LOC105345258 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105345258
Entrez Gene ID	105345258
Full Name	ubiquitin-conjugating enzyme E2 E1
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020074114.1	XP_019929673.1	ubiquitin-conjugating enzyme E2 E1

Pathways from BioSystems
Gene Ontology

The following LOC105345258 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105345258 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr112387	XM_020074114.1 Latest version!	Crassostrea gigas ubiquitin-conjugating enzyme E2 E1 (LOC105345258), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105345258 ( XM_020074114.1 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_019929673.1 Crassostrea gigas ubiquitin-conjugating enzyme E2 E1 (LOC105345258), mRNA.

Price & Availability↑

CloneID	OCr112387
Clone ID Related Accession (Same CDS sequence)	XM_020074114.1
Accession Version	XM_020074114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	ubiquitin-conjugating enzyme E2 E1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011937001.1) and transcript sequence (GECI01036291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 54 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAAAAGA AGTATAGGAT CTACATGACA GTAACAGATA GTGACTCTGC CTGTTCGGGA 
ATGTCTTCAG ATCGGGGAAG CGGTAGGGGT AGGGGCAGAC GATCTCGAGG GGCACAGAAT 
AACGAAGGAA CCCACAGTTC AGGTCCCGAC AGCCCAGCCG AGGCGAACGG CCCCAGCACA 
TCGGCGGTTC AGGCCGAGCC CCCCGCCCCT GCCCCAAAAA CATCCAACAA AAGCTTCAAA 
CCCACGGGCA CCAGTCTAAA ACGAATTCAA AAAGAATTGG CAGAAATCAC CATTGACCCT 
CCGCCGAATT GCAGTGCTGG TCCCAAGGGG GATAACATGT ATGAATGGGT GTCCACCATT 
CTGGGACCCC CAGGGTCTGT GTACGAGGGA GGCGTATTCT TCTTGGACAT TCATTTCTCA 
GCGGATTATC CGTTTAAGCC ACCAAAGGTG ACATTTCGAA CACGGATTTA CCATTGCAAT 
ATAAACAGCC AGGGTGTGAT CTGTCTGGAC ATTCTAAAGG ACAATTGGAG TCCTGCTCTG 
ACTATCTCCA AAGTGTTGTT GTCCATTTGT TCCCTTCTAA CAGACTGTAA TCCGGCCGAC 
CCTCTGGTTG GGAGCATCGC TACTCAGTAC ACTCAGAACA GGACGGAACA CGACAGGGTG 
GCTCGCCAGT GGACCAAGAG GTTCGCCACG GGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019929673.1
CDS	1..699
Translation	MKKKYRIYMTVTDSDSACSGMSSDRGSGRGRGRRSRGAQNNEGTHSSGPDSPAEANGPSTSAVQAEPPAPAPKTSNKSFKPTGTSLKRIQKELAEITIDPPPNCSAGPKGDNMYEWVSTILGPPGSVYEGGVFFLDIHFSADYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYTQNRTEHDRVARQWTKRFATGL

Target ORF information:

RefSeq Version	XM_020074114.1
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas ubiquitin-conjugating enzyme E2 E1 (LOC105345258), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020074114.1

ORF Insert Sequence:

ATGAAAAAGA AGTATAGGAT CTACATGACA GTAACAGATA GTGACTCTGC CTGTTCGGGA 
ATGTCTTCAG ATCGGGGAAG CGGTAGGGGT AGGGGCAGAC GATCTCGAGG GGCACAGAAT 
AACGAAGGAA CCCACAGTTC AGGTCCCGAC AGCCCAGCCG AGGCGAACGG CCCCAGCACA 
TCGGCGGTTC AGGCCGAGCC CCCCGCCCCT GCCCCAAAAA CATCCAACAA AAGCTTCAAA 
CCCACGGGCA CCAGTCTAAA ACGAATTCAA AAAGAATTGG CAGAAATCAC CATTGACCCT 
CCGCCGAATT GCAGTGCTGG TCCCAAGGGG GATAACATGT ATGAATGGGT GTCCACCATT 
CTGGGACCCC CAGGGTCTGT GTACGAGGGA GGCGTATTCT TCTTGGACAT TCATTTCTCA 
GCGGATTATC CGTTTAAGCC ACCAAAGGTG ACATTTCGAA CACGGATTTA CCATTGCAAT 
ATAAACAGCC AGGGTGTGAT CTGTCTGGAC ATTCTAAAGG ACAATTGGAG TCCTGCTCTG 
ACTATCTCCA AAGTGTTGTT GTCCATTTGT TCCCTTCTAA CAGACTGTAA TCCGGCCGAC 
CCTCTGGTTG GGAGCATCGC TACTCAGTAC ACTCAGAACA GGACGGAACA CGACAGGGTG 
GCTCGCCAGT GGACCAAGAG GTTCGCCACG GGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.