LOC105343267 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105343267
Entrez Gene ID	105343267
Full Name	glucosylceramidase
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011450565.2	XP_011448867.2	glucosylceramidase

Pathways from BioSystems
Gene Ontology

The following LOC105343267 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105343267 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr110118	XM_011450565.2 Latest version!	Crassostrea gigas glucosylceramidase (LOC105343267), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105343267 ( XM_011450565.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011448867.2 Crassostrea gigas glucosylceramidase (LOC105343267), mRNA.

Price & Availability↑

CloneID	OCr110118
Clone ID Related Accession (Same CDS sequence)	XM_011450565.2
Accession Version	XM_011450565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	glucosylceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936778.1) and transcript sequence (GECI01014193.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011450565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 618 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTATGGTA CCTGTATTCT ATATTCATTG GTTCCATTTT ATCTCGATAT AACAGAGGTC 
TTGGTGAATC TGAACAACAC GTTTCAGTCC ATCATTGGCT TTGGTGGAGC CTTCACTGAT 
GCCGCAGGGA TCAACATCCA GTCCTTGTCG GCCAGCACTC AGAAGAACTT GCTGGCTTCC 
TACTTTTCAA CAGAAGGTAT TGAGTATACA TTGGGAAGAA TTCCTATGGC TAGCTGTGAT 
TTTTCCATGC ATCCATATTC CTATGATGAC AATAGCGGGG ATTTCAATTT GACCAAATTT 
TCGTTAACCC AAGAAGACAA AAAGTTTAAA ATTCCTATCA TACAAGCAGT CATGAGGACA 
TACAAAAGAA ACTTGACATT ATTTGCGAGC CCCTGGAGTG CTCCCGCCTG GATGAAAACC 
AACAAAAACA TGACTGGGAA GGGGACCCTG GTTGGAGAGC CCGGCGGGCC ATACTTCAAG 
ACCTGGGCAA TGTATTTTGT GAAGTTTTTG AAAGCTTATG CTAAGGAAGG TATTCCTATC 
TGGGGTGTAA CCGGGCAGAA CGAGCCTACT GATGGGTTCA TCACAAACTT CTCATTTCAA 
GCTATGGGCT GGACACCTGA AATGCAGAGA GACTTCATTG TGAAGGATCT GGGTCCTGCA 
CTACAACAGA ACTCTCTTGG GCATGTGAAA ATAATGATTC TAGATGACTC CAGACTGCAG 
CTCCCATACT GGGCAGAACA GGTTTTCAGA AGTGAGGAAG CCAGAAAATA TGTATCAGGA 
GTAGCTGTTC ACTGGTATCA GGATTTTATA ACCCCAGCTG TGGCCCTCTC CAGTACCCAT 
AAAAAATTCC CAGAGAAATT CATACTAGCT ACTGAGGCCT GCTCTGGGGC CATGCCATGG 
GATATCCCCA AAGTATCTCT AGGGAGCTGG AAAAGAGGCG AGGACTACGC TCATGATATT 
ATACAGGACT TGAATAACTT TGTAAGTGGG TGGACTGACT GGAACATTGC CCTGAACATG 
GAGGGTGGAC CAAACTGGGT AAAAAACTTT GTAGACAGTC CAGTGATTGT TAATGCTAAG 
AGCGATGAAT TCTACAAGCA ACCTATGTTT TATGCTCTAG GCCATTTCAG CAAGTTTATT 
CTACCCATGT CAAAAAGAGT TGAGCTGGTA CCAAACCAAA ATCTTCCAAA AGATATGTAC 
ATTGTTGGAT TTCAAAGACC AGACAAAGCC ATTGCTTGTA TATTACTCAA CAGATCAAAT 
GAAAATATAA CATTGGCACT TCGAGATCCT TCCACAGGGT ATATAAATAC TGACCTAACA 
CCTCATTCAA TACAGACTGT ATTGTGGTGG CCTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011448867.2
CDS	16..1374
Translation	MYGTCILYSLVPFYLDITEVLVNLNNTFQSIIGFGGAFTDAAGINIQSLSASTQKNLLASYFSTEGIEYTLGRIPMASCDFSMHPYSYDDNSGDFNLTKFSLTQEDKKFKIPIIQAVMRTYKRNLTLFASPWSAPAWMKTNKNMTGKGTLVGEPGGPYFKTWAMYFVKFLKAYAKEGIPIWGVTGQNEPTDGFITNFSFQAMGWTPEMQRDFIVKDLGPALQQNSLGHVKIMILDDSRLQLPYWAEQVFRSEEARKYVSGVAVHWYQDFITPAVALSSTHKKFPEKFILATEACSGAMPWDIPKVSLGSWKRGEDYAHDIIQDLNNFVSGWTDWNIALNMEGGPNWVKNFVDSPVIVNAKSDEFYKQPMFYALGHFSKFILPMSKRVELVPNQNLPKDMYIVGFQRPDKAIACILLNRSNENITLALRDPSTGYINTDLTPHSIQTVLWWPS

Target ORF information:

RefSeq Version	XM_011450565.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas glucosylceramidase (LOC105343267), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011450565.2

ORF Insert Sequence:

ATGTATGGTA CCTGTATTCT ATATTCATTG GTTCCATTTT ATCTCGATAT AACAGAGGTC 
TTGGTGAATC TGAACAACAC GTTTCAGTCC ATCATTGGCT TTGGTGGAGC CTTCACTGAT 
GCCGCAGGGA TCAACATCCA GTCCTTGTCG GCCAGCACTC AGAAGAACTT GCTGGCTTCC 
TACTTTTCAA CAGAAGGTAT TGAGTATACA TTGGGAAGAA TTCCTATGGC TAGCTGTGAT 
TTTTCCATGC ATCCATATTC CTATGATGAC AATAGCGGGG ATTTCAATTT GACCAAATTT 
TCGTTAACCC AAGAAGACAA AAAGTTTAAA ATTCCTATCA TACAAGCAGT CATGAGGACA 
TACAAAAGAA ACTTGACATT ATTTGCGAGC CCCTGGAGTG CTCCCGCCTG GATGAAAACC 
AACAAAAACA TGACTGGGAA GGGGACCCTG GTTGGAGAGC CCGGCGGGCC ATACTTCAAG 
ACCTGGGCAA TGTATTTTGT GAAGTTTTTG AAAGCTTATG CTAAGGAAGG TATTCCTATC 
TGGGGTGTAA CCGGGCAGAA CGAGCCTACT GATGGGTTCA TCACAAACTT CTCATTTCAA 
GCTATGGGCT GGACACCTGA AATGCAGAGA GACTTCATTG TGAAGGATCT GGGTCCTGCA 
CTACAACAGA ACTCTCTTGG GCATGTGAAA ATAATGATTC TAGATGACTC CAGACTGCAG 
CTCCCATACT GGGCAGAACA GGTTTTCAGA AGTGAGGAAG CCAGAAAATA TGTATCAGGA 
GTAGCTGTTC ACTGGTATCA GGATTTTATA ACCCCAGCTG TGGCCCTCTC CAGTACCCAT 
AAAAAATTCC CAGAGAAATT CATACTAGCT ACTGAGGCCT GCTCTGGGGC CATGCCATGG 
GATATCCCCA AAGTATCTCT AGGGAGCTGG AAAAGAGGCG AGGACTACGC TCATGATATT 
ATACAGGACT TGAATAACTT TGTAAGTGGG TGGACTGACT GGAACATTGC CCTGAACATG 
GAGGGTGGAC CAAACTGGGT AAAAAACTTT GTAGACAGTC CAGTGATTGT TAATGCTAAG 
AGCGATGAAT TCTACAAGCA ACCTATGTTT TATGCTCTAG GCCATTTCAG CAAGTTTATT 
CTACCCATGT CAAAAAGAGT TGAGCTGGTA CCAAACCAAA ATCTTCCAAA AGATATGTAC 
ATTGTTGGAT TTCAAAGACC AGACAAAGCC ATTGCTTGTA TATTACTCAA CAGATCAAAT 
GAAAATATAA CATTGGCACT TCGAGATCCT TCCACAGGGT ATATAAATAC TGACCTAACA 
CCTCATTCAA TACAGACTGT ATTGTGGTGG CCTTCCTAG