LOC103384079 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103384079
Entrez Gene ID	103384079
Full Name	uncharacterized LOC103384079
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008317449.3	XP_008315671.1	uncharacterized protein LOC103384079
XM_008317449.2	XP_008315671.1	uncharacterized protein LOC103384079

Pathways from BioSystems
Gene Ontology

The following LOC103384079 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103384079 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy60398	XM_008317449.3 Latest version!	Cynoglossus semilaevis uncharacterized LOC103384079 (LOC103384079), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OCy60398	XM_008317449.2	Cynoglossus semilaevis uncharacterized LOC103384079 (LOC103384079), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103384079 ( XM_008317449.3 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008315671.1 Cynoglossus semilaevis uncharacterized LOC103384079 (LOC103384079), mRNA.

Price & Availability↑

CloneID	OCy60398
Clone ID Related Accession (Same CDS sequence)	XM_008317449.3 , XM_008317449.2
Accession Version	XM_008317449.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein LOC103384079
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024315.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_008317449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTCC TGACTGCCAC CCTCGTGTTT CCTTTAATTG GTTCGATCTA CGGCACTCAG 
TTTAAAGCAA CTCTAAACAT GGCAGGAGTC ACCGGACAGG TGCAGTTTGA CTCCACATCC 
CAGACTGCCG CTGTTGCAGT TTCTGGTGCT GGATCCTGTG GTCTAGTGGA ACTGTTCCTC 
AGTGAGTTCC CCGTCATGTA TGGCCATTTT CCTGAACCTT GTTCGGAAGC AAATATCGGT 
CCCAAGGTTT TTTCCTTCAC AGCAGATCCC GCCTCATCGT CCACCGTCAA TGTGTCACAC 
CTCTTTGAAC GAAGACCAAA CCTGGACGAC TTTACTTTGT CCTTGAAGAC GTGTGACGAG 
ATCATGGTTT GTACAGTGGT AGGTCAAGGC AGATCATCCT TGACTTTTCA GGCTAGATTC 
ACTGGTCCAA TTGCCGGTAA TGTTTACATT CGAACAAACT CTGAACAAAG TAACGTCAGG 
CTTCTAGCAG ACCTGGTCAC AATCGGTCAA GTAAATGCCA CTAACACCAG TATCACTCTT 
CTGGGATCTA CAAGCACCGC TGCAAACTGT GATGTTCTGC TTGGAAGCTT GAAGGCTAGC 
ACTTTGTCAA GTTTGGGAAA GGTCAAAGTC GGAACACCTC TGGATCTTAA GAAATCCCGT 
CTGGACAACT GGGAAAACTG GAGTCTTCGA TTTCTTCTTT ACCACGCGGG CTCAAGTTAC 
AAGTGTGCTC GCGTTTATAA TGTCCCTGGT AAACAGGTGA CTGCTGTTAT TAATATGAAG 
GGGATTAAAG GGTATTTTAC CTTTAAACAA GCTTCATGTT TTGATGCAAC AGAGATGAGA 
GTTAACCTCA CCAACCTGCA GGGCAGAGTC GGTCCATATC ACGTCCACCT CTATCCGGTC 
CCTTCAGTCA GAACAAGCAA CAAAGAAAGT TTATGCTCCA ATGAAAATGT TGGTGGACAT 
TGGAATCCCT TTAAAATCAA GACTGCGGAC CCGACATATC CTAAAGGTCC TGGGTCAACA 
CATGACAAGT ACGAGGTTGG AGATCTGAGT ACGAGGCACG TGTCCCTCAC AGATATGAAT 
GACACTGACG TAATGTTTAC GGATTTCAAC CTTCCTCTGT TTGGACAAAA CAGTATTGTC 
GGTCGCTCAG TTGTTATACA CCAAACAGAC GGTTCCAGGT ATGTTTGCTC TAGCATCAGT 
TATCCTGGCA AAGTAGTTGT CGGCAGAGCC AGGTTTCAGA GTCATGTGGT TGGTGAGATC 
TGGTTCACCC AACTGGAAAA CAACCCTTTG TCCGATGTAT CCATCTTCAT GGATCTGGCT 
TATGGAAGTC CGACAACCAG CCCCACCATT AACCACCACT GGCATATTCA CCACTACCCT 
ATAAGCTCAG AGAGAGATGA TGACGAGAGG CGCTGCAGCA CAACAGAAGC CCACTGGAAC 
CCCTTCCACA TCGACACAGA AGACAGCAGC TATTTGCTAC ACTGTGGCCC AGCCAGACCT 
CTCTCCTGTG AGAGTGGAGA CCTGTCCAGC AAGCACAGAA CCATCGACCT CACCACCCAA 
GTTGGTTCAG TGCACGCCAA AAGCTTCTTC ACCGATGTCA CTTCCTGGTT GCAGGATGCA 
GGCGTTATTG GTCGTTCTGT GGTCATTCAT CAAGCCAACA GAGGAGGACC AAGAATCGCT 
TGTGCAAATG TCACCATGGT GCGAGAGCAC AAAGCCAGAC TGGGTAACTG GTTTGGTCCT 
GGACTGTCCG GAGGTCAAAT GCGGCTCGTT CAAGGTGCCG TGCAAGGACC CACGACTATC 
AACGTGTCTT TGATGAACCT CGACTCTCTG GCGGGGGGTT ACCACGTTCA CCTGCTGCCC 
ATCAAACCTG GCAGCGTGGA GCCCTGCTCT GATGCAAACA TCATGGGTCA TTTCAACCCA 
TTAGGCTGGA ACATATCGGA TTCTCCCGCA CCAGGAACTG GCACAATGGA CCAGTATGAA 
ATCGGAGACA TCAGTGGGAA GTTTGGAATG CTGAATGGTC TCAGCCTACT TGAAGCTCTG 
CACTTGGATC CTGATATTCA ATTAACTGGA CCCCACAGCA TTGCGGGAAG GTCCCTGGTG 
GTTCACTATG CCAATGGCTC GAGAATGAGG TGTGCTGACA TTTTAGCTGA AAGACATACT 
GAAGGACAAT GGATTGTTGC CAAGGCATTA TTCAACAGCA CAGTTACTGG GGCAGTACAA 
CTGCGTCAGC AGGTGTTTCC CGACGGGAGT AGTAATGATG TCACACTTCT GGTGACGCTG 
AAATCATCTG AACGGAATAC TCCTTCACTG TCACTGTTTA TCTTCAATGG CCAATGCAGC 
AACTTGAGAG ATGTTCATAA TCCCTTCAAC ATGACATCAG GAGACTCCAG GTGTTCATTA 
GTTCACCCTC TGAGCTGTGT GCTAGGGGAA ATATCCACCA GGCACGGTCC AGTCAGTCTA 
ACCCACGGTC AGCTGTTCAC TGACAGTATC ATCCAGCTGT CTGGAGACAA CACTGTGGTC 
CACAAATGTT TGGTGCTGAA AAATGGAGAT AGCGTCACAG CGTGTGCCAA CATTCTGCCA 
GAATCCCCAA CGGCAGAGCA GAAATTTCCT ACTCGGACAG ATTTCAGCAG ATACGATTTC 
CGTAGAAGAG TTGCAAGTGT CCTGCAGATG GACGTTGCGA GAGTCACCAT CTTGCCCGAC 
TCTCCGTCCT CTGTAGCAGA TGGAAGGTGT CAAGAGGTCA ACTTCATGAT ATGTGGAACT 
GTCAGCCCCG AACTCCTGGA GTCGGTGAAA ACCAGCGAGA AGATGGGTAT TTACAGAGAG 
TCTGACAAGT GTGCAACAAG TGCAGTTCTC CGTGCGCTGC CAGAAGTTTT TCTATTCGGC 
CTGATGTTTG CTGTCGCCTG CCTGCTGCCG TCTTCAGTTT ATTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008315671.1
CDS	279..3206
Translation	MLLLTATLVFPLIGSIYGTQFKATLNMAGVTGQVQFDSTSQTAAVAVSGAGSCGLVELFLSEFPVMYGHFPEPCSEANIGPKVFSFTADPASSSTVNVSHLFERRPNLDDFTLSLKTCDEIMVCTVVGQGRSSLTFQARFTGPIAGNVYIRTNSEQSNVRLLADLVTIGQVNATNTSITLLGSTSTAANCDVLLGSLKASTLSSLGKVKVGTPLDLKKSRLDNWENWSLRFLLYHAGSSYKCARVYNVPGKQVTAVINMKGIKGYFTFKQASCFDATEMRVNLTNLQGRVGPYHVHLYPVPSVRTSNKESLCSNENVGGHWNPFKIKTADPTYPKGPGSTHDKYEVGDLSTRHVSLTDMNDTDVMFTDFNLPLFGQNSIVGRSVVIHQTDGSRYVCSSISYPGKVVVGRARFQSHVVGEIWFTQLENNPLSDVSIFMDLAYGSPTTSPTINHHWHIHHYPISSERDDDERRCSTTEAHWNPFHIDTEDSSYLLHCGPARPLSCESGDLSSKHRTIDLTTQVGSVHAKSFFTDVTSWLQDAGVIGRSVVIHQANRGGPRIACANVTMVREHKARLGNWFGPGLSGGQMRLVQGAVQGPTTINVSLMNLDSLAGGYHVHLLPIKPGSVEPCSDANIMGHFNPLGWNISDSPAPGTGTMDQYEIGDISGKFGMLNGLSLLEALHLDPDIQLTGPHSIAGRSLVVHYANGSRMRCADILAERHTEGQWIVAKALFNSTVTGAVQLRQQVFPDGSSNDVTLLVTLKSSERNTPSLSLFIFNGQCSNLRDVHNPFNMTSGDSRCSLVHPLSCVLGEISTRHGPVSLTHGQLFTDSIIQLSGDNTVVHKCLVLKNGDSVTACANILPESPTAEQKFPTRTDFSRYDFRRRVASVLQMDVARVTILPDSPSSVADGRCQEVNFMICGTVSPELLESVKTSEKMGIYRESDKCATSAVLRALPEVFLFGLMFAVACLLPSSVYS

Target ORF information:

RefSeq Version	XM_008317449.3
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis uncharacterized LOC103384079 (LOC103384079), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008317449.3

ORF Insert Sequence:

ATGCTCCTCC TGACTGCCAC CCTCGTGTTT CCTTTAATTG GTTCGATCTA CGGCACTCAG 
TTTAAAGCAA CTCTAAACAT GGCAGGAGTC ACCGGACAGG TGCAGTTTGA CTCCACATCC 
CAGACTGCCG CTGTTGCAGT TTCTGGTGCT GGATCCTGTG GTCTAGTGGA ACTGTTCCTC 
AGTGAGTTCC CCGTCATGTA TGGCCATTTT CCTGAACCTT GTTCGGAAGC AAATATCGGT 
CCCAAGGTTT TTTCCTTCAC AGCAGATCCC GCCTCATCGT CCACCGTCAA TGTGTCACAC 
CTCTTTGAAC GAAGACCAAA CCTGGACGAC TTTACTTTGT CCTTGAAGAC GTGTGACGAG 
ATCATGGTTT GTACAGTGGT AGGTCAAGGC AGATCATCCT TGACTTTTCA GGCTAGATTC 
ACTGGTCCAA TTGCCGGTAA TGTTTACATT CGAACAAACT CTGAACAAAG TAACGTCAGG 
CTTCTAGCAG ACCTGGTCAC AATCGGTCAA GTAAATGCCA CTAACACCAG TATCACTCTT 
CTGGGATCTA CAAGCACCGC TGCAAACTGT GATGTTCTGC TTGGAAGCTT GAAGGCTAGC 
ACTTTGTCAA GTTTGGGAAA GGTCAAAGTC GGAACACCTC TGGATCTTAA GAAATCCCGT 
CTGGACAACT GGGAAAACTG GAGTCTTCGA TTTCTTCTTT ACCACGCGGG CTCAAGTTAC 
AAGTGTGCTC GCGTTTATAA TGTCCCTGGT AAACAGGTGA CTGCTGTTAT TAATATGAAG 
GGGATTAAAG GGTATTTTAC CTTTAAACAA GCTTCATGTT TTGATGCAAC AGAGATGAGA 
GTTAACCTCA CCAACCTGCA GGGCAGAGTC GGTCCATATC ACGTCCACCT CTATCCGGTC 
CCTTCAGTCA GAACAAGCAA CAAAGAAAGT TTATGCTCCA ATGAAAATGT TGGTGGACAT 
TGGAATCCCT TTAAAATCAA GACTGCGGAC CCGACATATC CTAAAGGTCC TGGGTCAACA 
CATGACAAGT ACGAGGTTGG AGATCTGAGT ACGAGGCACG TGTCCCTCAC AGATATGAAT 
GACACTGACG TAATGTTTAC GGATTTCAAC CTTCCTCTGT TTGGACAAAA CAGTATTGTC 
GGTCGCTCAG TTGTTATACA CCAAACAGAC GGTTCCAGGT ATGTTTGCTC TAGCATCAGT 
TATCCTGGCA AAGTAGTTGT CGGCAGAGCC AGGTTTCAGA GTCATGTGGT TGGTGAGATC 
TGGTTCACCC AACTGGAAAA CAACCCTTTG TCCGATGTAT CCATCTTCAT GGATCTGGCT 
TATGGAAGTC CGACAACCAG CCCCACCATT AACCACCACT GGCATATTCA CCACTACCCT 
ATAAGCTCAG AGAGAGATGA TGACGAGAGG CGCTGCAGCA CAACAGAAGC CCACTGGAAC 
CCCTTCCACA TCGACACAGA AGACAGCAGC TATTTGCTAC ACTGTGGCCC AGCCAGACCT 
CTCTCCTGTG AGAGTGGAGA CCTGTCCAGC AAGCACAGAA CCATCGACCT CACCACCCAA 
GTTGGTTCAG TGCACGCCAA AAGCTTCTTC ACCGATGTCA CTTCCTGGTT GCAGGATGCA 
GGCGTTATTG GTCGTTCTGT GGTCATTCAT CAAGCCAACA GAGGAGGACC AAGAATCGCT 
TGTGCAAATG TCACCATGGT GCGAGAGCAC AAAGCCAGAC TGGGTAACTG GTTTGGTCCT 
GGACTGTCCG GAGGTCAAAT GCGGCTCGTT CAAGGTGCCG TGCAAGGACC CACGACTATC 
AACGTGTCTT TGATGAACCT CGACTCTCTG GCGGGGGGTT ACCACGTTCA CCTGCTGCCC 
ATCAAACCTG GCAGCGTGGA GCCCTGCTCT GATGCAAACA TCATGGGTCA TTTCAACCCA 
TTAGGCTGGA ACATATCGGA TTCTCCCGCA CCAGGAACTG GCACAATGGA CCAGTATGAA 
ATCGGAGACA TCAGTGGGAA GTTTGGAATG CTGAATGGTC TCAGCCTACT TGAAGCTCTG 
CACTTGGATC CTGATATTCA ATTAACTGGA CCCCACAGCA TTGCGGGAAG GTCCCTGGTG 
GTTCACTATG CCAATGGCTC GAGAATGAGG TGTGCTGACA TTTTAGCTGA AAGACATACT 
GAAGGACAAT GGATTGTTGC CAAGGCATTA TTCAACAGCA CAGTTACTGG GGCAGTACAA 
CTGCGTCAGC AGGTGTTTCC CGACGGGAGT AGTAATGATG TCACACTTCT GGTGACGCTG 
AAATCATCTG AACGGAATAC TCCTTCACTG TCACTGTTTA TCTTCAATGG CCAATGCAGC 
AACTTGAGAG ATGTTCATAA TCCCTTCAAC ATGACATCAG GAGACTCCAG GTGTTCATTA 
GTTCACCCTC TGAGCTGTGT GCTAGGGGAA ATATCCACCA GGCACGGTCC AGTCAGTCTA 
ACCCACGGTC AGCTGTTCAC TGACAGTATC ATCCAGCTGT CTGGAGACAA CACTGTGGTC 
CACAAATGTT TGGTGCTGAA AAATGGAGAT AGCGTCACAG CGTGTGCCAA CATTCTGCCA 
GAATCCCCAA CGGCAGAGCA GAAATTTCCT ACTCGGACAG ATTTCAGCAG ATACGATTTC 
CGTAGAAGAG TTGCAAGTGT CCTGCAGATG GACGTTGCGA GAGTCACCAT CTTGCCCGAC 
TCTCCGTCCT CTGTAGCAGA TGGAAGGTGT CAAGAGGTCA ACTTCATGAT ATGTGGAACT 
GTCAGCCCCG AACTCCTGGA GTCGGTGAAA ACCAGCGAGA AGATGGGTAT TTACAGAGAG 
TCTGACAAGT GTGCAACAAG TGCAGTTCTC CGTGCGCTGC CAGAAGTTTT TCTATTCGGC 
CTGATGTTTG CTGTCGCCTG CCTGCTGCCG TCTTCAGTTT ATTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103384079 ( XM_008317449.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008315671.1 Cynoglossus semilaevis uncharacterized LOC103384079 (LOC103384079), mRNA.

Price & Availability↑

CloneID	OCy60398
Clone ID Related Accession (Same CDS sequence)	XM_008317449.3 , XM_008317449.2
Accession Version	XM_008317449.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein LOC103384079
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024315.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008317449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTCC TGACTGCCAC CCTCGTGTTT CCTTTAATTG GTTCGATCTA CGGCACTCAG 
TTTAAAGCAA CTCTAAACAT GGCAGGAGTC ACCGGACAGG TGCAGTTTGA CTCCACATCC 
CAGACTGCCG CTGTTGCAGT TTCTGGTGCT GGATCCTGTG GTCTAGTGGA ACTGTTCCTC 
AGTGAGTTCC CCGTCATGTA TGGCCATTTT CCTGAACCTT GTTCGGAAGC AAATATCGGT 
CCCAAGGTTT TTTCCTTCAC AGCAGATCCC GCCTCATCGT CCACCGTCAA TGTGTCACAC 
CTCTTTGAAC GAAGACCAAA CCTGGACGAC TTTACTTTGT CCTTGAAGAC GTGTGACGAG 
ATCATGGTTT GTACAGTGGT AGGTCAAGGC AGATCATCCT TGACTTTTCA GGCTAGATTC 
ACTGGTCCAA TTGCCGGTAA TGTTTACATT CGAACAAACT CTGAACAAAG TAACGTCAGG 
CTTCTAGCAG ACCTGGTCAC AATCGGTCAA GTAAATGCCA CTAACACCAG TATCACTCTT 
CTGGGATCTA CAAGCACCGC TGCAAACTGT GATGTTCTGC TTGGAAGCTT GAAGGCTAGC 
ACTTTGTCAA GTTTGGGAAA GGTCAAAGTC GGAACACCTC TGGATCTTAA GAAATCCCGT 
CTGGACAACT GGGAAAACTG GAGTCTTCGA TTTCTTCTTT ACCACGCGGG CTCAAGTTAC 
AAGTGTGCTC GCGTTTATAA TGTCCCTGGT AAACAGGTGA CTGCTGTTAT TAATATGAAG 
GGGATTAAAG GGTATTTTAC CTTTAAACAA GCTTCATGTT TTGATGCAAC AGAGATGAGA 
GTTAACCTCA CCAACCTGCA GGGCAGAGTC GGTCCATATC ACGTCCACCT CTATCCGGTC 
CCTTCAGTCA GAACAAGCAA CAAAGAAAGT TTATGCTCCA ATGAAAATGT TGGTGGACAT 
TGGAATCCCT TTAAAATCAA GACTGCGGAC CCGACATATC CTAAAGGTCC TGGGTCAACA 
CATGACAAGT ACGAGGTTGG AGATCTGAGT ACGAGGCACG TGTCCCTCAC AGATATGAAT 
GACACTGACG TAATGTTTAC GGATTTCAAC CTTCCTCTGT TTGGACAAAA CAGTATTGTC 
GGTCGCTCAG TTGTTATACA CCAAACAGAC GGTTCCAGGT ATGTTTGCTC TAGCATCAGT 
TATCCTGGCA AAGTAGTTGT CGGCAGAGCC AGGTTTCAGA GTCATGTGGT TGGTGAGATC 
TGGTTCACCC AACTGGAAAA CAACCCTTTG TCCGATGTAT CCATCTTCAT GGATCTGGCT 
TATGGAAGTC CGACAACCAG CCCCACCATT AACCACCACT GGCATATTCA CCACTACCCT 
ATAAGCTCAG AGAGAGATGA TGACGAGAGG CGCTGCAGCA CAACAGAAGC CCACTGGAAC 
CCCTTCCACA TCGACACAGA AGACAGCAGC TATTTGCTAC ACTGTGGCCC AGCCAGACCT 
CTCTCCTGTG AGAGTGGAGA CCTGTCCAGC AAGCACAGAA CCATCGACCT CACCACCCAA 
GTTGGTTCAG TGCACGCCAA AAGCTTCTTC ACCGATGTCA CTTCCTGGTT GCAGGATGCA 
GGCGTTATTG GTCGTTCTGT GGTCATTCAT CAAGCCAACA GAGGAGGACC AAGAATCGCT 
TGTGCAAATG TCACCATGGT GCGAGAGCAC AAAGCCAGAC TGGGTAACTG GTTTGGTCCT 
GGACTGTCCG GAGGTCAAAT GCGGCTCGTT CAAGGTGCCG TGCAAGGACC CACGACTATC 
AACGTGTCTT TGATGAACCT CGACTCTCTG GCGGGGGGTT ACCACGTTCA CCTGCTGCCC 
ATCAAACCTG GCAGCGTGGA GCCCTGCTCT GATGCAAACA TCATGGGTCA TTTCAACCCA 
TTAGGCTGGA ACATATCGGA TTCTCCCGCA CCAGGAACTG GCACAATGGA CCAGTATGAA 
ATCGGAGACA TCAGTGGGAA GTTTGGAATG CTGAATGGTC TCAGCCTACT TGAAGCTCTG 
CACTTGGATC CTGATATTCA ATTAACTGGA CCCCACAGCA TTGCGGGAAG GTCCCTGGTG 
GTTCACTATG CCAATGGCTC GAGAATGAGG TGTGCTGACA TTTTAGCTGA AAGACATACT 
GAAGGACAAT GGATTGTTGC CAAGGCATTA TTCAACAGCA CAGTTACTGG GGCAGTACAA 
CTGCGTCAGC AGGTGTTTCC CGACGGGAGT AGTAATGATG TCACACTTCT GGTGACGCTG 
AAATCATCTG AACGGAATAC TCCTTCACTG TCACTGTTTA TCTTCAATGG CCAATGCAGC 
AACTTGAGAG ATGTTCATAA TCCCTTCAAC ATGACATCAG GAGACTCCAG GTGTTCATTA 
GTTCACCCTC TGAGCTGTGT GCTAGGGGAA ATATCCACCA GGCACGGTCC AGTCAGTCTA 
ACCCACGGTC AGCTGTTCAC TGACAGTATC ATCCAGCTGT CTGGAGACAA CACTGTGGTC 
CACAAATGTT TGGTGCTGAA AAATGGAGAT AGCGTCACAG CGTGTGCCAA CATTCTGCCA 
GAATCCCCAA CGGCAGAGCA GAAATTTCCT ACTCGGACAG ATTTCAGCAG ATACGATTTC 
CGTAGAAGAG TTGCAAGTGT CCTGCAGATG GACGTTGCGA GAGTCACCAT CTTGCCCGAC 
TCTCCGTCCT CTGTAGCAGA TGGAAGGTGT CAAGAGGTCA ACTTCATGAT ATGTGGAACT 
GTCAGCCCCG AACTCCTGGA GTCGGTGAAA ACCAGCGAGA AGATGGGTAT TTACAGAGAG 
TCTGACAAGT GTGCAACAAG TGCAGTTCTC CGTGCGCTGC CAGAAGTTTT TCTATTCGGC 
CTGATGTTTG CTGTCGCCTG CCTGCTGCCG TCTTCAGTTT ATTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008315671.1
CDS	167..3094
Translation	MLLLTATLVFPLIGSIYGTQFKATLNMAGVTGQVQFDSTSQTAAVAVSGAGSCGLVELFLSEFPVMYGHFPEPCSEANIGPKVFSFTADPASSSTVNVSHLFERRPNLDDFTLSLKTCDEIMVCTVVGQGRSSLTFQARFTGPIAGNVYIRTNSEQSNVRLLADLVTIGQVNATNTSITLLGSTSTAANCDVLLGSLKASTLSSLGKVKVGTPLDLKKSRLDNWENWSLRFLLYHAGSSYKCARVYNVPGKQVTAVINMKGIKGYFTFKQASCFDATEMRVNLTNLQGRVGPYHVHLYPVPSVRTSNKESLCSNENVGGHWNPFKIKTADPTYPKGPGSTHDKYEVGDLSTRHVSLTDMNDTDVMFTDFNLPLFGQNSIVGRSVVIHQTDGSRYVCSSISYPGKVVVGRARFQSHVVGEIWFTQLENNPLSDVSIFMDLAYGSPTTSPTINHHWHIHHYPISSERDDDERRCSTTEAHWNPFHIDTEDSSYLLHCGPARPLSCESGDLSSKHRTIDLTTQVGSVHAKSFFTDVTSWLQDAGVIGRSVVIHQANRGGPRIACANVTMVREHKARLGNWFGPGLSGGQMRLVQGAVQGPTTINVSLMNLDSLAGGYHVHLLPIKPGSVEPCSDANIMGHFNPLGWNISDSPAPGTGTMDQYEIGDISGKFGMLNGLSLLEALHLDPDIQLTGPHSIAGRSLVVHYANGSRMRCADILAERHTEGQWIVAKALFNSTVTGAVQLRQQVFPDGSSNDVTLLVTLKSSERNTPSLSLFIFNGQCSNLRDVHNPFNMTSGDSRCSLVHPLSCVLGEISTRHGPVSLTHGQLFTDSIIQLSGDNTVVHKCLVLKNGDSVTACANILPESPTAEQKFPTRTDFSRYDFRRRVASVLQMDVARVTILPDSPSSVADGRCQEVNFMICGTVSPELLESVKTSEKMGIYRESDKCATSAVLRALPEVFLFGLMFAVACLLPSSVYS

Target ORF information:

RefSeq Version	XM_008317449.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis uncharacterized LOC103384079 (LOC103384079), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008317449.2

ORF Insert Sequence:

ATGCTCCTCC TGACTGCCAC CCTCGTGTTT CCTTTAATTG GTTCGATCTA CGGCACTCAG 
TTTAAAGCAA CTCTAAACAT GGCAGGAGTC ACCGGACAGG TGCAGTTTGA CTCCACATCC 
CAGACTGCCG CTGTTGCAGT TTCTGGTGCT GGATCCTGTG GTCTAGTGGA ACTGTTCCTC 
AGTGAGTTCC CCGTCATGTA TGGCCATTTT CCTGAACCTT GTTCGGAAGC AAATATCGGT 
CCCAAGGTTT TTTCCTTCAC AGCAGATCCC GCCTCATCGT CCACCGTCAA TGTGTCACAC 
CTCTTTGAAC GAAGACCAAA CCTGGACGAC TTTACTTTGT CCTTGAAGAC GTGTGACGAG 
ATCATGGTTT GTACAGTGGT AGGTCAAGGC AGATCATCCT TGACTTTTCA GGCTAGATTC 
ACTGGTCCAA TTGCCGGTAA TGTTTACATT CGAACAAACT CTGAACAAAG TAACGTCAGG 
CTTCTAGCAG ACCTGGTCAC AATCGGTCAA GTAAATGCCA CTAACACCAG TATCACTCTT 
CTGGGATCTA CAAGCACCGC TGCAAACTGT GATGTTCTGC TTGGAAGCTT GAAGGCTAGC 
ACTTTGTCAA GTTTGGGAAA GGTCAAAGTC GGAACACCTC TGGATCTTAA GAAATCCCGT 
CTGGACAACT GGGAAAACTG GAGTCTTCGA TTTCTTCTTT ACCACGCGGG CTCAAGTTAC 
AAGTGTGCTC GCGTTTATAA TGTCCCTGGT AAACAGGTGA CTGCTGTTAT TAATATGAAG 
GGGATTAAAG GGTATTTTAC CTTTAAACAA GCTTCATGTT TTGATGCAAC AGAGATGAGA 
GTTAACCTCA CCAACCTGCA GGGCAGAGTC GGTCCATATC ACGTCCACCT CTATCCGGTC 
CCTTCAGTCA GAACAAGCAA CAAAGAAAGT TTATGCTCCA ATGAAAATGT TGGTGGACAT 
TGGAATCCCT TTAAAATCAA GACTGCGGAC CCGACATATC CTAAAGGTCC TGGGTCAACA 
CATGACAAGT ACGAGGTTGG AGATCTGAGT ACGAGGCACG TGTCCCTCAC AGATATGAAT 
GACACTGACG TAATGTTTAC GGATTTCAAC CTTCCTCTGT TTGGACAAAA CAGTATTGTC 
GGTCGCTCAG TTGTTATACA CCAAACAGAC GGTTCCAGGT ATGTTTGCTC TAGCATCAGT 
TATCCTGGCA AAGTAGTTGT CGGCAGAGCC AGGTTTCAGA GTCATGTGGT TGGTGAGATC 
TGGTTCACCC AACTGGAAAA CAACCCTTTG TCCGATGTAT CCATCTTCAT GGATCTGGCT 
TATGGAAGTC CGACAACCAG CCCCACCATT AACCACCACT GGCATATTCA CCACTACCCT 
ATAAGCTCAG AGAGAGATGA TGACGAGAGG CGCTGCAGCA CAACAGAAGC CCACTGGAAC 
CCCTTCCACA TCGACACAGA AGACAGCAGC TATTTGCTAC ACTGTGGCCC AGCCAGACCT 
CTCTCCTGTG AGAGTGGAGA CCTGTCCAGC AAGCACAGAA CCATCGACCT CACCACCCAA 
GTTGGTTCAG TGCACGCCAA AAGCTTCTTC ACCGATGTCA CTTCCTGGTT GCAGGATGCA 
GGCGTTATTG GTCGTTCTGT GGTCATTCAT CAAGCCAACA GAGGAGGACC AAGAATCGCT 
TGTGCAAATG TCACCATGGT GCGAGAGCAC AAAGCCAGAC TGGGTAACTG GTTTGGTCCT 
GGACTGTCCG GAGGTCAAAT GCGGCTCGTT CAAGGTGCCG TGCAAGGACC CACGACTATC 
AACGTGTCTT TGATGAACCT CGACTCTCTG GCGGGGGGTT ACCACGTTCA CCTGCTGCCC 
ATCAAACCTG GCAGCGTGGA GCCCTGCTCT GATGCAAACA TCATGGGTCA TTTCAACCCA 
TTAGGCTGGA ACATATCGGA TTCTCCCGCA CCAGGAACTG GCACAATGGA CCAGTATGAA 
ATCGGAGACA TCAGTGGGAA GTTTGGAATG CTGAATGGTC TCAGCCTACT TGAAGCTCTG 
CACTTGGATC CTGATATTCA ATTAACTGGA CCCCACAGCA TTGCGGGAAG GTCCCTGGTG 
GTTCACTATG CCAATGGCTC GAGAATGAGG TGTGCTGACA TTTTAGCTGA AAGACATACT 
GAAGGACAAT GGATTGTTGC CAAGGCATTA TTCAACAGCA CAGTTACTGG GGCAGTACAA 
CTGCGTCAGC AGGTGTTTCC CGACGGGAGT AGTAATGATG TCACACTTCT GGTGACGCTG 
AAATCATCTG AACGGAATAC TCCTTCACTG TCACTGTTTA TCTTCAATGG CCAATGCAGC 
AACTTGAGAG ATGTTCATAA TCCCTTCAAC ATGACATCAG GAGACTCCAG GTGTTCATTA 
GTTCACCCTC TGAGCTGTGT GCTAGGGGAA ATATCCACCA GGCACGGTCC AGTCAGTCTA 
ACCCACGGTC AGCTGTTCAC TGACAGTATC ATCCAGCTGT CTGGAGACAA CACTGTGGTC 
CACAAATGTT TGGTGCTGAA AAATGGAGAT AGCGTCACAG CGTGTGCCAA CATTCTGCCA 
GAATCCCCAA CGGCAGAGCA GAAATTTCCT ACTCGGACAG ATTTCAGCAG ATACGATTTC 
CGTAGAAGAG TTGCAAGTGT CCTGCAGATG GACGTTGCGA GAGTCACCAT CTTGCCCGAC 
TCTCCGTCCT CTGTAGCAGA TGGAAGGTGT CAAGAGGTCA ACTTCATGAT ATGTGGAACT 
GTCAGCCCCG AACTCCTGGA GTCGGTGAAA ACCAGCGAGA AGATGGGTAT TTACAGAGAG 
TCTGACAAGT GTGCAACAAG TGCAGTTCTC CGTGCGCTGC CAGAAGTTTT TCTATTCGGC 
CTGATGTTTG CTGTCGCCTG CCTGCTGCCG TCTTCAGTTT ATTCATAG