PIWIL1 cDNA ORF clone, Cariama cristata(Red-legged seriema)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIWIL1
Entrez Gene ID	104160860
Full Name	piwi like RNA-mediated gene silencing 1
Gene Type	protein-coding
Organism	Cariama cristata(Red-legged seriema)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009699102.1	XP_009697404.1	piwi-like protein 1

Pathways from BioSystems
Gene Ontology

The following PIWIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIWIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa457739	XM_009699102.1 Latest version!	Cariama cristata piwi-like RNA-mediated gene silencing 1 (PIWIL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIWIL1 ( XM_009699102.1 ) cDNA ORF clone, Cariama cristata(Red-legged seriema) -> XP_009697404.1 Cariama cristata piwi-like RNA-mediated gene silencing 1 (PIWIL1), mRNA.

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CloneID	OCa457739
Clone ID Related Accession (Same CDS sequence)	XM_009699102.1
Accession Version	XM_009699102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Cariama cristata(Red-legged seriema)
Product	piwi-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009613626.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cariama cristata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGAA GAGCTAGAGC CAGAGCAAGA GGAAGACCTC CAGGACAGGA GACTGCTGTT 
CCTTCCGTAG GAGCTGCGTC TGTTCAACAG ACTTTGCCAA GTCAGGCCCC CGGCCGTTGG 
CAACCTCAGC AGCCACGTGT TCCTCCATCA GTATCAGAAG AACCTATCGG TCCACATCGT 
GGACGACAGA GGGGCCCTCA GAGTGTTCCC AAAACAATAG GATTACAGAT TTCTGCAGGA 
TTTCAGGAAT TGTCTTTAAC AGATAGAGGT AGATGTCGTA AAGATTTCCA TGACCTCGGG 
GTAAACGCTC GGCAAGCCAT AGAACATGTT CAAGAATCAA AAACTGGTTC TTCAGGTACT 
ATGATAAAAT TAACTGCAAA TTACTTCCGT TTGATGTCTC GACCCCAGTG GGCTTTATAT 
CAGTACCATG TTGGCTATAG TCCTGAGATG GAAGCACGCC GTCTTCGATC GGCTTTGCTC 
TTTCAGCATG AAGAGCTAAT TGGAAAGACA CATGCATTTG ATGGATCAAT ATTATTCCTG 
CCAAAAAACC TAGGGAATAA GATTACTGAA GTGTTTAGTA GGACTCGAAA TGGAGAGGAT 
GTGAAAATAA CAATCACGCT GACTAATGAG TTGCCACCTA CTTCACCTAC ATGTCTGCAG 
TTTTACAACA TCATTTTCAG AAGGCTTTTG AAGATGTTGA ATTTGCAGCA GATTGGACGT 
AATTATTACA ACCCCAGTGA CCCAATCAGC ATCCCTACTC ACAGGTTGAT GGTTTGGCCA 
GGCTTCACAA GTTCTATCCT CCAGTATGAG GAAAGCATTA TGTTATGTAC AGATGTGAGC 
CATAAGGTTC TTCGCAGTGA AACAGTGTTG GATTTTATGT ACAATCTGTA TTACCAGGTT 
GAAGAGCAAA GATTTAGAGA TGCCTGTGCT AAAGAGCTGA TAGGTTTAAT TGTTCTTACA 
AAGTATAATA ACAGAACATA CAGAGTTGAT GACATTGACT GGGATGGCAA TCCACAGTGT 
ACCTTTAGAA AAGCAGATGG TTCTGAGATC AGCTTCGTGG ACTACTACAA AAAGCAATAT 
AATCAAGAAA TTACTGACTT GAACCAGCCT GTCTTGATCA GTCAGTCTAG GAGGAAGAGA 
GGAAGCATGA TGCCAGGACC TGTGGTTCTA ATTCCAGAAC TGTGCTTCCT GACAGGATTA 
ACTGAGAAGA TGCGTAATGA TTTTAATATG ATGAAAGACC TGGCTACTCA TACACGACTG 
CCACCTGAGA AAAGGCAACG TGAAATTGGA AGACTTATTG ACTACATCCG AAAAGATGAC 
AATGTTCAGA AGGAACTCAG AGACTGGGGT TTAAGCTTTG ATTCTAATTT ATTATCCTTT 
ACGGGAAGAG TTGTTCAAAG AGAGAAGATC CTTCAATCAG GAAAAGTGTT TGATTACAAC 
CCTCAGTTTG CTGATTGGTC AAAGGAAACT AGGGGAACTC CCTTAATCTG TGCAAAGCCT 
CTGGACAACT GGTTATTAAT ATACACACGG CGCAACTATG ATGCTGCTAA TACATTACTT 
CAGAATCTGT TTAAAGTCAC GCCATCTATG GGAATCAGAA TGAATAAGGC AACCATGATT 
GAAGTAGAGG ATAGAACAGA AGCTTATTTA AGGGTTTTGC AGCAAAGTAT TACCCCTGAC 
ACAAACATAG CAGTTTGTGT TTTGTCTAGT TCTCGAAAGG ATAAGTATGA TGCTATCAAG 
AAATACTTAT GTACAGACTG TCCCATTCCA AGTCAGTGTG TGATTGCTCG CACTTTAAGC 
AAGCCTCAGA CTGCTATGGC CATAACAACA AAAATTGCCT TACAAATGAA CTGTAAAATG 
GGTGGAGAAC TTTGGAGTGT TGAGATCCCA CTGAAACAGG TAATGGTTGT GGGAATTGAT 
TGTTACCATG ACACTTTATC TGGAAAGCAG TCAGTTGCGG GATTTGTCGC TAGCCTGAAT 
CAAACAATGA CACGGTGGTT CTCTCGCTGT GTTGTTCAGG GTCGTGGGCA AGAACTTGTG 
GATGGGCTCA AAGCCTGCTT GCAAACTGCC CTAAGAGACT GGTTCAAATG GAATAAGTAT 
TTGCCCTCTC GTATTGTTGT GTATCGTGAT GGTGTAGGAG ATGGACAACT AAACACTTTG 
GTTAATTACG AAGTGCCCCA ATTTCTGGAT TGCTTGAAGA GTGTTGGTGA AGACTACAAT 
CCGAGACTTA CTGTGATAGT TGTGAAGAAA CGAGTGAATA CCAGGTTCTT TGCAGAGTGT 
GATGGAGGAT TTCAGAACCC ACCCCCTGGG ACTGTTGTTG ATGTGGAGGT TACCAGACCA 
GAATGGTATG ATTTCTTTAT TGTGAGTCAG GCAGTGAGAA ATGGTTGTGT TGCACCCACT 
CATTACAACG TAATTTATGA CACTAGCAAA CTGAAACCAG ATCACATGCA ACGTTTAACC 
TACAAACTTT GCCACATGTA CTATAACTGG TCGGGTGTTA TCAGAGTACC TGCTCCTTGC 
CAATATGCTC ATAAACTGGC TTTCCTTGTT GGTCAGAGTA TTCACAGAGA ACCAAACCTG 
TTGCTTTCAG ACAGACTCTA CTATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009697404.1
CDS	1..2610
Translation	MTGRARARARGRPPGQETAVPSVGAASVQQTLPSQAPGRWQPQQPRVPPSVSEEPIGPHRGRQRGPQSVPKTIGLQISAGFQELSLTDRGRCRKDFHDLGVNARQAIEHVQESKTGSSGTMIKLTANYFRLMSRPQWALYQYHVGYSPEMEARRLRSALLFQHEELIGKTHAFDGSILFLPKNLGNKITEVFSRTRNGEDVKITITLTNELPPTSPTCLQFYNIIFRRLLKMLNLQQIGRNYYNPSDPISIPTHRLMVWPGFTSSILQYEESIMLCTDVSHKVLRSETVLDFMYNLYYQVEEQRFRDACAKELIGLIVLTKYNNRTYRVDDIDWDGNPQCTFRKADGSEISFVDYYKKQYNQEITDLNQPVLISQSRRKRGSMMPGPVVLIPELCFLTGLTEKMRNDFNMMKDLATHTRLPPEKRQREIGRLIDYIRKDDNVQKELRDWGLSFDSNLLSFTGRVVQREKILQSGKVFDYNPQFADWSKETRGTPLICAKPLDNWLLIYTRRNYDAANTLLQNLFKVTPSMGIRMNKATMIEVEDRTEAYLRVLQQSITPDTNIAVCVLSSSRKDKYDAIKKYLCTDCPIPSQCVIARTLSKPQTAMAITTKIALQMNCKMGGELWSVEIPLKQVMVVGIDCYHDTLSGKQSVAGFVASLNQTMTRWFSRCVVQGRGQELVDGLKACLQTALRDWFKWNKYLPSRIVVYRDGVGDGQLNTLVNYEVPQFLDCLKSVGEDYNPRLTVIVVKKRVNTRFFAECDGGFQNPPPGTVVDVEVTRPEWYDFFIVSQAVRNGCVAPTHYNVIYDTSKLKPDHMQRLTYKLCHMYYNWSGVIRVPAPCQYAHKLAFLVGQSIHREPNLLLSDRLYYL

Target ORF information:

RefSeq Version	XM_009699102.1
Organism	Cariama cristata(Red-legged seriema)
Definition	Cariama cristata piwi-like RNA-mediated gene silencing 1 (PIWIL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009699102.1

ORF Insert Sequence:

ATGACAGGAA GAGCTAGAGC CAGAGCAAGA GGAAGACCTC CAGGACAGGA GACTGCTGTT 
CCTTCCGTAG GAGCTGCGTC TGTTCAACAG ACTTTGCCAA GTCAGGCCCC CGGCCGTTGG 
CAACCTCAGC AGCCACGTGT TCCTCCATCA GTATCAGAAG AACCTATCGG TCCACATCGT 
GGACGACAGA GGGGCCCTCA GAGTGTTCCC AAAACAATAG GATTACAGAT TTCTGCAGGA 
TTTCAGGAAT TGTCTTTAAC AGATAGAGGT AGATGTCGTA AAGATTTCCA TGACCTCGGG 
GTAAACGCTC GGCAAGCCAT AGAACATGTT CAAGAATCAA AAACTGGTTC TTCAGGTACT 
ATGATAAAAT TAACTGCAAA TTACTTCCGT TTGATGTCTC GACCCCAGTG GGCTTTATAT 
CAGTACCATG TTGGCTATAG TCCTGAGATG GAAGCACGCC GTCTTCGATC GGCTTTGCTC 
TTTCAGCATG AAGAGCTAAT TGGAAAGACA CATGCATTTG ATGGATCAAT ATTATTCCTG 
CCAAAAAACC TAGGGAATAA GATTACTGAA GTGTTTAGTA GGACTCGAAA TGGAGAGGAT 
GTGAAAATAA CAATCACGCT GACTAATGAG TTGCCACCTA CTTCACCTAC ATGTCTGCAG 
TTTTACAACA TCATTTTCAG AAGGCTTTTG AAGATGTTGA ATTTGCAGCA GATTGGACGT 
AATTATTACA ACCCCAGTGA CCCAATCAGC ATCCCTACTC ACAGGTTGAT GGTTTGGCCA 
GGCTTCACAA GTTCTATCCT CCAGTATGAG GAAAGCATTA TGTTATGTAC AGATGTGAGC 
CATAAGGTTC TTCGCAGTGA AACAGTGTTG GATTTTATGT ACAATCTGTA TTACCAGGTT 
GAAGAGCAAA GATTTAGAGA TGCCTGTGCT AAAGAGCTGA TAGGTTTAAT TGTTCTTACA 
AAGTATAATA ACAGAACATA CAGAGTTGAT GACATTGACT GGGATGGCAA TCCACAGTGT 
ACCTTTAGAA AAGCAGATGG TTCTGAGATC AGCTTCGTGG ACTACTACAA AAAGCAATAT 
AATCAAGAAA TTACTGACTT GAACCAGCCT GTCTTGATCA GTCAGTCTAG GAGGAAGAGA 
GGAAGCATGA TGCCAGGACC TGTGGTTCTA ATTCCAGAAC TGTGCTTCCT GACAGGATTA 
ACTGAGAAGA TGCGTAATGA TTTTAATATG ATGAAAGACC TGGCTACTCA TACACGACTG 
CCACCTGAGA AAAGGCAACG TGAAATTGGA AGACTTATTG ACTACATCCG AAAAGATGAC 
AATGTTCAGA AGGAACTCAG AGACTGGGGT TTAAGCTTTG ATTCTAATTT ATTATCCTTT 
ACGGGAAGAG TTGTTCAAAG AGAGAAGATC CTTCAATCAG GAAAAGTGTT TGATTACAAC 
CCTCAGTTTG CTGATTGGTC AAAGGAAACT AGGGGAACTC CCTTAATCTG TGCAAAGCCT 
CTGGACAACT GGTTATTAAT ATACACACGG CGCAACTATG ATGCTGCTAA TACATTACTT 
CAGAATCTGT TTAAAGTCAC GCCATCTATG GGAATCAGAA TGAATAAGGC AACCATGATT 
GAAGTAGAGG ATAGAACAGA AGCTTATTTA AGGGTTTTGC AGCAAAGTAT TACCCCTGAC 
ACAAACATAG CAGTTTGTGT TTTGTCTAGT TCTCGAAAGG ATAAGTATGA TGCTATCAAG 
AAATACTTAT GTACAGACTG TCCCATTCCA AGTCAGTGTG TGATTGCTCG CACTTTAAGC 
AAGCCTCAGA CTGCTATGGC CATAACAACA AAAATTGCCT TACAAATGAA CTGTAAAATG 
GGTGGAGAAC TTTGGAGTGT TGAGATCCCA CTGAAACAGG TAATGGTTGT GGGAATTGAT 
TGTTACCATG ACACTTTATC TGGAAAGCAG TCAGTTGCGG GATTTGTCGC TAGCCTGAAT 
CAAACAATGA CACGGTGGTT CTCTCGCTGT GTTGTTCAGG GTCGTGGGCA AGAACTTGTG 
GATGGGCTCA AAGCCTGCTT GCAAACTGCC CTAAGAGACT GGTTCAAATG GAATAAGTAT 
TTGCCCTCTC GTATTGTTGT GTATCGTGAT GGTGTAGGAG ATGGACAACT AAACACTTTG 
GTTAATTACG AAGTGCCCCA ATTTCTGGAT TGCTTGAAGA GTGTTGGTGA AGACTACAAT 
CCGAGACTTA CTGTGATAGT TGTGAAGAAA CGAGTGAATA CCAGGTTCTT TGCAGAGTGT 
GATGGAGGAT TTCAGAACCC ACCCCCTGGG ACTGTTGTTG ATGTGGAGGT TACCAGACCA 
GAATGGTATG ATTTCTTTAT TGTGAGTCAG GCAGTGAGAA ATGGTTGTGT TGCACCCACT 
CATTACAACG TAATTTATGA CACTAGCAAA CTGAAACCAG ATCACATGCA ACGTTTAACC 
TACAAACTTT GCCACATGTA CTATAACTGG TCGGGTGTTA TCAGAGTACC TGCTCCTTGC 
CAATATGCTC ATAAACTGGC TTTCCTTGTT GGTCAGAGTA TTCACAGAGA ACCAAACCTG 
TTGCTTTCAG ACAGACTCTA CTATCTTTGA