SI:CH211-132G1.7 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-132G1.7
Entrez Gene ID	101884699
Full Name	si:ch211-132g1.7
General protein information	Preferred Names si:ch211-132g1.7 Names prostaglandin F2 receptor negative regulator
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005168313.4	XP_005168370.1	prostaglandin F2 receptor negative regulator
XM_005168313.3	XP_005168370.1	prostaglandin F2 receptor negative regulator

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PTGFRN	XP_002686168.2
Canis lupus familiaris (dog)	PTGFRN	XP_005630930.1
Mus musculus (house mouse)	Ptgfrn	NP_035327.2
Xenopus tropicalis (tropical clawed frog)	LOC100493252	XP_002943881.2
	PTGFRN	XP_001103382.2
Rattus norvegicus (Norway rat)	Ptgfrn	NP_062116.1
Danio rerio (zebrafish)	si:ch211-132g1.7	XP_005168370.1
Homo sapiens (human)	PTGFRN	NP_065173.2
Pan troglodytes (chimpanzee)	PTGFRN	XP_003308360.1
Gallus gallus (chicken)	PTGFRN	XP_416660.4

Gene Ontology

The following SI:CH211-132G1.7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-132G1.7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05381	XM_005168313.4 Latest version!	Danio rerio si:ch211-132g1.7 (si:ch211-132g1.7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa05381	XM_005168313.3	Danio rerio si:ch211-132g1.7 (si:ch211-132g1.7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-132G1.7 ( XM_005168313.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168370.1 Danio rerio si:ch211-132g1.7 (si:ch211-132g1.7), mRNA.

Price & Availability↑

CloneID	ODa05381
Clone ID Related Accession (Same CDS sequence)	XM_005168313.4 , XM_005168313.3
Accession Version	XM_005168313.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	prostaglandin F2 receptor negative regulator
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168313.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAATGA CACGAGTCCT GATCCTGCTG TACGCCGCTG TCGGTTTATG CAGCTGCCGG 
TCAGTTTCGG TGTCTGGGGG CCCTTTGCTC CGGGTGGAGG GCCAGCCACT GTCCATCCGC 
TGTGATGTGT CCGACTATGA GGGCCCCAGA GAGCAGGACT TTGAATGGAC TGTAACACGA 
GAAACAAAAA CCATCAACGT GATCTCCACA TTCGATCCTG CATTCTCAGA CCGCTCTCTA 
CAGGACCGCA TAAAGACCGG CGACATTAGC ATAGCACGAC TTGCGGACAA TGTAGTGGAA 
CTGAGGATTC AAGAGGCTCA AATTAGCGAC AGCGCCACCT ATCGATGCAG TACTCCAAGC 
ACAGACTCGG TGATAAGCGG AAACTATGAA GCATCTGTTC AGCTGCAAGT TGTTCCCAAC 
ACTCTGATTG TGGCTCCAGA TCCTCCGACG CCTGTAGTTC CCGAAGGCGG ATCCATCAGG 
CTCCAGTGCA GCGTCTCGCG GAGCTTCATC CCGGGCATTT TTCTTTCCAT AACCTGGTCA 
GTGAAGAACC GCTCTTCTCT CCTGGAGGAC CTGCTGACGT TCGGCCCGGA TATGAGCGTG 
ACTGTGGACA CCGTATCCTC TCAGCGCTAC ACAGATGGTG GGATGCGATT AGAGATGGGA 
AACGGAGTCT CGCACAGTCT GGTTCTCTCT GGAGTGACGC CGGCTGATCA GGGCCTGTAT 
ATGTGCACCG CCAGCCTGTG GACCAGAGAG CAGGGGATCT GGAGCCGCAT TCAGGAGAAG 
AGCATGGAGA TGGGGGACGT TACGGTCACA CCTGCTGTGA ACTCACTCGC GGTTCATGTT 
CAGAACGACT CCACATTATC CACTGGAGAT TCGCTGGTGC TCTTGTGTTC GGTGATGGTG 
TCCGGTGCGT CTGCGGTGGA TCTGGACGTG ACGTGGCTGC AGAACGACAC GCATGTTCTG 
GGACATCTGA GTCGAGACGG CGTTCAGACT GACAGCAGCA GCAGAGCAGT GGATGTAAAG 
CGTTTAGGAA AATGGGATTA TCAGTTGAAG ATTCAGGATG TGGAGACGTC TGATGGAGGA 
TTGTATACAT GCAAAGTGAA TGTGTGGATT CAGCGTGACA CTGGGCGATG GTATAAGGCT 
GCAGAGAAGA TTTCTGACCC TGTTCGAGTC CATGTTATTA AGCACAATCC GGAGTACACT 
GCTGTTCTGA GCTCCTCTGT GACTGCAAAG TTTTCCGGCG ACCCAACTGA GTTGGAATGT 
AAAGTTATGA ACGTGTCGAA TCTGCGCAGC GGCAGACTGG GAGTGTCGTG GCTCTACAGA 
GATCTTCAGC CTAATGGTAC TCCAGGTTCG ACTCAAACCG TCGCTTCTTT GGATGAAAAC 
GGGACTCTTG TTCCAGGTAG GGCGTTTAAG AGCCGTGTGG AGGCGGGGCT TATCACAGCC 
ACCCGGGCGG AGCCTTCCAT GTTTAAGCTC CGCCTCCTGC ACACAACGGA TGCAGATGCT 
GGTGAATATA TGTGTGTAGT GAGCGCGTGG ATCCCATCTC CACATGGACA CTGGAAAAAG 
GTGGCAGAGC ATCTGACACC GGCAGTTAAA GTCAGCTTCG CTAACAAGCG TCCTGTTCTG 
GGTGTGGTGG CTCGTCGTCT CCGTGAAGCC ACAGCCGTGG GCTCCACATT TGAGATGAGC 
TGCCAGGCCA GTGTCCAGAA CCTTCCGTCT GGATCCGCGT TCTCCATCTC TGTGCTGTCT 
GAGGAGTCCG TAGGAGCATC CGGCCGCAGA CGGGCCTCGC TGGACCCTGA CCTGCTCTTG 
AAGCTGGAGG ATGTTGGCGA GCCGGCCCGG AGCCACAGCC TGATGCTGCT GAAGACAAAG 
AAGACGGAGT TTGAGTTTCG CATCCCGGCA GTTCGGCTGA GTGACCGCGG CTTTTACTAC 
TGCGAGATGA AGGCCTGGAC CAGGCAGATC GGGGACGAAT GGGTGGAAGT GGCCAAAGGA 
CTTTCCAATA AAGTCCAGAT CGCCTTCACA CACAAAGGTC CCACGTTTGA TGTGTCCATC 
AGCTCGGACG TGAGCAGTGT TTTCCCCTGG GAAACGGTGA AGATGGAGTG TGACGTCAGT 
GTTAGCGGCA CGTCTCCATC CGCAGATGAC GTGGCGTACG AGCTGAAGTG GTTTCTGAGT 
CGTCTGCGTG GCTCCAGCAG CCCGACTCTG TTAGCCAGCA TGGACCGCTG GGGCGTCGTG 
CGTAAAAGTC CTCGCAACGA TAGCAGCGAC TGCAGCCTGG AGCGTCTGGA GGCCCGAAGA 
TTCGCTCTGA GCGTCCACAA CACGCAGGAT TCAGACGCTG GAGACTATCA CTGCAGCGTC 
ACGCCGTGGG TCCGCTCGTC CGTCAATGGC GTCTGGAGCA GAGCAGCTGA AATCTCCTCA 
CAGAGAGTCT TCCTCAACGT CAGGTTCGCA TTGTGGTCCT CCATGAAGCT GCCACTGCTG 
TACGGCATCT GTGCTTCAGC AACTGTAGGT GTTGTTTCTC TCATCCTGGG CCTGGTCTGC 
GCTCACTGCT GCTGTAAAAC CTCCACACCC GCTACTCCTC GCTCTCGCAA ACTCATGGAG 
CTGGAGATGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168370.1
CDS	255..2849
Translation	MGMTRVLILLYAAVGLCSCRSVSVSGGPLLRVEGQPLSIRCDVSDYEGPREQDFEWTVTRETKTINVISTFDPAFSDRSLQDRIKTGDISIARLADNVVELRIQEAQISDSATYRCSTPSTDSVISGNYEASVQLQVVPNTLIVAPDPPTPVVPEGGSIRLQCSVSRSFIPGIFLSITWSVKNRSSLLEDLLTFGPDMSVTVDTVSSQRYTDGGMRLEMGNGVSHSLVLSGVTPADQGLYMCTASLWTREQGIWSRIQEKSMEMGDVTVTPAVNSLAVHVQNDSTLSTGDSLVLLCSVMVSGASAVDLDVTWLQNDTHVLGHLSRDGVQTDSSSRAVDVKRLGKWDYQLKIQDVETSDGGLYTCKVNVWIQRDTGRWYKAAEKISDPVRVHVIKHNPEYTAVLSSSVTAKFSGDPTELECKVMNVSNLRSGRLGVSWLYRDLQPNGTPGSTQTVASLDENGTLVPGRAFKSRVEAGLITATRAEPSMFKLRLLHTTDADAGEYMCVVSAWIPSPHGHWKKVAEHLTPAVKVSFANKRPVLGVVARRLREATAVGSTFEMSCQASVQNLPSGSAFSISVLSEESVGASGRRRASLDPDLLLKLEDVGEPARSHSLMLLKTKKTEFEFRIPAVRLSDRGFYYCEMKAWTRQIGDEWVEVAKGLSNKVQIAFTHKGPTFDVSISSDVSSVFPWETVKMECDVSVSGTSPSADDVAYELKWFLSRLRGSSSPTLLASMDRWGVVRKSPRNDSSDCSLERLEARRFALSVHNTQDSDAGDYHCSVTPWVRSSVNGVWSRAAEISSQRVFLNVRFALWSSMKLPLLYGICASATVGVVSLILGLVCAHCCCKTSTPATPRSRKLMELEMD

Target ORF information:

RefSeq Version	XM_005168313.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-132g1.7 (si:ch211-132g1.7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168313.4

ORF Insert Sequence:

ATGGGAATGA CACGAGTCCT GATCCTGCTG TACGCCGCTG TCGGTTTATG CAGCTGCCGG 
TCAGTTTCGG TGTCTGGGGG CCCTTTGCTC CGGGTGGAGG GCCAGCCACT GTCCATCCGC 
TGTGATGTGT CCGACTATGA GGGCCCCAGA GAGCAGGACT TTGAATGGAC TGTAACACGA 
GAAACAAAAA CCATCAACGT GATCTCCACA TTCGATCCTG CATTCTCAGA CCGCTCTCTA 
CAGGACCGCA TAAAGACCGG CGACATTAGC ATAGCACGAC TTGCGGACAA TGTAGTGGAA 
CTGAGGATTC AAGAGGCTCA AATTAGCGAC AGCGCCACCT ATCGATGCAG TACTCCAAGC 
ACAGACTCGG TGATAAGCGG AAACTATGAA GCATCTGTTC AGCTGCAAGT TGTTCCCAAC 
ACTCTGATTG TGGCTCCAGA TCCTCCGACG CCTGTAGTTC CCGAAGGCGG ATCCATCAGG 
CTCCAGTGCA GCGTCTCGCG GAGCTTCATC CCGGGCATTT TTCTTTCCAT AACCTGGTCA 
GTGAAGAACC GCTCTTCTCT CCTGGAGGAC CTGCTGACGT TCGGCCCGGA TATGAGCGTG 
ACTGTGGACA CCGTATCCTC TCAGCGCTAC ACAGATGGTG GGATGCGATT AGAGATGGGA 
AACGGAGTCT CGCACAGTCT GGTTCTCTCT GGAGTGACGC CGGCTGATCA GGGCCTGTAT 
ATGTGCACCG CCAGCCTGTG GACCAGAGAG CAGGGGATCT GGAGCCGCAT TCAGGAGAAG 
AGCATGGAGA TGGGGGACGT TACGGTCACA CCTGCTGTGA ACTCACTCGC GGTTCATGTT 
CAGAACGACT CCACATTATC CACTGGAGAT TCGCTGGTGC TCTTGTGTTC GGTGATGGTG 
TCCGGTGCGT CTGCGGTGGA TCTGGACGTG ACGTGGCTGC AGAACGACAC GCATGTTCTG 
GGACATCTGA GTCGAGACGG CGTTCAGACT GACAGCAGCA GCAGAGCAGT GGATGTAAAG 
CGTTTAGGAA AATGGGATTA TCAGTTGAAG ATTCAGGATG TGGAGACGTC TGATGGAGGA 
TTGTATACAT GCAAAGTGAA TGTGTGGATT CAGCGTGACA CTGGGCGATG GTATAAGGCT 
GCAGAGAAGA TTTCTGACCC TGTTCGAGTC CATGTTATTA AGCACAATCC GGAGTACACT 
GCTGTTCTGA GCTCCTCTGT GACTGCAAAG TTTTCCGGCG ACCCAACTGA GTTGGAATGT 
AAAGTTATGA ACGTGTCGAA TCTGCGCAGC GGCAGACTGG GAGTGTCGTG GCTCTACAGA 
GATCTTCAGC CTAATGGTAC TCCAGGTTCG ACTCAAACCG TCGCTTCTTT GGATGAAAAC 
GGGACTCTTG TTCCAGGTAG GGCGTTTAAG AGCCGTGTGG AGGCGGGGCT TATCACAGCC 
ACCCGGGCGG AGCCTTCCAT GTTTAAGCTC CGCCTCCTGC ACACAACGGA TGCAGATGCT 
GGTGAATATA TGTGTGTAGT GAGCGCGTGG ATCCCATCTC CACATGGACA CTGGAAAAAG 
GTGGCAGAGC ATCTGACACC GGCAGTTAAA GTCAGCTTCG CTAACAAGCG TCCTGTTCTG 
GGTGTGGTGG CTCGTCGTCT CCGTGAAGCC ACAGCCGTGG GCTCCACATT TGAGATGAGC 
TGCCAGGCCA GTGTCCAGAA CCTTCCGTCT GGATCCGCGT TCTCCATCTC TGTGCTGTCT 
GAGGAGTCCG TAGGAGCATC CGGCCGCAGA CGGGCCTCGC TGGACCCTGA CCTGCTCTTG 
AAGCTGGAGG ATGTTGGCGA GCCGGCCCGG AGCCACAGCC TGATGCTGCT GAAGACAAAG 
AAGACGGAGT TTGAGTTTCG CATCCCGGCA GTTCGGCTGA GTGACCGCGG CTTTTACTAC 
TGCGAGATGA AGGCCTGGAC CAGGCAGATC GGGGACGAAT GGGTGGAAGT GGCCAAAGGA 
CTTTCCAATA AAGTCCAGAT CGCCTTCACA CACAAAGGTC CCACGTTTGA TGTGTCCATC 
AGCTCGGACG TGAGCAGTGT TTTCCCCTGG GAAACGGTGA AGATGGAGTG TGACGTCAGT 
GTTAGCGGCA CGTCTCCATC CGCAGATGAC GTGGCGTACG AGCTGAAGTG GTTTCTGAGT 
CGTCTGCGTG GCTCCAGCAG CCCGACTCTG TTAGCCAGCA TGGACCGCTG GGGCGTCGTG 
CGTAAAAGTC CTCGCAACGA TAGCAGCGAC TGCAGCCTGG AGCGTCTGGA GGCCCGAAGA 
TTCGCTCTGA GCGTCCACAA CACGCAGGAT TCAGACGCTG GAGACTATCA CTGCAGCGTC 
ACGCCGTGGG TCCGCTCGTC CGTCAATGGC GTCTGGAGCA GAGCAGCTGA AATCTCCTCA 
CAGAGAGTCT TCCTCAACGT CAGGTTCGCA TTGTGGTCCT CCATGAAGCT GCCACTGCTG 
TACGGCATCT GTGCTTCAGC AACTGTAGGT GTTGTTTCTC TCATCCTGGG CCTGGTCTGC 
GCTCACTGCT GCTGTAAAAC CTCCACACCC GCTACTCCTC GCTCTCGCAA ACTCATGGAG 
CTGGAGATGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-132G1.7 ( XM_005168313.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168370.1 Danio rerio si:ch211-132g1.7 (si:ch211-132g1.7), mRNA.

Price & Availability↑

CloneID	ODa05381
Clone ID Related Accession (Same CDS sequence)	XM_005168313.4 , XM_005168313.3
Accession Version	XM_005168313.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	prostaglandin F2 receptor negative regulator
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168313.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAATGA CACGAGTCCT GATCCTGCTG TACGCCGCTG TCGGTTTATG CAGCTGCCGG 
TCAGTTTCGG TGTCTGGGGG CCCTTTGCTC CGGGTGGAGG GCCAGCCACT GTCCATCCGC 
TGTGATGTGT CCGACTATGA GGGCCCCAGA GAGCAGGACT TTGAATGGAC TGTAACACGA 
GAAACAAAAA CCATCAACGT GATCTCCACA TTCGATCCTG CATTCTCAGA CCGCTCTCTA 
CAGGACCGCA TAAAGACCGG CGACATTAGC ATAGCACGAC TTGCGGACAA TGTAGTGGAA 
CTGAGGATTC AAGAGGCTCA AATTAGCGAC AGCGCCACCT ATCGATGCAG TACTCCAAGC 
ACAGACTCGG TGATAAGCGG AAACTATGAA GCATCTGTTC AGCTGCAAGT TGTTCCCAAC 
ACTCTGATTG TGGCTCCAGA TCCTCCGACG CCTGTAGTTC CCGAAGGCGG ATCCATCAGG 
CTCCAGTGCA GCGTCTCGCG GAGCTTCATC CCGGGCATTT TTCTTTCCAT AACCTGGTCA 
GTGAAGAACC GCTCTTCTCT CCTGGAGGAC CTGCTGACGT TCGGCCCGGA TATGAGCGTG 
ACTGTGGACA CCGTATCCTC TCAGCGCTAC ACAGATGGTG GGATGCGATT AGAGATGGGA 
AACGGAGTCT CGCACAGTCT GGTTCTCTCT GGAGTGACGC CGGCTGATCA GGGCCTGTAT 
ATGTGCACCG CCAGCCTGTG GACCAGAGAG CAGGGGATCT GGAGCCGCAT TCAGGAGAAG 
AGCATGGAGA TGGGGGACGT TACGGTCACA CCTGCTGTGA ACTCACTCGC GGTTCATGTT 
CAGAACGACT CCACATTATC CACTGGAGAT TCGCTGGTGC TCTTGTGTTC GGTGATGGTG 
TCCGGTGCGT CTGCGGTGGA TCTGGACGTG ACGTGGCTGC AGAACGACAC GCATGTTCTG 
GGACATCTGA GTCGAGACGG CGTTCAGACT GACAGCAGCA GCAGAGCAGT GGATGTAAAG 
CGTTTAGGAA AATGGGATTA TCAGTTGAAG ATTCAGGATG TGGAGACGTC TGATGGAGGA 
TTGTATACAT GCAAAGTGAA TGTGTGGATT CAGCGTGACA CTGGGCGATG GTATAAGGCT 
GCAGAGAAGA TTTCTGACCC TGTTCGAGTC CATGTTATTA AGCACAATCC GGAGTACACT 
GCTGTTCTGA GCTCCTCTGT GACTGCAAAG TTTTCCGGCG ACCCAACTGA GTTGGAATGT 
AAAGTTATGA ACGTGTCGAA TCTGCGCAGC GGCAGACTGG GAGTGTCGTG GCTCTACAGA 
GATCTTCAGC CTAATGGTAC TCCAGGTTCG ACTCAAACCG TCGCTTCTTT GGATGAAAAC 
GGGACTCTTG TTCCAGGTAG GGCGTTTAAG AGCCGTGTGG AGGCGGGGCT TATCACAGCC 
ACCCGGGCGG AGCCTTCCAT GTTTAAGCTC CGCCTCCTGC ACACAACGGA TGCAGATGCT 
GGTGAATATA TGTGTGTAGT GAGCGCGTGG ATCCCATCTC CACATGGACA CTGGAAAAAG 
GTGGCAGAGC ATCTGACACC GGCAGTTAAA GTCAGCTTCG CTAACAAGCG TCCTGTTCTG 
GGTGTGGTGG CTCGTCGTCT CCGTGAAGCC ACAGCCGTGG GCTCCACATT TGAGATGAGC 
TGCCAGGCCA GTGTCCAGAA CCTTCCGTCT GGATCCGCGT TCTCCATCTC TGTGCTGTCT 
GAGGAGTCCG TAGGAGCATC CGGCCGCAGA CGGGCCTCGC TGGACCCTGA CCTGCTCTTG 
AAGCTGGAGG ATGTTGGCGA GCCGGCCCGG AGCCACAGCC TGATGCTGCT GAAGACAAAG 
AAGACGGAGT TTGAGTTTCG CATCCCGGCA GTTCGGCTGA GTGACCGCGG CTTTTACTAC 
TGCGAGATGA AGGCCTGGAC CAGGCAGATC GGGGACGAAT GGGTGGAAGT GGCCAAAGGA 
CTTTCCAATA AAGTCCAGAT CGCCTTCACA CACAAAGGTC CCACGTTTGA TGTGTCCATC 
AGCTCGGACG TGAGCAGTGT TTTCCCCTGG GAAACGGTGA AGATGGAGTG TGACGTCAGT 
GTTAGCGGCA CGTCTCCATC CGCAGATGAC GTGGCGTACG AGCTGAAGTG GTTTCTGAGT 
CGTCTGCGTG GCTCCAGCAG CCCGACTCTG TTAGCCAGCA TGGACCGCTG GGGCGTCGTG 
CGTAAAAGTC CTCGCAACGA TAGCAGCGAC TGCAGCCTGG AGCGTCTGGA GGCCCGAAGA 
TTCGCTCTGA GCGTCCACAA CACGCAGGAT TCAGACGCTG GAGACTATCA CTGCAGCGTC 
ACGCCGTGGG TCCGCTCGTC CGTCAATGGC GTCTGGAGCA GAGCAGCTGA AATCTCCTCA 
CAGAGAGTCT TCCTCAACGT CAGGTTCGCA TTGTGGTCCT CCATGAAGCT GCCACTGCTG 
TACGGCATCT GTGCTTCAGC AACTGTAGGT GTTGTTTCTC TCATCCTGGG CCTGGTCTGC 
GCTCACTGCT GCTGTAAAAC CTCCACACCC GCTACTCCTC GCTCTCGCAA ACTCATGGAG 
CTGGAGATGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168370.1
CDS	227..2821
Translation	MGMTRVLILLYAAVGLCSCRSVSVSGGPLLRVEGQPLSIRCDVSDYEGPREQDFEWTVTRETKTINVISTFDPAFSDRSLQDRIKTGDISIARLADNVVELRIQEAQISDSATYRCSTPSTDSVISGNYEASVQLQVVPNTLIVAPDPPTPVVPEGGSIRLQCSVSRSFIPGIFLSITWSVKNRSSLLEDLLTFGPDMSVTVDTVSSQRYTDGGMRLEMGNGVSHSLVLSGVTPADQGLYMCTASLWTREQGIWSRIQEKSMEMGDVTVTPAVNSLAVHVQNDSTLSTGDSLVLLCSVMVSGASAVDLDVTWLQNDTHVLGHLSRDGVQTDSSSRAVDVKRLGKWDYQLKIQDVETSDGGLYTCKVNVWIQRDTGRWYKAAEKISDPVRVHVIKHNPEYTAVLSSSVTAKFSGDPTELECKVMNVSNLRSGRLGVSWLYRDLQPNGTPGSTQTVASLDENGTLVPGRAFKSRVEAGLITATRAEPSMFKLRLLHTTDADAGEYMCVVSAWIPSPHGHWKKVAEHLTPAVKVSFANKRPVLGVVARRLREATAVGSTFEMSCQASVQNLPSGSAFSISVLSEESVGASGRRRASLDPDLLLKLEDVGEPARSHSLMLLKTKKTEFEFRIPAVRLSDRGFYYCEMKAWTRQIGDEWVEVAKGLSNKVQIAFTHKGPTFDVSISSDVSSVFPWETVKMECDVSVSGTSPSADDVAYELKWFLSRLRGSSSPTLLASMDRWGVVRKSPRNDSSDCSLERLEARRFALSVHNTQDSDAGDYHCSVTPWVRSSVNGVWSRAAEISSQRVFLNVRFALWSSMKLPLLYGICASATVGVVSLILGLVCAHCCCKTSTPATPRSRKLMELEMD

Target ORF information:

RefSeq Version	XM_005168313.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-132g1.7 (si:ch211-132g1.7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168313.3

ORF Insert Sequence:

ATGGGAATGA CACGAGTCCT GATCCTGCTG TACGCCGCTG TCGGTTTATG CAGCTGCCGG 
TCAGTTTCGG TGTCTGGGGG CCCTTTGCTC CGGGTGGAGG GCCAGCCACT GTCCATCCGC 
TGTGATGTGT CCGACTATGA GGGCCCCAGA GAGCAGGACT TTGAATGGAC TGTAACACGA 
GAAACAAAAA CCATCAACGT GATCTCCACA TTCGATCCTG CATTCTCAGA CCGCTCTCTA 
CAGGACCGCA TAAAGACCGG CGACATTAGC ATAGCACGAC TTGCGGACAA TGTAGTGGAA 
CTGAGGATTC AAGAGGCTCA AATTAGCGAC AGCGCCACCT ATCGATGCAG TACTCCAAGC 
ACAGACTCGG TGATAAGCGG AAACTATGAA GCATCTGTTC AGCTGCAAGT TGTTCCCAAC 
ACTCTGATTG TGGCTCCAGA TCCTCCGACG CCTGTAGTTC CCGAAGGCGG ATCCATCAGG 
CTCCAGTGCA GCGTCTCGCG GAGCTTCATC CCGGGCATTT TTCTTTCCAT AACCTGGTCA 
GTGAAGAACC GCTCTTCTCT CCTGGAGGAC CTGCTGACGT TCGGCCCGGA TATGAGCGTG 
ACTGTGGACA CCGTATCCTC TCAGCGCTAC ACAGATGGTG GGATGCGATT AGAGATGGGA 
AACGGAGTCT CGCACAGTCT GGTTCTCTCT GGAGTGACGC CGGCTGATCA GGGCCTGTAT 
ATGTGCACCG CCAGCCTGTG GACCAGAGAG CAGGGGATCT GGAGCCGCAT TCAGGAGAAG 
AGCATGGAGA TGGGGGACGT TACGGTCACA CCTGCTGTGA ACTCACTCGC GGTTCATGTT 
CAGAACGACT CCACATTATC CACTGGAGAT TCGCTGGTGC TCTTGTGTTC GGTGATGGTG 
TCCGGTGCGT CTGCGGTGGA TCTGGACGTG ACGTGGCTGC AGAACGACAC GCATGTTCTG 
GGACATCTGA GTCGAGACGG CGTTCAGACT GACAGCAGCA GCAGAGCAGT GGATGTAAAG 
CGTTTAGGAA AATGGGATTA TCAGTTGAAG ATTCAGGATG TGGAGACGTC TGATGGAGGA 
TTGTATACAT GCAAAGTGAA TGTGTGGATT CAGCGTGACA CTGGGCGATG GTATAAGGCT 
GCAGAGAAGA TTTCTGACCC TGTTCGAGTC CATGTTATTA AGCACAATCC GGAGTACACT 
GCTGTTCTGA GCTCCTCTGT GACTGCAAAG TTTTCCGGCG ACCCAACTGA GTTGGAATGT 
AAAGTTATGA ACGTGTCGAA TCTGCGCAGC GGCAGACTGG GAGTGTCGTG GCTCTACAGA 
GATCTTCAGC CTAATGGTAC TCCAGGTTCG ACTCAAACCG TCGCTTCTTT GGATGAAAAC 
GGGACTCTTG TTCCAGGTAG GGCGTTTAAG AGCCGTGTGG AGGCGGGGCT TATCACAGCC 
ACCCGGGCGG AGCCTTCCAT GTTTAAGCTC CGCCTCCTGC ACACAACGGA TGCAGATGCT 
GGTGAATATA TGTGTGTAGT GAGCGCGTGG ATCCCATCTC CACATGGACA CTGGAAAAAG 
GTGGCAGAGC ATCTGACACC GGCAGTTAAA GTCAGCTTCG CTAACAAGCG TCCTGTTCTG 
GGTGTGGTGG CTCGTCGTCT CCGTGAAGCC ACAGCCGTGG GCTCCACATT TGAGATGAGC 
TGCCAGGCCA GTGTCCAGAA CCTTCCGTCT GGATCCGCGT TCTCCATCTC TGTGCTGTCT 
GAGGAGTCCG TAGGAGCATC CGGCCGCAGA CGGGCCTCGC TGGACCCTGA CCTGCTCTTG 
AAGCTGGAGG ATGTTGGCGA GCCGGCCCGG AGCCACAGCC TGATGCTGCT GAAGACAAAG 
AAGACGGAGT TTGAGTTTCG CATCCCGGCA GTTCGGCTGA GTGACCGCGG CTTTTACTAC 
TGCGAGATGA AGGCCTGGAC CAGGCAGATC GGGGACGAAT GGGTGGAAGT GGCCAAAGGA 
CTTTCCAATA AAGTCCAGAT CGCCTTCACA CACAAAGGTC CCACGTTTGA TGTGTCCATC 
AGCTCGGACG TGAGCAGTGT TTTCCCCTGG GAAACGGTGA AGATGGAGTG TGACGTCAGT 
GTTAGCGGCA CGTCTCCATC CGCAGATGAC GTGGCGTACG AGCTGAAGTG GTTTCTGAGT 
CGTCTGCGTG GCTCCAGCAG CCCGACTCTG TTAGCCAGCA TGGACCGCTG GGGCGTCGTG 
CGTAAAAGTC CTCGCAACGA TAGCAGCGAC TGCAGCCTGG AGCGTCTGGA GGCCCGAAGA 
TTCGCTCTGA GCGTCCACAA CACGCAGGAT TCAGACGCTG GAGACTATCA CTGCAGCGTC 
ACGCCGTGGG TCCGCTCGTC CGTCAATGGC GTCTGGAGCA GAGCAGCTGA AATCTCCTCA 
CAGAGAGTCT TCCTCAACGT CAGGTTCGCA TTGTGGTCCT CCATGAAGCT GCCACTGCTG 
TACGGCATCT GTGCTTCAGC AACTGTAGGT GTTGTTTCTC TCATCCTGGG CCTGGTCTGC 
GCTCACTGCT GCTGTAAAAC CTCCACACCC GCTACTCCTC GCTCTCGCAA ACTCATGGAG 
CTGGAGATGG ATTGA