NSUN2 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSUN2
Entrez Gene ID	478625
Full Name	NOP2/Sun RNA methyltransferase family member 2
General protein information	Preferred Names NOP2/Sun RNA methyltransferase family member 2 Names tRNA (cytosine(34)-C(5))-methyltransferase NOL1/NOP2/Sun domain family, member 2 NOP2/Sun domain family, member 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005639683.1	XP_005639740.1	tRNA (cytosine(34)-C(5))-methyltransferase isoform X2
XM_005639683.2	XP_005639740.1	tRNA (cytosine(34)-C(5))-methyltransferase isoform X2
XM_535800.5	XP_535800.2	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
XM_535800.4	XP_535800.2	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NSUN2	NP_001026175.1
Oryza sativa (rice)	Os08g0484400	NP_001062081.1
Canis lupus familiaris (dog)	NSUN2	XP_535800.2
Schizosaccharomyces pombe (fission yeast)	SPAC17D4.04	NP_594695.3
Neurospora crassa	NCU05301	XP_962072.1
Arabidopsis thaliana (thale cress)	AT4G40000	NP_195710.3
Oryza sativa (rice)	Os09g0471900	NP_001063445.1
Pan troglodytes (chimpanzee)	NSUN2	XP_517620.2
Mus musculus (house mouse)	Nsun2	NP_663329.3
Drosophila melanogaster (fruit fly)	Nsun2	NP_652007.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002504	XP_563662.3
Caenorhabditis elegans (roundworm)	Y48G8AL.5	NP_490830.2
Eremothecium gossypii	AGOS_AFR179C	NP_985726.2
Homo sapiens (human)	NSUN2	NP_060225.4
Saccharomyces cerevisiae (baker's yeast)	NCL1	NP_009529.1
Kluyveromyces lactis	KLLA0F12562g	XP_455648.1
	NSUN2	XP_002804365.1
Bos taurus (cattle)	NSUN2	XP_002696495.1
Rattus norvegicus (Norway rat)	Nsun2	NP_001101873.1
Danio rerio (zebrafish)	nsun2	NP_956005.1
Schizosaccharomyces pombe (fission yeast)	SPAC23C4.17	NP_593189.2
Magnaporthe oryzae (rice blast fungus)	MGG_13217	XP_003711942.1
Arabidopsis thaliana (thale cress)	AT2G22400	NP_850024.1
Xenopus tropicalis (tropical clawed frog)	nsun2	NP_001015962.1

Gene Ontology

The following NSUN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSUN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe30342	XM_535800.5 Latest version!	Canis lupus familiaris NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe30344	XM_005639683.2 Latest version!	Canis lupus familiaris NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OCe30344	XM_005639683.1	Canis lupus familiaris NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe30342	XM_535800.4	Canis lupus familiaris NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSUN2 ( XM_535800.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_535800.2 Canis lupus familiaris NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe30342
Clone ID Related Accession (Same CDS sequence)	XM_535800.5 , XM_535800.4
Accession Version	XM_535800.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535800.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC GGGCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCGCAGCGC 
GCGGAGGACG GCGCCGAGGG CGGCGGCAAG CGCCCGGAGG GGGCCTGGGA AGGCGGCTAT 
CCCGAGATCG TCAAGGAGAA CAAGCTCTTC GAGCACTACT ACCGGGAGCT CAAGATCGTG 
CCCGAGGGCG AGTGGGAGCA GTTCATGGGA GCGCTCAGGG AGCCCCTCCC GGCCACTTTG 
CGGATCACTG GCTACAAAAG CCACGCAAAG GAGATTCTTC ACTGCCTGAA GAACAAGTAT 
TTCAAGGAAT TGGAGGACCT GGAGGTGGAT GGTCAGAAAG TTGAAGTTCC ACAGCCCCTG 
AGTTGGTATC CTGAAGAACT TGCCTGGCAC ACAAATTTGA GTCGAAAAAT CTTGAGAAAG 
TCTCCACAAC TGGAGAAGTT TCACCAGTTT CTGGTTAGCG AAACCGAGTC TGGAAATATC 
AGTCGTCAAG AAGCTGTTAG CATGATTCCA CCACTGTTAC TCAATGCTCA CCCTCACCAT 
AAGATCTTAG ATATGTGTGC AGCACCTGGA TCAAAGACCA CACAGTTAAT TGAAATGTTA 
CATGCCGACA TGAATGTGCC TTTCCCAGAG GGGTTTGTCA TCGCAAATGA TGTGGACAAC 
AAGCGTTGCT ATCTGCTCGT TCATCAGGCC AAAAGGCTGA GCAGCCCCTG CATCATGGTG 
GTCAACCACG ATGCATCCAG CATACCTAGA CTCCAAATAG ATGTGAATGG GAGAAAAGAG 
ATTCTCTTCT ATGATCGCAT CTTATGTGAC GTCCCTTGCA GCGGAGATGG CACTATGAGA 
AAAAACATCG ATGTCTGGAA GAAGTGGACA ACCTTGAATA GCTTGCAGCT GCATGGCTTA 
CAGCTGCGGA TCGCAACGCG GGGTGCCGAG CAGCTGGTGG AGGGCGGCAG GATGGTGTAT 
TCTACTTGTT CACTAAACCC CATCGAAGAT GAAGCAGTTA TAGCATCTTT GCTGGAAAAG 
AGTGAAGGTG CTTTGGAACT TGCTGATGTG TCTTCTGAGT TGCCAGGGCT GAAGTGGATG 
CCCGGAATCA CCCATTGGAA GGTAATGACG AAAGATGGAC AGTGGTTTGC AGAGTGGGAA 
GATGTTCCTC ACAGCAGACA CACCCAGATC CGGCCCACCA TGTTCCCACC AAAGGACCCA 
GAAAAGTTAC AGGCGATGCA CCTTGAACGT TGTCTTAGGA TATTACCACA TCATCAGAAT 
ACTGGAGGGT TCTTTGTGGC AGTATTAGTG AAAAAGTCTT CAATGCCATG GAATAAACGT 
CCACCAAAGC TGCAGGTGGA GTCGGCGGAG CCCAGAGAGC CTGTGCGGGC CAGCCCTGCG 
CATCCCACAG GAGGAGCAGC TGCGGACCCC ACTGAGCCGG GAAGTAAAGC CGTCGGTGGG 
ATGGAGGACA CAGACACGAT GGAAAGGCCT GAGAATGTAG ACGACAGCGG AAGTAAGAGA 
GACGGAGTGT GTGGGCCGCC TCCATCTAAG AAAATGAAGT TGTTTGGATT TAAAGAAGAT 
CCGTTTGTGT TTATTCCTGA AGATGACCCG TTATTTCCAC CTATCCAGAA GTTTTATGCG 
TTGGATCCTT CATTCCCAAA GATGAATTTG TTAACTAGAA CTACAGAAGG GAAGAAGAGG 
CAGCTGTACA TGGTGTCTAA GGAGCTGAGG AACGTGCTGG TAAACAACAG CGAGCGGATG 
AAGGTTATAA ACACTGGGAT AAAAGTGTGG TGTCGGAATA ACAGCGGTGA AGAGTTCGAT 
TGTGCTTTTC GTCTGGCACA GGAGGGAATA TACACGTTGT ATCCATTTAT CAATTCAAGA 
ATTATCACTG TGTCGATGGA AGATGTTAAA ATTCTGTTAA CCCAGGAAAA TCCGTTTTTT 
AGAAAACTTA GCAGTGAGAC ATACAGCCAA GCCAAGGACT TGGCTAAGGG GAGCATCGTC 
CTGAAGTACG AGCCGGACCC TACGAAACCA GACACCCTCC AGTGCCCCAT TGTGCTATGT 
GGATGGCGGG GAAAGGCCTC CATTCGGACT TTTGTGCCCA AGAACGAACG GCTTCATTAT 
CTCAGGATGA TGGGGCTGGA GGTGTTGGCA GAAAAGAAGA AGGAGGGGAC TTCTCTGGCA 
CAGGAGAGTA CGGGCAGCTC TGGGCCGCCA GAGGACGAGG TGAGTGCTGA ACCCGGAGCA 
GACCCAGCAG CCAGTGTGGA CGCCAGCGCA GATGCCACCG TGGACACCCC CTCGGACGCC 
CCTTTGGACG CCAGCACGGG TTCCTCAGAC CCTGCCCCGA CGGAGACCTC CCTGCCCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535800.2
CDS	16..2358
Translation	MGRRARGRRLQQQQQQRPQRAEDGAEGGGKRPEGAWEGGYPEIVKENKLFEHYYRELKIVPEGEWEQFMGALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVNGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWMPGITHWKVMTKDGQWFAEWEDVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRPPKLQVESAEPREPVRASPAHPTGGAAADPTEPGSKAVGGMEDTDTMERPENVDDSGSKRDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIQKFYALDPSFPKMNLLTRTTEGKKRQLYMVSKELRNVLVNNSERMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDPTKPDTLQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLAEKKKEGTSLAQESTGSSGPPEDEVSAEPGADPAASVDASADATVDTPSDAPLDASTGSSDPAPTETSLPR

Target ORF information:

RefSeq Version	XM_535800.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535800.5

ORF Insert Sequence:

ATGGGGCGGC GGGCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCGCAGCGC 
GCGGAGGACG GCGCCGAGGG CGGCGGCAAG CGCCCGGAGG GGGCCTGGGA AGGCGGCTAT 
CCCGAGATCG TCAAGGAGAA CAAGCTCTTC GAGCACTACT ACCGGGAGCT CAAGATCGTG 
CCCGAGGGCG AGTGGGAGCA GTTCATGGGA GCGCTCAGGG AGCCCCTCCC GGCCACTTTG 
CGGATCACTG GCTACAAAAG CCACGCAAAG GAGATTCTTC ACTGCCTGAA GAACAAGTAT 
TTCAAGGAAT TGGAGGACCT GGAGGTGGAT GGTCAGAAAG TTGAAGTTCC ACAGCCCCTG 
AGTTGGTATC CTGAAGAACT TGCCTGGCAC ACAAATTTGA GTCGAAAAAT CTTGAGAAAG 
TCTCCACAAC TGGAGAAGTT TCACCAGTTT CTGGTTAGCG AAACCGAGTC TGGAAATATC 
AGTCGTCAAG AAGCTGTTAG CATGATTCCA CCACTGTTAC TCAATGCTCA CCCTCACCAT 
AAGATCTTAG ATATGTGTGC AGCACCTGGA TCAAAGACCA CACAGTTAAT TGAAATGTTA 
CATGCCGACA TGAATGTGCC TTTCCCAGAG GGGTTTGTCA TCGCAAATGA TGTGGACAAC 
AAGCGTTGCT ATCTGCTCGT TCATCAGGCC AAAAGGCTGA GCAGCCCCTG CATCATGGTG 
GTCAACCACG ATGCATCCAG CATACCTAGA CTCCAAATAG ATGTGAATGG GAGAAAAGAG 
ATTCTCTTCT ATGATCGCAT CTTATGTGAC GTCCCTTGCA GCGGAGATGG CACTATGAGA 
AAAAACATCG ATGTCTGGAA GAAGTGGACA ACCTTGAATA GCTTGCAGCT GCATGGCTTA 
CAGCTGCGGA TCGCAACGCG GGGTGCCGAG CAGCTGGTGG AGGGCGGCAG GATGGTGTAT 
TCTACTTGTT CACTAAACCC CATCGAAGAT GAAGCAGTTA TAGCATCTTT GCTGGAAAAG 
AGTGAAGGTG CTTTGGAACT TGCTGATGTG TCTTCTGAGT TGCCAGGGCT GAAGTGGATG 
CCCGGAATCA CCCATTGGAA GGTAATGACG AAAGATGGAC AGTGGTTTGC AGAGTGGGAA 
GATGTTCCTC ACAGCAGACA CACCCAGATC CGGCCCACCA TGTTCCCACC AAAGGACCCA 
GAAAAGTTAC AGGCGATGCA CCTTGAACGT TGTCTTAGGA TATTACCACA TCATCAGAAT 
ACTGGAGGGT TCTTTGTGGC AGTATTAGTG AAAAAGTCTT CAATGCCATG GAATAAACGT 
CCACCAAAGC TGCAGGTGGA GTCGGCGGAG CCCAGAGAGC CTGTGCGGGC CAGCCCTGCG 
CATCCCACAG GAGGAGCAGC TGCGGACCCC ACTGAGCCGG GAAGTAAAGC CGTCGGTGGG 
ATGGAGGACA CAGACACGAT GGAAAGGCCT GAGAATGTAG ACGACAGCGG AAGTAAGAGA 
GACGGAGTGT GTGGGCCGCC TCCATCTAAG AAAATGAAGT TGTTTGGATT TAAAGAAGAT 
CCGTTTGTGT TTATTCCTGA AGATGACCCG TTATTTCCAC CTATCCAGAA GTTTTATGCG 
TTGGATCCTT CATTCCCAAA GATGAATTTG TTAACTAGAA CTACAGAAGG GAAGAAGAGG 
CAGCTGTACA TGGTGTCTAA GGAGCTGAGG AACGTGCTGG TAAACAACAG CGAGCGGATG 
AAGGTTATAA ACACTGGGAT AAAAGTGTGG TGTCGGAATA ACAGCGGTGA AGAGTTCGAT 
TGTGCTTTTC GTCTGGCACA GGAGGGAATA TACACGTTGT ATCCATTTAT CAATTCAAGA 
ATTATCACTG TGTCGATGGA AGATGTTAAA ATTCTGTTAA CCCAGGAAAA TCCGTTTTTT 
AGAAAACTTA GCAGTGAGAC ATACAGCCAA GCCAAGGACT TGGCTAAGGG GAGCATCGTC 
CTGAAGTACG AGCCGGACCC TACGAAACCA GACACCCTCC AGTGCCCCAT TGTGCTATGT 
GGATGGCGGG GAAAGGCCTC CATTCGGACT TTTGTGCCCA AGAACGAACG GCTTCATTAT 
CTCAGGATGA TGGGGCTGGA GGTGTTGGCA GAAAAGAAGA AGGAGGGGAC TTCTCTGGCA 
CAGGAGAGTA CGGGCAGCTC TGGGCCGCCA GAGGACGAGG TGAGTGCTGA ACCCGGAGCA 
GACCCAGCAG CCAGTGTGGA CGCCAGCGCA GATGCCACCG TGGACACCCC CTCGGACGCC 
CCTTTGGACG CCAGCACGGG TTCCTCAGAC CCTGCCCCGA CGGAGACCTC CCTGCCCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( XM_535800.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_535800.2 Canis lupus familiaris NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe30342
Clone ID Related Accession (Same CDS sequence)	XM_535800.5 , XM_535800.4
Accession Version	XM_535800.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_535800.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC GGGCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCGCAGCGC 
GCGGAGGACG GCGCCGAGGG CGGCGGCAAG CGCCCGGAGG GGGCCTGGGA AGGCGGCTAT 
CCCGAGATCG TCAAGGAGAA CAAGCTCTTC GAGCACTACT ACCGGGAGCT CAAGATCGTG 
CCCGAGGGCG AGTGGGAGCA GTTCATGGGA GCGCTCAGGG AGCCCCTCCC GGCCACTTTG 
CGGATCACTG GCTACAAAAG CCACGCAAAG GAGATTCTTC ACTGCCTGAA GAACAAGTAT 
TTCAAGGAAT TGGAGGACCT GGAGGTGGAT GGTCAGAAAG TTGAAGTTCC ACAGCCCCTG 
AGTTGGTATC CTGAAGAACT TGCCTGGCAC ACAAATTTGA GTCGAAAAAT CTTGAGAAAG 
TCTCCACAAC TGGAGAAGTT TCACCAGTTT CTGGTTAGCG AAACCGAGTC TGGAAATATC 
AGTCGTCAAG AAGCTGTTAG CATGATTCCA CCACTGTTAC TCAATGCTCA CCCTCACCAT 
AAGATCTTAG ATATGTGTGC AGCACCTGGA TCAAAGACCA CACAGTTAAT TGAAATGTTA 
CATGCCGACA TGAATGTGCC TTTCCCAGAG GGGTTTGTCA TCGCAAATGA TGTGGACAAC 
AAGCGTTGCT ATCTGCTCGT TCATCAGGCC AAAAGGCTGA GCAGCCCCTG CATCATGGTG 
GTCAACCACG ATGCATCCAG CATACCTAGA CTCCAAATAG ATGTGAATGG GAGAAAAGAG 
ATTCTCTTCT ATGATCGCAT CTTATGTGAC GTCCCTTGCA GCGGAGATGG CACTATGAGA 
AAAAACATCG ATGTCTGGAA GAAGTGGACA ACCTTGAATA GCTTGCAGCT GCATGGCTTA 
CAGCTGCGGA TCGCAACGCG GGGTGCCGAG CAGCTGGTGG AGGGCGGCAG GATGGTGTAT 
TCTACTTGTT CACTAAACCC CATCGAAGAT GAAGCAGTTA TAGCATCTTT GCTGGAAAAG 
AGTGAAGGTG CTTTGGAACT TGCTGATGTG TCTTCTGAGT TGCCAGGGCT GAAGTGGATG 
CCCGGAATCA CCCATTGGAA GGTAATGACG AAAGATGGAC AGTGGTTTGC AGAGTGGGAA 
GATGTTCCTC ACAGCAGACA CACCCAGATC CGGCCCACCA TGTTCCCACC AAAGGACCCA 
GAAAAGTTAC AGGCGATGCA CCTTGAACGT TGTCTTAGGA TATTACCACA TCATCAGAAT 
ACTGGAGGGT TCTTTGTGGC AGTATTAGTG AAAAAGTCTT CAATGCCATG GAATAAACGT 
CCACCAAAGC TGCAGGTGGA GTCGGCGGAG CCCAGAGAGC CTGTGCGGGC CAGCCCTGCG 
CATCCCACAG GAGGAGCAGC TGCGGACCCC ACTGAGCCGG GAAGTAAAGC CGTCGGTGGG 
ATGGAGGACA CAGACACGAT GGAAAGGCCT GAGAATGTAG ACGACAGCGG AAGTAAGAGA 
GACGGAGTGT GTGGGCCGCC TCCATCTAAG AAAATGAAGT TGTTTGGATT TAAAGAAGAT 
CCGTTTGTGT TTATTCCTGA AGATGACCCG TTATTTCCAC CTATCCAGAA GTTTTATGCG 
TTGGATCCTT CATTCCCAAA GATGAATTTG TTAACTAGAA CTACAGAAGG GAAGAAGAGG 
CAGCTGTACA TGGTGTCTAA GGAGCTGAGG AACGTGCTGG TAAACAACAG CGAGCGGATG 
AAGGTTATAA ACACTGGGAT AAAAGTGTGG TGTCGGAATA ACAGCGGTGA AGAGTTCGAT 
TGTGCTTTTC GTCTGGCACA GGAGGGAATA TACACGTTGT ATCCATTTAT CAATTCAAGA 
ATTATCACTG TGTCGATGGA AGATGTTAAA ATTCTGTTAA CCCAGGAAAA TCCGTTTTTT 
AGAAAACTTA GCAGTGAGAC ATACAGCCAA GCCAAGGACT TGGCTAAGGG GAGCATCGTC 
CTGAAGTACG AGCCGGACCC TACGAAACCA GACACCCTCC AGTGCCCCAT TGTGCTATGT 
GGATGGCGGG GAAAGGCCTC CATTCGGACT TTTGTGCCCA AGAACGAACG GCTTCATTAT 
CTCAGGATGA TGGGGCTGGA GGTGTTGGCA GAAAAGAAGA AGGAGGGGAC TTCTCTGGCA 
CAGGAGAGTA CGGGCAGCTC TGGGCCGCCA GAGGACGAGG TGAGTGCTGA ACCCGGAGCA 
GACCCAGCAG CCAGTGTGGA CGCCAGCGCA GATGCCACCG TGGACACCCC CTCGGACGCC 
CCTTTGGACG CCAGCACGGG TTCCTCAGAC CCTGCCCCGA CGGAGACCTC CCTGCCCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535800.2
CDS	250..2592
Translation	MGRRARGRRLQQQQQQRPQRAEDGAEGGGKRPEGAWEGGYPEIVKENKLFEHYYRELKIVPEGEWEQFMGALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVNGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWMPGITHWKVMTKDGQWFAEWEDVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRPPKLQVESAEPREPVRASPAHPTGGAAADPTEPGSKAVGGMEDTDTMERPENVDDSGSKRDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIQKFYALDPSFPKMNLLTRTTEGKKRQLYMVSKELRNVLVNNSERMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDPTKPDTLQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLAEKKKEGTSLAQESTGSSGPPEDEVSAEPGADPAASVDASADATVDTPSDAPLDASTGSSDPAPTETSLPR

Target ORF information:

RefSeq Version	XM_535800.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535800.4

ORF Insert Sequence:

ATGGGGCGGC GGGCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCGCAGCGC 
GCGGAGGACG GCGCCGAGGG CGGCGGCAAG CGCCCGGAGG GGGCCTGGGA AGGCGGCTAT 
CCCGAGATCG TCAAGGAGAA CAAGCTCTTC GAGCACTACT ACCGGGAGCT CAAGATCGTG 
CCCGAGGGCG AGTGGGAGCA GTTCATGGGA GCGCTCAGGG AGCCCCTCCC GGCCACTTTG 
CGGATCACTG GCTACAAAAG CCACGCAAAG GAGATTCTTC ACTGCCTGAA GAACAAGTAT 
TTCAAGGAAT TGGAGGACCT GGAGGTGGAT GGTCAGAAAG TTGAAGTTCC ACAGCCCCTG 
AGTTGGTATC CTGAAGAACT TGCCTGGCAC ACAAATTTGA GTCGAAAAAT CTTGAGAAAG 
TCTCCACAAC TGGAGAAGTT TCACCAGTTT CTGGTTAGCG AAACCGAGTC TGGAAATATC 
AGTCGTCAAG AAGCTGTTAG CATGATTCCA CCACTGTTAC TCAATGCTCA CCCTCACCAT 
AAGATCTTAG ATATGTGTGC AGCACCTGGA TCAAAGACCA CACAGTTAAT TGAAATGTTA 
CATGCCGACA TGAATGTGCC TTTCCCAGAG GGGTTTGTCA TCGCAAATGA TGTGGACAAC 
AAGCGTTGCT ATCTGCTCGT TCATCAGGCC AAAAGGCTGA GCAGCCCCTG CATCATGGTG 
GTCAACCACG ATGCATCCAG CATACCTAGA CTCCAAATAG ATGTGAATGG GAGAAAAGAG 
ATTCTCTTCT ATGATCGCAT CTTATGTGAC GTCCCTTGCA GCGGAGATGG CACTATGAGA 
AAAAACATCG ATGTCTGGAA GAAGTGGACA ACCTTGAATA GCTTGCAGCT GCATGGCTTA 
CAGCTGCGGA TCGCAACGCG GGGTGCCGAG CAGCTGGTGG AGGGCGGCAG GATGGTGTAT 
TCTACTTGTT CACTAAACCC CATCGAAGAT GAAGCAGTTA TAGCATCTTT GCTGGAAAAG 
AGTGAAGGTG CTTTGGAACT TGCTGATGTG TCTTCTGAGT TGCCAGGGCT GAAGTGGATG 
CCCGGAATCA CCCATTGGAA GGTAATGACG AAAGATGGAC AGTGGTTTGC AGAGTGGGAA 
GATGTTCCTC ACAGCAGACA CACCCAGATC CGGCCCACCA TGTTCCCACC AAAGGACCCA 
GAAAAGTTAC AGGCGATGCA CCTTGAACGT TGTCTTAGGA TATTACCACA TCATCAGAAT 
ACTGGAGGGT TCTTTGTGGC AGTATTAGTG AAAAAGTCTT CAATGCCATG GAATAAACGT 
CCACCAAAGC TGCAGGTGGA GTCGGCGGAG CCCAGAGAGC CTGTGCGGGC CAGCCCTGCG 
CATCCCACAG GAGGAGCAGC TGCGGACCCC ACTGAGCCGG GAAGTAAAGC CGTCGGTGGG 
ATGGAGGACA CAGACACGAT GGAAAGGCCT GAGAATGTAG ACGACAGCGG AAGTAAGAGA 
GACGGAGTGT GTGGGCCGCC TCCATCTAAG AAAATGAAGT TGTTTGGATT TAAAGAAGAT 
CCGTTTGTGT TTATTCCTGA AGATGACCCG TTATTTCCAC CTATCCAGAA GTTTTATGCG 
TTGGATCCTT CATTCCCAAA GATGAATTTG TTAACTAGAA CTACAGAAGG GAAGAAGAGG 
CAGCTGTACA TGGTGTCTAA GGAGCTGAGG AACGTGCTGG TAAACAACAG CGAGCGGATG 
AAGGTTATAA ACACTGGGAT AAAAGTGTGG TGTCGGAATA ACAGCGGTGA AGAGTTCGAT 
TGTGCTTTTC GTCTGGCACA GGAGGGAATA TACACGTTGT ATCCATTTAT CAATTCAAGA 
ATTATCACTG TGTCGATGGA AGATGTTAAA ATTCTGTTAA CCCAGGAAAA TCCGTTTTTT 
AGAAAACTTA GCAGTGAGAC ATACAGCCAA GCCAAGGACT TGGCTAAGGG GAGCATCGTC 
CTGAAGTACG AGCCGGACCC TACGAAACCA GACACCCTCC AGTGCCCCAT TGTGCTATGT 
GGATGGCGGG GAAAGGCCTC CATTCGGACT TTTGTGCCCA AGAACGAACG GCTTCATTAT 
CTCAGGATGA TGGGGCTGGA GGTGTTGGCA GAAAAGAAGA AGGAGGGGAC TTCTCTGGCA 
CAGGAGAGTA CGGGCAGCTC TGGGCCGCCA GAGGACGAGG TGAGTGCTGA ACCCGGAGCA 
GACCCAGCAG CCAGTGTGGA CGCCAGCGCA GATGCCACCG TGGACACCCC CTCGGACGCC 
CCTTTGGACG CCAGCACGGG TTCCTCAGAC CCTGCCCCGA CGGAGACCTC CCTGCCCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( XM_005639683.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005639740.1 Canis lupus familiaris NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X2, mRNA.

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CloneID	OCe30344
Clone ID Related Accession (Same CDS sequence)	XM_005639683.1 , XM_005639683.2
Accession Version	XM_005639683.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC GGGCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCGCAGCGC 
GCGGAGGACG GCGCCGAGGG CGGCGGCAAG CGCCCGGAGG GGGCCTGGGA AGGCGGCTAT 
CCCGAGATCG TCAAGGAGAA CAAGCTCTTC GAGCACTACT ACCGGGAGCT CAAGATCGTG 
CCCGAGGGCG AGTGGGAGCA GTTCATGGGA GCGCTCAGGG AGCCCCTCCC GGCCACTTTG 
CGGATCACTG GCTACAAAAG CCACGCAAAG GAGATTCTTC ACTGCCTGAA GAACAAGTAT 
TTCAAGGAAT TGGAGGACCT GGAGGTGGAT GGTCAGAAAG TTGAAGTTCC ACAGCCCCTG 
AGTTGCGGAG ATGGCACTAT GAGAAAAAAC ATCGATGTCT GGAAGAAGTG GACAACCTTG 
AATAGCTTGC AGCTGCATGG CTTACAGCTG CGGATCGCAA CGCGGGGTGC CGAGCAGCTG 
GTGGAGGGCG GCAGGATGGT GTATTCTACT TGTTCACTAA ACCCCATCGA AGATGAAGCA 
GTTATAGCAT CTTTGCTGGA AAAGAGTGAA GGTGCTTTGG AACTTGCTGA TGTGTCTTCT 
GAGTTGCCAG GGCTGAAGTG GATGCCCGGA ATCACCCATT GGAAGGTAAT GACGAAAGAT 
GGACAGTGGT TTGCAGAGTG GGAAGATGTT CCTCACAGCA GACACACCCA GATCCGGCCC 
ACCATGTTCC CACCAAAGGA CCCAGAAAAG TTACAGGCGA TGCACCTTGA ACGTTGTCTT 
AGGATATTAC CACATCATCA GAATACTGGA GGGTTCTTTG TGGCAGTATT AGTGAAAAAG 
TCTTCAATGC CATGGAATAA ACGTCCACCA AAGCTGCAGG TGGAGTCGGC GGAGCCCAGA 
GAGCCTGTGC GGGCCAGCCC TGCGCATCCC ACAGGAGGAG CAGCTGCGGA CCCCACTGAG 
CCGGGAAGTA AAGCCGTCGG TGGGATGGAG GACACAGACA CGATGGAAAG GCCTGAGAAT 
GTAGACGACA GCGGAAGTAA GAGAGACGGA GTGTGTGGGC CGCCTCCATC TAAGAAAATG 
AAGTTGTTTG GATTTAAAGA AGATCCGTTT GTGTTTATTC CTGAAGATGA CCCGTTATTT 
CCACCTATCC AGAAGTTTTA TGCGTTGGAT CCTTCATTCC CAAAGATGAA TTTGTTAACT 
AGAACTACAG AAGGGAAGAA GAGGCAGCTG TACATGGTGT CTAAGGAGCT GAGGAACGTG 
CTGGTAAACA ACAGCGAGCG GATGAAGGTT ATAAACACTG GGATAAAAGT GTGGTGTCGG 
AATAACAGCG GTGAAGAGTT CGATTGTGCT TTTCGTCTGG CACAGGAGGG AATATACACG 
TTGTATCCAT TTATCAATTC AAGAATTATC ACTGTGTCGA TGGAAGATGT TAAAATTCTG 
TTAACCCAGG AAAATCCGTT TTTTAGAAAA CTTAGCAGTG AGACATACAG CCAAGCCAAG 
GACTTGGCTA AGGGGAGCAT CGTCCTGAAG TACGAGCCGG ACCCTACGAA ACCAGACACC 
CTCCAGTGCC CCATTGTGCT ATGTGGATGG CGGGGAAAGG CCTCCATTCG GACTTTTGTG 
CCCAAGAACG AACGGCTTCA TTATCTCAGG ATGATGGGGC TGGAGGTGTT GGCAGAAAAG 
AAGAAGGAGG GGACTTCTCT GGCACAGGAG AGTACGGGCA GCTCTGGGCC GCCAGAGGAC 
GAGGTGAGTG CTGAACCCGG AGCAGACCCA GCAGCCAGTG TGGACGCCAG CGCAGATGCC 
ACCGTGGACA CCCCCTCGGA CGCCCCTTTG GACGCCAGCA CGGGTTCCTC AGACCCTGCC 
CCGACGGAGA CCTCCCTGCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639740.1
CDS	250..2136
Translation	MGRRARGRRLQQQQQQRPQRAEDGAEGGGKRPEGAWEGGYPEIVKENKLFEHYYRELKIVPEGEWEQFMGALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSCGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWMPGITHWKVMTKDGQWFAEWEDVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRPPKLQVESAEPREPVRASPAHPTGGAAADPTEPGSKAVGGMEDTDTMERPENVDDSGSKRDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIQKFYALDPSFPKMNLLTRTTEGKKRQLYMVSKELRNVLVNNSERMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDPTKPDTLQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLAEKKKEGTSLAQESTGSSGPPEDEVSAEPGADPAASVDASADATVDTPSDAPLDASTGSSDPAPTETSLPR

Target ORF information:

RefSeq Version	XM_005639683.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639683.1

ORF Insert Sequence:

ATGGGGCGGC GGGCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCGCAGCGC 
GCGGAGGACG GCGCCGAGGG CGGCGGCAAG CGCCCGGAGG GGGCCTGGGA AGGCGGCTAT 
CCCGAGATCG TCAAGGAGAA CAAGCTCTTC GAGCACTACT ACCGGGAGCT CAAGATCGTG 
CCCGAGGGCG AGTGGGAGCA GTTCATGGGA GCGCTCAGGG AGCCCCTCCC GGCCACTTTG 
CGGATCACTG GCTACAAAAG CCACGCAAAG GAGATTCTTC ACTGCCTGAA GAACAAGTAT 
TTCAAGGAAT TGGAGGACCT GGAGGTGGAT GGTCAGAAAG TTGAAGTTCC ACAGCCCCTG 
AGTTGCGGAG ATGGCACTAT GAGAAAAAAC ATCGATGTCT GGAAGAAGTG GACAACCTTG 
AATAGCTTGC AGCTGCATGG CTTACAGCTG CGGATCGCAA CGCGGGGTGC CGAGCAGCTG 
GTGGAGGGCG GCAGGATGGT GTATTCTACT TGTTCACTAA ACCCCATCGA AGATGAAGCA 
GTTATAGCAT CTTTGCTGGA AAAGAGTGAA GGTGCTTTGG AACTTGCTGA TGTGTCTTCT 
GAGTTGCCAG GGCTGAAGTG GATGCCCGGA ATCACCCATT GGAAGGTAAT GACGAAAGAT 
GGACAGTGGT TTGCAGAGTG GGAAGATGTT CCTCACAGCA GACACACCCA GATCCGGCCC 
ACCATGTTCC CACCAAAGGA CCCAGAAAAG TTACAGGCGA TGCACCTTGA ACGTTGTCTT 
AGGATATTAC CACATCATCA GAATACTGGA GGGTTCTTTG TGGCAGTATT AGTGAAAAAG 
TCTTCAATGC CATGGAATAA ACGTCCACCA AAGCTGCAGG TGGAGTCGGC GGAGCCCAGA 
GAGCCTGTGC GGGCCAGCCC TGCGCATCCC ACAGGAGGAG CAGCTGCGGA CCCCACTGAG 
CCGGGAAGTA AAGCCGTCGG TGGGATGGAG GACACAGACA CGATGGAAAG GCCTGAGAAT 
GTAGACGACA GCGGAAGTAA GAGAGACGGA GTGTGTGGGC CGCCTCCATC TAAGAAAATG 
AAGTTGTTTG GATTTAAAGA AGATCCGTTT GTGTTTATTC CTGAAGATGA CCCGTTATTT 
CCACCTATCC AGAAGTTTTA TGCGTTGGAT CCTTCATTCC CAAAGATGAA TTTGTTAACT 
AGAACTACAG AAGGGAAGAA GAGGCAGCTG TACATGGTGT CTAAGGAGCT GAGGAACGTG 
CTGGTAAACA ACAGCGAGCG GATGAAGGTT ATAAACACTG GGATAAAAGT GTGGTGTCGG 
AATAACAGCG GTGAAGAGTT CGATTGTGCT TTTCGTCTGG CACAGGAGGG AATATACACG 
TTGTATCCAT TTATCAATTC AAGAATTATC ACTGTGTCGA TGGAAGATGT TAAAATTCTG 
TTAACCCAGG AAAATCCGTT TTTTAGAAAA CTTAGCAGTG AGACATACAG CCAAGCCAAG 
GACTTGGCTA AGGGGAGCAT CGTCCTGAAG TACGAGCCGG ACCCTACGAA ACCAGACACC 
CTCCAGTGCC CCATTGTGCT ATGTGGATGG CGGGGAAAGG CCTCCATTCG GACTTTTGTG 
CCCAAGAACG AACGGCTTCA TTATCTCAGG ATGATGGGGC TGGAGGTGTT GGCAGAAAAG 
AAGAAGGAGG GGACTTCTCT GGCACAGGAG AGTACGGGCA GCTCTGGGCC GCCAGAGGAC 
GAGGTGAGTG CTGAACCCGG AGCAGACCCA GCAGCCAGTG TGGACGCCAG CGCAGATGCC 
ACCGTGGACA CCCCCTCGGA CGCCCCTTTG GACGCCAGCA CGGGTTCCTC AGACCCTGCC 
CCGACGGAGA CCTCCCTGCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( XM_005639683.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005639740.1 Canis lupus familiaris NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant X2, mRNA.

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CloneID	OCe30344
Clone ID Related Accession (Same CDS sequence)	XM_005639683.1 , XM_005639683.2
Accession Version	XM_005639683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC GGGCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCGCAGCGC 
GCGGAGGACG GCGCCGAGGG CGGCGGCAAG CGCCCGGAGG GGGCCTGGGA AGGCGGCTAT 
CCCGAGATCG TCAAGGAGAA CAAGCTCTTC GAGCACTACT ACCGGGAGCT CAAGATCGTG 
CCCGAGGGCG AGTGGGAGCA GTTCATGGGA GCGCTCAGGG AGCCCCTCCC GGCCACTTTG 
CGGATCACTG GCTACAAAAG CCACGCAAAG GAGATTCTTC ACTGCCTGAA GAACAAGTAT 
TTCAAGGAAT TGGAGGACCT GGAGGTGGAT GGTCAGAAAG TTGAAGTTCC ACAGCCCCTG 
AGTTGCGGAG ATGGCACTAT GAGAAAAAAC ATCGATGTCT GGAAGAAGTG GACAACCTTG 
AATAGCTTGC AGCTGCATGG CTTACAGCTG CGGATCGCAA CGCGGGGTGC CGAGCAGCTG 
GTGGAGGGCG GCAGGATGGT GTATTCTACT TGTTCACTAA ACCCCATCGA AGATGAAGCA 
GTTATAGCAT CTTTGCTGGA AAAGAGTGAA GGTGCTTTGG AACTTGCTGA TGTGTCTTCT 
GAGTTGCCAG GGCTGAAGTG GATGCCCGGA ATCACCCATT GGAAGGTAAT GACGAAAGAT 
GGACAGTGGT TTGCAGAGTG GGAAGATGTT CCTCACAGCA GACACACCCA GATCCGGCCC 
ACCATGTTCC CACCAAAGGA CCCAGAAAAG TTACAGGCGA TGCACCTTGA ACGTTGTCTT 
AGGATATTAC CACATCATCA GAATACTGGA GGGTTCTTTG TGGCAGTATT AGTGAAAAAG 
TCTTCAATGC CATGGAATAA ACGTCCACCA AAGCTGCAGG TGGAGTCGGC GGAGCCCAGA 
GAGCCTGTGC GGGCCAGCCC TGCGCATCCC ACAGGAGGAG CAGCTGCGGA CCCCACTGAG 
CCGGGAAGTA AAGCCGTCGG TGGGATGGAG GACACAGACA CGATGGAAAG GCCTGAGAAT 
GTAGACGACA GCGGAAGTAA GAGAGACGGA GTGTGTGGGC CGCCTCCATC TAAGAAAATG 
AAGTTGTTTG GATTTAAAGA AGATCCGTTT GTGTTTATTC CTGAAGATGA CCCGTTATTT 
CCACCTATCC AGAAGTTTTA TGCGTTGGAT CCTTCATTCC CAAAGATGAA TTTGTTAACT 
AGAACTACAG AAGGGAAGAA GAGGCAGCTG TACATGGTGT CTAAGGAGCT GAGGAACGTG 
CTGGTAAACA ACAGCGAGCG GATGAAGGTT ATAAACACTG GGATAAAAGT GTGGTGTCGG 
AATAACAGCG GTGAAGAGTT CGATTGTGCT TTTCGTCTGG CACAGGAGGG AATATACACG 
TTGTATCCAT TTATCAATTC AAGAATTATC ACTGTGTCGA TGGAAGATGT TAAAATTCTG 
TTAACCCAGG AAAATCCGTT TTTTAGAAAA CTTAGCAGTG AGACATACAG CCAAGCCAAG 
GACTTGGCTA AGGGGAGCAT CGTCCTGAAG TACGAGCCGG ACCCTACGAA ACCAGACACC 
CTCCAGTGCC CCATTGTGCT ATGTGGATGG CGGGGAAAGG CCTCCATTCG GACTTTTGTG 
CCCAAGAACG AACGGCTTCA TTATCTCAGG ATGATGGGGC TGGAGGTGTT GGCAGAAAAG 
AAGAAGGAGG GGACTTCTCT GGCACAGGAG AGTACGGGCA GCTCTGGGCC GCCAGAGGAC 
GAGGTGAGTG CTGAACCCGG AGCAGACCCA GCAGCCAGTG TGGACGCCAG CGCAGATGCC 
ACCGTGGACA CCCCCTCGGA CGCCCCTTTG GACGCCAGCA CGGGTTCCTC AGACCCTGCC 
CCGACGGAGA CCTCCCTGCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639740.1
CDS	16..1902
Translation	MGRRARGRRLQQQQQQRPQRAEDGAEGGGKRPEGAWEGGYPEIVKENKLFEHYYRELKIVPEGEWEQFMGALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSCGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWMPGITHWKVMTKDGQWFAEWEDVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRPPKLQVESAEPREPVRASPAHPTGGAAADPTEPGSKAVGGMEDTDTMERPENVDDSGSKRDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIQKFYALDPSFPKMNLLTRTTEGKKRQLYMVSKELRNVLVNNSERMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDPTKPDTLQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLAEKKKEGTSLAQESTGSSGPPEDEVSAEPGADPAASVDASADATVDTPSDAPLDASTGSSDPAPTETSLPR

Target ORF information:

RefSeq Version	XM_005639683.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639683.2

ORF Insert Sequence:

ATGGGGCGGC GGGCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCGCAGCGC 
GCGGAGGACG GCGCCGAGGG CGGCGGCAAG CGCCCGGAGG GGGCCTGGGA AGGCGGCTAT 
CCCGAGATCG TCAAGGAGAA CAAGCTCTTC GAGCACTACT ACCGGGAGCT CAAGATCGTG 
CCCGAGGGCG AGTGGGAGCA GTTCATGGGA GCGCTCAGGG AGCCCCTCCC GGCCACTTTG 
CGGATCACTG GCTACAAAAG CCACGCAAAG GAGATTCTTC ACTGCCTGAA GAACAAGTAT 
TTCAAGGAAT TGGAGGACCT GGAGGTGGAT GGTCAGAAAG TTGAAGTTCC ACAGCCCCTG 
AGTTGCGGAG ATGGCACTAT GAGAAAAAAC ATCGATGTCT GGAAGAAGTG GACAACCTTG 
AATAGCTTGC AGCTGCATGG CTTACAGCTG CGGATCGCAA CGCGGGGTGC CGAGCAGCTG 
GTGGAGGGCG GCAGGATGGT GTATTCTACT TGTTCACTAA ACCCCATCGA AGATGAAGCA 
GTTATAGCAT CTTTGCTGGA AAAGAGTGAA GGTGCTTTGG AACTTGCTGA TGTGTCTTCT 
GAGTTGCCAG GGCTGAAGTG GATGCCCGGA ATCACCCATT GGAAGGTAAT GACGAAAGAT 
GGACAGTGGT TTGCAGAGTG GGAAGATGTT CCTCACAGCA GACACACCCA GATCCGGCCC 
ACCATGTTCC CACCAAAGGA CCCAGAAAAG TTACAGGCGA TGCACCTTGA ACGTTGTCTT 
AGGATATTAC CACATCATCA GAATACTGGA GGGTTCTTTG TGGCAGTATT AGTGAAAAAG 
TCTTCAATGC CATGGAATAA ACGTCCACCA AAGCTGCAGG TGGAGTCGGC GGAGCCCAGA 
GAGCCTGTGC GGGCCAGCCC TGCGCATCCC ACAGGAGGAG CAGCTGCGGA CCCCACTGAG 
CCGGGAAGTA AAGCCGTCGG TGGGATGGAG GACACAGACA CGATGGAAAG GCCTGAGAAT 
GTAGACGACA GCGGAAGTAA GAGAGACGGA GTGTGTGGGC CGCCTCCATC TAAGAAAATG 
AAGTTGTTTG GATTTAAAGA AGATCCGTTT GTGTTTATTC CTGAAGATGA CCCGTTATTT 
CCACCTATCC AGAAGTTTTA TGCGTTGGAT CCTTCATTCC CAAAGATGAA TTTGTTAACT 
AGAACTACAG AAGGGAAGAA GAGGCAGCTG TACATGGTGT CTAAGGAGCT GAGGAACGTG 
CTGGTAAACA ACAGCGAGCG GATGAAGGTT ATAAACACTG GGATAAAAGT GTGGTGTCGG 
AATAACAGCG GTGAAGAGTT CGATTGTGCT TTTCGTCTGG CACAGGAGGG AATATACACG 
TTGTATCCAT TTATCAATTC AAGAATTATC ACTGTGTCGA TGGAAGATGT TAAAATTCTG 
TTAACCCAGG AAAATCCGTT TTTTAGAAAA CTTAGCAGTG AGACATACAG CCAAGCCAAG 
GACTTGGCTA AGGGGAGCAT CGTCCTGAAG TACGAGCCGG ACCCTACGAA ACCAGACACC 
CTCCAGTGCC CCATTGTGCT ATGTGGATGG CGGGGAAAGG CCTCCATTCG GACTTTTGTG 
CCCAAGAACG AACGGCTTCA TTATCTCAGG ATGATGGGGC TGGAGGTGTT GGCAGAAAAG 
AAGAAGGAGG GGACTTCTCT GGCACAGGAG AGTACGGGCA GCTCTGGGCC GCCAGAGGAC 
GAGGTGAGTG CTGAACCCGG AGCAGACCCA GCAGCCAGTG TGGACGCCAG CGCAGATGCC 
ACCGTGGACA CCCCCTCGGA CGCCCCTTTG GACGCCAGCA CGGGTTCCTC AGACCCTGCC 
CCGACGGAGA CCTCCCTGCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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NSUN2 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム