TWIST3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TWIST3
Entrez Gene ID	30176
Full Name	twist3
Synonyms	hm:zeh0190,twist-2,twist2
General protein information	Preferred Names twist3 Names twist homolog 3 Dermo1 twist2l
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130985.3	NP_571060.2	twist homolog 3
NM_130985.2	NP_571060.2	twist homolog 3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	twist3	NP_571060.2
Drosophila melanogaster (fruit fly)	twi	NP_001033967.1
Xenopus tropicalis (tropical clawed frog)	twist2	NP_001096679.1
Gallus gallus (chicken)	TWIST3	NP_001096684.1

Gene Ontology
Bibliography

Related articles in PubMed

Oncogenic BRAF disrupts thyroid morphogenesis and function via twist expression.
Anelli V, Villefranc JA, Chhangawala S, Martinez-McFaline R, Riva E, Nguyen A, Verma A, Bareja R, Chen Z, Scognamiglio T, Elemento O, Houvras Y
eLife6(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The Morphogenesis of Cranial Sutures in Zebrafish.
Topczewska JM, Shoela RA, Tomaszewski JP, Mirmira RB, Gosain AK
PloS one11(11)e0165775(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Sequential and opposing activities of Wnt and BMP coordinate zebrafish bone regeneration.
Stewart S, Gomez AW, Armstrong BE, Henner A, Stankunas K
Cell reports6(3)482-98(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of embryonic twist mRNA expression profiles in zebrafish
Title: Four twist genes in zebrafish, four expression patterns.

The following TWIST3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TWIST3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33639	NM_130985.3 Latest version!	Danio rerio twist3 (twist3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa33639	NM_130985.2	Danio rerio twist3 (twist3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TWIST3 ( NM_130985.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571060.2 Danio rerio twist3 (twist3), mRNA.

Price & Availability↑

CloneID	ODa33639
Clone ID Related Accession (Same CDS sequence)	NM_130985.3 , NM_130985.2
Accession Version	NM_130985.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	twist homolog 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01074080.1 and CABZ01074079.1. On May 31, 2017 this sequence version replaced NM_130985.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078298.1, BK006286.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END##

ATGCGAGAGG AACAGACTTG TGGAGATTTT CCTGAAAGTG GGATCCTTCC CATTGAAGAA 
GAGCAGGAGC GGCGCCCCAA TAAGTGTGCG GTTGTGGTTT CTCCACCGGC GGGCGCACGC 
AAGCGGCTGA CGGGTCCCAA AAAAGAGCCC GTCTCACAAG ACGACAAACC ATCCCTGGAC 
AACCCATCGA ATCTGGCTCC CAAACGTCCC AAAAGAAGCT CTCCGTCATC CTCATCGTCT 
TCTTCGTCCT CTCTGGTGCC CGTCGTGAGT TCGGTTTCTC CAGTTCCCGG ACAGCCCTTC 
GAAGACCTCC ACACGCAACG AGTGATCGCC AACGTTCGGG AACGCCAGCG CACACAGTCC 
CTGAACGACG CCTTCGCCTC CCTCAGAAAG ATCATCCCCA CCTTGCCTTC GGACAAGCTG 
AGCAAGATCC AGATCCTCAA ACTGGCCTCC AGATACATCG ACTTCCTCTA CCAGGTCCTG 
CAGAGCGACG AGATGGACGC CAAGCTGGCC AGCTGCAACT ATCTGGCCCA CGAGAGGCTG 
AGCTACGCCT TCTCCGTCTG GAGGATGGAG GGCGCCTGGT CCATGTCCGC CACTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571060.2
CDS	192..791
Translation	MREEQTCGDFPESGILPIEEEQERRPNKCAVVVSPPAGARKRLTGPKKEPVSQDDKPSLDNPSNLAPKRPKRSSPSSSSSSSSSLVPVVSSVSPVPGQPFEDLHTQRVIANVRERQRTQSLNDAFASLRKIIPTLPSDKLSKIQILKLASRYIDFLYQVLQSDEMDAKLASCNYLAHERLSYAFSVWRMEGAWSMSATH

Target ORF information:

RefSeq Version	NM_130985.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio twist3 (twist3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130985.3

ORF Insert Sequence:

ATGCGAGAGG AACAGACTTG TGGAGATTTT CCTGAAAGTG GGATCCTTCC CATTGAAGAA 
GAGCAGGAGC GGCGCCCCAA TAAGTGTGCG GTTGTGGTTT CTCCACCGGC GGGCGCACGC 
AAGCGGCTGA CGGGTCCCAA AAAAGAGCCC GTCTCACAAG ACGACAAACC ATCCCTGGAC 
AACCCATCGA ATCTGGCTCC CAAACGTCCC AAAAGAAGCT CTCCGTCATC CTCATCGTCT 
TCTTCGTCCT CTCTGGTGCC CGTCGTGAGT TCGGTTTCTC CAGTTCCCGG ACAGCCCTTC 
GAAGACCTCC ACACGCAACG AGTGATCGCC AACGTTCGGG AACGCCAGCG CACACAGTCC 
CTGAACGACG CCTTCGCCTC CCTCAGAAAG ATCATCCCCA CCTTGCCTTC GGACAAGCTG 
AGCAAGATCC AGATCCTCAA ACTGGCCTCC AGATACATCG ACTTCCTCTA CCAGGTCCTG 
CAGAGCGACG AGATGGACGC CAAGCTGGCC AGCTGCAACT ATCTGGCCCA CGAGAGGCTG 
AGCTACGCCT TCTCCGTCTG GAGGATGGAG GGCGCCTGGT CCATGTCCGC CACTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWIST3 ( NM_130985.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571060.2 Danio rerio twist3 (twist3), mRNA.

Price & Availability↑

CloneID	ODa33639
Clone ID Related Accession (Same CDS sequence)	NM_130985.3 , NM_130985.2
Accession Version	NM_130985.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-25
Organism	Danio rerio(Zebrafish)
Product	twist homolog 3
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078298.1. On Aug 1, 2004 this sequence version replaced NM_130985.1. ##Evidence-Data-START## Transcript exon combination :: BC078298.1, BK006286.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCGAGAGG AACAGACTTG TGGAGATTTT CCTGAAAGTG GGATCCTTCC CATTGAAGAA 
GAGCAGGAGC GGCGCCCCAA TAAGTGTGCG GTTGTGGTTT CTCCACCGGC GGGCGCACGC 
AAGCGGCTGA CGGGTCCCAA AAAAGAGCCC GTCTCACAAG ACGACAAACC ATCCCTGGAC 
AACCCATCGA ATCTGGCTCC CAAACGTCCC AAAAGAAGCT CTCCGTCATC CTCATCGTCT 
TCTTCGTCCT CTCTGGTGCC CGTCGTGAGT TCGGTTTCTC CAGTTCCCGG ACAGCCCTTC 
GAAGACCTCC ACACGCAACG AGTGATCGCC AACGTTCGGG AACGCCAGCG CACACAGTCC 
CTGAACGACG CCTTCGCCTC CCTCAGAAAG ATCATCCCCA CCTTGCCTTC GGACAAGCTG 
AGCAAGATCC AGATCCTCAA ACTGGCCTCC AGATACATCG ACTTCCTCTA CCAGGTCCTG 
CAGAGCGACG AGATGGACGC CAAGCTGGCC AGCTGCAACT ATCTGGCCCA CGAGAGGCTG 
AGCTACGCCT TCTCCGTCTG GAGGATGGAG GGCGCCTGGT CCATGTCCGC CACTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571060.2
CDS	200..799
Translation	MREEQTCGDFPESGILPIEEEQERRPNKCAVVVSPPAGARKRLTGPKKEPVSQDDKPSLDNPSNLAPKRPKRSSPSSSSSSSSSLVPVVSSVSPVPGQPFEDLHTQRVIANVRERQRTQSLNDAFASLRKIIPTLPSDKLSKIQILKLASRYIDFLYQVLQSDEMDAKLASCNYLAHERLSYAFSVWRMEGAWSMSATH

Target ORF information:

RefSeq Version	NM_130985.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio twist3 (twist3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130985.2

ORF Insert Sequence:

ATGCGAGAGG AACAGACTTG TGGAGATTTT CCTGAAAGTG GGATCCTTCC CATTGAAGAA 
GAGCAGGAGC GGCGCCCCAA TAAGTGTGCG GTTGTGGTTT CTCCACCGGC GGGCGCACGC 
AAGCGGCTGA CGGGTCCCAA AAAAGAGCCC GTCTCACAAG ACGACAAACC ATCCCTGGAC 
AACCCATCGA ATCTGGCTCC CAAACGTCCC AAAAGAAGCT CTCCGTCATC CTCATCGTCT 
TCTTCGTCCT CTCTGGTGCC CGTCGTGAGT TCGGTTTCTC CAGTTCCCGG ACAGCCCTTC 
GAAGACCTCC ACACGCAACG AGTGATCGCC AACGTTCGGG AACGCCAGCG CACACAGTCC 
CTGAACGACG CCTTCGCCTC CCTCAGAAAG ATCATCCCCA CCTTGCCTTC GGACAAGCTG 
AGCAAGATCC AGATCCTCAA ACTGGCCTCC AGATACATCG ACTTCCTCTA CCAGGTCCTG 
CAGAGCGACG AGATGGACGC CAAGCTGGCC AGCTGCAACT ATCTGGCCCA CGAGAGGCTG 
AGCTACGCCT TCTCCGTCTG GAGGATGGAG GGCGCCTGGT CCATGTCCGC CACTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oncogenic BRAF disrupts thyroid morphogenesis and function via twist expression.
eLife6(2017 03)
Anelli V,Villefranc JA,Chhangawala S,Martinez-McFaline R,Riva E,Nguyen A,Verma A,Bareja R,Chen Z,Scognamiglio T,Elemento O,Houvras Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The Morphogenesis of Cranial Sutures in Zebrafish.
PloS one11(11)e0165775(2016)
Topczewska JM,Shoela RA,Tomaszewski JP,Mirmira RB,Gosain AK

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Sequential and opposing activities of Wnt and BMP coordinate zebrafish bone regeneration.
Cell reports6(3)482-98(2014 Feb)
Stewart S,Gomez AW,Armstrong BE,Henner A,Stankunas K