RHOT1 cDNA ORF clone, Corvus brachyrhynchos(American crow)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOT1
Entrez Gene ID	103619082
Full Name	ras homolog family member T1
Gene Type	protein-coding
Organism	Corvus brachyrhynchos(American crow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008637564.2	XP_008635786.1	mitochondrial Rho GTPase 1

Pathways from BioSystems
Gene Ontology

The following RHOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo69448	XM_008637564.2 Latest version!	Corvus brachyrhynchos ras homolog family member T1 (RHOT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOT1 ( XM_008637564.2 ) cDNA ORF clone, Corvus brachyrhynchos(American crow) -> XP_008635786.1 Corvus brachyrhynchos ras homolog family member T1 (RHOT1), mRNA.

Price & Availability↑

CloneID	OCo69448
Clone ID Related Accession (Same CDS sequence)	XM_008637564.2
Accession Version	XM_008637564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-02
Organism	Corvus brachyrhynchos(American crow)
Product	mitochondrial Rho GTPase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008239056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 3, 2016 this sequence version replaced XM_008637564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Corvus brachyrhynchos Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTG TCAGTGAGGA ATTCCCAGAA GAGGTTCCAC CCCGAGCTGA AGAAATCACC 
ATTCCAGCTG ATGTCACTCC TGAAAGAGTG CCAACCCACA TAGTAGATTA TTCAGAAGCA 
GAGCAAAGTG ATGAGCAGCT TTACCATGAA ATATCACAGG CAAATGTGAT TTGTATAGTC 
TATGCTGTTA ATAACAAGAA TTCTATTGAT AAGGTAACGA GTCGATGGAT TCCTCTCATC 
AATGAAAGGA CAGACAAAGA TAGCAGGCTG CCTCTTATAT TAGTTGGAAA CAAGTCTGAT 
CTAGTGGAGT ATAGCAGTAT GGAAACTATC CTTCCCATTA TGAATCAATA TACAGAGATA 
GAAACGTGTG TAGAGTGTTC AGCCAAGAAC CTGAAGAATA TATCTGAACT ATTTTATTAT 
GCACAGAAAG CTGTTCTACA TCCTACAGGT CCTCTTTATT GCCCAGAGGA GAAAGAGATG 
AAACCTGCCT GTATTAAAGC ACTGACTCGC ATTTTTAGAA TTTCTGATCA AGATAATGAT 
GGTACTCTCA ATGATGCAGA GCTCAACTTC TTTCAGAGAA TTTGTTTTAA TACACCACTG 
GCACCTCAAG CTTTGGAAGA CGTAAAGAAT GTAGTCAGGA AAAACCTAAG TGATGGAGTT 
GCAGATAATG GATTGACATT AAAAGGTTTT CTTTTTCTAC ACACACTTTT CATTCAGCGA 
GGAAGGCATG AGACAACTTG GACTGTTTTG CGTCGCTTTG GCTATGACGA TGACTTAGAG 
CTTACCCCAG AGTACTTGTT CCCTCCCCTA AAAATTCCCC CTGACTGCAC AACAGAACTA 
AATCATCATG CATACTTGTT TCTTCAAAGT ATTTTTGATA AACATGATTT GGATAGAGAT 
TGTGCTTTAT CTCCTGATGA ATTGAAAGAC TTGTTCAAAG TCTTCCCTTA CATGCCATGG 
GGACCTGATG TGAACAACAC AGTTTGTACA AATGAAAGAG GATGGATCAC ATACCAAGGG 
TTTCTCTCGC AGTGGACACT AACCACATAC CTGGATGTTC AGCGTTGCCT GGAGTACCTG 
GGTTATTTAG GGTACTCCAT ACTTGCAGAA CAAGAATCTC AAGCATCAGC AATTACAGTG 
ACAAGAGATA AAAAGATAGA CCTCCAGAAA AAACAGACTC AGCGAAATGT CTTCCGATGC 
AATGTTGTTG GAATGAAAGG CTGTGGGAAA AGTGGAGTTC TTCAAGCTCT CCTTGGAAGA 
AATCTGATAA GACAGAGGCA AATACGTGCA GAACACAAAT CTTACTATGC CATTAATACA 
GTCTATGTAT ATGGACAGGA AAAATACTTG CTGCTACATG ATGCTGAGAC CATATGTGAT 
GCTGTCTGCC TGGTATATGA TGTCAGTAAT CCTAAATCCT TTGAATACTG TGCCAGGATT 
TTTAAGCAGC ACTTCATGGA CAGCAGAATA CCATGTTTAG TGGTAGCTGC CAAGTCCGAC 
TTGCATGAAG TTAGACAGGA ATATAGCATT TCTCCTGGTG AATTCTGCAA AAAACACAAA 
ATGCCTCCAC CCCAAGCCTT TACTTGTAAT ACTGCTGATG TGCCGAGTAA GGATATCTTT 
GTTAAGCTGA CAACTATGGC AATGTATCCC CATGCCCGGT TACGCTGTAT GTGCGCCTGC 
AACAGGTGTA CATTTTGCAT CTGTCAGAAC TTCCTCAACT CAGACTTGCT GCAATCTGTA 
AAGAACAAAC TCTTCACTGC AGTTCTTAAC AGGCATGTGA CACAAGCAGA CCTCAAGACC 
TCAACATTCT GGCTCCGAGC AAGTTTTGGT GCTACTGTGT TTGCAGTGTT GGGTTTTGCC 
ATGTACAAAG CACTACTAAA GCAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008635786.1
CDS	51..1940
Translation	MSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYHEISQANVICIVYAVNNKNSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFRISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKNLSDGVADNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTPEYLFPPLKIPPDCTTELNHHAYLFLQSIFDKHDLDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILAEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVVGMKGCGKSGVLQALLGRNLIRQRQIRAEHKSYYAINTVYVYGQEKYLLLHDAETICDAVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLVVAAKSDLHEVRQEYSISPGEFCKKHKMPPPQAFTCNTADVPSKDIFVKLTTMAMYPHARLRCMCACNRCTFCICQNFLNSDLLQSVKNKLFTAVLNRHVTQADLKTSTFWLRASFGATVFAVLGFAMYKALLKQR

Target ORF information:

RefSeq Version	XM_008637564.2
Organism	Corvus brachyrhynchos(American crow)
Definition	Corvus brachyrhynchos ras homolog family member T1 (RHOT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008637564.2

ORF Insert Sequence:

ATGTCTCTTG TCAGTGAGGA ATTCCCAGAA GAGGTTCCAC CCCGAGCTGA AGAAATCACC 
ATTCCAGCTG ATGTCACTCC TGAAAGAGTG CCAACCCACA TAGTAGATTA TTCAGAAGCA 
GAGCAAAGTG ATGAGCAGCT TTACCATGAA ATATCACAGG CAAATGTGAT TTGTATAGTC 
TATGCTGTTA ATAACAAGAA TTCTATTGAT AAGGTAACGA GTCGATGGAT TCCTCTCATC 
AATGAAAGGA CAGACAAAGA TAGCAGGCTG CCTCTTATAT TAGTTGGAAA CAAGTCTGAT 
CTAGTGGAGT ATAGCAGTAT GGAAACTATC CTTCCCATTA TGAATCAATA TACAGAGATA 
GAAACGTGTG TAGAGTGTTC AGCCAAGAAC CTGAAGAATA TATCTGAACT ATTTTATTAT 
GCACAGAAAG CTGTTCTACA TCCTACAGGT CCTCTTTATT GCCCAGAGGA GAAAGAGATG 
AAACCTGCCT GTATTAAAGC ACTGACTCGC ATTTTTAGAA TTTCTGATCA AGATAATGAT 
GGTACTCTCA ATGATGCAGA GCTCAACTTC TTTCAGAGAA TTTGTTTTAA TACACCACTG 
GCACCTCAAG CTTTGGAAGA CGTAAAGAAT GTAGTCAGGA AAAACCTAAG TGATGGAGTT 
GCAGATAATG GATTGACATT AAAAGGTTTT CTTTTTCTAC ACACACTTTT CATTCAGCGA 
GGAAGGCATG AGACAACTTG GACTGTTTTG CGTCGCTTTG GCTATGACGA TGACTTAGAG 
CTTACCCCAG AGTACTTGTT CCCTCCCCTA AAAATTCCCC CTGACTGCAC AACAGAACTA 
AATCATCATG CATACTTGTT TCTTCAAAGT ATTTTTGATA AACATGATTT GGATAGAGAT 
TGTGCTTTAT CTCCTGATGA ATTGAAAGAC TTGTTCAAAG TCTTCCCTTA CATGCCATGG 
GGACCTGATG TGAACAACAC AGTTTGTACA AATGAAAGAG GATGGATCAC ATACCAAGGG 
TTTCTCTCGC AGTGGACACT AACCACATAC CTGGATGTTC AGCGTTGCCT GGAGTACCTG 
GGTTATTTAG GGTACTCCAT ACTTGCAGAA CAAGAATCTC AAGCATCAGC AATTACAGTG 
ACAAGAGATA AAAAGATAGA CCTCCAGAAA AAACAGACTC AGCGAAATGT CTTCCGATGC 
AATGTTGTTG GAATGAAAGG CTGTGGGAAA AGTGGAGTTC TTCAAGCTCT CCTTGGAAGA 
AATCTGATAA GACAGAGGCA AATACGTGCA GAACACAAAT CTTACTATGC CATTAATACA 
GTCTATGTAT ATGGACAGGA AAAATACTTG CTGCTACATG ATGCTGAGAC CATATGTGAT 
GCTGTCTGCC TGGTATATGA TGTCAGTAAT CCTAAATCCT TTGAATACTG TGCCAGGATT 
TTTAAGCAGC ACTTCATGGA CAGCAGAATA CCATGTTTAG TGGTAGCTGC CAAGTCCGAC 
TTGCATGAAG TTAGACAGGA ATATAGCATT TCTCCTGGTG AATTCTGCAA AAAACACAAA 
ATGCCTCCAC CCCAAGCCTT TACTTGTAAT ACTGCTGATG TGCCGAGTAA GGATATCTTT 
GTTAAGCTGA CAACTATGGC AATGTATCCC CATGCCCGGT TACGCTGTAT GTGCGCCTGC 
AACAGGTGTA CATTTTGCAT CTGTCAGAAC TTCCTCAACT CAGACTTGCT GCAATCTGTA 
AAGAACAAAC TCTTCACTGC AGTTCTTAAC AGGCATGTGA CACAAGCAGA CCTCAAGACC 
TCAACATTCT GGCTCCGAGC AAGTTTTGGT GCTACTGTGT TTGCAGTGTT GGGTTTTGCC 
ATGTACAAAG CACTACTAAA GCAGCGATGA