LOC105331513 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105331513
Entrez Gene ID	105331513
Full Name	protein transport protein Sec31A
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011433740.2	XP_011432042.1	protein transport protein Sec31A isoform X1
XM_011433741.2	XP_011432043.1	protein transport protein Sec31A isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC105331513 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105331513 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr96919	XM_011433740.2 Latest version!	Crassostrea gigas protein transport protein Sec31A (LOC105331513), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCr96920	XM_011433741.2 Latest version!	Crassostrea gigas protein transport protein Sec31A (LOC105331513), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105331513 ( XM_011433740.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011432042.1 Crassostrea gigas protein transport protein Sec31A (LOC105331513), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr96919
Clone ID Related Accession (Same CDS sequence)	XM_011433740.2
Accession Version	XM_011433740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	protein transport protein Sec31A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011935585.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011433740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAA AGGAAATTGA GAGGACAGCC AATGTGGCTT GGTCCCCTGC AAAACAACAT 
CCAATTTATA TGGTAGCAGG GACAGCAGCT CAACAGCTTG ATGCTACATT CAGCACATCA 
GCTGCTCTTG AAGTATATCA TCTAAATCTT ACTGACCCTT CCCTCGACAT GCCTTTGGTA 
TCGTCAATAC CATCTGACTG CAGGTTCCAC AAAGTTGTAT GGGGTCCCCA TGGAATGTCA 
GATTCTAGCC ACTCAGCCGG GGTGGTGGTG GCAGGGGCAG ACAATGGAAG TGTTTACTAT 
TATGATGCTG CTGGATTGAT TGAAGGAGAT GAAGATTGTC TTATGTTTAA AAACAATAAG 
CATGTTGGGG CTGTCAAGGC ACTAGATTTC AATCCATTTC AGACAAATTT ACTAGCATCA 
GGGGCATCAG ACTCAGAAAT TTATATTTGG GATTTAAATA AACCAGAAAA CCCAATGACA 
CCTGGGGCAA AATCACAGCC ACCCGAAGAA GTAGCCTGTG TGTCCTGGAA CAAACAAGTT 
CAACACATCC TGGGCTCAAC ATTTGCTGCT AGATGTGTTG TCTGGGATCT CAGAAAGAAT 
GAACCAATCA TTAAGATCAG TGATAGTATG TCAAGGATCA AGTCTAAGAT TGTAAGCTGG 
CACCCTGACG TAGCCACACA GCTGTGTCTG TCCTCTGAGG ATGACCACAC GCCTGTCATA 
CAACTGTGGG ATCTACGCTA TGCCACTTCC CCGGTCAAAC AATTAGAAAA CCATACCAGA 
GGTGTGTTGG ATATTGCCTG GTGTCCACAG GACTCTGATT TGCTGTTAAG TTGTGGAAAA 
GACAACAGAA TTCTATGCTG GAACCCTAAC AGTAATGTTC AAGGAGGAGA GGTTGTGTAT 
GAACTACCTA CGAGTAATCA GTGGTGTTTT GATGTAAAAT GGTGTCCTCG AAATCCAGCC 
GTGATTTCTA GCTCTTCTTT TGATGGGCAT GTTACGGTGT ATTCCCTAAT GGGTGGTGGA 
CATCCAATTC ATCAGAGCAA TAAGGTTGCA GAGGCTTTCA ATTCTAATGA CCCATTTGCA 
CAGGCCACTC CTGTTACTGA AAATACACAA AATGTGATGC CACTTCAGAA ACCACCCAAG 
TGGTTAAAAA AGCCTGTTGG AGTCAACTTT GGGTTTGGTG GTCGCCTTGT AAGCTTTGAG 
AACACAAAGT CCCAGAATCC ACAACAGCCC ACTCCTAAAG TGGTGTACAT TAGCCAGGTG 
GTGACAGAGA CGGACCTTCT ACAGAGGTCC CATCAGCTGG AGCAGGCTCT GGAAAACGGC 
CAGTATGTCG AATATTGTGC CATGAAATCG GCAAACTGTA CAGATCCTCT ACAGGAAAAT 
ATATGGAACT TTCTACGAGT CAACTTCGAA AAGGAACCTA GAGAGCATTT TATTCAGCTT 
TTGGGTTTTG ACAAAACTGA TCTGGCTAAA AAGGTTTCTG AGCACGTGGG TCTCGGACAG 
GATGCTGGAG TAGATGCCAA GGTTCTGGCA CAGAAAATGG CAAAACTTAG TCCAGAGGAT 
AAGCAACAAG CAGGACTAAG AGCCTCAGCA GAAGGCTCTC CAGTGGTTGG ATCTAAGACA 
CCAGTTAGCC AGGATGAAGC TTCATCTGCT TTTGATAATA TTGGTTTATC ACAAAGTACT 
AAATCATTAG ACAAAGAATC CTTGAAACCA CTGGTGATAC CTACTGATCA AGATGCTGAT 
GGGCTTTTGA GTCAGGCCTT GCTTACTGGG AACTTTGAAG CTGCAGTAGA AATCTGTATG 
ATTGAAAACA AAATGGCTGA GGCTATTATC CTGGCTATAG CAGGAGGACC AGATCTCCTG 
AGTAGAACAC AGAAAAGATT CTTTCAGAAG AGCAAATGCA GCATAGGAAG GCTGATATCC 
TCAGTGGTCA CCCATGACTG GTCACATATT GTGGAGACAT GTGACCTCTC CAACTGGAAA 
GAGGCTTTGG CCTTAATCTT AACCTATGCC TCCCCAGATG AATTCCCCAC TCTCTGTGAC 
ACGCTGGCTG AGAGACTGGA GACAGAGAGT GCTGGGGACC TCGGTGTATA TGCCAGTCTA 
TGTTACATCT GTTCAGGAAA TATTGAAAAC CTTCTCAGAA ATTGGTTTGA CAACTCAGAA 
GGTCACAATA CCCCTCTAGG ATTACAGGAC ATGGTAGAGA AGGTGATGTT ACTTAGAAAG 
GCTGTAGAAT TGTCTCGGGG ACAAGTAACA GACATCAGTG GGGGGTTGCT GGCAGAGAAG 
ATGAATGAAT ATGCCAGCCT CCTGGCTGCT CAGGGAAGCT TGGACACAGC TGTCAGATAC 
CTTGGAAATT CTACAGAAGC AAATCTGCTC ATTTTGAAAG ACAGACTGTA CAACTCATTA 
GGTGCTCAGG GAATGCAGTG CCCATTCCAA AAGATCAATG TCTTACCAGC TGGTGCTAAG 
CAGCCCCAGC AGCCTCAGCA ACGCAGCACT CAGGCCCAAA GACAGGCCGG TCAAATAGGG 
ACACGTCAGG CACAGGCCAA GGGCTCGTTT GGCACTGTGA CCACACCCAG TAGTTCTTAC 
AGCAATCCTG GGACTTATAC CACACCGTAC TCCCAGATGA ACGGGACAAA TTCACAACCA 
TCTTACTATA ACCCCGCTGC TAATGTTGCT GCAACACCCC CAATGAGCAG TGCAGGAGTC 
GTGGGGAAAG GGCCATTATC ACACAAGTAC CCCTCGTATC CAGCCACAAC ATCAGCATAT 
GGGATGGACT CGTACTCACC AGCAGGTTTA TATCAGCCAA GCAATCAGCC TTATAATCCA 
GTTCAAACAT TTGATCCATC ACAATATAGT GGAATGGGAG CTTCTAACCA GCCAGCAAGC 
CAGCCAACAA ATATATACCA ACCTCCCTCC AATCCAGGAG GCTCTGCATC ATCCGTATCT 
CCACCTACGA GTGATTACCC AAATGTTCAG TCATTCATGC ACAGCCCACC CTCTTCAGCT 
TGGAATGATC CCCCAATAGT GAAAGATAAG AAGCCAAAGA ATCCTCCAGC ACAGTATGAT 
GGATCGGCCA TGACGAGTCC GTTTTATGGT CAGTCTGTGC CAGCAGATGT TGGTCAGCCG 
GGAGGAGTTA CCAACTACAA TCTGTACAAT CCACAGGAGC ATCAGGCCGA GATACAGCCC 
GCCCAACCTC GGCGTTCATT CCAAAACACC CTTGCTACTC CCGCCACTCA ACAGAAGGCT 
GCAGCCTCTG TTCCGCCCCC AGAACCCCCC AAGCCAGTGG AGAAGGGACC AATTCCATCT 
GAACACAAAG TTTTACAGGA TATATTCACC AGTCTGGTAC TCAATTGCTC CAGCGTGGCA 
TCTAATGCTC AAATGAAAAG AAAGTTAGAC GACGTTTCTA GAAAACTAGA AGTTTTGTAT 
GACAGATTAA GAGAAAACAG ACTCTCCCAG TCTGTGGTTC TAGGCCTGCA TCAGATCGTC 
CAGGCTGTTC AGCAATACGA TTACAACACA GCTCTCCAGA TTTACACTCA GATGATCAGC 
CAAGCCAACT TCTCAGAAAT CAGTAGCTTT ATGCCAGGAC TCAAAGTTTT AATACAGTCA 
GCAATGCAAT TGAATGTTTA TGTGCAAGCT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011432042.1
CDS	197..3832
Translation	MKVKEIERTANVAWSPAKQHPIYMVAGTAAQQLDATFSTSAALEVYHLNLTDPSLDMPLVSSIPSDCRFHKVVWGPHGMSDSSHSAGVVVAGADNGSVYYYDAAGLIEGDEDCLMFKNNKHVGAVKALDFNPFQTNLLASGASDSEIYIWDLNKPENPMTPGAKSQPPEEVACVSWNKQVQHILGSTFAARCVVWDLRKNEPIIKISDSMSRIKSKIVSWHPDVATQLCLSSEDDHTPVIQLWDLRYATSPVKQLENHTRGVLDIAWCPQDSDLLLSCGKDNRILCWNPNSNVQGGEVVYELPTSNQWCFDVKWCPRNPAVISSSSFDGHVTVYSLMGGGHPIHQSNKVAEAFNSNDPFAQATPVTENTQNVMPLQKPPKWLKKPVGVNFGFGGRLVSFENTKSQNPQQPTPKVVYISQVVTETDLLQRSHQLEQALENGQYVEYCAMKSANCTDPLQENIWNFLRVNFEKEPREHFIQLLGFDKTDLAKKVSEHVGLGQDAGVDAKVLAQKMAKLSPEDKQQAGLRASAEGSPVVGSKTPVSQDEASSAFDNIGLSQSTKSLDKESLKPLVIPTDQDADGLLSQALLTGNFEAAVEICMIENKMAEAIILAIAGGPDLLSRTQKRFFQKSKCSIGRLISSVVTHDWSHIVETCDLSNWKEALALILTYASPDEFPTLCDTLAERLETESAGDLGVYASLCYICSGNIENLLRNWFDNSEGHNTPLGLQDMVEKVMLLRKAVELSRGQVTDISGGLLAEKMNEYASLLAAQGSLDTAVRYLGNSTEANLLILKDRLYNSLGAQGMQCPFQKINVLPAGAKQPQQPQQRSTQAQRQAGQIGTRQAQAKGSFGTVTTPSSSYSNPGTYTTPYSQMNGTNSQPSYYNPAANVAATPPMSSAGVVGKGPLSHKYPSYPATTSAYGMDSYSPAGLYQPSNQPYNPVQTFDPSQYSGMGASNQPASQPTNIYQPPSNPGGSASSVSPPTSDYPNVQSFMHSPPSSAWNDPPIVKDKKPKNPPAQYDGSAMTSPFYGQSVPADVGQPGGVTNYNLYNPQEHQAEIQPAQPRRSFQNTLATPATQQKAAASVPPPEPPKPVEKGPIPSEHKVLQDIFTSLVLNCSSVASNAQMKRKLDDVSRKLEVLYDRLRENRLSQSVVLGLHQIVQAVQQYDYNTALQIYTQMISQANFSEISSFMPGLKVLIQSAMQLNVYVQAH

Target ORF information:

RefSeq Version	XM_011433740.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas protein transport protein Sec31A (LOC105331513), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011433740.2

ORF Insert Sequence:

ATGAAAGTAA AGGAAATTGA GAGGACAGCC AATGTGGCTT GGTCCCCTGC AAAACAACAT 
CCAATTTATA TGGTAGCAGG GACAGCAGCT CAACAGCTTG ATGCTACATT CAGCACATCA 
GCTGCTCTTG AAGTATATCA TCTAAATCTT ACTGACCCTT CCCTCGACAT GCCTTTGGTA 
TCGTCAATAC CATCTGACTG CAGGTTCCAC AAAGTTGTAT GGGGTCCCCA TGGAATGTCA 
GATTCTAGCC ACTCAGCCGG GGTGGTGGTG GCAGGGGCAG ACAATGGAAG TGTTTACTAT 
TATGATGCTG CTGGATTGAT TGAAGGAGAT GAAGATTGTC TTATGTTTAA AAACAATAAG 
CATGTTGGGG CTGTCAAGGC ACTAGATTTC AATCCATTTC AGACAAATTT ACTAGCATCA 
GGGGCATCAG ACTCAGAAAT TTATATTTGG GATTTAAATA AACCAGAAAA CCCAATGACA 
CCTGGGGCAA AATCACAGCC ACCCGAAGAA GTAGCCTGTG TGTCCTGGAA CAAACAAGTT 
CAACACATCC TGGGCTCAAC ATTTGCTGCT AGATGTGTTG TCTGGGATCT CAGAAAGAAT 
GAACCAATCA TTAAGATCAG TGATAGTATG TCAAGGATCA AGTCTAAGAT TGTAAGCTGG 
CACCCTGACG TAGCCACACA GCTGTGTCTG TCCTCTGAGG ATGACCACAC GCCTGTCATA 
CAACTGTGGG ATCTACGCTA TGCCACTTCC CCGGTCAAAC AATTAGAAAA CCATACCAGA 
GGTGTGTTGG ATATTGCCTG GTGTCCACAG GACTCTGATT TGCTGTTAAG TTGTGGAAAA 
GACAACAGAA TTCTATGCTG GAACCCTAAC AGTAATGTTC AAGGAGGAGA GGTTGTGTAT 
GAACTACCTA CGAGTAATCA GTGGTGTTTT GATGTAAAAT GGTGTCCTCG AAATCCAGCC 
GTGATTTCTA GCTCTTCTTT TGATGGGCAT GTTACGGTGT ATTCCCTAAT GGGTGGTGGA 
CATCCAATTC ATCAGAGCAA TAAGGTTGCA GAGGCTTTCA ATTCTAATGA CCCATTTGCA 
CAGGCCACTC CTGTTACTGA AAATACACAA AATGTGATGC CACTTCAGAA ACCACCCAAG 
TGGTTAAAAA AGCCTGTTGG AGTCAACTTT GGGTTTGGTG GTCGCCTTGT AAGCTTTGAG 
AACACAAAGT CCCAGAATCC ACAACAGCCC ACTCCTAAAG TGGTGTACAT TAGCCAGGTG 
GTGACAGAGA CGGACCTTCT ACAGAGGTCC CATCAGCTGG AGCAGGCTCT GGAAAACGGC 
CAGTATGTCG AATATTGTGC CATGAAATCG GCAAACTGTA CAGATCCTCT ACAGGAAAAT 
ATATGGAACT TTCTACGAGT CAACTTCGAA AAGGAACCTA GAGAGCATTT TATTCAGCTT 
TTGGGTTTTG ACAAAACTGA TCTGGCTAAA AAGGTTTCTG AGCACGTGGG TCTCGGACAG 
GATGCTGGAG TAGATGCCAA GGTTCTGGCA CAGAAAATGG CAAAACTTAG TCCAGAGGAT 
AAGCAACAAG CAGGACTAAG AGCCTCAGCA GAAGGCTCTC CAGTGGTTGG ATCTAAGACA 
CCAGTTAGCC AGGATGAAGC TTCATCTGCT TTTGATAATA TTGGTTTATC ACAAAGTACT 
AAATCATTAG ACAAAGAATC CTTGAAACCA CTGGTGATAC CTACTGATCA AGATGCTGAT 
GGGCTTTTGA GTCAGGCCTT GCTTACTGGG AACTTTGAAG CTGCAGTAGA AATCTGTATG 
ATTGAAAACA AAATGGCTGA GGCTATTATC CTGGCTATAG CAGGAGGACC AGATCTCCTG 
AGTAGAACAC AGAAAAGATT CTTTCAGAAG AGCAAATGCA GCATAGGAAG GCTGATATCC 
TCAGTGGTCA CCCATGACTG GTCACATATT GTGGAGACAT GTGACCTCTC CAACTGGAAA 
GAGGCTTTGG CCTTAATCTT AACCTATGCC TCCCCAGATG AATTCCCCAC TCTCTGTGAC 
ACGCTGGCTG AGAGACTGGA GACAGAGAGT GCTGGGGACC TCGGTGTATA TGCCAGTCTA 
TGTTACATCT GTTCAGGAAA TATTGAAAAC CTTCTCAGAA ATTGGTTTGA CAACTCAGAA 
GGTCACAATA CCCCTCTAGG ATTACAGGAC ATGGTAGAGA AGGTGATGTT ACTTAGAAAG 
GCTGTAGAAT TGTCTCGGGG ACAAGTAACA GACATCAGTG GGGGGTTGCT GGCAGAGAAG 
ATGAATGAAT ATGCCAGCCT CCTGGCTGCT CAGGGAAGCT TGGACACAGC TGTCAGATAC 
CTTGGAAATT CTACAGAAGC AAATCTGCTC ATTTTGAAAG ACAGACTGTA CAACTCATTA 
GGTGCTCAGG GAATGCAGTG CCCATTCCAA AAGATCAATG TCTTACCAGC TGGTGCTAAG 
CAGCCCCAGC AGCCTCAGCA ACGCAGCACT CAGGCCCAAA GACAGGCCGG TCAAATAGGG 
ACACGTCAGG CACAGGCCAA GGGCTCGTTT GGCACTGTGA CCACACCCAG TAGTTCTTAC 
AGCAATCCTG GGACTTATAC CACACCGTAC TCCCAGATGA ACGGGACAAA TTCACAACCA 
TCTTACTATA ACCCCGCTGC TAATGTTGCT GCAACACCCC CAATGAGCAG TGCAGGAGTC 
GTGGGGAAAG GGCCATTATC ACACAAGTAC CCCTCGTATC CAGCCACAAC ATCAGCATAT 
GGGATGGACT CGTACTCACC AGCAGGTTTA TATCAGCCAA GCAATCAGCC TTATAATCCA 
GTTCAAACAT TTGATCCATC ACAATATAGT GGAATGGGAG CTTCTAACCA GCCAGCAAGC 
CAGCCAACAA ATATATACCA ACCTCCCTCC AATCCAGGAG GCTCTGCATC ATCCGTATCT 
CCACCTACGA GTGATTACCC AAATGTTCAG TCATTCATGC ACAGCCCACC CTCTTCAGCT 
TGGAATGATC CCCCAATAGT GAAAGATAAG AAGCCAAAGA ATCCTCCAGC ACAGTATGAT 
GGATCGGCCA TGACGAGTCC GTTTTATGGT CAGTCTGTGC CAGCAGATGT TGGTCAGCCG 
GGAGGAGTTA CCAACTACAA TCTGTACAAT CCACAGGAGC ATCAGGCCGA GATACAGCCC 
GCCCAACCTC GGCGTTCATT CCAAAACACC CTTGCTACTC CCGCCACTCA ACAGAAGGCT 
GCAGCCTCTG TTCCGCCCCC AGAACCCCCC AAGCCAGTGG AGAAGGGACC AATTCCATCT 
GAACACAAAG TTTTACAGGA TATATTCACC AGTCTGGTAC TCAATTGCTC CAGCGTGGCA 
TCTAATGCTC AAATGAAAAG AAAGTTAGAC GACGTTTCTA GAAAACTAGA AGTTTTGTAT 
GACAGATTAA GAGAAAACAG ACTCTCCCAG TCTGTGGTTC TAGGCCTGCA TCAGATCGTC 
CAGGCTGTTC AGCAATACGA TTACAACACA GCTCTCCAGA TTTACACTCA GATGATCAGC 
CAAGCCAACT TCTCAGAAAT CAGTAGCTTT ATGCCAGGAC TCAAAGTTTT AATACAGTCA 
GCAATGCAAT TGAATGTTTA TGTGCAAGCT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105331513 ( XM_011433741.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011432043.1 Crassostrea gigas protein transport protein Sec31A (LOC105331513), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr96920
Clone ID Related Accession (Same CDS sequence)	XM_011433741.2
Accession Version	XM_011433741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	protein transport protein Sec31A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011935585.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011433741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAA AGGAAATTGA GAGGACAGCC AATGTGGCTT GGTCCCCTGC AAAACAACAT 
CCAATTTATA TGGTAGCAGG GACAGCAGCT CAACAGCTTG ATGCTACATT CAGCACATCA 
GCTGCTCTTG AAGTATATCA TCTAAATCTT ACTGACCCTT CCCTCGACAT GCCTTTGGTA 
TCGTCAATAC CATCTGACTG CAGGTTCCAC AAAGTTGTAT GGGGTCCCCA TGGAATGTCA 
GATTCTAGCC ACTCAGCCGG GGTGGTGGTG GCAGGGGCAG ACAATGGAAG TGTTTACTAT 
TATGATGCTG CTGGATTGAT TGAAGGAGAT GAAGATTGTC TTATGTTTAA AAACAATAAG 
CATGTTGGGG CTGTCAAGGC ACTAGATTTC AATCCATTTC AGACAAATTT ACTAGCATCA 
GGGGCATCAG ACTCAGAAAT TTATATTTGG GATTTAAATA AACCAGAAAA CCCAATGACA 
CCTGGGGCAA AATCACAGCC ACCCGAAGAA GTAGCCTGTG TGTCCTGGAA CAAACAAGTT 
CAACACATCC TGGGCTCAAC ATTTGCTGCT AGATGTGTTG TCTGGGATCT CAGAAAGAAT 
GAACCAATCA TTAAGATCAG TGATAGTATG TCAAGGATCA AGTCTAAGAT TGTAAGCTGG 
CACCCTGACG TAGCCACACA GCTGTGTCTG TCCTCTGAGG ATGACCACAC GCCTGTCATA 
CAACTGTGGG ATCTACGCTA TGCCACTTCC CCGGTCAAAC AATTAGAAAA CCATACCAGA 
GGTGTGTTGG ATATTGCCTG GTGTCCACAG GACTCTGATT TGCTGTTAAG TTGTGGAAAA 
GACAACAGAA TTCTATGCTG GAACCCTAAC AGTAATGTTC AAGGAGGAGA GGTTGTGTAT 
GAACTACCTA CGAGTAATCA GTGGTGTTTT GATGTAAAAT GGTGTCCTCG AAATCCAGCC 
GTGATTTCTA GCTCTTCTTT TGATGGGCAT GTTACGGTGT ATTCCCTAAT GGGTGGTGGA 
CATCCAATTC ATCAGAGCAA TAAGGTTGCA GAGGCTTTCA ATTCTAATGA CCCATTTGCA 
CAGGCCACTC CTGTTACTGA AAATACACAA AATGTGATGC CACTTCAGAA ACCACCCAAG 
TGGTTAAAAA AGCCTGTTGG AGTCAACTTT GGGTTTGGTG GTCGCCTTGT AAGCTTTGAG 
AACACAAAGT CCCAGAATCC ACAACAGCCC ACTCCTAAAG TGGTGTACAT TAGCCAGGTG 
GTGACAGAGA CGGACCTTCT ACAGAGGTCC CATCAGCTGG AGCAGGCTCT GGAAAACGGC 
CAGTATGTCG AATATTGTGC CATGAAATCG GCAAACTGTA CAGATCCTCT ACAGGAAAAT 
ATATGGAACT TTCTACGAGT CAACTTCGAA AAGGAACCTA GAGAGCATTT TATTCAGCTT 
TTGGGTTTTG ACAAAACTGA TCTGGCTAAA AAGGTTTCTG AGCACGTGGG TCTCGGACAG 
GATGCTGGAG TAGATGCCAA GGTTCTGGCA CAGAAAATGG CAAAACTTAG TCCAGAGGAT 
AAGCAACAAG CAGGACTAAG AGCCTCAGCA GAAGGCTCTC CAGTGGTTGG ATCTAAGACA 
CCAGTTAGCC AGGATGAAGC TTCATCTGCT TTTGATAATA TTGGTTTATC ACAAAGTACT 
AAATCATTAG ACAAAGAATC CTTGAAACCA CTGGTGATAC CTACTGATCA AGATGCTGAT 
GGGCTTTTGA GTCAGGCCTT GCTTACTGGG AACTTTGAAG CTGCAGTAGA AATCTGTATG 
ATTGAAAACA AAATGGCTGA GGCTATTATC CTGGCTATAG CAGGAGGACC AGATCTCCTG 
AGTAGAACAC AGAAAAGATT CTTTCAGAAG AGCAAATGCA GCATAGGAAG GCTGATATCC 
TCAGTGGTCA CCCATGACTG GTCACATATT GTGGAGACAT GTGACCTCTC CAACTGGAAA 
GAGGCTTTGG CCTTAATCTT AACCTATGCC TCCCCAGATG AATTCCCCAC TCTCTGTGAC 
ACGCTGGCTG AGAGACTGGA GACAGAGAGT GCTGGGGACC TCGGTGTATA TGCCAGTCTA 
TGTTACATCT GTTCAGGAAA TATTGAAAAC CTTCTCAGAA ATTGGTTTGA CAACTCAGAA 
GGTCACAATA CCCCTCTAGG ATTACAGGAC ATGGTAGAGA AGGTGATGTT ACTTAGAAAG 
GCTGTAGAAT TGTCTCGGGG ACAAGTAACA GACATCAGTG GGGGGTTGCT GGCAGAGAAG 
ATGAATGAAT ATGCCAGCCT CCTGGCTGCT CAGGGAAGCT TGGACACAGC TGTCAGATAC 
CTTGGAAATT CTACAGAAGC AAATCTGCTC ATTTTGAAAG ACAGACTGTA CAACTCATTA 
GGTGCTCAGG GAATGCAGTG CCCATTCCAA AAGATCAATG TCTTACCAGC TGGTGCTAAG 
CAGCCCCAGC AGCCTCAGCA ACGCAGCACT CAGGCCCAAA GACAGGCCGG TCAAATAGGG 
ACACGTCAGG CACAGGCCAA GGGCTCGTTT GGCACTGTGA CCACACCCAG TAGTTCTTAC 
AGCAATCCTG GGACTTATAC CACACCGTAC TCCCAGATGA ACGGGACAAA TTCACAACCA 
TCTTACTATA ACCCCGCTGC TAATGTTGCT GCAACACCCC CAATGAGCAG TGCAGGAGTC 
GTGGGGAAAG GGCCATTATC ACACAAGTAC CCCTCGTATC CAGCCACAAC ATCAGCATAT 
GGGATGGACT CGTACTCACC AGCAGGTTTA TATCAGCCAA GCAATCAGCC TTATAATCCA 
GTTCAAACAT TTGATCCATC ACAATATAGT GGAATGGGAG CTTCTAACCA GCCAGCAAGC 
CAGCCAACAA ATATATACCA ACCTCCCTCC AATCCAGGAG GCTCTGCATC ATCCGTATCT 
CCACCTACGA GTGATTACCC AAATGTTCAG TCATTCATGC ACAGCCCACC CTCTTCAGCT 
TGGAATGATC CCCCAATAGT GAAAGATAAG AAGCCAAAGA ATCCTCCAGC ACAGTATGAT 
GGATCGGCCA TGACGAGTCC GTTTTATGGT CAGTCTGTGC CAGCAGATGT TGGTCAGCCG 
GGAGGAGTTA CCAACTACAA TCTGTACAAT CCACAGGAGC ATCAGGCCGA GATACAGGCT 
GCAGCCTCTG TTCCGCCCCC AGAACCCCCC AAGCCAGTGG AGAAGGGACC AATTCCATCT 
GAACACAAAG TTTTACAGGA TATATTCACC AGTCTGGTAC TCAATTGCTC CAGCGTGGCA 
TCTAATGCTC AAATGAAAAG AAAGTTAGAC GACGTTTCTA GAAAACTAGA AGTTTTGTAT 
GACAGATTAA GAGAAAACAG ACTCTCCCAG TCTGTGGTTC TAGGCCTGCA TCAGATCGTC 
CAGGCTGTTC AGCAATACGA TTACAACACA GCTCTCCAGA TTTACACTCA GATGATCAGC 
CAAGCCAACT TCTCAGAAAT CAGTAGCTTT ATGCCAGGAC TCAAAGTTTT AATACAGTCA 
GCAATGCAAT TGAATGTTTA TGTGCAAGCT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011432043.1
CDS	196..3771
Translation	MKVKEIERTANVAWSPAKQHPIYMVAGTAAQQLDATFSTSAALEVYHLNLTDPSLDMPLVSSIPSDCRFHKVVWGPHGMSDSSHSAGVVVAGADNGSVYYYDAAGLIEGDEDCLMFKNNKHVGAVKALDFNPFQTNLLASGASDSEIYIWDLNKPENPMTPGAKSQPPEEVACVSWNKQVQHILGSTFAARCVVWDLRKNEPIIKISDSMSRIKSKIVSWHPDVATQLCLSSEDDHTPVIQLWDLRYATSPVKQLENHTRGVLDIAWCPQDSDLLLSCGKDNRILCWNPNSNVQGGEVVYELPTSNQWCFDVKWCPRNPAVISSSSFDGHVTVYSLMGGGHPIHQSNKVAEAFNSNDPFAQATPVTENTQNVMPLQKPPKWLKKPVGVNFGFGGRLVSFENTKSQNPQQPTPKVVYISQVVTETDLLQRSHQLEQALENGQYVEYCAMKSANCTDPLQENIWNFLRVNFEKEPREHFIQLLGFDKTDLAKKVSEHVGLGQDAGVDAKVLAQKMAKLSPEDKQQAGLRASAEGSPVVGSKTPVSQDEASSAFDNIGLSQSTKSLDKESLKPLVIPTDQDADGLLSQALLTGNFEAAVEICMIENKMAEAIILAIAGGPDLLSRTQKRFFQKSKCSIGRLISSVVTHDWSHIVETCDLSNWKEALALILTYASPDEFPTLCDTLAERLETESAGDLGVYASLCYICSGNIENLLRNWFDNSEGHNTPLGLQDMVEKVMLLRKAVELSRGQVTDISGGLLAEKMNEYASLLAAQGSLDTAVRYLGNSTEANLLILKDRLYNSLGAQGMQCPFQKINVLPAGAKQPQQPQQRSTQAQRQAGQIGTRQAQAKGSFGTVTTPSSSYSNPGTYTTPYSQMNGTNSQPSYYNPAANVAATPPMSSAGVVGKGPLSHKYPSYPATTSAYGMDSYSPAGLYQPSNQPYNPVQTFDPSQYSGMGASNQPASQPTNIYQPPSNPGGSASSVSPPTSDYPNVQSFMHSPPSSAWNDPPIVKDKKPKNPPAQYDGSAMTSPFYGQSVPADVGQPGGVTNYNLYNPQEHQAEIQAAASVPPPEPPKPVEKGPIPSEHKVLQDIFTSLVLNCSSVASNAQMKRKLDDVSRKLEVLYDRLRENRLSQSVVLGLHQIVQAVQQYDYNTALQIYTQMISQANFSEISSFMPGLKVLIQSAMQLNVYVQAH

Target ORF information:

RefSeq Version	XM_011433741.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas protein transport protein Sec31A (LOC105331513), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011433741.2

ORF Insert Sequence:

ATGAAAGTAA AGGAAATTGA GAGGACAGCC AATGTGGCTT GGTCCCCTGC AAAACAACAT 
CCAATTTATA TGGTAGCAGG GACAGCAGCT CAACAGCTTG ATGCTACATT CAGCACATCA 
GCTGCTCTTG AAGTATATCA TCTAAATCTT ACTGACCCTT CCCTCGACAT GCCTTTGGTA 
TCGTCAATAC CATCTGACTG CAGGTTCCAC AAAGTTGTAT GGGGTCCCCA TGGAATGTCA 
GATTCTAGCC ACTCAGCCGG GGTGGTGGTG GCAGGGGCAG ACAATGGAAG TGTTTACTAT 
TATGATGCTG CTGGATTGAT TGAAGGAGAT GAAGATTGTC TTATGTTTAA AAACAATAAG 
CATGTTGGGG CTGTCAAGGC ACTAGATTTC AATCCATTTC AGACAAATTT ACTAGCATCA 
GGGGCATCAG ACTCAGAAAT TTATATTTGG GATTTAAATA AACCAGAAAA CCCAATGACA 
CCTGGGGCAA AATCACAGCC ACCCGAAGAA GTAGCCTGTG TGTCCTGGAA CAAACAAGTT 
CAACACATCC TGGGCTCAAC ATTTGCTGCT AGATGTGTTG TCTGGGATCT CAGAAAGAAT 
GAACCAATCA TTAAGATCAG TGATAGTATG TCAAGGATCA AGTCTAAGAT TGTAAGCTGG 
CACCCTGACG TAGCCACACA GCTGTGTCTG TCCTCTGAGG ATGACCACAC GCCTGTCATA 
CAACTGTGGG ATCTACGCTA TGCCACTTCC CCGGTCAAAC AATTAGAAAA CCATACCAGA 
GGTGTGTTGG ATATTGCCTG GTGTCCACAG GACTCTGATT TGCTGTTAAG TTGTGGAAAA 
GACAACAGAA TTCTATGCTG GAACCCTAAC AGTAATGTTC AAGGAGGAGA GGTTGTGTAT 
GAACTACCTA CGAGTAATCA GTGGTGTTTT GATGTAAAAT GGTGTCCTCG AAATCCAGCC 
GTGATTTCTA GCTCTTCTTT TGATGGGCAT GTTACGGTGT ATTCCCTAAT GGGTGGTGGA 
CATCCAATTC ATCAGAGCAA TAAGGTTGCA GAGGCTTTCA ATTCTAATGA CCCATTTGCA 
CAGGCCACTC CTGTTACTGA AAATACACAA AATGTGATGC CACTTCAGAA ACCACCCAAG 
TGGTTAAAAA AGCCTGTTGG AGTCAACTTT GGGTTTGGTG GTCGCCTTGT AAGCTTTGAG 
AACACAAAGT CCCAGAATCC ACAACAGCCC ACTCCTAAAG TGGTGTACAT TAGCCAGGTG 
GTGACAGAGA CGGACCTTCT ACAGAGGTCC CATCAGCTGG AGCAGGCTCT GGAAAACGGC 
CAGTATGTCG AATATTGTGC CATGAAATCG GCAAACTGTA CAGATCCTCT ACAGGAAAAT 
ATATGGAACT TTCTACGAGT CAACTTCGAA AAGGAACCTA GAGAGCATTT TATTCAGCTT 
TTGGGTTTTG ACAAAACTGA TCTGGCTAAA AAGGTTTCTG AGCACGTGGG TCTCGGACAG 
GATGCTGGAG TAGATGCCAA GGTTCTGGCA CAGAAAATGG CAAAACTTAG TCCAGAGGAT 
AAGCAACAAG CAGGACTAAG AGCCTCAGCA GAAGGCTCTC CAGTGGTTGG ATCTAAGACA 
CCAGTTAGCC AGGATGAAGC TTCATCTGCT TTTGATAATA TTGGTTTATC ACAAAGTACT 
AAATCATTAG ACAAAGAATC CTTGAAACCA CTGGTGATAC CTACTGATCA AGATGCTGAT 
GGGCTTTTGA GTCAGGCCTT GCTTACTGGG AACTTTGAAG CTGCAGTAGA AATCTGTATG 
ATTGAAAACA AAATGGCTGA GGCTATTATC CTGGCTATAG CAGGAGGACC AGATCTCCTG 
AGTAGAACAC AGAAAAGATT CTTTCAGAAG AGCAAATGCA GCATAGGAAG GCTGATATCC 
TCAGTGGTCA CCCATGACTG GTCACATATT GTGGAGACAT GTGACCTCTC CAACTGGAAA 
GAGGCTTTGG CCTTAATCTT AACCTATGCC TCCCCAGATG AATTCCCCAC TCTCTGTGAC 
ACGCTGGCTG AGAGACTGGA GACAGAGAGT GCTGGGGACC TCGGTGTATA TGCCAGTCTA 
TGTTACATCT GTTCAGGAAA TATTGAAAAC CTTCTCAGAA ATTGGTTTGA CAACTCAGAA 
GGTCACAATA CCCCTCTAGG ATTACAGGAC ATGGTAGAGA AGGTGATGTT ACTTAGAAAG 
GCTGTAGAAT TGTCTCGGGG ACAAGTAACA GACATCAGTG GGGGGTTGCT GGCAGAGAAG 
ATGAATGAAT ATGCCAGCCT CCTGGCTGCT CAGGGAAGCT TGGACACAGC TGTCAGATAC 
CTTGGAAATT CTACAGAAGC AAATCTGCTC ATTTTGAAAG ACAGACTGTA CAACTCATTA 
GGTGCTCAGG GAATGCAGTG CCCATTCCAA AAGATCAATG TCTTACCAGC TGGTGCTAAG 
CAGCCCCAGC AGCCTCAGCA ACGCAGCACT CAGGCCCAAA GACAGGCCGG TCAAATAGGG 
ACACGTCAGG CACAGGCCAA GGGCTCGTTT GGCACTGTGA CCACACCCAG TAGTTCTTAC 
AGCAATCCTG GGACTTATAC CACACCGTAC TCCCAGATGA ACGGGACAAA TTCACAACCA 
TCTTACTATA ACCCCGCTGC TAATGTTGCT GCAACACCCC CAATGAGCAG TGCAGGAGTC 
GTGGGGAAAG GGCCATTATC ACACAAGTAC CCCTCGTATC CAGCCACAAC ATCAGCATAT 
GGGATGGACT CGTACTCACC AGCAGGTTTA TATCAGCCAA GCAATCAGCC TTATAATCCA 
GTTCAAACAT TTGATCCATC ACAATATAGT GGAATGGGAG CTTCTAACCA GCCAGCAAGC 
CAGCCAACAA ATATATACCA ACCTCCCTCC AATCCAGGAG GCTCTGCATC ATCCGTATCT 
CCACCTACGA GTGATTACCC AAATGTTCAG TCATTCATGC ACAGCCCACC CTCTTCAGCT 
TGGAATGATC CCCCAATAGT GAAAGATAAG AAGCCAAAGA ATCCTCCAGC ACAGTATGAT 
GGATCGGCCA TGACGAGTCC GTTTTATGGT CAGTCTGTGC CAGCAGATGT TGGTCAGCCG 
GGAGGAGTTA CCAACTACAA TCTGTACAAT CCACAGGAGC ATCAGGCCGA GATACAGGCT 
GCAGCCTCTG TTCCGCCCCC AGAACCCCCC AAGCCAGTGG AGAAGGGACC AATTCCATCT 
GAACACAAAG TTTTACAGGA TATATTCACC AGTCTGGTAC TCAATTGCTC CAGCGTGGCA 
TCTAATGCTC AAATGAAAAG AAAGTTAGAC GACGTTTCTA GAAAACTAGA AGTTTTGTAT 
GACAGATTAA GAGAAAACAG ACTCTCCCAG TCTGTGGTTC TAGGCCTGCA TCAGATCGTC 
CAGGCTGTTC AGCAATACGA TTACAACACA GCTCTCCAGA TTTACACTCA GATGATCAGC 
CAAGCCAACT TCTCAGAAAT CAGTAGCTTT ATGCCAGGAC TCAAAGTTTT AATACAGTCA 
GCAATGCAAT TGAATGTTTA TGTGCAAGCT CATTAG