CUPG1 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	CUPG1
Entrez Gene ID	101217558
Full Name	probable polygalacturonase At1g80170
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011657317.1	XP_011655619.1	probable polygalacturonase At1g80170
XM_011657317.2	XP_011655619.1	probable polygalacturonase At1g80170 isoform X1
NM_001305685.1	NP_001292614.1	probable polygalacturonase At1g80170 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
Li Z, Zhang Z, Yan P, Huang S, Fei Z, Lin K
BMC genomics12540(2011 Nov)

Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
Guo S, Zheng Y, Joung JG, Liu S, Zhang Z, Crasta OR, Sobral BW, Xu Y, Huang S, Fei Z
BMC genomics11384(2010 Jun)

The genome of the cucumber, Cucumis sativus L.
Huang S, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas WJ, Wang X, Xie B, Ni P, Ren Y, Zhu H, Li J, Lin K, Jin W, Fei Z, Li G, Staub J, Kilian A, van der Vossen EA, Wu Y, Guo J, He J, Jia Z, Ren Y, Tian G, Lu Y, Ruan J, Qian W, Wang M, Huang Q, Li B, Xuan Z, Cao J, Asan, Wu Z, Zhang J, Cai Q, Bai Y, Zhao B, Han Y, Li Y, Li X, Wang S, Shi Q, Liu S, Cho WK, Kim JY, Xu Y, Heller-Uszynska K, Miao H, Cheng Z, Zhang S, Wu J, Yang Y, Kang H, Li M, Liang H, Ren X, Shi Z, Wen M, Jian M, Yang H, Zhang G, Yang Z, Chen R, Liu S, Li J, Ma L, Liu H, Zhou Y, Zhao J, Fang X, Li G, Fang L, Li Y, Liu D, Zheng H, Zhang Y, Qin N, Li Z, Yang G, Yang S, Bolund L, Kristiansen K, Zheng H, Li S, Zhang X, Yang H, Wang J, Sun R, Zhang B, Jiang S, Wang J, Du Y, Li S
Nature genetics41(12)1275-81(2009 Dec)

An integrated genetic and cytogenetic map of the cucumber genome.
Ren Y, Zhang Z, Liu J, Staub JE, Han Y, Cheng Z, Li X, Lu J, Miao H, Kang H, Xie B, Gu X, Wang X, Du Y, Jin W, Huang S
PloS one4(6)e5795(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CUPG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CUPG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu03732	NM_001305685.1 Latest version!	Cucumis sativus probable polygalacturonase At1g80170 (CUPG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCu67369	XM_011657317.2 Latest version!	Cucumis sativus probable polygalacturonase At1g80170 (CUPG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OCu67369	XM_011657317.1	Cucumis sativus probable polygalacturonase At1g80170 (LOC101217558), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CUPG1 ( NM_001305685.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001292614.1 Cucumis sativus probable polygalacturonase At1g80170 (CUPG1), mRNA.

Price & Availability↑

CloneID	OCu03732
Clone ID Related Accession (Same CDS sequence)	NM_001305685.1
Accession Version	NM_001305685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cucumis sativus(cucumber)
Product	probable polygalacturonase At1g80170 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB035890.1. ##Evidence-Data-START## Transcript exon combination :: AB035890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02570546, SAMN02570552 [ECO:0000348] ##Evidence-Data-END##

ATGGATAAAT TATTTCTTCT TTCCCTTCTT GGGCTGTTTA TCATTGCCTA TGGAGTTTCA 
GGTGCTTTCG TGTCTGATAG CGACATTGAC ATGATTAAAG AACTTGAAAG TTTCGACATT 
GAAGAAGAGG ACGATGATGA CGTGTACTCG TACACAGTCC CGGTGTGGAG AAGTGAGCGT 
GGGAGCAAGA TTCTGATGAA TGTGGATAGT TTCGATGCGA TTGGAGATGG AGTAACAGAT 
GATACTATGG CTTTCAGAAA GGCTTGGGAG ACTGCCTGCA GCACTTCAAA ATCAGTTCTT 
TTGGTGCCTA AAGAAAAGAC ATATCTGGTG AATGCGACCA CTTTTAAAGG GCCATGCAAA 
GATGGTATGG TAATCCAGAT TGAAGGAACA ATAGTTGCAC CAGATGAACC AGAGCTATGG 
GATCCGAAGT TTCCGCGACA ATGGCTAGAA TTTACAAAAC TAAATGCAGC TACCTTTCAA 
GGAGATGGAG TTATCGACGG ATCTGGTGAG AAATGGTGGG CAGAATCCTG TAAAAAGAAC 
AAGTCCAGGC CTTGCAAAGG AGCACCAACA GCATTAACCA TTGACTCAAG TTCAAATATA 
AGAGTAAAAG GCCTTACCAT TCAAAACAGC CAACAGATGC ATTTCACCAT TGCACGGTCT 
GAAACTGTTA GAATTACGGG GGTAAGAGTG TCAGCTCCAG GAGATAGCCC CAATACTGAC 
GGGATCCATA TCACTCAATC AACTAATGTT GTAGTTCAAA ACTGCAAGAT TTCAACAGGT 
GATGACTGCA TCTCCATTGT TAATGCTAGC TCTGGCATCA AAATGAAGGG AATATCATGC 
GGACCAGGAC ATGGGATCAG CATTGGAAGT TTAGGGAAAG ACAACTCCAC TGGCATTGTG 
ACAAAGGTGG TGTTAGACAC AGCCTACCTC AGAGAGACAA CAAATGGTGT TAGAATCAAG 
ACTTGGCAGG GAGGTTCTGG ATATGTTCGT GCCGTACGCT TCGAAAATGT GAGAATGGAA 
GATGTTGAGA ACCCCATTAT CATCGATCAG TTCTACTGTG ACTCTCCTAC TACTTGTGAA 
AGTCAGACAT CAGCCGTGAA AATAAGCCAA ATCATGTACC GAAACATAAG TGGAACCACA 
ACGAGCAAGA ACGCAATGAA ATTCGCCTGT AGCGACAGTG TTCCATGCAG CAACATAATT 
CTAAGCAATG TCAACTTGGA GAAAACAGAT GGAACAGTGG AGACCTATTG CCACGCTGCT 
CAAGGCTTTG GCTTATGGTG TTGTGCACCC ATCGGCGAGA CTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292614.1
CDS	29..1336
Translation	MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSERGSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGLWCCAPIGETA

Target ORF information:

RefSeq Version	NM_001305685.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable polygalacturonase At1g80170 (CUPG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305685.1

ORF Insert Sequence:

ATGGATAAAT TATTTCTTCT TTCCCTTCTT GGGCTGTTTA TCATTGCCTA TGGAGTTTCA 
GGTGCTTTCG TGTCTGATAG CGACATTGAC ATGATTAAAG AACTTGAAAG TTTCGACATT 
GAAGAAGAGG ACGATGATGA CGTGTACTCG TACACAGTCC CGGTGTGGAG AAGTGAGCGT 
GGGAGCAAGA TTCTGATGAA TGTGGATAGT TTCGATGCGA TTGGAGATGG AGTAACAGAT 
GATACTATGG CTTTCAGAAA GGCTTGGGAG ACTGCCTGCA GCACTTCAAA ATCAGTTCTT 
TTGGTGCCTA AAGAAAAGAC ATATCTGGTG AATGCGACCA CTTTTAAAGG GCCATGCAAA 
GATGGTATGG TAATCCAGAT TGAAGGAACA ATAGTTGCAC CAGATGAACC AGAGCTATGG 
GATCCGAAGT TTCCGCGACA ATGGCTAGAA TTTACAAAAC TAAATGCAGC TACCTTTCAA 
GGAGATGGAG TTATCGACGG ATCTGGTGAG AAATGGTGGG CAGAATCCTG TAAAAAGAAC 
AAGTCCAGGC CTTGCAAAGG AGCACCAACA GCATTAACCA TTGACTCAAG TTCAAATATA 
AGAGTAAAAG GCCTTACCAT TCAAAACAGC CAACAGATGC ATTTCACCAT TGCACGGTCT 
GAAACTGTTA GAATTACGGG GGTAAGAGTG TCAGCTCCAG GAGATAGCCC CAATACTGAC 
GGGATCCATA TCACTCAATC AACTAATGTT GTAGTTCAAA ACTGCAAGAT TTCAACAGGT 
GATGACTGCA TCTCCATTGT TAATGCTAGC TCTGGCATCA AAATGAAGGG AATATCATGC 
GGACCAGGAC ATGGGATCAG CATTGGAAGT TTAGGGAAAG ACAACTCCAC TGGCATTGTG 
ACAAAGGTGG TGTTAGACAC AGCCTACCTC AGAGAGACAA CAAATGGTGT TAGAATCAAG 
ACTTGGCAGG GAGGTTCTGG ATATGTTCGT GCCGTACGCT TCGAAAATGT GAGAATGGAA 
GATGTTGAGA ACCCCATTAT CATCGATCAG TTCTACTGTG ACTCTCCTAC TACTTGTGAA 
AGTCAGACAT CAGCCGTGAA AATAAGCCAA ATCATGTACC GAAACATAAG TGGAACCACA 
ACGAGCAAGA ACGCAATGAA ATTCGCCTGT AGCGACAGTG TTCCATGCAG CAACATAATT 
CTAAGCAATG TCAACTTGGA GAAAACAGAT GGAACAGTGG AGACCTATTG CCACGCTGCT 
CAAGGCTTTG GCTTATGGTG TTGTGCACCC ATCGGCGAGA CTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUPG1 ( XM_011657317.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011655619.1 Cucumis sativus probable polygalacturonase At1g80170 (LOC101217558), mRNA.

Price & Availability↑

CloneID	OCu67369
Clone ID Related Accession (Same CDS sequence)	XM_011657317.1 , XM_011657317.2
Accession Version	XM_011657317.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	probable polygalacturonase At1g80170
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953807.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT TATTTCTTCT TTCCCTTCTT GGGCTGTTTA TCATTGCCTA TGGAGTTTCA 
GGTGCTTTCG TGTCTGATAG CGACATTGAC ATGATTAAAG AACTTGAAAG TTTCGACATT 
GAAGAAGAGG ACGATGATGA CGTGTACTCG TACACAGTCC CGGTGTGGAG AAGTGAGCGT 
GGGAGCAAGA TTCTGATGAA TGTGGATAGT TTCGATGCGA TTGGAGATGG AGTAACAGAT 
GATACTATGG CTTTCAGAAA GGCTTGGGAG ACTGCCTGCA GCACTTCAAA ATCAGTTCTT 
TTGGTGCCAA AAGAAAAGAC ATATCTGGTG AATGCGACCA CTTTTAAAGG GCCATGCAAA 
GATGGTATGG TAATCCAGAT TGAAGGAACA ATAGTTGCAC CAGATGAACC AGAGCTATGG 
GATCCGAAGT TTCCGCGACA ATGGCTAGAA TTTACAAAAC TAAATGCAGC TACCTTTCAA 
GGAGATGGAG TTATCGACGG ATCTGGTGAG AAATGGTGGG CAGAATCCTG TAAAAAGAAC 
AAGTCCAGGC CTTGCAAAGG AGCACCAACA GCATTAACCA TTGACTCAAG TTCAAATATA 
AGAGTAAAAG GCCTTACCAT TCAAAACAGC CAACAGATGC ATTTCACCAT TGCACGGTCT 
GAAACTGTTA GAATTACGGG GGTAAGAGTG TCAGCTCCAG GAGATAGCCC CAATACTGAC 
GGGATCCATA TCACTCAATC AACTAATGTT GTAGTTCAAA ACTGCAAGAT TTCAACAGGT 
GATGACTGCA TCTCCATTGT TAATGCTAGC TCTGGCATCA AAATGAAGGG AATATCATGC 
GGACCAGGAC ATGGGATCAG CATTGGAAGT TTAGGGAAAG ACAACTCCAC TGGCATTGTG 
ACAAAGGTGG TGTTAGACAC AGCCTACCTC AGAGAGACAA CAAATGGTGT TAGAATCAAG 
ACTTGGCAGG GAGGTTCTGG ATATGTTCGT GCCGTACGCT TCGAAAATGT GAGAATGGAA 
GATGTTGAGA ACCCCATTAT CATCGATCAG TTCTACTGTG ACTCTCCTAC TACTTGTGAA 
AGTCAGACAT CAGCCGTGAA AATAAGCCAA ATCATGTACC GAAACATAAG TGGAACCACA 
ACGAGCAAGA ACGCAATGAA ATTCGCCTGT AGCGACAGTG TTCCATGCAG CAACATAATT 
CTAAGCAATG TCAACTTGGA GAAAACAGAT GGAACAGTGG AGACCTATTG CCACGCTGCT 
CAAGGCTTTG GCTATGGTGT TGTGCACCCA TCGGCAGACT GCTTAACTTC CAGCGACAAT 
AACTTTGCAT CTTCAGATCA AACGCAAGTT CTGGAGCTGG AGACAGTGCA ACTCTCTACC 
GAACAGCACA TTGTTCATAC TGAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011655619.1
CDS	413..1822
Translation	MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSERGSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIVHTEL

Target ORF information:

RefSeq Version	XM_011657317.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable polygalacturonase At1g80170 (LOC101217558), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011657317.1

ORF Insert Sequence:

ATGGATAAAT TATTTCTTCT TTCCCTTCTT GGGCTGTTTA TCATTGCCTA TGGAGTTTCA 
GGTGCTTTCG TGTCTGATAG CGACATTGAC ATGATTAAAG AACTTGAAAG TTTCGACATT 
GAAGAAGAGG ACGATGATGA CGTGTACTCG TACACAGTCC CGGTGTGGAG AAGTGAGCGT 
GGGAGCAAGA TTCTGATGAA TGTGGATAGT TTCGATGCGA TTGGAGATGG AGTAACAGAT 
GATACTATGG CTTTCAGAAA GGCTTGGGAG ACTGCCTGCA GCACTTCAAA ATCAGTTCTT 
TTGGTGCCAA AAGAAAAGAC ATATCTGGTG AATGCGACCA CTTTTAAAGG GCCATGCAAA 
GATGGTATGG TAATCCAGAT TGAAGGAACA ATAGTTGCAC CAGATGAACC AGAGCTATGG 
GATCCGAAGT TTCCGCGACA ATGGCTAGAA TTTACAAAAC TAAATGCAGC TACCTTTCAA 
GGAGATGGAG TTATCGACGG ATCTGGTGAG AAATGGTGGG CAGAATCCTG TAAAAAGAAC 
AAGTCCAGGC CTTGCAAAGG AGCACCAACA GCATTAACCA TTGACTCAAG TTCAAATATA 
AGAGTAAAAG GCCTTACCAT TCAAAACAGC CAACAGATGC ATTTCACCAT TGCACGGTCT 
GAAACTGTTA GAATTACGGG GGTAAGAGTG TCAGCTCCAG GAGATAGCCC CAATACTGAC 
GGGATCCATA TCACTCAATC AACTAATGTT GTAGTTCAAA ACTGCAAGAT TTCAACAGGT 
GATGACTGCA TCTCCATTGT TAATGCTAGC TCTGGCATCA AAATGAAGGG AATATCATGC 
GGACCAGGAC ATGGGATCAG CATTGGAAGT TTAGGGAAAG ACAACTCCAC TGGCATTGTG 
ACAAAGGTGG TGTTAGACAC AGCCTACCTC AGAGAGACAA CAAATGGTGT TAGAATCAAG 
ACTTGGCAGG GAGGTTCTGG ATATGTTCGT GCCGTACGCT TCGAAAATGT GAGAATGGAA 
GATGTTGAGA ACCCCATTAT CATCGATCAG TTCTACTGTG ACTCTCCTAC TACTTGTGAA 
AGTCAGACAT CAGCCGTGAA AATAAGCCAA ATCATGTACC GAAACATAAG TGGAACCACA 
ACGAGCAAGA ACGCAATGAA ATTCGCCTGT AGCGACAGTG TTCCATGCAG CAACATAATT 
CTAAGCAATG TCAACTTGGA GAAAACAGAT GGAACAGTGG AGACCTATTG CCACGCTGCT 
CAAGGCTTTG GCTATGGTGT TGTGCACCCA TCGGCAGACT GCTTAACTTC CAGCGACAAT 
AACTTTGCAT CTTCAGATCA AACGCAAGTT CTGGAGCTGG AGACAGTGCA ACTCTCTACC 
GAACAGCACA TTGTTCATAC TGAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUPG1 ( XM_011657317.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011655619.1 Cucumis sativus probable polygalacturonase At1g80170 (CUPG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu67369
Clone ID Related Accession (Same CDS sequence)	XM_011657317.1 , XM_011657317.2
Accession Version	XM_011657317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	probable polygalacturonase At1g80170 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026659.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011657317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT TATTTCTTCT TTCCCTTCTT GGGCTGTTTA TCATTGCCTA TGGAGTTTCA 
GGTGCTTTCG TGTCTGATAG CGACATTGAC ATGATTAAAG AACTTGAAAG TTTCGACATT 
GAAGAAGAGG ACGATGATGA CGTGTACTCG TACACAGTCC CGGTGTGGAG AAGTGAGCGT 
GGGAGCAAGA TTCTGATGAA TGTGGATAGT TTCGATGCGA TTGGAGATGG AGTAACAGAT 
GATACTATGG CTTTCAGAAA GGCTTGGGAG ACTGCCTGCA GCACTTCAAA ATCAGTTCTT 
TTGGTGCCAA AAGAAAAGAC ATATCTGGTG AATGCGACCA CTTTTAAAGG GCCATGCAAA 
GATGGTATGG TAATCCAGAT TGAAGGAACA ATAGTTGCAC CAGATGAACC AGAGCTATGG 
GATCCGAAGT TTCCGCGACA ATGGCTAGAA TTTACAAAAC TAAATGCAGC TACCTTTCAA 
GGAGATGGAG TTATCGACGG ATCTGGTGAG AAATGGTGGG CAGAATCCTG TAAAAAGAAC 
AAGTCCAGGC CTTGCAAAGG AGCACCAACA GCATTAACCA TTGACTCAAG TTCAAATATA 
AGAGTAAAAG GCCTTACCAT TCAAAACAGC CAACAGATGC ATTTCACCAT TGCACGGTCT 
GAAACTGTTA GAATTACGGG GGTAAGAGTG TCAGCTCCAG GAGATAGCCC CAATACTGAC 
GGGATCCATA TCACTCAATC AACTAATGTT GTAGTTCAAA ACTGCAAGAT TTCAACAGGT 
GATGACTGCA TCTCCATTGT TAATGCTAGC TCTGGCATCA AAATGAAGGG AATATCATGC 
GGACCAGGAC ATGGGATCAG CATTGGAAGT TTAGGGAAAG ACAACTCCAC TGGCATTGTG 
ACAAAGGTGG TGTTAGACAC AGCCTACCTC AGAGAGACAA CAAATGGTGT TAGAATCAAG 
ACTTGGCAGG GAGGTTCTGG ATATGTTCGT GCCGTACGCT TCGAAAATGT GAGAATGGAA 
GATGTTGAGA ACCCCATTAT CATCGATCAG TTCTACTGTG ACTCTCCTAC TACTTGTGAA 
AGTCAGACAT CAGCCGTGAA AATAAGCCAA ATCATGTACC GAAACATAAG TGGAACCACA 
ACGAGCAAGA ACGCAATGAA ATTCGCCTGT AGCGACAGTG TTCCATGCAG CAACATAATT 
CTAAGCAATG TCAACTTGGA GAAAACAGAT GGAACAGTGG AGACCTATTG CCACGCTGCT 
CAAGGCTTTG GCTATGGTGT TGTGCACCCA TCGGCAGACT GCTTAACTTC CAGCGACAAT 
AACTTTGCAT CTTCAGATCA AACGCAAGTT CTGGAGCTGG AGACAGTGCA ACTCTCTACC 
GAACAGCACA TTGTTCATAC TGAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011655619.1
CDS	747..2156
Translation	MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSERGSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIVHTEL

Target ORF information:

RefSeq Version	XM_011657317.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable polygalacturonase At1g80170 (CUPG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011657317.2

ORF Insert Sequence:

ATGGATAAAT TATTTCTTCT TTCCCTTCTT GGGCTGTTTA TCATTGCCTA TGGAGTTTCA 
GGTGCTTTCG TGTCTGATAG CGACATTGAC ATGATTAAAG AACTTGAAAG TTTCGACATT 
GAAGAAGAGG ACGATGATGA CGTGTACTCG TACACAGTCC CGGTGTGGAG AAGTGAGCGT 
GGGAGCAAGA TTCTGATGAA TGTGGATAGT TTCGATGCGA TTGGAGATGG AGTAACAGAT 
GATACTATGG CTTTCAGAAA GGCTTGGGAG ACTGCCTGCA GCACTTCAAA ATCAGTTCTT 
TTGGTGCCAA AAGAAAAGAC ATATCTGGTG AATGCGACCA CTTTTAAAGG GCCATGCAAA 
GATGGTATGG TAATCCAGAT TGAAGGAACA ATAGTTGCAC CAGATGAACC AGAGCTATGG 
GATCCGAAGT TTCCGCGACA ATGGCTAGAA TTTACAAAAC TAAATGCAGC TACCTTTCAA 
GGAGATGGAG TTATCGACGG ATCTGGTGAG AAATGGTGGG CAGAATCCTG TAAAAAGAAC 
AAGTCCAGGC CTTGCAAAGG AGCACCAACA GCATTAACCA TTGACTCAAG TTCAAATATA 
AGAGTAAAAG GCCTTACCAT TCAAAACAGC CAACAGATGC ATTTCACCAT TGCACGGTCT 
GAAACTGTTA GAATTACGGG GGTAAGAGTG TCAGCTCCAG GAGATAGCCC CAATACTGAC 
GGGATCCATA TCACTCAATC AACTAATGTT GTAGTTCAAA ACTGCAAGAT TTCAACAGGT 
GATGACTGCA TCTCCATTGT TAATGCTAGC TCTGGCATCA AAATGAAGGG AATATCATGC 
GGACCAGGAC ATGGGATCAG CATTGGAAGT TTAGGGAAAG ACAACTCCAC TGGCATTGTG 
ACAAAGGTGG TGTTAGACAC AGCCTACCTC AGAGAGACAA CAAATGGTGT TAGAATCAAG 
ACTTGGCAGG GAGGTTCTGG ATATGTTCGT GCCGTACGCT TCGAAAATGT GAGAATGGAA 
GATGTTGAGA ACCCCATTAT CATCGATCAG TTCTACTGTG ACTCTCCTAC TACTTGTGAA 
AGTCAGACAT CAGCCGTGAA AATAAGCCAA ATCATGTACC GAAACATAAG TGGAACCACA 
ACGAGCAAGA ACGCAATGAA ATTCGCCTGT AGCGACAGTG TTCCATGCAG CAACATAATT 
CTAAGCAATG TCAACTTGGA GAAAACAGAT GGAACAGTGG AGACCTATTG CCACGCTGCT 
CAAGGCTTTG GCTATGGTGT TGTGCACCCA TCGGCAGACT GCTTAACTTC CAGCGACAAT 
AACTTTGCAT CTTCAGATCA AACGCAAGTT CTGGAGCTGG AGACAGTGCA ACTCTCTACC 
GAACAGCACA TTGTTCATAC TGAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
BMC genomics12540(2011 Nov)
Li Z,Zhang Z,Yan P,Huang S,Fei Z,Lin K

Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
BMC genomics11384(2010 Jun)
Guo S,Zheng Y,Joung JG,Liu S,Zhang Z,Crasta OR,Sobral BW,Xu Y,Huang S,Fei Z

The genome of the cucumber, Cucumis sativus L.
Nature genetics41(12)1275-81(2009 Dec)
Huang S,Li R,Zhang Z,Li L,Gu X,Fan W,Lucas WJ,Wang X,Xie B,Ni P,Ren Y,Zhu H,Li J,Lin K,Jin W,Fei Z,Li G,Staub J,Kilian A,van der Vossen EA,Wu Y,Guo J,He J,Jia Z,Ren Y,Tian G,Lu Y,Ruan J,Qian W,Wang M,Huang Q,Li B,Xuan Z,Cao J,Asan,Wu Z,Zhang J,Cai Q,Bai Y,Zhao B,Han Y,Li Y,Li X,Wang S,Shi Q,Liu S,Cho WK,Kim JY,Xu Y,Heller-Uszynska K,Miao H,Cheng Z,Zhang S,Wu J,Yang Y,Kang H,Li M,Liang H,Ren X,Shi Z,Wen M,Jian M,Yang H,Zhang G,Yang Z,Chen R,Liu S,Li J,Ma L,Liu H,Zhou Y,Zhao J,Fang X,Li G,Fang L,Li Y,Liu D,Zheng H,Zhang Y,Qin N,Li Z,Yang G,Yang S,Bolund L,Kristiansen K,Zheng H,Li S,Zhang X,Yang H,Wang J,Sun R,Zhang B,Jiang S,Wang J,Du Y,Li S

An integrated genetic and cytogenetic map of the cucumber genome.
PloS one4(6)e5795(2009 Jun)
Ren Y,Zhang Z,Liu J,Staub JE,Han Y,Cheng Z,Li X,Lu J,Miao H,Kang H,Xie B,Gu X,Wang X,Du Y,Jin W,Huang S

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CUPG1 cDNA ORF clone, Cucumis sativus(cucumber)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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