bbs9 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	bbs9
Entrez Gene ID	103388686
Full Name	Bardet-Biedl syndrome 9
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008323797.3	XP_008322019.1	protein PTHB1
XM_008323797.2	XP_008322019.1	protein PTHB1

Pathways from BioSystems
Gene Ontology

The following bbs9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bbs9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy41196	XM_008323797.3 Latest version!	Cynoglossus semilaevis Bardet-Biedl syndrome 9 (bbs9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OCy41196	XM_008323797.2	Cynoglossus semilaevis Bardet-Biedl syndrome 9 (bbs9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bbs9 ( XM_008323797.3 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008322019.1 Cynoglossus semilaevis Bardet-Biedl syndrome 9 (bbs9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCy41196
Clone ID Related Accession (Same CDS sequence)	XM_008323797.3 , XM_008323797.2
Accession Version	XM_008323797.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	protein PTHB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024319.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_008323797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGT TCAAAGCCCG CGACTGGTGG TCGGCATCCC TGGGCGAGGG GGAGGAGTTT 
GACCAGGGAT GTCTGTGCGT TGGCAATGTA GACAACAGTG GAAATGGACA CGACAAAGTT 
GTGGTGGGCA GCTACATGGG AATGTTACGG ATCTTTGCAC CTCAGGCCAG TAAGTCAGGT 
GAGGGAGGAG TCACGGACAC CCAGCTCCTG GAGGTCCAGC TTCAGAACGC CATCATCCAG 
GTGGAAGTGG GCAAGTTTGT CTCATGCTCA GATCTTCTCC ACTTGGCTGT TCTCCACCCC 
AGGAAACTGT CAGTGTACTC TGTCTCAGGC ACTGCAGGAA ACGTGGAACA TGGTGACCAG 
TACCAGCTGA AGCTGGTTTA CGAACACAAT CTGAAGAGAA CAGCCTGCAG TATGACCTAC 
GGCACCTTTG GTGGAGTAAC TGGTCACCAC TCGGTGTGTA TCCAGTCTAT GGATGGGATG 
CTGATGTTCT TTGAACAGGA CAGTTACTCC TTTGGCCGAT TCCTACCTGG TTTCCTGCTC 
CCAGGACCAC TGGTCTACAA CACCAGAACC GACAGCTTTC TCACCGTGTC CTCTGCACGA 
CAACTGGAGA GTTACAAATA TGAGACCTTG GCTGTGGCCA CAGAAGCAGA GAGTCGTCAG 
GATCCAGACC TGCCTCCAAA GACCGGAGGC AAGAAGCTGA CGCCCGACTG GACGTTCATC 
CTGGGAGAAC AGGCCCTGGA CCTCTCCGTT CCTTCCTTCT CCCACTCTTC TGCCTCTATC 
TTCATCTTGG GTGAAAGGAA CCTTTACTGT CTCAGAGACA ACGGCCAGAT TCGCTTCATG 
AAGAAACTGG AGTACAACCC CAGCTGCTTT CTTCCCTACG CTTCAGTGAT GGACGGCACC 
ACCAACCTGT TGTTGGGCAA CCATACAAAT ATGCTGCTGG TCTACCAGGA TGTGACTCTA 
AAGTGGGCAG CCCAGCTCGC CTTCGTGCCC GTGGCCGTCC GAGTTGCCAA CTTCCCGGAG 
TTGAAGGGAG TGGTGGTCAG TCTGAGTTCA GATGGACACC TCCTGTGCTC CTACATGGGC 
ACCGATCCCT CTTTCTTCTC CACCCCAAAG GTGGACGCCA GAGAGGCAGA CTATGAGCAG 
GTAGATGCTG AGATGAAGAA GCTGCAGAAG TTCATCAGAG AGGCTACGAG AACCCAGGAC 
ATCCTCCCTA AATCTGATGC AGAGACGGAC CTTACTGTCA AAGCTGCCGT GTCCTCCAGC 
ATGGACACTC CGTCGCAAGC TGTGATTCCA GATACAGATG GTCTGTCTGT ACCTTCAGTA 
ACTGTACAGG TGAAGATAAA GGCCAGCTGT GTTCTTCAGG CTTCAAAGTT GATGGTCAGC 
GTTCAGGCTC CACTGGCCGT CACCAAGGAC CAGTTTGTCC TGGAGTCCAT GGGTGTTGGC 
TCCTCCACAG TCGTGTCTTT TTCAGTTTTC CTCAATGGCC GTTATCCTCC TGCTGATCTC 
AGTGGAGACA TCACTGTGTC CTACAGCTCA CCCACAGGAG TTCCCCGGGT TCTCCAGTCC 
AAGTTCAGTC TTCCTCTCCT GTTGGTGTGT ACTCCTTCCC CTCCGGCCAA AACCAGCAAG 
TTCAAGATCA GCGTGGACAC AAACCAGCCG CCGGTCAACC TCAACACCAT CTTCCCAGAG 
TTTTTAGTGA AATCCGAAGA TAAGGATGGG AATGCTGTGG CGTTCCAGTT CCTGTCAGGA 
GCCAGTGTTA CAGTGGTGGC CTCTAAGACC TCCCAGCGTT ACCGTGTCCA GAGCGACAGC 
TTTGAGGACA TGTGGTTAGT GGTGAAGGAG CTGCTTCACA GGTTTGACCA GCATTTCTCC 
AAACTGGGAG TCAAGGACTT CAGGAAAAGC TTCAGCGGAC CTGTCCCACT TCAGGACTAC 
TTCCTGTCTG TGGACAACCA TTTCCAGCTG CGCCTCACTG CCCAGCAGTA CCAGGACCTC 
CTGTCAGAAC GGGCGGTCCA GTTTAGGGCC ATTCAGCGGC GCCTGCTGAC TCGGTTCAAG 
GACAAAACCC CGGCACCACT ACAGAACCTG GACACACTGC TGGATGCCAC GTACAGTCAG 
GTCATAACAC TGGCAGAGGC TGCAGAAGAG AACCACACAC TCCTGGAGGA GGCGTTCGTC 
CGTCTGAGGA GCGCCACACA CCTGCTGGTG CTGCTGCTGT CACTGTGGCA GAACCTGACC 
CCGGACCAGA GCTCCATCCT GGAGGCCGCA CTGCTGCCAC TGCTGCAGGA CACACCACAG 
CTGGGCTGGG AGGAGAGCTG CGACGCAGCC GTCACTCACC TGCTGCGAAC TTGCCTTTCC 
CGCAGTCCCA AAGACCAGGC TGCATCTCTG GCCCAGATTG GTAGCCCCCT GCTGGGTCTC 
CCCCGTGACA CCACCCGCCT AAAAAAGCAC ATCACACTGC TGTGCGAACG CATAAGCAAG 
GGAGGACAGC TGACTCTGGC CTCGGAGACT AATGTCCAGG TCCCTGCCCA CATCCAGAAC 
CTGGCTGCAC CTGGAGGAGT TGAGCCCATT GCAGAAGGAG CTGGTGAAGG TGAGGAGCTA 
GAACTTCCCC AGGATGCCAC AAAGTTCCTG AAGAAGAAAC AGCCGTCTAA GAAGGTCAAG 
AAAGTCAAAG AAAAGGAGAA AAGGGAGGTG TCCAGGGAGC AACCAGAAGA AACAAGAGGA 
GCAGAGGAGC CAGTCAAAGA GGCCCGAAGG GAAAAGAAGG AGTCAAGGAG CAAAGAGGAG 
CCAAAGAAGG AGTCCAAGAA GGAGTCATCA TCCTCCAAGG AGAAAAAGGA GTCTTCAAAG 
GAAAAGAAGG ACAAAGACAG AGAAGGGAAG GAGACTAGTA AGGAAGCAGT GGAGAAGAAG 
GCCTCCAGGA AATCTTCAGT GAAAGTGAAG GAGATGAAGA ACGAGCCGCA TGCTGAAGAC 
GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008322019.1
CDS	826..3771
Translation	MSLFKARDWWSASLGEGEEFDQGCLCVGNVDNSGNGHDKVVVGSYMGMLRIFAPQASKSGEGGVTDTQLLEVQLQNAIIQVEVGKFVSCSDLLHLAVLHPRKLSVYSVSGTAGNVEHGDQYQLKLVYEHNLKRTACSMTYGTFGGVTGHHSVCIQSMDGMLMFFEQDSYSFGRFLPGFLLPGPLVYNTRTDSFLTVSSARQLESYKYETLAVATEAESRQDPDLPPKTGGKKLTPDWTFILGEQALDLSVPSFSHSSASIFILGERNLYCLRDNGQIRFMKKLEYNPSCFLPYASVMDGTTNLLLGNHTNMLLVYQDVTLKWAAQLAFVPVAVRVANFPELKGVVVSLSSDGHLLCSYMGTDPSFFSTPKVDAREADYEQVDAEMKKLQKFIREATRTQDILPKSDAETDLTVKAAVSSSMDTPSQAVIPDTDGLSVPSVTVQVKIKASCVLQASKLMVSVQAPLAVTKDQFVLESMGVGSSTVVSFSVFLNGRYPPADLSGDITVSYSSPTGVPRVLQSKFSLPLLLVCTPSPPAKTSKFKISVDTNQPPVNLNTIFPEFLVKSEDKDGNAVAFQFLSGASVTVVASKTSQRYRVQSDSFEDMWLVVKELLHRFDQHFSKLGVKDFRKSFSGPVPLQDYFLSVDNHFQLRLTAQQYQDLLSERAVQFRAIQRRLLTRFKDKTPAPLQNLDTLLDATYSQVITLAEAAEENHTLLEEAFVRLRSATHLLVLLLSLWQNLTPDQSSILEAALLPLLQDTPQLGWEESCDAAVTHLLRTCLSRSPKDQAASLAQIGSPLLGLPRDTTRLKKHITLLCERISKGGQLTLASETNVQVPAHIQNLAAPGGVEPIAEGAGEGEELELPQDATKFLKKKQPSKKVKKVKEKEKREVSREQPEETRGAEEPVKEARREKKESRSKEEPKKESKKESSSSKEKKESSKEKKDKDREGKETSKEAVEKKASRKSSVKVKEMKNEPHAEDG

Target ORF information:

RefSeq Version	XM_008323797.3
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis Bardet-Biedl syndrome 9 (bbs9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008323797.3

ORF Insert Sequence:

ATGTCTCTGT TCAAAGCCCG CGACTGGTGG TCGGCATCCC TGGGCGAGGG GGAGGAGTTT 
GACCAGGGAT GTCTGTGCGT TGGCAATGTA GACAACAGTG GAAATGGACA CGACAAAGTT 
GTGGTGGGCA GCTACATGGG AATGTTACGG ATCTTTGCAC CTCAGGCCAG TAAGTCAGGT 
GAGGGAGGAG TCACGGACAC CCAGCTCCTG GAGGTCCAGC TTCAGAACGC CATCATCCAG 
GTGGAAGTGG GCAAGTTTGT CTCATGCTCA GATCTTCTCC ACTTGGCTGT TCTCCACCCC 
AGGAAACTGT CAGTGTACTC TGTCTCAGGC ACTGCAGGAA ACGTGGAACA TGGTGACCAG 
TACCAGCTGA AGCTGGTTTA CGAACACAAT CTGAAGAGAA CAGCCTGCAG TATGACCTAC 
GGCACCTTTG GTGGAGTAAC TGGTCACCAC TCGGTGTGTA TCCAGTCTAT GGATGGGATG 
CTGATGTTCT TTGAACAGGA CAGTTACTCC TTTGGCCGAT TCCTACCTGG TTTCCTGCTC 
CCAGGACCAC TGGTCTACAA CACCAGAACC GACAGCTTTC TCACCGTGTC CTCTGCACGA 
CAACTGGAGA GTTACAAATA TGAGACCTTG GCTGTGGCCA CAGAAGCAGA GAGTCGTCAG 
GATCCAGACC TGCCTCCAAA GACCGGAGGC AAGAAGCTGA CGCCCGACTG GACGTTCATC 
CTGGGAGAAC AGGCCCTGGA CCTCTCCGTT CCTTCCTTCT CCCACTCTTC TGCCTCTATC 
TTCATCTTGG GTGAAAGGAA CCTTTACTGT CTCAGAGACA ACGGCCAGAT TCGCTTCATG 
AAGAAACTGG AGTACAACCC CAGCTGCTTT CTTCCCTACG CTTCAGTGAT GGACGGCACC 
ACCAACCTGT TGTTGGGCAA CCATACAAAT ATGCTGCTGG TCTACCAGGA TGTGACTCTA 
AAGTGGGCAG CCCAGCTCGC CTTCGTGCCC GTGGCCGTCC GAGTTGCCAA CTTCCCGGAG 
TTGAAGGGAG TGGTGGTCAG TCTGAGTTCA GATGGACACC TCCTGTGCTC CTACATGGGC 
ACCGATCCCT CTTTCTTCTC CACCCCAAAG GTGGACGCCA GAGAGGCAGA CTATGAGCAG 
GTAGATGCTG AGATGAAGAA GCTGCAGAAG TTCATCAGAG AGGCTACGAG AACCCAGGAC 
ATCCTCCCTA AATCTGATGC AGAGACGGAC CTTACTGTCA AAGCTGCCGT GTCCTCCAGC 
ATGGACACTC CGTCGCAAGC TGTGATTCCA GATACAGATG GTCTGTCTGT ACCTTCAGTA 
ACTGTACAGG TGAAGATAAA GGCCAGCTGT GTTCTTCAGG CTTCAAAGTT GATGGTCAGC 
GTTCAGGCTC CACTGGCCGT CACCAAGGAC CAGTTTGTCC TGGAGTCCAT GGGTGTTGGC 
TCCTCCACAG TCGTGTCTTT TTCAGTTTTC CTCAATGGCC GTTATCCTCC TGCTGATCTC 
AGTGGAGACA TCACTGTGTC CTACAGCTCA CCCACAGGAG TTCCCCGGGT TCTCCAGTCC 
AAGTTCAGTC TTCCTCTCCT GTTGGTGTGT ACTCCTTCCC CTCCGGCCAA AACCAGCAAG 
TTCAAGATCA GCGTGGACAC AAACCAGCCG CCGGTCAACC TCAACACCAT CTTCCCAGAG 
TTTTTAGTGA AATCCGAAGA TAAGGATGGG AATGCTGTGG CGTTCCAGTT CCTGTCAGGA 
GCCAGTGTTA CAGTGGTGGC CTCTAAGACC TCCCAGCGTT ACCGTGTCCA GAGCGACAGC 
TTTGAGGACA TGTGGTTAGT GGTGAAGGAG CTGCTTCACA GGTTTGACCA GCATTTCTCC 
AAACTGGGAG TCAAGGACTT CAGGAAAAGC TTCAGCGGAC CTGTCCCACT TCAGGACTAC 
TTCCTGTCTG TGGACAACCA TTTCCAGCTG CGCCTCACTG CCCAGCAGTA CCAGGACCTC 
CTGTCAGAAC GGGCGGTCCA GTTTAGGGCC ATTCAGCGGC GCCTGCTGAC TCGGTTCAAG 
GACAAAACCC CGGCACCACT ACAGAACCTG GACACACTGC TGGATGCCAC GTACAGTCAG 
GTCATAACAC TGGCAGAGGC TGCAGAAGAG AACCACACAC TCCTGGAGGA GGCGTTCGTC 
CGTCTGAGGA GCGCCACACA CCTGCTGGTG CTGCTGCTGT CACTGTGGCA GAACCTGACC 
CCGGACCAGA GCTCCATCCT GGAGGCCGCA CTGCTGCCAC TGCTGCAGGA CACACCACAG 
CTGGGCTGGG AGGAGAGCTG CGACGCAGCC GTCACTCACC TGCTGCGAAC TTGCCTTTCC 
CGCAGTCCCA AAGACCAGGC TGCATCTCTG GCCCAGATTG GTAGCCCCCT GCTGGGTCTC 
CCCCGTGACA CCACCCGCCT AAAAAAGCAC ATCACACTGC TGTGCGAACG CATAAGCAAG 
GGAGGACAGC TGACTCTGGC CTCGGAGACT AATGTCCAGG TCCCTGCCCA CATCCAGAAC 
CTGGCTGCAC CTGGAGGAGT TGAGCCCATT GCAGAAGGAG CTGGTGAAGG TGAGGAGCTA 
GAACTTCCCC AGGATGCCAC AAAGTTCCTG AAGAAGAAAC AGCCGTCTAA GAAGGTCAAG 
AAAGTCAAAG AAAAGGAGAA AAGGGAGGTG TCCAGGGAGC AACCAGAAGA AACAAGAGGA 
GCAGAGGAGC CAGTCAAAGA GGCCCGAAGG GAAAAGAAGG AGTCAAGGAG CAAAGAGGAG 
CCAAAGAAGG AGTCCAAGAA GGAGTCATCA TCCTCCAAGG AGAAAAAGGA GTCTTCAAAG 
GAAAAGAAGG ACAAAGACAG AGAAGGGAAG GAGACTAGTA AGGAAGCAGT GGAGAAGAAG 
GCCTCCAGGA AATCTTCAGT GAAAGTGAAG GAGATGAAGA ACGAGCCGCA TGCTGAAGAC 
GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbs9 ( XM_008323797.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008322019.1 Cynoglossus semilaevis Bardet-Biedl syndrome 9 (bbs9), mRNA.

Price & Availability↑

CloneID	OCy41196
Clone ID Related Accession (Same CDS sequence)	XM_008323797.3 , XM_008323797.2
Accession Version	XM_008323797.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	protein PTHB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024319.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008323797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGT TCAAAGCCCG CGACTGGTGG TCGGCATCCC TGGGCGAGGG GGAGGAGTTT 
GACCAGGGAT GTCTGTGCGT TGGCAATGTA GACAACAGTG GAAATGGACA CGACAAAGTT 
GTGGTGGGCA GCTACATGGG AATGTTACGG ATCTTTGCAC CTCAGGCCAG TAAGTCAGGT 
GAGGGAGGAG TCACGGACAC CCAGCTCCTG GAGGTCCAGC TTCAGAACGC CATCATCCAG 
GTGGAAGTGG GCAAGTTTGT CTCATGCTCA GATCTTCTCC ACTTGGCTGT TCTCCACCCC 
AGGAAACTGT CAGTGTACTC TGTCTCAGGC ACTGCAGGAA ACGTGGAACA TGGTGACCAG 
TACCAGCTGA AGCTGGTTTA CGAACACAAT CTGAAGAGAA CAGCCTGCAG TATGACCTAC 
GGCACCTTTG GTGGAGTAAC TGGTCACCAC TCGGTGTGTA TCCAGTCTAT GGATGGGATG 
CTGATGTTCT TTGAACAGGA CAGTTACTCC TTTGGCCGAT TCCTACCTGG TTTCCTGCTC 
CCAGGACCAC TGGTCTACAA CACCAGAACC GACAGCTTTC TCACCGTGTC CTCTGCACGA 
CAACTGGAGA GTTACAAATA TGAGACCTTG GCTGTGGCCA CAGAAGCAGA GAGTCGTCAG 
GATCCAGACC TGCCTCCAAA GACCGGAGGC AAGAAGCTGA CGCCCGACTG GACGTTCATC 
CTGGGAGAAC AGGCCCTGGA CCTCTCCGTT CCTTCCTTCT CCCACTCTTC TGCCTCTATC 
TTCATCTTGG GTGAAAGGAA CCTTTACTGT CTCAGAGACA ACGGCCAGAT TCGCTTCATG 
AAGAAACTGG AGTACAACCC CAGCTGCTTT CTTCCCTACG CTTCAGTGAT GGACGGCACC 
ACCAACCTGT TGTTGGGCAA CCATACAAAT ATGCTGCTGG TCTACCAGGA TGTGACTCTA 
AAGTGGGCAG CCCAGCTCGC CTTCGTGCCC GTGGCCGTCC GAGTTGCCAA CTTCCCGGAG 
TTGAAGGGAG TGGTGGTCAG TCTGAGTTCA GATGGACACC TCCTGTGCTC CTACATGGGC 
ACCGATCCCT CTTTCTTCTC CACCCCAAAG GTGGACGCCA GAGAGGCAGA CTATGAGCAG 
GTAGATGCTG AGATGAAGAA GCTGCAGAAG TTCATCAGAG AGGCTACGAG AACCCAGGAC 
ATCCTCCCTA AATCTGATGC AGAGACGGAC CTTACTGTCA AAGCTGCCGT GTCCTCCAGC 
ATGGACACTC CGTCGCAAGC TGTGATTCCA GATACAGATG GTCTGTCTGT ACCTTCAGTA 
ACTGTACAGG TGAAGATAAA GGCCAGCTGT GTTCTTCAGG CTTCAAAGTT GATGGTCAGC 
GTTCAGGCTC CACTGGCCGT CACCAAGGAC CAGTTTGTCC TGGAGTCCAT GGGTGTTGGC 
TCCTCCACAG TCGTGTCTTT TTCAGTTTTC CTCAATGGCC GTTATCCTCC TGCTGATCTC 
AGTGGAGACA TCACTGTGTC CTACAGCTCA CCCACAGGAG TTCCCCGGGT TCTCCAGTCC 
AAGTTCAGTC TTCCTCTCCT GTTGGTGTGT ACTCCTTCCC CTCCGGCCAA AACCAGCAAG 
TTCAAGATCA GCGTGGACAC AAACCAGCCG CCGGTCAACC TCAACACCAT CTTCCCAGAG 
TTTTTAGTGA AATCCGAAGA TAAGGATGGG AATGCTGTGG CGTTCCAGTT CCTGTCAGGA 
GCCAGTGTTA CAGTGGTGGC CTCTAAGACC TCCCAGCGTT ACCGTGTCCA GAGCGACAGC 
TTTGAGGACA TGTGGTTAGT GGTGAAGGAG CTGCTTCACA GGTTTGACCA GCATTTCTCC 
AAACTGGGAG TCAAGGACTT CAGGAAAAGC TTCAGCGGAC CTGTCCCACT TCAGGACTAC 
TTCCTGTCTG TGGACAACCA TTTCCAGCTG CGCCTCACTG CCCAGCAGTA CCAGGACCTC 
CTGTCAGAAC GGGCGGTCCA GTTTAGGGCC ATTCAGCGGC GCCTGCTGAC TCGGTTCAAG 
GACAAAACCC CGGCACCACT ACAGAACCTG GACACACTGC TGGATGCCAC GTACAGTCAG 
GTCATAACAC TGGCAGAGGC TGCAGAAGAG AACCACACAC TCCTGGAGGA GGCGTTCGTC 
CGTCTGAGGA GCGCCACACA CCTGCTGGTG CTGCTGCTGT CACTGTGGCA GAACCTGACC 
CCGGACCAGA GCTCCATCCT GGAGGCCGCA CTGCTGCCAC TGCTGCAGGA CACACCACAG 
CTGGGCTGGG AGGAGAGCTG CGACGCAGCC GTCACTCACC TGCTGCGAAC TTGCCTTTCC 
CGCAGTCCCA AAGACCAGGC TGCATCTCTG GCCCAGATTG GTAGCCCCCT GCTGGGTCTC 
CCCCGTGACA CCACCCGCCT AAAAAAGCAC ATCACACTGC TGTGCGAACG CATAAGCAAG 
GGAGGACAGC TGACTCTGGC CTCGGAGACT AATGTCCAGG TCCCTGCCCA CATCCAGAAC 
CTGGCTGCAC CTGGAGGAGT TGAGCCCATT GCAGAAGGAG CTGGTGAAGG TGAGGAGCTA 
GAACTTCCCC AGGATGCCAC AAAGTTCCTG AAGAAGAAAC AGCCGTCTAA GAAGGTCAAG 
AAAGTCAAAG AAAAGGAGAA AAGGGAGGTG TCCAGGGAGC AACCAGAAGA AACAAGAGGA 
GCAGAGGAGC CAGTCAAAGA GGCCCGAAGG GAAAAGAAGG AGTCAAGGAG CAAAGAGGAG 
CCAAAGAAGG AGTCCAAGAA GGAGTCATCA TCCTCCAAGG AGAAAAAGGA GTCTTCAAAG 
GAAAAGAAGG ACAAAGACAG AGAAGGGAAG GAGACTAGTA AGGAAGCAGT GGAGAAGAAG 
GCCTCCAGGA AATCTTCAGT GAAAGTGAAG GAGATGAAGA ACGAGCCGCA TGCTGAAGAC 
GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008322019.1
CDS	202..3147
Translation	MSLFKARDWWSASLGEGEEFDQGCLCVGNVDNSGNGHDKVVVGSYMGMLRIFAPQASKSGEGGVTDTQLLEVQLQNAIIQVEVGKFVSCSDLLHLAVLHPRKLSVYSVSGTAGNVEHGDQYQLKLVYEHNLKRTACSMTYGTFGGVTGHHSVCIQSMDGMLMFFEQDSYSFGRFLPGFLLPGPLVYNTRTDSFLTVSSARQLESYKYETLAVATEAESRQDPDLPPKTGGKKLTPDWTFILGEQALDLSVPSFSHSSASIFILGERNLYCLRDNGQIRFMKKLEYNPSCFLPYASVMDGTTNLLLGNHTNMLLVYQDVTLKWAAQLAFVPVAVRVANFPELKGVVVSLSSDGHLLCSYMGTDPSFFSTPKVDAREADYEQVDAEMKKLQKFIREATRTQDILPKSDAETDLTVKAAVSSSMDTPSQAVIPDTDGLSVPSVTVQVKIKASCVLQASKLMVSVQAPLAVTKDQFVLESMGVGSSTVVSFSVFLNGRYPPADLSGDITVSYSSPTGVPRVLQSKFSLPLLLVCTPSPPAKTSKFKISVDTNQPPVNLNTIFPEFLVKSEDKDGNAVAFQFLSGASVTVVASKTSQRYRVQSDSFEDMWLVVKELLHRFDQHFSKLGVKDFRKSFSGPVPLQDYFLSVDNHFQLRLTAQQYQDLLSERAVQFRAIQRRLLTRFKDKTPAPLQNLDTLLDATYSQVITLAEAAEENHTLLEEAFVRLRSATHLLVLLLSLWQNLTPDQSSILEAALLPLLQDTPQLGWEESCDAAVTHLLRTCLSRSPKDQAASLAQIGSPLLGLPRDTTRLKKHITLLCERISKGGQLTLASETNVQVPAHIQNLAAPGGVEPIAEGAGEGEELELPQDATKFLKKKQPSKKVKKVKEKEKREVSREQPEETRGAEEPVKEARREKKESRSKEEPKKESKKESSSSKEKKESSKEKKDKDREGKETSKEAVEKKASRKSSVKVKEMKNEPHAEDG

Target ORF information:

RefSeq Version	XM_008323797.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis Bardet-Biedl syndrome 9 (bbs9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008323797.2

ORF Insert Sequence:

ATGTCTCTGT TCAAAGCCCG CGACTGGTGG TCGGCATCCC TGGGCGAGGG GGAGGAGTTT 
GACCAGGGAT GTCTGTGCGT TGGCAATGTA GACAACAGTG GAAATGGACA CGACAAAGTT 
GTGGTGGGCA GCTACATGGG AATGTTACGG ATCTTTGCAC CTCAGGCCAG TAAGTCAGGT 
GAGGGAGGAG TCACGGACAC CCAGCTCCTG GAGGTCCAGC TTCAGAACGC CATCATCCAG 
GTGGAAGTGG GCAAGTTTGT CTCATGCTCA GATCTTCTCC ACTTGGCTGT TCTCCACCCC 
AGGAAACTGT CAGTGTACTC TGTCTCAGGC ACTGCAGGAA ACGTGGAACA TGGTGACCAG 
TACCAGCTGA AGCTGGTTTA CGAACACAAT CTGAAGAGAA CAGCCTGCAG TATGACCTAC 
GGCACCTTTG GTGGAGTAAC TGGTCACCAC TCGGTGTGTA TCCAGTCTAT GGATGGGATG 
CTGATGTTCT TTGAACAGGA CAGTTACTCC TTTGGCCGAT TCCTACCTGG TTTCCTGCTC 
CCAGGACCAC TGGTCTACAA CACCAGAACC GACAGCTTTC TCACCGTGTC CTCTGCACGA 
CAACTGGAGA GTTACAAATA TGAGACCTTG GCTGTGGCCA CAGAAGCAGA GAGTCGTCAG 
GATCCAGACC TGCCTCCAAA GACCGGAGGC AAGAAGCTGA CGCCCGACTG GACGTTCATC 
CTGGGAGAAC AGGCCCTGGA CCTCTCCGTT CCTTCCTTCT CCCACTCTTC TGCCTCTATC 
TTCATCTTGG GTGAAAGGAA CCTTTACTGT CTCAGAGACA ACGGCCAGAT TCGCTTCATG 
AAGAAACTGG AGTACAACCC CAGCTGCTTT CTTCCCTACG CTTCAGTGAT GGACGGCACC 
ACCAACCTGT TGTTGGGCAA CCATACAAAT ATGCTGCTGG TCTACCAGGA TGTGACTCTA 
AAGTGGGCAG CCCAGCTCGC CTTCGTGCCC GTGGCCGTCC GAGTTGCCAA CTTCCCGGAG 
TTGAAGGGAG TGGTGGTCAG TCTGAGTTCA GATGGACACC TCCTGTGCTC CTACATGGGC 
ACCGATCCCT CTTTCTTCTC CACCCCAAAG GTGGACGCCA GAGAGGCAGA CTATGAGCAG 
GTAGATGCTG AGATGAAGAA GCTGCAGAAG TTCATCAGAG AGGCTACGAG AACCCAGGAC 
ATCCTCCCTA AATCTGATGC AGAGACGGAC CTTACTGTCA AAGCTGCCGT GTCCTCCAGC 
ATGGACACTC CGTCGCAAGC TGTGATTCCA GATACAGATG GTCTGTCTGT ACCTTCAGTA 
ACTGTACAGG TGAAGATAAA GGCCAGCTGT GTTCTTCAGG CTTCAAAGTT GATGGTCAGC 
GTTCAGGCTC CACTGGCCGT CACCAAGGAC CAGTTTGTCC TGGAGTCCAT GGGTGTTGGC 
TCCTCCACAG TCGTGTCTTT TTCAGTTTTC CTCAATGGCC GTTATCCTCC TGCTGATCTC 
AGTGGAGACA TCACTGTGTC CTACAGCTCA CCCACAGGAG TTCCCCGGGT TCTCCAGTCC 
AAGTTCAGTC TTCCTCTCCT GTTGGTGTGT ACTCCTTCCC CTCCGGCCAA AACCAGCAAG 
TTCAAGATCA GCGTGGACAC AAACCAGCCG CCGGTCAACC TCAACACCAT CTTCCCAGAG 
TTTTTAGTGA AATCCGAAGA TAAGGATGGG AATGCTGTGG CGTTCCAGTT CCTGTCAGGA 
GCCAGTGTTA CAGTGGTGGC CTCTAAGACC TCCCAGCGTT ACCGTGTCCA GAGCGACAGC 
TTTGAGGACA TGTGGTTAGT GGTGAAGGAG CTGCTTCACA GGTTTGACCA GCATTTCTCC 
AAACTGGGAG TCAAGGACTT CAGGAAAAGC TTCAGCGGAC CTGTCCCACT TCAGGACTAC 
TTCCTGTCTG TGGACAACCA TTTCCAGCTG CGCCTCACTG CCCAGCAGTA CCAGGACCTC 
CTGTCAGAAC GGGCGGTCCA GTTTAGGGCC ATTCAGCGGC GCCTGCTGAC TCGGTTCAAG 
GACAAAACCC CGGCACCACT ACAGAACCTG GACACACTGC TGGATGCCAC GTACAGTCAG 
GTCATAACAC TGGCAGAGGC TGCAGAAGAG AACCACACAC TCCTGGAGGA GGCGTTCGTC 
CGTCTGAGGA GCGCCACACA CCTGCTGGTG CTGCTGCTGT CACTGTGGCA GAACCTGACC 
CCGGACCAGA GCTCCATCCT GGAGGCCGCA CTGCTGCCAC TGCTGCAGGA CACACCACAG 
CTGGGCTGGG AGGAGAGCTG CGACGCAGCC GTCACTCACC TGCTGCGAAC TTGCCTTTCC 
CGCAGTCCCA AAGACCAGGC TGCATCTCTG GCCCAGATTG GTAGCCCCCT GCTGGGTCTC 
CCCCGTGACA CCACCCGCCT AAAAAAGCAC ATCACACTGC TGTGCGAACG CATAAGCAAG 
GGAGGACAGC TGACTCTGGC CTCGGAGACT AATGTCCAGG TCCCTGCCCA CATCCAGAAC 
CTGGCTGCAC CTGGAGGAGT TGAGCCCATT GCAGAAGGAG CTGGTGAAGG TGAGGAGCTA 
GAACTTCCCC AGGATGCCAC AAAGTTCCTG AAGAAGAAAC AGCCGTCTAA GAAGGTCAAG 
AAAGTCAAAG AAAAGGAGAA AAGGGAGGTG TCCAGGGAGC AACCAGAAGA AACAAGAGGA 
GCAGAGGAGC CAGTCAAAGA GGCCCGAAGG GAAAAGAAGG AGTCAAGGAG CAAAGAGGAG 
CCAAAGAAGG AGTCCAAGAA GGAGTCATCA TCCTCCAAGG AGAAAAAGGA GTCTTCAAAG 
GAAAAGAAGG ACAAAGACAG AGAAGGGAAG GAGACTAGTA AGGAAGCAGT GGAGAAGAAG 
GCCTCCAGGA AATCTTCAGT GAAAGTGAAG GAGATGAAGA ACGAGCCGCA TGCTGAAGAC 
GGTTGA