rasef cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	rasef
Entrez Gene ID	103398919
Full Name	RAS and EF-hand domain containing
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008337673.3	XP_008335895.1	ras and EF-hand domain-containing protein
XM_008337673.2	XP_008335895.1	ras and EF-hand domain-containing protein

Pathways from BioSystems
Gene Ontology

The following rasef gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rasef cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy33042	XM_008337673.3 Latest version!	Cynoglossus semilaevis RAS and EF-hand domain containing (rasef), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OCy33042	XM_008337673.2	Cynoglossus semilaevis RAS and EF-hand domain containing (rasef), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rasef ( XM_008337673.3 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008335895.1 Cynoglossus semilaevis RAS and EF-hand domain containing (rasef), mRNA.

Price & Availability↑

CloneID	OCy33042
Clone ID Related Accession (Same CDS sequence)	XM_008337673.3 , XM_008337673.2
Accession Version	XM_008337673.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	ras and EF-hand domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007585157.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_008337673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 15% of CDS bases ##RefSeq-Attributes-END##

ATGGGAATAG AGGAGTTGCA GGTGGGCAGG TTGAAGGTGG TGTTGGTACC GGCAGAGTGG 
AAGGAGGGAG GTGTGATGTT GTGGAGCTTG GGTGGTAGGC CACTGAGAGA GGGAGTGGAG 
GAGGCTGCAA TGAGGATGTA TCCAGGCCTG AAGCATCTCA ACATTGGACA AGTGCCTAAA 
GTCAAGGTGC CCAGCCTCAC AGTCATGATG TCACACCTTA AATTTCTCCT GACTCTAAAT 
CTCACTGGTC TTCAGGCAAT CTCTGGATTG ACCATTGAGG ATTTGACCAG CAGCTCAGAA 
GAACAGCACA AAGATAAGGA AGATAAGTGC AAGGCGGAGG ACACCTTCAC TGAGAAGATT 
ACCAACAAGC AGCTGAAGTT GTCAAGTCAG CCTTATGTCT CCATCAGTGG CGTGAGCTCA 
GAGGAGCGTG ATCGGCTACG CTCTCTCTTT CACGCCTACG ACGTGAACAA TTCTGGGAGG 
ATCGAAAGAC GGCAGTTCTT CACCATCTGT GCGCTGCTCC AAGTGTCCAC AGCCGCGGCG 
CAGAGCGTGT TTGACAGGCT TGACGTGGGC GAGGACGGCA CGGTCACCGT GCTGGAGTTT 
ATCAGCGGCT TCTACGATCG CTACGGTGAA GACATGGAGA GTGACGGAGC AGAGGTCTGG 
TCTGCTGCCT GGGAAGACTT CTCCGGCAGA CTCGGGGCAC AGGCCAAGTT CATCCCCAGA 
AATGAGCAAG CAGCAATGTT GTACCAGAAC ATCAGCCTGA CAGAACCCAG ACTGATACCC 
CAGTTTGAAA AAGTCATCCT GAACTTCACC AAAGAGATCA GGCAGCAAAA CCTGGAGATG 
GAGAACCTGG CTCTGGCTAT CAAAAGGGCA CAGGACCAGG CATCTATGCA ACTGAGTGAG 
ATGGAGGAGG AGATGGACCA GCGCATCCAG GCTGCAGAGA AAACAACACG AGAACAGGAG 
AAGATGCGAG CAGAGGCAGA ACTGAGTGAG ATACGGAGAG ACTTTGAAAC TGAAGTGTGT 
GAACTTCAGT GTAAGATCCA GAAGATGCAG TTGATTGAAG AGAAGTACAT GAACATCACA 
GTGAAAGACC AGTCTCTGAC ATTAAAGAAG ACGATCTCTG AGATGACGCT GGAAAACCAG 
CGACTGAAGC AGGAGCTGCT GAAATCTCAG ACCAAAGTCG CCTGTTTACA CAGTGAGATG 
GACTTACTGA AGACCGAGTT AGCTGATCAA AGTATCAACT CTGAAAGAGA TGAGGAGCTC 
ATGCACCAGT TCTCTGAAGA ACGTGACATT CTGGAGAGTC AGATTGAGAT TTTGCAACAA 
GCTAACAGAA AGCTTCATGA CACTAATGAT GGTCTGAGGA CAACTCTGGA AAGGATTTCA 
AGGTCTGGTA ACAGTGAGTC CCCAGTAGAA ATGAAGAGCA GGAACAGGAG TAAGAGCATC 
TGTTACACAT CGCCGTATGC GTTAATAGAC AGGTACTGCC AGCGTATGAA CGACAACTTC 
TGTCCGTACA ACCATCAACC CAGCTCCGAC ACACTGGCCT TGGCCATGTG TGACCCCGGC 
CTGATGCGCA GGAACAGCAG TGAGTGTGAA GAGGACAGTC TGCCTGAATT CTACATGGAC 
AGCGGCCTGT CCACACTCAG GGGCTCCCAT GGTGGTTACG ACTCCGAGCA GGAGGTTAAA 
GGTCACGAAG ATGAAGACGA AGAGGAGGAG GAAGAGAGGG GGAAGAAGAC AGTGGAGGAT 
GACAGAATGA TGGGAGAAAA CTCAGATACT GAGCCAGCAG AGACACAGGA CACCGAATCT 
GCATTTGGTT CGGACAGCAG CTCAGTGTTG GACTGGAAAC TGCCTGAGAT CACTAGTGAC 
AGCAAAACAT CGAAACCCCA AACGAGGGCT CTCAGTGCCA TCAGTGTTCA GAAGGATGAA 
GATAATGTTA ACCGGGGCTA CATGACGTCA GAGAAGGCCT TCAGAATCGT TTTGGCAGGA 
GACGCTGCTG TGGGAAAGTC CAGCTTCCTG CTGCGCCTTT GTAAGAATGA ATTTATGCCA 
AGCTCCAGTG CAACCTTGGG AGTGGATTTC CAGATCAAGA CACTGGTAGT TGATGGAGAT 
CCTGTTCTAC TTCAGTTATG GGACACTGCA GGACAGGAAA GGTTCCGCAG CATTGCTAAG 
TCATATTTCC GTCGAGCGGA CGGTGTTTTG CTGCTGTATG ACGTCACCTG TGAGAGAAGT 
TTCCTTAACG TGAGAGAGTG GGTGGATATG ATAGAGGACC TATCTCCAGA CAATATTTCC 
ATCATGCTCG TGGGCAATAA GTGTGACCTG AGACAAGACG GAGCTAACTT TGTTCCTACC 
AGCTATGGTG AAAAGCTGGC CATGACATAC AACACTCTGT TCTGTGAGAC TAGTGCCAAA 
GATGGTTCCA ACATCCTGGA GGCTGTGTTG CATTTGGCCA GGCAAGTCAA ACAGTACGCT 
TCTTTAAATA AAAAGGCTGA CCATCAGTCC CTGCCCAGTC TGGATGCTCC CAGGAAGAAA 
CCAAACCTCT CCTGCTGCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008335895.1
CDS	1..2544
Translation	MGIEELQVGRLKVVLVPAEWKEGGVMLWSLGGRPLREGVEEAAMRMYPGLKHLNIGQVPKVKVPSLTVMMSHLKFLLTLNLTGLQAISGLTIEDLTSSSEEQHKDKEDKCKAEDTFTEKITNKQLKLSSQPYVSISGVSSEERDRLRSLFHAYDVNNSGRIERRQFFTICALLQVSTAAAQSVFDRLDVGEDGTVTVLEFISGFYDRYGEDMESDGAEVWSAAWEDFSGRLGAQAKFIPRNEQAAMLYQNISLTEPRLIPQFEKVILNFTKEIRQQNLEMENLALAIKRAQDQASMQLSEMEEEMDQRIQAAEKTTREQEKMRAEAELSEIRRDFETEVCELQCKIQKMQLIEEKYMNITVKDQSLTLKKTISEMTLENQRLKQELLKSQTKVACLHSEMDLLKTELADQSINSERDEELMHQFSEERDILESQIEILQQANRKLHDTNDGLRTTLERISRSGNSESPVEMKSRNRSKSICYTSPYALIDRYCQRMNDNFCPYNHQPSSDTLALAMCDPGLMRRNSSECEEDSLPEFYMDSGLSTLRGSHGGYDSEQEVKGHEDEDEEEEEERGKKTVEDDRMMGENSDTEPAETQDTESAFGSDSSSVLDWKLPEITSDSKTSKPQTRALSAISVQKDEDNVNRGYMTSEKAFRIVLAGDAAVGKSSFLLRLCKNEFMPSSSATLGVDFQIKTLVVDGDPVLLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCERSFLNVREWVDMIEDLSPDNISIMLVGNKCDLRQDGANFVPTSYGEKLAMTYNTLFCETSAKDGSNILEAVLHLARQVKQYASLNKKADHQSLPSLDAPRKKPNLSCCT

Target ORF information:

RefSeq Version	XM_008337673.3
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis RAS and EF-hand domain containing (rasef), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008337673.3

ORF Insert Sequence:

ATGGGAATAG AGGAGTTGCA GGTGGGCAGG TTGAAGGTGG TGTTGGTACC GGCAGAGTGG 
AAGGAGGGAG GTGTGATGTT GTGGAGCTTG GGTGGTAGGC CACTGAGAGA GGGAGTGGAG 
GAGGCTGCAA TGAGGATGTA TCCAGGCCTG AAGCATCTCA ACATTGGACA AGTGCCTAAA 
GTCAAGGTGC CCAGCCTCAC AGTCATGATG TCACACCTTA AATTTCTCCT GACTCTAAAT 
CTCACTGGTC TTCAGGCAAT CTCTGGATTG ACCATTGAGG ATTTGACCAG CAGCTCAGAA 
GAACAGCACA AAGATAAGGA AGATAAGTGC AAGGCGGAGG ACACCTTCAC TGAGAAGATT 
ACCAACAAGC AGCTGAAGTT GTCAAGTCAG CCTTATGTCT CCATCAGTGG CGTGAGCTCA 
GAGGAGCGTG ATCGGCTACG CTCTCTCTTT CACGCCTACG ACGTGAACAA TTCTGGGAGG 
ATCGAAAGAC GGCAGTTCTT CACCATCTGT GCGCTGCTCC AAGTGTCCAC AGCCGCGGCG 
CAGAGCGTGT TTGACAGGCT TGACGTGGGC GAGGACGGCA CGGTCACCGT GCTGGAGTTT 
ATCAGCGGCT TCTACGATCG CTACGGTGAA GACATGGAGA GTGACGGAGC AGAGGTCTGG 
TCTGCTGCCT GGGAAGACTT CTCCGGCAGA CTCGGGGCAC AGGCCAAGTT CATCCCCAGA 
AATGAGCAAG CAGCAATGTT GTACCAGAAC ATCAGCCTGA CAGAACCCAG ACTGATACCC 
CAGTTTGAAA AAGTCATCCT GAACTTCACC AAAGAGATCA GGCAGCAAAA CCTGGAGATG 
GAGAACCTGG CTCTGGCTAT CAAAAGGGCA CAGGACCAGG CATCTATGCA ACTGAGTGAG 
ATGGAGGAGG AGATGGACCA GCGCATCCAG GCTGCAGAGA AAACAACACG AGAACAGGAG 
AAGATGCGAG CAGAGGCAGA ACTGAGTGAG ATACGGAGAG ACTTTGAAAC TGAAGTGTGT 
GAACTTCAGT GTAAGATCCA GAAGATGCAG TTGATTGAAG AGAAGTACAT GAACATCACA 
GTGAAAGACC AGTCTCTGAC ATTAAAGAAG ACGATCTCTG AGATGACGCT GGAAAACCAG 
CGACTGAAGC AGGAGCTGCT GAAATCTCAG ACCAAAGTCG CCTGTTTACA CAGTGAGATG 
GACTTACTGA AGACCGAGTT AGCTGATCAA AGTATCAACT CTGAAAGAGA TGAGGAGCTC 
ATGCACCAGT TCTCTGAAGA ACGTGACATT CTGGAGAGTC AGATTGAGAT TTTGCAACAA 
GCTAACAGAA AGCTTCATGA CACTAATGAT GGTCTGAGGA CAACTCTGGA AAGGATTTCA 
AGGTCTGGTA ACAGTGAGTC CCCAGTAGAA ATGAAGAGCA GGAACAGGAG TAAGAGCATC 
TGTTACACAT CGCCGTATGC GTTAATAGAC AGGTACTGCC AGCGTATGAA CGACAACTTC 
TGTCCGTACA ACCATCAACC CAGCTCCGAC ACACTGGCCT TGGCCATGTG TGACCCCGGC 
CTGATGCGCA GGAACAGCAG TGAGTGTGAA GAGGACAGTC TGCCTGAATT CTACATGGAC 
AGCGGCCTGT CCACACTCAG GGGCTCCCAT GGTGGTTACG ACTCCGAGCA GGAGGTTAAA 
GGTCACGAAG ATGAAGACGA AGAGGAGGAG GAAGAGAGGG GGAAGAAGAC AGTGGAGGAT 
GACAGAATGA TGGGAGAAAA CTCAGATACT GAGCCAGCAG AGACACAGGA CACCGAATCT 
GCATTTGGTT CGGACAGCAG CTCAGTGTTG GACTGGAAAC TGCCTGAGAT CACTAGTGAC 
AGCAAAACAT CGAAACCCCA AACGAGGGCT CTCAGTGCCA TCAGTGTTCA GAAGGATGAA 
GATAATGTTA ACCGGGGCTA CATGACGTCA GAGAAGGCCT TCAGAATCGT TTTGGCAGGA 
GACGCTGCTG TGGGAAAGTC CAGCTTCCTG CTGCGCCTTT GTAAGAATGA ATTTATGCCA 
AGCTCCAGTG CAACCTTGGG AGTGGATTTC CAGATCAAGA CACTGGTAGT TGATGGAGAT 
CCTGTTCTAC TTCAGTTATG GGACACTGCA GGACAGGAAA GGTTCCGCAG CATTGCTAAG 
TCATATTTCC GTCGAGCGGA CGGTGTTTTG CTGCTGTATG ACGTCACCTG TGAGAGAAGT 
TTCCTTAACG TGAGAGAGTG GGTGGATATG ATAGAGGACC TATCTCCAGA CAATATTTCC 
ATCATGCTCG TGGGCAATAA GTGTGACCTG AGACAAGACG GAGCTAACTT TGTTCCTACC 
AGCTATGGTG AAAAGCTGGC CATGACATAC AACACTCTGT TCTGTGAGAC TAGTGCCAAA 
GATGGTTCCA ACATCCTGGA GGCTGTGTTG CATTTGGCCA GGCAAGTCAA ACAGTACGCT 
TCTTTAAATA AAAAGGCTGA CCATCAGTCC CTGCCCAGTC TGGATGCTCC CAGGAAGAAA 
CCAAACCTCT CCTGCTGCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rasef ( XM_008337673.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008335895.1 Cynoglossus semilaevis RAS and EF-hand domain containing (rasef), mRNA.

Price & Availability↑

CloneID	OCy33042
Clone ID Related Accession (Same CDS sequence)	XM_008337673.3 , XM_008337673.2
Accession Version	XM_008337673.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	ras and EF-hand domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007585157.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008337673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 15% of CDS bases ##RefSeq-Attributes-END##

ATGGGAATAG AGGAGTTGCA GGTGGGCAGG TTGAAGGTGG TGTTGGTACC GGCAGAGTGG 
AAGGAGGGAG GTGTGATGTT GTGGAGCTTG GGTGGTAGGC CACTGAGAGA GGGAGTGGAG 
GAGGCTGCAA TGAGGATGTA TCCAGGCCTG AAGCATCTCA ACATTGGACA AGTGCCTAAA 
GTCAAGGTGC CCAGCCTCAC AGTCATGATG TCACACCTTA AATTTCTCCT GACTCTAAAT 
CTCACTGGTC TTCAGGCAAT CTCTGGATTG ACCATTGAGG ATTTGACCAG CAGCTCAGAA 
GAACAGCACA AAGATAAGGA AGATAAGTGC AAGGCGGAGG ACACCTTCAC TGAGAAGATT 
ACCAACAAGC AGCTGAAGTT GTCAAGTCAG CCTTATGTCT CCATCAGTGG CGTGAGCTCA 
GAGGAGCGTG ATCGGCTACG CTCTCTCTTT CACGCCTACG ACGTGAACAA TTCTGGGAGG 
ATCGAAAGAC GGCAGTTCTT CACCATCTGT GCGCTGCTCC AAGTGTCCAC AGCCGCGGCG 
CAGAGCGTGT TTGACAGGCT TGACGTGGGC GAGGACGGCA CGGTCACCGT GCTGGAGTTT 
ATCAGCGGCT TCTACGATCG CTACGGTGAA GACATGGAGA GTGACGGAGC AGAGGTCTGG 
TCTGCTGCCT GGGAAGACTT CTCCGGCAGA CTCGGGGCAC AGGCCAAGTT CATCCCCAGA 
AATGAGCAAG CAGCAATGTT GTACCAGAAC ATCAGCCTGA CAGAACCCAG ACTGATACCC 
CAGTTTGAAA AAGTCATCCT GAACTTCACC AAAGAGATCA GGCAGCAAAA CCTGGAGATG 
GAGAACCTGG CTCTGGCTAT CAAAAGGGCA CAGGACCAGG CATCTATGCA ACTGAGTGAG 
ATGGAGGAGG AGATGGACCA GCGCATCCAG GCTGCAGAGA AAACAACACG AGAACAGGAG 
AAGATGCGAG CAGAGGCAGA ACTGAGTGAG ATACGGAGAG ACTTTGAAAC TGAAGTGTGT 
GAACTTCAGT GTAAGATCCA GAAGATGCAG TTGATTGAAG AGAAGTACAT GAACATCACA 
GTGAAAGACC AGTCTCTGAC ATTAAAGAAG ACGATCTCTG AGATGACGCT GGAAAACCAG 
CGACTGAAGC AGGAGCTGCT GAAATCTCAG ACCAAAGTCG CCTGTTTACA CAGTGAGATG 
GACTTACTGA AGACCGAGTT AGCTGATCAA AGTATCAACT CTGAAAGAGA TGAGGAGCTC 
ATGCACCAGT TCTCTGAAGA ACGTGACATT CTGGAGAGTC AGATTGAGAT TTTGCAACAA 
GCTAACAGAA AGCTTCATGA CACTAATGAT GGTCTGAGGA CAACTCTGGA AAGGATTTCA 
AGGTCTGGTA ACAGTGAGTC CCCAGTAGAA ATGAAGAGCA GGAACAGGAG TAAGAGCATC 
TGTTACACAT CGCCGTATGC GTTAATAGAC AGGTACTGCC AGCGTATGAA CGACAACTTC 
TGTCCGTACA ACCATCAACC CAGCTCCGAC ACACTGGCCT TGGCCATGTG TGACCCCGGC 
CTGATGCGCA GGAACAGCAG TGAGTGTGAA GAGGACAGTC TGCCTGAATT CTACATGGAC 
AGCGGCCTGT CCACACTCAG GGGCTCCCAT GGTGGTTACG ACTCCGAGCA GGAGGTTAAA 
GGTCACGAAG ATGAAGACGA AGAGGAGGAG GAAGAGAGGG GGAAGAAGAC AGTGGAGGAT 
GACAGAATGA TGGGAGAAAA CTCAGATACT GAGCCAGCAG AGACACAGGA CACCGAATCT 
GCATTTGGTT CGGACAGCAG CTCAGTGTTG GACTGGAAAC TGCCTGAGAT CACTAGTGAC 
AGCAAAACAT CGAAACCCCA AACGAGGGCT CTCAGTGCCA TCAGTGTTCA GAAGGATGAA 
GATAATGTTA ACCGGGGCTA CATGACGTCA GAGAAGGCCT TCAGAATCGT TTTGGCAGGA 
GACGCTGCTG TGGGAAAGTC CAGCTTCCTG CTGCGCCTTT GTAAGAATGA ATTTATGCCA 
AGCTCCAGTG CAACCTTGGG AGTGGATTTC CAGATCAAGA CACTGGTAGT TGATGGAGAT 
CCTGTTCTAC TTCAGTTATG GGACACTGCA GGACAGGAAA GGTTCCGCAG CATTGCTAAG 
TCATATTTCC GTCGAGCGGA CGGTGTTTTG CTGCTGTATG ACGTCACCTG TGAGAGAAGT 
TTCCTTAACG TGAGAGAGTG GGTGGATATG ATAGAGGACC TATCTCCAGA CAATATTTCC 
ATCATGCTCG TGGGCAATAA GTGTGACCTG AGACAAGACG GAGCTAACTT TGTTCCTACC 
AGCTATGGTG AAAAGCTGGC CATGACATAC AACACTCTGT TCTGTGAGAC TAGTGCCAAA 
GATGGTTCCA ACATCCTGGA GGCTGTGTTG CATTTGGCCA GGCAAGTCAA ACAGTACGCT 
TCTTTAAATA AAAAGGCTGA CCATCAGTCC CTGCCCAGTC TGGATGCTCC CAGGAAGAAA 
CCAAACCTCT CCTGCTGCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008335895.1
CDS	1..2544
Translation	MGIEELQVGRLKVVLVPAEWKEGGVMLWSLGGRPLREGVEEAAMRMYPGLKHLNIGQVPKVKVPSLTVMMSHLKFLLTLNLTGLQAISGLTIEDLTSSSEEQHKDKEDKCKAEDTFTEKITNKQLKLSSQPYVSISGVSSEERDRLRSLFHAYDVNNSGRIERRQFFTICALLQVSTAAAQSVFDRLDVGEDGTVTVLEFISGFYDRYGEDMESDGAEVWSAAWEDFSGRLGAQAKFIPRNEQAAMLYQNISLTEPRLIPQFEKVILNFTKEIRQQNLEMENLALAIKRAQDQASMQLSEMEEEMDQRIQAAEKTTREQEKMRAEAELSEIRRDFETEVCELQCKIQKMQLIEEKYMNITVKDQSLTLKKTISEMTLENQRLKQELLKSQTKVACLHSEMDLLKTELADQSINSERDEELMHQFSEERDILESQIEILQQANRKLHDTNDGLRTTLERISRSGNSESPVEMKSRNRSKSICYTSPYALIDRYCQRMNDNFCPYNHQPSSDTLALAMCDPGLMRRNSSECEEDSLPEFYMDSGLSTLRGSHGGYDSEQEVKGHEDEDEEEEEERGKKTVEDDRMMGENSDTEPAETQDTESAFGSDSSSVLDWKLPEITSDSKTSKPQTRALSAISVQKDEDNVNRGYMTSEKAFRIVLAGDAAVGKSSFLLRLCKNEFMPSSSATLGVDFQIKTLVVDGDPVLLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCERSFLNVREWVDMIEDLSPDNISIMLVGNKCDLRQDGANFVPTSYGEKLAMTYNTLFCETSAKDGSNILEAVLHLARQVKQYASLNKKADHQSLPSLDAPRKKPNLSCCT

Target ORF information:

RefSeq Version	XM_008337673.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis RAS and EF-hand domain containing (rasef), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008337673.2

ORF Insert Sequence:

ATGGGAATAG AGGAGTTGCA GGTGGGCAGG TTGAAGGTGG TGTTGGTACC GGCAGAGTGG 
AAGGAGGGAG GTGTGATGTT GTGGAGCTTG GGTGGTAGGC CACTGAGAGA GGGAGTGGAG 
GAGGCTGCAA TGAGGATGTA TCCAGGCCTG AAGCATCTCA ACATTGGACA AGTGCCTAAA 
GTCAAGGTGC CCAGCCTCAC AGTCATGATG TCACACCTTA AATTTCTCCT GACTCTAAAT 
CTCACTGGTC TTCAGGCAAT CTCTGGATTG ACCATTGAGG ATTTGACCAG CAGCTCAGAA 
GAACAGCACA AAGATAAGGA AGATAAGTGC AAGGCGGAGG ACACCTTCAC TGAGAAGATT 
ACCAACAAGC AGCTGAAGTT GTCAAGTCAG CCTTATGTCT CCATCAGTGG CGTGAGCTCA 
GAGGAGCGTG ATCGGCTACG CTCTCTCTTT CACGCCTACG ACGTGAACAA TTCTGGGAGG 
ATCGAAAGAC GGCAGTTCTT CACCATCTGT GCGCTGCTCC AAGTGTCCAC AGCCGCGGCG 
CAGAGCGTGT TTGACAGGCT TGACGTGGGC GAGGACGGCA CGGTCACCGT GCTGGAGTTT 
ATCAGCGGCT TCTACGATCG CTACGGTGAA GACATGGAGA GTGACGGAGC AGAGGTCTGG 
TCTGCTGCCT GGGAAGACTT CTCCGGCAGA CTCGGGGCAC AGGCCAAGTT CATCCCCAGA 
AATGAGCAAG CAGCAATGTT GTACCAGAAC ATCAGCCTGA CAGAACCCAG ACTGATACCC 
CAGTTTGAAA AAGTCATCCT GAACTTCACC AAAGAGATCA GGCAGCAAAA CCTGGAGATG 
GAGAACCTGG CTCTGGCTAT CAAAAGGGCA CAGGACCAGG CATCTATGCA ACTGAGTGAG 
ATGGAGGAGG AGATGGACCA GCGCATCCAG GCTGCAGAGA AAACAACACG AGAACAGGAG 
AAGATGCGAG CAGAGGCAGA ACTGAGTGAG ATACGGAGAG ACTTTGAAAC TGAAGTGTGT 
GAACTTCAGT GTAAGATCCA GAAGATGCAG TTGATTGAAG AGAAGTACAT GAACATCACA 
GTGAAAGACC AGTCTCTGAC ATTAAAGAAG ACGATCTCTG AGATGACGCT GGAAAACCAG 
CGACTGAAGC AGGAGCTGCT GAAATCTCAG ACCAAAGTCG CCTGTTTACA CAGTGAGATG 
GACTTACTGA AGACCGAGTT AGCTGATCAA AGTATCAACT CTGAAAGAGA TGAGGAGCTC 
ATGCACCAGT TCTCTGAAGA ACGTGACATT CTGGAGAGTC AGATTGAGAT TTTGCAACAA 
GCTAACAGAA AGCTTCATGA CACTAATGAT GGTCTGAGGA CAACTCTGGA AAGGATTTCA 
AGGTCTGGTA ACAGTGAGTC CCCAGTAGAA ATGAAGAGCA GGAACAGGAG TAAGAGCATC 
TGTTACACAT CGCCGTATGC GTTAATAGAC AGGTACTGCC AGCGTATGAA CGACAACTTC 
TGTCCGTACA ACCATCAACC CAGCTCCGAC ACACTGGCCT TGGCCATGTG TGACCCCGGC 
CTGATGCGCA GGAACAGCAG TGAGTGTGAA GAGGACAGTC TGCCTGAATT CTACATGGAC 
AGCGGCCTGT CCACACTCAG GGGCTCCCAT GGTGGTTACG ACTCCGAGCA GGAGGTTAAA 
GGTCACGAAG ATGAAGACGA AGAGGAGGAG GAAGAGAGGG GGAAGAAGAC AGTGGAGGAT 
GACAGAATGA TGGGAGAAAA CTCAGATACT GAGCCAGCAG AGACACAGGA CACCGAATCT 
GCATTTGGTT CGGACAGCAG CTCAGTGTTG GACTGGAAAC TGCCTGAGAT CACTAGTGAC 
AGCAAAACAT CGAAACCCCA AACGAGGGCT CTCAGTGCCA TCAGTGTTCA GAAGGATGAA 
GATAATGTTA ACCGGGGCTA CATGACGTCA GAGAAGGCCT TCAGAATCGT TTTGGCAGGA 
GACGCTGCTG TGGGAAAGTC CAGCTTCCTG CTGCGCCTTT GTAAGAATGA ATTTATGCCA 
AGCTCCAGTG CAACCTTGGG AGTGGATTTC CAGATCAAGA CACTGGTAGT TGATGGAGAT 
CCTGTTCTAC TTCAGTTATG GGACACTGCA GGACAGGAAA GGTTCCGCAG CATTGCTAAG 
TCATATTTCC GTCGAGCGGA CGGTGTTTTG CTGCTGTATG ACGTCACCTG TGAGAGAAGT 
TTCCTTAACG TGAGAGAGTG GGTGGATATG ATAGAGGACC TATCTCCAGA CAATATTTCC 
ATCATGCTCG TGGGCAATAA GTGTGACCTG AGACAAGACG GAGCTAACTT TGTTCCTACC 
AGCTATGGTG AAAAGCTGGC CATGACATAC AACACTCTGT TCTGTGAGAC TAGTGCCAAA 
GATGGTTCCA ACATCCTGGA GGCTGTGTTG CATTTGGCCA GGCAAGTCAA ACAGTACGCT 
TCTTTAAATA AAAAGGCTGA CCATCAGTCC CTGCCCAGTC TGGATGCTCC CAGGAAGAAA 
CCAAACCTCT CCTGCTGCAC CTGA