CS-ACS2 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	CS-ACS2
Entrez Gene ID	101212728
Full Name	1-aminocyclopropane-1-carboxylate synthase-like
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001280660.1	NP_001267589.1	1-aminocyclopropane-1-carboxylate synthase-like

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A putative positive feedback regulation mechanism in CsACS2 expression suggests a modified model for sex determination in cucumber (Cucumis sativus L.).
Li Z, Wang S, Tao Q, Pan J, Si L, Gong Z, Cai R
Journal of experimental botany63(12)4475-84(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest a positive feedback regulation mechanism for CsACS2, and a modified 'one-hormone hypothesis' for sex determination in cucumber is proposed.
Title: A putative positive feedback regulation mechanism in CsACS2 expression suggests a modified model for sex determination in cucumber (Cucumis sativus L.).

The following CS-ACS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CS-ACS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu36647	NM_001280660.1 Latest version!	Cucumis sativus 1-aminocyclopropane-1-carboxylate synthase-like (CS-ACS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CS-ACS2 ( NM_001280660.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001267589.1 Cucumis sativus 1-aminocyclopropane-1-carboxylate synthase-like (CS-ACS2), mRNA.

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CloneID	OCu36647
Clone ID Related Accession (Same CDS sequence)	NM_001280660.1
Accession Version	NM_001280660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cucumis sativus(cucumber)
Product	1-aminocyclopropane-1-carboxylate synthase-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB006804.1. On Jul 23, 2013 this sequence version replaced XM_004146484.1. ##Evidence-Data-START## Transcript exon combination :: AB006804.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04530041 [ECO:0000348] ##Evidence-Data-END##

ATGGTACTTG TTTCCAGCAA TGATAATGGA AATGCAAAGA AAGTTTTGTC CAAATTAGCC 
AAAGGAAATG GGCATGGTGA AGATTCCTCT TATTTTGATG GGTGGAAGGC TTATGATTCT 
GATCCTTTTC ATCCTATTAT CAATCCTCGT GGTGTTATTC AAATGGGTTT AGCTGAAAAT 
CAGCTTTCTT TTGAATTTGT GGAGAAATGG ATGAAAAACA ATCCAAGAGC TTCAATTTGC 
AGTGTTGAAG GAATTGATGA GTTCAAGGAT ATTGCTATCT TTCAAGATTA TCATGGATTG 
CCAGAGTTTA GAAACGCTGT AGCGAACTTC ATGGGAAAAG TGAGAGGGAA CCGAGTCAAA 
TTTGATCCTG ACCGAGTTGT GATGAGTGGT GGAGCAACTG GAGCTCACGA AACCATGGCG 
TTTTGTTTGG CTGATCCTGG TGAAGCATTT CTTGTTCCTG TTCCTTATTA TCCAGGATTC 
GACAGAGATT TAAGGTGGAG GACAGGAGTG GAAATAGTTC CAGTGATATG TGAAAGTTCA 
AACAATTTCA AATTAACAAG AGAAGCCTTA GAGACAGCAT ATGAAGAAGC TCAAAAATCC 
AACATAAAAA TCAAAGGTCT ACTCATAACG AATCCTTCAA ATCCACTCGG AACGGTTTAC 
GATAGACAAA CACTTGAAAC TGCTGTATCG TTTATCAACG AAAAGAATAT CCACTTAGTG 
TGCGACGAAA TCTACGCTGC CACTGTCTTT GCAGAGCCCG GTTTCATTAG TATCTCTGAA 
GTGATCGACA ACAGTGACAT TGAATGCGAT AGAAATCTTG TCCATGTCGT TTATAGCCTA 
TCCAAAGACA TGGGATTCCC TGGATTTCGT GTCGGTATTA TCTACTCTTA TAACGACGCT 
GTTGTTGCAT GCGCTCGTAA GATGTCAAGC TTTGGCCTCG TCTCCTCGCA AACTCAATAT 
TTGATTGCAT CGATGTTGTT GGATGATGTG TTTGTCGACA ACTTTCTTGC TGGGAGTGCT 
GAAAAACTCG CTGCGCGTCA TCGAAACTTC ACAAAAGGGC TTGCTCAGGT TGGAATTGGA 
TATTTGAAAG GAAGTGGAGG ATTATTTTTA TGGATGGATT TGCGTCATCT TTTGAAAGAG 
AAGACATTGG AAGCAGAAAT GGCATTATGG AAAGTGATTA TCAATGAAGT TAAACTAAAT 
GTTTCACCAG GTTCTTCATT CCATTGCTCA GAACCAGGAT GGTTTAGGGT TTGTTTTGCA 
AATATGGATG ACAATACAAT GGACATTTCC ATCACCAGGA TTAGAAACTT CGTGCTTCAA 
AACAAGGAGG TAACAACTAA GGTCAAGAAA CAAAAATTCT GCTGGCGTCA AAGCAGCCTC 
GAACTTCGAT TGTCATCTCG AAGACTCGAA GACATCATGT CACCACACTC ACCGTTGCCT 
CAATCCCCTA TGCATCGTGC AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267589.1
CDS	63..1532
Translation	MVLVSSNDNGNAKKVLSKLAKGNGHGEDSSYFDGWKAYDSDPFHPIINPRGVIQMGLAENQLSFEFVEKWMKNNPRASICSVEGIDEFKDIAIFQDYHGLPEFRNAVANFMGKVRGNRVKFDPDRVVMSGGATGAHETMAFCLADPGEAFLVPVPYYPGFDRDLRWRTGVEIVPVICESSNNFKLTREALETAYEEAQKSNIKIKGLLITNPSNPLGTVYDRQTLETAVSFINEKNIHLVCDEIYAATVFAEPGFISISEVIDNSDIECDRNLVHVVYSLSKDMGFPGFRVGIIYSYNDAVVACARKMSSFGLVSSQTQYLIASMLLDDVFVDNFLAGSAEKLAARHRNFTKGLAQVGIGYLKGSGGLFLWMDLRHLLKEKTLEAEMALWKVIINEVKLNVSPGSSFHCSEPGWFRVCFANMDDNTMDISITRIRNFVLQNKEVTTKVKKQKFCWRQSSLELRLSSRRLEDIMSPHSPLPQSPMHRATT

Target ORF information:

RefSeq Version	NM_001280660.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus 1-aminocyclopropane-1-carboxylate synthase-like (CS-ACS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280660.1

ORF Insert Sequence:

ATGGTACTTG TTTCCAGCAA TGATAATGGA AATGCAAAGA AAGTTTTGTC CAAATTAGCC 
AAAGGAAATG GGCATGGTGA AGATTCCTCT TATTTTGATG GGTGGAAGGC TTATGATTCT 
GATCCTTTTC ATCCTATTAT CAATCCTCGT GGTGTTATTC AAATGGGTTT AGCTGAAAAT 
CAGCTTTCTT TTGAATTTGT GGAGAAATGG ATGAAAAACA ATCCAAGAGC TTCAATTTGC 
AGTGTTGAAG GAATTGATGA GTTCAAGGAT ATTGCTATCT TTCAAGATTA TCATGGATTG 
CCAGAGTTTA GAAACGCTGT AGCGAACTTC ATGGGAAAAG TGAGAGGGAA CCGAGTCAAA 
TTTGATCCTG ACCGAGTTGT GATGAGTGGT GGAGCAACTG GAGCTCACGA AACCATGGCG 
TTTTGTTTGG CTGATCCTGG TGAAGCATTT CTTGTTCCTG TTCCTTATTA TCCAGGATTC 
GACAGAGATT TAAGGTGGAG GACAGGAGTG GAAATAGTTC CAGTGATATG TGAAAGTTCA 
AACAATTTCA AATTAACAAG AGAAGCCTTA GAGACAGCAT ATGAAGAAGC TCAAAAATCC 
AACATAAAAA TCAAAGGTCT ACTCATAACG AATCCTTCAA ATCCACTCGG AACGGTTTAC 
GATAGACAAA CACTTGAAAC TGCTGTATCG TTTATCAACG AAAAGAATAT CCACTTAGTG 
TGCGACGAAA TCTACGCTGC CACTGTCTTT GCAGAGCCCG GTTTCATTAG TATCTCTGAA 
GTGATCGACA ACAGTGACAT TGAATGCGAT AGAAATCTTG TCCATGTCGT TTATAGCCTA 
TCCAAAGACA TGGGATTCCC TGGATTTCGT GTCGGTATTA TCTACTCTTA TAACGACGCT 
GTTGTTGCAT GCGCTCGTAA GATGTCAAGC TTTGGCCTCG TCTCCTCGCA AACTCAATAT 
TTGATTGCAT CGATGTTGTT GGATGATGTG TTTGTCGACA ACTTTCTTGC TGGGAGTGCT 
GAAAAACTCG CTGCGCGTCA TCGAAACTTC ACAAAAGGGC TTGCTCAGGT TGGAATTGGA 
TATTTGAAAG GAAGTGGAGG ATTATTTTTA TGGATGGATT TGCGTCATCT TTTGAAAGAG 
AAGACATTGG AAGCAGAAAT GGCATTATGG AAAGTGATTA TCAATGAAGT TAAACTAAAT 
GTTTCACCAG GTTCTTCATT CCATTGCTCA GAACCAGGAT GGTTTAGGGT TTGTTTTGCA 
AATATGGATG ACAATACAAT GGACATTTCC ATCACCAGGA TTAGAAACTT CGTGCTTCAA 
AACAAGGAGG TAACAACTAA GGTCAAGAAA CAAAAATTCT GCTGGCGTCA AAGCAGCCTC 
GAACTTCGAT TGTCATCTCG AAGACTCGAA GACATCATGT CACCACACTC ACCGTTGCCT 
CAATCCCCTA TGCATCGTGC AACAACTTAA