RPE65 cDNA ORF clone, Cercocebus atys(sooty mangabey)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPE65
Entrez Gene ID	105595883
Full Name	RPE65, retinoid isomerohydrolase
Gene Type	protein-coding
Organism	Cercocebus atys(sooty mangabey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012082287.1	XP_011937677.1	LOW QUALITY PROTEIN: retinoid isomerohydrolase

Pathways from BioSystems
Gene Ontology

The following RPE65 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPE65 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe221198	XM_012082287.1 Latest version!	Cercocebus atys retinal pigment epithelium-specific protein 65kDa (RPE65), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPE65 ( XM_012082287.1 ) cDNA ORF clone, Cercocebus atys(sooty mangabey) -> XP_011937677.1 Cercocebus atys retinal pigment epithelium-specific protein 65kDa (RPE65), mRNA.

Price & Availability↑

CloneID	OCe221198
Clone ID Related Accession (Same CDS sequence)	XM_012082287.1
Accession Version	XM_012082287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-29
Organism	Cercocebus atys(sooty mangabey)
Product	LOW QUALITY PROTEIN: retinoid isomerohydrolase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012001850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cercocebus atys Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTCTATCC AGGTCGAGCA TCCTGCTGGT GGTTACAAGA AGCTGTTTGA AACTGTGGAG 
GAACTGTCCT CGCCGCTCAC GGCTCATGTA ACAGGCAGGA TCCCCCTGTG GCTCACCGGC 
AGTCTCCTCC GATGTGGGCC AGGACTCTTT GAAGTTGGAT CTGAGCCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT GCACAAATTT GACTTTAAAG AAGGACACGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTTC GAGGAGTGGA GGTTACCGAC AACGCCCTTG TTAATGTCTA CCCAGTGGGG 
GAAGACTACT ACGCTTGCAC AGAGACCAAC TTTATTACAA AGATTAATCC AGAGACCTTG 
GAGACAATTA AGCAGGTTGA TCTTTGCAAC TACGTCTCCG TCAATGGAGC CACTGCTCAC 
CCCCACATTG AAAATGATGG AACCGTTTAC AATATTGGTA ATTGCTTTGG AAAAAATTTT 
TCAATTGCCT ACAACATTGT AAAGATCCCA CCACTGCAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTT 
CATAGTTTTG GTTTGACTCC CAACTATATC GTTTTTGTGG AGACACCAGT CAAAATTAAC 
CTGTTCAAGT TCCTTTCTTC ATGGAGTCTT TGGGGAGCCA ACTACATGGA TTGCTTTGAG 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGGAA AAAGTACCTC 
AATAATAAAT ACAGGACTTC TCCTTTCAAC CTCTTCCATC ACATCAACAC CTATGAAGAC 
AATGGGTTTC TGATTGTGGA TCTCTGCTGC TGGAAAGGAT TTGAGTTTGT TTATAATTAC 
TTATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCCCAACCTG AAGTTAGGAG ATATGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAA TCACGCTCCC CAATACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACA 
ATCTGGCTGG AGCCTGAGGT TCTCTTTTCA GGGCCTCGCC AAGCGTTTGA GTTTCCTCAA 
ATCNNATACC AGAAGTATTG TGGGAAACCT TACACATACG CGTATGGACT TGGCTTGAAT 
CACTTTGTTC CAGATAGGCT CTGTAAGCTG AATGTCAAAA CTAAAGAAAC TTGGGTTTGG 
CAAGAGCCTG ATTCATACCC ATCAGAACCC ATCTTTGTTT CTCACCCAGA TGCCTTGGAA 
GAAGATGATG GTGTAGTTCT GAGTGTGGTG GTGAGCCCAG GAGCAGGACA AAAGCCTGCT 
TATCTCCTGA TTCTGAATGC AAAGGACTTA AGTGAAGTTG CCCGGGCTGA AGTGGAGATT 
AACATCCCTG TCACCTTTCA CGGATTGTTC AAAAAATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011937677.1
CDS	536..2137
Translation	MSIQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNVYPVGEDYYACTETNFITKINPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLITLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQInullYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

Target ORF information:

RefSeq Version	XM_012082287.1
Organism	Cercocebus atys(sooty mangabey)
Definition	Cercocebus atys retinal pigment epithelium-specific protein 65kDa (RPE65), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012082287.1

ORF Insert Sequence:

ATGTCTATCC AGGTCGAGCA TCCTGCTGGT GGTTACAAGA AGCTGTTTGA AACTGTGGAG 
GAACTGTCCT CGCCGCTCAC GGCTCATGTA ACAGGCAGGA TCCCCCTGTG GCTCACCGGC 
AGTCTCCTCC GATGTGGGCC AGGACTCTTT GAAGTTGGAT CTGAGCCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT GCACAAATTT GACTTTAAAG AAGGACACGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTTC GAGGAGTGGA GGTTACCGAC AACGCCCTTG TTAATGTCTA CCCAGTGGGG 
GAAGACTACT ACGCTTGCAC AGAGACCAAC TTTATTACAA AGATTAATCC AGAGACCTTG 
GAGACAATTA AGCAGGTTGA TCTTTGCAAC TACGTCTCCG TCAATGGAGC CACTGCTCAC 
CCCCACATTG AAAATGATGG AACCGTTTAC AATATTGGTA ATTGCTTTGG AAAAAATTTT 
TCAATTGCCT ACAACATTGT AAAGATCCCA CCACTGCAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTT 
CATAGTTTTG GTTTGACTCC CAACTATATC GTTTTTGTGG AGACACCAGT CAAAATTAAC 
CTGTTCAAGT TCCTTTCTTC ATGGAGTCTT TGGGGAGCCA ACTACATGGA TTGCTTTGAG 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGGAA AAAGTACCTC 
AATAATAAAT ACAGGACTTC TCCTTTCAAC CTCTTCCATC ACATCAACAC CTATGAAGAC 
AATGGGTTTC TGATTGTGGA TCTCTGCTGC TGGAAAGGAT TTGAGTTTGT TTATAATTAC 
TTATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCCCAACCTG AAGTTAGGAG ATATGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAA TCACGCTCCC CAATACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACA 
ATCTGGCTGG AGCCTGAGGT TCTCTTTTCA GGGCCTCGCC AAGCGTTTGA GTTTCCTCAA 
ATCNNATACC AGAAGTATTG TGGGAAACCT TACACATACG CGTATGGACT TGGCTTGAAT 
CACTTTGTTC CAGATAGGCT CTGTAAGCTG AATGTCAAAA CTAAAGAAAC TTGGGTTTGG 
CAAGAGCCTG ATTCATACCC ATCAGAACCC ATCTTTGTTT CTCACCCAGA TGCCTTGGAA 
GAAGATGATG GTGTAGTTCT GAGTGTGGTG GTGAGCCCAG GAGCAGGACA AAAGCCTGCT 
TATCTCCTGA TTCTGAATGC AAAGGACTTA AGTGAAGTTG CCCGGGCTGA AGTGGAGATT 
AACATCCCTG TCACCTTTCA CGGATTGTTC AAAAAATCTT GA