crim1 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	103387193
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008321765.2	XP_008319987.1	cysteine-rich motor neuron 1 protein
XM_008321765.3	XP_008319987.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy35238	XM_008321765.3 Latest version!	Cynoglossus semilaevis cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OCy35238	XM_008321765.2	Cynoglossus semilaevis cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_008321765.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008319987.1 Cynoglossus semilaevis cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.

Price & Availability↑

CloneID	OCy35238
Clone ID Related Accession (Same CDS sequence)	XM_008321765.2 , XM_008321765.3
Accession Version	XM_008321765.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024307.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008321765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA AGTGTCCTGT CCTCCTCCTC CTGAACGTCC TGTTTCTGGT CCGCGCTCTC 
TCCGCTCATG AGTGCACGCC GTGTCATCTG CGGACTTGTC CGCTGAAAGC CGCTCACGGC 
TGCGAAGGTG GTCGGACTCC GGTGAAGGAT CCGTGCGGAT GCTGCGACCA GTGCGCCCGC 
CTGGAGTGGG AGCCCTGCGG CGGACAGGAC TGGACGCACG GCTACTGCGC CCTCGGGCTA 
ACGTGTGCCT CCTTCAACAG CACCGAGGCT GCCACAATCC CGGAGATCGG AGTATGTAAA 
GTACTCCCTG ACTACCCAGA GGCTGGCCTC GAAGACGAGC GCTGCCCCCT AGTAACGGGC 
TGTGACACAG CAGGAGGACA GTGCGTGTGT GACGCCCGTC ACTCCTGTCT GGGCTCCTTC 
ACTTACCCCG ACCAAGAAAC CTGTATGAAG TCATTCAAGT CAGAGGGTCG GAGGCACGAG 
CACAGGGAGA GACACAGAGA GAAGTATGTG GGACCGTCCA ACCCGGCCTG CATGTTCTCG 
GGCTGTAACC TGACCACCGA GGGCTGTGTG TGCGAGTCTC AGAGCTGCCA TCACCACTTT 
GCCTACATCA ACCGCAGCCA GTGCCAGGAA GCCGCAGAGG AGCTGAGGTG TGCCCGGGTG 
ACATGCCCTG CTGTGCAGGT GCCCTCCTGC CCCCATGGTT CAGTGCTAAC CAAGAGTTAC 
ACGCCCCCTG CTGGCTGCTG TCCATCCATA CATCCCCAGT GCACATGCGA CTTCCACGCT 
TGCCCTAAGC CCCAGTGCCC CGGCGGGCAG AAAGCCGTGC GGCTCACTCG GGCCAGTGGT 
CAGCCGGGAG ACTGCTGTGA CGTCTTCGAG TGCCAAAAAG TCTCTCCCAA GTGCGTCCAC 
AACGAGAAGG AGTTCTCAGA GGGTGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGTCGAGGAG GAATCTCCTT CTGCTCCAAA GCCGAGTGTG CGGAGCTGGA CTGTGAAAAC 
TTCTACATTC CTGAGGGGGA GTGCTGCCCT GTCTGCATAG ATGTGGAGCT GCTGTCCATG 
GACAGCACAA AGGCCAGCTG CTGGGTGAAT AACAAGCTCC GGGCACATGA GGAGCAGTGG 
AAAGAGGATG ACTGCACCTT CTGCCAGTGT GTGGACGGGG ATCCACACTG CACGGCCATG 
GCCTGCAAAC AGAGCTGTCA AAACCCTGTC AAGATCCCCG GAGAATGCTG CCCCTTCTGT 
GAAGAGCCTT CGTACGAGAC AGTCAGCCCC TTGCTGTGTC CCCCTCTGGA GAACTGCTCC 
CTATCAGGGA ACGAGTGTCC CTATGGCTTT GAACAAGACA CAAACGGATG TCTCCTCTGT 
CAGTGTCTCG GCAATGACTC CTGCCCCGAA CTGGGAAAAT ACTGTTCCCT GCAATGCCCG 
ATGGGATACG AGAGGGACGA TTTTGGCTGT GAGGTGTGCG AGTGCAGCAT CCCCACGCCG 
AGGTGCCGAC CTCTGACCTG CAGTAAAACC TGCCCTTATG GATATGCGAG GAACAAGCAC 
GGCTGCGAAA TGTGCCGCTG TGTCAAGTGC CCTCCCTTCA CCTGCGACAA GCACTGCATC 
AACGGCTACC AGCAGAACAA GAAGGGTTGC TCCGTCTGCA TGTGCAAAGA GGGTAGCCCC 
ATTCCAAGGA CCACCCCCAC GCTGCCCGTA CCCAAGTACT GCCTCACCTC CAACGGTCAC 
AGATATGAGG AAGATGAAGG ATGGCATGAC GGATGTCGTG ACTGCTACTG TCACTCTGGA 
CGGGAGATGT GTGTTCTTAT TACCTGTCCT GTGCCCAACT GTCCTCAACC AGTGGTCAGG 
CCTGACCAGT GCTGTCCCAC ATGCGAAGAT GAGTCAGGCA GTGGTCAACC AGATGGGATG 
GACATGGTGG TGTGCAGAGC ACCAGGGGGG GAGTTTTACG TGGAGGGGGA GACCTGGAAC 
TTGGACGAGT GCACCAGCTG CACCTGCCGG GGGGGTCGCG TCCTGTGCGA CACGGAGGTG 
TGTCCACCAG CTCTGTGTCA GACCCCCACC AGAAACAAGG ACACCTGCTG CCACGTATGC 
CCAGAGGAGA GCCCACTGCT ACCTGTCAAC AACAACGAGC AGGAGTACTG CATCACCAAT 
GACGGAGACG TGCTACTGAA CGGAGACTCC TGGAAGGCGA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGCTTCTCT CAGCACTGCC CAGCTGCCAA CTGCAGGGTG 
CCCGTCTTAC GCAAAGGCCA GTGCTGCCCA TACTGTATTG ATATGACAAC GTTGGTGCCA 
GTGACGGTGT CCACCAGTGG TCGTGCTGCT ACAGTGAAGA GTGCAGTGTT GACAACCACT 
GTGTCACCAC TGCCGCCCAT CATCGCAGAT ACAGATGAGC CAGGTTTGGG CGAGAACTAT 
CCCAGCCACA CAGAGATGAC GCTCATCTAC CAATCAGCTG CCTGGATCCT GGCTGGTCTG 
CTTCTGGCCA TCATCATCTT CCTCATTGTA GCCTTGCTCA TCAACAAGAA AAAGAAGTGG 
GTTCAGATGT CGTGCTACAG TGCACCAAAG AAGACGGTAA TCCTGAAAAA GCACGTAAAC 
AAGAACTCGG TGGTCTACAT GGAGCCGTCC AAGGAGAACA AATTCCAAAA CGTGAAGAAC 
GACTGTGGCA TCATTCACTT CTCTCCCGAC ATGACCAACC CCTGCTTGGA CAAGATGACC 
ATCCCGAGGG CCAAGCTGAG CGCGGGCAAC AACTGGACAT CGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008319987.1
CDS	284..3091
Translation	MFSKCPVLLLLNVLFLVRALSAHECTPCHLRTCPLKAAHGCEGGRTPVKDPCGCCDQCARLEWEPCGGQDWTHGYCALGLTCASFNSTEAATIPEIGVCKVLPDYPEAGLEDERCPLVTGCDTAGGQCVCDARHSCLGSFTYPDQETCMKSFKSEGRRHEHRERHREKYVGPSNPACMFSGCNLTTEGCVCESQSCHHHFAYINRSQCQEAAEELRCARVTCPAVQVPSCPHGSVLTKSYTPPAGCCPSIHPQCTCDFHACPKPQCPGGQKAVRLTRASGQPGDCCDVFECQKVSPKCVHNEKEFSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSGNECPYGFEQDTNGCLLCQCLGNDSCPELGKYCSLQCPMGYERDDFGCEVCECSIPTPRCRPLTCSKTCPYGYARNKHGCEMCRCVKCPPFTCDKHCINGYQQNKKGCSVCMCKEGSPIPRTTPTLPVPKYCLTSNGHRYEEDEGWHDGCRDCYCHSGREMCVLITCPVPNCPQPVVRPDQCCPTCEDESGSGQPDGMDMVVCRAPGGEFYVEGETWNLDECTSCTCRGGRVLCDTEVCPPALCQTPTRNKDTCCHVCPEESPLLPVNNNEQEYCITNDGDVLLNGDSWKANACTSCTCNNGTIQCFSQHCPAANCRVPVLRKGQCCPYCIDMTTLVPVTVSTSGRAATVKSAVLTTTVSPLPPIIADTDEPGLGENYPSHTEMTLIYQSAAWILAGLLLAIIIFLIVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKNDCGIIHFSPDMTNPCLDKMTIPRAKLSAGNNWTSY

Target ORF information:

RefSeq Version	XM_008321765.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008321765.2

ORF Insert Sequence:

ATGTTCAGCA AGTGTCCTGT CCTCCTCCTC CTGAACGTCC TGTTTCTGGT CCGCGCTCTC 
TCCGCTCATG AGTGCACGCC GTGTCATCTG CGGACTTGTC CGCTGAAAGC CGCTCACGGC 
TGCGAAGGTG GTCGGACTCC GGTGAAGGAT CCGTGCGGAT GCTGCGACCA GTGCGCCCGC 
CTGGAGTGGG AGCCCTGCGG CGGACAGGAC TGGACGCACG GCTACTGCGC CCTCGGGCTA 
ACGTGTGCCT CCTTCAACAG CACCGAGGCT GCCACAATCC CGGAGATCGG AGTATGTAAA 
GTACTCCCTG ACTACCCAGA GGCTGGCCTC GAAGACGAGC GCTGCCCCCT AGTAACGGGC 
TGTGACACAG CAGGAGGACA GTGCGTGTGT GACGCCCGTC ACTCCTGTCT GGGCTCCTTC 
ACTTACCCCG ACCAAGAAAC CTGTATGAAG TCATTCAAGT CAGAGGGTCG GAGGCACGAG 
CACAGGGAGA GACACAGAGA GAAGTATGTG GGACCGTCCA ACCCGGCCTG CATGTTCTCG 
GGCTGTAACC TGACCACCGA GGGCTGTGTG TGCGAGTCTC AGAGCTGCCA TCACCACTTT 
GCCTACATCA ACCGCAGCCA GTGCCAGGAA GCCGCAGAGG AGCTGAGGTG TGCCCGGGTG 
ACATGCCCTG CTGTGCAGGT GCCCTCCTGC CCCCATGGTT CAGTGCTAAC CAAGAGTTAC 
ACGCCCCCTG CTGGCTGCTG TCCATCCATA CATCCCCAGT GCACATGCGA CTTCCACGCT 
TGCCCTAAGC CCCAGTGCCC CGGCGGGCAG AAAGCCGTGC GGCTCACTCG GGCCAGTGGT 
CAGCCGGGAG ACTGCTGTGA CGTCTTCGAG TGCCAAAAAG TCTCTCCCAA GTGCGTCCAC 
AACGAGAAGG AGTTCTCAGA GGGTGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGTCGAGGAG GAATCTCCTT CTGCTCCAAA GCCGAGTGTG CGGAGCTGGA CTGTGAAAAC 
TTCTACATTC CTGAGGGGGA GTGCTGCCCT GTCTGCATAG ATGTGGAGCT GCTGTCCATG 
GACAGCACAA AGGCCAGCTG CTGGGTGAAT AACAAGCTCC GGGCACATGA GGAGCAGTGG 
AAAGAGGATG ACTGCACCTT CTGCCAGTGT GTGGACGGGG ATCCACACTG CACGGCCATG 
GCCTGCAAAC AGAGCTGTCA AAACCCTGTC AAGATCCCCG GAGAATGCTG CCCCTTCTGT 
GAAGAGCCTT CGTACGAGAC AGTCAGCCCC TTGCTGTGTC CCCCTCTGGA GAACTGCTCC 
CTATCAGGGA ACGAGTGTCC CTATGGCTTT GAACAAGACA CAAACGGATG TCTCCTCTGT 
CAGTGTCTCG GCAATGACTC CTGCCCCGAA CTGGGAAAAT ACTGTTCCCT GCAATGCCCG 
ATGGGATACG AGAGGGACGA TTTTGGCTGT GAGGTGTGCG AGTGCAGCAT CCCCACGCCG 
AGGTGCCGAC CTCTGACCTG CAGTAAAACC TGCCCTTATG GATATGCGAG GAACAAGCAC 
GGCTGCGAAA TGTGCCGCTG TGTCAAGTGC CCTCCCTTCA CCTGCGACAA GCACTGCATC 
AACGGCTACC AGCAGAACAA GAAGGGTTGC TCCGTCTGCA TGTGCAAAGA GGGTAGCCCC 
ATTCCAAGGA CCACCCCCAC GCTGCCCGTA CCCAAGTACT GCCTCACCTC CAACGGTCAC 
AGATATGAGG AAGATGAAGG ATGGCATGAC GGATGTCGTG ACTGCTACTG TCACTCTGGA 
CGGGAGATGT GTGTTCTTAT TACCTGTCCT GTGCCCAACT GTCCTCAACC AGTGGTCAGG 
CCTGACCAGT GCTGTCCCAC ATGCGAAGAT GAGTCAGGCA GTGGTCAACC AGATGGGATG 
GACATGGTGG TGTGCAGAGC ACCAGGGGGG GAGTTTTACG TGGAGGGGGA GACCTGGAAC 
TTGGACGAGT GCACCAGCTG CACCTGCCGG GGGGGTCGCG TCCTGTGCGA CACGGAGGTG 
TGTCCACCAG CTCTGTGTCA GACCCCCACC AGAAACAAGG ACACCTGCTG CCACGTATGC 
CCAGAGGAGA GCCCACTGCT ACCTGTCAAC AACAACGAGC AGGAGTACTG CATCACCAAT 
GACGGAGACG TGCTACTGAA CGGAGACTCC TGGAAGGCGA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGCTTCTCT CAGCACTGCC CAGCTGCCAA CTGCAGGGTG 
CCCGTCTTAC GCAAAGGCCA GTGCTGCCCA TACTGTATTG ATATGACAAC GTTGGTGCCA 
GTGACGGTGT CCACCAGTGG TCGTGCTGCT ACAGTGAAGA GTGCAGTGTT GACAACCACT 
GTGTCACCAC TGCCGCCCAT CATCGCAGAT ACAGATGAGC CAGGTTTGGG CGAGAACTAT 
CCCAGCCACA CAGAGATGAC GCTCATCTAC CAATCAGCTG CCTGGATCCT GGCTGGTCTG 
CTTCTGGCCA TCATCATCTT CCTCATTGTA GCCTTGCTCA TCAACAAGAA AAAGAAGTGG 
GTTCAGATGT CGTGCTACAG TGCACCAAAG AAGACGGTAA TCCTGAAAAA GCACGTAAAC 
AAGAACTCGG TGGTCTACAT GGAGCCGTCC AAGGAGAACA AATTCCAAAA CGTGAAGAAC 
GACTGTGGCA TCATTCACTT CTCTCCCGAC ATGACCAACC CCTGCTTGGA CAAGATGACC 
ATCCCGAGGG CCAAGCTGAG CGCGGGCAAC AACTGGACAT CGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_008321765.3 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008319987.1 Cynoglossus semilaevis cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Price & Availability↑

CloneID	OCy35238
Clone ID Related Accession (Same CDS sequence)	XM_008321765.2 , XM_008321765.3
Accession Version	XM_008321765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024307.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_008321765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA AGTGTCCTGT CCTCCTCCTC CTGAACGTCC TGTTTCTGGT CCGCGCTCTC 
TCCGCTCATG AGTGCACGCC GTGTCATCTG CGGACTTGTC CGCTGAAAGC CGCTCACGGC 
TGCGAAGGTG GTCGGACTCC GGTGAAGGAT CCGTGCGGAT GCTGCGACCA GTGCGCCCGC 
CTGGAGTGGG AGCCCTGCGG CGGACAGGAC TGGACGCACG GCTACTGCGC CCTCGGGCTA 
ACGTGTGCCT CCTTCAACAG CACCGAGGCT GCCACAATCC CGGAGATCGG AGTATGTAAA 
GTACTCCCTG ACTACCCAGA GGCTGGCCTC GAAGACGAGC GCTGCCCCCT AGTAACGGGC 
TGTGACACAG CAGGAGGACA GTGCGTGTGT GACGCCCGTC ACTCCTGTCT GGGCTCCTTC 
ACTTACCCCG ACCAAGAAAC CTGTATGAAG TCATTCAAGT CAGAGGGTCG GAGGCACGAG 
CACAGGGAGA GACACAGAGA GAAGTATGTG GGACCGTCCA ACCCGGCCTG CATGTTCTCG 
GGCTGTAACC TGACCACCGA GGGCTGTGTG TGCGAGTCTC AGAGCTGCCA TCACCACTTT 
GCCTACATCA ACCGCAGCCA GTGCCAGGAA GCCGCAGAGG AGCTGAGGTG TGCCCGGGTG 
ACATGCCCTG CTGTGCAGGT GCCCTCCTGC CCCCATGGTT CAGTGCTAAC CAAGAGTTAC 
ACGCCCCCTG CTGGCTGCTG TCCATCCATA CATCCCCAGT GCACATGCGA CTTCCACGCT 
TGCCCTAAGC CCCAGTGCCC CGGCGGGCAG AAAGCCGTGC GGCTCACTCG GGCCAGTGGT 
CAGCCGGGAG ACTGCTGTGA CGTCTTCGAG TGCCAAAAAG TCTCTCCCAA GTGCGTCCAC 
AACGAGAAGG AGTTCTCAGA GGGTGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGTCGAGGAG GAATCTCCTT CTGCTCCAAA GCCGAGTGTG CGGAGCTGGA CTGTGAAAAC 
TTCTACATTC CTGAGGGGGA GTGCTGCCCT GTCTGCATAG ATGTGGAGCT GCTGTCCATG 
GACAGCACAA AGGCCAGCTG CTGGGTGAAT AACAAGCTCC GGGCACATGA GGAGCAGTGG 
AAAGAGGATG ACTGCACCTT CTGCCAGTGT GTGGACGGGG ATCCACACTG CACGGCCATG 
GCCTGCAAAC AGAGCTGTCA AAACCCTGTC AAGATCCCCG GAGAATGCTG CCCCTTCTGT 
GAAGAGCCTT CGTACGAGAC AGTCAGCCCC TTGCTGTGTC CCCCTCTGGA GAACTGCTCC 
CTATCAGGGA ACGAGTGTCC CTATGGCTTT GAACAAGACA CAAACGGATG TCTCCTCTGT 
CAGTGTCTCG GCAATGACTC CTGCCCCGAA CTGGGAAAAT ACTGTTCCCT GCAATGCCCG 
ATGGGATACG AGAGGGACGA TTTTGGCTGT GAGGTGTGCG AGTGCAGCAT CCCCACGCCG 
AGGTGCCGAC CTCTGACCTG CAGTAAAACC TGCCCTTATG GATATGCGAG GAACAAGCAC 
GGCTGCGAAA TGTGCCGCTG TGTCAAGTGC CCTCCCTTCA CCTGCGACAA GCACTGCATC 
AACGGCTACC AGCAGAACAA GAAGGGTTGC TCCGTCTGCA TGTGCAAAGA GGGTAGCCCC 
ATTCCAAGGA CCACCCCCAC GCTGCCCGTA CCCAAGTACT GCCTCACCTC CAACGGTCAC 
AGATATGAGG AAGATGAAGG ATGGCATGAC GGATGTCGTG ACTGCTACTG TCACTCTGGA 
CGGGAGATGT GTGTTCTTAT TACCTGTCCT GTGCCCAACT GTCCTCAACC AGTGGTCAGG 
CCTGACCAGT GCTGTCCCAC ATGCGAAGAT GAGTCAGGCA GTGGTCAACC AGATGGGATG 
GACATGGTGG TGTGCAGAGC ACCAGGGGGG GAGTTTTACG TGGAGGGGGA GACCTGGAAC 
TTGGACGAGT GCACCAGCTG CACCTGCCGG GGGGGTCGCG TCCTGTGCGA CACGGAGGTG 
TGTCCACCAG CTCTGTGTCA GACCCCCACC AGAAACAAGG ACACCTGCTG CCACGTATGC 
CCAGAGGAGA GCCCACTGCT ACCTGTCAAC AACAACGAGC AGGAGTACTG CATCACCAAT 
GACGGAGACG TGCTACTGAA CGGAGACTCC TGGAAGGCGA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGCTTCTCT CAGCACTGCC CAGCTGCCAA CTGCAGGGTG 
CCCGTCTTAC GCAAAGGCCA GTGCTGCCCA TACTGTATTG ATATGACAAC GTTGGTGCCA 
GTGACGGTGT CCACCAGTGG TCGTGCTGCT ACAGTGAAGA GTGCAGTGTT GACAACCACT 
GTGTCACCAC TGCCGCCCAT CATCGCAGAT ACAGATGAGC CAGGTTTGGG CGAGAACTAT 
CCCAGCCACA CAGAGATGAC GCTCATCTAC CAATCAGCTG CCTGGATCCT GGCTGGTCTG 
CTTCTGGCCA TCATCATCTT CCTCATTGTA GCCTTGCTCA TCAACAAGAA AAAGAAGTGG 
GTTCAGATGT CGTGCTACAG TGCACCAAAG AAGACGGTAA TCCTGAAAAA GCACGTAAAC 
AAGAACTCGG TGGTCTACAT GGAGCCGTCC AAGGAGAACA AATTCCAAAA CGTGAAGAAC 
GACTGTGGCA TCATTCACTT CTCTCCCGAC ATGACCAACC CCTGCTTGGA CAAGATGACC 
ATCCCGAGGG CCAAGCTGAG CGCGGGCAAC AACTGGACAT CGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008319987.1
CDS	591..3398
Translation	MFSKCPVLLLLNVLFLVRALSAHECTPCHLRTCPLKAAHGCEGGRTPVKDPCGCCDQCARLEWEPCGGQDWTHGYCALGLTCASFNSTEAATIPEIGVCKVLPDYPEAGLEDERCPLVTGCDTAGGQCVCDARHSCLGSFTYPDQETCMKSFKSEGRRHEHRERHREKYVGPSNPACMFSGCNLTTEGCVCESQSCHHHFAYINRSQCQEAAEELRCARVTCPAVQVPSCPHGSVLTKSYTPPAGCCPSIHPQCTCDFHACPKPQCPGGQKAVRLTRASGQPGDCCDVFECQKVSPKCVHNEKEFSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSGNECPYGFEQDTNGCLLCQCLGNDSCPELGKYCSLQCPMGYERDDFGCEVCECSIPTPRCRPLTCSKTCPYGYARNKHGCEMCRCVKCPPFTCDKHCINGYQQNKKGCSVCMCKEGSPIPRTTPTLPVPKYCLTSNGHRYEEDEGWHDGCRDCYCHSGREMCVLITCPVPNCPQPVVRPDQCCPTCEDESGSGQPDGMDMVVCRAPGGEFYVEGETWNLDECTSCTCRGGRVLCDTEVCPPALCQTPTRNKDTCCHVCPEESPLLPVNNNEQEYCITNDGDVLLNGDSWKANACTSCTCNNGTIQCFSQHCPAANCRVPVLRKGQCCPYCIDMTTLVPVTVSTSGRAATVKSAVLTTTVSPLPPIIADTDEPGLGENYPSHTEMTLIYQSAAWILAGLLLAIIIFLIVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKNDCGIIHFSPDMTNPCLDKMTIPRAKLSAGNNWTSY

Target ORF information:

RefSeq Version	XM_008321765.3
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008321765.3

ORF Insert Sequence:

ATGTTCAGCA AGTGTCCTGT CCTCCTCCTC CTGAACGTCC TGTTTCTGGT CCGCGCTCTC 
TCCGCTCATG AGTGCACGCC GTGTCATCTG CGGACTTGTC CGCTGAAAGC CGCTCACGGC 
TGCGAAGGTG GTCGGACTCC GGTGAAGGAT CCGTGCGGAT GCTGCGACCA GTGCGCCCGC 
CTGGAGTGGG AGCCCTGCGG CGGACAGGAC TGGACGCACG GCTACTGCGC CCTCGGGCTA 
ACGTGTGCCT CCTTCAACAG CACCGAGGCT GCCACAATCC CGGAGATCGG AGTATGTAAA 
GTACTCCCTG ACTACCCAGA GGCTGGCCTC GAAGACGAGC GCTGCCCCCT AGTAACGGGC 
TGTGACACAG CAGGAGGACA GTGCGTGTGT GACGCCCGTC ACTCCTGTCT GGGCTCCTTC 
ACTTACCCCG ACCAAGAAAC CTGTATGAAG TCATTCAAGT CAGAGGGTCG GAGGCACGAG 
CACAGGGAGA GACACAGAGA GAAGTATGTG GGACCGTCCA ACCCGGCCTG CATGTTCTCG 
GGCTGTAACC TGACCACCGA GGGCTGTGTG TGCGAGTCTC AGAGCTGCCA TCACCACTTT 
GCCTACATCA ACCGCAGCCA GTGCCAGGAA GCCGCAGAGG AGCTGAGGTG TGCCCGGGTG 
ACATGCCCTG CTGTGCAGGT GCCCTCCTGC CCCCATGGTT CAGTGCTAAC CAAGAGTTAC 
ACGCCCCCTG CTGGCTGCTG TCCATCCATA CATCCCCAGT GCACATGCGA CTTCCACGCT 
TGCCCTAAGC CCCAGTGCCC CGGCGGGCAG AAAGCCGTGC GGCTCACTCG GGCCAGTGGT 
CAGCCGGGAG ACTGCTGTGA CGTCTTCGAG TGCCAAAAAG TCTCTCCCAA GTGCGTCCAC 
AACGAGAAGG AGTTCTCAGA GGGTGAGGTG TACCGCATGG ACCCCTGCTG GCTGTGTCAG 
TGTCGAGGAG GAATCTCCTT CTGCTCCAAA GCCGAGTGTG CGGAGCTGGA CTGTGAAAAC 
TTCTACATTC CTGAGGGGGA GTGCTGCCCT GTCTGCATAG ATGTGGAGCT GCTGTCCATG 
GACAGCACAA AGGCCAGCTG CTGGGTGAAT AACAAGCTCC GGGCACATGA GGAGCAGTGG 
AAAGAGGATG ACTGCACCTT CTGCCAGTGT GTGGACGGGG ATCCACACTG CACGGCCATG 
GCCTGCAAAC AGAGCTGTCA AAACCCTGTC AAGATCCCCG GAGAATGCTG CCCCTTCTGT 
GAAGAGCCTT CGTACGAGAC AGTCAGCCCC TTGCTGTGTC CCCCTCTGGA GAACTGCTCC 
CTATCAGGGA ACGAGTGTCC CTATGGCTTT GAACAAGACA CAAACGGATG TCTCCTCTGT 
CAGTGTCTCG GCAATGACTC CTGCCCCGAA CTGGGAAAAT ACTGTTCCCT GCAATGCCCG 
ATGGGATACG AGAGGGACGA TTTTGGCTGT GAGGTGTGCG AGTGCAGCAT CCCCACGCCG 
AGGTGCCGAC CTCTGACCTG CAGTAAAACC TGCCCTTATG GATATGCGAG GAACAAGCAC 
GGCTGCGAAA TGTGCCGCTG TGTCAAGTGC CCTCCCTTCA CCTGCGACAA GCACTGCATC 
AACGGCTACC AGCAGAACAA GAAGGGTTGC TCCGTCTGCA TGTGCAAAGA GGGTAGCCCC 
ATTCCAAGGA CCACCCCCAC GCTGCCCGTA CCCAAGTACT GCCTCACCTC CAACGGTCAC 
AGATATGAGG AAGATGAAGG ATGGCATGAC GGATGTCGTG ACTGCTACTG TCACTCTGGA 
CGGGAGATGT GTGTTCTTAT TACCTGTCCT GTGCCCAACT GTCCTCAACC AGTGGTCAGG 
CCTGACCAGT GCTGTCCCAC ATGCGAAGAT GAGTCAGGCA GTGGTCAACC AGATGGGATG 
GACATGGTGG TGTGCAGAGC ACCAGGGGGG GAGTTTTACG TGGAGGGGGA GACCTGGAAC 
TTGGACGAGT GCACCAGCTG CACCTGCCGG GGGGGTCGCG TCCTGTGCGA CACGGAGGTG 
TGTCCACCAG CTCTGTGTCA GACCCCCACC AGAAACAAGG ACACCTGCTG CCACGTATGC 
CCAGAGGAGA GCCCACTGCT ACCTGTCAAC AACAACGAGC AGGAGTACTG CATCACCAAT 
GACGGAGACG TGCTACTGAA CGGAGACTCC TGGAAGGCGA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGCTTCTCT CAGCACTGCC CAGCTGCCAA CTGCAGGGTG 
CCCGTCTTAC GCAAAGGCCA GTGCTGCCCA TACTGTATTG ATATGACAAC GTTGGTGCCA 
GTGACGGTGT CCACCAGTGG TCGTGCTGCT ACAGTGAAGA GTGCAGTGTT GACAACCACT 
GTGTCACCAC TGCCGCCCAT CATCGCAGAT ACAGATGAGC CAGGTTTGGG CGAGAACTAT 
CCCAGCCACA CAGAGATGAC GCTCATCTAC CAATCAGCTG CCTGGATCCT GGCTGGTCTG 
CTTCTGGCCA TCATCATCTT CCTCATTGTA GCCTTGCTCA TCAACAAGAA AAAGAAGTGG 
GTTCAGATGT CGTGCTACAG TGCACCAAAG AAGACGGTAA TCCTGAAAAA GCACGTAAAC 
AAGAACTCGG TGGTCTACAT GGAGCCGTCC AAGGAGAACA AATTCCAAAA CGTGAAGAAC 
GACTGTGGCA TCATTCACTT CTCTCCCGAC ATGACCAACC CCTGCTTGGA CAAGATGACC 
ATCCCGAGGG CCAAGCTGAG CGCGGGCAAC AACTGGACAT CGTACTAG