Sema5a cDNA ORF clone, Chinchilla lanigera(long-tailed chinchilla)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema5a
Entrez Gene ID	102019086
Full Name	semaphorin 5A
Gene Type	protein-coding
Organism	Chinchilla lanigera(long-tailed chinchilla)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005407077.2	XP_005407134.1	semaphorin-5A isoform X1
XM_013505908.1	XP_013361362.1	semaphorin-5A isoform X2

Pathways from BioSystems
Gene Ontology

The following Sema5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh229288	XM_005407077.2 Latest version!	Chinchilla lanigera sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCh229289	XM_013505908.1 Latest version!	Chinchilla lanigera sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema5a ( XM_005407077.2 ) cDNA ORF clone, Chinchilla lanigera(long-tailed chinchilla) -> XP_005407134.1 Chinchilla lanigera sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh229288
Clone ID Related Accession (Same CDS sequence)	XM_005407077.2
Accession Version	XM_005407077.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-18
Organism	Chinchilla lanigera(long-tailed chinchilla)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004955504.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 19, 2015 this sequence version replaced XM_005407077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chinchilla lanigera Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG CCTGCATCCT GGCATGGCTG TTCTCAAGCC TGGGGCTATG GAGACTCGTC 
CAGCTGGAGG CCCAGGATGC CCAGTGCCAG AGAGCCGAGC ACCCGGTCAT TTCCTACAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGACC CAGGACAGAA AGAGCTTGTG GTAGGAGCAA GAAACTACCT CTTCAGATTG 
CAACTTGAGG ATCTGTCTCT CATCCAGGCT GTGGAGTGGG AGTGTGACGA GGCGACCAAG 
AAGGCCTGTT ACAGCAAGGG CAAGTCAAAG GAGGAGTGCC AGAACTACAT CCGGGTGCTT 
TTGGTGGGTG GCGACCGGTT ATTCACCTGT GGGACCAACG CTTTCACACC TGTGTGCACC 
AACCGCTCGC TGAGTAACCT CACTGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT 
CCCTACAGTC CCCAGCACAA TTCCACAGCC CTCCTCACAG CCAGCGGGGA GCTCTATGCT 
GCAACAGCCA TGGACTTTCC AGGGCGAGAC CCTGCCATTT ACCGAAGTCT GGGCATTTTA 
CCCCCGCTCC GCACCGCGCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCC 
TCATATGACA TCGGAAACTT CACCTACTTC TTTTTCCGAG AAAATGCTGT GGAGCATGAC 
TGTGGGAAGA CCGTGTTCTC CAGGGCTGCA CGGGTCTGCA AGAACGACAT CGGGGGACGC 
TTCCTGCTGG AGGACACCTG GACCACCTTC ATGAAGGCGC GCCTGAACTG CTCCCGGCCC 
GGGGAGGTCC CCTTCTACTA CAACGAGCTG CAGAGCACTT TCTTCCTGCC CGAGCTGGAC 
TTGATCTATG GCATCTTCAC CACCAATGTG AACAGCATCG CCGCCTCCGC TGTGTGTGTC 
TTCAACCTGA GCGCCATCTC TCAGGCCTTC AATGGACCCT TCAAGTTCCA GGAGAACTCT 
CGCTCAGCCT GGCTACCTTA TCCCAACCCC AACCCCCACT TCCAGTGTGG CACCGTGGAC 
CAGGGCCTGT ACGTGAACCT AACGGAAAGG AACCTGCAGG ATGCTCAGAA GTTCATCCTG 
ATGCACGAGG TGGTGCAGCC GGTGACCACA GCGCCGTCCT TCCTGGAGGA CAACAGCCGC 
TTCTCCCACG TGGTGGTGGA CGTGGTGCAG GGTAGAGACG CACTCGTCCA CATCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAA GTACGGGCAC CTCTGAATGA GACCTCAAGC 
AGCTGTTTAC TGGAAGAGAT TGAGCTCTTC CCAGAGAGGA GGAGGGAGCC CATCCGGAGC 
CTGCAGATCC TCCACAGCCA GAGCGTCCTG TTTGTGGGGC TGCGGGAGCA CGTGGTCAAG 
ATCCCCCTGA AGCGGTGTCA GTTCTTCCAC ACGCGCAGCT CCTGCATCGG GGCCCAGGAC 
CCTTACTGTG GCTGGGACGT CGTGATGAAC AAGTGCACCA GCCTGGAGGA GAGCCTCAGC 
ATGACGCAGT GGGAACAGAG CCTCTCAGGC TGCCCGACCA GGAATCTCAC TGTGGATGGG 
CGCTTTGGCC CGTGGTCTCC GTGGACGCTC TGCACACACA CAGACGGCAG TGCCGTGGGA 
TCCTGCCTCT GTCGGTCCCG CTCCTGTGAC AGCCCAGCCC CACAATGTGG CGGCTGGCAG 
TGTGAGGGCC CCAGCATGGA GATTGCCAAC TGCTCCAGGA ATGGAGGCTG GACCCCCTGG 
ACCTCCTGGT CGCCCTGCAG CACCACCTGT GGCATTGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACGCCAAG GCATGGCGGC CGGGTGTGCG TGGGACAGAA CCGCGAGGAA 
AGATACTGCA ATGAACATCT GCTCTGCCCA CCGCACGTGT TCTGGACAGG CTGGGGGCCT 
TGGGAGAGGT GCACTGCCCA GTGTGGGGGT GGCATTCAGG CTCGCCGCAG GACCTGTGAG 
AATGGGGACG ACTGTATAGG CTGCAACGTG GAATACCAGC CTTGTAACAC CAACCCATGT 
CCTGAGCTAA AGAAGACCAC ACCCTGGACA CCGTGGACGC CCGTCAACAT CTCTGACAAT 
GGCGGCCACT ATGAGCAGCG CTTCCGGTAC ACCTGCAAGG CCCGCCTGCC AGATCCCAAT 
CTGCTAGAAG TGGGAAGGCA GAGAATTGAA ATGCGGTACT GTTCTAGTGA TGGAACCAGC 
GGCTGCTCCA CAGATGGACT TTCTGGGGAC TTCCTGCGCG CCGGGAGGTA CTCAGCCCAT 
GCTGTCAACG GGGGCTGGTC AGCCTGGACA CCCTGGTCCA AGTGCAGCCG GGACTGCGGC 
AGGGGCATTC GGAGCCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTA TGGAGGGGTG 
CCGTGCCTGG GCCCGTCCCT GGAGTACCAG GAGTGCAACA TTTTGCCCTG CCCAGTGGAT 
GGCGGGTGGT CTTGCTGGTC GTCCTGGTCA CAGTGCTCAG CAACGTGTGG CGGCGGACAC 
CACATGAGGA CGCGCTCGTG CACAAACCCC GCCCCAGCCT ATGGAGGGGA CATCTGCCTG 
GGGCTGCACA CCGAAGAGGC TCTCTGCAAC ACACAGCCCT GCCCAGAGAG CTGGTCAGAG 
TGGTCCGACT GGTCTGTGTG CGACCCATCT GGCGTCCAGA TCCGCGTCCG CCATTGCCTC 
CTTCCGTTCC CGGTGGGCAG CCAGTGCTCC GGGAACAACA CAGAGAGCCG GCCGTGCGTT 
TTTGACTCCA ACTTCATCCC AGAAGTATCT GTGGCGAGAT CCAGTAGTGT AGAAGAGAAA 
AGATGTGGAG AGTTCAACAT GTTCCACATG ATTGCCGTGG GCCTGAGCAG CTCCATCCTC 
GGCTGCCTCC TCACCCTGCT TGTCTACACC TACTGCCAGC GCTACCAGCA GCAATCCCAC 
GACGCGACTG TCATCCACCC CGTGTCGCCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATCAACAAAC TGGACAAGTA CGACTCAGTG GAGGCTATCA AGGCATTTAA CAAAAACAAC 
CTGATCCTAG AGGAAAGAAA CAAGTACTTC AACCCTCATC TCACTGGGAA GACCTATTCT 
AATGCCTACT TTACAGATCT CAATAACTAC GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005407134.1
CDS	170..3391
Translation	MKGACILAWLFSSLGLWRLVQLEAQDAQCQRAEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKFQENSRSAWLPYPNPNPHFQCGTVDQGLYVNLTERNLQDAQKFILMHEVVQPVTTAPSFLEDNSRFSHVVVDVVQGRDALVHIIYLATDYGTIKKVRAPLNETSSSCLLEEIELFPERRREPIRSLQILHSQSVLFVGLREHVVKIPLKRCQFFHTRSSCIGAQDPYCGWDVVMNKCTSLEESLSMTQWEQSLSGCPTRNLTVDGRFGPWSPWTLCTHTDGSAVGSCLCRSRSCDSPAPQCGGWQCEGPSMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGDDCIGCNVEYQPCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHAVNGGWSAWTPWSKCSRDCGRGIRSRKRVCNNPEPKYGGVPCLGPSLEYQECNILPCPVDGGWSCWSSWSQCSATCGGGHHMRTRSCTNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSDWSVCDPSGVQIRVRHCLLPFPVGSQCSGNNTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMIAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_005407077.2
Organism	Chinchilla lanigera(long-tailed chinchilla)
Definition	Chinchilla lanigera sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005407077.2

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT GGCATGGCTG TTCTCAAGCC TGGGGCTATG GAGACTCGTC 
CAGCTGGAGG CCCAGGATGC CCAGTGCCAG AGAGCCGAGC ACCCGGTCAT TTCCTACAAA 
GAAATTGGCC CCTGGTTGCG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA 
ACATTTGACC CAGGACAGAA AGAGCTTGTG GTAGGAGCAA GAAACTACCT CTTCAGATTG 
CAACTTGAGG ATCTGTCTCT CATCCAGGCT GTGGAGTGGG AGTGTGACGA GGCGACCAAG 
AAGGCCTGTT ACAGCAAGGG CAAGTCAAAG GAGGAGTGCC AGAACTACAT CCGGGTGCTT 
TTGGTGGGTG GCGACCGGTT ATTCACCTGT GGGACCAACG CTTTCACACC TGTGTGCACC 
AACCGCTCGC TGAGTAACCT CACTGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT 
CCCTACAGTC CCCAGCACAA TTCCACAGCC CTCCTCACAG CCAGCGGGGA GCTCTATGCT 
GCAACAGCCA TGGACTTTCC AGGGCGAGAC CCTGCCATTT ACCGAAGTCT GGGCATTTTA 
CCCCCGCTCC GCACCGCGCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCC 
TCATATGACA TCGGAAACTT CACCTACTTC TTTTTCCGAG AAAATGCTGT GGAGCATGAC 
TGTGGGAAGA CCGTGTTCTC CAGGGCTGCA CGGGTCTGCA AGAACGACAT CGGGGGACGC 
TTCCTGCTGG AGGACACCTG GACCACCTTC ATGAAGGCGC GCCTGAACTG CTCCCGGCCC 
GGGGAGGTCC CCTTCTACTA CAACGAGCTG CAGAGCACTT TCTTCCTGCC CGAGCTGGAC 
TTGATCTATG GCATCTTCAC CACCAATGTG AACAGCATCG CCGCCTCCGC TGTGTGTGTC 
TTCAACCTGA GCGCCATCTC TCAGGCCTTC AATGGACCCT TCAAGTTCCA GGAGAACTCT 
CGCTCAGCCT GGCTACCTTA TCCCAACCCC AACCCCCACT TCCAGTGTGG CACCGTGGAC 
CAGGGCCTGT ACGTGAACCT AACGGAAAGG AACCTGCAGG ATGCTCAGAA GTTCATCCTG 
ATGCACGAGG TGGTGCAGCC GGTGACCACA GCGCCGTCCT TCCTGGAGGA CAACAGCCGC 
TTCTCCCACG TGGTGGTGGA CGTGGTGCAG GGTAGAGACG CACTCGTCCA CATCATCTAC 
CTGGCCACAG ATTATGGCAC CATTAAGAAA GTACGGGCAC CTCTGAATGA GACCTCAAGC 
AGCTGTTTAC TGGAAGAGAT TGAGCTCTTC CCAGAGAGGA GGAGGGAGCC CATCCGGAGC 
CTGCAGATCC TCCACAGCCA GAGCGTCCTG TTTGTGGGGC TGCGGGAGCA CGTGGTCAAG 
ATCCCCCTGA AGCGGTGTCA GTTCTTCCAC ACGCGCAGCT CCTGCATCGG GGCCCAGGAC 
CCTTACTGTG GCTGGGACGT CGTGATGAAC AAGTGCACCA GCCTGGAGGA GAGCCTCAGC 
ATGACGCAGT GGGAACAGAG CCTCTCAGGC TGCCCGACCA GGAATCTCAC TGTGGATGGG 
CGCTTTGGCC CGTGGTCTCC GTGGACGCTC TGCACACACA CAGACGGCAG TGCCGTGGGA 
TCCTGCCTCT GTCGGTCCCG CTCCTGTGAC AGCCCAGCCC CACAATGTGG CGGCTGGCAG 
TGTGAGGGCC CCAGCATGGA GATTGCCAAC TGCTCCAGGA ATGGAGGCTG GACCCCCTGG 
ACCTCCTGGT CGCCCTGCAG CACCACCTGT GGCATTGGCT TCCAGGTGCG GCAGCGGTCC 
TGCAGCAACC CCACGCCAAG GCATGGCGGC CGGGTGTGCG TGGGACAGAA CCGCGAGGAA 
AGATACTGCA ATGAACATCT GCTCTGCCCA CCGCACGTGT TCTGGACAGG CTGGGGGCCT 
TGGGAGAGGT GCACTGCCCA GTGTGGGGGT GGCATTCAGG CTCGCCGCAG GACCTGTGAG 
AATGGGGACG ACTGTATAGG CTGCAACGTG GAATACCAGC CTTGTAACAC CAACCCATGT 
CCTGAGCTAA AGAAGACCAC ACCCTGGACA CCGTGGACGC CCGTCAACAT CTCTGACAAT 
GGCGGCCACT ATGAGCAGCG CTTCCGGTAC ACCTGCAAGG CCCGCCTGCC AGATCCCAAT 
CTGCTAGAAG TGGGAAGGCA GAGAATTGAA ATGCGGTACT GTTCTAGTGA TGGAACCAGC 
GGCTGCTCCA CAGATGGACT TTCTGGGGAC TTCCTGCGCG CCGGGAGGTA CTCAGCCCAT 
GCTGTCAACG GGGGCTGGTC AGCCTGGACA CCCTGGTCCA AGTGCAGCCG GGACTGCGGC 
AGGGGCATTC GGAGCCGGAA GCGTGTCTGC AACAACCCGG AGCCCAAGTA TGGAGGGGTG 
CCGTGCCTGG GCCCGTCCCT GGAGTACCAG GAGTGCAACA TTTTGCCCTG CCCAGTGGAT 
GGCGGGTGGT CTTGCTGGTC GTCCTGGTCA CAGTGCTCAG CAACGTGTGG CGGCGGACAC 
CACATGAGGA CGCGCTCGTG CACAAACCCC GCCCCAGCCT ATGGAGGGGA CATCTGCCTG 
GGGCTGCACA CCGAAGAGGC TCTCTGCAAC ACACAGCCCT GCCCAGAGAG CTGGTCAGAG 
TGGTCCGACT GGTCTGTGTG CGACCCATCT GGCGTCCAGA TCCGCGTCCG CCATTGCCTC 
CTTCCGTTCC CGGTGGGCAG CCAGTGCTCC GGGAACAACA CAGAGAGCCG GCCGTGCGTT 
TTTGACTCCA ACTTCATCCC AGAAGTATCT GTGGCGAGAT CCAGTAGTGT AGAAGAGAAA 
AGATGTGGAG AGTTCAACAT GTTCCACATG ATTGCCGTGG GCCTGAGCAG CTCCATCCTC 
GGCTGCCTCC TCACCCTGCT TGTCTACACC TACTGCCAGC GCTACCAGCA GCAATCCCAC 
GACGCGACTG TCATCCACCC CGTGTCGCCC GCCCCTCTCA ACACCAGCAT AACCAACCAC 
ATCAACAAAC TGGACAAGTA CGACTCAGTG GAGGCTATCA AGGCATTTAA CAAAAACAAC 
CTGATCCTAG AGGAAAGAAA CAAGTACTTC AACCCTCATC TCACTGGGAA GACCTATTCT 
AATGCCTACT TTACAGATCT CAATAACTAC GATGAGTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5a ( XM_013505908.1 ) cDNA ORF clone, Chinchilla lanigera(long-tailed chinchilla) -> XP_013361362.1 Chinchilla lanigera sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCh229289
Clone ID Related Accession (Same CDS sequence)	XM_013505908.1
Accession Version	XM_013505908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-18
Organism	Chinchilla lanigera(long-tailed chinchilla)
Product	semaphorin-5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004955504.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chinchilla lanigera Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTTC TCAAGCCTGG GGCTATGGAG ACTCGTCCAG CTGGAGGCCC AGGATGCCCA 
GTGCCAGAGA GCCGAGCACC CGGTCATTTC CTACAAAGAA ACTACCTCTT CAGATTGCAA 
CTTGAGGATC TGTCTCTCAT CCAGGCTGTG GAGTGGGAGT GTGACGAGGC GACCAAGAAG 
GCCTGTTACA GCAAGGGCAA GTCAAAGGAG GAGTGCCAGA ACTACATCCG GGTGCTTTTG 
GTGGGTGGCG ACCGGTTATT CACCTGTGGG ACCAACGCTT TCACACCTGT GTGCACCAAC 
CGCTCGCTGA GTAACCTCAC TGAGATCCAT GATCAGATCA GTGGCATGGC CCGCTGTCCC 
TACAGTCCCC AGCACAATTC CACAGCCCTC CTCACAGCCA GCGGGGAGCT CTATGCTGCA 
ACAGCCATGG ACTTTCCAGG GCGAGACCCT GCCATTTACC GAAGTCTGGG CATTTTACCC 
CCGCTCCGCA CCGCGCAGTA CAACTCCAAG TGGCTCAATG AACCAAACTT CGTGTCCTCA 
TATGACATCG GAAACTTCAC CTACTTCTTT TTCCGAGAAA ATGCTGTGGA GCATGACTGT 
GGGAAGACCG TGTTCTCCAG GGCTGCACGG GTCTGCAAGA ACGACATCGG GGGACGCTTC 
CTGCTGGAGG ACACCTGGAC CACCTTCATG AAGGCGCGCC TGAACTGCTC CCGGCCCGGG 
GAGGTCCCCT TCTACTACAA CGAGCTGCAG AGCACTTTCT TCCTGCCCGA GCTGGACTTG 
ATCTATGGCA TCTTCACCAC CAATGTGAAC AGCATCGCCG CCTCCGCTGT GTGTGTCTTC 
AACCTGAGCG CCATCTCTCA GGCCTTCAAT GGACCCTTCA AGTTCCAGGA GAACTCTCGC 
TCAGCCTGGC TACCTTATCC CAACCCCAAC CCCCACTTCC AGTGTGGCAC CGTGGACCAG 
GGCCTGTACG TGAACCTAAC GGAAAGGAAC CTGCAGGATG CTCAGAAGTT CATCCTGATG 
CACGAGGTGG TGCAGCCGGT GACCACAGCG CCGTCCTTCC TGGAGGACAA CAGCCGCTTC 
TCCCACGTGG TGGTGGACGT GGTGCAGGGT AGAGACGCAC TCGTCCACAT CATCTACCTG 
GCCACAGATT ATGGCACCAT TAAGAAAGTA CGGGCACCTC TGAATGAGAC CTCAAGCAGC 
TGTTTACTGG AAGAGATTGA GCTCTTCCCA GAGAGGAGGA GGGAGCCCAT CCGGAGCCTG 
CAGATCCTCC ACAGCCAGAG CGTCCTGTTT GTGGGGCTGC GGGAGCACGT GGTCAAGATC 
CCCCTGAAGC GGTGTCAGTT CTTCCACACG CGCAGCTCCT GCATCGGGGC CCAGGACCCT 
TACTGTGGCT GGGACGTCGT GATGAACAAG TGCACCAGCC TGGAGGAGAG CCTCAGCATG 
ACGCAGTGGG AACAGAGCCT CTCAGGCTGC CCGACCAGGA ATCTCACTGT GGATGGGCGC 
TTTGGCCCGT GGTCTCCGTG GACGCTCTGC ACACACACAG ACGGCAGTGC CGTGGGATCC 
TGCCTCTGTC GGTCCCGCTC CTGTGACAGC CCAGCCCCAC AATGTGGCGG CTGGCAGTGT 
GAGGGCCCCA GCATGGAGAT TGCCAACTGC TCCAGGAATG GAGGCTGGAC CCCCTGGACC 
TCCTGGTCGC CCTGCAGCAC CACCTGTGGC ATTGGCTTCC AGGTGCGGCA GCGGTCCTGC 
AGCAACCCCA CGCCAAGGCA TGGCGGCCGG GTGTGCGTGG GACAGAACCG CGAGGAAAGA 
TACTGCAATG AACATCTGCT CTGCCCACCG CACGTGTTCT GGACAGGCTG GGGGCCTTGG 
GAGAGGTGCA CTGCCCAGTG TGGGGGTGGC ATTCAGGCTC GCCGCAGGAC CTGTGAGAAT 
GGGGACGACT GTATAGGCTG CAACGTGGAA TACCAGCCTT GTAACACCAA CCCATGTCCT 
GAGCTAAAGA AGACCACACC CTGGACACCG TGGACGCCCG TCAACATCTC TGACAATGGC 
GGCCACTATG AGCAGCGCTT CCGGTACACC TGCAAGGCCC GCCTGCCAGA TCCCAATCTG 
CTAGAAGTGG GAAGGCAGAG AATTGAAATG CGGTACTGTT CTAGTGATGG AACCAGCGGC 
TGCTCCACAG ATGGACTTTC TGGGGACTTC CTGCGCGCCG GGAGGTACTC AGCCCATGCT 
GTCAACGGGG GCTGGTCAGC CTGGACACCC TGGTCCAAGT GCAGCCGGGA CTGCGGCAGG 
GGCATTCGGA GCCGGAAGCG TGTCTGCAAC AACCCGGAGC CCAAGTATGG AGGGGTGCCG 
TGCCTGGGCC CGTCCCTGGA GTACCAGGAG TGCAACATTT TGCCCTGCCC AGTGGATGGC 
GGGTGGTCTT GCTGGTCGTC CTGGTCACAG TGCTCAGCAA CGTGTGGCGG CGGACACCAC 
ATGAGGACGC GCTCGTGCAC AAACCCCGCC CCAGCCTATG GAGGGGACAT CTGCCTGGGG 
CTGCACACCG AAGAGGCTCT CTGCAACACA CAGCCCTGCC CAGAGAGCTG GTCAGAGTGG 
TCCGACTGGT CTGTGTGCGA CCCATCTGGC GTCCAGATCC GCGTCCGCCA TTGCCTCCTT 
CCGTTCCCGG TGGGCAGCCA GTGCTCCGGG AACAACACAG AGAGCCGGCC GTGCGTTTTT 
GACTCCAACT TCATCCCAGA AGTATCTGTG GCGAGATCCA GTAGTGTAGA AGAGAAAAGA 
TGTGGAGAGT TCAACATGTT CCACATGATT GCCGTGGGCC TGAGCAGCTC CATCCTCGGC 
TGCCTCCTCA CCCTGCTTGT CTACACCTAC TGCCAGCGCT ACCAGCAGCA ATCCCACGAC 
GCGACTGTCA TCCACCCCGT GTCGCCCGCC CCTCTCAACA CCAGCATAAC CAACCACATC 
AACAAACTGG ACAAGTACGA CTCAGTGGAG GCTATCAAGG CATTTAACAA AAACAACCTG 
ATCCTAGAGG AAAGAAACAA GTACTTCAAC CCTCATCTCA CTGGGAAGAC CTATTCTAAT 
GCCTACTTTA CAGATCTCAA TAACTACGAT GAGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013361362.1
CDS	193..3291
Translation	MAVLKPGAMETRPAGGPGCPVPESRAPGHFLQRNYLFRLQLEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKFQENSRSAWLPYPNPNPHFQCGTVDQGLYVNLTERNLQDAQKFILMHEVVQPVTTAPSFLEDNSRFSHVVVDVVQGRDALVHIIYLATDYGTIKKVRAPLNETSSSCLLEEIELFPERRREPIRSLQILHSQSVLFVGLREHVVKIPLKRCQFFHTRSSCIGAQDPYCGWDVVMNKCTSLEESLSMTQWEQSLSGCPTRNLTVDGRFGPWSPWTLCTHTDGSAVGSCLCRSRSCDSPAPQCGGWQCEGPSMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGDDCIGCNVEYQPCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHAVNGGWSAWTPWSKCSRDCGRGIRSRKRVCNNPEPKYGGVPCLGPSLEYQECNILPCPVDGGWSCWSSWSQCSATCGGGHHMRTRSCTNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSDWSVCDPSGVQIRVRHCLLPFPVGSQCSGNNTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMIAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_013505908.1
Organism	Chinchilla lanigera(long-tailed chinchilla)
Definition	Chinchilla lanigera sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013505908.1

ORF Insert Sequence:

ATGGCTGTTC TCAAGCCTGG GGCTATGGAG ACTCGTCCAG CTGGAGGCCC AGGATGCCCA 
GTGCCAGAGA GCCGAGCACC CGGTCATTTC CTACAAAGAA ACTACCTCTT CAGATTGCAA 
CTTGAGGATC TGTCTCTCAT CCAGGCTGTG GAGTGGGAGT GTGACGAGGC GACCAAGAAG 
GCCTGTTACA GCAAGGGCAA GTCAAAGGAG GAGTGCCAGA ACTACATCCG GGTGCTTTTG 
GTGGGTGGCG ACCGGTTATT CACCTGTGGG ACCAACGCTT TCACACCTGT GTGCACCAAC 
CGCTCGCTGA GTAACCTCAC TGAGATCCAT GATCAGATCA GTGGCATGGC CCGCTGTCCC 
TACAGTCCCC AGCACAATTC CACAGCCCTC CTCACAGCCA GCGGGGAGCT CTATGCTGCA 
ACAGCCATGG ACTTTCCAGG GCGAGACCCT GCCATTTACC GAAGTCTGGG CATTTTACCC 
CCGCTCCGCA CCGCGCAGTA CAACTCCAAG TGGCTCAATG AACCAAACTT CGTGTCCTCA 
TATGACATCG GAAACTTCAC CTACTTCTTT TTCCGAGAAA ATGCTGTGGA GCATGACTGT 
GGGAAGACCG TGTTCTCCAG GGCTGCACGG GTCTGCAAGA ACGACATCGG GGGACGCTTC 
CTGCTGGAGG ACACCTGGAC CACCTTCATG AAGGCGCGCC TGAACTGCTC CCGGCCCGGG 
GAGGTCCCCT TCTACTACAA CGAGCTGCAG AGCACTTTCT TCCTGCCCGA GCTGGACTTG 
ATCTATGGCA TCTTCACCAC CAATGTGAAC AGCATCGCCG CCTCCGCTGT GTGTGTCTTC 
AACCTGAGCG CCATCTCTCA GGCCTTCAAT GGACCCTTCA AGTTCCAGGA GAACTCTCGC 
TCAGCCTGGC TACCTTATCC CAACCCCAAC CCCCACTTCC AGTGTGGCAC CGTGGACCAG 
GGCCTGTACG TGAACCTAAC GGAAAGGAAC CTGCAGGATG CTCAGAAGTT CATCCTGATG 
CACGAGGTGG TGCAGCCGGT GACCACAGCG CCGTCCTTCC TGGAGGACAA CAGCCGCTTC 
TCCCACGTGG TGGTGGACGT GGTGCAGGGT AGAGACGCAC TCGTCCACAT CATCTACCTG 
GCCACAGATT ATGGCACCAT TAAGAAAGTA CGGGCACCTC TGAATGAGAC CTCAAGCAGC 
TGTTTACTGG AAGAGATTGA GCTCTTCCCA GAGAGGAGGA GGGAGCCCAT CCGGAGCCTG 
CAGATCCTCC ACAGCCAGAG CGTCCTGTTT GTGGGGCTGC GGGAGCACGT GGTCAAGATC 
CCCCTGAAGC GGTGTCAGTT CTTCCACACG CGCAGCTCCT GCATCGGGGC CCAGGACCCT 
TACTGTGGCT GGGACGTCGT GATGAACAAG TGCACCAGCC TGGAGGAGAG CCTCAGCATG 
ACGCAGTGGG AACAGAGCCT CTCAGGCTGC CCGACCAGGA ATCTCACTGT GGATGGGCGC 
TTTGGCCCGT GGTCTCCGTG GACGCTCTGC ACACACACAG ACGGCAGTGC CGTGGGATCC 
TGCCTCTGTC GGTCCCGCTC CTGTGACAGC CCAGCCCCAC AATGTGGCGG CTGGCAGTGT 
GAGGGCCCCA GCATGGAGAT TGCCAACTGC TCCAGGAATG GAGGCTGGAC CCCCTGGACC 
TCCTGGTCGC CCTGCAGCAC CACCTGTGGC ATTGGCTTCC AGGTGCGGCA GCGGTCCTGC 
AGCAACCCCA CGCCAAGGCA TGGCGGCCGG GTGTGCGTGG GACAGAACCG CGAGGAAAGA 
TACTGCAATG AACATCTGCT CTGCCCACCG CACGTGTTCT GGACAGGCTG GGGGCCTTGG 
GAGAGGTGCA CTGCCCAGTG TGGGGGTGGC ATTCAGGCTC GCCGCAGGAC CTGTGAGAAT 
GGGGACGACT GTATAGGCTG CAACGTGGAA TACCAGCCTT GTAACACCAA CCCATGTCCT 
GAGCTAAAGA AGACCACACC CTGGACACCG TGGACGCCCG TCAACATCTC TGACAATGGC 
GGCCACTATG AGCAGCGCTT CCGGTACACC TGCAAGGCCC GCCTGCCAGA TCCCAATCTG 
CTAGAAGTGG GAAGGCAGAG AATTGAAATG CGGTACTGTT CTAGTGATGG AACCAGCGGC 
TGCTCCACAG ATGGACTTTC TGGGGACTTC CTGCGCGCCG GGAGGTACTC AGCCCATGCT 
GTCAACGGGG GCTGGTCAGC CTGGACACCC TGGTCCAAGT GCAGCCGGGA CTGCGGCAGG 
GGCATTCGGA GCCGGAAGCG TGTCTGCAAC AACCCGGAGC CCAAGTATGG AGGGGTGCCG 
TGCCTGGGCC CGTCCCTGGA GTACCAGGAG TGCAACATTT TGCCCTGCCC AGTGGATGGC 
GGGTGGTCTT GCTGGTCGTC CTGGTCACAG TGCTCAGCAA CGTGTGGCGG CGGACACCAC 
ATGAGGACGC GCTCGTGCAC AAACCCCGCC CCAGCCTATG GAGGGGACAT CTGCCTGGGG 
CTGCACACCG AAGAGGCTCT CTGCAACACA CAGCCCTGCC CAGAGAGCTG GTCAGAGTGG 
TCCGACTGGT CTGTGTGCGA CCCATCTGGC GTCCAGATCC GCGTCCGCCA TTGCCTCCTT 
CCGTTCCCGG TGGGCAGCCA GTGCTCCGGG AACAACACAG AGAGCCGGCC GTGCGTTTTT 
GACTCCAACT TCATCCCAGA AGTATCTGTG GCGAGATCCA GTAGTGTAGA AGAGAAAAGA 
TGTGGAGAGT TCAACATGTT CCACATGATT GCCGTGGGCC TGAGCAGCTC CATCCTCGGC 
TGCCTCCTCA CCCTGCTTGT CTACACCTAC TGCCAGCGCT ACCAGCAGCA ATCCCACGAC 
GCGACTGTCA TCCACCCCGT GTCGCCCGCC CCTCTCAACA CCAGCATAAC CAACCACATC 
AACAAACTGG ACAAGTACGA CTCAGTGGAG GCTATCAAGG CATTTAACAA AAACAACCTG 
ATCCTAGAGG AAAGAAACAA GTACTTCAAC CCTCATCTCA CTGGGAAGAC CTATTCTAAT 
GCCTACTTTA CAGATCTCAA TAACTACGAT GAGTACTAA