CH25H cDNA ORF clone, Cercocebus atys(sooty mangabey)

Gene
Clones
gRNAs

Summary

Gene Symbol	CH25H
Entrez Gene ID	105587154
Full Name	cholesterol 25-hydroxylase
Gene Type	protein-coding
Organism	Cercocebus atys(sooty mangabey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012063134.1	XP_011918524.1	cholesterol 25-hydroxylase

Pathways from BioSystems
Gene Ontology

The following CH25H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CH25H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe208182	XM_012063134.1 Latest version!	Cercocebus atys cholesterol 25-hydroxylase (CH25H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CH25H ( XM_012063134.1 ) cDNA ORF clone, Cercocebus atys(sooty mangabey) -> XP_011918524.1 Cercocebus atys cholesterol 25-hydroxylase (CH25H), mRNA.

Price & Availability↑

CloneID	OCe208182
Clone ID Related Accession (Same CDS sequence)	XM_012063134.1
Accession Version	XM_012063134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-29
Organism	Cercocebus atys(sooty mangabey)
Product	cholesterol 25-hydroxylase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012000472.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cercocebus atys Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCC AGAACTCCTC CGACCCCCAG GTCCTTTGCA GCTCCGAGCA GCTGTTCCTG 
CAGCCCCTGT GGGACCACCT GAGGAGCTGG GAGGCCCTCA TACAGTCGCC CTTCTTCCCG 
GTCATCTTCT CCATCACCAC ATACGTGGCC TTTTGCCTGC CCTTCGTGGT CCTGGACATC 
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCCGACTT CTCGCCGTCC 
GCGAGGCAGC TCTTACCCTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC 
CCCGTGACGC TGCTGCATTG GGCCAGCAGG CCAGCCCTCC TGCCCCACGA AGCCCCCGAG 
CTGCTCCTGC TGCTGCACCA CATCGTGTTC TGCCTGCTAC TCTTCGACAC GGAGTTCTTC 
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC 
CACCAGAACC CGTCCTCGTT CGCGCTGGCC ACGCAGTATA TGAGCGTCTG GGAACTGTTT 
TCTTTGGGCT GCTTCGACAT GATGAACGTC ACGCTGCTCG GGTGCCATCC GCTCACCTCC 
CTGACCTTCC ACGTGGTCAA CATCTGGCTG TCCGTGGAGG ACCACTCCGG CTACAACTTC 
CCTTGGTCCA CTCACAGACT GGTGCCGTTC GGGTGGTACG GAGGTGTGGT GCACCACGAC 
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA 
TTGGGAACGC TGCGGACTGC ATCTGTCACA GCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011918524.1
CDS	21..839
Translation	MSSQNSSDPQVLCSSEQLFLQPLWDHLRSWEALIQSPFFPVIFSITTYVAFCLPFVVLDILCSWVPALRRYKIHPDFSPSARQLLPCLGQTLYQHVMFVFPVTLLHWASRPALLPHEAPELLLLLHHIVFCLLLFDTEFFVWHLLHHKVPWLYRTFHKVHHQNPSSFALATQYMSVWELFSLGCFDMMNVTLLGCHPLTSLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVTAR

Target ORF information:

RefSeq Version	XM_012063134.1
Organism	Cercocebus atys(sooty mangabey)
Definition	Cercocebus atys cholesterol 25-hydroxylase (CH25H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012063134.1

ORF Insert Sequence:

ATGAGCTCCC AGAACTCCTC CGACCCCCAG GTCCTTTGCA GCTCCGAGCA GCTGTTCCTG 
CAGCCCCTGT GGGACCACCT GAGGAGCTGG GAGGCCCTCA TACAGTCGCC CTTCTTCCCG 
GTCATCTTCT CCATCACCAC ATACGTGGCC TTTTGCCTGC CCTTCGTGGT CCTGGACATC 
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCCGACTT CTCGCCGTCC 
GCGAGGCAGC TCTTACCCTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC 
CCCGTGACGC TGCTGCATTG GGCCAGCAGG CCAGCCCTCC TGCCCCACGA AGCCCCCGAG 
CTGCTCCTGC TGCTGCACCA CATCGTGTTC TGCCTGCTAC TCTTCGACAC GGAGTTCTTC 
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC 
CACCAGAACC CGTCCTCGTT CGCGCTGGCC ACGCAGTATA TGAGCGTCTG GGAACTGTTT 
TCTTTGGGCT GCTTCGACAT GATGAACGTC ACGCTGCTCG GGTGCCATCC GCTCACCTCC 
CTGACCTTCC ACGTGGTCAA CATCTGGCTG TCCGTGGAGG ACCACTCCGG CTACAACTTC 
CCTTGGTCCA CTCACAGACT GGTGCCGTTC GGGTGGTACG GAGGTGTGGT GCACCACGAC 
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA 
TTGGGAACGC TGCGGACTGC ATCTGTCACA GCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.