ism2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ism2a
Entrez Gene ID	100535376
Full Name	isthmin 2a
Synonyms	si:dkey-106a20.2
General protein information	Preferred Names isthmin 2a Names isthmin-2-like zmp:0000000969
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003200340.4	XP_003200388.1	isthmin-2-like
XM_003200340.5	XP_003200388.1	isthmin-2-like

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ism2	XP_006240466.1
Pan troglodytes (chimpanzee)	ISM2	XP_003314481.1
Danio rerio (zebrafish)	LOC100535376	XP_003200388.1
Bos taurus (cattle)	ISM2	XP_003586648.2
Mus musculus (house mouse)	Ism2	XP_006516495.1
Gallus gallus (chicken)	ISM2	XP_426452.2
	LOC706425	XP_002805183.1
Canis lupus familiaris (dog)	ISM2	XP_005623765.1
Xenopus tropicalis (tropical clawed frog)	ism2	NP_001116489.1

Gene Ontology

The following ism2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ism2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22404	XM_003200340.5 Latest version!	Danio rerio isthmin 2a (ism2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa22404	XM_003200340.4	Danio rerio isthmin 2a (ism2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ism2a ( XM_003200340.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200388.1 Danio rerio isthmin 2a (ism2a), mRNA.

Price & Availability↑

CloneID	ODa22404
Clone ID Related Accession (Same CDS sequence)	XM_003200340.4 , XM_003200340.5
Accession Version	XM_003200340.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	isthmin-2-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200340.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATTC GAAGCCAGGC TTTACGGTGG GCAGTTTTCT TGACACTTTG TGCTCTGATG 
GTGAAGGGCT TCCCAGCAAA AAGACGCAGA GCAAATCACA TCAAGAGCAA GAGTGGTGCA 
TCTCTGGAGG TTAGAGGTCA TGGTGGCTCC AAGGATGTCA AGCAGTCTTT GCAAGACATT 
CAGGGTCAAA ACCAACTAAG GAGCGAGTTG CCTTTGCCTC GCCGTCATAA AAGGAGATGG 
TCACGGATTC ACTCGGAGGG AGTCCTTCCC AAACCTGCTC CAGAGGAAGA AGAGGAAACC 
TTCATTTTAG ACCTGAAGAA CTTCCCTGAC CTTGCCAATG CAGACCTGGG CTCCCAGAAC 
CCAAACATTC AAGTGACCAT TGAGGTTGTG GACAGCCCAC AGATGGAGAT TGAGATGGAT 
CTGGCCAAGG AGAGCAGGAA CGACTGGTCC TTGAGCTCGG AGGAATGGCT AGGCCATAAA 
AAGCTCTTCT GGCCACTGTT TTGGGAATAC CACGACTCCA GTCAGGAAGG TCCCGGGCAA 
GGCAGTCTGG AGGAGAATGA GGAGGACCTC ATCTATGAAG GAGAAGACTC CTTTTTGAGT 
GGTGTGGGTG CAGACTGGAA CAGTCGATGG AAGGGCTGGG ACTCTACGGA CAATTACGAA 
TATGAGGAGG AAGAATGGAG TGATTGGTCA CCATGTAGTG CCACCTGTGG ACAGGGGAAT 
CAGAAGAGGA TTCGCTCGTG TGGCTATGCC TGCACTGCTA CAGAGTCACG GACATGTGAT 
TTAGAGAGAT GTCCTGATGA CATAATTCCT GTGACGGAAC CAAGTCCTCA TGAAATGGCA 
AACAACACGG AGCTTTTGGG AACAGATGTT GACAGCTGTG AGAAGTGGCT GAACTGCAAG 
AACGACTTCC TCCAGAAATA CCTACACAAA GTCCTGACTG AGTTACCCAA TTGCCCATGT 
GTCTACCCAT CCGAGGTGGT CTACAGTGCT GTCAACGTTT TTGACAGGAA GCTGCAGAAG 
ACCTATCGAT GGCGAGATGC CAGCGGCCCC AAAGAGAGAC TGGACATCTA CAAGCCCTCC 
GCCAGGTTCT GCATTCGCTC CATGCTCTCC TTCGACAGCA CTACTCTGGC GGCACAACAT 
TGCTGCTACG ATGATCACAT GAAGCTCATC ACACGAGGAA AAGGTGCCGG GGCCCCAAAC 
CTCATCAGTA CAGAGTTCTC CCCGGAGCTT CATTACAAAG TGGACGTGCT CCCATGGATC 
CTGTGTAAAG GAGACTGGAG CCGCTTTCAT TCGGTTCGGC CTCCCAATAA CGGTTTACAG 
TGTGTTGACA ACCCACAGGA AAACGTCTAT ATGAATGAGC TAGAAGAGGC CAGAGAGTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200388.1
CDS	500..1882
Translation	MLIRSQALRWAVFLTLCALMVKGFPAKRRRANHIKSKSGASLEVRGHGGSKDVKQSLQDIQGQNQLRSELPLPRRHKRRWSRIHSEGVLPKPAPEEEEETFILDLKNFPDLANADLGSQNPNIQVTIEVVDSPQMEIEMDLAKESRNDWSLSSEEWLGHKKLFWPLFWEYHDSSQEGPGQGSLEENEEDLIYEGEDSFLSGVGADWNSRWKGWDSTDNYEYEEEEWSDWSPCSATCGQGNQKRIRSCGYACTATESRTCDLERCPDDIIPVTEPSPHEMANNTELLGTDVDSCEKWLNCKNDFLQKYLHKVLTELPNCPCVYPSEVVYSAVNVFDRKLQKTYRWRDASGPKERLDIYKPSARFCIRSMLSFDSTTLAAQHCCYDDHMKLITRGKGAGAPNLISTEFSPELHYKVDVLPWILCKGDWSRFHSVRPPNNGLQCVDNPQENVYMNELEEAREY

Target ORF information:

RefSeq Version	XM_003200340.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio isthmin 2a (ism2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200340.4

ORF Insert Sequence:

ATGCTGATTC GAAGCCAGGC TTTACGGTGG GCAGTTTTCT TGACACTTTG TGCTCTGATG 
GTGAAGGGCT TCCCAGCAAA AAGACGCAGA GCAAATCACA TCAAGAGCAA GAGTGGTGCA 
TCTCTGGAGG TTAGAGGTCA TGGTGGCTCC AAGGATGTCA AGCAGTCTTT GCAAGACATT 
CAGGGTCAAA ACCAACTAAG GAGCGAGTTG CCTTTGCCTC GCCGTCATAA AAGGAGATGG 
TCACGGATTC ACTCGGAGGG AGTCCTTCCC AAACCTGCTC CAGAGGAAGA AGAGGAAACC 
TTCATTTTAG ACCTGAAGAA CTTCCCTGAC CTTGCCAATG CAGACCTGGG CTCCCAGAAC 
CCAAACATTC AAGTGACCAT TGAGGTTGTG GACAGCCCAC AGATGGAGAT TGAGATGGAT 
CTGGCCAAGG AGAGCAGGAA CGACTGGTCC TTGAGCTCGG AGGAATGGCT AGGCCATAAA 
AAGCTCTTCT GGCCACTGTT TTGGGAATAC CACGACTCCA GTCAGGAAGG TCCCGGGCAA 
GGCAGTCTGG AGGAGAATGA GGAGGACCTC ATCTATGAAG GAGAAGACTC CTTTTTGAGT 
GGTGTGGGTG CAGACTGGAA CAGTCGATGG AAGGGCTGGG ACTCTACGGA CAATTACGAA 
TATGAGGAGG AAGAATGGAG TGATTGGTCA CCATGTAGTG CCACCTGTGG ACAGGGGAAT 
CAGAAGAGGA TTCGCTCGTG TGGCTATGCC TGCACTGCTA CAGAGTCACG GACATGTGAT 
TTAGAGAGAT GTCCTGATGA CATAATTCCT GTGACGGAAC CAAGTCCTCA TGAAATGGCA 
AACAACACGG AGCTTTTGGG AACAGATGTT GACAGCTGTG AGAAGTGGCT GAACTGCAAG 
AACGACTTCC TCCAGAAATA CCTACACAAA GTCCTGACTG AGTTACCCAA TTGCCCATGT 
GTCTACCCAT CCGAGGTGGT CTACAGTGCT GTCAACGTTT TTGACAGGAA GCTGCAGAAG 
ACCTATCGAT GGCGAGATGC CAGCGGCCCC AAAGAGAGAC TGGACATCTA CAAGCCCTCC 
GCCAGGTTCT GCATTCGCTC CATGCTCTCC TTCGACAGCA CTACTCTGGC GGCACAACAT 
TGCTGCTACG ATGATCACAT GAAGCTCATC ACACGAGGAA AAGGTGCCGG GGCCCCAAAC 
CTCATCAGTA CAGAGTTCTC CCCGGAGCTT CATTACAAAG TGGACGTGCT CCCATGGATC 
CTGTGTAAAG GAGACTGGAG CCGCTTTCAT TCGGTTCGGC CTCCCAATAA CGGTTTACAG 
TGTGTTGACA ACCCACAGGA AAACGTCTAT ATGAATGAGC TAGAAGAGGC CAGAGAGTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ism2a ( XM_003200340.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200388.1 Danio rerio isthmin 2a (ism2a), mRNA.

Price & Availability↑

CloneID	ODa22404
Clone ID Related Accession (Same CDS sequence)	XM_003200340.4 , XM_003200340.5
Accession Version	XM_003200340.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	isthmin-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200340.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATTC GAAGCCAGGC TTTACGGTGG GCAGTTTTCT TGACACTTTG TGCTCTGATG 
GTGAAGGGCT TCCCAGCAAA AAGACGCAGA GCAAATCACA TCAAGAGCAA GAGTGGTGCA 
TCTCTGGAGG TTAGAGGTCA TGGTGGCTCC AAGGATGTCA AGCAGTCTTT GCAAGACATT 
CAGGGTCAAA ACCAACTAAG GAGCGAGTTG CCTTTGCCTC GCCGTCATAA AAGGAGATGG 
TCACGGATTC ACTCGGAGGG AGTCCTTCCC AAACCTGCTC CAGAGGAAGA AGAGGAAACC 
TTCATTTTAG ACCTGAAGAA CTTCCCTGAC CTTGCCAATG CAGACCTGGG CTCCCAGAAC 
CCAAACATTC AAGTGACCAT TGAGGTTGTG GACAGCCCAC AGATGGAGAT TGAGATGGAT 
CTGGCCAAGG AGAGCAGGAA CGACTGGTCC TTGAGCTCGG AGGAATGGCT AGGCCATAAA 
AAGCTCTTCT GGCCACTGTT TTGGGAATAC CACGACTCCA GTCAGGAAGG TCCCGGGCAA 
GGCAGTCTGG AGGAGAATGA GGAGGACCTC ATCTATGAAG GAGAAGACTC CTTTTTGAGT 
GGTGTGGGTG CAGACTGGAA CAGTCGATGG AAGGGCTGGG ACTCTACGGA CAATTACGAA 
TATGAGGAGG AAGAATGGAG TGATTGGTCA CCATGTAGTG CCACCTGTGG ACAGGGGAAT 
CAGAAGAGGA TTCGCTCGTG TGGCTATGCC TGCACTGCTA CAGAGTCACG GACATGTGAT 
TTAGAGAGAT GTCCTGATGA CATAATTCCT GTGACGGAAC CAAGTCCTCA TGAAATGGCA 
AACAACACGG AGCTTTTGGG AACAGATGTT GACAGCTGTG AGAAGTGGCT GAACTGCAAG 
AACGACTTCC TCCAGAAATA CCTACACAAA GTCCTGACTG AGTTACCCAA TTGCCCATGT 
GTCTACCCAT CCGAGGTGGT CTACAGTGCT GTCAACGTTT TTGACAGGAA GCTGCAGAAG 
ACCTATCGAT GGCGAGATGC CAGCGGCCCC AAAGAGAGAC TGGACATCTA CAAGCCCTCC 
GCCAGGTTCT GCATTCGCTC CATGCTCTCC TTCGACAGCA CTACTCTGGC GGCACAACAT 
TGCTGCTACG ATGATCACAT GAAGCTCATC ACACGAGGAA AAGGTGCCGG GGCCCCAAAC 
CTCATCAGTA CAGAGTTCTC CCCGGAGCTT CATTACAAAG TGGACGTGCT CCCATGGATC 
CTGTGTAAAG GAGACTGGAG CCGCTTTCAT TCGGTTCGGC CTCCCAATAA CGGTTTACAG 
TGTGTTGACA ACCCACAGGA AAACGTCTAT ATGAATGAGC TAGAAGAGGC CAGAGAGTAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200388.1
CDS	532..1914
Translation	MLIRSQALRWAVFLTLCALMVKGFPAKRRRANHIKSKSGASLEVRGHGGSKDVKQSLQDIQGQNQLRSELPLPRRHKRRWSRIHSEGVLPKPAPEEEEETFILDLKNFPDLANADLGSQNPNIQVTIEVVDSPQMEIEMDLAKESRNDWSLSSEEWLGHKKLFWPLFWEYHDSSQEGPGQGSLEENEEDLIYEGEDSFLSGVGADWNSRWKGWDSTDNYEYEEEEWSDWSPCSATCGQGNQKRIRSCGYACTATESRTCDLERCPDDIIPVTEPSPHEMANNTELLGTDVDSCEKWLNCKNDFLQKYLHKVLTELPNCPCVYPSEVVYSAVNVFDRKLQKTYRWRDASGPKERLDIYKPSARFCIRSMLSFDSTTLAAQHCCYDDHMKLITRGKGAGAPNLISTEFSPELHYKVDVLPWILCKGDWSRFHSVRPPNNGLQCVDNPQENVYMNELEEAREY

Target ORF information:

RefSeq Version	XM_003200340.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio isthmin 2a (ism2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200340.5

ORF Insert Sequence:

ATGCTGATTC GAAGCCAGGC TTTACGGTGG GCAGTTTTCT TGACACTTTG TGCTCTGATG 
GTGAAGGGCT TCCCAGCAAA AAGACGCAGA GCAAATCACA TCAAGAGCAA GAGTGGTGCA 
TCTCTGGAGG TTAGAGGTCA TGGTGGCTCC AAGGATGTCA AGCAGTCTTT GCAAGACATT 
CAGGGTCAAA ACCAACTAAG GAGCGAGTTG CCTTTGCCTC GCCGTCATAA AAGGAGATGG 
TCACGGATTC ACTCGGAGGG AGTCCTTCCC AAACCTGCTC CAGAGGAAGA AGAGGAAACC 
TTCATTTTAG ACCTGAAGAA CTTCCCTGAC CTTGCCAATG CAGACCTGGG CTCCCAGAAC 
CCAAACATTC AAGTGACCAT TGAGGTTGTG GACAGCCCAC AGATGGAGAT TGAGATGGAT 
CTGGCCAAGG AGAGCAGGAA CGACTGGTCC TTGAGCTCGG AGGAATGGCT AGGCCATAAA 
AAGCTCTTCT GGCCACTGTT TTGGGAATAC CACGACTCCA GTCAGGAAGG TCCCGGGCAA 
GGCAGTCTGG AGGAGAATGA GGAGGACCTC ATCTATGAAG GAGAAGACTC CTTTTTGAGT 
GGTGTGGGTG CAGACTGGAA CAGTCGATGG AAGGGCTGGG ACTCTACGGA CAATTACGAA 
TATGAGGAGG AAGAATGGAG TGATTGGTCA CCATGTAGTG CCACCTGTGG ACAGGGGAAT 
CAGAAGAGGA TTCGCTCGTG TGGCTATGCC TGCACTGCTA CAGAGTCACG GACATGTGAT 
TTAGAGAGAT GTCCTGATGA CATAATTCCT GTGACGGAAC CAAGTCCTCA TGAAATGGCA 
AACAACACGG AGCTTTTGGG AACAGATGTT GACAGCTGTG AGAAGTGGCT GAACTGCAAG 
AACGACTTCC TCCAGAAATA CCTACACAAA GTCCTGACTG AGTTACCCAA TTGCCCATGT 
GTCTACCCAT CCGAGGTGGT CTACAGTGCT GTCAACGTTT TTGACAGGAA GCTGCAGAAG 
ACCTATCGAT GGCGAGATGC CAGCGGCCCC AAAGAGAGAC TGGACATCTA CAAGCCCTCC 
GCCAGGTTCT GCATTCGCTC CATGCTCTCC TTCGACAGCA CTACTCTGGC GGCACAACAT 
TGCTGCTACG ATGATCACAT GAAGCTCATC ACACGAGGAA AAGGTGCCGG GGCCCCAAAC 
CTCATCAGTA CAGAGTTCTC CCCGGAGCTT CATTACAAAG TGGACGTGCT CCCATGGATC 
CTGTGTAAAG GAGACTGGAG CCGCTTTCAT TCGGTTCGGC CTCCCAATAA CGGTTTACAG 
TGTGTTGACA ACCCACAGGA AAACGTCTAT ATGAATGAGC TAGAAGAGGC CAGAGAGTAT 
TGA