SIRT1 cDNA ORF clone, Corvus brachyrhynchos(American crow)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIRT1
Entrez Gene ID	103623727
Full Name	sirtuin 1
Gene Type	protein-coding
Organism	Corvus brachyrhynchos(American crow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008642876.1	XP_008641098.1	NAD-dependent protein deacetylase sirtuin-1

Pathways from BioSystems
Gene Ontology

The following SIRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo56068	XM_008642876.1 Latest version!	Corvus brachyrhynchos sirtuin 1 (SIRT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIRT1 ( XM_008642876.1 ) cDNA ORF clone, Corvus brachyrhynchos(American crow) -> XP_008641098.1 Corvus brachyrhynchos sirtuin 1 (SIRT1), mRNA.

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CloneID	OCo56068
Clone ID Related Accession (Same CDS sequence)	XM_008642876.1
Accession Version	XM_008642876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-02
Organism	Corvus brachyrhynchos(American crow)
Product	NAD-dependent protein deacetylase sirtuin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008246060.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Corvus brachyrhynchos Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGAAAATC CACGCGTGGC ACCTCCTGTT CGCTGTGCAG TGATGGTCCC GCTCCAGCGC 
CCAGATATAC CTGTGCCCTT GGGCTGGAGT GTAACCCCCT GGTTGTGCTG TCTTGCAGAA 
AACTTCCTCT TCAGCGATGA AATCATAGCC AATGGTTTTC ACTCCTGTGA CAGTGATGAA 
GAAGACAGAG CCTCACATGC AAGTTCCAGT GACTGGACCC CAAGACCACG TATAGGTCCC 
TACACTTTTG TTCAGCAGCA TCTCATGTTA GGCACAGACC CACGGACGAT TCTGAAGGAC 
CTGCTGCCAG AAACGATCCC CCCACCTGAA CTGGATGACA TGACTCTGTG GCAAATTGTG 
ATAAACATCC TTTCAGAGCC ACCAAAAAGG AAGAAGAGGA AAGATATTAA CACCATTGAT 
GATGCTGTGA AACTTTTGCA GGAATGCAAG AAGATCATGG TCTTGACTGG AGCTGGGGTG 
TCTGTGTCTT GTGGAATACC TGACTTTAGA TCCAGGGATG GCATCTATGC ACGCCTTGCT 
GTAGACTTCC CAGACCTTCC AGATCCTCAA GCAATGTTTG ATATAGAATA CTTCAGAAAG 
GATCCCAGGC CGTTTTTTAA GTTCGCAAAG GAAATCTACC CGGGACAATT CCAGCCATCT 
CTGTGCCACA AGTTCATCGC TTTGATGGAT AAAGAAGGAA AACTACTTCG CAACTATACT 
CAGAACATAG ACACACTGGA ACAGGTTGCA GGAATCCAAA GGATAATACA ATGTCATGGT 
TCCTTTGCAA CAGCTTCCTG CCTGATCTGT AAATACAAAG TTGATTGTGA AGTTGTTCGA 
GGAGATATTT TCAATCAGGT GGTGCCCCGG TGTCCCCGCT GCCCCCCCGA GGAGCCGCTG 
GCTATCATGA AGCCGGACAT TGTGTTCTTT GGGGAGAACC TGCCTGAGCA GTTCCACCGT 
GCCATGAAGT ATGACAAAAA TGAAGTGGAT CTCCTTATTG TCATTGGGTC TTCACTCAAA 
GTAAGACCAG TAGCATTGAT CCCAAGTTCC ATCCCCCATG AAGTGCCTCA GATCTTAATT 
AATAGGGAAC CTTTGCCTCA TCTACACTTT GACGTGGAGC TTCTCGGAGA CTGTGATGTT 
ATTATTAATG AATTATGTCA GAGGTTAGGT AGCGAATACA CAAAACTTTG CTACAACTCA 
GTAAAACTTT CAGAAATCAC AGAAAAGCCT CCACGGCCGC ACAAGGAGCT CGAAGTGCTC 
TCAGCTGAGC TCCCACCAAC CCCTCTGAAC ATTTCAGAAG GCTCCAGTTC ACCTGAAAGA 
ATGACCCCAC CTGATACTGC AGTGGTGTCT TCAGAACACC CACCTGAATG TAAGGTAGAA 
AACTGTGAGC CTGCCTCAGA GACTAAAGGG ACCTGCTCAG AGGACACGCT TCAGGACACA 
GAGCTGGCAT CAGAAAACCC TGAAAATCCT GCTGGTGAGC TAATGAACTC TGAAACAATG 
AAGGAAAATG GATCTAATGA TGGAGAAAAT AAAGAAAAGA GTGAAATACT GAAGAAGTGC 
TGGGTAAACA GATCCGCGAA AGAACAGATT AGCAAAAGGC TGGATGGTAC TCAGTATCTG 
TTTTTGCCAC CCAATCGCTA TATCTTCCAT GGTGCTGAGG TTTACTCGGA TTCTGAAGAC 
GATATCATAT CTTCCAGCTC TTGTGGCAGC AGTAGTGAGA GCGGCTCGTG CCGCAGTCAG 
AGCTTAGATG TAGAGGACGA GAGTGAGATG GAGGAGTTTT ACAATGGCAT AGATGAGGAT 
GCTCCCGAGC AGGAAGAGGA GCCTGGATTC GGAGAGGATG GAGCCGAACG GGAGGAATGG 
GCAGCTGAGG AATCAGCTGA TACAAATGAA GCTGCAGGGA CTGAACATCC GAGCAATGCG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008641098.1
CDS	1..1926
Translation	MENPRVAPPVRCAVMVPLQRPDIPVPLGWSVTPWLCCLAENFLFSDEIIANGFHSCDSDEEDRASHASSSDWTPRPRIGPYTFVQQHLMLGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIDDAVKLLQECKKIMVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALMDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEVVRGDIFNQVVPRCPRCPPEEPLAIMKPDIVFFGENLPEQFHRAMKYDKNEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCQRLGSEYTKLCYNSVKLSEITEKPPRPHKELEVLSAELPPTPLNISEGSSSPERMTPPDTAVVSSEHPPECKVENCEPASETKGTCSEDTLQDTELASENPENPAGELMNSETMKENGSNDGENKEKSEILKKCWVNRSAKEQISKRLDGTQYLFLPPNRYIFHGAEVYSDSEDDIISSSSCGSSSESGSCRSQSLDVEDESEMEEFYNGIDEDAPEQEEEPGFGEDGAEREEWAAEESADTNEAAGTEHPSNAL

Target ORF information:

RefSeq Version	XM_008642876.1
Organism	Corvus brachyrhynchos(American crow)
Definition	Corvus brachyrhynchos sirtuin 1 (SIRT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008642876.1

ORF Insert Sequence:

ATGGAAAATC CACGCGTGGC ACCTCCTGTT CGCTGTGCAG TGATGGTCCC GCTCCAGCGC 
CCAGATATAC CTGTGCCCTT GGGCTGGAGT GTAACCCCCT GGTTGTGCTG TCTTGCAGAA 
AACTTCCTCT TCAGCGATGA AATCATAGCC AATGGTTTTC ACTCCTGTGA CAGTGATGAA 
GAAGACAGAG CCTCACATGC AAGTTCCAGT GACTGGACCC CAAGACCACG TATAGGTCCC 
TACACTTTTG TTCAGCAGCA TCTCATGTTA GGCACAGACC CACGGACGAT TCTGAAGGAC 
CTGCTGCCAG AAACGATCCC CCCACCTGAA CTGGATGACA TGACTCTGTG GCAAATTGTG 
ATAAACATCC TTTCAGAGCC ACCAAAAAGG AAGAAGAGGA AAGATATTAA CACCATTGAT 
GATGCTGTGA AACTTTTGCA GGAATGCAAG AAGATCATGG TCTTGACTGG AGCTGGGGTG 
TCTGTGTCTT GTGGAATACC TGACTTTAGA TCCAGGGATG GCATCTATGC ACGCCTTGCT 
GTAGACTTCC CAGACCTTCC AGATCCTCAA GCAATGTTTG ATATAGAATA CTTCAGAAAG 
GATCCCAGGC CGTTTTTTAA GTTCGCAAAG GAAATCTACC CGGGACAATT CCAGCCATCT 
CTGTGCCACA AGTTCATCGC TTTGATGGAT AAAGAAGGAA AACTACTTCG CAACTATACT 
CAGAACATAG ACACACTGGA ACAGGTTGCA GGAATCCAAA GGATAATACA ATGTCATGGT 
TCCTTTGCAA CAGCTTCCTG CCTGATCTGT AAATACAAAG TTGATTGTGA AGTTGTTCGA 
GGAGATATTT TCAATCAGGT GGTGCCCCGG TGTCCCCGCT GCCCCCCCGA GGAGCCGCTG 
GCTATCATGA AGCCGGACAT TGTGTTCTTT GGGGAGAACC TGCCTGAGCA GTTCCACCGT 
GCCATGAAGT ATGACAAAAA TGAAGTGGAT CTCCTTATTG TCATTGGGTC TTCACTCAAA 
GTAAGACCAG TAGCATTGAT CCCAAGTTCC ATCCCCCATG AAGTGCCTCA GATCTTAATT 
AATAGGGAAC CTTTGCCTCA TCTACACTTT GACGTGGAGC TTCTCGGAGA CTGTGATGTT 
ATTATTAATG AATTATGTCA GAGGTTAGGT AGCGAATACA CAAAACTTTG CTACAACTCA 
GTAAAACTTT CAGAAATCAC AGAAAAGCCT CCACGGCCGC ACAAGGAGCT CGAAGTGCTC 
TCAGCTGAGC TCCCACCAAC CCCTCTGAAC ATTTCAGAAG GCTCCAGTTC ACCTGAAAGA 
ATGACCCCAC CTGATACTGC AGTGGTGTCT TCAGAACACC CACCTGAATG TAAGGTAGAA 
AACTGTGAGC CTGCCTCAGA GACTAAAGGG ACCTGCTCAG AGGACACGCT TCAGGACACA 
GAGCTGGCAT CAGAAAACCC TGAAAATCCT GCTGGTGAGC TAATGAACTC TGAAACAATG 
AAGGAAAATG GATCTAATGA TGGAGAAAAT AAAGAAAAGA GTGAAATACT GAAGAAGTGC 
TGGGTAAACA GATCCGCGAA AGAACAGATT AGCAAAAGGC TGGATGGTAC TCAGTATCTG 
TTTTTGCCAC CCAATCGCTA TATCTTCCAT GGTGCTGAGG TTTACTCGGA TTCTGAAGAC 
GATATCATAT CTTCCAGCTC TTGTGGCAGC AGTAGTGAGA GCGGCTCGTG CCGCAGTCAG 
AGCTTAGATG TAGAGGACGA GAGTGAGATG GAGGAGTTTT ACAATGGCAT AGATGAGGAT 
GCTCCCGAGC AGGAAGAGGA GCCTGGATTC GGAGAGGATG GAGCCGAACG GGAGGAATGG 
GCAGCTGAGG AATCAGCTGA TACAAATGAA GCTGCAGGGA CTGAACATCC GAGCAATGCG 
CTGTAA