barhl2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	barhl2
Entrez Gene ID	100001699
Full Name	BarH-like homeobox 2
Synonyms	B-H2,wu:fq39g04,zgc:113948
General protein information	Preferred Names BarH-like homeobox 2 Names barH-like 2 homeobox protein BarH-like 2 barH-class homeodomain transcription factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205740.1	NP_991303.1	barH-like 2 homeobox protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	BARHL2	NP_001179226.1
Pan troglodytes (chimpanzee)	BARHL2	XP_003949506.1
Mus musculus (house mouse)	Barhl2	NP_001005477.1
Canis lupus familiaris (dog)	BARHL2	XP_853271.3
Rattus norvegicus (Norway rat)	Barhl2	NP_075245.1
Homo sapiens (human)	BARHL2	NP_064447.1
	BARHL2	XP_001089467.1
Danio rerio (zebrafish)	barhl2	NP_991303.1
Xenopus tropicalis (tropical clawed frog)	barhl2	XP_002940151.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression Profiling of Developing Zebrafish Retinal Cells.
Mullally M, Albrecht C, Horton M, Laboissonniere LA, Goetz JJ, Chowdhury R, Manning A, Wester AK, Bose Q, Trimarchi JM
Zebrafish13(4)272-80(2016 08)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Initiation of cyp26a1 Expression in the Zebrafish Anterior Neural Plate by a Novel Cis-Acting Element.
Chen C, Stedman A, Havis E, Anselme I, Onichtchouk D, Giudicelli F, Schneider-Maunoury S
PloS one11(3)e0150639(2016)

Irx7, a Smarca4-regulated gene for retinal differentiation, regulates other genes controlled by Smarca4 in zebrafish retinas.
Zhang Y, Bonilla S, Chong L, Leung YF
Gene expression patterns : GEP13(5-6)177-82(2013 Jun-Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Barhl2 is an amacrine-cell subtype identity-biasing factor that turns on within Atoh7-positive descendants.
Title: Biasing amacrine subtypes in the Atoh7 lineage through expression of Barhl2.

The following barhl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the barhl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33367	NM_205740.1 Latest version!	Danio rerio BarH-like homeobox 2 (barhl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

barhl2 ( NM_205740.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991303.1 Danio rerio BarH-like homeobox 2 (barhl2), mRNA.

Price & Availability↑

CloneID	ODa33367
Clone ID Related Accession (Same CDS sequence)	NM_205740.1
Accession Version	NM_205740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-12
Organism	Danio rerio(zebrafish)
Product	barH-like 2 homeobox protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY145141.1. On Feb 21, 2008 this sequence version replaced XM_001336612.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY145141.1, BC097030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGAT CCAGTGGGGC TAGTTTCGGA ATAGACACTA TTTTGTCCAC AACCAATTCT 
GGCACCTCAG TACTGATGAA CGGAGATTTT CGTCTCGGTG ACAGTAGCAG AACAGCGGAT 
TTCAGGAGCC AGGCGACCCC ATCACCATGT TCGGAGATAG ACACTGTGGG AACAGCCCCC 
TCATCCCCGA TTTCGGTCAC GATGGAGCAT CCCGAACCGC ATCTTGTCCA AGACAGCCTT 
CAGCATCACC ATCACCATCA TCATCACAGT CAGGCTCAGA GTTTGCAGCT TTCACCTCAG 
CCTCATCTGC TCGGTCAGGC TGGTTGTGCC CCCAGGACTG CCACCTCCTC CTTTCTAATC 
AAAGACATTT TGGGCGACAG TAAACCGCTT GCAGCGTGCG CTCCTTACAG CACCAGTGTA 
CCATCTCCAC ACCACAGCCC CAAAACAGAG AGCGGAACCG CCCCGGACGG CATCAGGCCC 
AAACTAGAAC AAGATGAAAA CAGAAGCAAA CTGGACAAAA GAGATGATAT CCAGAGTGAT 
TTGAAATGTT TGAACGGAAC AAAAGAAGAG GGCGATCGGG AAATTTCTAG TTCCAGAGAC 
AGTCCACCAG TCCGTTCTAA AAAACCTCGC AAAGCGCGGA CGGCCTTCTC CGACCATCAA 
CTTAACCAGC TGGAGCGCAG TTTTGAGAGA CAGAAATACC TCAGTGTACA GGACCGAATG 
GACCTGGCAG CAGCCCTAAA CCTCACAGAC ACACAAGTAA AGACTTGGTA TCAAAACCGA 
CGGACGAAAT GGAAGAGGCA GACGGCTGTC GGATTAGAAC TGCTGGCTGA AGCTGGAAAT 
TATTCGGCCT TACAGAGAAT GTTTCCTTCC CCGTATTTCT ATCATCCGAG TTTATTAGGA 
ACCGTGGACA GCACGACAGC GGCCGCAGCC GCCGCTGCCA TGTACAGCAG CATGTACCGG 
ACTCCGTCCA CACCGCATCC GTCTCTCCAG AGACCGCTCG TCCCCAGAGT GCTTATTCAC 
GGCCTGGGGC CCGGGGGACA ACCGGCACTA AACCCCATAC CAAACCCAAT TCCGGGCACA 
CCGCATGCTC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991303.1
CDS	400..1494
Translation	MEGSSGASFGIDTILSTTNSGTSVLMNGDFRLGDSSRTADFRSQATPSPCSEIDTVGTAPSSPISVTMEHPEPHLVQDSLQHHHHHHHHSQAQSLQLSPQPHLLGQAGCAPRTATSSFLIKDILGDSKPLAACAPYSTSVPSPHHSPKTESGTAPDGIRPKLEQDENRSKLDKRDDIQSDLKCLNGTKEEGDREISSSRDSPPVRSKKPRKARTAFSDHQLNQLERSFERQKYLSVQDRMDLAAALNLTDTQVKTWYQNRRTKWKRQTAVGLELLAEAGNYSALQRMFPSPYFYHPSLLGTVDSTTAAAAAAAMYSSMYRTPSTPHPSLQRPLVPRVLIHGLGPGGQPALNPIPNPIPGTPHAR

Target ORF information:

RefSeq Version	NM_205740.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BarH-like homeobox 2 (barhl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205740.1

ORF Insert Sequence:

ATGGAAGGAT CCAGTGGGGC TAGTTTCGGA ATAGACACTA TTTTGTCCAC AACCAATTCT 
GGCACCTCAG TACTGATGAA CGGAGATTTT CGTCTCGGTG ACAGTAGCAG AACAGCGGAT 
TTCAGGAGCC AGGCGACCCC ATCACCATGT TCGGAGATAG ACACTGTGGG AACAGCCCCC 
TCATCCCCGA TTTCGGTCAC GATGGAGCAT CCCGAACCGC ATCTTGTCCA AGACAGCCTT 
CAGCATCACC ATCACCATCA TCATCACAGT CAGGCTCAGA GTTTGCAGCT TTCACCTCAG 
CCTCATCTGC TCGGTCAGGC TGGTTGTGCC CCCAGGACTG CCACCTCCTC CTTTCTAATC 
AAAGACATTT TGGGCGACAG TAAACCGCTT GCAGCGTGCG CTCCTTACAG CACCAGTGTA 
CCATCTCCAC ACCACAGCCC CAAAACAGAG AGCGGAACCG CCCCGGACGG CATCAGGCCC 
AAACTAGAAC AAGATGAAAA CAGAAGCAAA CTGGACAAAA GAGATGATAT CCAGAGTGAT 
TTGAAATGTT TGAACGGAAC AAAAGAAGAG GGCGATCGGG AAATTTCTAG TTCCAGAGAC 
AGTCCACCAG TCCGTTCTAA AAAACCTCGC AAAGCGCGGA CGGCCTTCTC CGACCATCAA 
CTTAACCAGC TGGAGCGCAG TTTTGAGAGA CAGAAATACC TCAGTGTACA GGACCGAATG 
GACCTGGCAG CAGCCCTAAA CCTCACAGAC ACACAAGTAA AGACTTGGTA TCAAAACCGA 
CGGACGAAAT GGAAGAGGCA GACGGCTGTC GGATTAGAAC TGCTGGCTGA AGCTGGAAAT 
TATTCGGCCT TACAGAGAAT GTTTCCTTCC CCGTATTTCT ATCATCCGAG TTTATTAGGA 
ACCGTGGACA GCACGACAGC GGCCGCAGCC GCCGCTGCCA TGTACAGCAG CATGTACCGG 
ACTCCGTCCA CACCGCATCC GTCTCTCCAG AGACCGCTCG TCCCCAGAGT GCTTATTCAC 
GGCCTGGGGC CCGGGGGACA ACCGGCACTA AACCCCATAC CAAACCCAAT TCCGGGCACA 
CCGCATGCTC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression Profiling of Developing Zebrafish Retinal Cells.
Zebrafish13(4)272-80(2016 08)
Mullally M,Albrecht C,Horton M,Laboissonniere LA,Goetz JJ,Chowdhury R,Manning A,Wester AK,Bose Q,Trimarchi JM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Initiation of cyp26a1 Expression in the Zebrafish Anterior Neural Plate by a Novel Cis-Acting Element.
PloS one11(3)e0150639(2016)
Chen C,Stedman A,Havis E,Anselme I,Onichtchouk D,Giudicelli F,Schneider-Maunoury S

Irx7, a Smarca4-regulated gene for retinal differentiation, regulates other genes controlled by Smarca4 in zebrafish retinas.
Gene expression patterns : GEP13(5-6)177-82(2013 Jun-Jul)
Zhang Y,Bonilla S,Chong L,Leung YF