mthfd1l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mthfd1l
Entrez Gene ID	100034522
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
Synonyms	fk48e08,si:dkey-25e12.5,si:dkey-25e12.6,wu:fk48e08
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like Names monofunctional C1-tetrahydrofolate synthase, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160551.3	XP_005160608.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
NM_001242996.1	NP_001229925.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial
XM_005160551.4	XP_005160608.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
XM_005160552.3	XP_005160609.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
XM_009299369.2	XP_009297644.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X2
XM_005160552.4	XP_005160609.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MTHFD1L	XP_518807.3
	MTHFD1L	XP_001098522.2
Danio rerio (zebrafish)	mthfd1l	NP_001229925.1
Homo sapiens (human)	MTHFD1L	NP_001229696.1
Rattus norvegicus (Norway rat)	Mthfd1l	NP_001101932.2
Canis lupus familiaris (dog)	MTHFD1L	XP_533450.2
Bos taurus (cattle)	MTHFD1L	NP_001069486.1
Gallus gallus (chicken)	MTHFD1L	XP_004935675.1
Mus musculus (house mouse)	Mthfd1l	NP_758512.3
Xenopus tropicalis (tropical clawed frog)	mthfd1l	NP_001120171.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Disruption of the folate pathway in zebrafish causes developmental defects.
Lee MS, Bonner JR, Bernard DJ, Sanchez EL, Sause ET, Prentice RR, Burgess SM, Brody LC
BMC developmental biology1212(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mthfd1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mthfd1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24836	NM_001242996.1 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (mthfd1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa24836	XM_005160552.4 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa24836	XM_005160551.4 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa48384	XM_009299369.2 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa24836	XM_005160551.3	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa24836	XM_005160552.3	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mthfd1l ( NM_001242996.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001229925.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (mthfd1l), mRNA.

Price & Availability↑

CloneID	ODa24836
Clone ID Related Accession (Same CDS sequence)	XM_005160551.3 , NM_001242996.1 , XM_005160551.4 , XM_005160552.3 , XM_005160552.4
Accession Version	NM_001242996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX537272.5. On Jul 14, 2011 this sequence version replaced XM_003200705.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GFIL01027079.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229925.1
CDS	66..3002
Translation	MMRLSVAFRHARRSHSTIQRSRTCVKFLSRENRHTPDISAVSFLRAREASSTSGTTETAASLQSHARKYEGPPYERAVGDIVQRCREELKSIGENNPGLKPTLAIIQAGEDDGLLEMNKKMAGKIGLNVMQICLPHRCTEEEVIEEVLRLNKDSRVHGIFLHLPQTFLSKSVRNAIKPQKDVDGVCDLNVGRVVLGDKTDGFTSPVAGAVLELLAKHDAPLVGKMAVLVGLEGPLKVALQCLLQNNGMILQTCRWSSTNLQKQIMDSDVVITLNTNQNHIPSTWLRPGVAAINLGSALLEEHPDSWEATVEAQSAAGLGPLTAAFRMQHVVRSSRRWIQEQQYQPWNIRPLKLHPLSPVPSDIEISRAQTPKPISQLAQEIGLLPEELEAYGNTKAKVHLSLLNRLQHQPDGRYVLVAGITPTPLGEGKSTVTIGLAQALTAHLKLNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARILHEATQSDKALYSRLVPSVNGVRRFSPIQLARLQRLGINKSNPTDLTPQEVSAFVRLDLDPEKVTWQRVVDTNDRFLRKITIGQANTEKGHARQTQFDIAVASEIMAILALTDGLADMRARLGRMVVGSSRNGQAITADDLGVTGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGNSSVLADKLALKLVGEEGYVVTEAGFGADIGMEKFFNIKCRTSGLRPNVVVLVATIRALKMHGGGPNVTAGAPLPKEYIEENLQLVADGCSNLKKQIQIAHLFGVPVVVALNAFKTDTQAEIDLVCRIAKASGASAAVPCHHWSRGGRGSLELAHAVKEVAAQGSRFQFLYNLQDPIVEKIRTIAQKVYGAEDIELSSEAQAKIDYYNQQGFGALPICMAKTHLSLSHMPEKKGVPTNFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDPVTEEIKGLF

Target ORF information:

RefSeq Version	NM_001242996.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (mthfd1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242996.1

ORF Insert Sequence:

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mthfd1l ( XM_005160551.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160608.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24836
Clone ID Related Accession (Same CDS sequence)	XM_005160551.3 , NM_001242996.1 , XM_005160551.4 , XM_005160552.3 , XM_005160552.4
Accession Version	XM_005160551.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160551.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160608.1
CDS	1..2937
Translation	MMRLSVAFRHARRSHSTIQRSRTCVKFLSRENRHTPDISAVSFLRAREASSTSGTTETAASLQSHARKYEGPPYERAVGDIVQRCREELKSIGENNPGLKPTLAIIQAGEDDGLLEMNKKMAGKIGLNVMQICLPHRCTEEEVIEEVLRLNKDSRVHGIFLHLPQTFLSKSVRNAIKPQKDVDGVCDLNVGRVVLGDKTDGFTSPVAGAVLELLAKHDAPLVGKMAVLVGLEGPLKVALQCLLQNNGMILQTCRWSSTNLQKQIMDSDVVITLNTNQNHIPSTWLRPGVAAINLGSALLEEHPDSWEATVEAQSAAGLGPLTAAFRMQHVVRSSRRWIQEQQYQPWNIRPLKLHPLSPVPSDIEISRAQTPKPISQLAQEIGLLPEELEAYGNTKAKVHLSLLNRLQHQPDGRYVLVAGITPTPLGEGKSTVTIGLAQALTAHLKLNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARILHEATQSDKALYSRLVPSVNGVRRFSPIQLARLQRLGINKSNPTDLTPQEVSAFVRLDLDPEKVTWQRVVDTNDRFLRKITIGQANTEKGHARQTQFDIAVASEIMAILALTDGLADMRARLGRMVVGSSRNGQAITADDLGVTGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGNSSVLADKLALKLVGEEGYVVTEAGFGADIGMEKFFNIKCRTSGLRPNVVVLVATIRALKMHGGGPNVTAGAPLPKEYIEENLQLVADGCSNLKKQIQIAHLFGVPVVVALNAFKTDTQAEIDLVCRIAKASGASAAVPCHHWSRGGRGSLELAHAVKEVAAQGSRFQFLYNLQDPIVEKIRTIAQKVYGAEDIELSSEAQAKIDYYNQQGFGALPICMAKTHLSLSHMPEKKGVPTNFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDPVTEEIKGLF

Target ORF information:

RefSeq Version	XM_005160551.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160551.3

ORF Insert Sequence:

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mthfd1l ( XM_005160551.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160608.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24836
Clone ID Related Accession (Same CDS sequence)	XM_005160551.3 , NM_001242996.1 , XM_005160551.4 , XM_005160552.3 , XM_005160552.4
Accession Version	XM_005160551.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160551.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160608.1
CDS	1..2937
Translation	MMRLSVAFRHARRSHSTIQRSRTCVKFLSRENRHTPDISAVSFLRAREASSTSGTTETAASLQSHARKYEGPPYERAVGDIVQRCREELKSIGENNPGLKPTLAIIQAGEDDGLLEMNKKMAGKIGLNVMQICLPHRCTEEEVIEEVLRLNKDSRVHGIFLHLPQTFLSKSVRNAIKPQKDVDGVCDLNVGRVVLGDKTDGFTSPVAGAVLELLAKHDAPLVGKMAVLVGLEGPLKVALQCLLQNNGMILQTCRWSSTNLQKQIMDSDVVITLNTNQNHIPSTWLRPGVAAINLGSALLEEHPDSWEATVEAQSAAGLGPLTAAFRMQHVVRSSRRWIQEQQYQPWNIRPLKLHPLSPVPSDIEISRAQTPKPISQLAQEIGLLPEELEAYGNTKAKVHLSLLNRLQHQPDGRYVLVAGITPTPLGEGKSTVTIGLAQALTAHLKLNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARILHEATQSDKALYSRLVPSVNGVRRFSPIQLARLQRLGINKSNPTDLTPQEVSAFVRLDLDPEKVTWQRVVDTNDRFLRKITIGQANTEKGHARQTQFDIAVASEIMAILALTDGLADMRARLGRMVVGSSRNGQAITADDLGVTGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGNSSVLADKLALKLVGEEGYVVTEAGFGADIGMEKFFNIKCRTSGLRPNVVVLVATIRALKMHGGGPNVTAGAPLPKEYIEENLQLVADGCSNLKKQIQIAHLFGVPVVVALNAFKTDTQAEIDLVCRIAKASGASAAVPCHHWSRGGRGSLELAHAVKEVAAQGSRFQFLYNLQDPIVEKIRTIAQKVYGAEDIELSSEAQAKIDYYNQQGFGALPICMAKTHLSLSHMPEKKGVPTNFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDPVTEEIKGLF

Target ORF information:

RefSeq Version	XM_005160551.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160551.4

ORF Insert Sequence:

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mthfd1l ( XM_005160552.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160609.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24836
Clone ID Related Accession (Same CDS sequence)	XM_005160551.3 , NM_001242996.1 , XM_005160551.4 , XM_005160552.3 , XM_005160552.4
Accession Version	XM_005160552.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160609.1
CDS	115..3051
Translation	MMRLSVAFRHARRSHSTIQRSRTCVKFLSRENRHTPDISAVSFLRAREASSTSGTTETAASLQSHARKYEGPPYERAVGDIVQRCREELKSIGENNPGLKPTLAIIQAGEDDGLLEMNKKMAGKIGLNVMQICLPHRCTEEEVIEEVLRLNKDSRVHGIFLHLPQTFLSKSVRNAIKPQKDVDGVCDLNVGRVVLGDKTDGFTSPVAGAVLELLAKHDAPLVGKMAVLVGLEGPLKVALQCLLQNNGMILQTCRWSSTNLQKQIMDSDVVITLNTNQNHIPSTWLRPGVAAINLGSALLEEHPDSWEATVEAQSAAGLGPLTAAFRMQHVVRSSRRWIQEQQYQPWNIRPLKLHPLSPVPSDIEISRAQTPKPISQLAQEIGLLPEELEAYGNTKAKVHLSLLNRLQHQPDGRYVLVAGITPTPLGEGKSTVTIGLAQALTAHLKLNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARILHEATQSDKALYSRLVPSVNGVRRFSPIQLARLQRLGINKSNPTDLTPQEVSAFVRLDLDPEKVTWQRVVDTNDRFLRKITIGQANTEKGHARQTQFDIAVASEIMAILALTDGLADMRARLGRMVVGSSRNGQAITADDLGVTGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGNSSVLADKLALKLVGEEGYVVTEAGFGADIGMEKFFNIKCRTSGLRPNVVVLVATIRALKMHGGGPNVTAGAPLPKEYIEENLQLVADGCSNLKKQIQIAHLFGVPVVVALNAFKTDTQAEIDLVCRIAKASGASAAVPCHHWSRGGRGSLELAHAVKEVAAQGSRFQFLYNLQDPIVEKIRTIAQKVYGAEDIELSSEAQAKIDYYNQQGFGALPICMAKTHLSLSHMPEKKGVPTNFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDPVTEEIKGLF

Target ORF information:

RefSeq Version	XM_005160552.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160552.3

ORF Insert Sequence:

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mthfd1l ( XM_005160552.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160609.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24836
Clone ID Related Accession (Same CDS sequence)	XM_005160551.3 , NM_001242996.1 , XM_005160551.4 , XM_005160552.3 , XM_005160552.4
Accession Version	XM_005160552.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160552.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160609.1
CDS	282..3218
Translation	MMRLSVAFRHARRSHSTIQRSRTCVKFLSRENRHTPDISAVSFLRAREASSTSGTTETAASLQSHARKYEGPPYERAVGDIVQRCREELKSIGENNPGLKPTLAIIQAGEDDGLLEMNKKMAGKIGLNVMQICLPHRCTEEEVIEEVLRLNKDSRVHGIFLHLPQTFLSKSVRNAIKPQKDVDGVCDLNVGRVVLGDKTDGFTSPVAGAVLELLAKHDAPLVGKMAVLVGLEGPLKVALQCLLQNNGMILQTCRWSSTNLQKQIMDSDVVITLNTNQNHIPSTWLRPGVAAINLGSALLEEHPDSWEATVEAQSAAGLGPLTAAFRMQHVVRSSRRWIQEQQYQPWNIRPLKLHPLSPVPSDIEISRAQTPKPISQLAQEIGLLPEELEAYGNTKAKVHLSLLNRLQHQPDGRYVLVAGITPTPLGEGKSTVTIGLAQALTAHLKLNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARILHEATQSDKALYSRLVPSVNGVRRFSPIQLARLQRLGINKSNPTDLTPQEVSAFVRLDLDPEKVTWQRVVDTNDRFLRKITIGQANTEKGHARQTQFDIAVASEIMAILALTDGLADMRARLGRMVVGSSRNGQAITADDLGVTGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGNSSVLADKLALKLVGEEGYVVTEAGFGADIGMEKFFNIKCRTSGLRPNVVVLVATIRALKMHGGGPNVTAGAPLPKEYIEENLQLVADGCSNLKKQIQIAHLFGVPVVVALNAFKTDTQAEIDLVCRIAKASGASAAVPCHHWSRGGRGSLELAHAVKEVAAQGSRFQFLYNLQDPIVEKIRTIAQKVYGAEDIELSSEAQAKIDYYNQQGFGALPICMAKTHLSLSHMPEKKGVPTNFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDPVTEEIKGLF

Target ORF information:

RefSeq Version	XM_005160552.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160552.4

ORF Insert Sequence:

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGTGACA GAAGCTGGTT TTGGTGCAGA TATTGGCATG 
GAGAAGTTTT TCAACATTAA GTGTCGGACT TCTGGACTGA GGCCTAATGT TGTTGTGCTG 
GTTGCCACCA TACGCGCTCT GAAGATGCAT GGAGGTGGAC CAAATGTTAC AGCTGGTGCA 
CCACTGCCTA AAGAATATAT CGAGGAGAAT TTACAGCTGG TTGCAGATGG CTGCAGTAAC 
CTGAAGAAGC AGATTCAGAT CGCTCATCTC TTTGGAGTTC CTGTGGTTGT TGCTTTAAAT 
GCCTTCAAGA CAGACACTCA AGCAGAGATT GACCTGGTTT GTCGCATTGC AAAGGCTTCT 
GGGGCGTCCG CTGCAGTGCC ATGTCATCAT TGGTCCAGAG GTGGACGGGG CTCTCTTGAA 
CTGGCACACG CTGTAAAGGA AGTTGCTGCT CAGGGAAGCC GTTTTCAGTT CCTGTATAAC 
TTGCAGGATC CTATTGTAGA GAAAATTCGC ACTATTGCAC AGAAGGTTTA CGGCGCTGAA 
GACATTGAGC TTTCATCTGA GGCTCAAGCT AAGATTGACT ACTACAATCA ACAGGGCTTT 
GGTGCTCTTC CTATCTGCAT GGCCAAAACC CACCTGTCTC TCTCTCACAT GCCTGAGAAA 
AAAGGTGTTC CCACCAACTT TATCCTGCCC ATCAGAGACG TCCGTGCCAG CATCGGCGCA 
GGCTTTATCT ACCCTTTAGT TGGAACAATG AGCACTATGC CAGGCTTGCC TACACGACCC 
TGTTTCTATG ACATTGACCT TGATCCTGTG ACCGAGGAGA TAAAAGGCCT TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mthfd1l ( XM_009299369.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297644.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa48384
Clone ID Related Accession (Same CDS sequence)	XM_009299369.2
Accession Version	XM_009299369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGCGATG TGCTCCAGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297644.1
CDS	115..2259
Translation	MMRLSVAFRHARRSHSTIQRSRTCVKFLSRENRHTPDISAVSFLRAREASSTSGTTETAASLQSHARKYEGPPYERAVGDIVQRCREELKSIGENNPGLKPTLAIIQAGEDDGLLEMNKKMAGKIGLNVMQICLPHRCTEEEVIEEVLRLNKDSRVHGIFLHLPQTFLSKSVRNAIKPQKDVDGVCDLNVGRVVLGDKTDGFTSPVAGAVLELLAKHDAPLVGKMAVLVGLEGPLKVALQCLLQNNGMILQTCRWSSTNLQKQIMDSDVVITLNTNQNHIPSTWLRPGVAAINLGSALLEEHPDSWEATVEAQSAAGLGPLTAAFRMQHVVRSSRRWIQEQQYQPWNIRPLKLHPLSPVPSDIEISRAQTPKPISQLAQEIGLLPEELEAYGNTKAKVHLSLLNRLQHQPDGRYVLVAGITPTPLGEGKSTVTIGLAQALTAHLKLNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARILHEATQSDKALYSRLVPSVNGVRRFSPIQLARLQRLGINKSNPTDLTPQEVSAFVRLDLDPEKVTWQRVVDTNDRFLRKITIGQANTEKGHARQTQFDIAVASEIMAILALTDGLADMRARLGRMVVGSSRNGQAITADDLGVTGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGNSSVLADKLALKLVGEEGYVAMCSRH

Target ORF information:

RefSeq Version	XM_009299369.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (mthfd1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299369.2

ORF Insert Sequence:

ATGATGAGAC TGTCTGTCGC CTTTCGCCAC GCTCGCCGGA GCCACTCAAC CATACAAAGA 
TCACGTACTT GTGTCAAATT TTTATCCCGG GAGAACAGAC ACACACCTGA TATCAGCGCG 
GTTTCTTTTC TGCGGGCTCG AGAAGCGAGT TCTACCAGCG GTACAACTGA AACAGCAGCC 
AGTTTACAAA GTCATGCGAG AAAATACGAA GGACCACCGT ATGAAAGAGC AGTTGGTGAC 
ATTGTTCAGA GGTGCAGAGA AGAGCTCAAA TCTATTGGAG AAAACAACCC AGGCCTTAAA 
CCCACACTGG CTATCATACA GGCTGGTGAA GATGATGGTT TATTGGAAAT GAATAAGAAG 
ATGGCTGGAA AGATTGGCTT AAATGTGATG CAGATTTGTC TTCCACACCG GTGCACAGAA 
GAGGAGGTCA TTGAGGAGGT CTTGAGGTTA AATAAAGATT CTCGTGTTCA TGGCATTTTC 
CTGCACCTTC CTCAGACCTT CCTTTCCAAA AGTGTTCGTA ATGCCATCAA GCCACAGAAG 
GATGTGGATG GGGTGTGTGA TCTTAATGTT GGCCGTGTGG TGTTGGGTGA TAAGACAGAT 
GGCTTCACCT CACCTGTTGC TGGAGCTGTG CTGGAGCTGC TTGCCAAACA TGATGCTCCG 
TTAGTTGGCA AGATGGCAGT GCTGGTGGGT TTGGAGGGAC CCCTGAAGGT GGCTCTTCAA 
TGTCTTCTGC AAAACAATGG CATGATACTG CAAACCTGCA GGTGGAGCTC AACAAACCTG 
CAAAAACAGA TCATGGATTC AGATGTGGTG ATAACATTAA ACACAAACCA AAATCACATT 
CCATCAACAT GGTTAAGGCC AGGCGTGGCA GCCATCAACC TCGGTTCGGC ACTATTGGAA 
GAGCACCCTG ACAGCTGGGA GGCCACAGTT GAAGCCCAGT CTGCTGCAGG ACTTGGTCCT 
TTAACTGCTG CTTTTCGAAT GCAGCATGTG GTGAGGAGTA GCAGAAGGTG GATACAGGAA 
CAGCAGTACC AGCCATGGAA TATCCGGCCG CTAAAACTTC ACCCACTGTC ACCTGTGCCC 
AGTGATATCG AGATCTCCAG AGCACAAACT CCCAAACCCA TATCCCAGCT TGCCCAGGAG 
ATCGGCCTGC TACCAGAGGA GCTTGAGGCC TATGGGAACA CCAAAGCCAA AGTCCATTTA 
TCTCTCCTCA ACCGCCTCCA ACACCAGCCT GATGGCAGAT ATGTGCTGGT AGCAGGTATA 
ACTCCCACTC CTCTGGGCGA GGGGAAGAGT ACAGTGACCA TAGGTTTGGC TCAGGCTCTC 
ACCGCTCATC TCAAACTCAA CTCCTTCGCC TGCCTGAGAC AGCCTTCACA AGGCCCAACT 
TTTGGAGTCA AAGGAGGAGC AGCGGGTGGG GGATATGCAC AGGTCATTCC TATGGAGGAG 
TTTAATCTCC ACCTCACAGG AGACATCCAC GCTATAACCG CAGCTAATAA TCTAGTGGCG 
GCAGCTATTG ATGCCCGCAT TCTCCATGAA GCTACGCAAT CAGACAAGGC CCTGTACAGC 
AGACTAGTGC CATCTGTTAA TGGTGTGAGG CGCTTCTCTC CCATACAGCT GGCTCGTCTA 
CAGCGTCTTG GAATAAACAA ATCCAATCCC ACTGATCTCA CACCACAAGA GGTCAGTGCT 
TTTGTCCGTC TTGACCTTGA CCCTGAGAAG GTCACATGGC AAAGAGTTGT TGACACCAAT 
GACCGATTTC TGCGCAAGAT CACTATAGGC CAGGCCAACA CTGAAAAAGG CCATGCTCGC 
CAGACCCAGT TTGATATCGC GGTGGCCAGT GAGATCATGG CCATCCTCGC TCTGACTGAT 
GGATTGGCTG ACATGCGGGC CAGACTGGGG CGGATGGTTG TTGGCAGCAG CAGGAATGGA 
CAGGCGATCA CTGCAGATGA CCTGGGTGTG ACCGGGGCTT TGGCCGTCCT CATGAAAGAT 
GCTATCAAAC CCACACTAAT GCAGACACTG GAGGGCACTC CAGTGTTTGT GCATGCAGGG 
CCTTTCGCTA ACATCGCCCA TGGGAACTCC TCTGTCTTGG CAGATAAGCT GGCTCTTAAA 
CTAGTTGGAG AAGAGGGCTA TGTTGCGATG TGCTCCAGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Disruption of the folate pathway in zebrafish causes developmental defects.
BMC developmental biology1212(2012 Apr)
Lee MS,Bonner JR,Bernard DJ,Sanchez EL,Sause ET,Prentice RR,Burgess SM,Brody LC

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mthfd1l cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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