EF1 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	EF1
Entrez Gene ID	101215193
Full Name	elongation factor 1-alpha
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004138916.2	XP_004138964.1	elongation factor 1-alpha
XM_004138916.3	XP_004138964.1	elongation factor 1-alpha

Pathways from BioSystems
Gene Ontology

The following EF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu56441	XM_004138916.3 Latest version!	Cucumis sativus elongation factor 1-alpha (EF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OCu56441	XM_004138916.2	Cucumis sativus elongation factor 1-alpha (LOC101215193), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EF1 ( XM_004138916.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004138964.1 Cucumis sativus elongation factor 1-alpha (LOC101215193), mRNA.

Price & Availability↑

CloneID	OCu56441
Clone ID Related Accession (Same CDS sequence)	XM_004138916.2 , XM_004138916.3
Accession Version	XM_004138916.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	elongation factor 1-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953804.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 23, 2015 this sequence version replaced XM_004138916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAG AAAAGACTCA TATCAACATC GTGGTTATTG GCCATGTCGA CTCTGGAAAG 
TCGACCACCA CTGGTCATCT TATCTACAAG CTTGGAGGGA TTGACAAGCG TGTGATTGAG 
AGATTCGAGA AGGAAGCCGC TGAGATGAAC AAGAGGTCAT TCAAGTATGC TTGGGTGCTC 
GACAAACTTA AGGCAGAGCG TGAACGTGGT ATTACCATTG ACATTGCCCT GTGGAAGTTT 
GAGACCACCA AGTACTACTG CACAGTCATT GATGCTCCCG GACATCGTGA CTTTATCAAG 
AACATGATTA CTGGAACCTC ACAGGCTGAC TGTGCTGTCC TCATTATTGA CTCCACCACT 
GGTGGTTTTG AGGCTGGTAT TTCCAAGGAT GGTCAGACCC GTGAGCACGC TCTTCTTGCT 
TTCACCCTTG GTGTCAAGCA AATGATCTGC TGCTGCAACA AGATGGATGC CACCACACCC 
AAATACTCCA AGGCAAGGTA CGATGAAATT GTGAAGGAAG TCTCATCATA CCTCAAGAAG 
GTCGGATACA ACCCAGACAA AATCCCCTTC GTTCCCATCT CTGGTTTCGA GGGTGACAAC 
ATGATTGAGA GGTCCACCAA CCTTGATTGG TACAAGGGAC CAACCCTTCT TGAGGCTCTT 
GACTTGATCT CTGAGCCCAA GAGGCCCTCA GACAAGCCCC TCCGTCTCCC ACTTCAGGAC 
GTTTACAAGA TTGGTGGTAT TGGAACTGTC CCTGTTGGAC GTGTTGAAAC TGGTGTCCTC 
AAGCCTGGTA TGGTTGTTAC CTTCGGACCA ACTGGACTGA CCACTGAAGT TAAGTCTGTT 
GAAATGCACC ACGAGTCTCT CCCAGAAGCT TTGCCAGGTG ACAATGTTGG CTTCAACGTC 
AAGAACGTTG CTGTCAAGGA TCTCAAGCGT GGTTTCGTTG CCTCAAACTC CAAGGATGAC 
CCTGCCAAGG AAGCTGCTAA CTTTACATCT CAGGTTATCA TCATGAACCA CCCTGGTCAG 
ATTGGTAATG GTTATGCTCC AGTCCTTGAT TGCCACACTT CCCACATTGC TGTTAAGTTC 
GCTGAGATCC TCACCAAAAT CGATCGTCGA TCTGCTAAGG AGCTTGAGAA GGAGCCCAAG 
TTCTTGAAGA ATGGAGATGC CGGTATGGTT AAGATGATTC CAACCAAACC CATGGTTGTG 
GAGACCTTCT CTACATACCC ACCATTGGGT CGATTTGCTG TCCGTGATAT GCGTCAAACC 
GTTGCTGTTG GTGTTATCAA GAGTGTGGAG AAGAAGGACC CAACAGGAGC CAAGGTCACC 
AAGTCCGCTG TGAAGAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004138964.1
CDS	133..1476
Translation	MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDLISEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPTGLTTEVKSVEMHHESLPEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEILTKIDRRSAKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSTYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTGAKVTKSAVKKK

Target ORF information:

RefSeq Version	XM_004138916.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus elongation factor 1-alpha (LOC101215193), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004138916.2

ORF Insert Sequence:

ATGGGTAAAG AAAAGACTCA TATCAACATC GTGGTTATTG GCCATGTCGA CTCTGGAAAG 
TCGACCACCA CTGGTCATCT TATCTACAAG CTTGGAGGGA TTGACAAGCG TGTGATTGAG 
AGATTCGAGA AGGAAGCCGC TGAGATGAAC AAGAGGTCAT TCAAGTATGC TTGGGTGCTC 
GACAAACTTA AGGCAGAGCG TGAACGTGGT ATTACCATTG ACATTGCCCT GTGGAAGTTT 
GAGACCACCA AGTACTACTG CACAGTCATT GATGCTCCCG GACATCGTGA CTTTATCAAG 
AACATGATTA CTGGAACCTC ACAGGCTGAC TGTGCTGTCC TCATTATTGA CTCCACCACT 
GGTGGTTTTG AGGCTGGTAT TTCCAAGGAT GGTCAGACCC GTGAGCACGC TCTTCTTGCT 
TTCACCCTTG GTGTCAAGCA AATGATCTGC TGCTGCAACA AGATGGATGC CACCACACCC 
AAATACTCCA AGGCAAGGTA CGATGAAATT GTGAAGGAAG TCTCATCATA CCTCAAGAAG 
GTCGGATACA ACCCAGACAA AATCCCCTTC GTTCCCATCT CTGGTTTCGA GGGTGACAAC 
ATGATTGAGA GGTCCACCAA CCTTGATTGG TACAAGGGAC CAACCCTTCT TGAGGCTCTT 
GACTTGATCT CTGAGCCCAA GAGGCCCTCA GACAAGCCCC TCCGTCTCCC ACTTCAGGAC 
GTTTACAAGA TTGGTGGTAT TGGAACTGTC CCTGTTGGAC GTGTTGAAAC TGGTGTCCTC 
AAGCCTGGTA TGGTTGTTAC CTTCGGACCA ACTGGACTGA CCACTGAAGT TAAGTCTGTT 
GAAATGCACC ACGAGTCTCT CCCAGAAGCT TTGCCAGGTG ACAATGTTGG CTTCAACGTC 
AAGAACGTTG CTGTCAAGGA TCTCAAGCGT GGTTTCGTTG CCTCAAACTC CAAGGATGAC 
CCTGCCAAGG AAGCTGCTAA CTTTACATCT CAGGTTATCA TCATGAACCA CCCTGGTCAG 
ATTGGTAATG GTTATGCTCC AGTCCTTGAT TGCCACACTT CCCACATTGC TGTTAAGTTC 
GCTGAGATCC TCACCAAAAT CGATCGTCGA TCTGCTAAGG AGCTTGAGAA GGAGCCCAAG 
TTCTTGAAGA ATGGAGATGC CGGTATGGTT AAGATGATTC CAACCAAACC CATGGTTGTG 
GAGACCTTCT CTACATACCC ACCATTGGGT CGATTTGCTG TCCGTGATAT GCGTCAAACC 
GTTGCTGTTG GTGTTATCAA GAGTGTGGAG AAGAAGGACC CAACAGGAGC CAAGGTCACC 
AAGTCCGCTG TGAAGAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EF1 ( XM_004138916.3 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004138964.1 Cucumis sativus elongation factor 1-alpha (EF1), mRNA.

Price & Availability↑

CloneID	OCu56441
Clone ID Related Accession (Same CDS sequence)	XM_004138916.2 , XM_004138916.3
Accession Version	XM_004138916.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	elongation factor 1-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026656.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_004138916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAG AAAAGACTCA TATCAACATC GTGGTTATTG GCCATGTCGA CTCTGGAAAG 
TCGACCACCA CTGGTCATCT TATCTACAAG CTTGGAGGGA TTGACAAGCG TGTGATTGAG 
AGATTCGAGA AGGAAGCCGC TGAGATGAAC AAGAGGTCAT TCAAGTATGC TTGGGTGCTC 
GACAAACTTA AGGCAGAGCG TGAACGTGGT ATTACCATTG ACATTGCCCT GTGGAAGTTT 
GAGACCACCA AGTACTACTG CACAGTCATT GATGCTCCCG GACATCGTGA CTTTATCAAG 
AACATGATTA CTGGAACCTC ACAGGCTGAC TGTGCTGTCC TCATTATTGA CTCCACCACT 
GGTGGTTTTG AGGCTGGTAT TTCCAAGGAT GGTCAGACCC GTGAGCACGC TCTTCTTGCT 
TTCACCCTTG GTGTCAAGCA AATGATCTGC TGCTGCAACA AGATGGATGC CACCACACCC 
AAATACTCCA AGGCAAGGTA CGATGAAATT GTGAAGGAAG TCTCATCATA CCTCAAGAAG 
GTCGGATACA ACCCAGACAA AATCCCCTTC GTTCCCATCT CTGGTTTCGA GGGTGACAAC 
ATGATTGAGA GGTCCACCAA CCTTGATTGG TACAAGGGAC CAACCCTTCT TGAGGCTCTT 
GACTTGATCT CTGAGCCCAA GAGGCCCTCA GACAAGCCCC TCCGTCTCCC ACTTCAGGAC 
GTTTACAAGA TTGGTGGTAT TGGAACTGTC CCTGTTGGAC GTGTTGAAAC TGGTGTCCTC 
AAGCCTGGTA TGGTTGTTAC CTTCGGACCA ACTGGACTGA CCACTGAAGT TAAGTCTGTT 
GAAATGCACC ACGAGTCTCT CCCAGAAGCT TTGCCAGGTG ACAATGTTGG CTTCAACGTC 
AAGAACGTTG CTGTCAAGGA TCTCAAGCGT GGTTTCGTTG CCTCAAACTC CAAGGATGAC 
CCTGCCAAGG AAGCTGCTAA CTTTACATCT CAGGTTATCA TCATGAACCA CCCTGGTCAG 
ATTGGTAATG GTTATGCTCC AGTCCTTGAT TGCCACACTT CCCACATTGC TGTTAAGTTC 
GCTGAGATCC TCACCAAAAT CGATCGTCGA TCTGCTAAGG AGCTTGAGAA GGAGCCCAAG 
TTCTTGAAGA ATGGAGATGC CGGTATGGTT AAGATGATTC CAACCAAACC CATGGTTGTG 
GAGACCTTCT CTACATACCC ACCATTGGGT CGATTTGCTG TCCGTGATAT GCGTCAAACC 
GTTGCTGTTG GTGTTATCAA GAGTGTGGAG AAGAAGGACC CAACAGGAGC CAAGGTCACC 
AAGTCCGCTG TGAAGAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004138964.1
CDS	157..1500
Translation	MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDLISEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPTGLTTEVKSVEMHHESLPEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEILTKIDRRSAKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSTYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTGAKVTKSAVKKK

Target ORF information:

RefSeq Version	XM_004138916.3
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus elongation factor 1-alpha (EF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004138916.3

ORF Insert Sequence:

ATGGGTAAAG AAAAGACTCA TATCAACATC GTGGTTATTG GCCATGTCGA CTCTGGAAAG 
TCGACCACCA CTGGTCATCT TATCTACAAG CTTGGAGGGA TTGACAAGCG TGTGATTGAG 
AGATTCGAGA AGGAAGCCGC TGAGATGAAC AAGAGGTCAT TCAAGTATGC TTGGGTGCTC 
GACAAACTTA AGGCAGAGCG TGAACGTGGT ATTACCATTG ACATTGCCCT GTGGAAGTTT 
GAGACCACCA AGTACTACTG CACAGTCATT GATGCTCCCG GACATCGTGA CTTTATCAAG 
AACATGATTA CTGGAACCTC ACAGGCTGAC TGTGCTGTCC TCATTATTGA CTCCACCACT 
GGTGGTTTTG AGGCTGGTAT TTCCAAGGAT GGTCAGACCC GTGAGCACGC TCTTCTTGCT 
TTCACCCTTG GTGTCAAGCA AATGATCTGC TGCTGCAACA AGATGGATGC CACCACACCC 
AAATACTCCA AGGCAAGGTA CGATGAAATT GTGAAGGAAG TCTCATCATA CCTCAAGAAG 
GTCGGATACA ACCCAGACAA AATCCCCTTC GTTCCCATCT CTGGTTTCGA GGGTGACAAC 
ATGATTGAGA GGTCCACCAA CCTTGATTGG TACAAGGGAC CAACCCTTCT TGAGGCTCTT 
GACTTGATCT CTGAGCCCAA GAGGCCCTCA GACAAGCCCC TCCGTCTCCC ACTTCAGGAC 
GTTTACAAGA TTGGTGGTAT TGGAACTGTC CCTGTTGGAC GTGTTGAAAC TGGTGTCCTC 
AAGCCTGGTA TGGTTGTTAC CTTCGGACCA ACTGGACTGA CCACTGAAGT TAAGTCTGTT 
GAAATGCACC ACGAGTCTCT CCCAGAAGCT TTGCCAGGTG ACAATGTTGG CTTCAACGTC 
AAGAACGTTG CTGTCAAGGA TCTCAAGCGT GGTTTCGTTG CCTCAAACTC CAAGGATGAC 
CCTGCCAAGG AAGCTGCTAA CTTTACATCT CAGGTTATCA TCATGAACCA CCCTGGTCAG 
ATTGGTAATG GTTATGCTCC AGTCCTTGAT TGCCACACTT CCCACATTGC TGTTAAGTTC 
GCTGAGATCC TCACCAAAAT CGATCGTCGA TCTGCTAAGG AGCTTGAGAA GGAGCCCAAG 
TTCTTGAAGA ATGGAGATGC CGGTATGGTT AAGATGATTC CAACCAAACC CATGGTTGTG 
GAGACCTTCT CTACATACCC ACCATTGGGT CGATTTGCTG TCCGTGATAT GCGTCAAACC 
GTTGCTGTTG GTGTTATCAA GAGTGTGGAG AAGAAGGACC CAACAGGAGC CAAGGTCACC 
AAGTCCGCTG TGAAGAAGAA ATAG