Smurf1 cDNA ORF clone, Cricetulus griseus(Chinese hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smurf1
Entrez Gene ID	100765164
Full Name	SMAD specific E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Cricetulus griseus(Chinese hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027440589.1	XP_027296390.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_027413618.1	XP_027269419.1	E3 ubiquitin-protein ligase SMURF1 isoform X4
XM_016966968.1	XP_016822457.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_016978516.1	XP_016834005.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_027413616.1	XP_027269417.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_027413617.1	XP_027269418.1	E3 ubiquitin-protein ligase SMURF1 isoform X3

Pathways from BioSystems
Gene Ontology

The following Smurf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smurf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCr218799	XM_027440589.1 Latest version!	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCr233610	XM_027413616.1 Latest version!	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCr233611	XM_027413617.1 Latest version!	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCr233612	XM_027413618.1 Latest version!	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCr24268	XM_016978516.1 Latest version!	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCr38728	XM_016966968.1 Latest version!	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smurf1 ( XM_027440589.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_027296390.1 Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr218799
Clone ID Related Accession (Same CDS sequence)	XM_027440589.1
Accession Version	XM_027440589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-17
Organism	Cricetulus griseus(Chinese hamster)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003614494.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cricetulus griseus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGAGTCTGG TCACAGAGTT CCAGGCCAGC CTGGGCTGCA TGCTGAGACT ACGTGGTGAT 
CCACATAAGA AGCTTGTATT ATGTGCCAAG AACCTTGCAA AGAAAGACTT CTTCAGACTC 
CCTGACCCCT TTGCCAAGAT TGTTGTGGAC GGCTCTGGGC AGTGCCACTC AACTGACACT 
GTGAAAAACA CTCTGGACCC AAAGTGGAAC CAGCATTATG ACCTGTACGT TGGGAAAACC 
GACTCTATAA CGATCAGTGT GTGGAACCAC AAGAAGATCC ACAAGAAGCA GGGGGCTGGC 
TTCCTCGGCT GCGTGCGGCT GCTCTCCAAT GCCATCAGCA GATTGAAAGA CACTGGCTAC 
CAGCGTTTGG ATCTATGCAA ACTAAATCCC TCAGATACTG ATGCGGTTCG TGGCCAAATA 
GTGGTCAGTT TACAGACCCG AGACAGAATA GGCAGTGGAG GGTCGGTGGT GGACTGCAGG 
GGACTGCTAG AAAATGAAGG AACAGTGTAT GAAGACTCAG GCCCGGGAAG GCCGCTCAGC 
TGCCTCATGG AGGAACCTGC CCCATATACA GATGGTACCG GTGCAGCCGC TGGAGGAGGG 
AACTGCAGGT TTGTGGAGTC TCCGAGCCAA GACCAGAGAC TCCAGGTACA GCGACTCCGA 
AATCCTGAGG TTCGAGGGTC CCTACAGACG CCCCAGAACC GACCACATGG CCACCAGTCA 
CCAGAGTTGC CTGAAGGCTA TGAGCAAAGG ACGACAGTAC AGGGACAAGT TTACTTTTTG 
CACACACAGA CTGGAGTCAG CACGTGGCAT GACCCCAGGA TTCCCAGAGA CCTTAATAGT 
GTGAACTGTG ATGAACTTGG GCCGCTGCCT CCAGGCTGGG AAGTCAGAAG TACAGTGTCG 
GGAAGAATCT ATTTTGTAGA TCACAATAAT AGGACAACCC AGTTTACAGA CCCACGGCTT 
CACCACATCA TGAATCACCA GTGCCAACTC AAGGAGCCCA GCCAGCCGCT GCAGTTGCCC 
AGTGAGGGCT CCATGGACGA TGAGGAGCTT CCTGCCCAGA GATATGAGAG AGATTTAGTT 
CAGAAGCTCA AAGTTCTCAG GCACGAACTC TCTCTTCAGC AGCCCCAAGC TGGCCATTGT 
CGAATAGAAG TTTCCAGAGA AGAAATATTT GAGGAGTCAT ATCGCCAGAT CATGAAGATG 
CGGCCCAAGG ACCTGAAGAA GCGCCTGATG GTGAAGTTCC GAGGGGAGGA AGGTCTGGAT 
TATGGCGGAG TGGCACGGGA GTGGCTGTAT TTGTTGTGCC ATGAAATGTT GAATCCGTAT 
TATGGACTCT TCCAGTATTC CACGGACAAT ATTTACACTC TGCAAATCAA CCCAGATTCT 
TCTGTCAACC CTGACCATCT GTCTTACTTC CACTTTGTGG GTCGCATCAT GGGTCTGGCT 
GTGTTCCATG GGCACTACAT AAATGGGGGC TTCACAGTCC CATTCTACAA GCAGCTCCTG 
GGAAAACCCA TCCAGCTGTC GGATCTGGAG TCAGTGGACC CAGAACTGCA CAAGAGTTTG 
GTGTGGATTC TAGAGAATGA CATCACACCA GTGCTGGACC ATACCTTCTG TGTGGAGCAC 
AATGCTTTTG GGCGGATCCT TCAGCACGAA CTGAAACCCA ATGGCAGAAA TGTGCCTGTC 
ACTGAGGAAA ACAAGAAGGA ATATGTCCGG TTGTATGTAA ACTGGAGGTT TATGAGAGGA 
ATTGAAGCCC AGTTCTTAGC TCTTCAGAAA GGGTTTAATG AACTCATTCC CCAACACTTA 
CTGAAGCCCT TTGACCAGAA GGAACTGGAG CTGATAATAG GTGGGCTGGA TAAGATAGAC 
CTGAATGACT GGAAGTCAAA CACACGCCTG AAGCATTGTG TGGCCGACAG CAACATTGTG 
AGGTGGTTCT GGCAGGCAGT GGAGACCTTC GATGAGGAGA GGAGGGCCAG ACTCCTGCAG 
TTCGTGACCG GGTCCACACG AGTTCCTCTC CAAGGCTTCA AGGCTCTGCA AGGCGCAGCA 
GGGCCTCGAC TCTTCACCAT CCACCTGATA GATGCCAACA CAGACAACTT GCCCAAGGCT 
CATACCTGCT TTAACCGGAT TGACATCCCG CCCTATGAGT CCTATGAGAA GCTCTATGAG 
AAGCTGCTCA CAGCTGTGGA GGAGACCTGT GGCTTTGCTG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027296390.1
CDS	1..2208
Translation	MSLVTEFQASLGCMLRLRGDPHKKLVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGSGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLQVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSMDDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSVNPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_027440589.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027440589.1

ORF Insert Sequence:

ATGAGTCTGG TCACAGAGTT CCAGGCCAGC CTGGGCTGCA TGCTGAGACT ACGTGGTGAT 
CCACATAAGA AGCTTGTATT ATGTGCCAAG AACCTTGCAA AGAAAGACTT CTTCAGACTC 
CCTGACCCCT TTGCCAAGAT TGTTGTGGAC GGCTCTGGGC AGTGCCACTC AACTGACACT 
GTGAAAAACA CTCTGGACCC AAAGTGGAAC CAGCATTATG ACCTGTACGT TGGGAAAACC 
GACTCTATAA CGATCAGTGT GTGGAACCAC AAGAAGATCC ACAAGAAGCA GGGGGCTGGC 
TTCCTCGGCT GCGTGCGGCT GCTCTCCAAT GCCATCAGCA GATTGAAAGA CACTGGCTAC 
CAGCGTTTGG ATCTATGCAA ACTAAATCCC TCAGATACTG ATGCGGTTCG TGGCCAAATA 
GTGGTCAGTT TACAGACCCG AGACAGAATA GGCAGTGGAG GGTCGGTGGT GGACTGCAGG 
GGACTGCTAG AAAATGAAGG AACAGTGTAT GAAGACTCAG GCCCGGGAAG GCCGCTCAGC 
TGCCTCATGG AGGAACCTGC CCCATATACA GATGGTACCG GTGCAGCCGC TGGAGGAGGG 
AACTGCAGGT TTGTGGAGTC TCCGAGCCAA GACCAGAGAC TCCAGGTACA GCGACTCCGA 
AATCCTGAGG TTCGAGGGTC CCTACAGACG CCCCAGAACC GACCACATGG CCACCAGTCA 
CCAGAGTTGC CTGAAGGCTA TGAGCAAAGG ACGACAGTAC AGGGACAAGT TTACTTTTTG 
CACACACAGA CTGGAGTCAG CACGTGGCAT GACCCCAGGA TTCCCAGAGA CCTTAATAGT 
GTGAACTGTG ATGAACTTGG GCCGCTGCCT CCAGGCTGGG AAGTCAGAAG TACAGTGTCG 
GGAAGAATCT ATTTTGTAGA TCACAATAAT AGGACAACCC AGTTTACAGA CCCACGGCTT 
CACCACATCA TGAATCACCA GTGCCAACTC AAGGAGCCCA GCCAGCCGCT GCAGTTGCCC 
AGTGAGGGCT CCATGGACGA TGAGGAGCTT CCTGCCCAGA GATATGAGAG AGATTTAGTT 
CAGAAGCTCA AAGTTCTCAG GCACGAACTC TCTCTTCAGC AGCCCCAAGC TGGCCATTGT 
CGAATAGAAG TTTCCAGAGA AGAAATATTT GAGGAGTCAT ATCGCCAGAT CATGAAGATG 
CGGCCCAAGG ACCTGAAGAA GCGCCTGATG GTGAAGTTCC GAGGGGAGGA AGGTCTGGAT 
TATGGCGGAG TGGCACGGGA GTGGCTGTAT TTGTTGTGCC ATGAAATGTT GAATCCGTAT 
TATGGACTCT TCCAGTATTC CACGGACAAT ATTTACACTC TGCAAATCAA CCCAGATTCT 
TCTGTCAACC CTGACCATCT GTCTTACTTC CACTTTGTGG GTCGCATCAT GGGTCTGGCT 
GTGTTCCATG GGCACTACAT AAATGGGGGC TTCACAGTCC CATTCTACAA GCAGCTCCTG 
GGAAAACCCA TCCAGCTGTC GGATCTGGAG TCAGTGGACC CAGAACTGCA CAAGAGTTTG 
GTGTGGATTC TAGAGAATGA CATCACACCA GTGCTGGACC ATACCTTCTG TGTGGAGCAC 
AATGCTTTTG GGCGGATCCT TCAGCACGAA CTGAAACCCA ATGGCAGAAA TGTGCCTGTC 
ACTGAGGAAA ACAAGAAGGA ATATGTCCGG TTGTATGTAA ACTGGAGGTT TATGAGAGGA 
ATTGAAGCCC AGTTCTTAGC TCTTCAGAAA GGGTTTAATG AACTCATTCC CCAACACTTA 
CTGAAGCCCT TTGACCAGAA GGAACTGGAG CTGATAATAG GTGGGCTGGA TAAGATAGAC 
CTGAATGACT GGAAGTCAAA CACACGCCTG AAGCATTGTG TGGCCGACAG CAACATTGTG 
AGGTGGTTCT GGCAGGCAGT GGAGACCTTC GATGAGGAGA GGAGGGCCAG ACTCCTGCAG 
TTCGTGACCG GGTCCACACG AGTTCCTCTC CAAGGCTTCA AGGCTCTGCA AGGCGCAGCA 
GGGCCTCGAC TCTTCACCAT CCACCTGATA GATGCCAACA CAGACAACTT GCCCAAGGCT 
CATACCTGCT TTAACCGGAT TGACATCCCG CCCTATGAGT CCTATGAGAA GCTCTATGAG 
AAGCTGCTCA CAGCTGTGGA GGAGACCTGT GGCTTTGCTG TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_027413616.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_027269417.1 Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr233610
Clone ID Related Accession (Same CDS sequence)	XM_027413616.1
Accession Version	XM_027413616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-17
Organism	Cricetulus griseus(Chinese hamster)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020822530.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cricetulus griseus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG TAGGAACGGC TCTAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CTGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACTGACACTG TGAAAAACAC TCTGGACCCA 
AAGTGGAACC AGCATTATGA CCTGTACGTT GGGAAAACCG ACTCTATAAC GATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTCGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCGGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCAGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTAGA AAATGAAGGA 
ACAGTGTATG AAGACTCAGG CCCGGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACCGG TGCAGCCGCT GGAGGAGGGA ACTGCAGGTT TGTGGAGTCT 
CCGAGCCAAG ACCAGAGACT CCAGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGTCC 
CTACAGACGC CCCAGAACCG ACCACATGGC CACCAGTCAC CAGAGTTGCC TGAAGGCTAT 
GAGCAAAGGA CGACAGTACA GGGACAAGTT TACTTTTTGC ACACACAGAC TGGAGTCAGC 
ACGTGGCATG ACCCCAGGAT TCCCAGAGAC CTTAATAGTG TGAACTGTGA TGAACTTGGG 
CCGCTGCCTC CAGGCTGGGA AGTCAGAAGT ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAC CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGTTGCCCA GTGAGGGCTC CATGGACGAT 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGA GATTTAGTTC AGAAGCTCAA AGTTCTCAGG 
CACGAACTCT CTCTTCAGCA GCCCCAAGCT GGCCATTGTC GAATAGAAGT TTCCAGAGAA 
GAAATATTTG AGGAGTCATA TCGCCAGATC ATGAAGATGC GGCCCAAGGA CCTGAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGTCTGGATT ATGGCGGAGT GGCACGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AATCCGTATT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACTCT GCAAATCAAC CCAGATTCTT CTGTCAACCC TGACCATCTG 
TCTTACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCATGG GCACTACATA 
AATGGGGGCT TCACAGTCCC ATTCTACAAG CAGCTCCTGG GAAAACCCAT CCAGCTGTCG 
GATCTGGAGT CAGTGGACCC AGAACTGCAC AAGAGTTTGG TGTGGATTCT AGAGAATGAC 
ATCACACCAG TGCTGGACCA TACCTTCTGT GTGGAGCACA ATGCTTTTGG GCGGATCCTT 
CAGCACGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAAAA CAAGAAGGAA 
TATGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TTGAAGCCCA GTTCTTAGCT 
CTTCAGAAAG GGTTTAATGA ACTCATTCCC CAACACTTAC TGAAGCCCTT TGACCAGAAG 
GAACTGGAGC TGATAATAGG TGGGCTGGAT AAGATAGACC TGAATGACTG GAAGTCAAAC 
ACACGCCTGA AGCATTGTGT GGCCGACAGC AACATTGTGA GGTGGTTCTG GCAGGCAGTG 
GAGACCTTCG ATGAGGAGAG GAGGGCCAGA CTCCTGCAGT TCGTGACCGG GTCCACACGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGTTCTACAG GCGCAGCAGG GCCTCGACTC 
TTCACCATCC ACCTGATAGA TGCCAACACA GACAACTTGC CCAAGGCTCA TACCTGCTTT 
AACCGGATTG ACATCCCGCC CTATGAGTCC TATGAGAAGC TCTATGAGAA GCTGCTCACA 
GCTGTGGAGG AGACCTGTGG CTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027269417.1
CDS	216..2411
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGSGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLQVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSMDDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSVNPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_027413616.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027413616.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG TAGGAACGGC TCTAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CTGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACTGACACTG TGAAAAACAC TCTGGACCCA 
AAGTGGAACC AGCATTATGA CCTGTACGTT GGGAAAACCG ACTCTATAAC GATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTCGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCGGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCAGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTAGA AAATGAAGGA 
ACAGTGTATG AAGACTCAGG CCCGGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACCGG TGCAGCCGCT GGAGGAGGGA ACTGCAGGTT TGTGGAGTCT 
CCGAGCCAAG ACCAGAGACT CCAGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGTCC 
CTACAGACGC CCCAGAACCG ACCACATGGC CACCAGTCAC CAGAGTTGCC TGAAGGCTAT 
GAGCAAAGGA CGACAGTACA GGGACAAGTT TACTTTTTGC ACACACAGAC TGGAGTCAGC 
ACGTGGCATG ACCCCAGGAT TCCCAGAGAC CTTAATAGTG TGAACTGTGA TGAACTTGGG 
CCGCTGCCTC CAGGCTGGGA AGTCAGAAGT ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAC CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGTTGCCCA GTGAGGGCTC CATGGACGAT 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGA GATTTAGTTC AGAAGCTCAA AGTTCTCAGG 
CACGAACTCT CTCTTCAGCA GCCCCAAGCT GGCCATTGTC GAATAGAAGT TTCCAGAGAA 
GAAATATTTG AGGAGTCATA TCGCCAGATC ATGAAGATGC GGCCCAAGGA CCTGAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGTCTGGATT ATGGCGGAGT GGCACGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AATCCGTATT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACTCT GCAAATCAAC CCAGATTCTT CTGTCAACCC TGACCATCTG 
TCTTACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCATGG GCACTACATA 
AATGGGGGCT TCACAGTCCC ATTCTACAAG CAGCTCCTGG GAAAACCCAT CCAGCTGTCG 
GATCTGGAGT CAGTGGACCC AGAACTGCAC AAGAGTTTGG TGTGGATTCT AGAGAATGAC 
ATCACACCAG TGCTGGACCA TACCTTCTGT GTGGAGCACA ATGCTTTTGG GCGGATCCTT 
CAGCACGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAAAA CAAGAAGGAA 
TATGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TTGAAGCCCA GTTCTTAGCT 
CTTCAGAAAG GGTTTAATGA ACTCATTCCC CAACACTTAC TGAAGCCCTT TGACCAGAAG 
GAACTGGAGC TGATAATAGG TGGGCTGGAT AAGATAGACC TGAATGACTG GAAGTCAAAC 
ACACGCCTGA AGCATTGTGT GGCCGACAGC AACATTGTGA GGTGGTTCTG GCAGGCAGTG 
GAGACCTTCG ATGAGGAGAG GAGGGCCAGA CTCCTGCAGT TCGTGACCGG GTCCACACGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGTTCTACAG GCGCAGCAGG GCCTCGACTC 
TTCACCATCC ACCTGATAGA TGCCAACACA GACAACTTGC CCAAGGCTCA TACCTGCTTT 
AACCGGATTG ACATCCCGCC CTATGAGTCC TATGAGAAGC TCTATGAGAA GCTGCTCACA 
GCTGTGGAGG AGACCTGTGG CTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_027413617.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_027269418.1 Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCr233611
Clone ID Related Accession (Same CDS sequence)	XM_027413617.1
Accession Version	XM_027413617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-17
Organism	Cricetulus griseus(Chinese hamster)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020822530.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cricetulus griseus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG TAGGAACGGC TCTAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CTGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACTGACACTG TGAAAAACAC TCTGGACCCA 
AAGTGGAACC AGCATTATGA CCTGTACGTT GGGAAAACCG ACTCTATAAC GATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTCGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCGGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCAGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTAGA AAATGAAGGA 
ACAGTGTATG AAGACTCAGG CCCGGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACCGG TGCAGCCGCT GGAGGAGGGA ACTGCAGGTT TGTGGAGTCT 
CCGAGCCAAG ACCAGAGACT CCAGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGTCC 
CTACAGACGC CCCAGAACCG ACCACATGGC CACCAGTCAC CAGAGTTGCC TGAAGGCTAT 
GAGCAAAGGA CGACAGTACA GGGACAAGTT TACTTTTTGC ACACACAGAC TGGAGTCAGC 
ACGTGGCATG ACCCCAGGAT TCCCAGAGAC CTTAATAGTG TGAACTGTGA TGAACTTGGG 
CCGCTGCCTC CAGGCTGGGA AGTCAGAAGT ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAC CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGTTGCCCA GTGAGGGCTC CATGGACGAT 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGA GATTTAGTTC AGAAGCTCAA AGTTCTCAGG 
CACGAACTCT CTCTTCAGCA GCCCCAAGCT GGCCATTGTC GAATAGAAGT TTCCAGAGAA 
GAAATATTTG AGGAGTCATA TCGCCAGATC ATGAAGATGC GGCCCAAGGA CCTGAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGTCTGGATT ATGGCGGAGT GGCACGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AATCCGTATT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACTCT GCAAATCAAC CCAGATTCTT CTGTCAACCC TGACCATCTG 
TCTTACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCATGG GCACTACATA 
AATGGGGGCT TCACAGTCCC ATTCTACAAG CAGCTCCTGG GAAAACCCAT CCAGCTGTCG 
GATCTGGAGT CAGTGGACCC AGAACTGCAC AAGAGTTTGG TGTGGATTCT AGAGAATGAC 
ATCACACCAG TGCTGGACCA TACCTTCTGT GTGGAGCACA ATGCTTTTGG GCGGATCCTT 
CAGCACGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAAAA CAAGAAGGAA 
TATGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TTGAAGCCCA GTTCTTAGCT 
CTTCAGAAAG GGTTTAATGA ACTCATTCCC CAACACTTAC TGAAGCCCTT TGACCAGAAG 
GAACTGGAGC TGATAATAGG TGGGCTGGAT AAGATAGACC TGAATGACTG GAAGTCAAAC 
ACACGCCTGA AGCATTGTGT GGCCGACAGC AACATTGTGA GGTGGTTCTG GCAGGCAGTG 
GAGACCTTCG ATGAGGAGAG GAGGGCCAGA CTCCTGCAGT TCGTGACCGG GTCCACACGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCGCAGCAG GGCCTCGACT CTTCACCATC 
CACCTGATAG ATGCCAACAC AGACAACTTG CCCAAGGCTC ATACCTGCTT TAACCGGATT 
GACATCCCGC CCTATGAGTC CTATGAGAAG CTCTATGAGA AGCTGCTCAC AGCTGTGGAG 
GAGACCTGTG GCTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027269418.1
CDS	217..2403
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGSGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLQVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSMDDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSVNPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_027413617.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027413617.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG TAGGAACGGC TCTAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CTGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACTGACACTG TGAAAAACAC TCTGGACCCA 
AAGTGGAACC AGCATTATGA CCTGTACGTT GGGAAAACCG ACTCTATAAC GATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTCGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCGGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCAGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTAGA AAATGAAGGA 
ACAGTGTATG AAGACTCAGG CCCGGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACCGG TGCAGCCGCT GGAGGAGGGA ACTGCAGGTT TGTGGAGTCT 
CCGAGCCAAG ACCAGAGACT CCAGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGTCC 
CTACAGACGC CCCAGAACCG ACCACATGGC CACCAGTCAC CAGAGTTGCC TGAAGGCTAT 
GAGCAAAGGA CGACAGTACA GGGACAAGTT TACTTTTTGC ACACACAGAC TGGAGTCAGC 
ACGTGGCATG ACCCCAGGAT TCCCAGAGAC CTTAATAGTG TGAACTGTGA TGAACTTGGG 
CCGCTGCCTC CAGGCTGGGA AGTCAGAAGT ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAC CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGTTGCCCA GTGAGGGCTC CATGGACGAT 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGA GATTTAGTTC AGAAGCTCAA AGTTCTCAGG 
CACGAACTCT CTCTTCAGCA GCCCCAAGCT GGCCATTGTC GAATAGAAGT TTCCAGAGAA 
GAAATATTTG AGGAGTCATA TCGCCAGATC ATGAAGATGC GGCCCAAGGA CCTGAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGTCTGGATT ATGGCGGAGT GGCACGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AATCCGTATT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACTCT GCAAATCAAC CCAGATTCTT CTGTCAACCC TGACCATCTG 
TCTTACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCATGG GCACTACATA 
AATGGGGGCT TCACAGTCCC ATTCTACAAG CAGCTCCTGG GAAAACCCAT CCAGCTGTCG 
GATCTGGAGT CAGTGGACCC AGAACTGCAC AAGAGTTTGG TGTGGATTCT AGAGAATGAC 
ATCACACCAG TGCTGGACCA TACCTTCTGT GTGGAGCACA ATGCTTTTGG GCGGATCCTT 
CAGCACGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAAAA CAAGAAGGAA 
TATGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TTGAAGCCCA GTTCTTAGCT 
CTTCAGAAAG GGTTTAATGA ACTCATTCCC CAACACTTAC TGAAGCCCTT TGACCAGAAG 
GAACTGGAGC TGATAATAGG TGGGCTGGAT AAGATAGACC TGAATGACTG GAAGTCAAAC 
ACACGCCTGA AGCATTGTGT GGCCGACAGC AACATTGTGA GGTGGTTCTG GCAGGCAGTG 
GAGACCTTCG ATGAGGAGAG GAGGGCCAGA CTCCTGCAGT TCGTGACCGG GTCCACACGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCGCAGCAG GGCCTCGACT CTTCACCATC 
CACCTGATAG ATGCCAACAC AGACAACTTG CCCAAGGCTC ATACCTGCTT TAACCGGATT 
GACATCCCGC CCTATGAGTC CTATGAGAAG CTCTATGAGA AGCTGCTCAC AGCTGTGGAG 
GAGACCTGTG GCTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_027413618.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_027269419.1 Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCr233612
Clone ID Related Accession (Same CDS sequence)	XM_027413618.1
Accession Version	XM_027413618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-17
Organism	Cricetulus griseus(Chinese hamster)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020822530.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cricetulus griseus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG TAGGAACGGC TCTAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CTGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACTGACACTG TGAAAAACAC TCTGGACCCA 
AAGTGGAACC AGCATTATGA CCTGTACGTT GGGAAAACCG ACTCTATAAC GATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTCGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTTCA GTTTACAGAC CCGAGACAGA 
ATAGGCAGTG GAGGGTCGGT GGTGGACTGC AGGGGACTGC TAGAAAATGA AGGAACAGTG 
TATGAAGACT CAGGCCCGGG AAGGCCGCTC AGCTGCCTCA TGGAGGAACC TGCCCCATAT 
ACAGATGGTA CCGGTGCAGC CGCTGGAGGA GGGAACTGCA GGTTTGTGGA GTCTCCGAGC 
CAAGACCAGA GACTCCAGGT ACAGCGACTC CGAAATCCTG AGGTTCGAGG GTCCCTACAG 
ACGCCCCAGA ACCGACCACA TGGCCACCAG TCACCAGAGT TGCCTGAAGG CTATGAGCAA 
AGGACGACAG TACAGGGACA AGTTTACTTT TTGCACACAC AGACTGGAGT CAGCACGTGG 
CATGACCCCA GGATTCCCAG AGACCTTAAT AGTGTGAACT GTGATGAACT TGGGCCGCTG 
CCTCCAGGCT GGGAAGTCAG AAGTACAGTG TCGGGAAGAA TCTATTTTGT AGATCACAAT 
AATAGGACAA CCCAGTTTAC AGACCCACGG CTTCACCACA TCATGAATCA CCAGTGCCAA 
CTCAAGGAGC CCAGCCAGCC GCTGCAGTTG CCCAGTGAGG GCTCCATGGA CGATGAGGAG 
CTTCCTGCCC AGAGATATGA GAGAGATTTA GTTCAGAAGC TCAAAGTTCT CAGGCACGAA 
CTCTCTCTTC AGCAGCCCCA AGCTGGCCAT TGTCGAATAG AAGTTTCCAG AGAAGAAATA 
TTTGAGGAGT CATATCGCCA GATCATGAAG ATGCGGCCCA AGGACCTGAA GAAGCGCCTG 
ATGGTGAAGT TCCGAGGGGA GGAAGGTCTG GATTATGGCG GAGTGGCACG GGAGTGGCTG 
TATTTGTTGT GCCATGAAAT GTTGAATCCG TATTATGGAC TCTTCCAGTA TTCCACGGAC 
AATATTTACA CTCTGCAAAT CAACCCAGAT TCTTCTGTCA ACCCTGACCA TCTGTCTTAC 
TTCCACTTTG TGGGTCGCAT CATGGGTCTG GCTGTGTTCC ATGGGCACTA CATAAATGGG 
GGCTTCACAG TCCCATTCTA CAAGCAGCTC CTGGGAAAAC CCATCCAGCT GTCGGATCTG 
GAGTCAGTGG ACCCAGAACT GCACAAGAGT TTGGTGTGGA TTCTAGAGAA TGACATCACA 
CCAGTGCTGG ACCATACCTT CTGTGTGGAG CACAATGCTT TTGGGCGGAT CCTTCAGCAC 
GAACTGAAAC CCAATGGCAG AAATGTGCCT GTCACTGAGG AAAACAAGAA GGAATATGTC 
CGGTTGTATG TAAACTGGAG GTTTATGAGA GGAATTGAAG CCCAGTTCTT AGCTCTTCAG 
AAAGGGTTTA ATGAACTCAT TCCCCAACAC TTACTGAAGC CCTTTGACCA GAAGGAACTG 
GAGCTGATAA TAGGTGGGCT GGATAAGATA GACCTGAATG ACTGGAAGTC AAACACACGC 
CTGAAGCATT GTGTGGCCGA CAGCAACATT GTGAGGTGGT TCTGGCAGGC AGTGGAGACC 
TTCGATGAGG AGAGGAGGGC CAGACTCCTG CAGTTCGTGA CCGGGTCCAC ACGAGTTCCT 
CTCCAAGGCT TCAAGGCTCT GCAAGGTTCT ACAGGCGCAG CAGGGCCTCG ACTCTTCACC 
ATCCACCTGA TAGATGCCAA CACAGACAAC TTGCCCAAGG CTCATACCTG CTTTAACCGG 
ATTGACATCC CGCCCTATGA GTCCTATGAG AAGCTCTATG AGAAGCTGCT CACAGCTGTG 
GAGGAGACCT GTGGCTTTGC TGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027269419.1
CDS	217..2346
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGFSLQTRDRIGSGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLQVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSMDDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSVNPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_027413618.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027413618.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG TAGGAACGGC TCTAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CTGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACTGACACTG TGAAAAACAC TCTGGACCCA 
AAGTGGAACC AGCATTATGA CCTGTACGTT GGGAAAACCG ACTCTATAAC GATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTCGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTTCA GTTTACAGAC CCGAGACAGA 
ATAGGCAGTG GAGGGTCGGT GGTGGACTGC AGGGGACTGC TAGAAAATGA AGGAACAGTG 
TATGAAGACT CAGGCCCGGG AAGGCCGCTC AGCTGCCTCA TGGAGGAACC TGCCCCATAT 
ACAGATGGTA CCGGTGCAGC CGCTGGAGGA GGGAACTGCA GGTTTGTGGA GTCTCCGAGC 
CAAGACCAGA GACTCCAGGT ACAGCGACTC CGAAATCCTG AGGTTCGAGG GTCCCTACAG 
ACGCCCCAGA ACCGACCACA TGGCCACCAG TCACCAGAGT TGCCTGAAGG CTATGAGCAA 
AGGACGACAG TACAGGGACA AGTTTACTTT TTGCACACAC AGACTGGAGT CAGCACGTGG 
CATGACCCCA GGATTCCCAG AGACCTTAAT AGTGTGAACT GTGATGAACT TGGGCCGCTG 
CCTCCAGGCT GGGAAGTCAG AAGTACAGTG TCGGGAAGAA TCTATTTTGT AGATCACAAT 
AATAGGACAA CCCAGTTTAC AGACCCACGG CTTCACCACA TCATGAATCA CCAGTGCCAA 
CTCAAGGAGC CCAGCCAGCC GCTGCAGTTG CCCAGTGAGG GCTCCATGGA CGATGAGGAG 
CTTCCTGCCC AGAGATATGA GAGAGATTTA GTTCAGAAGC TCAAAGTTCT CAGGCACGAA 
CTCTCTCTTC AGCAGCCCCA AGCTGGCCAT TGTCGAATAG AAGTTTCCAG AGAAGAAATA 
TTTGAGGAGT CATATCGCCA GATCATGAAG ATGCGGCCCA AGGACCTGAA GAAGCGCCTG 
ATGGTGAAGT TCCGAGGGGA GGAAGGTCTG GATTATGGCG GAGTGGCACG GGAGTGGCTG 
TATTTGTTGT GCCATGAAAT GTTGAATCCG TATTATGGAC TCTTCCAGTA TTCCACGGAC 
AATATTTACA CTCTGCAAAT CAACCCAGAT TCTTCTGTCA ACCCTGACCA TCTGTCTTAC 
TTCCACTTTG TGGGTCGCAT CATGGGTCTG GCTGTGTTCC ATGGGCACTA CATAAATGGG 
GGCTTCACAG TCCCATTCTA CAAGCAGCTC CTGGGAAAAC CCATCCAGCT GTCGGATCTG 
GAGTCAGTGG ACCCAGAACT GCACAAGAGT TTGGTGTGGA TTCTAGAGAA TGACATCACA 
CCAGTGCTGG ACCATACCTT CTGTGTGGAG CACAATGCTT TTGGGCGGAT CCTTCAGCAC 
GAACTGAAAC CCAATGGCAG AAATGTGCCT GTCACTGAGG AAAACAAGAA GGAATATGTC 
CGGTTGTATG TAAACTGGAG GTTTATGAGA GGAATTGAAG CCCAGTTCTT AGCTCTTCAG 
AAAGGGTTTA ATGAACTCAT TCCCCAACAC TTACTGAAGC CCTTTGACCA GAAGGAACTG 
GAGCTGATAA TAGGTGGGCT GGATAAGATA GACCTGAATG ACTGGAAGTC AAACACACGC 
CTGAAGCATT GTGTGGCCGA CAGCAACATT GTGAGGTGGT TCTGGCAGGC AGTGGAGACC 
TTCGATGAGG AGAGGAGGGC CAGACTCCTG CAGTTCGTGA CCGGGTCCAC ACGAGTTCCT 
CTCCAAGGCT TCAAGGCTCT GCAAGGTTCT ACAGGCGCAG CAGGGCCTCG ACTCTTCACC 
ATCCACCTGA TAGATGCCAA CACAGACAAC TTGCCCAAGG CTCATACCTG CTTTAACCGG 
ATTGACATCC CGCCCTATGA GTCCTATGAG AAGCTCTATG AGAAGCTGCT CACAGCTGTG 
GAGGAGACCT GTGGCTTTGC TGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_016978516.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_016834005.1 Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr24268
Clone ID Related Accession (Same CDS sequence)	XM_016978516.1
Accession Version	XM_016978516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-26
Organism	Cricetulus griseus(Chinese hamster)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003614494.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cricetulus griseus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAACAAA CTGCAGAGTG TTTCTTCTCC CCATCCTCCC CTGGCCAGTC GACACTGGGC 
ACAGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGACTTCT TCAGACTCCC TGACCCCTTT 
GCCAAGATTG TTGTGGACGG CTCTGGGCAG TGCCACTCAA CTGACACTGT GAAAAACACT 
CTGGACCCAA AGTGGAACCA GCATTATGAC CTGTACGTTG GGAAAACCGA CTCTATAACG 
ATCAGTGTGT GGAACCACAA GAAGATCCAC AAGAAGCAGG GGGCTGGCTT CCTCGGCTGC 
GTGCGGCTGC TCTCCAATGC CATCAGCAGA TTGAAAGACA CTGGCTACCA GCGTTTGGAT 
CTATGCAAAC TAAATCCCTC AGATACTGAT GCGGTTCGTG GCCAAATAGT GGTCAGTTTA 
CAGACCCGAG ACAGAATAGG CAGTGGAGGG TCGGTGGTGG ACTGCAGGGG ACTGCTAGAA 
AATGAAGGAA CAGTGTATGA AGACTCAGGC CCGGGAAGGC CGCTCAGCTG CCTCATGGAG 
GAACCTGCCC CATATACAGA TGGTACCGGT GCAGCCGCTG GAGGAGGGAA CTGCAGGTTT 
GTGGAGTCTC CGAGCCAAGA CCAGAGACTC CAGGTACAGC GACTCCGAAA TCCTGAGGTT 
CGAGGGTCCC TACAGACGCC CCAGAACCGA CCACATGGCC ACCAGTCACC AGAGTTGCCT 
GAAGGCTATG AGCAAAGGAC GACAGTACAG GGACAAGTTT ACTTTTTGCA CACACAGACT 
GGAGTCAGCA CGTGGCATGA CCCCAGGATT CCCAGAGACC TTAATAGTGT GAACTGTGAT 
GAACTTGGGC CGCTGCCTCC AGGCTGGGAA GTCAGAAGTA CAGTGTCGGG AAGAATCTAT 
TTTGTAGATC ACAATAATAG GACAACCCAG TTTACAGACC CACGGCTTCA CCACATCATG 
AATCACCAGT GCCAACTCAA GGAGCCCAGC CAGCCGCTGC AGTTGCCCAG TGAGGGCTCC 
ATGGACGATG AGGAGCTTCC TGCCCAGAGA TATGAGAGAG ATTTAGTTCA GAAGCTCAAA 
GTTCTCAGGC ACGAACTCTC TCTTCAGCAG CCCCAAGCTG GCCATTGTCG AATAGAAGTT 
TCCAGAGAAG AAATATTTGA GGAGTCATAT CGCCAGATCA TGAAGATGCG GCCCAAGGAC 
CTGAAGAAGC GCCTGATGGT GAAGTTCCGA GGGGAGGAAG GTCTGGATTA TGGCGGAGTG 
GCACGGGAGT GGCTGTATTT GTTGTGCCAT GAAATGTTGA ATCCGTATTA TGGACTCTTC 
CAGTATTCCA CGGACAATAT TTACACTCTG CAAATCAACC CAGATTCTTC TGTCAACCCT 
GACCATCTGT CTTACTTCCA CTTTGTGGGT CGCATCATGG GTCTGGCTGT GTTCCATGGG 
CACTACATAA ATGGGGGCTT CACAGTCCCA TTCTACAAGC AGCTCCTGGG AAAACCCATC 
CAGCTGTCGG ATCTGGAGTC AGTGGACCCA GAACTGCACA AGAGTTTGGT GTGGATTCTA 
GAGAATGACA TCACACCAGT GCTGGACCAT ACCTTCTGTG TGGAGCACAA TGCTTTTGGG 
CGGATCCTTC AGCACGAACT GAAACCCAAT GGCAGAAATG TGCCTGTCAC TGAGGAAAAC 
AAGAAGGAAT ATGTCCGGTT GTATGTAAAC TGGAGGTTTA TGAGAGGAAT TGAAGCCCAG 
TTCTTAGCTC TTCAGAAAGG GTTTAATGAA CTCATTCCCC AACACTTACT GAAGCCCTTT 
GACCAGAAGG AACTGGAGCT GATAATAGGT GGGCTGGATA AGATAGACCT GAATGACTGG 
AAGTCAAACA CACGCCTGAA GCATTGTGTG GCCGACAGCA ACATTGTGAG GTGGTTCTGG 
CAGGCAGTGG AGACCTTCGA TGAGGAGAGG AGGGCCAGAC TCCTGCAGTT CGTGACCGGG 
TCCACACGAG TTCCTCTCCA AGGCTTCAAG GCTCTGCAAG GTTCTACAGG CGCAGCAGGG 
CCTCGACTCT TCACCATCCA CCTGATAGAT GCCAACACAG ACAACTTGCC CAAGGCTCAT 
ACCTGCTTTA ACCGGATTGA CATCCCGCCC TATGAGTCCT ATGAGAAGCT CTATGAGAAG 
CTGCTCACAG CTGTGGAGGA GACCTGTGGC TTTGCTGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016834005.1
CDS	1..2205
Translation	MEQTAECFFSPSSPGQSTLGTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGSGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLQVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSMDDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSVNPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_016978516.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016978516.1

ORF Insert Sequence:

ATGGAACAAA CTGCAGAGTG TTTCTTCTCC CCATCCTCCC CTGGCCAGTC GACACTGGGC 
ACAGTATTAT GTGCCAAGAA CCTTGCAAAG AAAGACTTCT TCAGACTCCC TGACCCCTTT 
GCCAAGATTG TTGTGGACGG CTCTGGGCAG TGCCACTCAA CTGACACTGT GAAAAACACT 
CTGGACCCAA AGTGGAACCA GCATTATGAC CTGTACGTTG GGAAAACCGA CTCTATAACG 
ATCAGTGTGT GGAACCACAA GAAGATCCAC AAGAAGCAGG GGGCTGGCTT CCTCGGCTGC 
GTGCGGCTGC TCTCCAATGC CATCAGCAGA TTGAAAGACA CTGGCTACCA GCGTTTGGAT 
CTATGCAAAC TAAATCCCTC AGATACTGAT GCGGTTCGTG GCCAAATAGT GGTCAGTTTA 
CAGACCCGAG ACAGAATAGG CAGTGGAGGG TCGGTGGTGG ACTGCAGGGG ACTGCTAGAA 
AATGAAGGAA CAGTGTATGA AGACTCAGGC CCGGGAAGGC CGCTCAGCTG CCTCATGGAG 
GAACCTGCCC CATATACAGA TGGTACCGGT GCAGCCGCTG GAGGAGGGAA CTGCAGGTTT 
GTGGAGTCTC CGAGCCAAGA CCAGAGACTC CAGGTACAGC GACTCCGAAA TCCTGAGGTT 
CGAGGGTCCC TACAGACGCC CCAGAACCGA CCACATGGCC ACCAGTCACC AGAGTTGCCT 
GAAGGCTATG AGCAAAGGAC GACAGTACAG GGACAAGTTT ACTTTTTGCA CACACAGACT 
GGAGTCAGCA CGTGGCATGA CCCCAGGATT CCCAGAGACC TTAATAGTGT GAACTGTGAT 
GAACTTGGGC CGCTGCCTCC AGGCTGGGAA GTCAGAAGTA CAGTGTCGGG AAGAATCTAT 
TTTGTAGATC ACAATAATAG GACAACCCAG TTTACAGACC CACGGCTTCA CCACATCATG 
AATCACCAGT GCCAACTCAA GGAGCCCAGC CAGCCGCTGC AGTTGCCCAG TGAGGGCTCC 
ATGGACGATG AGGAGCTTCC TGCCCAGAGA TATGAGAGAG ATTTAGTTCA GAAGCTCAAA 
GTTCTCAGGC ACGAACTCTC TCTTCAGCAG CCCCAAGCTG GCCATTGTCG AATAGAAGTT 
TCCAGAGAAG AAATATTTGA GGAGTCATAT CGCCAGATCA TGAAGATGCG GCCCAAGGAC 
CTGAAGAAGC GCCTGATGGT GAAGTTCCGA GGGGAGGAAG GTCTGGATTA TGGCGGAGTG 
GCACGGGAGT GGCTGTATTT GTTGTGCCAT GAAATGTTGA ATCCGTATTA TGGACTCTTC 
CAGTATTCCA CGGACAATAT TTACACTCTG CAAATCAACC CAGATTCTTC TGTCAACCCT 
GACCATCTGT CTTACTTCCA CTTTGTGGGT CGCATCATGG GTCTGGCTGT GTTCCATGGG 
CACTACATAA ATGGGGGCTT CACAGTCCCA TTCTACAAGC AGCTCCTGGG AAAACCCATC 
CAGCTGTCGG ATCTGGAGTC AGTGGACCCA GAACTGCACA AGAGTTTGGT GTGGATTCTA 
GAGAATGACA TCACACCAGT GCTGGACCAT ACCTTCTGTG TGGAGCACAA TGCTTTTGGG 
CGGATCCTTC AGCACGAACT GAAACCCAAT GGCAGAAATG TGCCTGTCAC TGAGGAAAAC 
AAGAAGGAAT ATGTCCGGTT GTATGTAAAC TGGAGGTTTA TGAGAGGAAT TGAAGCCCAG 
TTCTTAGCTC TTCAGAAAGG GTTTAATGAA CTCATTCCCC AACACTTACT GAAGCCCTTT 
GACCAGAAGG AACTGGAGCT GATAATAGGT GGGCTGGATA AGATAGACCT GAATGACTGG 
AAGTCAAACA CACGCCTGAA GCATTGTGTG GCCGACAGCA ACATTGTGAG GTGGTTCTGG 
CAGGCAGTGG AGACCTTCGA TGAGGAGAGG AGGGCCAGAC TCCTGCAGTT CGTGACCGGG 
TCCACACGAG TTCCTCTCCA AGGCTTCAAG GCTCTGCAAG GTTCTACAGG CGCAGCAGGG 
CCTCGACTCT TCACCATCCA CCTGATAGAT GCCAACACAG ACAACTTGCC CAAGGCTCAT 
ACCTGCTTTA ACCGGATTGA CATCCCGCCC TATGAGTCCT ATGAGAAGCT CTATGAGAAG 
CTGCTCACAG CTGTGGAGGA GACCTGTGGC TTTGCTGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_016966968.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_016822457.1 Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

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CloneID	OCr38728
Clone ID Related Accession (Same CDS sequence)	XM_016966968.1
Accession Version	XM_016966968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-26
Organism	Cricetulus griseus(Chinese hamster)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006874671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cricetulus griseus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGAAGACA GTGGTGCTGA GAGCAGTGTA GATATGATGG CCCAGCTCAA GAGGTTTCAG 
ATGAAAAGCA TCAAGTGTTT CTTCTCCCCA TCCTCCCCTG GCCAGTCGAC ACTGGGCACA 
GTATTATGTG CCAAGAACCT TGCAAAGAAA GACTTCTTCA GACTCCCTGA CCCCTTTGCC 
AAGATTGTTG TGGACGGCTC TGGGCAGTGC CACTCAACTG ACACTGTGAA AAACACTCTG 
GACCCAAAGT GGAACCAGCA TTATGACCTG TACGTTGGGA AAACCGACTC TATAACGATC 
AGTGTGTGGA ACCACAAGAA GATCCACAAG AAGCAGGGGG CTGGCTTCCT CGGCTGCGTG 
CGGCTGCTCT CCAATGCCAT CAGCAGATTG AAAGACACTG GCTACCAGCG TTTGGATCTA 
TGCAAACTAA ATCCCTCAGA TACTGATGCG GTTCGTGGCC AAATAGTGGT CAGTTTACAG 
ACCCGAGACA GAATAGGCAG TGGAGGGTCG GTGGTGGACT GCAGGGGACT GCTAGAAAAT 
GAAGGAACAG TGTATGAAGA CTCAGGCCCG GGAAGGCCGC TCAGCTGCCT CATGGAGGAA 
CCTGCCCCAT ATACAGATGG TACCGGTGCA GCCGCTGGAG GAGGGAACTG CAGGTTTGTG 
GAGTCTCCGA GCCAAGACCA GAGACTCCAG GTACAGCGAC TCCGAAATCC TGAGGTTCGA 
GGGTCCCTAC AGACGCCCCA GAACCGACCA CATGGCCACC AGTCACCAGA GTTGCCTGAA 
GGCTATGAGC AAAGGACGAC AGTACAGGGA CAAGTTTACT TTTTGCACAC ACAGACTGGA 
GTCAGCACGT GGCATGACCC CAGGATTCCC AGAGACCTTA ATAGTGTGAA CTGTGATGAA 
CTTGGGCCGC TGCCTCCAGG CTGGGAAGTC AGAAGTACAG TGTCGGGAAG AATCTATTTT 
GTAGATCACA ATAATAGGAC AACCCAGTTT ACAGACCCAC GGCTTCACCA CATCATGAAT 
CACCAGTGCC AACTCAAGGA GCCCAGCCAG CCGCTGCAGT TGCCCAGTGA GGGCTCCATG 
GACGATGAGG AGCTTCCTGC CCAGAGATAT GAGAGAGATT TAGTTCAGAA GCTCAAAGTT 
CTCAGGCACG AACTCTCTCT TCAGCAGCCC CAAGCTGGCC ATTGTCGAAT AGAAGTTTCC 
AGAGAAGAAA TATTTGAGGA GTCATATCGC CAGATCATGA AGATGCGGCC CAAGGACCTG 
AAGAAGCGCC TGATGGTGAA GTTCCGAGGG GAGGAAGGTC TGGATTATGG CGGAGTGGCA 
CGGGAGTGGC TGTATTTGTT GTGCCATGAA ATGTTGAATC CGTATTATGG ACTCTTCCAG 
TATTCCACGG ACAATATTTA CACTCTGCAA ATCAACCCAG ATTCTTCTGT CAACCCTGAC 
CATCTGTCTT ACTTCCACTT TGTGGGTCGC ATCATGGGTC TGGCTGTGTT CCATGGGCAC 
TACATAAATG GGGGCTTCAC AGTCCCATTC TACAAGCAGC TCCTGGGAAA ACCCATCCAG 
CTGTCGGATC TGGAGTCAGT GGACCCAGAA CTGCACAAGA GTTTGGTGTG GATTCTAGAG 
AATGACATCA CACCAGTGCT GGACCATACC TTCTGTGTGG AGCACAATGC TTTTGGGCGG 
ATCCTTCAGC ACGAACTGAA ACCCAATGGC AGAAATGTGC CTGTCACTGA GGAAAACAAG 
AAGGAATATG TCCGGTTGTA TGTAAACTGG AGGTTTATGA GAGGAATTGA AGCCCAGTTC 
TTAGCTCTTC AGAAAGGGTT TAATGAACTC ATTCCCCAAC ACTTACTGAA GCCCTTTGAC 
CAGAAGGAAC TGGAGCTGAT AATAGGTGGG CTGGATAAGA TAGACCTGAA TGACTGGAAG 
TCAAACACAC GCCTGAAGCA TTGTGTGGCC GACAGCAACA TTGTGAGGTG GTTCTGGCAG 
GCAGTGGAGA CCTTCGATGA GGAGAGGAGG GCCAGACTCC TGCAGTTCGT GACCGGGTCC 
ACACGAGTTC CTCTCCAAGG CTTCAAGGCT CTGCAAGGTT CTACAGGCGC AGCAGGGCCT 
CGACTCTTCA CCATCCACCT GATAGATGCC AACACAGACA ACTTGCCCAA GGCTCATACC 
TGCTTTAACC GGATTGACAT CCCGCCCTAT GAGTCCTATG AGAAGCTCTA TGAGAAGCTG 
CTCACAGCTG TGGAGGAGAC CTGTGGCTTT GCTGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016822457.1
CDS	1..2262
Translation	MEDSGAESSVDMMAQLKRFQMKSIKCFFSPSSPGQSTLGTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGSGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLQVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSMDDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSVNPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_016966968.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016966968.1

ORF Insert Sequence:

ATGGAAGACA GTGGTGCTGA GAGCAGTGTA GATATGATGG CCCAGCTCAA GAGGTTTCAG 
ATGAAAAGCA TCAAGTGTTT CTTCTCCCCA TCCTCCCCTG GCCAGTCGAC ACTGGGCACA 
GTATTATGTG CCAAGAACCT TGCAAAGAAA GACTTCTTCA GACTCCCTGA CCCCTTTGCC 
AAGATTGTTG TGGACGGCTC TGGGCAGTGC CACTCAACTG ACACTGTGAA AAACACTCTG 
GACCCAAAGT GGAACCAGCA TTATGACCTG TACGTTGGGA AAACCGACTC TATAACGATC 
AGTGTGTGGA ACCACAAGAA GATCCACAAG AAGCAGGGGG CTGGCTTCCT CGGCTGCGTG 
CGGCTGCTCT CCAATGCCAT CAGCAGATTG AAAGACACTG GCTACCAGCG TTTGGATCTA 
TGCAAACTAA ATCCCTCAGA TACTGATGCG GTTCGTGGCC AAATAGTGGT CAGTTTACAG 
ACCCGAGACA GAATAGGCAG TGGAGGGTCG GTGGTGGACT GCAGGGGACT GCTAGAAAAT 
GAAGGAACAG TGTATGAAGA CTCAGGCCCG GGAAGGCCGC TCAGCTGCCT CATGGAGGAA 
CCTGCCCCAT ATACAGATGG TACCGGTGCA GCCGCTGGAG GAGGGAACTG CAGGTTTGTG 
GAGTCTCCGA GCCAAGACCA GAGACTCCAG GTACAGCGAC TCCGAAATCC TGAGGTTCGA 
GGGTCCCTAC AGACGCCCCA GAACCGACCA CATGGCCACC AGTCACCAGA GTTGCCTGAA 
GGCTATGAGC AAAGGACGAC AGTACAGGGA CAAGTTTACT TTTTGCACAC ACAGACTGGA 
GTCAGCACGT GGCATGACCC CAGGATTCCC AGAGACCTTA ATAGTGTGAA CTGTGATGAA 
CTTGGGCCGC TGCCTCCAGG CTGGGAAGTC AGAAGTACAG TGTCGGGAAG AATCTATTTT 
GTAGATCACA ATAATAGGAC AACCCAGTTT ACAGACCCAC GGCTTCACCA CATCATGAAT 
CACCAGTGCC AACTCAAGGA GCCCAGCCAG CCGCTGCAGT TGCCCAGTGA GGGCTCCATG 
GACGATGAGG AGCTTCCTGC CCAGAGATAT GAGAGAGATT TAGTTCAGAA GCTCAAAGTT 
CTCAGGCACG AACTCTCTCT TCAGCAGCCC CAAGCTGGCC ATTGTCGAAT AGAAGTTTCC 
AGAGAAGAAA TATTTGAGGA GTCATATCGC CAGATCATGA AGATGCGGCC CAAGGACCTG 
AAGAAGCGCC TGATGGTGAA GTTCCGAGGG GAGGAAGGTC TGGATTATGG CGGAGTGGCA 
CGGGAGTGGC TGTATTTGTT GTGCCATGAA ATGTTGAATC CGTATTATGG ACTCTTCCAG 
TATTCCACGG ACAATATTTA CACTCTGCAA ATCAACCCAG ATTCTTCTGT CAACCCTGAC 
CATCTGTCTT ACTTCCACTT TGTGGGTCGC ATCATGGGTC TGGCTGTGTT CCATGGGCAC 
TACATAAATG GGGGCTTCAC AGTCCCATTC TACAAGCAGC TCCTGGGAAA ACCCATCCAG 
CTGTCGGATC TGGAGTCAGT GGACCCAGAA CTGCACAAGA GTTTGGTGTG GATTCTAGAG 
AATGACATCA CACCAGTGCT GGACCATACC TTCTGTGTGG AGCACAATGC TTTTGGGCGG 
ATCCTTCAGC ACGAACTGAA ACCCAATGGC AGAAATGTGC CTGTCACTGA GGAAAACAAG 
AAGGAATATG TCCGGTTGTA TGTAAACTGG AGGTTTATGA GAGGAATTGA AGCCCAGTTC 
TTAGCTCTTC AGAAAGGGTT TAATGAACTC ATTCCCCAAC ACTTACTGAA GCCCTTTGAC 
CAGAAGGAAC TGGAGCTGAT AATAGGTGGG CTGGATAAGA TAGACCTGAA TGACTGGAAG 
TCAAACACAC GCCTGAAGCA TTGTGTGGCC GACAGCAACA TTGTGAGGTG GTTCTGGCAG 
GCAGTGGAGA CCTTCGATGA GGAGAGGAGG GCCAGACTCC TGCAGTTCGT GACCGGGTCC 
ACACGAGTTC CTCTCCAAGG CTTCAAGGCT CTGCAAGGTT CTACAGGCGC AGCAGGGCCT 
CGACTCTTCA CCATCCACCT GATAGATGCC AACACAGACA ACTTGCCCAA GGCTCATACC 
TGCTTTAACC GGATTGACAT CCCGCCCTAT GAGTCCTATG AGAAGCTCTA TGAGAAGCTG 
CTCACAGCTG TGGAGGAGAC CTGTGGCTTT GCTGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Smurf1 cDNA ORF clone, Cricetulus griseus(Chinese hamster)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム