GRAMD1A cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRAMD1A
Entrez Gene ID	484585
Full Name	GRAM domain containing 1A
General protein information	Preferred Names GRAM domain containing 1A Names GRAM domain-containing protein 1A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005616728.2	XP_005616785.1	GRAM domain-containing protein 1A isoform X2
XM_005616727.2	XP_005616784.1	GRAM domain-containing protein 1A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GRAMD1A	XP_005616784.1
Bos taurus (cattle)	GRAMD1A	XP_002694974.1
Rattus norvegicus (Norway rat)	Gramd1a	NP_001014182.1
Pan troglodytes (chimpanzee)	GRAMD1A	XP_001157243.2
Mus musculus (house mouse)	Gramd1a	NP_082174.3
Xenopus tropicalis (tropical clawed frog)	gramd1a	XP_002940577.2
Danio rerio (zebrafish)	gramd1a	XP_683776.6
Homo sapiens (human)	GRAMD1A	NP_065946.2

Gene Ontology

The following GRAMD1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRAMD1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe02376	XM_005616727.2 Latest version!	Canis lupus familiaris GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe02377	XM_005616728.2 Latest version!	Canis lupus familiaris GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRAMD1A ( XM_005616727.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005616784.1 Canis lupus familiaris GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe02376
Clone ID Related Accession (Same CDS sequence)	XM_005616727.2
Accession Version	XM_005616727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	GRAM domain-containing protein 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TCCTGCCCCA GCCACCTTGG CCCCCATCAG CCGAGGGGGG CGACCCGGGC 
GCATCAGGGC CCCCAGCCGA GTGGCAGAGC CAGCCCCCAC AGGGTCGCAG GGCGGACGGC 
CTGGCCCCTG AGCCCTGGAG GCTTCTGGAG GTGCCCTCCA TTCACATAAC ACCATGCAGT 
GACGGAGAGT CACCCCCTGG CACCCCAACC CCCCAGCGCC TACAGCTGCC GAGGACCCCA 
GACCCGGAGA GTCGACCCCG GGACAGCACC ACCCCCCATT CCGGCCGGAG CACTCCAAGC 
AGCTCCCCGT CTCTCCGGAA GCGGCTGCAG CTCTTGCCCT CAAGCCGGCC CCCGCCTGAG 
CCTGAACCGG GCACCATGGT GGAGAAGGGG TCAGACAGCT CCTCAGAGAA GGGTGGGGGG 
CCTGGGACAC CCAGCACCCA GAGCCTGGGC AGCCGGAACT TCATCCGCAA TAGCAAGAAG 
ATGCAGAGCT GGTACAGCAT GCTGAGCCCC ACGTACAAAC AGCGGAACGA GGACTTCCGG 
AAGCTGTTCA GCAAACTCCC AGAGGCCGAA CGCCTCATCG TGGATTACTC CTGTGCCTTG 
CAGCGCGAGA TCCTCCTCCA GGGCCGCCTC TACCTCTCTG AGAACTGGAT CTGCTTCTAC 
AGCAACATCT TCCGCTGGGA AACCACAATT TCCATCCAAC TGAAGGAAGT GACGTGCCTG 
AAGAAGGAGA AGACAGCCAA GCTGATCCCC AATGCCATCC AGATCTGCAC TGAGAGCGAG 
AAGCATTTCT TCACCTCCTT CGGGGCCCGG GACCGCTGCT TCCTCCTCAT CTTTCGCCTG 
TGGCAGAATG CGCTGCTTGA AAAGACGCTG AGTCCCCGCG AGCTCTGGCA CTTGGTGCAT 
CAGTGCTACG GCTCAGAGCT GGGCCTCACC AGTGAGGATG AGGACTATGT CTGCCCCTTG 
CAGCTGAACG GTCTGGGGAG CCCCAAGGAA GTGGGAGATG TGATTGCCCT GAGTGACATC 
ACCCCCTCGG GGGCAGCCGA CAACAGCCAG GAGCCGAGCC CTGTGGGTTC GCGCCGTGGC 
CGCCTCACCC CCAACCTTTC CCGGGCCAGC AGTGATGCAG ACCACGTGGC AGAGGAGGAC 
AAGGAGGAGC AGACAGACAG CCAGCCAGAC GCCTCCTCCA GCCAGACAGT GACCCCGGTG 
GCTGAACCCC CAAGCACAGA GCCTGCCCCG CCCAACGGGC CCGCCTCCCT GGGCCCCTTG 
GATCTCCTGC CCAGTGAGGA AGTGTTGACA GACACCAGTA ACTCCTCCTC GTCCACAGGG 
GAGGAAGCTG ACCTGGCCGC CCTGCTTCCC GACCTCTCCG GCCGCCTCCT CATCAACTCC 
GTCTTCCACG TGGGCGCAGA GCGGCTGCAG CAGATGCTCT TCTCAGACTC GCCCTTCCTG 
CAGGGCTTCC TGCAGCAGTG CAAGTTCACA GATGTGACCT TGAGTCCCTG GAGTGGGGAC 
AACAAGTGCC ACCAGCGCCG AGTGCTGACG TATACCATTC CCATCAGCAA CCCCCTGGGC 
CCCAAGAGCG CGTCGGTGGT GGAGACACAG ACACTGTTCC GGCGTGGCCC CCAGGCAGGC 
GGCTGTGTGG TGGACTCGGA GGTGCTGACA CAGGGCATCC CGTACCAGGA CTACTTCTAC 
ACTGCCCACC GCTACTGCAT CCTGGGCCTT GCCCGGAACA AGGCCCGGCT CCGAGTGTCC 
TCCGAGATCC GCTACCGGAA GCAGCCCTGG AGCCTCGTGA AGTCACTCAT TGAGAAGAAC 
TCTTGGAGCG GCATTGAGGA TTACTTCCAC CACCTGGAGC GAGAGCTTGC CAAAGCCGAG 
AAGCTGTCCC TGGAGGAAGG CGGGAAGGAT GCCCGAGGAT TGCTGTCAGG CCTGAGGAGG 
CGCAAGCGGC CCCTGAGCTG GAGGGGTCAC GGCGACGGGC CCCAGCACCC GGACCCCGAC 
CCCTGTGCCC GGGCTGGCAT GCACACCTCG GGTTCCCTCA GCTCGCGCTT CTCGGAACCA 
TCCGTGGACC AGGGCCCCGG GGCGGGCATC CCCAGCGCCC TGGTTCTCAT CAGCATTGTG 
ATCTGTGTGA GCCTCATCGT CCTCATCGCC CTCAATGTCT TGCTCTTTTA CCGCCTCTGG 
TCCCTGGAGA GGACGGCCCA CACCTTCGAG TCCTGGCACA GTCTGGCCCT GGCCAAGGGC 
AAGTTCCCCC AGACGGCCAC GGAGTGGGCG GAGATCCTAG CCCTGCAGAA GCAGTTCCAC 
AGCGTCGAGG TGCACAAGTG GAGGCAGATC CTGCGGGCTT CCGTGGAGCT TCTAGACGAG 
ATGAAGTTCT CGCTGGAGAA GCTGCATCAA GGCATCACTG TCTCAGACCC CCCCTTCGAC 
TCCCAGCCAC GGCCCGATGA CAGCTTCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616784.1
CDS	16..2448
Translation	MAQLLPQPPWPPSAEGGDPGASGPPAEWQSQPPQGRRADGLAPEPWRLLEVPSIHITPCSDGESPPGTPTPQRLQLPRTPDPESRPRDSTTPHSGRSTPSSSPSLRKRLQLLPSSRPPPEPEPGTMVEKGSDSSSEKGGGPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKEVGDVIALSDITPSGAADNSQEPSPVGSRRGRLTPNLSRASSDADHVAEEDKEEQTDSQPDASSSQTVTPVAEPPSTEPAPPNGPASLGPLDLLPSEEVLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQGFLQQCKFTDVTLSPWSGDNKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDARGLLSGLRRRKRPLSWRGHGDGPQHPDPDPCARAGMHTSGSLSSRFSEPSVDQGPGAGIPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPRPDDSFS

Target ORF information:

RefSeq Version	XM_005616727.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005616727.2

ORF Insert Sequence:

ATGGCCCAGC TCCTGCCCCA GCCACCTTGG CCCCCATCAG CCGAGGGGGG CGACCCGGGC 
GCATCAGGGC CCCCAGCCGA GTGGCAGAGC CAGCCCCCAC AGGGTCGCAG GGCGGACGGC 
CTGGCCCCTG AGCCCTGGAG GCTTCTGGAG GTGCCCTCCA TTCACATAAC ACCATGCAGT 
GACGGAGAGT CACCCCCTGG CACCCCAACC CCCCAGCGCC TACAGCTGCC GAGGACCCCA 
GACCCGGAGA GTCGACCCCG GGACAGCACC ACCCCCCATT CCGGCCGGAG CACTCCAAGC 
AGCTCCCCGT CTCTCCGGAA GCGGCTGCAG CTCTTGCCCT CAAGCCGGCC CCCGCCTGAG 
CCTGAACCGG GCACCATGGT GGAGAAGGGG TCAGACAGCT CCTCAGAGAA GGGTGGGGGG 
CCTGGGACAC CCAGCACCCA GAGCCTGGGC AGCCGGAACT TCATCCGCAA TAGCAAGAAG 
ATGCAGAGCT GGTACAGCAT GCTGAGCCCC ACGTACAAAC AGCGGAACGA GGACTTCCGG 
AAGCTGTTCA GCAAACTCCC AGAGGCCGAA CGCCTCATCG TGGATTACTC CTGTGCCTTG 
CAGCGCGAGA TCCTCCTCCA GGGCCGCCTC TACCTCTCTG AGAACTGGAT CTGCTTCTAC 
AGCAACATCT TCCGCTGGGA AACCACAATT TCCATCCAAC TGAAGGAAGT GACGTGCCTG 
AAGAAGGAGA AGACAGCCAA GCTGATCCCC AATGCCATCC AGATCTGCAC TGAGAGCGAG 
AAGCATTTCT TCACCTCCTT CGGGGCCCGG GACCGCTGCT TCCTCCTCAT CTTTCGCCTG 
TGGCAGAATG CGCTGCTTGA AAAGACGCTG AGTCCCCGCG AGCTCTGGCA CTTGGTGCAT 
CAGTGCTACG GCTCAGAGCT GGGCCTCACC AGTGAGGATG AGGACTATGT CTGCCCCTTG 
CAGCTGAACG GTCTGGGGAG CCCCAAGGAA GTGGGAGATG TGATTGCCCT GAGTGACATC 
ACCCCCTCGG GGGCAGCCGA CAACAGCCAG GAGCCGAGCC CTGTGGGTTC GCGCCGTGGC 
CGCCTCACCC CCAACCTTTC CCGGGCCAGC AGTGATGCAG ACCACGTGGC AGAGGAGGAC 
AAGGAGGAGC AGACAGACAG CCAGCCAGAC GCCTCCTCCA GCCAGACAGT GACCCCGGTG 
GCTGAACCCC CAAGCACAGA GCCTGCCCCG CCCAACGGGC CCGCCTCCCT GGGCCCCTTG 
GATCTCCTGC CCAGTGAGGA AGTGTTGACA GACACCAGTA ACTCCTCCTC GTCCACAGGG 
GAGGAAGCTG ACCTGGCCGC CCTGCTTCCC GACCTCTCCG GCCGCCTCCT CATCAACTCC 
GTCTTCCACG TGGGCGCAGA GCGGCTGCAG CAGATGCTCT TCTCAGACTC GCCCTTCCTG 
CAGGGCTTCC TGCAGCAGTG CAAGTTCACA GATGTGACCT TGAGTCCCTG GAGTGGGGAC 
AACAAGTGCC ACCAGCGCCG AGTGCTGACG TATACCATTC CCATCAGCAA CCCCCTGGGC 
CCCAAGAGCG CGTCGGTGGT GGAGACACAG ACACTGTTCC GGCGTGGCCC CCAGGCAGGC 
GGCTGTGTGG TGGACTCGGA GGTGCTGACA CAGGGCATCC CGTACCAGGA CTACTTCTAC 
ACTGCCCACC GCTACTGCAT CCTGGGCCTT GCCCGGAACA AGGCCCGGCT CCGAGTGTCC 
TCCGAGATCC GCTACCGGAA GCAGCCCTGG AGCCTCGTGA AGTCACTCAT TGAGAAGAAC 
TCTTGGAGCG GCATTGAGGA TTACTTCCAC CACCTGGAGC GAGAGCTTGC CAAAGCCGAG 
AAGCTGTCCC TGGAGGAAGG CGGGAAGGAT GCCCGAGGAT TGCTGTCAGG CCTGAGGAGG 
CGCAAGCGGC CCCTGAGCTG GAGGGGTCAC GGCGACGGGC CCCAGCACCC GGACCCCGAC 
CCCTGTGCCC GGGCTGGCAT GCACACCTCG GGTTCCCTCA GCTCGCGCTT CTCGGAACCA 
TCCGTGGACC AGGGCCCCGG GGCGGGCATC CCCAGCGCCC TGGTTCTCAT CAGCATTGTG 
ATCTGTGTGA GCCTCATCGT CCTCATCGCC CTCAATGTCT TGCTCTTTTA CCGCCTCTGG 
TCCCTGGAGA GGACGGCCCA CACCTTCGAG TCCTGGCACA GTCTGGCCCT GGCCAAGGGC 
AAGTTCCCCC AGACGGCCAC GGAGTGGGCG GAGATCCTAG CCCTGCAGAA GCAGTTCCAC 
AGCGTCGAGG TGCACAAGTG GAGGCAGATC CTGCGGGCTT CCGTGGAGCT TCTAGACGAG 
ATGAAGTTCT CGCTGGAGAA GCTGCATCAA GGCATCACTG TCTCAGACCC CCCCTTCGAC 
TCCCAGCCAC GGCCCGATGA CAGCTTCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_005616728.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005616785.1 Canis lupus familiaris GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe02377
Clone ID Related Accession (Same CDS sequence)	XM_005616728.2
Accession Version	XM_005616728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	GRAM domain-containing protein 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC TCCTGCCCCA GCCACCTTGG CCCCCATCAG CCGAGGGGGG CGACCCGGGC 
GCATCAGGGC CCCCAGCCGA GTGGCAGAGC CAGCCCCCAC AGGGTCGCAG GGCGGACGGC 
CTGGCCCCTG AGCCCTGGAG GCTTCTGGAG GTGCCCTCCA TTCACATAAC ACCATGCAGT 
GACGGAGAGT CACCCCCTGG CACCCCAACC CCCCAGCGCC TACAGCTGCC GAGGACCCCA 
GACCCGGAGA GTCGACCCCG GGACAGCACC ACCCCCCATT CCGGCCGGAG CACTCCAAGC 
AGCTCCCCGT CTCTCCGGAA GCGGCTGCAG CTCTTGCCCT CAAGCCGGCC CCCGCCTGAG 
CCTGAACCGG GCACCATGGT GGAGAAGGGG TCAGACAGCT CCTCAGAGAA GGGTGGGGGG 
CCTGGGACAC CCAGCACCCA GAGCCTGGGC AGCCGGAACT TCATCCGCAA TAGCAAGAAG 
ATGCAGAGCT GGTACAGCAT GCTGAGCCCC ACGTACAAAC AGCGGAACGA GGACTTCCGG 
AAGCTGTTCA GCAAACTCCC AGAGGCCGAA CGCCTCATCG TGGATTACTC CTGTGCCTTG 
CAGCGCGAGA TCCTCCTCCA GGGCCGCCTC TACCTCTCTG AGAACTGGAT CTGCTTCTAC 
AGCAACATCT TCCGCTGGGA AACCACAATT TCCATCCAAC TGAAGGAAGT GACGTGCCTG 
AAGAAGGAGA AGACAGCCAA GCTGATCCCC AATGCCATCC AGATCTGCAC TGAGAGCGAG 
AAGCATTTCT TCACCTCCTT CGGGGCCCGG GACCGCTGCT TCCTCCTCAT CTTTCGCCTG 
TGGCAGAATG CGCTGCTTGA AAAGACGCTG AGTCCCCGCG AGCTCTGGCA CTTGGTGCAT 
CAGTGCTACG GCTCAGAGCT GGGCCTCACC AGTGAGGATG AGGACTATGT CTGCCCCTTG 
CAGCTGAACG GTCTGGGGAG CCCCAAGGAA GTGGGAGATG TGATTGCCCT GAGTGACATC 
ACCCCCTCGG GGGCAGCCGA CAACAGCCAG GAGCCGAGCC CTGTGGGTTC GCGCCGTGGC 
CGCCTCACCC CCAACCTTTC CCGGGCCAGC AGTGATGCAG ACCACGTGGC AGAGGAGGAC 
AAGGAGGAGC AGACAGACAG CCAGCCAGAC GCCTCCTCCA GCCAGACAGT GACCCCGGTG 
GCTGAACCCC CAAGCACAGA GCCTGCCCCG CCCAACGGGC CCGCCTCCCT GGGCCCCTTG 
GATCTCCTGC CCAGTGAGGA AGTGTTGACA GACACCAGTA ACTCCTCCTC GTCCACAGGG 
GAGGAAGCTG ACCTGGCCGC CCTGCTTCCC GACCTCTCCG GCCGCCTCCT CATCAACTCC 
GTCTTCCACG TGGGCGCAGA GCGGCTGCAG CAGATGCTCT TCTCAGACTC GCCCTTCCTG 
CAGGGCTTCC TGCAGCAGTG CAAGTTCACA GATGTGACCT TGAGTCCCTG GAGTGGGGAC 
AACAAGTGCC ACCAGCGCCG AGTGCTGACG TATACCATTC CCATCAGCAA CCCCCTGGGC 
CCCAAGAGCG CGTCGGTGGT GGAGACACAG ACACTGTTCC GGCGTGGCCC CCAGGCAGGC 
GGCTGTGTGG TGGACTCGGA GGTGCTGACA CAGGGCATCC CGTACCAGGA CTACTTCTAC 
ACTGCCCACC GCTACTGCAT CCTGGGCCTT GCCCGGAACA AGGCCCGGCT CCGAGTGTCC 
TCCGAGATCC GCTACCGGAA GCAGCCCTGG AGCCTCGTGA AGTCACTCAT TGAGAAGAAC 
TCTTGGAGCG GCATTGAGGA TTACTTCCAC CACCTGGAGC GAGAGCTTGC CAAAGCCGAG 
AAGCTGTCCC TGGAGGAAGG CGGGAAGGAT GCCCGAGGAT TGCTGTCAGG CCTGAGGAGG 
CGCAAGCGGC CCCTGAGCTG GAGGGGTCAC GGCGACGGGC CCCAGCACCC GGACCCCGAC 
CCCTGTGCCC GGGCTGGCAT GCACACCTCG GGTTCCCTCA GCTCGCGCTT CTCGGAACCA 
TCCGTGGACC AGGGCCCCGG GGCGGGCATC CCCAGCGCCC TGGTTCTCAT CAGCATTGTC 
CTCATCGTCC TCATCGCCCT CAATGTCTTG CTCTTTTACC GCCTCTGGTC CCTGGAGAGG 
ACGGCCCACA CCTTCGAGTC CTGGCACAGT CTGGCCCTGG CCAAGGGCAA GTTCCCCCAG 
ACGGCCACGG AGTGGGCGGA GATCCTAGCC CTGCAGAAGC AGTTCCACAG CGTCGAGGTG 
CACAAGTGGA GGCAGATCCT GCGGGCTTCC GTGGAGCTTC TAGACGAGAT GAAGTTCTCG 
CTGGAGAAGC TGCATCAAGG CATCACTGTC TCAGACCCCC CCTTCGACTC CCAGCCACGG 
CCCGATGACA GCTTCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616785.1
CDS	16..2436
Translation	MAQLLPQPPWPPSAEGGDPGASGPPAEWQSQPPQGRRADGLAPEPWRLLEVPSIHITPCSDGESPPGTPTPQRLQLPRTPDPESRPRDSTTPHSGRSTPSSSPSLRKRLQLLPSSRPPPEPEPGTMVEKGSDSSSEKGGGPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKEVGDVIALSDITPSGAADNSQEPSPVGSRRGRLTPNLSRASSDADHVAEEDKEEQTDSQPDASSSQTVTPVAEPPSTEPAPPNGPASLGPLDLLPSEEVLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQGFLQQCKFTDVTLSPWSGDNKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDARGLLSGLRRRKRPLSWRGHGDGPQHPDPDPCARAGMHTSGSLSSRFSEPSVDQGPGAGIPSALVLISIVLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPRPDDSFS

Target ORF information:

RefSeq Version	XM_005616728.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005616728.2

ORF Insert Sequence:

ATGGCCCAGC TCCTGCCCCA GCCACCTTGG CCCCCATCAG CCGAGGGGGG CGACCCGGGC 
GCATCAGGGC CCCCAGCCGA GTGGCAGAGC CAGCCCCCAC AGGGTCGCAG GGCGGACGGC 
CTGGCCCCTG AGCCCTGGAG GCTTCTGGAG GTGCCCTCCA TTCACATAAC ACCATGCAGT 
GACGGAGAGT CACCCCCTGG CACCCCAACC CCCCAGCGCC TACAGCTGCC GAGGACCCCA 
GACCCGGAGA GTCGACCCCG GGACAGCACC ACCCCCCATT CCGGCCGGAG CACTCCAAGC 
AGCTCCCCGT CTCTCCGGAA GCGGCTGCAG CTCTTGCCCT CAAGCCGGCC CCCGCCTGAG 
CCTGAACCGG GCACCATGGT GGAGAAGGGG TCAGACAGCT CCTCAGAGAA GGGTGGGGGG 
CCTGGGACAC CCAGCACCCA GAGCCTGGGC AGCCGGAACT TCATCCGCAA TAGCAAGAAG 
ATGCAGAGCT GGTACAGCAT GCTGAGCCCC ACGTACAAAC AGCGGAACGA GGACTTCCGG 
AAGCTGTTCA GCAAACTCCC AGAGGCCGAA CGCCTCATCG TGGATTACTC CTGTGCCTTG 
CAGCGCGAGA TCCTCCTCCA GGGCCGCCTC TACCTCTCTG AGAACTGGAT CTGCTTCTAC 
AGCAACATCT TCCGCTGGGA AACCACAATT TCCATCCAAC TGAAGGAAGT GACGTGCCTG 
AAGAAGGAGA AGACAGCCAA GCTGATCCCC AATGCCATCC AGATCTGCAC TGAGAGCGAG 
AAGCATTTCT TCACCTCCTT CGGGGCCCGG GACCGCTGCT TCCTCCTCAT CTTTCGCCTG 
TGGCAGAATG CGCTGCTTGA AAAGACGCTG AGTCCCCGCG AGCTCTGGCA CTTGGTGCAT 
CAGTGCTACG GCTCAGAGCT GGGCCTCACC AGTGAGGATG AGGACTATGT CTGCCCCTTG 
CAGCTGAACG GTCTGGGGAG CCCCAAGGAA GTGGGAGATG TGATTGCCCT GAGTGACATC 
ACCCCCTCGG GGGCAGCCGA CAACAGCCAG GAGCCGAGCC CTGTGGGTTC GCGCCGTGGC 
CGCCTCACCC CCAACCTTTC CCGGGCCAGC AGTGATGCAG ACCACGTGGC AGAGGAGGAC 
AAGGAGGAGC AGACAGACAG CCAGCCAGAC GCCTCCTCCA GCCAGACAGT GACCCCGGTG 
GCTGAACCCC CAAGCACAGA GCCTGCCCCG CCCAACGGGC CCGCCTCCCT GGGCCCCTTG 
GATCTCCTGC CCAGTGAGGA AGTGTTGACA GACACCAGTA ACTCCTCCTC GTCCACAGGG 
GAGGAAGCTG ACCTGGCCGC CCTGCTTCCC GACCTCTCCG GCCGCCTCCT CATCAACTCC 
GTCTTCCACG TGGGCGCAGA GCGGCTGCAG CAGATGCTCT TCTCAGACTC GCCCTTCCTG 
CAGGGCTTCC TGCAGCAGTG CAAGTTCACA GATGTGACCT TGAGTCCCTG GAGTGGGGAC 
AACAAGTGCC ACCAGCGCCG AGTGCTGACG TATACCATTC CCATCAGCAA CCCCCTGGGC 
CCCAAGAGCG CGTCGGTGGT GGAGACACAG ACACTGTTCC GGCGTGGCCC CCAGGCAGGC 
GGCTGTGTGG TGGACTCGGA GGTGCTGACA CAGGGCATCC CGTACCAGGA CTACTTCTAC 
ACTGCCCACC GCTACTGCAT CCTGGGCCTT GCCCGGAACA AGGCCCGGCT CCGAGTGTCC 
TCCGAGATCC GCTACCGGAA GCAGCCCTGG AGCCTCGTGA AGTCACTCAT TGAGAAGAAC 
TCTTGGAGCG GCATTGAGGA TTACTTCCAC CACCTGGAGC GAGAGCTTGC CAAAGCCGAG 
AAGCTGTCCC TGGAGGAAGG CGGGAAGGAT GCCCGAGGAT TGCTGTCAGG CCTGAGGAGG 
CGCAAGCGGC CCCTGAGCTG GAGGGGTCAC GGCGACGGGC CCCAGCACCC GGACCCCGAC 
CCCTGTGCCC GGGCTGGCAT GCACACCTCG GGTTCCCTCA GCTCGCGCTT CTCGGAACCA 
TCCGTGGACC AGGGCCCCGG GGCGGGCATC CCCAGCGCCC TGGTTCTCAT CAGCATTGTC 
CTCATCGTCC TCATCGCCCT CAATGTCTTG CTCTTTTACC GCCTCTGGTC CCTGGAGAGG 
ACGGCCCACA CCTTCGAGTC CTGGCACAGT CTGGCCCTGG CCAAGGGCAA GTTCCCCCAG 
ACGGCCACGG AGTGGGCGGA GATCCTAGCC CTGCAGAAGC AGTTCCACAG CGTCGAGGTG 
CACAAGTGGA GGCAGATCCT GCGGGCTTCC GTGGAGCTTC TAGACGAGAT GAAGTTCTCG 
CTGGAGAAGC TGCATCAAGG CATCACTGTC TCAGACCCCC CCTTCGACTC CCAGCCACGG 
CCCGATGACA GCTTCTCCTG A