LIG4 cDNA ORF clone, Cuculus canorus(common cuckoo)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIG4
Entrez Gene ID	104062976
Full Name	DNA ligase 4
Gene Type	protein-coding
Organism	Cuculus canorus(common cuckoo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009565119.1	XP_009563414.1	DNA ligase 4

Pathways from BioSystems
Gene Ontology

The following LIG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu48190	XM_009565119.1 Latest version!	Cuculus canorus ligase IV, DNA, ATP-dependent (LIG4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIG4 ( XM_009565119.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009563414.1 Cuculus canorus ligase IV, DNA, ATP-dependent (LIG4), mRNA.

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CloneID	OCu48190
Clone ID Related Accession (Same CDS sequence)	XM_009565119.1
Accession Version	XM_009565119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	DNA ligase 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009245776.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA CACCTGCTTC GCAGTCTTCT AAAAAAACGG TGGCTTCTCG CGTGCCTTTT 
GCAGATCTGT GTTCTACTCT GGAGCGGATA CAGACGTGTA AATCTCGTCC AGAGAAAATC 
AAGTATTTCA AGGAATTTCT AGATTCCTGG AGAAAATTCC ATGATGCTCT TCATCAGAAA 
GAGAAAGACG TCACAGATTC TTTTTACCCA GCCATGCGGC TTATTCTTCC ACAGTTGGAA 
AGAGAAAGGA TGGCCTATGG GATTAAAGAA ACTATGCTTG CAAAGCTGTA TATTGAACTG 
CTTAATTTAC CAAAAGATGG AAAAGATGCT TCAAAGCTTT TAAATTACAG GACACCTGCT 
GGCACACGTG GAGATGCTGG AGACTTTGCA ATGGTTGCAT ACTTCGTGTT AAAACCTAGG 
AGCCCAAAAC AAGGCAGACT GACGATAGAA CAAGTTAATG AACATTTAGA TGTGATAGCT 
AATAATAATG CTGCTAAAAA CAAGGGTCTG TTAAAGAAAA GTCTTCTCCA GTTAATTACC 
CAGAGCACAG CACTGGAACA AAAATGGCTT ATCCGGATGA TTATAAAGGA TCTAAAGCTT 
GGTGTTAGCG AACGCACTAT ATTTTCAGTT TTTCATCCTG ATGCTGCTGA ATTGCACAAT 
GTCACAACTG ATTTGGAAAA AGTTTGCAGA CAGCTGCATG ATCCCTCTGT CTCACTGAGT 
GATGTTTCTA TCATGTTGTT TTCTGCCTTT AAACCAATGC TTGCTGCTAT TGCTGATGTC 
AAGCAAATTG AGAAACAAAT GAATAACCAG GTATTCTACA TAGAAACTAA GTTGGATGGT 
GAGCGTATGC AGATGCACAA AGATGGAGAT GTGTATAAGT ATTTTTCCCG GAATGGCTTT 
GACTATACTC AGCAGTTTGG TGCTTCCCCT CTTGATGGTT CATTAACACC ATTTATTCAT 
AATGTGTTTA AGAGCAATAT ACAAAATTGC ATTCTTGATG GTGAAATGAT GGCTTACAAT 
CCTGAGACAC AAACCTTTAT GCAGAAAGGG AACAAATTTG ACATAAAAAG AATGGTGGAG 
GACTCTGATC TGCAGACCTG CTTTTGTGTG TTTGATGTTT TGATGGTTAA CAATCAGAAG 
TTGGGTCAGG AAACACTAAG CAAAAGGTAT GAAATCTTAA GTAGCGTATT TTCCCCAGTA 
ACAGGCAGGT TACATGTTGT ACCAAAAAAA AATGCTAGAA TGAGAAAAGA AGTAATTGAT 
GCTTTAAATG AAGCAATAGA TAACAGAGAG GAAGGGATTA TGGTGAAAGA TCCCATGTCT 
ACCTACAAGC CTGACAAACG TGGGGAAGGC TGGTTAAAAA TCAAGCCAGA ATACGTCAAT 
GGATTGATGG ATGAACTAGA CCTTTTAATT GTTGGTGGCT ACTGGGGGAA GGGCTCACGT 
GGTGGAATGA TGTCTCATTT TCTGTGTGCT GTTGCAGAGA AACCCCGTCC AAATGAAAAA 
CCTACTGTTT TCCACTCTAT TTGTCGGGTT GGCTCTGGCT ATACTATGAA GGAATTGTAT 
GATCTAGGCT TGAAACTGTC TAAACACTGG AAGCCCTATG ATAGGAAGGA CCCTCCCTCT 
AACATTTTGT GTGGAACTGA AAAACCTGAA ATGTACATTG AACCTTGCAA CTCTGTCATA 
GTTCAGGTCA AGGCAGCCGA GATTGTTAAC AGTGACATGT ACAAAACTGA CTGTACTTTG 
AGATTCCCCC GAATTGAGAA GATAAGAGAG GACAAAGAAT GGTATGAGTG CATGACTTTA 
GATATATTAG AAGATCTCAG AAGCAAAGCA GAAGGGAAGC TGGCATCAAA GCATCTTCAT 
ATAGATGAGT ATGATGAGCC ACAAGAGAAA AAAAGAAAGA CCGTGTCAAA GGTGAAGAAG 
GTAATTGGAA TAGCTGAGCA ATTTAAAGCT CCTGATCTTT CTAATGTAAG CAAGGTTTCA 
AATATATTTG AAGATGTGGA GTTTTGTGTT ATGACAGGAA TGGGAAAGTA CTCAAAGTCA 
GAACTAGAAA GCAGAATAGC CGAATATGGT GGCAGTGTGG TACAGAATCC AGGGCCAGAG 
ACATACTGTG TCATTGTAGG AGCTGAGAAT GTGAGAGTGA AAAACATCAT TGCTTCCAAC 
AAATACGATG TGGTTAGGGC AGAGTGGCTT CTTCAGTGTT TTCAGACTAA AATGCTGGTG 
CCTTGGCAGC CAGCCTTTAT GATTCACATG TCTCCTGACA CGAAAGAACA TTTTGCTCGT 
GAGTATGATT GTTACGGAGA CAGCTACACC GCAGAGACAG ATGTCGCACA ACTCAAGGAA 
GTGTTCTCAA GAATGAAAGA CAATAAGATG ATGCCTTTGG ATGTGATTGC AGTGTTAGAG 
GAACGTTATT CATGGAACAG CTGTCCACTC AGTATATTCA GAGGAAACAC TGTTTATGTG 
GACTGTTATG CTATTGTTAA TGATCCCAGA ACCAAAATCC ATGGAACGAT ACTATCAATT 
AGAGCTTTGG AGCTCCGTTT TTATGGTGCA AAAGTGGTCT TGTGCCTTGA AGAGGGCGTC 
TCTCATGTTG TTATAGGAGA AGATCATTCC CGAGTTAAAG AGATGAAAGC ACTCAGGAGA 
ACATTTGGGA AGAAATTTAA AATTGTGTCT GAGCTGTGGG TAACAGTGTC AGTGGAGGAA 
GGAGTCCTAA AGAATGAAAA TCAGTACTTA ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009563414.1
CDS	1..2736
Translation	MASTPASQSSKKTVASRVPFADLCSTLERIQTCKSRPEKIKYFKEFLDSWRKFHDALHQKEKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPKDGKDASKLLNYRTPAGTRGDAGDFAMVAYFVLKPRSPKQGRLTIEQVNEHLDVIANNNAAKNKGLLKKSLLQLITQSTALEQKWLIRMIIKDLKLGVSERTIFSVFHPDAAELHNVTTDLEKVCRQLHDPSVSLSDVSIMLFSAFKPMLAAIADVKQIEKQMNNQVFYIETKLDGERMQMHKDGDVYKYFSRNGFDYTQQFGASPLDGSLTPFIHNVFKSNIQNCILDGEMMAYNPETQTFMQKGNKFDIKRMVEDSDLQTCFCVFDVLMVNNQKLGQETLSKRYEILSSVFSPVTGRLHVVPKKNARMRKEVIDALNEAIDNREEGIMVKDPMSTYKPDKRGEGWLKIKPEYVNGLMDELDLLIVGGYWGKGSRGGMMSHFLCAVAEKPRPNEKPTVFHSICRVGSGYTMKELYDLGLKLSKHWKPYDRKDPPSNILCGTEKPEMYIEPCNSVIVQVKAAEIVNSDMYKTDCTLRFPRIEKIREDKEWYECMTLDILEDLRSKAEGKLASKHLHIDEYDEPQEKKRKTVSKVKKVIGIAEQFKAPDLSNVSKVSNIFEDVEFCVMTGMGKYSKSELESRIAEYGGSVVQNPGPETYCVIVGAENVRVKNIIASNKYDVVRAEWLLQCFQTKMLVPWQPAFMIHMSPDTKEHFAREYDCYGDSYTAETDVAQLKEVFSRMKDNKMMPLDVIAVLEERYSWNSCPLSIFRGNTVYVDCYAIVNDPRTKIHGTILSIRALELRFYGAKVVLCLEEGVSHVVIGEDHSRVKEMKALRRTFGKKFKIVSELWVTVSVEEGVLKNENQYLI

Target ORF information:

RefSeq Version	XM_009565119.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus ligase IV, DNA, ATP-dependent (LIG4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009565119.1

ORF Insert Sequence:

ATGGCTTCCA CACCTGCTTC GCAGTCTTCT AAAAAAACGG TGGCTTCTCG CGTGCCTTTT 
GCAGATCTGT GTTCTACTCT GGAGCGGATA CAGACGTGTA AATCTCGTCC AGAGAAAATC 
AAGTATTTCA AGGAATTTCT AGATTCCTGG AGAAAATTCC ATGATGCTCT TCATCAGAAA 
GAGAAAGACG TCACAGATTC TTTTTACCCA GCCATGCGGC TTATTCTTCC ACAGTTGGAA 
AGAGAAAGGA TGGCCTATGG GATTAAAGAA ACTATGCTTG CAAAGCTGTA TATTGAACTG 
CTTAATTTAC CAAAAGATGG AAAAGATGCT TCAAAGCTTT TAAATTACAG GACACCTGCT 
GGCACACGTG GAGATGCTGG AGACTTTGCA ATGGTTGCAT ACTTCGTGTT AAAACCTAGG 
AGCCCAAAAC AAGGCAGACT GACGATAGAA CAAGTTAATG AACATTTAGA TGTGATAGCT 
AATAATAATG CTGCTAAAAA CAAGGGTCTG TTAAAGAAAA GTCTTCTCCA GTTAATTACC 
CAGAGCACAG CACTGGAACA AAAATGGCTT ATCCGGATGA TTATAAAGGA TCTAAAGCTT 
GGTGTTAGCG AACGCACTAT ATTTTCAGTT TTTCATCCTG ATGCTGCTGA ATTGCACAAT 
GTCACAACTG ATTTGGAAAA AGTTTGCAGA CAGCTGCATG ATCCCTCTGT CTCACTGAGT 
GATGTTTCTA TCATGTTGTT TTCTGCCTTT AAACCAATGC TTGCTGCTAT TGCTGATGTC 
AAGCAAATTG AGAAACAAAT GAATAACCAG GTATTCTACA TAGAAACTAA GTTGGATGGT 
GAGCGTATGC AGATGCACAA AGATGGAGAT GTGTATAAGT ATTTTTCCCG GAATGGCTTT 
GACTATACTC AGCAGTTTGG TGCTTCCCCT CTTGATGGTT CATTAACACC ATTTATTCAT 
AATGTGTTTA AGAGCAATAT ACAAAATTGC ATTCTTGATG GTGAAATGAT GGCTTACAAT 
CCTGAGACAC AAACCTTTAT GCAGAAAGGG AACAAATTTG ACATAAAAAG AATGGTGGAG 
GACTCTGATC TGCAGACCTG CTTTTGTGTG TTTGATGTTT TGATGGTTAA CAATCAGAAG 
TTGGGTCAGG AAACACTAAG CAAAAGGTAT GAAATCTTAA GTAGCGTATT TTCCCCAGTA 
ACAGGCAGGT TACATGTTGT ACCAAAAAAA AATGCTAGAA TGAGAAAAGA AGTAATTGAT 
GCTTTAAATG AAGCAATAGA TAACAGAGAG GAAGGGATTA TGGTGAAAGA TCCCATGTCT 
ACCTACAAGC CTGACAAACG TGGGGAAGGC TGGTTAAAAA TCAAGCCAGA ATACGTCAAT 
GGATTGATGG ATGAACTAGA CCTTTTAATT GTTGGTGGCT ACTGGGGGAA GGGCTCACGT 
GGTGGAATGA TGTCTCATTT TCTGTGTGCT GTTGCAGAGA AACCCCGTCC AAATGAAAAA 
CCTACTGTTT TCCACTCTAT TTGTCGGGTT GGCTCTGGCT ATACTATGAA GGAATTGTAT 
GATCTAGGCT TGAAACTGTC TAAACACTGG AAGCCCTATG ATAGGAAGGA CCCTCCCTCT 
AACATTTTGT GTGGAACTGA AAAACCTGAA ATGTACATTG AACCTTGCAA CTCTGTCATA 
GTTCAGGTCA AGGCAGCCGA GATTGTTAAC AGTGACATGT ACAAAACTGA CTGTACTTTG 
AGATTCCCCC GAATTGAGAA GATAAGAGAG GACAAAGAAT GGTATGAGTG CATGACTTTA 
GATATATTAG AAGATCTCAG AAGCAAAGCA GAAGGGAAGC TGGCATCAAA GCATCTTCAT 
ATAGATGAGT ATGATGAGCC ACAAGAGAAA AAAAGAAAGA CCGTGTCAAA GGTGAAGAAG 
GTAATTGGAA TAGCTGAGCA ATTTAAAGCT CCTGATCTTT CTAATGTAAG CAAGGTTTCA 
AATATATTTG AAGATGTGGA GTTTTGTGTT ATGACAGGAA TGGGAAAGTA CTCAAAGTCA 
GAACTAGAAA GCAGAATAGC CGAATATGGT GGCAGTGTGG TACAGAATCC AGGGCCAGAG 
ACATACTGTG TCATTGTAGG AGCTGAGAAT GTGAGAGTGA AAAACATCAT TGCTTCCAAC 
AAATACGATG TGGTTAGGGC AGAGTGGCTT CTTCAGTGTT TTCAGACTAA AATGCTGGTG 
CCTTGGCAGC CAGCCTTTAT GATTCACATG TCTCCTGACA CGAAAGAACA TTTTGCTCGT 
GAGTATGATT GTTACGGAGA CAGCTACACC GCAGAGACAG ATGTCGCACA ACTCAAGGAA 
GTGTTCTCAA GAATGAAAGA CAATAAGATG ATGCCTTTGG ATGTGATTGC AGTGTTAGAG 
GAACGTTATT CATGGAACAG CTGTCCACTC AGTATATTCA GAGGAAACAC TGTTTATGTG 
GACTGTTATG CTATTGTTAA TGATCCCAGA ACCAAAATCC ATGGAACGAT ACTATCAATT 
AGAGCTTTGG AGCTCCGTTT TTATGGTGCA AAAGTGGTCT TGTGCCTTGA AGAGGGCGTC 
TCTCATGTTG TTATAGGAGA AGATCATTCC CGAGTTAAAG AGATGAAAGC ACTCAGGAGA 
ACATTTGGGA AGAAATTTAA AATTGTGTCT GAGCTGTGGG TAACAGTGTC AGTGGAGGAA 
GGAGTCCTAA AGAATGAAAA TCAGTACTTA ATTTAA