AGTGGCTCGA ATCACATTGG ATATTCTGGT CAGCTTCCGA ACGTAAACAG CCTCAGCTAC 
GACGCGCCGT CGCCTCCGTC ATTCGCCAAG CCGCAGCAGA ATATATACGG CTTGGCGGCA 
TACACGGCGC CATTGGGCGG CTGCATACCG CAGCAGAAAA GGTACCCGGG AACAGAGTAC 
GACCACCACA GCATGCAGGG GAACGGTAGC TTTGCTAATG CTAATTTACA AAGTAGCCCC 
GTGTACGTCG GGGGAAACTT CGTTGATTCA ATGCCAGCCT CCGGCCCCAT GTTCAACCTC 
GGTCATCTTC CCCATCCCTC ATCCGCTAGT GTGGACTACA GCTGTGCCGC TCAGATTCCG 
GGTAACCACC ACCACGGACC GTGTGACCCC CACCCCACAT ACACAGACCT CAGCTCTCAT 
CATGCGTCTC AGGGAAGGCT GCAGGATGCG CCCAAACTCA CGCACCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variant Histone H2afv reprograms DNA methylation during early zebrafish development.
Epigenetics12(9)811-824(2017 Sep)
Madakashira B,Corbett L,Zhang C,Paoli P,Casement JW,Mann J,Sadler KC,Mann DA

Tbx16 regulates hox gene activation in mesodermal progenitor cells.
Nature chemical biology12(9)694-701(2016 09)
Payumo AY,McQuade LE,Walker WJ,Yamazoe S,Chen JK

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Time point-based integrative analyses of deep-transcriptome identify four signal pathways in blastemal regeneration of zebrafish lower jaw.
Stem cells (Dayton, Ohio)33(3)806-18(2015 Mar)
Zhang H,Wang X,Lyu K,Gao S,Wang G,Fan C,Zhang XA,Yan J