bbs10 cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene
Clones
gRNAs

Summary

Gene Symbol	bbs10
Entrez Gene ID	107102391
Full Name	Bardet-Biedl syndrome 10
Gene Type	protein-coding
Organism	Cyprinodon variegatus(sheepshead minnow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015401679.1	XP_015257165.1	Bardet-Biedl syndrome 10 protein

Pathways from BioSystems
Gene Ontology

The following bbs10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bbs10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy29166	XM_015401679.1 Latest version!	Cyprinodon variegatus Bardet-Biedl syndrome 10 (bbs10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bbs10 ( XM_015401679.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015257165.1 Cyprinodon variegatus Bardet-Biedl syndrome 10 (bbs10), mRNA.

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CloneID	OCy29166
Clone ID Related Accession (Same CDS sequence)	XM_015401679.1
Accession Version	XM_015401679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	Bardet-Biedl syndrome 10 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015151702.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGAGT CGGGGGACCT CCACCTGAAA CATGTCCTAC AGTCAGTGTG TGCCCTAGAG 
TCCGTCATCC TTCGCAGTTT TGGTCCTGAA GGAGGACAGG TGCTGTTCAC CCGTGACACA 
GGACAGTTGC TGTTGAGCCG CAGCGGAAAA CGCATCCTCA CTGCTCTACA TCTGGAGCAT 
CCACTGGCCA GGATGGTGGT GGATTGCGTC TGGAAACACA GCACAGCAAC CGGTGATGGA 
TCTAAAACCT TTGTTCTAAT CCTTGCATCC CTGCTGCGTT TGATTCAGGC TGCAGCCACT 
AAGGGAGCCC ATACATCTCA TTCTTATACC TCCACAGAAA TGGCAGAAGC TGCCACTGCC 
AGACACTTGG CTGACAGACT GCTGGAATTC GTGTTGACTG AGTTGGAAGC ACTCATTGCA 
GTCAATGTGT TGCCACATGG ATTCCATATT TCATTAGAGG AAGTTACAGA GAAATCGCAA 
CATAATAGGA GTTTTCAAAC CCTTCTTTTA TCATTTTTTC ATACACGCCT CGGTAGTGTG 
TATTGTGACT TTTTCAGGGA CCTCACGTGT GAAATGCTCA GTCACTGGAG GGTAAAAGAT 
CCCCGTCATT CATTCTCCAT TCAGTTTTTA AACAACAATT TCCCAGCGCT ACATACCCAA 
GTATCAGGCT TCCCAGTTGG CTCCTCCCAT TTGATAGAGG GGCAGGTGAT TCACAGAGAC 
TTTGCTACAC CTTGCCCTCC AGTGAAAAAG CAACTTTTCA AAGCTGTGGT TTTCACTGGA 
TACCTGCAGC CAAAAGTCCT CGCTGCAGGT GAAGTGCTTG AACTGGGCTG TGGAGACATG 
GTGACAAAGG ACAGCAGCAT TGTGCACTTT AGTTCCTGGA CAGAGGGGTC ACTGAAGAGC 
ATAATTGCTT CTCTGCAGAG TTTTGGCGTC TCTGTCCTTT TGTGTGCGCT GAAACAGTCC 
CCTGCTGCCC TGGCTTTAGC AACACAGTCA GGGATGTGCA TTGTGGAGTG TGTCAGTGAA 
GAGGAACTGT CTCTTTTTGC CCAGCTCAGT GGGGTAACAC CTGTCTCAGA CTGCTTGGGG 
ATTCAACCAG AGCACATTGC AACCCTGACT TTCTGTAAAC CTTTACAGCT TGGAGCCCAA 
AGATATGTTC ATTTGGACTT CCAGGACTCT GAAGAAAGGC TCAACGTCAA ACCTTGCAGC 
CTGGTGATTT GTGCTCCAGG GGAGGGGCAA ACGGATCAGT ATGCATGTGC ATTTCAAGAT 
GTGTTTCGAA TGCTACTGAC AGCATGGCCG CCCAGACGCG AGACTAACAC CACAGGTTGT 
CAGAGAACCT TTCCATCCAA TAGTTGCACA TCTTTACATT CAGACGCTAT AAGTGCTGGA 
ACTGCATACA CATTGTGCAG TGTGCATCCC AGTCAGAAGT GTGTGATAGA GCCAGGTTTT 
GTAATATCTG CAGGTGGGAC GTTTGAGTTT CTGCTGAACC ATGCCCTTCA TCAATATGAC 
CGCAGTTGCT CGGTTTCTGT TAACACAAGT GTAGTTGTTC CAGTTGCCCA GCTCTTGGCA 
GAAGCACTGT TGAGTGTGCC TCGGCTGATT TACTCTCACA ATCTAAAACG TTTCCTGCAG 
GAACAAACCA GGGTCATGGC TTTAATTCCA AAACAATCCC ACACCCTCAG TGCAAGCCTC 
AGACAGACTG AAGGTCCTCC TGAAGATGGT AAACTAAGCC TACACTCTTG TGAAAAGGGT 
AACATGGCAT TGAAACTTTT GGACTCAGGC CTTGAANNNN NNNNNNNNNN NTATCATCTG 
GTTCTGGCTG TGCTGCAGTG TCTCACAAGT CTCCTCCGAG TGAACAAGGA GCTACGGATA 
CACACCACTT TACACAGAAA ATCCTTTAAA CTCCTGAGCA CCTGTTCAGA GAGTACAGAA 
GACGAAGAAG AGGCGTTGCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015257165.1
CDS	37..1980
Translation	MPESGDLHLKHVLQSVCALESVILRSFGPEGGQVLFTRDTGQLLLSRSGKRILTALHLEHPLARMVVDCVWKHSTATGDGSKTFVLILASLLRLIQAAATKGAHTSHSYTSTEMAEAATARHLADRLLEFVLTELEALIAVNVLPHGFHISLEEVTEKSQHNRSFQTLLLSFFHTRLGSVYCDFFRDLTCEMLSHWRVKDPRHSFSIQFLNNNFPALHTQVSGFPVGSSHLIEGQVIHRDFATPCPPVKKQLFKAVVFTGYLQPKVLAAGEVLELGCGDMVTKDSSIVHFSSWTEGSLKSIIASLQSFGVSVLLCALKQSPAALALATQSGMCIVECVSEEELSLFAQLSGVTPVSDCLGIQPEHIATLTFCKPLQLGAQRYVHLDFQDSEERLNVKPCSLVICAPGEGQTDQYACAFQDVFRMLLTAWPPRRETNTTGCQRTFPSNSCTSLHSDAISAGTAYTLCSVHPSQKCVIEPGFVISAGGTFEFLLNHALHQYDRSCSVSVNTSVVVPVAQLLAEALLSVPRLIYSHNLKRFLQEQTRVMALIPKQSHTLSASLRQTEGPPEDGKLSLHSCEKGNMALKLLDSGLEnullnullnullnullnullYHLVLAVLQCLTSLLRVNKELRIHTTLHRKSFKLLSTCSESTEDEEEALL

Target ORF information:

RefSeq Version	XM_015401679.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus Bardet-Biedl syndrome 10 (bbs10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015401679.1

ORF Insert Sequence:

ATGCCGGAGT CGGGGGACCT CCACCTGAAA CATGTCCTAC AGTCAGTGTG TGCCCTAGAG 
TCCGTCATCC TTCGCAGTTT TGGTCCTGAA GGAGGACAGG TGCTGTTCAC CCGTGACACA 
GGACAGTTGC TGTTGAGCCG CAGCGGAAAA CGCATCCTCA CTGCTCTACA TCTGGAGCAT 
CCACTGGCCA GGATGGTGGT GGATTGCGTC TGGAAACACA GCACAGCAAC CGGTGATGGA 
TCTAAAACCT TTGTTCTAAT CCTTGCATCC CTGCTGCGTT TGATTCAGGC TGCAGCCACT 
AAGGGAGCCC ATACATCTCA TTCTTATACC TCCACAGAAA TGGCAGAAGC TGCCACTGCC 
AGACACTTGG CTGACAGACT GCTGGAATTC GTGTTGACTG AGTTGGAAGC ACTCATTGCA 
GTCAATGTGT TGCCACATGG ATTCCATATT TCATTAGAGG AAGTTACAGA GAAATCGCAA 
CATAATAGGA GTTTTCAAAC CCTTCTTTTA TCATTTTTTC ATACACGCCT CGGTAGTGTG 
TATTGTGACT TTTTCAGGGA CCTCACGTGT GAAATGCTCA GTCACTGGAG GGTAAAAGAT 
CCCCGTCATT CATTCTCCAT TCAGTTTTTA AACAACAATT TCCCAGCGCT ACATACCCAA 
GTATCAGGCT TCCCAGTTGG CTCCTCCCAT TTGATAGAGG GGCAGGTGAT TCACAGAGAC 
TTTGCTACAC CTTGCCCTCC AGTGAAAAAG CAACTTTTCA AAGCTGTGGT TTTCACTGGA 
TACCTGCAGC CAAAAGTCCT CGCTGCAGGT GAAGTGCTTG AACTGGGCTG TGGAGACATG 
GTGACAAAGG ACAGCAGCAT TGTGCACTTT AGTTCCTGGA CAGAGGGGTC ACTGAAGAGC 
ATAATTGCTT CTCTGCAGAG TTTTGGCGTC TCTGTCCTTT TGTGTGCGCT GAAACAGTCC 
CCTGCTGCCC TGGCTTTAGC AACACAGTCA GGGATGTGCA TTGTGGAGTG TGTCAGTGAA 
GAGGAACTGT CTCTTTTTGC CCAGCTCAGT GGGGTAACAC CTGTCTCAGA CTGCTTGGGG 
ATTCAACCAG AGCACATTGC AACCCTGACT TTCTGTAAAC CTTTACAGCT TGGAGCCCAA 
AGATATGTTC ATTTGGACTT CCAGGACTCT GAAGAAAGGC TCAACGTCAA ACCTTGCAGC 
CTGGTGATTT GTGCTCCAGG GGAGGGGCAA ACGGATCAGT ATGCATGTGC ATTTCAAGAT 
GTGTTTCGAA TGCTACTGAC AGCATGGCCG CCCAGACGCG AGACTAACAC CACAGGTTGT 
CAGAGAACCT TTCCATCCAA TAGTTGCACA TCTTTACATT CAGACGCTAT AAGTGCTGGA 
ACTGCATACA CATTGTGCAG TGTGCATCCC AGTCAGAAGT GTGTGATAGA GCCAGGTTTT 
GTAATATCTG CAGGTGGGAC GTTTGAGTTT CTGCTGAACC ATGCCCTTCA TCAATATGAC 
CGCAGTTGCT CGGTTTCTGT TAACACAAGT GTAGTTGTTC CAGTTGCCCA GCTCTTGGCA 
GAAGCACTGT TGAGTGTGCC TCGGCTGATT TACTCTCACA ATCTAAAACG TTTCCTGCAG 
GAACAAACCA GGGTCATGGC TTTAATTCCA AAACAATCCC ACACCCTCAG TGCAAGCCTC 
AGACAGACTG AAGGTCCTCC TGAAGATGGT AAACTAAGCC TACACTCTTG TGAAAAGGGT 
AACATGGCAT TGAAACTTTT GGACTCAGGC CTTGAANNNN NNNNNNNNNN NTATCATCTG 
GTTCTGGCTG TGCTGCAGTG TCTCACAAGT CTCCTCCGAG TGAACAAGGA GCTACGGATA 
CACACCACTT TACACAGAAA ATCCTTTAAA CTCCTGAGCA CCTGTTCAGA GAGTACAGAA 
GACGAAGAAG AGGCGTTGCT CTAG