SMURF1 cDNA ORF clone, Cuculus canorus(common cuckoo)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMURF1
Entrez Gene ID	104062539
Full Name	SMAD specific E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Cuculus canorus(common cuckoo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009564608.1	XP_009562903.1	E3 ubiquitin-protein ligase SMURF1

Pathways from BioSystems
Gene Ontology

The following SMURF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMURF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu47751	XM_009564608.1 Latest version!	Cuculus canorus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMURF1 ( XM_009564608.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009562903.1 Cuculus canorus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), mRNA.

Price & Availability↑

CloneID	OCu47751
Clone ID Related Accession (Same CDS sequence)	XM_009564608.1
Accession Version	XM_009564608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	E3 ubiquitin-protein ligase SMURF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009245754.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAGACTG TGTTATGTGC CAAGAACCTT GCAAAGAAAG ATTTCTTCAG ACTTCCTGAC 
CCATTTGCGA AAATAGTTGT TGATGGGTCA GGTCAGTGCC ATTCAACTGA CACTGTGAAG 
AACACGTTGG ACCCCAAATG GAACCAGCAT TATGATCTAT ATGTTGGGAA GACAGATTCC 
ATAACCATCA GTGTATGGAA CCATAAAAAA ATCCACAAGA AGCAGGGAGC TGGCTTCCTG 
GGGTGTGTCC GACTCCTCTC CAATGCCATC AGCAGGCTAA AAGATACTGG ATACCAACGT 
TTGGATCTAT GCAAACTAAA TCCCACAGAT ACCGATGCTG TGCGAGGCCA AATAGTGGTC 
AGTTTACAGA CACGGGACAG AATAGGCACA GGAGGCTCTG TAGTGGACTG TAGAGGGCTT 
TTGGAGAATG AAGGAACGGT TTATGAAGAT TCTGGACCAG GAAGGCCACT GAGCTGTTTT 
ATGGAAGAAC CAGCACCGTA TACAGACACT ACAGGGGCTG CTGGCGGAGG CAACTGTCGC 
TTTGTAGAGT CCCCCAGTCA AGATCAGAGG CTTCAGGCAC AGCGACTTCG GACTCCTGAA 
GTCCGAGGGC ACACACAGAC ACCTCAGAAC AGGCCGCATG GTCACCAGTC GCCTGACCTA 
CCGGAAGGCT ACGAACAAAG AACAACGGTA CAGGGACAGG TTTATTTTTT GCACACACAG 
ACTGGAGTTA GCACGTGGCA TGATCCAAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT 
GATGAACTAG GACCTTTGCC TCCAGGCTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA 
TATTTTGTAG ATCATAACAA CAGAACCACA CAATTCACAG ATCCACGACT ACATCACATC 
ATGAACCATC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG 
GGGTCACTAG AAGATGGTGA GGAGTTGCCT GCACAAAGAT ATGAAAGAGA CTTGGTACAA 
AAGTTGAAAG TTCTTAGACA TGAGCTCTCT CTTCAGCAAC CACAGGCTGG TCACTGCCGC 
ATTGAAGTAT CCAGGGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG 
CCAAAGGACT TGAAAAAGAG GCTTATGGTG AAATTTAGAG GAGAGGAAGG CCTGGATTAT 
GGAGGAGTAG CTAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCCTACTAT 
GGACTCTTCC AGTACTCCAC AGATAATATT TACATGCTGC AAATCAACCC AGACTCTTCT 
ATCAATCCCG ACCATTTGTC TTATTTCCAT TTTGTTGGTC GGATAATGGG TTTGGCTGTG 
TTCCATGGAC ACTATATCAA TGGGGGTTTC ACAGTGCCCT TTTACAAACA GCTTCTGGGG 
AAGCCCATTC AACTCTCTGA TCTGGAATCT GTTGACCCAG AATTACATAA AAGTTTAGTC 
TGGATCTTAG AGAATGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAC 
GCTTTTGGGC GGATTTTACA GCATGAGCTC AAACCAAATG GCAGGAATAT TCCAGTGACA 
GAAGAGAACA AGAAAGAATA TGTCAGGCTG TATGTAAACT GGCGATTTAT GAGAGGCATC 
GAGGCACAGT TCCTGGCACT GCAGAAGGGT TTTAATGAAC TCATTCCTCA ACATCTTCTT 
AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG 
AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGACAGCAA TATTGTGAAG 
TGGTTCTGGC AAGCAGTGGA AACATTTGAT GAAGAAAGAA GAGCAAGGCT GCTGCAGTTT 
GTGACGGGTT CAACGCGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG CGCTGCAGGA 
CCCAGACTGT TTACCATCCA TTTAATAGAT GCAAACACAG ACAACCTTCC AAAAGCCCAC 
ACTTGCTTTA ACCGGATTGA CATTCCGCCT TATGAGTCAT ATGAGAAGCT TTATGAGAAG 
CTGTTGACAG CTGTGGAAGA GACATGTGGG TTTGCTGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009562903.1
CDS	1..2145
Translation	METVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPTDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDTTGAAGGGNCRFVESPSQDQRLQAQRLRTPEVRGHTQTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQQPLPAPNEGSLEDGEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCMADSNIVKWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_009564608.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009564608.1

ORF Insert Sequence:

ATGGAGACTG TGTTATGTGC CAAGAACCTT GCAAAGAAAG ATTTCTTCAG ACTTCCTGAC 
CCATTTGCGA AAATAGTTGT TGATGGGTCA GGTCAGTGCC ATTCAACTGA CACTGTGAAG 
AACACGTTGG ACCCCAAATG GAACCAGCAT TATGATCTAT ATGTTGGGAA GACAGATTCC 
ATAACCATCA GTGTATGGAA CCATAAAAAA ATCCACAAGA AGCAGGGAGC TGGCTTCCTG 
GGGTGTGTCC GACTCCTCTC CAATGCCATC AGCAGGCTAA AAGATACTGG ATACCAACGT 
TTGGATCTAT GCAAACTAAA TCCCACAGAT ACCGATGCTG TGCGAGGCCA AATAGTGGTC 
AGTTTACAGA CACGGGACAG AATAGGCACA GGAGGCTCTG TAGTGGACTG TAGAGGGCTT 
TTGGAGAATG AAGGAACGGT TTATGAAGAT TCTGGACCAG GAAGGCCACT GAGCTGTTTT 
ATGGAAGAAC CAGCACCGTA TACAGACACT ACAGGGGCTG CTGGCGGAGG CAACTGTCGC 
TTTGTAGAGT CCCCCAGTCA AGATCAGAGG CTTCAGGCAC AGCGACTTCG GACTCCTGAA 
GTCCGAGGGC ACACACAGAC ACCTCAGAAC AGGCCGCATG GTCACCAGTC GCCTGACCTA 
CCGGAAGGCT ACGAACAAAG AACAACGGTA CAGGGACAGG TTTATTTTTT GCACACACAG 
ACTGGAGTTA GCACGTGGCA TGATCCAAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT 
GATGAACTAG GACCTTTGCC TCCAGGCTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA 
TATTTTGTAG ATCATAACAA CAGAACCACA CAATTCACAG ATCCACGACT ACATCACATC 
ATGAACCATC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG 
GGGTCACTAG AAGATGGTGA GGAGTTGCCT GCACAAAGAT ATGAAAGAGA CTTGGTACAA 
AAGTTGAAAG TTCTTAGACA TGAGCTCTCT CTTCAGCAAC CACAGGCTGG TCACTGCCGC 
ATTGAAGTAT CCAGGGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG 
CCAAAGGACT TGAAAAAGAG GCTTATGGTG AAATTTAGAG GAGAGGAAGG CCTGGATTAT 
GGAGGAGTAG CTAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCCTACTAT 
GGACTCTTCC AGTACTCCAC AGATAATATT TACATGCTGC AAATCAACCC AGACTCTTCT 
ATCAATCCCG ACCATTTGTC TTATTTCCAT TTTGTTGGTC GGATAATGGG TTTGGCTGTG 
TTCCATGGAC ACTATATCAA TGGGGGTTTC ACAGTGCCCT TTTACAAACA GCTTCTGGGG 
AAGCCCATTC AACTCTCTGA TCTGGAATCT GTTGACCCAG AATTACATAA AAGTTTAGTC 
TGGATCTTAG AGAATGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAC 
GCTTTTGGGC GGATTTTACA GCATGAGCTC AAACCAAATG GCAGGAATAT TCCAGTGACA 
GAAGAGAACA AGAAAGAATA TGTCAGGCTG TATGTAAACT GGCGATTTAT GAGAGGCATC 
GAGGCACAGT TCCTGGCACT GCAGAAGGGT TTTAATGAAC TCATTCCTCA ACATCTTCTT 
AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG 
AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGACAGCAA TATTGTGAAG 
TGGTTCTGGC AAGCAGTGGA AACATTTGAT GAAGAAAGAA GAGCAAGGCT GCTGCAGTTT 
GTGACGGGTT CAACGCGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG CGCTGCAGGA 
CCCAGACTGT TTACCATCCA TTTAATAGAT GCAAACACAG ACAACCTTCC AAAAGCCCAC 
ACTTGCTTTA ACCGGATTGA CATTCCGCCT TATGAGTCAT ATGAGAAGCT TTATGAGAAG 
CTGTTGACAG CTGTGGAAGA GACATGTGGG TTTGCTGTGG AGTGA