tmtc3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmtc3
Entrez Gene ID	100150181
Full Name	transmembrane and tetratricopeptide repeat containing 3
General protein information	Preferred Names transmembrane and tetratricopeptide repeat containing 3 Names transmembrane and TPR repeat-containing protein 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001922451.6	XP_001922486.4	transmembrane and TPR repeat-containing protein 3
XM_001922451.7	XP_001922486.4	transmembrane and TPR repeat-containing protein 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TMTC3	XP_532644.1
Danio rerio (zebrafish)	tmtc3	XP_001922486.4
Homo sapiens (human)	TMTC3	NP_861448.2
Bos taurus (cattle)	TMTC3	XP_002687234.2
Rattus norvegicus (Norway rat)	Tmtc3	NP_001129330.1
Drosophila melanogaster (fruit fly)	CG4050	NP_726030.1
Pan troglodytes (chimpanzee)	TMTC3	XP_522486.2
Mus musculus (house mouse)	Tmtc3	NP_001103483.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010599	XP_314563.4
Gallus gallus (chicken)	TMTC3	XP_004937657.1

Gene Ontology

The following tmtc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmtc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa29366	XM_001922451.7 Latest version!	Danio rerio transmembrane and tetratricopeptide repeat containing 3 (tmtc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa29366	XM_001922451.6	Danio rerio transmembrane and tetratricopeptide repeat containing 3 (tmtc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmtc3 ( XM_001922451.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922486.4 Danio rerio transmembrane and tetratricopeptide repeat containing 3 (tmtc3), mRNA.

Price & Availability↑

CloneID	ODa29366
Clone ID Related Accession (Same CDS sequence)	XM_001922451.6 , XM_001922451.7
Accession Version	XM_001922451.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane and TPR repeat-containing protein 3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922451.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAG TGTCGTGGAA GGAGATCTGT CTTCTGACTG GACTGGTGGT GGGCTGCTAC 
TGGAACAGCC TGTTTTGTGG TTTTGTGTTC GATGATGTCT CTGCAATCTT GGATAACAAA 
GACCTTCGTC CCACCACACC GTTAAAGAAC CTCTTCCTCA ATGACTTCTG GGGCACTCCG 
ATGTCAGAGG AGCGGAGCCA TAAGTCCTAC CGACCGCTTA CTGTTCTGAC CTTCCGCCTC 
AATTACCTGT TCAGCGAGTT GAACTCCTCG TCGTACCACC TCCTCAATGT GGTTCTCCAT 
GCTGTGGTGT GTGTCCTCTT TCTCCATTTC TGCCGTCTGC TCCTTGACAG AAGCACCAGC 
CTTATCGCGA CCTTGCTGTT TGCAGTCCAC CCCATCCACA CAGAAGCTGT TACAGGTGTT 
GTTGGCAGGG CTGAGCTGCT ATCCTCCATT TTTCTGCTGG CTGCTTTCCT TGCGTACACC 
AAATCAAAAG GCACCGATCA CTCTATCGTT TGGACTCCTA TCATTTCAAC CGTGTTCCTG 
GTTGCTGTTG CCACGCTATG CAAGGAGCAG GGAATTACTG TCATCGGGAT CTGCTGTATT 
TACGAGGTGT TTGTTGCCCA AGGGTTCACT CTGCCCATGC TGATTGACAC CCTGCTACAG 
GTGTTGAGAG GGAAAGATGG GTTTCCCTAT GCTGTCCTCC AGACCCTGCT GAAGCTCATT 
GTCCTGATCA TCAGTACACT TTTGCTGGTC ATTATTAGGG TTCAGGTCAT TCAGTCCCAG 
CTGCCAGTGT TCACCAGGTT TGATAATCCA GCTGCCGTCA GTTCCACCCC GTCCAGGCAG 
CTGACCTTTA ACTACCTCCT GCCTGTAAAT GCCTGGCTCC TGCTCAACCC CTCAGAGCTT 
TGTTGTGATT GGACGATGGG CACCATCCCT CTCGTGGAGT CCCTGCTGGA CTCACGCAAC 
CTTGCCACAT TGGTGTTTTA CACCTTTTTA GGCCTTCTGG CCTATCACGG TCTTTGGTAC 
AACCACAGCT CCTCAAAGAT CATCATGATG GCACTGTCTC TGATTGTCCT TCCGTTCATC 
CCTGCATCCA ACCTATTTTT CCCTGTGGGC TTCGTTGTTG CTGAAAGGGT CCTATATGTA 
CCCAGCATGG GATTTTGTGT GTTGGTGGCC TACGGATTCA AACTTCTATC CCAAAAAGGG 
GGCCTAAAGA AAATGTCCTG GTTGCTCATG GGAATTCTTC TAACAACGCA TGCTGTAAAA 
ACTTTCAACA GAAACTGGGA CTGGGAGTCC GAATACACGC TTTTTACATC TGCACTGAAA 
GTGAACAGGA ACAATGCCAA ACTGTGGAAC AATGTCGGAC ATGCTTTGGA GAATCAAAAC 
AGCTATGAGA GAGCTTTGAG ATACTTTCTT CAGGCCACAA GAGTGCAACC AGATGACATT 
GGGGCCCACA TGAACGTTGG GAGAACCTAC AAGAACCTAA ACAGGTCCAG AGAGGCAGAG 
GAGGCATATC TAATTGCGAA ATCCCTAATG CCACAGATCA TTCCAGGGAA GAAGTATGCA 
ACTCGTGTGG CCCCCAACCA TCTGAACGTT TACATCAACC TGGCTAACCT AATTCGAGCA 
AATGACTCGC GCCTAGAGGA AGCAGACCAA CTCTATCGAC AGGCCATCAG TATGAGACCA 
GACTTCAAAC AAGCCTACAT CAGCCGAGGG GAGCTTCTGC TGAAAATGAA CAAGCCCAGT 
GAGGCAAAGG ATGCCTACCT ACGAGCACTG GAGCTGGACC ATACCAATGC AGACCTCTGG 
TACAATCTGG CTATAGTCAA TATTGAGATG AAGGACCCCT CAGAGGCTCT GAGAAACTTT 
AATCGCGCAC TGGACCTGAA TCCACGGCAC AAGCTGGCTC TCTTCAACTC AGCGCTTCTC 
ATGCAAGAGT CAGGTGAAGC TAAACTTCGG CCAGAGGCAA ATCGTCGCTT TCTAACCTAC 
GTAAAAGAGG AGCCAGATGA TGCTAATGGC TACTTCAACC TGGGTATGTT AGCGATGGAT 
GCCAACGAGA ACGAAGAGGC CGAGCACTGG ATGCGTGAGG CCATTCATCT GCAACCTGGC 
TTCCGCAGCG CTTTATTTAA CTTGGCCCTG CTCTACTCTC AATCACAGCG TGAGCTGGAT 
GCTCTCCCGG TGCTTGAGGA GCTCCTGCGG TACCACCCAG AGCATGTCAA GGGCCTGATC 
CTCAAGGGAG ATATCCTTAT GAACCACCGC AAAGATACAC GTGGTGCCAA GACCTGTTTT 
GAACGCATCT TGAGCATGGA CCCAAGCAAC GTGCAAGCCA AGCACAACCT ATGTGTGGTG 
TACTTTGAAG AACGGGAGCT CCAGCGTGCA GAACGTTGTC TGGTAGACAC ACTGGCAATG 
GCGCCGCAGG AGGAGTACAT TCGACGACAC CTTGCCATCG TACGCAACAA AATGGCCGCC 
ATGAGTGCCG CAGGGCAGCC GATTGCACCG ACAGAGGGTG GTAGTATGTC AGATAAGCAG 
CAAGGGCATC AACCAGAAGA CAAAAAGGAG GAGGAGGAAG AAGAGGAAGG GCGAGGAGTT 
GCCTCGGGAG CAGGGGAGGG GGCCAGGAAA GAGAAGAATT TGAGGAAATC CTCCTCCACT 
GAAAACCAGG GCCACGGCCA GTCAGAGAAG CTGGACACAG GCCAGCCTGA CAAGCGGACT 
AAGAGCAAAT CCACAAAAGA GATTAAAGAT ATAGAAAAGA AAAGGGCGGC TGCCTTGAAG 
AGACTGGAGG AGATTGAACG CATATTAAGC AGCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922486.4
CDS	281..3079
Translation	MAVVSWKEICLLTGLVVGCYWNSLFCGFVFDDVSAILDNKDLRPTTPLKNLFLNDFWGTPMSEERSHKSYRPLTVLTFRLNYLFSELNSSSYHLLNVVLHAVVCVLFLHFCRLLLDRSTSLIATLLFAVHPIHTEAVTGVVGRAELLSSIFLLAAFLAYTKSKGTDHSIVWTPIISTVFLVAVATLCKEQGITVIGICCIYEVFVAQGFTLPMLIDTLLQVLRGKDGFPYAVLQTLLKLIVLIISTLLLVIIRVQVIQSQLPVFTRFDNPAAVSSTPSRQLTFNYLLPVNAWLLLNPSELCCDWTMGTIPLVESLLDSRNLATLVFYTFLGLLAYHGLWYNHSSSKIIMMALSLIVLPFIPASNLFFPVGFVVAERVLYVPSMGFCVLVAYGFKLLSQKGGLKKMSWLLMGILLTTHAVKTFNRNWDWESEYTLFTSALKVNRNNAKLWNNVGHALENQNSYERALRYFLQATRVQPDDIGAHMNVGRTYKNLNRSREAEEAYLIAKSLMPQIIPGKKYATRVAPNHLNVYINLANLIRANDSRLEEADQLYRQAISMRPDFKQAYISRGELLLKMNKPSEAKDAYLRALELDHTNADLWYNLAIVNIEMKDPSEALRNFNRALDLNPRHKLALFNSALLMQESGEAKLRPEANRRFLTYVKEEPDDANGYFNLGMLAMDANENEEAEHWMREAIHLQPGFRSALFNLALLYSQSQRELDALPVLEELLRYHPEHVKGLILKGDILMNHRKDTRGAKTCFERILSMDPSNVQAKHNLCVVYFEERELQRAERCLVDTLAMAPQEEYIRRHLAIVRNKMAAMSAAGQPIAPTEGGSMSDKQQGHQPEDKKEEEEEEEGRGVASGAGEGARKEKNLRKSSSTENQGHGQSEKLDTGQPDKRTKSKSTKEIKDIEKKRAAALKRLEEIERILSSD

Target ORF information:

RefSeq Version	XM_001922451.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane and tetratricopeptide repeat containing 3 (tmtc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922451.6

ORF Insert Sequence:

ATGGCTGTAG TGTCGTGGAA GGAGATCTGT CTTCTGACTG GACTGGTGGT GGGCTGCTAC 
TGGAACAGCC TGTTTTGTGG TTTTGTGTTC GATGATGTCT CTGCAATCTT GGATAACAAA 
GACCTTCGTC CCACCACACC GTTAAAGAAC CTCTTCCTCA ATGACTTCTG GGGCACTCCG 
ATGTCAGAGG AGCGGAGCCA TAAGTCCTAC CGACCGCTTA CTGTTCTGAC CTTCCGCCTC 
AATTACCTGT TCAGCGAGTT GAACTCCTCG TCGTACCACC TCCTCAATGT GGTTCTCCAT 
GCTGTGGTGT GTGTCCTCTT TCTCCATTTC TGCCGTCTGC TCCTTGACAG AAGCACCAGC 
CTTATCGCGA CCTTGCTGTT TGCAGTCCAC CCCATCCACA CAGAAGCTGT TACAGGTGTT 
GTTGGCAGGG CTGAGCTGCT ATCCTCCATT TTTCTGCTGG CTGCTTTCCT TGCGTACACC 
AAATCAAAAG GCACCGATCA CTCTATCGTT TGGACTCCTA TCATTTCAAC CGTGTTCCTG 
GTTGCTGTTG CCACGCTATG CAAGGAGCAG GGAATTACTG TCATCGGGAT CTGCTGTATT 
TACGAGGTGT TTGTTGCCCA AGGGTTCACT CTGCCCATGC TGATTGACAC CCTGCTACAG 
GTGTTGAGAG GGAAAGATGG GTTTCCCTAT GCTGTCCTCC AGACCCTGCT GAAGCTCATT 
GTCCTGATCA TCAGTACACT TTTGCTGGTC ATTATTAGGG TTCAGGTCAT TCAGTCCCAG 
CTGCCAGTGT TCACCAGGTT TGATAATCCA GCTGCCGTCA GTTCCACCCC GTCCAGGCAG 
CTGACCTTTA ACTACCTCCT GCCTGTAAAT GCCTGGCTCC TGCTCAACCC CTCAGAGCTT 
TGTTGTGATT GGACGATGGG CACCATCCCT CTCGTGGAGT CCCTGCTGGA CTCACGCAAC 
CTTGCCACAT TGGTGTTTTA CACCTTTTTA GGCCTTCTGG CCTATCACGG TCTTTGGTAC 
AACCACAGCT CCTCAAAGAT CATCATGATG GCACTGTCTC TGATTGTCCT TCCGTTCATC 
CCTGCATCCA ACCTATTTTT CCCTGTGGGC TTCGTTGTTG CTGAAAGGGT CCTATATGTA 
CCCAGCATGG GATTTTGTGT GTTGGTGGCC TACGGATTCA AACTTCTATC CCAAAAAGGG 
GGCCTAAAGA AAATGTCCTG GTTGCTCATG GGAATTCTTC TAACAACGCA TGCTGTAAAA 
ACTTTCAACA GAAACTGGGA CTGGGAGTCC GAATACACGC TTTTTACATC TGCACTGAAA 
GTGAACAGGA ACAATGCCAA ACTGTGGAAC AATGTCGGAC ATGCTTTGGA GAATCAAAAC 
AGCTATGAGA GAGCTTTGAG ATACTTTCTT CAGGCCACAA GAGTGCAACC AGATGACATT 
GGGGCCCACA TGAACGTTGG GAGAACCTAC AAGAACCTAA ACAGGTCCAG AGAGGCAGAG 
GAGGCATATC TAATTGCGAA ATCCCTAATG CCACAGATCA TTCCAGGGAA GAAGTATGCA 
ACTCGTGTGG CCCCCAACCA TCTGAACGTT TACATCAACC TGGCTAACCT AATTCGAGCA 
AATGACTCGC GCCTAGAGGA AGCAGACCAA CTCTATCGAC AGGCCATCAG TATGAGACCA 
GACTTCAAAC AAGCCTACAT CAGCCGAGGG GAGCTTCTGC TGAAAATGAA CAAGCCCAGT 
GAGGCAAAGG ATGCCTACCT ACGAGCACTG GAGCTGGACC ATACCAATGC AGACCTCTGG 
TACAATCTGG CTATAGTCAA TATTGAGATG AAGGACCCCT CAGAGGCTCT GAGAAACTTT 
AATCGCGCAC TGGACCTGAA TCCACGGCAC AAGCTGGCTC TCTTCAACTC AGCGCTTCTC 
ATGCAAGAGT CAGGTGAAGC TAAACTTCGG CCAGAGGCAA ATCGTCGCTT TCTAACCTAC 
GTAAAAGAGG AGCCAGATGA TGCTAATGGC TACTTCAACC TGGGTATGTT AGCGATGGAT 
GCCAACGAGA ACGAAGAGGC CGAGCACTGG ATGCGTGAGG CCATTCATCT GCAACCTGGC 
TTCCGCAGCG CTTTATTTAA CTTGGCCCTG CTCTACTCTC AATCACAGCG TGAGCTGGAT 
GCTCTCCCGG TGCTTGAGGA GCTCCTGCGG TACCACCCAG AGCATGTCAA GGGCCTGATC 
CTCAAGGGAG ATATCCTTAT GAACCACCGC AAAGATACAC GTGGTGCCAA GACCTGTTTT 
GAACGCATCT TGAGCATGGA CCCAAGCAAC GTGCAAGCCA AGCACAACCT ATGTGTGGTG 
TACTTTGAAG AACGGGAGCT CCAGCGTGCA GAACGTTGTC TGGTAGACAC ACTGGCAATG 
GCGCCGCAGG AGGAGTACAT TCGACGACAC CTTGCCATCG TACGCAACAA AATGGCCGCC 
ATGAGTGCCG CAGGGCAGCC GATTGCACCG ACAGAGGGTG GTAGTATGTC AGATAAGCAG 
CAAGGGCATC AACCAGAAGA CAAAAAGGAG GAGGAGGAAG AAGAGGAAGG GCGAGGAGTT 
GCCTCGGGAG CAGGGGAGGG GGCCAGGAAA GAGAAGAATT TGAGGAAATC CTCCTCCACT 
GAAAACCAGG GCCACGGCCA GTCAGAGAAG CTGGACACAG GCCAGCCTGA CAAGCGGACT 
AAGAGCAAAT CCACAAAAGA GATTAAAGAT ATAGAAAAGA AAAGGGCGGC TGCCTTGAAG 
AGACTGGAGG AGATTGAACG CATATTAAGC AGCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmtc3 ( XM_001922451.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922486.4 Danio rerio transmembrane and tetratricopeptide repeat containing 3 (tmtc3), mRNA.

Price & Availability↑

CloneID	ODa29366
Clone ID Related Accession (Same CDS sequence)	XM_001922451.6 , XM_001922451.7
Accession Version	XM_001922451.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane and TPR repeat-containing protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922451.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAG TGTCGTGGAA GGAGATCTGT CTTCTGACTG GACTGGTGGT GGGCTGCTAC 
TGGAACAGCC TGTTTTGTGG TTTTGTGTTC GATGATGTCT CTGCAATCTT GGATAACAAA 
GACCTTCGTC CCACCACACC GTTAAAGAAC CTCTTCCTCA ATGACTTCTG GGGCACTCCG 
ATGTCAGAGG AGCGGAGCCA TAAGTCCTAC CGACCGCTTA CTGTTCTGAC CTTCCGCCTC 
AATTACCTGT TCAGCGAGTT GAACTCCTCG TCGTACCACC TCCTCAATGT GGTTCTCCAT 
GCTGTGGTGT GTGTCCTCTT TCTCCATTTC TGCCGTCTGC TCCTTGACAG AAGCACCAGC 
CTTATCGCGA CCTTGCTGTT TGCAGTCCAC CCCATCCACA CAGAAGCTGT TACAGGTGTT 
GTTGGCAGGG CTGAGCTGCT ATCCTCCATT TTTCTGCTGG CTGCTTTCCT TGCGTACACC 
AAATCAAAAG GCACCGATCA CTCTATCGTT TGGACTCCTA TCATTTCAAC CGTGTTCCTG 
GTTGCTGTTG CCACGCTATG CAAGGAGCAG GGAATTACTG TCATCGGGAT CTGCTGTATT 
TACGAGGTGT TTGTTGCCCA AGGGTTCACT CTGCCCATGC TGATTGACAC CCTGCTACAG 
GTGTTGAGAG GGAAAGATGG GTTTCCCTAT GCTGTCCTCC AGACCCTGCT GAAGCTCATT 
GTCCTGATCA TCAGTACACT TTTGCTGGTC ATTATTAGGG TTCAGGTCAT TCAGTCCCAG 
CTGCCAGTGT TCACCAGGTT TGATAATCCA GCTGCCGTCA GTTCCACCCC GTCCAGGCAG 
CTGACCTTTA ACTACCTCCT GCCTGTAAAT GCCTGGCTCC TGCTCAACCC CTCAGAGCTT 
TGTTGTGATT GGACGATGGG CACCATCCCT CTCGTGGAGT CCCTGCTGGA CTCACGCAAC 
CTTGCCACAT TGGTGTTTTA CACCTTTTTA GGCCTTCTGG CCTATCACGG TCTTTGGTAC 
AACCACAGCT CCTCAAAGAT CATCATGATG GCACTGTCTC TGATTGTCCT TCCGTTCATC 
CCTGCATCCA ACCTATTTTT CCCTGTGGGC TTCGTTGTTG CTGAAAGGGT CCTATATGTA 
CCCAGCATGG GATTTTGTGT GTTGGTGGCC TACGGATTCA AACTTCTATC CCAAAAAGGG 
GGCCTAAAGA AAATGTCCTG GTTGCTCATG GGAATTCTTC TAACAACGCA TGCTGTAAAA 
ACTTTCAACA GAAACTGGGA CTGGGAGTCC GAATACACGC TTTTTACATC TGCACTGAAA 
GTGAACAGGA ACAATGCCAA ACTGTGGAAC AATGTCGGAC ATGCTTTGGA GAATCAAAAC 
AGCTATGAGA GAGCTTTGAG ATACTTTCTT CAGGCCACAA GAGTGCAACC AGATGACATT 
GGGGCCCACA TGAACGTTGG GAGAACCTAC AAGAACCTAA ACAGGTCCAG AGAGGCAGAG 
GAGGCATATC TAATTGCGAA ATCCCTAATG CCACAGATCA TTCCAGGGAA GAAGTATGCA 
ACTCGTGTGG CCCCCAACCA TCTGAACGTT TACATCAACC TGGCTAACCT AATTCGAGCA 
AATGACTCGC GCCTAGAGGA AGCAGACCAA CTCTATCGAC AGGCCATCAG TATGAGACCA 
GACTTCAAAC AAGCCTACAT CAGCCGAGGG GAGCTTCTGC TGAAAATGAA CAAGCCCAGT 
GAGGCAAAGG ATGCCTACCT ACGAGCACTG GAGCTGGACC ATACCAATGC AGACCTCTGG 
TACAATCTGG CTATAGTCAA TATTGAGATG AAGGACCCCT CAGAGGCTCT GAGAAACTTT 
AATCGCGCAC TGGACCTGAA TCCACGGCAC AAGCTGGCTC TCTTCAACTC AGCGCTTCTC 
ATGCAAGAGT CAGGTGAAGC TAAACTTCGG CCAGAGGCAA ATCGTCGCTT TCTAACCTAC 
GTAAAAGAGG AGCCAGATGA TGCTAATGGC TACTTCAACC TGGGTATGTT AGCGATGGAT 
GCCAACGAGA ACGAAGAGGC CGAGCACTGG ATGCGTGAGG CCATTCATCT GCAACCTGGC 
TTCCGCAGCG CTTTATTTAA CTTGGCCCTG CTCTACTCTC AATCACAGCG TGAGCTGGAT 
GCTCTCCCGG TGCTTGAGGA GCTCCTGCGG TACCACCCAG AGCATGTCAA GGGCCTGATC 
CTCAAGGGAG ATATCCTTAT GAACCACCGC AAAGATACAC GTGGTGCCAA GACCTGTTTT 
GAACGCATCT TGAGCATGGA CCCAAGCAAC GTGCAAGCCA AGCACAACCT ATGTGTGGTG 
TACTTTGAAG AACGGGAGCT CCAGCGTGCA GAACGTTGTC TGGTAGACAC ACTGGCAATG 
GCGCCGCAGG AGGAGTACAT TCGACGACAC CTTGCCATCG TACGCAACAA AATGGCCGCC 
ATGAGTGCCG CAGGGCAGCC GATTGCACCG ACAGAGGGTG GTAGTATGTC AGATAAGCAG 
CAAGGGCATC AACCAGAAGA CAAAAAGGAG GAGGAGGAAG AAGAGGAAGG GCGAGGAGTT 
GCCTCGGGAG CAGGGGAGGG GGCCAGGAAA GAGAAGAATT TGAGGAAATC CTCCTCCACT 
GAAAACCAGG GCCACGGCCA GTCAGAGAAG CTGGACACAG GCCAGCCTGA CAAGCGGACT 
AAGAGCAAAT CCACAAAAGA GATTAAAGAT ATAGAAAAGA AAAGGGCGGC TGCCTTGAAG 
AGACTGGAGG AGATTGAACG CATATTAAGC AGCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922486.4
CDS	281..3079
Translation	MAVVSWKEICLLTGLVVGCYWNSLFCGFVFDDVSAILDNKDLRPTTPLKNLFLNDFWGTPMSEERSHKSYRPLTVLTFRLNYLFSELNSSSYHLLNVVLHAVVCVLFLHFCRLLLDRSTSLIATLLFAVHPIHTEAVTGVVGRAELLSSIFLLAAFLAYTKSKGTDHSIVWTPIISTVFLVAVATLCKEQGITVIGICCIYEVFVAQGFTLPMLIDTLLQVLRGKDGFPYAVLQTLLKLIVLIISTLLLVIIRVQVIQSQLPVFTRFDNPAAVSSTPSRQLTFNYLLPVNAWLLLNPSELCCDWTMGTIPLVESLLDSRNLATLVFYTFLGLLAYHGLWYNHSSSKIIMMALSLIVLPFIPASNLFFPVGFVVAERVLYVPSMGFCVLVAYGFKLLSQKGGLKKMSWLLMGILLTTHAVKTFNRNWDWESEYTLFTSALKVNRNNAKLWNNVGHALENQNSYERALRYFLQATRVQPDDIGAHMNVGRTYKNLNRSREAEEAYLIAKSLMPQIIPGKKYATRVAPNHLNVYINLANLIRANDSRLEEADQLYRQAISMRPDFKQAYISRGELLLKMNKPSEAKDAYLRALELDHTNADLWYNLAIVNIEMKDPSEALRNFNRALDLNPRHKLALFNSALLMQESGEAKLRPEANRRFLTYVKEEPDDANGYFNLGMLAMDANENEEAEHWMREAIHLQPGFRSALFNLALLYSQSQRELDALPVLEELLRYHPEHVKGLILKGDILMNHRKDTRGAKTCFERILSMDPSNVQAKHNLCVVYFEERELQRAERCLVDTLAMAPQEEYIRRHLAIVRNKMAAMSAAGQPIAPTEGGSMSDKQQGHQPEDKKEEEEEEEGRGVASGAGEGARKEKNLRKSSSTENQGHGQSEKLDTGQPDKRTKSKSTKEIKDIEKKRAAALKRLEEIERILSSD

Target ORF information:

RefSeq Version	XM_001922451.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane and tetratricopeptide repeat containing 3 (tmtc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922451.7

ORF Insert Sequence:

ATGGCTGTAG TGTCGTGGAA GGAGATCTGT CTTCTGACTG GACTGGTGGT GGGCTGCTAC 
TGGAACAGCC TGTTTTGTGG TTTTGTGTTC GATGATGTCT CTGCAATCTT GGATAACAAA 
GACCTTCGTC CCACCACACC GTTAAAGAAC CTCTTCCTCA ATGACTTCTG GGGCACTCCG 
ATGTCAGAGG AGCGGAGCCA TAAGTCCTAC CGACCGCTTA CTGTTCTGAC CTTCCGCCTC 
AATTACCTGT TCAGCGAGTT GAACTCCTCG TCGTACCACC TCCTCAATGT GGTTCTCCAT 
GCTGTGGTGT GTGTCCTCTT TCTCCATTTC TGCCGTCTGC TCCTTGACAG AAGCACCAGC 
CTTATCGCGA CCTTGCTGTT TGCAGTCCAC CCCATCCACA CAGAAGCTGT TACAGGTGTT 
GTTGGCAGGG CTGAGCTGCT ATCCTCCATT TTTCTGCTGG CTGCTTTCCT TGCGTACACC 
AAATCAAAAG GCACCGATCA CTCTATCGTT TGGACTCCTA TCATTTCAAC CGTGTTCCTG 
GTTGCTGTTG CCACGCTATG CAAGGAGCAG GGAATTACTG TCATCGGGAT CTGCTGTATT 
TACGAGGTGT TTGTTGCCCA AGGGTTCACT CTGCCCATGC TGATTGACAC CCTGCTACAG 
GTGTTGAGAG GGAAAGATGG GTTTCCCTAT GCTGTCCTCC AGACCCTGCT GAAGCTCATT 
GTCCTGATCA TCAGTACACT TTTGCTGGTC ATTATTAGGG TTCAGGTCAT TCAGTCCCAG 
CTGCCAGTGT TCACCAGGTT TGATAATCCA GCTGCCGTCA GTTCCACCCC GTCCAGGCAG 
CTGACCTTTA ACTACCTCCT GCCTGTAAAT GCCTGGCTCC TGCTCAACCC CTCAGAGCTT 
TGTTGTGATT GGACGATGGG CACCATCCCT CTCGTGGAGT CCCTGCTGGA CTCACGCAAC 
CTTGCCACAT TGGTGTTTTA CACCTTTTTA GGCCTTCTGG CCTATCACGG TCTTTGGTAC 
AACCACAGCT CCTCAAAGAT CATCATGATG GCACTGTCTC TGATTGTCCT TCCGTTCATC 
CCTGCATCCA ACCTATTTTT CCCTGTGGGC TTCGTTGTTG CTGAAAGGGT CCTATATGTA 
CCCAGCATGG GATTTTGTGT GTTGGTGGCC TACGGATTCA AACTTCTATC CCAAAAAGGG 
GGCCTAAAGA AAATGTCCTG GTTGCTCATG GGAATTCTTC TAACAACGCA TGCTGTAAAA 
ACTTTCAACA GAAACTGGGA CTGGGAGTCC GAATACACGC TTTTTACATC TGCACTGAAA 
GTGAACAGGA ACAATGCCAA ACTGTGGAAC AATGTCGGAC ATGCTTTGGA GAATCAAAAC 
AGCTATGAGA GAGCTTTGAG ATACTTTCTT CAGGCCACAA GAGTGCAACC AGATGACATT 
GGGGCCCACA TGAACGTTGG GAGAACCTAC AAGAACCTAA ACAGGTCCAG AGAGGCAGAG 
GAGGCATATC TAATTGCGAA ATCCCTAATG CCACAGATCA TTCCAGGGAA GAAGTATGCA 
ACTCGTGTGG CCCCCAACCA TCTGAACGTT TACATCAACC TGGCTAACCT AATTCGAGCA 
AATGACTCGC GCCTAGAGGA AGCAGACCAA CTCTATCGAC AGGCCATCAG TATGAGACCA 
GACTTCAAAC AAGCCTACAT CAGCCGAGGG GAGCTTCTGC TGAAAATGAA CAAGCCCAGT 
GAGGCAAAGG ATGCCTACCT ACGAGCACTG GAGCTGGACC ATACCAATGC AGACCTCTGG 
TACAATCTGG CTATAGTCAA TATTGAGATG AAGGACCCCT CAGAGGCTCT GAGAAACTTT 
AATCGCGCAC TGGACCTGAA TCCACGGCAC AAGCTGGCTC TCTTCAACTC AGCGCTTCTC 
ATGCAAGAGT CAGGTGAAGC TAAACTTCGG CCAGAGGCAA ATCGTCGCTT TCTAACCTAC 
GTAAAAGAGG AGCCAGATGA TGCTAATGGC TACTTCAACC TGGGTATGTT AGCGATGGAT 
GCCAACGAGA ACGAAGAGGC CGAGCACTGG ATGCGTGAGG CCATTCATCT GCAACCTGGC 
TTCCGCAGCG CTTTATTTAA CTTGGCCCTG CTCTACTCTC AATCACAGCG TGAGCTGGAT 
GCTCTCCCGG TGCTTGAGGA GCTCCTGCGG TACCACCCAG AGCATGTCAA GGGCCTGATC 
CTCAAGGGAG ATATCCTTAT GAACCACCGC AAAGATACAC GTGGTGCCAA GACCTGTTTT 
GAACGCATCT TGAGCATGGA CCCAAGCAAC GTGCAAGCCA AGCACAACCT ATGTGTGGTG 
TACTTTGAAG AACGGGAGCT CCAGCGTGCA GAACGTTGTC TGGTAGACAC ACTGGCAATG 
GCGCCGCAGG AGGAGTACAT TCGACGACAC CTTGCCATCG TACGCAACAA AATGGCCGCC 
ATGAGTGCCG CAGGGCAGCC GATTGCACCG ACAGAGGGTG GTAGTATGTC AGATAAGCAG 
CAAGGGCATC AACCAGAAGA CAAAAAGGAG GAGGAGGAAG AAGAGGAAGG GCGAGGAGTT 
GCCTCGGGAG CAGGGGAGGG GGCCAGGAAA GAGAAGAATT TGAGGAAATC CTCCTCCACT 
GAAAACCAGG GCCACGGCCA GTCAGAGAAG CTGGACACAG GCCAGCCTGA CAAGCGGACT 
AAGAGCAAAT CCACAAAAGA GATTAAAGAT ATAGAAAAGA AAAGGGCGGC TGCCTTGAAG 
AGACTGGAGG AGATTGAACG CATATTAAGC AGCGATTAA