Pepck cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pepck
Entrez Gene ID	105326107
Full Name	phosphoenolpyruvate carboxykinase
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305293.1	NP_001292222.1	uncharacterized protein LOC105326107

Pathways from BioSystems
Gene Ontology

The following Pepck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pepck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr05862	NM_001305293.1 Latest version!	Crassostrea gigas phosphoenolpyruvate carboxykinase (Pepck), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pepck ( NM_001305293.1 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> NP_001292222.1 Crassostrea gigas phosphoenolpyruvate carboxykinase (Pepck), mRNA.

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CloneID	OCr05862
Clone ID Related Accession (Same CDS sequence)	NM_001305293.1
Accession Version	NM_001305293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Crassostrea gigas(Pacific oyster)
Product	uncharacterized protein LOC105326107
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from AM076952.1. ##Evidence-Data-START## Transcript exon combination :: AM076952.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00714285, SAMN00714286 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGACG ACGCACCAGA GTTCATGGAA ATTCACGAGA TCGTGATACA AAAGCTGGGT 
CATGTCCCGA TCGTCAAAGG CGATTTCCAC ATGCTGCCCA AGAAAGTGCA GAAGTTCTTG 
GCAAAATGGG TGTACACGTG TAAGCCACGC GCTCTGTATA TCTGTGATGG GTCTCATGCA 
GAAGCTGAGG AGGTCACCCA CAAGCTGATA GAGAGGGGGG TCCTCACAAA ATTGAAGAAA 
TACGAAAACT GCTACTTGTG CCGTACTGAC CCAGCTGATG TTGCTCGGGT GGAATCAAAA 
ACGTGGATTG CAACTGATGA TAAATATGAA ACAGTCCCCC ATGTGCGACA GGGAGTGAGG 
GGAATTCTGG GACAATGGAA ACACACCAAG GAAATGGAGG AGGAGGTGAA TAGTGATTTG 
GATGGCTGCA TGGCAGGTAG GACTATGTAT GTGATCCCCT TCAGTATGGG GCCCATCGGG 
GGTCCCCTGA GTAAGATAGG GGTCCAGCTG ACAGATTCCA ACTACGTCCT GCTCTGTATG 
AGAATTATGA CCAGAGTGTC AGCGGATATC TGGGAGGTTC TGGGAGACAA TGACTTTGTG 
AGATGTGTGC ATTCCATGGG CTGTCCCAGA CCAGTGCAGA GAAAGGTCGT CAACCACTGG 
CCATGTAACC CAGACAAGAT CATGATCGGT CATTTTCCGG CCCGCCGTGA GATTGTGTCC 
TTTGGTAGTG GTTACGGAGG TAACTCATTG CTGGGGAAGA AGTGCTTTGC TCTGAGAATT 
GCCAGTGTCA TTGCCAGGGA TGAGGGATGG CTCGCCGAGC ACATGTTGAT TATGGGATTG 
ACCAATGAGA AAACAGGAGA AGAGAAATTT GTTTGTGCAG CTTTCCCGAG TGCCTGTGGG 
AAGACCAATC TTGCCATGAT CCAGCCAACC ATCCCAGGGT ACAAAGTTCG AGTTGTCGGG 
GATGACATTG CGTGGCTTAG ATTTGACGAT AAGGGTGTCC TTAGAGCCAT CAACCCCGAG 
AACGGCTTCT TAGGAGTGCT CCCAGGCACC AACATGAAAA CTAACCCCAA TGCCATGCTG 
TCATTTCAGA ATATCTCCAT CTTCACAAAT GTGGCAGAAA CAGCAGATGG CGGGGTCTTC 
TGGGAGGGAA TGGAGGATGA GATTGATAAA AACATTGCCA TCACTAACTG GCTGGGACAG 
CCATGGAAGA TAGGAATGCC AGGCAATGCT GCCCACCCCA ACTCCCGCTT CACCTGCCCG 
GCGTCCCAGT GCCCCATCAT CCACCCAAAG TGGGAGGATC CGAAGGGAGT GCCAGTCAGC 
GCTCTCATCT TTGGGGGACG ACGTCCCACA GGTGTACCCC TGGTGTTTGA ATCCTACAGC 
TGGCAACATG GCGTCATGGT TGGAGCCTGC GTCAAATCTG AGTCCACTGC TGCCGCAGAA 
CATACAGGAA AAAAAATCAT GCATGACCCA ATGGCAATGA GGCCATTTAT GGGATACAAT 
TTTGGAAAAT ACCTACAGCA CTGGCTTGAC ATGGAGAAAC CAGACAGAAA TATGCCCAAG 
AGCTTCCATG TGAATTGGTT CCGGGTGGAT GAGCATGGGA AATTCCTGTG GCCTGGATTT 
GGAGACAATG TGCGGGTGTT GGACTGGGTC CTGAGGAGAT GTGCTGGAGA TGAATCCATT 
GCTGTGAAAT CTCCAATTGG TTACCTTCCC AAGAAAGGCA GCATTGACCT GACTGGGCTG 
GGCGACATTA AGTGGGACGA GCTGTTCAGC CTGCCCAAGC ACTACTGGCT GGACGACATG 
CGTGAGAGCA AGCTCTTCCT GGAGGACCAG GTCGGCACGG ACTTTCCTCA GGTCATCTGG 
GAGACTCACG CCCAGGAGAA GCGCATAGAG GAGCAGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292222.1
CDS	103..2004
Translation	MEDDAPEFMEIHEIVIQKLGHVPIVKGDFHMLPKKVQKFLAKWVYTCKPRALYICDGSHAEAEEVTHKLIERGVLTKLKKYENCYLCRTDPADVARVESKTWIATDDKYETVPHVRQGVRGILGQWKHTKEMEEEVNSDLDGCMAGRTMYVIPFSMGPIGGPLSKIGVQLTDSNYVLLCMRIMTRVSADIWEVLGDNDFVRCVHSMGCPRPVQRKVVNHWPCNPDKIMIGHFPARREIVSFGSGYGGNSLLGKKCFALRIASVIARDEGWLAEHMLIMGLTNEKTGEEKFVCAAFPSACGKTNLAMIQPTIPGYKVRVVGDDIAWLRFDDKGVLRAINPENGFLGVLPGTNMKTNPNAMLSFQNISIFTNVAETADGGVFWEGMEDEIDKNIAITNWLGQPWKIGMPGNAAHPNSRFTCPASQCPIIHPKWEDPKGVPVSALIFGGRRPTGVPLVFESYSWQHGVMVGACVKSESTAAAEHTGKKIMHDPMAMRPFMGYNFGKYLQHWLDMEKPDRNMPKSFHVNWFRVDEHGKFLWPGFGDNVRVLDWVLRRCAGDESIAVKSPIGYLPKKGSIDLTGLGDIKWDELFSLPKHYWLDDMRESKLFLEDQVGTDFPQVIWETHAQEKRIEEQL

Target ORF information:

RefSeq Version	NM_001305293.1
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas phosphoenolpyruvate carboxykinase (Pepck), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305293.1

ORF Insert Sequence:

ATGGAGGACG ACGCACCAGA GTTCATGGAA ATTCACGAGA TCGTGATACA AAAGCTGGGT 
CATGTCCCGA TCGTCAAAGG CGATTTCCAC ATGCTGCCCA AGAAAGTGCA GAAGTTCTTG 
GCAAAATGGG TGTACACGTG TAAGCCACGC GCTCTGTATA TCTGTGATGG GTCTCATGCA 
GAAGCTGAGG AGGTCACCCA CAAGCTGATA GAGAGGGGGG TCCTCACAAA ATTGAAGAAA 
TACGAAAACT GCTACTTGTG CCGTACTGAC CCAGCTGATG TTGCTCGGGT GGAATCAAAA 
ACGTGGATTG CAACTGATGA TAAATATGAA ACAGTCCCCC ATGTGCGACA GGGAGTGAGG 
GGAATTCTGG GACAATGGAA ACACACCAAG GAAATGGAGG AGGAGGTGAA TAGTGATTTG 
GATGGCTGCA TGGCAGGTAG GACTATGTAT GTGATCCCCT TCAGTATGGG GCCCATCGGG 
GGTCCCCTGA GTAAGATAGG GGTCCAGCTG ACAGATTCCA ACTACGTCCT GCTCTGTATG 
AGAATTATGA CCAGAGTGTC AGCGGATATC TGGGAGGTTC TGGGAGACAA TGACTTTGTG 
AGATGTGTGC ATTCCATGGG CTGTCCCAGA CCAGTGCAGA GAAAGGTCGT CAACCACTGG 
CCATGTAACC CAGACAAGAT CATGATCGGT CATTTTCCGG CCCGCCGTGA GATTGTGTCC 
TTTGGTAGTG GTTACGGAGG TAACTCATTG CTGGGGAAGA AGTGCTTTGC TCTGAGAATT 
GCCAGTGTCA TTGCCAGGGA TGAGGGATGG CTCGCCGAGC ACATGTTGAT TATGGGATTG 
ACCAATGAGA AAACAGGAGA AGAGAAATTT GTTTGTGCAG CTTTCCCGAG TGCCTGTGGG 
AAGACCAATC TTGCCATGAT CCAGCCAACC ATCCCAGGGT ACAAAGTTCG AGTTGTCGGG 
GATGACATTG CGTGGCTTAG ATTTGACGAT AAGGGTGTCC TTAGAGCCAT CAACCCCGAG 
AACGGCTTCT TAGGAGTGCT CCCAGGCACC AACATGAAAA CTAACCCCAA TGCCATGCTG 
TCATTTCAGA ATATCTCCAT CTTCACAAAT GTGGCAGAAA CAGCAGATGG CGGGGTCTTC 
TGGGAGGGAA TGGAGGATGA GATTGATAAA AACATTGCCA TCACTAACTG GCTGGGACAG 
CCATGGAAGA TAGGAATGCC AGGCAATGCT GCCCACCCCA ACTCCCGCTT CACCTGCCCG 
GCGTCCCAGT GCCCCATCAT CCACCCAAAG TGGGAGGATC CGAAGGGAGT GCCAGTCAGC 
GCTCTCATCT TTGGGGGACG ACGTCCCACA GGTGTACCCC TGGTGTTTGA ATCCTACAGC 
TGGCAACATG GCGTCATGGT TGGAGCCTGC GTCAAATCTG AGTCCACTGC TGCCGCAGAA 
CATACAGGAA AAAAAATCAT GCATGACCCA ATGGCAATGA GGCCATTTAT GGGATACAAT 
TTTGGAAAAT ACCTACAGCA CTGGCTTGAC ATGGAGAAAC CAGACAGAAA TATGCCCAAG 
AGCTTCCATG TGAATTGGTT CCGGGTGGAT GAGCATGGGA AATTCCTGTG GCCTGGATTT 
GGAGACAATG TGCGGGTGTT GGACTGGGTC CTGAGGAGAT GTGCTGGAGA TGAATCCATT 
GCTGTGAAAT CTCCAATTGG TTACCTTCCC AAGAAAGGCA GCATTGACCT GACTGGGCTG 
GGCGACATTA AGTGGGACGA GCTGTTCAGC CTGCCCAAGC ACTACTGGCT GGACGACATG 
CGTGAGAGCA AGCTCTTCCT GGAGGACCAG GTCGGCACGG ACTTTCCTCA GGTCATCTGG 
GAGACTCACG CCCAGGAGAA GCGCATAGAG GAGCAGCTGT AG