LOC105333269 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105333269
Entrez Gene ID	105333269
Full Name	alkaline phosphatase
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020069302.1	XP_019924861.1	alkaline phosphatase

Pathways from BioSystems
Gene Ontology

The following LOC105333269 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105333269 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr98811	XM_020069302.1 Latest version!	Crassostrea gigas alkaline phosphatase (LOC105333269), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105333269 ( XM_020069302.1 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_019924861.1 Crassostrea gigas alkaline phosphatase (LOC105333269), mRNA.

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CloneID	OCr98811
Clone ID Related Accession (Same CDS sequence)	XM_020069302.1
Accession Version	XM_020069302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	alkaline phosphatase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011935746.1) and transcript sequence (GECI01002550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 79 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACTATTT TAAAATTCTT GGTAAGTAAA GAACGTCTTG TACACCCACT CAGCGCATGG 
AACACGCCTT TAACAGGAGT CGTAGATAGA GAAGTTAAAG CACAGTCTCC CTGGGGGGAT 
ATTGCGCAGA CGGAACTGGA TGAGGCACTC CGAGTAACTG CCAACACTAA TGTTGCTAAG 
AACGTCATTG TGTTCCTAGG AGACGGGATG AGCATCTCCA CGGTAACGGC CTCCAGGATT 
CTTCAAGGTC GTCTGAAGGC AGAGCTAGGG GAGGGGAACT ATTTAGCATT CGAAAAGTTT 
CCAAATTCAG GCCTGATAAA GACATACTGC AATGATCAAA CAACGCCCGA TTCAGCCTCC 
ACCGCCACCG CCATCTTGTG CGGGGTCAAA ACAAACGCCC GCACACTCGG GGTCAAGGAC 
AGCGCTGTGC TTGGTAACTG TACGTCACAG GCCGGTAATG AAGTGGATAG TATTCTGGAC 
TGGTTCCACG CTGATGGTAG ATCCACAGGT ATCGTAACGA CAGCCAGGAT CACACACGCC 
ACCCCCGGGG CTGGCTACGC CCACTCCGCT GATAGGGTTT GGGAGGGAGA CTCGGAGATG 
GAGGGGGTGA CCGGGGGATG TAAGGACATC GCCTATCAGT TGGTTAAAGA AAATCAACAC 
ATACAGGTAC TCTTTGGGGG TGGTCGCTAT TATTTCCTTC CGAATACTAC AAATGATCCT 
AAACATGGCG ACAAAAATGT GAAAAAACAG AGGAGGGACG GGTTAGATCT AACCAAGGTA 
TGTAACACAC ACACTCTCTC TCTCTGTCTT TTTAATGACG GTCACTTAAG TTTTGAGCTG 
GACAAAGACA GTGATTCAAC CGAAGAACCC AGTCTCGCCG AAATGACGGA AAAGGCGATA 
CGGATTCTCC GTAAAAACCC TAAAGGTTTC TTCTTACTAG TGGAAGGTGC CCGTATTGAT 
TCTGCTCATA CCGATAACAA CGCCAAGAGA GCGTTATATG AAACGCTGGC ATTTGAGACG 
GCGATTAGGG GGGCGTTAGA GCTGGTGGAC ACTAGTAACA CGCTGGTGTC GGTAACGGCG 
GACCACTCGC ACTCGCTCTT AATATCGGGC TATGCTTCTC GTCACAACAG TTTGTTTGGT 
TATGTTGACA AAGTAAGGGC TAAGTATCGT CCAAAAGACG GGGTCCTGTG GACGACACTG 
TTGTACGGCA CTGGACCAGG CTATGATTGG GGACTGAGAA CCAACAAAAC AAAACAGGAG 
TTGGAAAGCA AGGATTACAT CCAAGCCGCC GCCTCGCCGC GGTCTTCCGG TACACACGAC 
GGACAGGATG TGGCAGTGTA TGCACGTGGT CCGATGTCCC ATTTACTAAC AGGCGTGCAC 
GAGCAGCATT ACATCAATTA CGTCATGACA TACGCGGCAT GTGTTGGACG GCACAAACAC 
AAGTGCATGG AAGAATCCAG ACTAATTAAC TCTGCTACTG TGGAGAAATA TGTATTGACC 
TTATATGGTG CTGTCTTTGC CTTGCGTATT CTGTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019924861.1
CDS	1..1539
Translation	MTILKFLVSKERLVHPLSAWNTPLTGVVDREVKAQSPWGDIAQTELDEALRVTANTNVAKNVIVFLGDGMSISTVTASRILQGRLKAELGEGNYLAFEKFPNSGLIKTYCNDQTTPDSASTATAILCGVKTNARTLGVKDSAVLGNCTSQAGNEVDSILDWFHADGRSTGIVTTARITHATPGAGYAHSADRVWEGDSEMEGVTGGCKDIAYQLVKENQHIQVLFGGGRYYFLPNTTNDPKHGDKNVKKQRRDGLDLTKVCNTHTLSLCLFNDGHLSFELDKDSDSTEEPSLAEMTEKAIRILRKNPKGFFLLVEGARIDSAHTDNNAKRALYETLAFETAIRGALELVDTSNTLVSVTADHSHSLLISGYASRHNSLFGYVDKVRAKYRPKDGVLWTTLLYGTGPGYDWGLRTNKTKQELESKDYIQAAASPRSSGTHDGQDVAVYARGPMSHLLTGVHEQHYINYVMTYAACVGRHKHKCMEESRLINSATVEKYVLTLYGAVFALRILY

Target ORF information:

RefSeq Version	XM_020069302.1
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas alkaline phosphatase (LOC105333269), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020069302.1

ORF Insert Sequence:

ATGACTATTT TAAAATTCTT GGTAAGTAAA GAACGTCTTG TACACCCACT CAGCGCATGG 
AACACGCCTT TAACAGGAGT CGTAGATAGA GAAGTTAAAG CACAGTCTCC CTGGGGGGAT 
ATTGCGCAGA CGGAACTGGA TGAGGCACTC CGAGTAACTG CCAACACTAA TGTTGCTAAG 
AACGTCATTG TGTTCCTAGG AGACGGGATG AGCATCTCCA CGGTAACGGC CTCCAGGATT 
CTTCAAGGTC GTCTGAAGGC AGAGCTAGGG GAGGGGAACT ATTTAGCATT CGAAAAGTTT 
CCAAATTCAG GCCTGATAAA GACATACTGC AATGATCAAA CAACGCCCGA TTCAGCCTCC 
ACCGCCACCG CCATCTTGTG CGGGGTCAAA ACAAACGCCC GCACACTCGG GGTCAAGGAC 
AGCGCTGTGC TTGGTAACTG TACGTCACAG GCCGGTAATG AAGTGGATAG TATTCTGGAC 
TGGTTCCACG CTGATGGTAG ATCCACAGGT ATCGTAACGA CAGCCAGGAT CACACACGCC 
ACCCCCGGGG CTGGCTACGC CCACTCCGCT GATAGGGTTT GGGAGGGAGA CTCGGAGATG 
GAGGGGGTGA CCGGGGGATG TAAGGACATC GCCTATCAGT TGGTTAAAGA AAATCAACAC 
ATACAGGTAC TCTTTGGGGG TGGTCGCTAT TATTTCCTTC CGAATACTAC AAATGATCCT 
AAACATGGCG ACAAAAATGT GAAAAAACAG AGGAGGGACG GGTTAGATCT AACCAAGGTA 
TGTAACACAC ACACTCTCTC TCTCTGTCTT TTTAATGACG GTCACTTAAG TTTTGAGCTG 
GACAAAGACA GTGATTCAAC CGAAGAACCC AGTCTCGCCG AAATGACGGA AAAGGCGATA 
CGGATTCTCC GTAAAAACCC TAAAGGTTTC TTCTTACTAG TGGAAGGTGC CCGTATTGAT 
TCTGCTCATA CCGATAACAA CGCCAAGAGA GCGTTATATG AAACGCTGGC ATTTGAGACG 
GCGATTAGGG GGGCGTTAGA GCTGGTGGAC ACTAGTAACA CGCTGGTGTC GGTAACGGCG 
GACCACTCGC ACTCGCTCTT AATATCGGGC TATGCTTCTC GTCACAACAG TTTGTTTGGT 
TATGTTGACA AAGTAAGGGC TAAGTATCGT CCAAAAGACG GGGTCCTGTG GACGACACTG 
TTGTACGGCA CTGGACCAGG CTATGATTGG GGACTGAGAA CCAACAAAAC AAAACAGGAG 
TTGGAAAGCA AGGATTACAT CCAAGCCGCC GCCTCGCCGC GGTCTTCCGG TACACACGAC 
GGACAGGATG TGGCAGTGTA TGCACGTGGT CCGATGTCCC ATTTACTAAC AGGCGTGCAC 
GAGCAGCATT ACATCAATTA CGTCATGACA TACGCGGCAT GTGTTGGACG GCACAAACAC 
AAGTGCATGG AAGAATCCAG ACTAATTAAC TCTGCTACTG TGGAGAAATA TGTATTGACC 
TTATATGGTG CTGTCTTTGC CTTGCGTATT CTGTATTGA