RFFL cDNA ORF clone, Condylura cristata(star-nosed mole)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFFL
Entrez Gene ID	101628702
Full Name	ring finger and FYVE like domain containing E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Condylura cristata(star-nosed mole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004684905.2	XP_004684962.1	E3 ubiquitin-protein ligase rififylin isoform X1
XM_012726377.1	XP_012581831.1	E3 ubiquitin-protein ligase rififylin isoform X2

Pathways from BioSystems
Gene Ontology

The following RFFL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFFL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo235408	XM_004684905.2 Latest version!	Condylura cristata ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCo235409	XM_012726377.1 Latest version!	Condylura cristata ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFFL ( XM_004684905.2 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_004684962.1 Condylura cristata ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCo235408
Clone ID Related Accession (Same CDS sequence)	XM_004684905.2
Accession Version	XM_004684905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	E3 ubiquitin-protein ligase rififylin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567118.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2015 this sequence version replaced XM_004684905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAA CCTGCTGCAA CTGGTTTTGC CTGGATGGAC AGCCTGAGGA GGCCCCACTG 
CCCCAGGGAG CCAGGACACA AGCCTATTCC AACCCTGGGT ACAGCTCTTT CCCTTCTCCA 
CCAGGCTCAG AACCAAGCTG CAAGGCCTGT GGAGCCCACT TTGTAAACAT GGCCAGGAAG 
CAGACCTGCC TGGACTGTAA GAAAAATTTC TGCATGATCT GTTCGAACCA AGTGGGGAAT 
GGGCCCCGCC TCTGCCTTCT CTGCCAACGA TTCCGAGCCA CAGCCTTTCA GCGAGAGGAG 
CTCATGAAGA TGAAGGTGAA GGACCTGCGG GACTATCTGA GCCTCCATGA CATCTCTACA 
GACATGTGCC GGGAAAAAGA AGAGTTGGTG CTCTTGGTGC TTGGCCAGCA GCCTGTCACC 
TCCCACGAGG GCAGGACTCG TGCCTCCACC TTGTCCCCGG ACTTCCCAGA ACAGCAGGCC 
TTCCTGACGC AGCCGCACAC CAGCACAGCG CCTCCTACCT CACCCAACCT CCCCTCCTCA 
CCGGCACAGG CCACCTCTGG CCCCCAGGCC CGGGCTCAGG AGAATCAGCA GGCCAATGGC 
CATGTGTCTC AGGACCAAGA GGAACCCGTC TACTTGGAGA GCACGGCCAG AGCACCTGCT 
GAGGATGAGA CCCAGTCTCT CGACTCAGAG GATAGCTTCG TCCCGGGCCG GAGGGCCTCT 
CTGTCTGACC TGACTGACCT AGAGGACATT GAAGGCCTGA CTGTGCGGCA GCTGAAGGAG 
ATTCTGGCTC GCAATTTCGT CAACTACAAG GGCTGCTGTG AGAAGTGGGA GCTGATGGAG 
CGGGTGACGC GGCTGTACAA AGATCAGAAA GGACTCCAGC ACCTGGTGTG TGGTGCTGAA 
GACCAAAATG GGGGAGCAGT GCCACCTACT GTGGAGGATA ACCTGTGTAG GATCTGTATG 
GACTCACCCA TTGACTGTGT TCTGCTGGAG TGTGGCCACA TGGTAACCTG TACCAAGTGT 
GGAAAGCGCA TGAGTGAGTG TCCCATCTGT CGGCAATACG TGATTCGAGC TGTGCACGTC 
TTCCGATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004684962.1
CDS	9..1100
Translation	MWATCCNWFCLDGQPEEAPLPQGARTQAYSNPGYSSFPSPPGSEPSCKACGAHFVNMARKQTCLDCKKNFCMICSNQVGNGPRLCLLCQRFRATAFQREELMKMKVKDLRDYLSLHDISTDMCREKEELVLLVLGQQPVTSHEGRTRASTLSPDFPEQQAFLTQPHTSTAPPTSPNLPSSPAQATSGPQARAQENQQANGHVSQDQEEPVYLESTARAPAEDETQSLDSEDSFVPGRRASLSDLTDLEDIEGLTVRQLKEILARNFVNYKGCCEKWELMERVTRLYKDQKGLQHLVCGAEDQNGGAVPPTVEDNLCRICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVIRAVHVFRS

Target ORF information:

RefSeq Version	XM_004684905.2
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004684905.2

ORF Insert Sequence:

ATGTGGGCAA CCTGCTGCAA CTGGTTTTGC CTGGATGGAC AGCCTGAGGA GGCCCCACTG 
CCCCAGGGAG CCAGGACACA AGCCTATTCC AACCCTGGGT ACAGCTCTTT CCCTTCTCCA 
CCAGGCTCAG AACCAAGCTG CAAGGCCTGT GGAGCCCACT TTGTAAACAT GGCCAGGAAG 
CAGACCTGCC TGGACTGTAA GAAAAATTTC TGCATGATCT GTTCGAACCA AGTGGGGAAT 
GGGCCCCGCC TCTGCCTTCT CTGCCAACGA TTCCGAGCCA CAGCCTTTCA GCGAGAGGAG 
CTCATGAAGA TGAAGGTGAA GGACCTGCGG GACTATCTGA GCCTCCATGA CATCTCTACA 
GACATGTGCC GGGAAAAAGA AGAGTTGGTG CTCTTGGTGC TTGGCCAGCA GCCTGTCACC 
TCCCACGAGG GCAGGACTCG TGCCTCCACC TTGTCCCCGG ACTTCCCAGA ACAGCAGGCC 
TTCCTGACGC AGCCGCACAC CAGCACAGCG CCTCCTACCT CACCCAACCT CCCCTCCTCA 
CCGGCACAGG CCACCTCTGG CCCCCAGGCC CGGGCTCAGG AGAATCAGCA GGCCAATGGC 
CATGTGTCTC AGGACCAAGA GGAACCCGTC TACTTGGAGA GCACGGCCAG AGCACCTGCT 
GAGGATGAGA CCCAGTCTCT CGACTCAGAG GATAGCTTCG TCCCGGGCCG GAGGGCCTCT 
CTGTCTGACC TGACTGACCT AGAGGACATT GAAGGCCTGA CTGTGCGGCA GCTGAAGGAG 
ATTCTGGCTC GCAATTTCGT CAACTACAAG GGCTGCTGTG AGAAGTGGGA GCTGATGGAG 
CGGGTGACGC GGCTGTACAA AGATCAGAAA GGACTCCAGC ACCTGGTGTG TGGTGCTGAA 
GACCAAAATG GGGGAGCAGT GCCACCTACT GTGGAGGATA ACCTGTGTAG GATCTGTATG 
GACTCACCCA TTGACTGTGT TCTGCTGGAG TGTGGCCACA TGGTAACCTG TACCAAGTGT 
GGAAAGCGCA TGAGTGAGTG TCCCATCTGT CGGCAATACG TGATTCGAGC TGTGCACGTC 
TTCCGATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFFL ( XM_012726377.1 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_012581831.1 Condylura cristata ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCo235409
Clone ID Related Accession (Same CDS sequence)	XM_012726377.1
Accession Version	XM_012726377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	E3 ubiquitin-protein ligase rififylin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCAA CCTGCTGCAA CTGGTTTTGC CTGGATGGAC AGCCTGAGGA GGCCCCACTG 
CCCCAGGGAG CCAGGACACA AGCCTATTCC AACCCTGGGT ACAGCTCTTT CCCTTCTCCA 
CCAGGCTCAG AACCAAGCTG CAAGGCCTGT GGAGCCCACT TTGTAAACAT GGCCAGGAAG 
CAGACCTGCC TGGACTGTAA GAAAAATTTC TGCATGATCT GTTCGAACCA AGTGGGGAAT 
GGGCCCCGCC TCTGCCTTCT CTGCCAACGA TTCCGAGCCA CAGCCTTTCA GCGAGAGGAG 
CTCATGAAGA TGAAGGTGAA GGACCTGCGG GACTATCTGA GCCTCCATGA CATCTCTACA 
GACATGTGCC GGGAAAAAGA AGAGTTGGTG CTCTTGGTGC TTGGCCAGCA GCCTGTCACC 
TCCCACGAGG GCAGGACTCG TGCCTCCACC TTGTCCCCGG ACTTCCCAGA ACAGCAGGCC 
TTCCTGACGC AGCCGCACAC CAGCACAGCG CCTCCTACCT CACCCAACCT CCCCTCCTCA 
CCGGCACAGG CCACCTCTGG CCCCCAGGCC CGGGCTCAGG AGAATCAGCA GTCTCTCGAC 
TCAGAGGATA GCTTCGTCCC GGGCCGGAGG GCCTCTCTGT CTGACCTGAC TGACCTAGAG 
GACATTGAAG GCCTGACTGT GCGGCAGCTG AAGGAGATTC TGGCTCGCAA TTTCGTCAAC 
TACAAGGGCT GCTGTGAGAA GTGGGAGCTG ATGGAGCGGG TGACGCGGCT GTACAAAGAT 
CAGAAAGGAC TCCAGCACCT GGTGTGTGGT GCTGAAGACC AAAATGGGGG AGCAGTGCCA 
CCTACTGTGG AGGATAACCT GTGTAGGATC TGTATGGACT CACCCATTGA CTGTGTTCTG 
CTGGAGTGTG GCCACATGGT AACCTGTACC AAGTGTGGAA AGCGCATGAG TGAGTGTCCC 
ATCTGTCGGC AATACGTGAT TCGAGCTGTG CACGTCTTCC GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012581831.1
CDS	7..1014
Translation	MWATCCNWFCLDGQPEEAPLPQGARTQAYSNPGYSSFPSPPGSEPSCKACGAHFVNMARKQTCLDCKKNFCMICSNQVGNGPRLCLLCQRFRATAFQREELMKMKVKDLRDYLSLHDISTDMCREKEELVLLVLGQQPVTSHEGRTRASTLSPDFPEQQAFLTQPHTSTAPPTSPNLPSSPAQATSGPQARAQENQQSLDSEDSFVPGRRASLSDLTDLEDIEGLTVRQLKEILARNFVNYKGCCEKWELMERVTRLYKDQKGLQHLVCGAEDQNGGAVPPTVEDNLCRICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVIRAVHVFRS

Target ORF information:

RefSeq Version	XM_012726377.1
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012726377.1

ORF Insert Sequence:

ATGTGGGCAA CCTGCTGCAA CTGGTTTTGC CTGGATGGAC AGCCTGAGGA GGCCCCACTG 
CCCCAGGGAG CCAGGACACA AGCCTATTCC AACCCTGGGT ACAGCTCTTT CCCTTCTCCA 
CCAGGCTCAG AACCAAGCTG CAAGGCCTGT GGAGCCCACT TTGTAAACAT GGCCAGGAAG 
CAGACCTGCC TGGACTGTAA GAAAAATTTC TGCATGATCT GTTCGAACCA AGTGGGGAAT 
GGGCCCCGCC TCTGCCTTCT CTGCCAACGA TTCCGAGCCA CAGCCTTTCA GCGAGAGGAG 
CTCATGAAGA TGAAGGTGAA GGACCTGCGG GACTATCTGA GCCTCCATGA CATCTCTACA 
GACATGTGCC GGGAAAAAGA AGAGTTGGTG CTCTTGGTGC TTGGCCAGCA GCCTGTCACC 
TCCCACGAGG GCAGGACTCG TGCCTCCACC TTGTCCCCGG ACTTCCCAGA ACAGCAGGCC 
TTCCTGACGC AGCCGCACAC CAGCACAGCG CCTCCTACCT CACCCAACCT CCCCTCCTCA 
CCGGCACAGG CCACCTCTGG CCCCCAGGCC CGGGCTCAGG AGAATCAGCA GTCTCTCGAC 
TCAGAGGATA GCTTCGTCCC GGGCCGGAGG GCCTCTCTGT CTGACCTGAC TGACCTAGAG 
GACATTGAAG GCCTGACTGT GCGGCAGCTG AAGGAGATTC TGGCTCGCAA TTTCGTCAAC 
TACAAGGGCT GCTGTGAGAA GTGGGAGCTG ATGGAGCGGG TGACGCGGCT GTACAAAGAT 
CAGAAAGGAC TCCAGCACCT GGTGTGTGGT GCTGAAGACC AAAATGGGGG AGCAGTGCCA 
CCTACTGTGG AGGATAACCT GTGTAGGATC TGTATGGACT CACCCATTGA CTGTGTTCTG 
CTGGAGTGTG GCCACATGGT AACCTGTACC AAGTGTGGAA AGCGCATGAG TGAGTGTCCC 
ATCTGTCGGC AATACGTGAT TCGAGCTGTG CACGTCTTCC GATCCTGA